Affinage

FYN

Tyrosine-protein kinase Fyn · UniProt P06241

Round 2 corrected
Length
537 aa
Mass
60.8 kDa
Annotated
2026-04-28
130 papers in source corpus 64 papers cited in narrative 64 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

FYN is a non-receptor Src-family tyrosine kinase that functions as a signaling hub at lipid raft membrane microdomains, coupling extracellular cues from integrins, growth factor receptors, GPI-anchored proteins, and cell-adhesion molecules to diverse downstream phosphorylation cascades that control synaptic plasticity, CNS myelination, immune cell activation, cell division, and mechanotransduction (PMID:9741627, PMID:1361685, PMID:11245687, PMID:11093170, PMID:16597701). Dually acylated by N-myristoylation and palmitoylation, FYN is concentrated in caveolae and detergent-resistant membrane domains where scaffold proteins (RACK1, caveolin-1, SHROOM3) position it adjacent to substrates including NR2B (Tyr-1472), nephrin, β-catenin (Tyr-142), NOX4 (Tyr-566), flotillin-1/2, and TOPK (Tyr-272); its catalytic activity is tuned by activating Tyr-420 and inhibitory Tyr-531 phosphorylation, with the balance set by upstream signals (integrin/contactin) and phosphatases (STEP61, PTPN23) (PMID:9839441, PMID:11024032, PMID:30341149, PMID:12668668, PMID:29555825, PMID:27525436, PMID:37016377, PMID:11983687, PMID:29066500, PMID:19625508). In the nervous system, FYN kinase activity is uniquely required for forebrain myelination via oligodendrocyte Tau–microtubule and hnRNP A2–MBP translation pathways, and for hippocampal LTP through NR2B phosphorylation scaffolded by RACK1 (PMID:11245687, PMID:11826099, PMID:18490510, PMID:12736333). FYN also serves as a convergence node in Alzheimer's disease pathogenesis, where Aβ oligomer–PrPC–mGluR5 complexes activate FYN to drive dendritic spine loss, Tau hyperphosphorylation, and synaptic dysfunction—phenotypes rescued by pharmacological FYN inhibition in APP/PS1 transgenic mice (PMID:24012003, PMID:25707991).

Mechanistic history

Synthesis pass · year-by-year structured walk · 17 steps
  1. 1986 High

    Identification of FYN as a distinct Src-family tyrosine kinase established a new signaling gene whose specific functions—versus overlapping roles with Src, Yes, and Lyn—remained undefined.

    Evidence Molecular cloning and sequencing of cDNA from a human library

    PMID:3526330

    Open questions at the time
    • No functional data; kinase activity not demonstrated
    • Expression pattern in tissues unknown beyond transcript size
  2. 1990 High

    Demonstration that FYN physically associates with the PDGF receptor upon ligand stimulation provided the first evidence that FYN is activated downstream of receptor tyrosine kinases, positioning it in growth-factor signaling.

    Evidence Co-immunoprecipitation and kinase activity assay from PDGF-stimulated fibroblasts

    PMID:1696179

    Open questions at the time
    • Substrates downstream of FYN in PDGF signaling unidentified
    • Whether FYN is directly phosphorylated by PDGFR not tested
  3. 1992 High

    Genetic knockout revealed a non-redundant requirement for FYN in hippocampal LTP and spatial learning, separating FYN's neural function from Src, Yes, and Abl and establishing it as a master regulator of synaptic plasticity.

    Evidence fyn-null mice, electrophysiology, Morris water maze

    PMID:1361685

    Open questions at the time
    • Direct substrates mediating LTP were unknown at this point
    • Mechanism linking FYN kinase activity to LTP induction not resolved
  4. 1998 High

    Characterization of FYN's dual acylation and caveolae targeting, together with the discovery that caveolin-1 scaffolds FYN to integrins for Shc/Ras-ERK activation, established the paradigm that lipid-raft compartmentalization is essential for FYN substrate selectivity and integrin-dependent signaling.

    Evidence Biochemical fractionation, acylation analysis, co-IP, SH3 binding, mutagenesis, Ras-ERK readout

    PMID:9741627 PMID:9839441

    Open questions at the time
    • How palmitoylation cycling regulates FYN trafficking was unresolved
    • Whether other scaffold proteins besides caveolin-1 recruit FYN in non-fibroblast contexts was unknown
  5. 1999 High

    Discovery that FYN resides in and is activated within oligodendrocyte lipid rafts via contactin/F3 and NCAM120 explained how glial surface receptors selectively engage FYN to initiate myelination programs.

    Evidence Detergent-resistant membrane fractionation, co-IP, kinase assay, antibody stimulation in oligodendrocytes

    PMID:10506155

    Open questions at the time
    • Downstream targets of FYN in myelination not yet identified
    • Whether FYN kinase activity per se (vs. adaptor function) was required not established
  6. 2001 High

    Kinase-dead knock-in mice showed that FYN catalytic activity—not merely its scaffolding—is uniquely required for forebrain myelination, and identification of NR2B Tyr-1472 as the principal FYN substrate in NMDA receptor complexes mechanistically linked FYN to excitatory synapse regulation.

    Evidence Kinase-dead knock-in and KO mice with myelin quantification and EM; in vitro kinase assay with mutagenesis, phospho-specific antibody, fyn-null mice, LTP electrophysiology

    PMID:11024032 PMID:11245687

    Open questions at the time
    • How FYN-dependent NR2B phosphorylation alters channel gating or trafficking not resolved
    • Whether NR2B Tyr-1472 phosphorylation is necessary and sufficient for LTP enhancement unknown
  7. 2002 High

    Identification of STEP61 as a phosphatase that selectively dephosphorylates FYN's activating Tyr-420 (but not inhibitory Tyr-531) revealed a postsynaptic brake on FYN activity, and discovery of the Fyn–Tau–tubulin cascade in oligodendrocytes connected FYN to microtubule-based process outgrowth.

    Evidence Substrate-trapping co-IP, in vitro phosphatase assay with site-specific mutants; co-IP with Tau deletion mutant, raft disruption in oligodendroglial cells

    PMID:11826099 PMID:11983687

    Open questions at the time
    • In vivo consequence of STEP61–FYN interaction on synaptic function not tested
    • Whether Tau is a direct FYN phosphorylation substrate or only a scaffolding partner was unclear
  8. 2003 High

    RACK1 was identified as a scaffold that holds FYN in complex with NR2B specifically in hippocampus, with ethanol-induced RACK1 dissociation unmasking FYN-mediated NR2B phosphorylation—providing a molecular mechanism for acute ethanol tolerance.

    Evidence Co-IP from hippocampal vs. cortical tissue, NR2B phosphorylation assay, ethanol treatment

    PMID:12736333

    Open questions at the time
    • Whether RACK1 regulation of FYN operates in non-ethanol contexts not fully explored
    • Structural basis for RACK1–FYN–NR2B tripartite complex unknown
  9. 2006 High

    Multiple studies converged to show FYN is a versatile integrator: it mediates fibronectin rigidity sensing via palmitoylation-dependent leading-edge recruitment, phosphorylates caveolin during coxsackievirus entry, controls Semaphorin3A-dependent dendritic spine maturation, and its crystal structure was solved, enabling rational inhibitor design.

