Affinage

SLAMF1

Signaling lymphocytic activation molecule · UniProt Q13291

Length
335 aa
Mass
37.2 kDa
Annotated
2026-06-10
100 papers in source corpus 32 papers cited in narrative 32 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SLAMF1 (CD150/SLAM) is a hematopoietic Ig-superfamily receptor that operates both as a costimulatory/innate immune signaling receptor and as the entry receptor for morbilliviruses (PMID:10972291, PMID:15123745). Its membrane-distal V domain is necessary and sufficient for measles virus receptor function, with residues around positions 58–67 (especially His61) determining the human-versus-mouse species barrier (PMID:12917459); SLAM serves as a common receptor for all measles strains tested and binds hemagglutinin at a site distinct from CD46 (PMID:12029158). SLAM recognition drives macropinocytosis-like viral uptake and triggers receptor-specific conformational changes in hemagglutinin that license membrane fusion (PMID:18292085, PMID:28100610), and SLAM-blind morbiliviruses are avirulent in ferret and primate models, establishing receptor engagement as essential for pathogenesis (PMID:16731947, PMID:20071568). In signaling, SLAMF1 is tyrosine-phosphorylated by Src-family kinases Lck/Fyn on cytoplasmic ITSM tyrosines Y281 and Y327, which recruit the adaptor SAP/SH2D1A; SAP binds these motifs and governs the balance of SHIP versus SHP-2/SHP-1 phosphatase access, while SAP also binds and activates FynT to propagate signaling (PMID:11313386, PMID:11806999, PMID:15096483). This SLAM–SAP–Fyn axis mediates homotypic costimulation during NKT/iNKT cell selection and is required for IL-4 production by germinal center T follicular helper cells and for cytotoxic synapse organization (PMID:18031695, PMID:20525889, PMID:22683123, PMID:30833791). Downstream, SLAMF1 ligation activates ERK (via SHIP) and Akt (via Syk/SAP) pathways and controls cytokine output (PMID:15315965). As a TLR4 co-receptor, SLAMF1 regulates LPS-induced IL-12/TNF/NO production and, in human macrophages, traffics from the endocytic recycling compartment with TRAM to bacterial phagosomes in a Rab11-dependent manner to enable TRIF-dependent IFNβ induction and Gram-negative bacterial killing (PMID:15123745, PMID:29440514). In phagosomes SLAMF1 recruits a Beclin-1/Vps34/UVRAG complex through its cytoplasmic tail to regulate NOX2 activity, phagolysosomal maturation, and autophagy (PMID:22493499, PMID:26619119). These functions place SLAMF1 at the center of host defense against intracellular pathogens including Leishmania and Trypanosoma cruzi (PMID:15123745, PMID:22807679).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1993 Medium

    Before its identity was known, the antigen IPO-3 was characterized as an activation-induced glycoprotein with signaling capacity, establishing that SLAMF1 transduces lymphocyte activation signals.

    Evidence Monoclonal antibody characterization with in vitro kinase, calcium flux, and proliferation assays on activated B cells

    PMID:8409422

    Open questions at the time
    • Molecular identity and cytoplasmic signaling motifs not yet defined
    • Associated kinase not identified
  2. 2000 High

    The question of which receptor measles virus uses was answered by showing SLAM confers susceptibility, defining SLAMF1 as a virus entry receptor.

    Evidence Transfection of human SLAM cDNA into non-susceptible cells with virus binding and replication assays

    PMID:10972291

    Open questions at the time
    • Binding domain on SLAM not mapped
    • Post-binding entry mechanism unknown
  3. 2001 High

    How SLAMF1 couples to intracellular signaling was established by identifying ITSM tyrosines that recruit SAP, SHIP, and SHP-2, defining the adaptor switch logic.

    Evidence Co-IP and GST pulldown with Y-to-F mutants of the cytoplasmic tail

    PMID:11313386

    Open questions at the time
    • Kinases responsible for tyrosine phosphorylation not identified here
    • Functional consequences of phosphatase switching untested
  4. 2002 High

    The kinases and dual binding modes governing SLAM signaling were resolved, showing Lck/Fyn phosphorylate the tail and SAP binds Y281/Y327 by phospho-dependent and phospho-independent modes.

    Evidence In vitro kinase assay, co-localization, co-IP, and site-directed mutagenesis

    PMID:11806999

    Open questions at the time
    • Downstream effectors of SAP not yet defined
    • Quantitative contribution of each binding mode in vivo unknown
  5. 2002 High

    SLAM was shown to be the universal measles receptor across strains while CD46 usage maps to a separable hemagglutinin determinant, distinguishing the two receptor binding sites.

    Evidence Infection of CHO cells expressing CD46 or SLAM with a strain panel plus recombinant mutant virus

    PMID:12029158

    Open questions at the time
    • Atomic-level SLAM-binding interface not resolved here
  6. 2003 High

    The receptor-determining region was mapped to the SLAM V domain, with residues 58–67 and His61 explaining the human/mouse species barrier.

    Evidence Human/mouse chimeric SLAM constructs in VSV pseudotype assays with mutagenesis

    PMID:12917459

    Open questions at the time
    • Structural basis of His61 contribution not solved
    • Other ectodomain contacts not excluded
  7. 2004 High

    The downstream output of SAP was defined as direct FynT activation, linking SLAM engagement to a catalytic signaling event.

    Evidence Yeast two-hybrid, GST pulldown, and in vitro kinase assay with SAP R78E mutant

    PMID:15096483

    Open questions at the time
    • Substrates of activated FynT downstream of SLAM not enumerated
  8. 2004 High

    Genetic deletion established SLAMF1 as a TLR4 co-receptor and an in vivo determinant of macrophage antimicrobial function and T cell cytokine polarization.

    Evidence Targeted knockout with cytokine assays and Leishmania major infection model

    PMID:15123745

    Open questions at the time
    • Molecular mechanism linking SLAM to TLR4 signaling not defined here
    • TLR-specificity rationale unexplained
  9. 2004 High

    Branch-specific signaling logic was dissected, showing ERK activation depends on SHIP while Akt depends on Syk/SAP, placing SLAM signaling components in pathway order.

    Evidence DT40 B-cell sublines deficient in SHIP, SAP, Syk, Lyn, or Btk with phospho-Western analysis

    PMID:15315965

    Open questions at the time
    • Cross-talk between ERK and Akt branches not resolved
    • In vivo relevance of each branch untested
  10. 2007 High

    Homotypic SLAMF1/SLAMF6 engagement was shown to provide costimulation during NKT selection, defining a physiological ligand context for SLAM signaling.

    Evidence Genetic knockouts and epistasis in in vivo NKT development assays

    PMID:18031695

    Open questions at the time
    • Relative contribution of SLAMF1 versus SLAMF6 not separated
  11. 2008 High

    The post-binding step was clarified by showing distinct hemagglutinin residues for SLAM binding versus SLAM-triggered fusion, revealing receptor-induced conformational change.

    Evidence Surface plasmon resonance, fusion assays, and crystal-structure-guided mutagenesis

    PMID:18292085

    Open questions at the time
    • Conformational intermediate of the fusion machinery not structurally captured
  12. 2010 High

    SLAM-blind recombinant viruses formally proved SLAM recognition is required for measles virulence in primates, and SLAM signaling was shown to be selectively required for GC TFH IL-4 production.

    Evidence R533A SLAM-blind MV in rhesus monkeys; SAP/SLAM-deficient mice with GC TFH cytokine staining

    PMID:20071568 PMID:20525889

    Open questions at the time
    • Identity of any low-level SLAM-independent entry route in vivo unclear
    • Mechanism restricting SLAM signaling to IL-4 versus differentiation not defined
  13. 2012 High

    A non-canonical, ITAM-independent SLAM function was uncovered: recruitment of a Beclin-1/Vps34/UVRAG complex to phagosomes to control NOX2 and phagolysosomal maturation.

    Evidence Co-IP, domain-deletion pulldowns, and NOX2 activity assay in Beclin-1+/- macrophages

    PMID:22493499

    Open questions at the time
    • How the cytoplasmic tail engages Beclin-1 structurally unknown
    • Relationship to the SAP signaling branch unclear
  14. 2015 Medium

    SLAMF1 was linked to autophagy regulation and migratory control in CLL and myeloid cells, extending its role to tumor-cell autophagic flux and inflammation-associated cell movement.

    Evidence siRNA knockdown and agonistic ligation with co-IP and chemotaxis/autophagy assays; Slamf1-/- and Slamf8-/- migration models

    PMID:25799045 PMID:26619119

    Open questions at the time
    • Mechanistic link between SLAM-driven ROS and migration not fully resolved
    • Single-lab migration findings
  15. 2018 High

    The mechanism by which SLAMF1 enables TLR4 IFNβ signaling was defined as Rab11-dependent co-trafficking of TRAM from the recycling compartment to phagosomes via a direct SLAMF1-TRAM interaction.

    Evidence Knockdown/knockout in human macrophages, SLAMF1-TRAM co-IP with domain mapping, trafficking imaging, IFNβ and bacterial killing assays

    PMID:29440514

    Open questions at the time
    • Species-specificity (human vs mouse interaction) mechanism unexplained
    • How bacterial sensing triggers Rab11-dependent trafficking unclear
  16. 2020 Medium

    SLAMF1 was shown to be a target of bacterial immune evasion and a regulator of neutrophil autophagy, broadening its role as a sensor at the host-pathogen interface.

    Evidence Brucella Omp25-SLAMF1 binding with NF-κB/cytokine and mouse infection assays; Mtb-induced SLAMF1/LC3B co-localization and autophagy assays

    PMID:31953913 PMID:32954947

    Open questions at the time
    • Direct structural detail of Omp25-SLAMF1 contact not resolved
    • Single-lab observations

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the diverse SLAMF1 outputs—SAP/Fyn costimulation, TRAM trafficking, and the Beclin-1 autophagy complex—are coordinated or partitioned within a single cell remains unresolved.
  • No integrated structural model of the cytoplasmic tail engaging SAP versus Beclin-1 versus TRAM
  • Mechanism selecting between signaling and trafficking functions unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0001618 virus receptor activity 6 GO:0060089 molecular transducer activity 4 GO:0060090 molecular adaptor activity 3 GO:0098631 cell adhesion mediator activity 1
Localization
GO:0005886 plasma membrane 4 GO:0031410 cytoplasmic vesicle 3 GO:0005768 endosome 1
Pathway
R-HSA-168256 Immune System 5 R-HSA-162582 Signal Transduction 4 R-HSA-1643685 Disease 4 R-HSA-9612973 Autophagy 3 R-HSA-9609507 Protein localization 1
Partners
BECN1FYNLCKSH2D1ASHIPSHP-2SLAMF6TRAM
Complex memberships
Beclin-1/Vps34/UVRAG autophagy complex