    Evidence X-ray crystallography; palmitoylation mutant live-cell imaging; siRNA plus co-IP in viral entry; fyn-null neurons with Sema3A treatment

    PMID:16413486 PMID:16540575 PMID:16597701 PMID:16782058

    Open questions at the time
    • No selective FYN inhibitor achieved at this stage despite crystal structure
    • Structural basis for FYN substrate selectivity vs. other Src-family kinases remained elusive
  10. 2009 High

    Identification of flotillin-1 Tyr-160 and flotillin-2 Tyr-163 as direct FYN phosphorylation sites required for flotillin endocytosis, and integrin/contactin dual regulation of FYN Tyr-420/Tyr-531 balance, deepened understanding of how FYN coordinates membrane trafficking and myelination through site-specific phosphorylation events.

    Evidence SYF cell reconstitution, rapamycin-inducible dimerization, site-directed mutagenesis, endocytosis assays; siRNA plus phospho-specific western blot plus myelination co-culture

    PMID:19258392 PMID:19625508

    Open questions at the time
    • Whether flotillin phosphorylation by FYN occurs in neuronal contexts not shown
    • How integrin and contactin signals are integrated temporally on FYN in vivo was unresolved
  11. 2013 High

    Discovery that mGluR5 couples Aβ oligomer–PrPC complexes to cytoplasmic FYN activation established a complete receptor-to-kinase pathway for Alzheimer's synaptotoxicity, making FYN a therapeutic target in neurodegeneration.

    Evidence Co-IP, Xenopus oocyte calcium assay, neuronal calcium imaging, dendritic spine counting, mGluR5 antagonist rescue in AD transgenic mice

    PMID:24012003

    Open questions at the time
    • Whether FYN inhibition alone is sufficient for clinical benefit in human AD unknown
    • Mechanism by which FYN activation leads to Tau hyperphosphorylation not fully delineated
  12. 2015 High

    Pharmacological FYN inhibition with AZD0530 rescued spatial memory, synaptic loss, and Tau pathology in APP/PS1 mice without altering Aβ levels, providing preclinical validation of FYN as a druggable node downstream of Aβ in Alzheimer's disease.

    Evidence AZD0530 treatment in APP/PS1 transgenic mice, Morris water maze, novel object recognition, immunohistochemistry, downstream Pyk2/NR2B phosphorylation in brain slices

    PMID:25707991

    Open questions at the time
    • AZD0530 is not fully selective for FYN over other Src-family kinases
    • Clinical translation to human AD patients not addressed
  13. 2016 High

    Identification of NOX4 Tyr-566 as a direct FYN phosphorylation site that suppresses ROS production, with fyn-null mice showing exacerbated cardiac hypertrophy rescued by Nox4 deletion, revealed FYN as a cardioprotective kinase operating through redox control.

    Evidence Co-IP, in vitro phosphorylation, Y566 mutagenesis, fyn−/−/nox4−/− genetic epistasis mice, cardiac function measurements

    PMID:27525436

    Open questions at the time
    • Whether FYN–NOX4 axis operates in non-cardiac tissues not explored
    • Upstream signals activating FYN in cardiomyocytes incompletely defined
  14. 2017 High

    Genetic epistasis showing that PTPN23 loss hyperactivates FYN and drives β-catenin Tyr-142 phosphorylation in breast tumorigenesis—reversed by FYN CRISPR knockout—established FYN as a critical oncogenic effector restrained by a tumor-suppressor phosphatase.

    Evidence PTPN23/FYN CRISPR double KO, orthotopic transplantation, xenograft model, AZD0530 treatment

    PMID:29066500

    Open questions at the time
    • Structural basis for PTPN23–FYN interaction unknown
    • Whether other phosphatases cooperate with PTPN23 to restrain FYN in epithelia not tested
  15. 2018 High

    Direct demonstration that FYN phosphorylates β-catenin at Tyr-142 to stabilize it and promote nuclear translocation in chondrocytes, with fyn-null mice protected from osteoarthritis, expanded FYN's disease relevance beyond neurodegeneration to joint degeneration.

    Evidence In vitro kinase assay, Tyr-142 phosphorylation analysis, fyn-KO OA mouse model, Fyn inhibitor treatment

    PMID:29555825

    Open questions at the time
    • Whether FYN-mediated β-catenin activation contributes to cartilage homeostasis under non-pathological conditions is unclear
    • Contribution of other kinases to β-catenin Tyr-142 phosphorylation in chondrocytes not excluded
  16. 2019 High

    Single-molecule tracking showed that Tau controls FYN nanoscale organization in dendritic spines, with P301L mutant Tau immobilizing FYN—providing a biophysical mechanism for how tauopathy concentrates FYN at postsynaptic sites to exacerbate excitotoxicity.

    Evidence Single-molecule tracking in hippocampal neurons, Tau knockout/overexpression, P301L Tau expression

    PMID:31237563

    Open questions at the time
    • Whether immobilized FYN is catalytically hyperactive or merely mislocalized not directly measured
    • Applicability to human tauopathy neurons not tested
  17. 2023 High

    Identification of TOPK Tyr-272 as a direct FYN substrate driving gastric cancer proliferation and metastasis via HSPB1 phosphorylation further broadened FYN's oncogenic substrate repertoire and confirmed a kinase-substrate-effector cascade in solid tumors.

    Evidence Co-IP, 32P kinase assay, phospho-specific antibody, TOPK-Y272F mutagenesis, TOPK KO mice, phosphoproteomics

    PMID:37016377

    Open questions at the time
    • Whether FYN–TOPK axis operates in cancers beyond gastric cancer not explored
    • Selective FYN inhibitors for clinical oncology remain unavailable

Open questions

Synthesis pass · forward-looking unresolved questions
  • Despite extensive substrate identification, no selective FYN inhibitor distinguishing it from other Src-family kinases exists, and the structural determinants governing FYN's non-redundant substrate selectivity over Src/Yes/Lyn remain poorly understood at the atomic level.
  • No FYN-selective pharmacological inhibitor validated in vivo
  • Full phosphoproteome of FYN-dependent substrates not systematically catalogued
  • How FYN's unique N-terminal domain determines substrate specificity lacks structural explanation

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 8 GO:0140096 catalytic activity, acting on a protein 8 GO:0008092 cytoskeletal protein binding 2 GO:0008289 lipid binding 1
Localization
GO:0005886 plasma membrane 6 GO:0005856 cytoskeleton 3 GO:0005829 cytosol 2 GO:0031410 cytoplasmic vesicle 2
Pathway
R-HSA-162582 Signal Transduction 12 R-HSA-112316 Neuronal System 7 R-HSA-1266738 Developmental Biology 6 R-HSA-168256 Immune System 5 R-HSA-1474165 Reproduction 4 R-HSA-1500931 Cell-Cell communication 4 R-HSA-1643685 Disease 4 R-HSA-5653656 Vesicle-mediated transport 3
Partners
CAV1GNB2L1MAPTNPHS1NTRK2PTPN23PTPN5SHROOM3