Evidence

Reading pass · 32 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 SLAMF1 (SLAM/CD150) functions as a cellular receptor for measles virus, including wild-type clinical isolates and the Edmonston strain; transfection of human SLAM cDNA into non-susceptible cell lines confers measles virus binding, replication, and cytopathic effects. Transfection of human SLAM cDNA into non-susceptible cell lines followed by virus binding assay and replication assay Nature High 10972291
2001 CD150 associates with SH2-containing inositol phosphatase (SHIP) or SH2-containing protein tyrosine phosphatase (SHP-2) via phosphorylated tyrosines Y281 and Y327 in its cytoplasmic tail; the adaptor SH2D1A (SAP) regulates this association by facilitating SHIP binding and blocking SHP-2 binding, acting through a TxYxxV/I immunoreceptor tyrosine-based switch motif (ITSM). Co-immunoprecipitation, GST-fusion protein pulldown with tyrosine-to-phenylalanine mutants (Y269F, Y281F, Y307F, Y327F) Journal of immunology High 11313386
2001 CDw150/SLAM is an expression-cloned receptor for lymphotropic measles virus strains; both lymphotropic and laboratory measles virus strains bind human and marmoset CDw150 but show weak interaction with mouse CDw150; infection via CDw150 is independent of CD46. Expression cloning from B95-8 cDNA library, binding assay with soluble MV H protein, infection of CDw150-transfected CHO and HEK293T cells Virology High 11145884
2001 Measles virus hemagglutinin (H) protein, but not the fusion protein, is sufficient to downregulate SLAM (CD150) from the cell surface; downregulation can occur through intracellular interactions between H and SLAM in the ER, and also through receptor-mediated binding at the cell surface. Transfection of H protein expression plasmid, confocal microscopy, flow cytometry for surface SLAM levels Archives of virology Medium 11855632
2002 CD150 co-localizes with the TCR following CD3 triggering and is rapidly and reversibly tyrosine-phosphorylated upon TCR cross-linking; Src-like kinases Lck and Fyn phosphorylate tyrosine residues in the cytoplasmic tail of CD150. SAP binds CD150 via two distinct modes: phosphotyrosine-independent binding to Thr-Ile-Y281-Ala-Gln-Val and phosphotyrosine-dependent binding to Thr-Val-Y327-Ala-Ser-Val; a leucine residue L278 further stabilizes non-phospho binding at Y281. SAP blocks SHP-2 binding primarily at Y281. Confocal microscopy (co-localization), in vitro kinase assay, co-immunoprecipitation, site-directed mutagenesis Blood High 11806999
2002 SLAM (CD150) is a cellular receptor common to all 28 measles virus strains tested; a single amino acid exchange in the hemagglutinin at position 481 (Asn/Tyr, H481NY) determines whether virus can additionally utilize CD46, demonstrating that the binding sites for SLAM and CD46 on hemagglutinin are distinct. Infection of CHO cells expressing recombinant CD46 or SLAM with a panel of MV strains; recombinant virus with site-directed mutations The Journal of general virology High 12029158
2002 Measles virus can infect SLAM-negative cells at 2–3 log lower efficiency through an unidentified receptor distinct from both SLAM and CD46; this entry is blocked by anti-MV hemagglutinin antibody or fusion block peptide but not by anti-CD46 antibody, and occurs under conditions that inhibit endocytosis. Recombinant EGFP-expressing MV infection of SLAM-negative cell lines, antibody blocking assays, endocytosis inhibition Journal of virology Medium 12050387
2004 SLAMF1 (SLAM) knockout macrophages show defective production of IL-12, TNF, and nitric oxide in response to LPS but normal phagocytosis and normal responses to peptidoglycan or CpG; SLAM acts as a co-receptor regulating signals downstream of TLR4. SLAM-deficient CD4+ T cells show reduced TCR-induced IL-4 secretion with only slight upregulation of IFN-γ. SLAM-/- C57Bl/6 mice fail to clear Leishmania major due to defective macrophage function. Targeted gene knockout, in vitro cytokine assays, in vivo infection model (Leishmania major) The Journal of experimental medicine High 15123745
2004 SAP is required for phosphorylation of SLAM in thymocytes and peripheral T cells; SAP binds directly to both the SH3 domain and kinase domain of FynT, and addition of SAP to autoinhibited FynT causes a large increase in FynT catalytic activity; a SAP mutant (R78E) that cannot bind FynT SH3 domain neither activates FynT nor functions as an adaptor in T cells. In vitro kinase assay, yeast two-hybrid, GST pulldown, transfection with mutant constructs International immunology High 15096483
2004 Measles virus hemagglutinin (H) protein interacts with SLAM in the endoplasmic reticulum, promoting SLAM downregulation from the host cell surface; surface interactions between H and SLAM also contribute. H expression alone is sufficient for this downregulation. Plasmid transfection, co-culture experiments, flow cytometry, ER co-localization studies Journal of virology Medium 15331699
2005 SLAM (CD150) V domain is necessary and sufficient for measles virus receptor function; murine SLAM differs from human SLAM in receptor function due to residues at positions 60, 61, and 63, with histidine 61 being most critical; exchange of the region at amino acids 58–67 allows mouse SLAM to function as an MV receptor. Human/mouse chimeric SLAM constructs assessed by VSV pseudotype assay with site-directed mutagenesis The Journal of general virology High 12917459
2007 Homotypic interactions mediated by SLAMF1 and SLAMF6 (Ly108) on cortical thymocytes generate co-stimulatory signals during NKT cell selection; these signals involve downstream recruitment of SAP and Fyn kinase and are required for NKT lineage expansion and differentiation. These interactions only occur when selecting ligands (CD1d) are presented by thymocytes, not by epithelial cells, which do not express Slamf1/Slamf6. Genetic knockout models, epistasis analysis, in vivo NKT development assays Immunity High 18031695
2008 Measles virus hemagglutinin residue isoleucine 194 is essential for primary SLAM binding (as measured by surface plasmon resonance); a quartet of residues on propeller blade 5 is required for SLAM-dependent membrane fusion after binding but not for initial binding, indicating receptor-specific conformational changes post-binding trigger fusion protein unfolding. Surface plasmon resonance, receptor-specific fusion assays, crystal structure localization, site-directed mutagenesis The Journal of biological chemistry High 18292085
2010 SLAMF1 (CD150) signaling via SAP is specifically required for IL-4 production by germinal center T follicular helper (GC TFH) cells; SAP-deficient TFH cells lack GC TFH cells and are defective in IL-4 and IL-21 production; SLAM receptor ligation is required for this IL-4 production but not for TFH or GC TFH cell differentiation. SAP-deficient and SLAM-deficient mouse models, in vivo immunization, FACS-based identification of GC TFH cells, intracellular cytokine staining Journal of immunology High 20525889
2012 In E. coli-containing phagosomes of macrophages, SLAMF1 recruits a Beclin-1/Vps34/UVRAG protein complex; this complex regulates NOX2 (NADPH oxidase 2) activity and phagolysosomal maturation. The interaction requires the cytoplasmic tail of Slamf1 but not its ITAM motifs. Beclin-1 BD and CCD domains are required for binding to Slamf1. Slamf1 does not interact with Atg14L or Rubicon. Co-immunoprecipitation, in vitro pulldown, NOX2 activity assay in Beclin-1+/- macrophages, domain deletion mutagenesis, transfected HEK293 cells The Journal of biological chemistry High 22493499
2012 In the absence of SAP, SLAM family receptors Ly108 and 2B4 recruit increased levels of SHP-1 phosphatase, causing altered SHP-1 localization and decreased Src kinase activation at the immunological synapse. SAP-deficient CD8+ T cells show specific defects in synapse organization with B cell and low-avidity T cell targets (inefficient actin clearance), resulting in impaired cytotoxicity against these targets but not fibrosarcoma targets. SAP-deficient mouse T cells, immunological synapse imaging, phosphatase recruitment assay, cytotoxicity assays Immunity High 22683123
2015 SLAMF1 ligation with an agonistic antibody induces ROS accumulation, phosphorylation of p38, JNK1/2, and BCL2, and promotes autophagic flux in CLL cells. Beclin-1 dissociates from BCL2 upon SLAMF1 ligation, forming an autophagy macrocomplex containing SLAMF1, beclin-1, and VPS34. SLAMF1 silencing in CLL cells increases CXCR4, CD38, and CD44 expression and enhances chemotactic responses to CXCL12; SLAMF1-low cells are resistant to autophagy-activating agents. SLAMF1 siRNA knockdown, agonistic antibody ligation, co-immunoprecipitation (SLAMF1/beclin-1/VPS34 complex), ROS assay, autophagic flux assay, transwell chemotaxis assay The Journal of clinical investigation High 26619119
2015 SLAMF1 and SLAMF8 oppositely regulate in vivo migration of myeloid cells during inflammation: Slamf1-/- dendritic cells and macrophages show reduced migration in vivo and in vitro, whereas Slamf8-/- cells show accelerated migration. These effects are cell-intrinsic. Inhibition of ROS production in Slamf8-/- macrophages blocks their enhanced migration, linking SLAMF1/SLAMF8-mediated ROS regulation to myeloid cell migration. Slamf1-/- and Slamf8-/- mouse models, in vivo migration assays (skin sensitization, peritonitis, intestinal repopulation), transwell migration assay, ROS inhibitor (DPI) PloS one Medium 25799045
2017 SLAMF1 engagement by measles virus (MeV) induces macropinocytosis-like endocytic uptake of viral particles dependent on actin cytoskeletal rearrangement, membrane blebbing, and the RhoA-ROCK-myosin II signaling axis; this endocytic entry pathway is specific to SLAMF1-positive cells and occurs within 60 minutes of viral attachment. Live-cell imaging of viral particle colocalization with blebs, chemical inhibition of macropinocytosis/actin dynamics/ROCK-myosin II, infection assay in SLAMF1+ vs SLAMF1- cells Journal of virology Medium 28100610
2018 SLAMF1 is required for TLR4-mediated TRAM-TRIF-dependent signaling (IFNβ induction) and killing of Gram-negative bacteria by human macrophages. In resting macrophages, SLAMF1 localizes to the endocytic recycling compartment (ERC) and is trafficked together with TRAM to E. coli phagosomes in a Rab11-dependent manner upon bacterial stimulation. Endogenous SLAMF1 interacts with TRAM via amino acids 68–95 of TRAM and the 15 C-terminal amino acids of SLAMF1; this interaction is observed for human but not mouse proteins. SLAMF1 knockdown/knockout in human macrophages, co-immunoprecipitation (SLAMF1-TRAM), confocal microscopy of ERC-to-phagosome trafficking, domain deletion constructs, IFNβ induction assay, bacterial killing assay The Journal of cell biology High 29440514