Evidence

Reading pass · 64 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1986 FYN (originally named 'syn') was identified as a new member of the protein-tyrosine kinase family, closely related to v-yes and human c-src, encoded on human chromosome 6 with a distinct 2.8-kb transcript. Molecular cloning and nucleotide sequence analysis of cDNA Proceedings of the National Academy of Sciences of the United States of America High 3526330
1990 Fyn (p59fyn) is physically associated with the PDGF receptor upon PDGF stimulation; Fyn kinase activity is elevated after PDGF stimulation coincident with co-association with the activated receptor. Co-immunoprecipitation and kinase activity assay from PDGF-stimulated fibroblasts Cell High 1696179
1990 Xenopus laevis possesses fyn genes encoding a protein with 96% amino acid identity to human FYN; fyn transcripts are present in oocytes, implicating multiple Src-family kinases in oogenesis or early development. cDNA library screening, sequencing, and Northern blot analysis Oncogene Medium 2179818
1991 CD36 (glycoprotein IV) is physically associated with Fyn, Lyn, and Yes protein-tyrosine kinases in human platelets and in GPIV-expressing cell lines, suggesting GPIV ligand interactions activate tyrosine phosphorylation signaling pathways. Co-immunoprecipitation from platelet lysates and cell lines Proceedings of the National Academy of Sciences of the United States of America High 1715582
1992 Fyn is required for hippocampal long-term potentiation (LTP) and spatial learning; fyn mutant mice show blunted LTP, impaired spatial learning, and increased granule/pyramidal cell numbers in the hippocampus, while src, yes, and abl mutations had no LTP effect. Genetic knockout mice, electrophysiology (LTP induction), Morris water maze spatial learning Science High 1361685
1992 p21rasGAP associates with Fyn, Lyn, and Yes in thrombin-activated platelets, forming kinase-substrate complexes detectable only after agonist stimulation, positioning Fyn downstream of a G-protein-coupled receptor in platelet signaling. Kinase trapping strategy with immunoprecipitation and tyrosine phosphorylation analysis The Journal of biological chemistry Medium 1544885
1995 Fyn and Src have both specific and overlapping roles in cytoskeletal regulation: focal adhesion kinase and paxillin hyperphosphorylation is partly dependent on both Src and Fyn in csk-null cells, whereas cortactin and tensin hyperphosphorylation is Src-dependent. Fyn does not rescue the neurulation defect of csk-null mice. Genetic epistasis (double mutant analysis in csk-/- src-/- fyn-/- mice) with biochemical tyrosine phosphorylation analysis Nature High 7617039
1996 Fyn kinase localizes to the midspace of dividing pro-B cells at anaphase and regulates progression of cytokinesis; fyn-null pro-B cells arrest at late telophase under serum-free conditions, accumulating binucleated cells. fyn knockout mice, cell cycle analysis, immunolocalization, morphological analysis The Journal of cell biology Medium 8567733
1998 Fyn is N-myristoylated on cytosolic polysomes and then palmitoylated at the plasma membrane; dually acylated Fyn clusters in caveolae-like membrane microdomains and interacts with diverse signaling molecules. Biochemical fractionation and acylation analysis The international journal of biochemistry & cell biology Medium 9839441
1998 Caveolin-1 functions as a membrane adaptor linking integrin alpha subunits to Fyn; upon integrin ligation, Fyn is activated and binds via its SH3 domain to Shc, leading to Shc Tyr-317 phosphorylation and Grb2 recruitment, coupling integrins to the Ras-ERK pathway for anchorage-dependent cell growth. Co-immunoprecipitation, SH3 domain binding assay, mutagenesis, Ras-ERK pathway assay Cell High 9741627
1998 CD73-mediated signal transduction in murine splenic T cells requires Fyn; fyn-null mice are unresponsive to CD73 ligation, demonstrating Fyn as a necessary downstream kinase for CD73 signaling. fyn-null mice, proliferation assay, IL-2 secretion assay upon CD73 ligation European journal of immunology Medium 9808167
1999 Fyn kinase associates with and is maximally active in GPI-anchored protein-containing lipid rafts of oligodendrocytes; Fyn is tightly associated with NCAM 120 and F3 within rafts, and anti-F3 antibody stimulation activates Fyn specifically in rafts. Compartmentation in rafts is prerequisite for F3-Fyn association and kinase activation during myelination. Detergent-resistant membrane fractionation, co-immunoprecipitation, kinase activity assay, antibody stimulation The Journal of biological chemistry High 10506155
1999 Fyn associates with and dephosphorylates p59 Fyn-binding protein (FYB/ADAP) and Cas in macrophages; these are substrates of YopH (Yersinia phosphatase) whose dephosphorylation disrupts focal complexes and blocks phagocytosis. Substrate-trapping immunoprecipitation with inactive YopH, immunolocalization Microbial pathogenesis Medium 10502464
2000 Fyn (p59fyn) is an essential upstream kinase in the CD2 signaling pathway; fyn-null T cells show markedly reduced Ca2+ mobilization, MAPK activation, and proliferation in response to CD2 clustering. Fyn-dependent CD2 pathway components include PLC-γ1, Vav, PKC-θ, Dok, FAK, and Pyk2. CD2 transgenic fyn-null mice, calcium mobilization, MAPK assay, kinase activity assays, immunoprecipitation European journal of immunology High 11093170
2001 Fyn kinase activity (not just Fyn protein) is uniquely required for CNS myelination in the forebrain; a single amino acid substitution abolishing kinase activity causes a myelin deficit as severe as complete Fyn absence. Other Src-family kinases (Src, Yes, Lyn) do not compensate. fyn knockout mice, kinase-dead knock-in mice, myelin quantification, oligodendrocyte counting, EM The Journal of neuroscience High 11245687
2001 Fyn phosphorylates the NR2B (GluRε2) subunit of NMDA receptors at multiple tyrosines; Tyr-1472 is the major in vivo phosphorylation site, its phosphorylation is greatly reduced in fyn mutant mice, and it is enhanced after hippocampal LTP induction. In vitro kinase assay with mutagenesis, co-expression in HEK293, phospho-specific antibody, fyn mutant mice, LTP electrophysiology The Journal of biological chemistry High 11024032
2002 Fyn binds Tau via its SH3 domain and binds α-Tubulin via its SH2 and SH3 domains in oligodendrocytes; the Fyn-Tau-microtubule cascade is required for oligodendroglial process outgrowth. Disrupting the Fyn-Tau interaction reduces process number and length. Co-immunoprecipitation, dominant-negative competition with Tau deletion mutant, Fumonisin B1 raft disruption, transfection in oligodendroglial cells The Journal of neuroscience High 11826099