2019 SLAM family receptors (SFRs), including SLAMF1, promote iNKT cell development by reducing TCR signal strength after positive selection; SFR deficiency upregulates inhibitory receptors (PD-1) to partially compensate; SLAMF6 alone can mimic SFR function and this involves the SAP-Fyn complex and phosphatase SHP-1. SFR-deficient mouse models, SLAMF6 single-receptor rescue experiments, epistasis with SAP/Fyn/SHP-1, iNKT cell development quantification, TCR signal strength assays Nature immunology High 30833791
2020 Brucella abortus outer membrane protein Omp25 specifically binds SLAMF1 in vitro; Omp25-dependent SLAMF1 engagement limits NF-κB translocation in dendritic cells, decreases pro-inflammatory cytokine secretion, impairs DC activation, and promotes bacterial persistence at the chronic stage of infection in vivo. In vitro binding assay (Omp25-SLAMF1), NF-κB translocation assay, cytokine measurement, mouse infection model Cellular microbiology Medium 31953913
2020 Human neutrophils upregulate SLAMF1 expression upon Mycobacterium tuberculosis (Mtb) stimulation; SLAMF1 co-localizes with LC3B+ vesicles, and SLAMF1 activation increases neutrophil autophagy induced by Mtb; tuberculosis patients' neutrophils show reduced SLAMF1 levels and lower autophagy. Flow cytometry for SLAMF1 expression, confocal microscopy (SLAMF1/LC3B co-localization), SLAMF1 agonistic antibody activation, autophagy flux assay Autophagy Medium 32954947
1993 IPO-3 (later identified as CD150/SLAMF1) is a novel heavily N-glycosylated phosphoglycoprotein (~75-95 kDa, 42 kDa protein core) expressed on activated B cells and some T cells; it has an associated protein kinase activity maintained in detergent lysates; cross-linking IPO-3 on B cells triggers increases in intracellular Ca2+ and augments IL-4/anti-CD40-driven proliferation. Monoclonal antibody characterization, in vitro kinase assay, calcium flux assay, proliferation assay Journal of immunology Medium 8409422
2002 CD150 (SLAMF1)-mediated T cell DNA synthesis (proliferation) induced by anti-CD150 antibody does not depend on SAP/SH2D1A, as similar levels are observed in SAP-/- T cells; however, SAP-/- T cells show higher IFN-γ production than WT upon anti-CD150 stimulation. Anti-CD150 cross-linking on CD4 T cells induces rapid serine phosphorylation of Akt/PKB. SAP-/- mouse T cells, anti-CD150 antibody stimulation, DNA synthesis assay, IFN-γ ELISA, Akt phosphorylation assay Blood Medium 12351401
2004 CD150 (SLAMF1)-induced ERK pathway activation in B cells requires SHIP but not SH2D1A (SAP); CD150-mediated Akt phosphorylation requires Syk and SH2D1A and is negatively regulated by Lyn and Btk; Lyn directly phosphorylates Y327 in CD150; the Akt pathway does not depend on CD150 tyrosine phosphorylation or CD150-SHP-2 association. DT40 B-cell sublines deficient in SHIP, SAP, Syk, Lyn, or Btk; CD150 ligation, Western blotting for ERK/Akt phosphorylation Blood High 15315965
2001 SLAM (CD150) is downregulated from the surface of activated peripheral blood lymphocytes and cell lines after measles virus infection or surface contact with MV envelope proteins; blocking SLAM or CD46 prevents virus binding but does not interfere with contact-mediated proliferation inhibition, indicating these are separable functions. Flow cytometry for SLAM surface expression, antibody blocking assays, proliferation assays Journal of virology Medium 11312320
2001 SLAM (CD150) is inducible on monocytes (which are SLAM-negative at rest) after stimulation with PHA, LPS, or MV; anti-SLAM monoclonal antibodies efficiently block MV infection of activated monocytes with a wild-type strain. Flow cytometry for SLAM induction on monocytes, blocking antibody assay for MV infection The Journal of general virology Medium 11714966
2006 CDV unable to recognize SLAM (CD150) does not spread in ferrets, formally proving that SLAM recognition is necessary for morbillivirus virulence and for lymphocyte-based dissemination into mucosal tissue and lymphatic organs. Recombinant CDV with receptor-ablating mutations in ferret infection model, GFP-reporter virus tracking Journal of virology High 16731947
2010 A recombinant measles virus with a single R533A substitution in hemagglutinin that is selectively unable to recognize SLAM (SLAM-blind) infects primary lymphocytes at low levels regardless of SLAM expression and causes no viremia or clinical symptoms in rhesus monkeys, formally proving that efficient SLAM recognition is necessary for MV virulence and pathogenesis. Site-directed mutagenesis of MV hemagglutinin, ex vivo lymphocyte infection assay, rhesus monkey intranasally inoculation model Journal of virology High 20071568
2001 SLAM (CD150) augments TCR-mediated cytotoxicity in both CD4+ and CD8+ T cells; SLAM engagement alone triggers cytotoxicity in herpesvirus saimiri-transformed T cells via lytic granule release, requiring extracellular Ca2+, cytoskeletal rearrangements, and MEK1/2 signaling, independent of CD95. Antibody crosslinking of SLAM on primary and transformed T cells, cytotoxicity assay, pharmacological inhibitors (MEK1/2, cytoskeletal), Ca2+ chelation European journal of immunology Medium 11536173
2012 Slamf1-/- mice are completely protected from acute lethal Trypanosoma cruzi challenge; Slamf1-deficient myeloid cells are impaired in intracellular T. cruzi replication and show altered cytokine production; IFN-γ production is reduced in the heart of Slamf1-/- mice despite comparable immune cell infiltration. Slamf1-/- mouse infection model, in vitro parasite replication assay in myeloid cells, cytokine measurement, anti-Slamf1 monoclonal antibody treatment PLoS pathogens Medium 22807679