2002 STEP61, a membrane-associated phosphatase in postsynaptic densities, associates specifically with Fyn and dephosphorylates Fyn at Tyr-420 (activating site) but not Tyr-531, providing a mechanism for reducing Fyn activity in PSDs. STEP61 binds Fyn through its proline-rich and KIM domains; Fyn binds STEP61 through its SH2 domain and Fyn's unique N-terminal domain. Substrate-trapping co-transfection in HEK293, reciprocal co-immunoprecipitation, in vitro phosphatase assay with site-specific mutants The Journal of biological chemistry High 11983687
2003 Fyn directly binds Nephrin via its SH3 domain and phosphorylates Nephrin on tyrosine residues in podocyte detergent-resistant membranes; Fyn deletion in mice causes coarsening of podocyte foot processes and marked attenuation of Nephrin phosphorylation in glomerular detergent-resistant fractions. Co-immunoprecipitation, in vitro direct binding/phosphorylation assay, fyn/yes knockout mice, glomerular fractionation The Journal of biological chemistry High 12668668
2003 RACK1 scaffolds Fyn to the NR2B subunit of NMDA receptors specifically in the hippocampus but not cortex; acute ethanol dissociates RACK1 from the complex, facilitating Fyn-mediated NR2B phosphorylation and enhanced channel activity, accounting for hippocampus-selective acute tolerance to ethanol. Co-immunoprecipitation from hippocampal vs. cortical tissue, NR2B phosphorylation assay, ethanol treatment, RACK1 dissociation studies The Journal of neuroscience High 12736333
2003 In highly metastatic melanoma cells, Fyn is selectively activated among Src-family kinases; Fyn forms a stable complex with cortactin, phosphorylates it, and co-localizes with it at cell membranes, promoting cell migration. Fyn activation occurs downstream of integrin engagement. Kinase activity assay, co-immunoprecipitation, immunolocalization, migration assay, integrin stimulation The Journal of biological chemistry Medium 13129922
2003 p250GAP, a brain-enriched RhoGAP protein, associates with Fyn in vitro and in vivo and is tyrosine-phosphorylated by Fyn when co-expressed; this phosphorylation is enhanced during oligodendrocyte CG4 cell differentiation, suggesting Fyn regulates RhoGAP activity during oligodendrocyte maturation. Co-immunoprecipitation, in vitro kinase assay, co-expression in HEK293T, differentiation model Biochemical and biophysical research communications Medium 12788081
2004 Lck and Fyn are segregated into different lipid raft microdomains in unstimulated T cells; Lck activation is upstream of Fyn activation in a unidirectional lck-dependent manner, with their activations being temporally and spatially uncoupled during TCR/CD3 signaling. Membrane fractionation, kinase activity assays, T cell receptor stimulation experiments Molecular immunology Medium 15220001
2005 Combined absence of Src and Fyn almost abolishes tyrosine phosphorylation of Dab1 and causes a reeler-like phenotype (inverted cortical layering, impaired Purkinje plate), demonstrating that Src and Fyn are redundantly required for Reelin-dependent neuronal positioning in vivo. Double knockout mice (src-/-fyn-/-), Dab1 phosphorylation assay, histological brain analysis The Journal of neuroscience High 16162939
2006 Crystal structure of the human Fyn kinase domain complexed with staurosporine at 2.8 Å resolution; staurosporine occupies the ATP-binding site with small structural differences from Lck and Csk complexes that may enable selective inhibitor design. X-ray crystallography Biochemical and biophysical research communications High 16782058
2006 Fyn mediates Semaphorin3A-dependent dendritic spine maturation and PSD-95 cluster formation in cortical neurons; fyn-null neurons bear few dendritic spines, Sema3A does not induce PSD-95 clustering in fyn-null neurons, and double-heterozygous sema3A/fyn mice show reduced spine density. fyn-null mice, Sema3A treatment, immunostaining for PSD-95 and NRP-1, PP2 inhibitor, genetic interaction analysis The Journal of neuroscience High 16540575
2006 Virus (coxsackievirus) attachment to DAF activates Fyn kinase, which is required for phosphorylation of caveolin and transport of the virus into the cell within caveolar vesicles. A separate Abl/Rac pathway mediates actin rearrangements for viral movement to tight junctions. Kinase inhibition, siRNA knockdown, co-immunoprecipitation, viral entry assay, caveolin phosphorylation assay Cell High 16413486
2006 Fyn (but not Src) is recruited to early adhesions at the leading edge in response to fibronectin rigidity; this requires Fyn's palmitoylation site. Fyn-dependent phosphorylation of p130Cas at the leading edge mediates the cellular rigidity response and growth. Live-cell imaging, GFP fusion proteins, palmitoylation mutant, p130Cas phosphorylation assay, spreading/growth assays Molecular biology of the cell Medium 16597701
2006 Crystal structure of HIV-1 Nef conserved core complexed with a mutant Fyn SH3 domain (R96I) shows that the Nef PxxP motif forms a polyproline type II helix engaging the SH3 domain; this reveals how high-affinity and specificity is achieved in the Nef-SH3 interaction. X-ray crystallography of Nef–Fyn SH3 complex Cell High 8681387
2007 Oxidant-mediated Fyn kinase activation leads to secondary activation of the c-Cbl ubiquitin ligase, causing specific reduction of c-Cbl target PDGFRα and other RTKs (but not TrkC), thereby suppressing mitogenic signaling in CNS progenitor cells at environmentally relevant toxicant levels. In vitro and in vivo Fyn kinase activation assay, c-Cbl ubiquitin ligase activity, RTK level quantification, toxicant treatment PLoS biology Medium 17298174
2007 Unc119 interacts with and activates Fyn; Unc119 is required for TGF-β/PDGF-induced Fyn phosphorylation. Unc119-induced myofibroblast differentiation (α-SMA induction) requires Fyn and the p38 MAPK pathway; Unc119 overexpression does not stimulate α-SMA in SYF cells unless Fyn is restored. Co-immunoprecipitation, co-localization, kinase activity assay, SYF reconstitution, siRNA, p38 inhibition Journal of immunology Medium 17579091
2008 Activated Fyn phosphorylates hnRNP A2 in oligodendrocytes; L1 neuronal adhesion molecule binding activates Fyn, increasing hnRNP A2 phosphorylation and stimulating localized translation of MBP mRNA at axon-glia contact sites. Phosphorylation assay, L1-Fc stimulation, reporter construct translation assay, hnRNP A2 immunoprecipitation from oligodendrocytes The Journal of cell biology High 18490510
2008 Murine gammaherpesvirus M2 protein is phosphorylated by Fyn at Tyr-120 and Tyr-129 in non-hematopoietic cells; phospho-Tyr-120 creates docking sites for Vav1 and Fyn SH2 domains, enabling trimolecular Fyn/M2/Vav1 complex formation that activates Vav1/Rac1 pathway for B-cell latency establishment. In vivo phosphorylation analysis, SH2 domain binding assay, co-immunoprecipitation, viral mutant infection in mice PloS one Medium 18301737