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell 2455 15989959
2000 SLAM (CDw150) is a cellular receptor for measles virus. Nature 867 10972291
2011 SLAM family receptors and SAP adaptors in immunity. Annual review of immunology 405 21219180
2010 Germinal center T follicular helper cell IL-4 production is dependent on signaling lymphocytic activation molecule receptor (CD150). Journal of immunology (Baltimore, Md. : 1950) 355 20525889
2007 Homotypic interactions mediated by Slamf1 and Slamf6 receptors control NKT cell lineage development. Immunity 291 18031695
2018 SLAM-seq defines direct gene-regulatory functions of the BRD4-MYC axis. Science (New York, N.Y.) 289 29622725
2003 The SAP and SLAM families in immune responses and X-linked lymphoproliferative disease. Nature reviews. Immunology 233 14523387
2007 Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques. PLoS pathogens 215 18020706
2007 Regulation of cellular and humoral immune responses by the SLAM and SAP families of molecules. Annual review of immunology 210 17201683
2018 CD150high Bone Marrow Tregs Maintain Hematopoietic Stem Cell Quiescence and Immune Privilege via Adenosine. Cell stem cell 197 29456159
2002 SLAM (CD150)-independent measles virus entry as revealed by recombinant virus expressing green fluorescent protein. Journal of virology 181 12050387
2003 The dual-function CD150 receptor subfamily: the viral attraction. Nature immunology 180 12496974
2006 Immune regulation by SLAM family receptors and SAP-related adaptors. Nature reviews. Immunology 178 16493427
2009 SLAM receptors and SAP influence lymphocyte interactions, development and function. Nature reviews. Immunology 175 19079134
2001 CD150 association with either the SH2-containing inositol phosphatase or the SH2-containing protein tyrosine phosphatase is regulated by the adaptor protein SH2D1A. Journal of immunology (Baltimore, Md. : 1950) 170 11313386
2001 CD150 (SLAM) is a receptor for measles virus but is not involved in viral contact-mediated proliferation inhibition. Journal of virology 160 11312320
2001 CDw150(SLAM) is a receptor for a lymphotropic strain of measles virus and may account for the immunosuppressive properties of this virus. Virology 150 11145884
2004 The cell surface receptor SLAM controls T cell and macrophage functions. The Journal of experimental medicine 148 15123745
2008 The SLAM and SAP gene families control innate and adaptive immune responses. Advances in immunology 143 18501771
2015 SLAM family receptors in normal immunity and immune pathologies. Current opinion in immunology 126 26682762
2006 Receptor (SLAM [CD150]) recognition and the V protein sustain swift lymphocyte-based invasion of mucosal tissue and lymphatic organs by a morbillivirus. Journal of virology 123 16731947
2010 SLAM-family receptors: immune regulators with or without SAP-family adaptors. Cold Spring Harbor perspectives in biology 119 20300214
2010 SLAM family receptors and the SLAM-associated protein (SAP) modulate T cell functions. Seminars in immunopathology 102 20146065
2013 CS1, a SLAM family receptor involved in immune regulation, is a therapeutic target in multiple myeloma. Critical reviews in oncology/hematology 100 23731618
2007 Consequence of the SLAM-SAP signaling pathway in innate-like and conventional lymphocytes. Immunity 100 18031694
1993 Characterization of a cell surface glycoprotein IPO-3, expressed on activated human B and T lymphocytes. Journal of immunology (Baltimore, Md. : 1950) 99 8409422
2018 Dissecting newly transcribed and old RNA using GRAND-SLAM. Bioinformatics (Oxford, England) 95 29949974
2007 CD150- side population cells represent a functionally distinct population of long-term hematopoietic stem cells. Blood 95 18055867
2012 Positive and negative signaling through SLAM receptors regulate synapse organization and thresholds of cytolysis. Immunity 93 22683123
2003 The SLAM family of immune-cell receptors. Current opinion in immunology 90 12787752
2009 Measles virus infection of alveolar macrophages and dendritic cells precedes spread to lymphatic organs in transgenic mice expressing human signaling lymphocytic activation molecule (SLAM, CD150). Journal of virology 87 20042501
2006 NK cell regulation by SLAM family receptors and SAP-related adapters. Immunological reviews 84 17100873
1997 SLAM and its role in T cell activation and Th cell responses. Immunology and cell biology 84 9107577
2002 Analysis of receptor (CD46, CD150) usage by measles virus. The Journal of general virology 78 12029158
2004 Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4). Tissue antigens 73 15245368