2008 Fyn deficiency in zebrafish (along with Yes) causes convergent-extension cell movement defects; comparative phosphoproteomics of Fyn/Yes morphant embryos identifies adducin and PDLIM5 phosphorylation changes, implicating Fyn/Yes in regulating signaling pathways controlling gastrulation movements. Morpholino knockdown in zebrafish, quantitative phosphoproteomics with stable isotope labeling, LC-MS/MS Molecular & cellular proteomics Medium 18550893
2008 Fyn kinase is required for meiotic maturation in mouse oocytes; FYN siRNA knockdown and FYN-null oocytes show ~50% reduction in progression to metaphase-II, with meiotic spindle and chromosome alignment defects. siRNA knockdown, fyn-null mice, in vitro and in vivo oocyte maturation, SKI606 inhibitor treatment Developmental biology Medium 19118543
2009 Fyn specifically regulates endocytosis of flotillin-1 and flotillin-2 by directly phosphorylating Tyr-160 of flotillin-1 and Tyr-163 of flotillin-2; these phosphorylations are required for flotillin internalization and uptake of the GPI-linked protein CD59. SYF cell reconstitution with individual Src kinases, active Fyn overexpression, rapamycin-inducible Fyn dimerization system, site-directed mutagenesis, endocytosis assays Journal of cell science High 19258392
2009 An integrin/contactin complex on oligodendrocytes integrates signals by differential Fyn phosphorylation: integrin induces dephosphorylation of inhibitory Tyr-531, while contactin increases phosphorylation of both Tyr-531 and activating Tyr-420; combined effect enhances Fyn activity and regulates oligodendrocyte survival and myelination. siRNA knockdown, L1-Fc stimulation, phospho-specific western blot for Tyr-531 and Tyr-420, myelination co-culture assay The Journal of neuroscience High 19625508
2009 Laminin promotes oligodendrocyte differentiation via Fyn activation; in laminin-deficient brains, Fyn activity is suppressed and levels of negative regulators Csk and Cbp are elevated, indicating that laminin modulates Fyn activity via its regulatory proteins. fyn KO mice, laminin-deficient (dy/dy) mice, Src kinase activity assay, western blotting for Csk/Cbp The Journal of neuroscience Medium 19776266
2009 Srcasm promotes Fyn kinase down-regulation in a phosphorylation-dependent manner requiring Fyn kinase activity, Srcasm phosphorylation, and the Srcasm GAT domain, acting as a molecular rheostat for activated Fyn in keratinocytes. K14-Fyn transgenic mice, K14-Fyn/Srcasm double transgenic mice, biochemical kinase level analysis, phosphorylation-null mutant Cancer research Medium 19934324
2010 Fyn is recruited to the cortex overlying chromosomes and co-localizes with F-actin during meiotic and mitotic cleavage furrow ingression in mouse oocytes; dominant-negative Fyn prolongs furrow ingression duration and impairs polar body extrusion, implicating Fyn in actin cytoskeletal regulation during cell division. Live-cell confocal imaging, microinjection of Fyn cRNAs (wild-type, dominant-negative), SU6656 inhibitor treatment Reproduction Medium 20841362
2010 Fyn-null female mice exhibit reduced fertility; Fyn-null oocytes show higher frequency of spindle/chromosome abnormalities and 24% failure of pronuclear congression, with altered protein tyrosine phosphorylation patterns attributed to actin cytoskeletal defects rather than calcium signaling. fyn knockout mice, oocyte maturation assays, fertilization assays, phosphotyrosine western blot, morphological analysis Reproduction, fertility, and development Medium 20591331
2011 Fyn requires HnRNPA2B1 and Sam68 to coordinate apoptosis regulation in pancreatic cancer; Fyn activity regulates HnRNPA2B1 expression (affecting Bcl-xS formation) and phosphorylates Sam68 (affecting Bcl-xL formation), with the two pathways cooperating to control cell survival. Kinase-dead Fyn, RNAi knockdown, Bcl-x splicing assay, apoptosis assay, co-immunoprecipitation Carcinogenesis Medium 21642356
2011 Fyn regulates the Th17/Treg balance by controlling RORγt upregulation kinetics and STAT3 activation; fyn-null CD4+ T cells under Th17-skewing conditions show decreased IL-17, increased Foxp3, delayed Il23r and Irf4 upregulation, and aberrant Socs3 expression, reducing Th17 cells in vivo. fyn knockout mice, Th17 polarization cultures, intracellular cytokine staining, T cell transfer to Rag1-/- hosts Journal of immunology Medium 22539787
2012 Fyn is required for meiosis resumption in mouse oocytes; Fyn localizes to the oocyte cortex and spindle poles, is recruited to the contractile ring during polar body extrusion, and undergoes proteasome-independent degradation during GVBD. Dominant-negative Fyn or SU6656 inhibits GVBD and polar body extrusion. Immunostaining, live confocal imaging, dominant-negative microinjection, SU6656 inhibitor, western blot Cell cycle Medium 20372074
2012 Fyn kinase deficiency in male mice causes high frequency of abnormal sperm morphology and reduced acrosome reaction capacity; Fyn-null sperm have slightly modified capacitation-associated tyrosine phosphorylation, suggesting Fyn functions in acrosome shaping within the testis. fyn knockout mice, artificial insemination competition, morphological analysis, acrosome reaction assay, tyrosine phosphorylation western blot Biology of reproduction Medium 21918125
2013 mGluR5 is a co-receptor coupling Aβ oligomer-PrPC complexes to Fyn kinase activation; PrPC and mGluR5 interact physically, and cytoplasmic Fyn forms a complex with mGluR5. Aβo-PrPC-mGluR5 signaling activates Fyn, mediates eEF2 phosphorylation and dendritic spine loss. Co-immunoprecipitation, Xenopus oocyte calcium assay, neuronal calcium imaging, dendritic spine counting, mGluR5 antagonist rescue in AD transgenic mice Neuron High 24012003
2014 FYN directly phosphorylates COX2 at Tyr-446; a phospho-mimetic COX2 mutation (Y446E) promotes COX2 enzymatic activity while a phosphorylation-blocking mutation (Y446F) prevents FYN-mediated COX2 activation, establishing post-translational regulation of COX2 by FYN. Co-immunoprecipitation, in vitro kinase assay, phospho-mimetic and blocking mutagenesis, COX2 activity assay Oncotarget High 24970799
2014 FYN mutations are recurrently found in peripheral T cell lymphomas (PTCL), implicating Src signaling in PTCL pathogenesis. The mutations were identified by whole-exome sequencing and targeted deep sequencing. Whole-exome sequencing, RNA sequencing, targeted deep sequencing of tumor-normal pairs Nature genetics Low 24413734
2015 Fyn inhibition with AZD0530 prevents Aβo-induced Fyn signaling and downstream phosphorylation of Pyk2 and NR2B in brain slices; treatment of APP/PS1 transgenic mice rescues spatial memory deficits, synaptic depletion, microglial activation, and Tau phosphorylation abnormalities without altering APP/Aβ metabolism. Brain slice phosphorylation assays, Morris water maze, novel object recognition, immunohistochemistry, APP/PS1/Tau transgenic mice, AZD0530 pharmacological inhibition Annals of neurology High 25707991
2015 BDNF activates Fyn phosphorylation in oligodendrocytes via TrkB; Fyn acts as an intermediate kinase phosphorylating Erk1/2 downstream of BDNF-TrkB, promoting myelination. Kinase-dead Fyn abolishes BDNF-induced Erk1/2 activation and myelination, and Fyn overexpression mimics BDNF effects. Kinase-dead Fyn overexpression, PP2 inhibition, myelinating co-cultures, Erk1/2 phosphorylation assay, TrkB dependency analysis, in vivo colocalization Glia High 26449489
2015 Fyn-phosphorylated PIKE-A binds to the AMPK catalytic α subunit and impairs T172 phosphorylation, blocking AMPK kinase activity and its tumor-suppressive actions; depletion of Fyn or mutation of Fyn phosphorylation sites on PIKE-A abolishes PIKE-A/AMPK association. Co-immunoprecipitation, in vitro kinase assay, Fyn depletion by shRNA, pharmacological inhibition, AMPK T172 phosphorylation assay, oncogenic assays Cell death and differentiation Medium 26001218
2015 Thy-1 physically couples to inactive αvβ3 integrin via its RGD-like motif; this coupling facilitates membrane raft/Src family kinase pre-adhesion clustering. Loss of Thy-1 impairs Fyn recruitment to adhesion complexes, mechanosensitive Rho signaling, and rigidity sensing in fibroblasts. Co-immunoprecipitation, proximity ligation assay, Fyn recruitment imaging, Rho signaling assay, traction force microscopy The Journal of cell biology Medium 26459603
2016 FYN interacts with the C-terminal domain of NOX4 in cardiomyocytes; FYN directly phosphorylates NOX4 at Tyr-566, negatively regulating NOX4-induced superoxide production and apoptosis. FYN-deficient mice show exacerbated cardiac hypertrophy, dysfunction, and increased ROS; deletion of Nox4 rescues LV remodeling in FYN-deficient mice. Co-immunoprecipitation, in vitro phosphorylation assay, Y566 mutagenesis, fyn-/- and nox4-/- mice, cardiac function measurements, ROS assay The Journal of clinical investigation High 27525436
2016 Fyn-dependent phosphorylation of SHP-1 at serine-591 inactivates the phosphatase, enabling activatory immunoreceptor signaling; in contrast, Lyn phosphorylates SHP-1 Tyr-536 to activate it and promote inhibitory signaling. Fyn deficiency is protective in nephritis and arthritis mouse models. Phosphorylation site mutagenesis, fyn and lyn knockout mice, nephritis/arthritis mouse models, SHP-1 phosphatase activity assay Nature communications High 28811476
2016 MOBP synthesis in oligodendrocytes is stimulated by Fyn activity; MOBP promotes oligodendroglial morphological differentiation, identifying MOBP as a downstream effector of Fyn-regulated myelin protein synthesis. Fyn kinase inhibition/activation in cultured primary oligodendrocytes, MOBP knockdown, morphological analysis Journal of cell science Medium 26801084
2016 CD36 nanoclusters are enriched with Fyn at baseline; exposure to multivalent ligands (TSP-1 or anti-CD36 IgM) enhances CD36 clustering and activates Fyn without altering Fyn enrichment at nanoclusters, demonstrating that cluster compaction/growth is the activating signal. Quantitative multi-channel high- and super-resolution imaging, pharmacological perturbation, Fyn activation assay Journal of cell science Medium 27694211
2017 PTPN23 normally suppresses FYN activity in mammary epithelial cells; PTPN23 depletion causes hyperphosphorylation of FYN autophosphorylation site and Tyr-142 of β-catenin. Double knockout of FYN and PTPN23 attenuates tumor outgrowth, establishing PTPN23 as a key phosphatase suppressing FYN in breast tumorigenesis. PTPN23 knockdown, orthotopic transplantation mouse model, FYN/PTPN23 CRISPR/Cas9 double KO, xenograft model, AZD0530 treatment Genes & development High 29066500
2018 Fyn directly phosphorylates β-catenin at Tyr-142, stabilizing β-catenin and promoting its nuclear translocation in chondrocytes; Fyn-null mice are protected from post-traumatic and age-dependent osteoarthritis, and Fyn inhibitors block β-catenin pathway activation and ECM catabolic enzymes. In vitro kinase assay, Tyr-142 phosphorylation analysis, β-catenin nuclear translocation assay, fyn-knockout OA mouse model, Fyn inhibitor treatment Annals of the rheumatic diseases High 29555825
2018 Fyn participates in EPO receptor signaling; fyn-null mice exhibit reduced Tyr-phosphorylation of EPO-R and decreased STAT5 activity. Fyn is also a physiologic repressor of Nrf2; absence of Fyn results in persistent Nrf2 activation and over-activation of Jak2-Akt-mTOR pathway in erythroblasts. fyn-knockout mice, EPO-R phosphorylation assay, STAT5 activity assay, Nrf2 activation analysis, Jak2-Akt-mTOR pathway western blot, rapamycin rescue American journal of hematology Medium 30252956
2018 SHROOM3 interacts with FYN via a critical SH3-binding domain (distinct from its ROCK-binding domain) in human podocytes; this interaction activates Fyn kinase and is required for downstream nephrin phosphorylation. Shroom3 knockdown in mice induces albuminuria with foot process effacement. Co-immunoprecipitation with endogenous proteins, SH3-binding domain mutagenesis, Shroom3 knockdown mice, nephrin phosphorylation assay, electron microscopy Journal of the American Society of Nephrology High 30341149
2019 Frontotemporal dementia P301L mutant Tau immobilizes Fyn in dendritic spines, altering its motion state distribution and nanoclustering; wild-type Tau removal increases Fyn mobility in dendritic shafts, demonstrating that Tau controls the nanoscale organization of Fyn in neuronal dendrites. Single-molecule tracking in mouse hippocampal neurons, Tau knockout/overexpression, P301L Tau expression eLife High 31237563
2019 Fyn kinase activity is localized at plasma membrane microdomains, with its submembrane localization providing a suppressive microenvironment; Fyn localized outside its microdomains shows hyperactivity, and relatively high Fyn activity is observed in perinuclear regions. FRET biosensor in live cells, membrane microdomain targeting with different lipid anchor motifs, growth factor stimulation ACS sensors Medium 30588803
2022 Fyn and TOM1L1 are recruited to a subset of clathrin-coated pits (CCPs) with unique lifetimes; Fyn and TOM1L1 perturbation impairs EGF-stimulated Akt2 (but not Akt1) phosphorylation and mediates TOM1L1/Fyn-dependent SHIP2 recruitment to CCPs, linking clathrin-coated pits to specific Akt isoform signaling. Live-cell imaging, siRNA knockdown, phospho-Akt isoform analysis, SHIP2 recruitment assay The Journal of cell biology Medium 35238864
2023 FYN directly binds to TOPK/PBK in gastric cancer cells and phosphorylates TOPK at Tyr-272; TOPK-Y272F mutation impairs FYN-TOPK interaction and abolishes TOPK phosphorylation. Phosphorylated TOPK in turn controls HSPB1 and p-HSPB1(Ser15) levels, promoting gastric cancer proliferation and metastasis. Co-IP, pull-down, immunofluorescence co-localization, 32P-labeled isotope kinase assay, in vitro kinase assay, phospho-specific antibody generation, TOPK knockout mice, phosphoproteomics Journal of experimental & clinical cancer research High 37016377