2018 The SLAM family receptors: Potential therapeutic targets for inflammatory and autoimmune diseases. Autoimmunity reviews 71 29729453
2010 Measles virus selectively blind to signaling lymphocytic activation molecule (SLAM; CD150) is attenuated and induces strong adaptive immune responses in rhesus monkeys. Journal of virology 68 20071568
2007 Slamf1, the NKT cell control gene Nkt1. Journal of immunology (Baltimore, Md. : 1950) 65 17237411
2005 Cutting edge: the SLAM family receptor Ly108 controls T cell and neutrophil functions. Journal of immunology (Baltimore, Md. : 1950) 65 15879084
2001 Induction of the measles virus receptor SLAM (CD150) on monocytes. The Journal of general virology 64 11714966
2004 Measles virus receptors: SLAM and CD46. Reviews in medical virology 63 15248250
2010 Expression of SLAM (CD150) cell-surface receptors on human B-cell subsets: from pro-B to plasma cells. Immunology letters 62 20933013
2002 Molecular dissection of the signaling and costimulatory functions of CD150 (SLAM): CD150/SAP binding and CD150-mediated costimulation. Blood 62 11806999
2011 Increased expression of SLAM receptors SLAMF3 and SLAMF6 in systemic lupus erythematosus T lymphocytes promotes Th17 differentiation. Journal of immunology (Baltimore, Md. : 1950) 59 22184727
2002 The role of SAP in murine CD150 (SLAM)-mediated T-cell proliferation and interferon gamma production. Blood 58 12351401
2019 SLAM receptors foster iNKT cell development by reducing TCR signal strength after positive selection. Nature immunology 57 30833791
2008 Dynamic interaction of the measles virus hemagglutinin with its receptor signaling lymphocytic activation molecule (SLAM, CD150). The Journal of biological chemistry 57 18292085
2003 CD46- and CD150-independent endothelial cell infection with wild-type measles viruses. The Journal of general virology 55 12692284
2018 SLAMF1 is required for TLR4-mediated TRAM-TRIF-dependent signaling in human macrophages. The Journal of cell biology 54 29440514
2002 The morbillivirus receptor SLAM (CD150). Microbiology and immunology 54 12008921
2006 Measles virus infection of SLAM (CD150) knockin mice reproduces tropism and immunosuppression in human infection. Journal of virology 52 17135325
2023 SLAM-family receptors come of age as a potential molecular target in cancer immunotherapy. Frontiers in immunology 51 37251372
2005 Measles virus replication in lymphatic cells and organs of CD150 (SLAM) transgenic mice. Proceedings of the National Academy of Sciences of the United States of America 51 16260741
2004 SAP increases FynT kinase activity and is required for phosphorylation of SLAM and Ly9. International immunology 51 15096483
2005 Wild-type Rinderpest virus uses SLAM (CD150) as its receptor. The Journal of general virology 50 15914854
2015 SLAMF1 regulation of chemotaxis and autophagy determines CLL patient response. The Journal of clinical investigation 48 26619119
2006 A signal adaptor SLAM-associated protein regulates spontaneous autoimmunity and Fas-dependent lymphoproliferation in MRL-Faslpr lupus mice. Journal of immunology (Baltimore, Md. : 1950) 48 16365433
2001 CD150 is a member of a family of genes that encode glycoproteins on the surface of hematopoietic cells. Immunogenetics 48 11486275
2017 Dissection of SAP-dependent and SAP-independent SLAM family signaling in NKT cell development and humoral immunity. The Journal of experimental medicine 44 28049627
2003 Histidine at position 61 and its adjacent amino acid residues are critical for the ability of SLAM (CD150) to act as a cellular receptor for measles virus. The Journal of general virology 41 12917459
2015 Migration of myeloid cells during inflammation is differentially regulated by the cell surface receptors Slamf1 and Slamf8. PloS one 40 25799045
2001 Signaling lymphocytic activation molecule (SLAM) regulates T cellular cytotoxicity. European journal of immunology 39 11536173
2012 Receptor signaling lymphocyte-activation molecule family 1 (Slamf1) regulates membrane fusion and NADPH oxidase 2 (NOX2) activity by recruiting a Beclin-1/Vps34/ultraviolet radiation resistance-associated gene (UVRAG) complex. The Journal of biological chemistry 37 22493499
2006 High pathogenicity of wild-type measles virus infection in CD150 (SLAM) transgenic mice. Journal of virology 37 16775330
2017 The Measles Virus Receptor SLAMF1 Can Mediate Particle Endocytosis. Journal of virology 36 28100610
2008 Differential expression of CD150 (SLAM) family receptors by human hematopoietic stem and progenitor cells. Experimental hematology 36 18495325