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2012 Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease. Nature 3725 23128233
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
1994 Different signal transduction properties of KDR and Flt1, two receptors for vascular endothelial growth factor. The Journal of biological chemistry 1298 7929439
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2003 Genome-wide survey of human alternative pre-mRNA splicing with exon junction microarrays. Science (New York, N.Y.) 1117 14684825
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
1992 Impaired long-term potentiation, spatial learning, and hippocampal development in fyn mutant mice. Science (New York, N.Y.) 927 1361685
2020 A reference map of the human binary protein interactome. Nature 849 32296183
1993 Human Sos1: a guanine nucleotide exchange factor for Ras that binds to GRB2. Science (New York, N.Y.) 772 8493579
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2017 Roles of tau protein in health and disease. Acta neuropathologica 716 28386764
2006 Force sensing by mechanical extension of the Src family kinase substrate p130Cas. Cell 711 17129785
2012 Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition. Cell 708 22939624
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
1990 Association between the PDGF receptor and members of the src family of tyrosine kinases. Cell 649 1696179
2006 A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Cell 610 16713569
1998 A requirement for caveolin-1 and associated kinase Fyn in integrin signaling and anchorage-dependent cell growth. Cell 590 9741627
1995 Proline-rich (PxxP) motifs in HIV-1 Nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef+ viruses but not for down-regulation of CD4. The EMBO journal 525 7859737
2014 Recurrent mutations in epigenetic regulators, RHOA and FYN kinase in peripheral T cell lymphomas. Nature genetics 515 24413734
2013 Metabotropic glutamate receptor 5 is a coreceptor for Alzheimer aβ oligomer bound to cellular prion protein. Neuron 480 24012003
2007 C-type lectin DC-SIGN modulates Toll-like receptor signaling via Raf-1 kinase-dependent acetylation of transcription factor NF-kappaB. Immunity 472 17462920
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2006 Virus-induced Abl and Fyn kinase signals permit coxsackievirus entry through epithelial tight junctions. Cell 429 16413486
2010 Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (New York, N.Y.) 421 20360068
2005 Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome research 409 16344560
2001 Characterization of Fyn-mediated tyrosine phosphorylation sites on GluR epsilon 2 (NR2B) subunit of the N-methyl-D-aspartate receptor. The Journal of biological chemistry 404 11024032
1991 Membrane glycoprotein IV (CD36) is physically associated with the Fyn, Lyn, and Yes protein-tyrosine kinases in human platelets. Proceedings of the National Academy of Sciences of the United States of America 403 1715582
1996 Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain. Cell 395 8681387
1995 Specific and redundant roles of Src and Fyn in organizing the cytoskeleton. Nature 302 7617039
2015 Fyn inhibition rescues established memory and synapse loss in Alzheimer mice. Annals of neurology 252 25707991
1986 yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family. Proceedings of the National Academy of Sciences of the United States of America 243 3526330
2002 Process outgrowth of oligodendrocytes is promoted by interaction of fyn kinase with the cytoskeletal protein tau. The Journal of neuroscience : the official journal of the Society for Neuroscience 209 11826099
2003 Fyn binds to and phosphorylates the kidney slit diaphragm component Nephrin. The Journal of biological chemistry 190 12668668
1999 Compartmentation of Fyn kinase with glycosylphosphatidylinositol-anchored molecules in oligodendrocytes facilitates kinase activation during myelination. The Journal of biological chemistry 185 10506155
2005 Absence of Fyn and Src causes a reeler-like phenotype. The Journal of neuroscience : the official journal of the Society for Neuroscience 164 16162939
2008 Activation of oligodendroglial Fyn kinase enhances translation of mRNAs transported in hnRNP A2-dependent RNA granules. The Journal of cell biology 161 18490510
2011 Regulation of NMDA receptors by the tyrosine kinase Fyn. The FEBS journal 160 21985328
2001 A unique role for Fyn in CNS myelination. The Journal of neuroscience : the official journal of the Society for Neuroscience 153 11245687
2006 Regulation of dendritic branching and spine maturation by semaphorin3A-Fyn signaling. The Journal of neuroscience : the official journal of the Society for Neuroscience 142 16540575
2002 Striatal enriched phosphatase 61 dephosphorylates Fyn at phosphotyrosine 420. The Journal of biological chemistry 139 11983687
2017 Targeting Fyn Kinase in Alzheimer's Disease. Biological psychiatry 132 28709498
2003 Scaffolding of Fyn kinase to the NMDA receptor determines brain region sensitivity to ethanol. The Journal of neuroscience : the official journal of the Society for Neuroscience 121 12736333
1998 Fyn, a Src family tyrosine kinase. The international journal of biochemistry & cell biology 116 9839441
2009 An integrin-contactin complex regulates CNS myelination by differential Fyn phosphorylation. The Journal of neuroscience : the official journal of the Society for Neuroscience 110 19625508
1992 p21rasGAP association with Fyn, Lyn, and Yes in thrombin-activated platelets. The Journal of biological chemistry 106 1544885
2009 Endocytosis of flotillin-1 and flotillin-2 is regulated by Fyn kinase. Journal of cell science 101 19258392
2015 Fyn is an important molecule in cancer pathogenesis and drug resistance. Pharmacological research 100 26305432
1999 YopH dephosphorylates Cas and Fyn-binding protein in macrophages. Microbial pathogenesis 99 10502464
2017 Lyn and Fyn function as molecular switches that control immunoreceptors to direct homeostasis or inflammation. Nature communications 95 28811476
2006 Fibronectin rigidity response through Fyn and p130Cas recruitment to the leading edge. Molecular biology of the cell 91 16597701
2012 Local phosphocycling mediated by LOK/SLK restricts ezrin function to the apical aspect of epithelial cells. The Journal of cell biology 86 23209304
2015 Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction. The Journal of cell biology 80 26459603
2014 SLK-dependent activation of ERMs controls LGN-NuMA localization and spindle orientation. The Journal of cell biology 78 24958772
1999 Induction of apoptosis by SLK, a Ste20-related kinase. Oncogene 76 10602516
1983 Acetone, Isopropanol, and Butanol Production by Clostridium beijerinckii (syn. Clostridium butylicum) and Clostridium aurantibutyricum. Applied and environmental microbiology 75 16346237
2016 Tyrosine kinase FYN negatively regulates NOX4 in cardiac remodeling. The Journal of clinical investigation 74 27525436
2020 Fyn Tyrosine Kinase as Harmonizing Factor in Neuronal Functions and Dysfunctions. International journal of molecular sciences 69 32580508
2007 Chemically diverse toxicants converge on Fyn and c-Cbl to disrupt precursor cell function. PLoS biology 69 17298174
2011 Fyn requires HnRNPA2B1 and Sam68 to synergistically regulate apoptosis in pancreatic cancer. Carcinogenesis 68 21642356
2011 Fyn kinase in brain diseases and cancer: the search for inhibitors. Current medicinal chemistry 66 21651487
2021 GSK-3β, FYN, and DYRK1A: Master Regulators in Neurodegenerative Pathways. International journal of molecular sciences 65 34445804
2018 Tyrosine kinase Fyn promotes osteoarthritis by activating the β-catenin pathway. Annals of the rheumatic diseases 65 29555825
2014 Myceliophthora thermophila syn. Sporotrichum thermophile: a thermophilic mould of biotechnological potential. Critical reviews in biotechnology 61 25025273
2003 Cooperative roles of Fyn and cortactin in cell migration of metastatic murine melanoma. The Journal of biological chemistry 60 13129922
2009 Laminin alters fyn regulatory mechanisms and promotes oligodendrocyte development. The Journal of neuroscience : the official journal of the Society for Neuroscience 57 19776266
2006 Structure of human Fyn kinase domain complexed with staurosporine. Biochemical and biophysical research communications 55 16782058
2023 FYN: emerging biological roles and potential therapeutic targets in cancer. Journal of translational medicine 54 36740671
1998 CD73 expression and fyn-dependent signaling on murine lymphocytes. European journal of immunology 51 9808167
1977 beta-Lactamase and beta-lactam antibiotics resistance in acinetobacter anitratum (syn: A. calcoaceticus). The Journal of antibiotics 50 201599
2005 The Ste20-like kinase SLK is required for cell cycle progression through G2. The Journal of biological chemistry 48 16236704
1985 Isolation and Characterization of an Elicitor of Necrosis Isolated from Intercellular Fluids of Compatible Interactions of Cladosporium fulvum (Syn. Fulvia fulva) and Tomato. Plant physiology 47 16664113
2016 Understanding the cellular roles of Fyn-related kinase (FRK): implications in cancer biology. Cancer metastasis reviews 46 27067725
2006 Src, Fyn and Yes play differential roles in VEGF-mediated endothelial cell events. Angiogenesis 46 16400523
2014 Species boundaries and nomenclature of Rhizopus arrhizus (syn. R. oryzae). Mycoses 45 25266947
2009 Srcasm inhibits Fyn-induced cutaneous carcinogenesis with modulation of Notch1 and p53. Cancer research 45 19934324
2008 Comparative phosphoproteomics of zebrafish Fyn/Yes morpholino knockdown embryos. Molecular & cellular proteomics : MCP 45 18550893
2019 Frontotemporal dementia mutant Tau promotes aberrant Fyn nanoclustering in hippocampal dendritic spines. eLife 43 31237563
2008 BCR-ABL1 mediates up-regulation of Fyn in chronic myelogenous leukemia. Blood 43 18180382
2015 Fyn is an intermediate kinase that BDNF utilizes to promote oligodendrocyte myelination. Glia 42 26449489
2006 On the origin of syn- and metachronous urothelial carcinomas. European urology 42 17123702
2019 NT5DC2 promotes tumorigenicity of glioma stem-like cells by upregulating fyn. Cancer letters 40 30978441
2017 Suppression of protein tyrosine phosphatase N23 predisposes to breast tumorigenesis via activation of FYN kinase. Genes & development 39 29066500
2012 Ste20-like kinase SLK, at the crossroads: a matter of life and death. Cell adhesion & migration 39 23154402
2003 Deletion of the fyn-kinase gene alters behavioral sensitivity to ethanol. Alcoholism, clinical and experimental research 39 12878908
2015 Ecological differentiation, lack of hybrids involving diploids, and asymmetric gene flow between polyploids in narrow contact zones of Senecio carniolicus (syn. Jacobaea carniolica, Asteraceae). Ecology and evolution 38 25859328
2020 Aucklandia costus (Syn. Saussurea costus): Ethnopharmacology of an endangered medicinal plant of the himalayan region. Journal of ethnopharmacology 37 32730877
2012 SLK-mediated phosphorylation of paxillin is required for focal adhesion turnover and cell migration. Oncogene 37 23128389
2009 The Ste20-like kinase SLK is required for ErbB2-driven breast cancer cell motility. Oncogene 36 19525980
2008 Functions of Fyn kinase in the completion of meiosis in mouse oocytes. Developmental biology 36 19118543
2018 Fyn kinase is a novel modulator of erythropoietin signaling and stress erythropoiesis. American journal of hematology 35 30252956
2015 Fyn-phosphorylated PIKE-A binds and inhibits AMPK signaling, blocking its tumor suppressive activity. Cell death and differentiation 35 26001218
2003 p250GAP, a neural RhoGAP protein, is associated with and phosphorylated by Fyn. Biochemical and biophysical research communications 35 12788081
1996 Involvement of Fyn tyrosine kinase in progression of cytokinesis of B lymphocyte progenitor. The Journal of cell biology 35 8567733
2010 The involvement of Fyn kinase in resumption of the first meiotic division in mouse oocytes. Cell cycle (Georgetown, Tex.) 33 20372074
2004 Lipid rafts: resolution of the "fyn problem"? Molecular immunology 33 15220001
1990 Structure and expression of fyn genes in Xenopus laevis. Oncogene 33 2179818
2000 A critical role for p59(fyn) in CD2-based signal transduction. European journal of immunology 32 11093170
2016 MOBP levels are regulated by Fyn kinase and affect the morphological differentiation of oligodendrocytes. Journal of cell science 31 26801084
2020 Perhexiline Demonstrates FYN-mediated Antitumor Activity in Glioblastoma. Molecular cancer therapeutics 30 32430486
2010 Fyn kinase is involved in cleavage furrow ingression during meiosis and mitosis. Reproduction (Cambridge, England) 30 20841362
2016 KLF5 promotes cell migration by up-regulating FYN in bladder cancer cells. FEBS letters 29 26786295
2006 Srcasm corrects Fyn-induced epidermal hyperplasia by kinase down-regulation. The Journal of biological chemistry 29 17046829
2016 Ligand-induced growth and compaction of CD36 nanoclusters enriched in Fyn induces Fyn signaling. Journal of cell science 28 27694211
2007 Unc119 regulates myofibroblast differentiation through the activation of Fyn and the p38 MAPK pathway. Journal of immunology (Baltimore, Md. : 1950) 28 17579091
2021 Src and Fyn regulation of NMDA receptors in health and disease. Neuropharmacology 27 34051267
2017 Myrcia splendens (Sw.) DC. (syn. M. fallax (Rich.) DC.) (Myrtaceae) Essential Oil from Amazonian Ecuador: A Chemical Characterization and Bioactivity Profile. Molecules (Basel, Switzerland) 27 28704964
2015 Fyn in Neurodevelopment and Ischemic Brain Injury. Developmental neuroscience 27 25720756
2012 Fyn promotes Th17 differentiation by regulating the kinetics of RORγt and Foxp3 expression. Journal of immunology (Baltimore, Md. : 1950) 27 22539787
2010 Role of Fyn kinase in oocyte developmental potential. Reproduction, fertility, and development 26 20591331
2008 The Gammaherpesvirus m2 protein manipulates the Fyn/Vav pathway through a multidocking mechanism of assembly. PloS one 26 18301737
2012 Role of FYN kinase in spermatogenesis: defects characteristic of Fyn-null sperm in mice. Biology of reproduction 25 21918125
2008 Ste20-related protein kinase LOSK (SLK) controls microtubule radial array in interphase. Molecular biology of the cell 25 18287541
2015 A NOX2/Egr-1/Fyn pathway delineates new targets for TKI-resistant malignancies. Oncotarget 24 26136341
2020 FYN is required for ARHGEF16 to promote proliferation and migration in colon cancer cells. Cell death & disease 23 32811808
1999 Distinctive roles of Fyn and Lyn in IgD- and IgM-mediated signaling. International immunology 23 10464165
2022 Electrophilic Sulfur Reagent Design Enables Directed syn-Carbosulfenylation of Unactivated Alkenes. Journal of the American Chemical Society 22 35436110
2019 Sensitive FRET Biosensor Reveals Fyn Kinase Regulation by Submembrane Localization. ACS sensors 22 30588803
2018 SHROOM3-FYN Interaction Regulates Nephrin Phosphorylation and Affects Albuminuria in Allografts. Journal of the American Society of Nephrology : JASN 22 30341149
2014 Post-translational regulation of COX2 activity by FYN in prostate cancer cells. Oncotarget 22 24970799
2010 Expression of Fyn kinase modulates EMT in oral cancer cells. Anticancer research 22 20682987
2006 v-Src-dependent down-regulation of the Ste20-like kinase SLK by casein kinase II. The Journal of biological chemistry 22 16837460
2022 Fyn and TOM1L1 are recruited to clathrin-coated pits and regulate Akt signaling. The Journal of cell biology 21 35238864
2011 Regulation of the Ste20-like kinase, SLK: involvement of activation segment phosphorylation. The Journal of biological chemistry 21 22203681
2023 FYN/TOPK/HSPB1 axis facilitates the proliferation and metastasis of gastric cancer. Journal of experimental & clinical cancer research : CR 20 37016377
2019 N6-methyladenosine RNA methylation is involved in virulence of the rice blast fungus Pyricularia oryzae (syn. Magnaporthe oryzae). FEMS microbiology letters 20 30535195
2011 Activity of the Ste20-like kinase, SLK, is enhanced by homodimerization. American journal of physiology. Renal physiology 20 21677149
2009 The Ste20-like kinase SLK promotes p53 transactivation and apoptosis. American journal of physiology. Renal physiology 20 19640899