2015 SLAM- and nectin-4-independent noncytolytic spread of canine distemper virus in astrocytes. Journal of virology 35 25787275
2010 The role of SLAM/CD2 polymorphisms in systemic autoimmunity. Current opinion in immunology 35 21094032
2004 Mechanism of CD150 (SLAM) down regulation from the host cell surface by measles virus hemagglutinin protein. Journal of virology 35 15331699
2021 SLAM Associated Protein Signaling in T Cells: Tilting the Balance Toward Autoimmunity. Frontiers in immunology 34 33936082
2018 SLAM-ITseq: sequencing cell type-specific transcriptomes without cell sorting. Development (Cambridge, England) 33 29945865
2013 Signaling lymphocytic activation molecule (SLAM)/SLAM-associated protein pathway regulates human B-cell tolerance. The Journal of allergy and clinical immunology 32 24373350
2020 Neutrophil autophagy during human active tuberculosis is modulated by SLAMF1. Autophagy 31 32954947
2004 The adaptor protein SH2D1A regulates signaling through CD150 (SLAM) in B cells. Blood 31 15315965
2006 The role of SAP and the SLAM family in autoimmunity. Current opinion in immunology 30 17011767
2006 SLAM family receptors and SAP-related adaptors: matters arising. Trends in immunology 29 16584920
2010 The role of SAP and SLAM family molecules in the humoral immune response. Annals of the New York Academy of Sciences 28 21091715
2012 The receptor Slamf1 on the surface of myeloid lineage cells controls susceptibility to infection by Trypanosoma cruzi. PLoS pathogens 27 22807679
2005 Fine-tuning of immune responses by SLAM-related receptors. Journal of leukocyte biology 27 16365151
2014 Fine specificity and molecular competition in SLAM family receptor signalling. PloS one 26 24642916
2018 SLAM family receptors in natural killer cells - Mediators of adhesion, activation and inhibition via cis and trans interactions. Clinical immunology (Orlando, Fla.) 25 30359773
2015 Canine Distemper Virus Fusion Activation: Critical Role of Residue E123 of CD150/SLAM. Journal of virology 25 26608324
2002 The measles virus hemagglutinin downregulates the cellular receptor SLAM (CD150). Archives of virology 25 11855632
2002 Mouse novel Ly9: a new member of the expanding CD150 (SLAM) family of leukocyte cell-surface receptors. Immunogenetics 25 12242590
2014 Role of SLAM family receptors and specific adapter SAP in innate-like lymphocytes. Critical reviews in immunology 24 24941157
2011 SLAM and DC-SIGN measles receptor polymorphisms and their impact on antibody and cytokine responses to measles vaccine. Vaccine 24 21645571
2020 Omp25-dependent engagement of SLAMF1 by Brucella abortus in dendritic cells limits acute inflammation and favours bacterial persistence in vivo. Cellular microbiology 23 31953913
2024 SLAM-ITseq identifies that Nrf2 induces liver regeneration through the pentose phosphate pathway. Developmental cell 22 38366599
2018 SLAMF1/CD150 in hematologic malignancies: Silent marker or active player? Clinical immunology (Orlando, Fla.) 22 30616923
2007 Immunohistochemical demonstration of the putative canine distemper virus receptor CD150 in dogs with and without distemper. Veterinary pathology 22 18039911
2019 NK cell recognition of hematopoietic cells by SLAM-SAP families. Cellular & molecular immunology 21 30911116
2018 CD150high CD4 T cells and CD150high regulatory T cells regulate hematopoietic stem cell quiescence via CD73. Haematologica 21 30545927
2011 Auto-antibody production and glomerulonephritis in congenic Slamf1-/- and Slamf2-/- [B6.129] but not in Slamf1-/- and Slamf2-/- [BALB/c.129] mice. International immunology 21 21278219
2020 Increased Plasma Levels of the Co-stimulatory Proteins CDCP1 and SLAMF1 in Patients With Autoimmune Endocrine Diseases. Frontiers in immunology 20 32983115
2011 A two-gene signature, SKI and SLAMF1, predicts time-to-treatment in previously untreated patients with chronic lymphocytic leukemia. PloS one 20 22194822
2023 SLAM-Drop-seq reveals mRNA kinetic rates throughout the cell cycle. Molecular systems biology 19 38778223
2020 SLAM family member 8 is expressed in and enhances the growth of anaplastic large cell lymphoma. Scientific reports 19 32054954
2019 Sequencing cell-type-specific transcriptomes with SLAM-ITseq. Nature protocols 19 31243395
2006 The role of SLAM family receptors in immune cell signaling. Biochemistry and cell biology = Biochimie et biologie cellulaire 19 17215871
2007 Enhancement of anti-tumor activity in vitro and in vivo by CD150 and SAP. Molecular immunology 18 17692919

Missed literature

Know a paper Affinage missed for SLAMF1? Flag it for the maintainers and the community.

No submissions yet.