Affinage

Showing DLG3 — SAP102 is a alias.

DLG3

Disks large homolog 3 · UniProt Q92796

Length
817 aa
Mass
90.3 kDa
Annotated
2026-06-09
58 papers in source corpus 28 papers cited in narrative 28 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

DLG3/SAP102 is a postsynaptic MAGUK scaffold that links NMDA receptors to the submembranous cytomatrix at excitatory synapses, coordinating receptor trafficking and dendritic spine remodeling during synaptogenesis (PMID:8780649, PMID:8702950, PMID:19104036). It binds the C-terminal tail of the NR2/GluN2 subunit through its PDZ domains and recognizes GluN2B through a second, PDZ-independent N-terminal site, and this dual mode of engagement governs both synaptic targeting and synaptic clearance of GluN2B-containing receptors (PMID:8702950, PMID:21209193, PMID:23103165). SAP102 mediates synaptic delivery of AMPA and NMDA receptors selectively during early development, after which PSD-95 assumes these functions, and it can sustain AMPAR transmission through a cornichon-2–dependent mechanism (PMID:19104036, PMID:30067114). Beyond receptor anchoring, SAP102 nucleates a signaling platform: it recruits Pyk2 and the A2A adenosine receptor through its SH3/GK module, mPins to promote NMDAR surface delivery, EphB2/Kalirin-7 to drive PAK-dependent actin reorganization and synapse formation, and the exocyst subunit Sec8 preferentially via PDZ2 (PMID:12576483, PMID:16299499, PMID:23486974, PMID:24509856, PMID:37849738). Its synaptic enrichment and mobility are tuned by CK2 phosphorylation at Ser632 and by JNK3 phosphorylation, and by C-terminal alternative splicing that generates spine-enriched isoforms (PMID:21209193, PMID:25555912, PMID:29282697, PMID:38582451). In polarized epithelia DLG3 recruits the Nedd4/Nedd4-2 E3 ligases via PPxY motifs to drive its own monoubiquitination, apical recruitment and tight-junction formation, while in cancer it engages MST2/LATS1 Hippo signaling and is regulated by COP1-mediated ubiquitination (PMID:21920314, PMID:32593652, PMID:37356109). Loss-of-function variants in DLG3, including a 5'UTR duplication that attenuates translation, cause intellectual disability (PMID:27222290).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1996 High

    Established that SAP102 is a bona fide NMDA receptor-associated scaffold, defining the founding molecular interaction that placed it at the postsynaptic cytomatrix.

    Evidence Reciprocal co-immunoprecipitation from rat brain synaptosomes and cortical synaptic membranes plus in vitro PDZ-domain binding to the NR2B C-terminal tail

    PMID:8702950 PMID:8780649

    Open questions at the time
    • Did not establish functional consequence of the interaction in vivo
    • Did not resolve which PDZ domain dominates binding in cells
  2. 1997 Medium

    Showed SAP102 has functions beyond receptor anchoring by linking it to the APC tumor suppressor and demonstrating cross-species functional conservation with Drosophila Dlg.

    Evidence Yeast two-hybrid and in vitro binding to APC; heterologous rescue of dlg-1 mutant flies

    PMID:9152008 PMID:9188857

    Open questions at the time
    • APC interaction not validated in mammalian neurons
    • Mechanism of tumor/bouton rescue not resolved at the molecular level
  3. 1999 Medium

    Identified Ca2+/calmodulin and p51-nedasin as regulators that can modulate SAP102 interactions, introducing the idea that NMDAR clustering by SAP102 is dynamically controlled.

    Evidence Surface plasmon resonance, pull-down, yeast two-hybrid, and in vitro competition assays

    PMID:10026200 PMID:10542258

    Open questions at the time
    • Regulatory effects shown largely in vitro
    • Physiological calmodulin-dependent switching not demonstrated at synapses
  4. 2000 Medium

    Demonstrated a PDZ-dependent growth-suppressive and adhesion-regulating role for SAP102 in non-neuronal cells, extending its function to cell polarity and proliferation control.

    Evidence Overexpression and domain-deletion analysis in cancer cell lines with beta-catenin readout

    PMID:10797259

    Open questions at the time
    • Overexpression system may not reflect endogenous role
    • APC-independent pathway not molecularly defined
  5. 2005 High

    Defined mPins as a trafficking partner that drives SAP102 and NMDAR delivery to the membrane, linking the scaffold to G-protein regulatory machinery.

    Evidence Co-IP, siRNA knockdown, dominant-negative expression and surface assays in neurons

    PMID:16299499

    Open questions at the time
    • Step in the secretory/trafficking pathway not pinpointed
    • Relationship to PDZ-mediated NMDAR binding not resolved
  6. 2008 High

    Resolved the developmental logic of MAGUK function by showing SAP102 mediates AMPA/NMDA receptor trafficking during synaptogenesis before PSD-95 takes over.

    Evidence In utero electroporation with dual whole-cell electrophysiology at distinct developmental stages in knockout mice

    PMID:19104036

    Open questions at the time
    • Molecular basis of the developmental hand-off to PSD-95 unknown
    • Does not define which interactions drive AMPAR versus NMDAR trafficking
  7. 2011 High

    Uncovered a PDZ-independent N-terminal GluN2B binding site under alternative-splicing control that shapes spine morphology, and showed PDZ binding is required for receptor targeting but not SAP102 clustering itself.

    Evidence Co-IP domain mapping, shRNA knockdown, spine morphology and pharmacological NMDAR blockade; PDZ-mutant expression in neurons

    PMID:21209193 PMID:23178474

    Open questions at the time
    • How the N-terminal site and PDZ binding are coordinated is unclear
    • Splicing regulation upstream signals not identified
  8. 2011 High

    Established a non-neuronal mechanism whereby DLG3 recruits Nedd4/Nedd4-2 via PPxY motifs to undergo monoubiquitination required for apical membrane recruitment and tight-junction formation.

    Evidence AP-MS, co-IP, knockout mouse, immunofluorescence and ubiquitination assays in polarized epithelia

    PMID:21920314

    Open questions at the time
    • Whether ubiquitin-dependent targeting operates in neurons is unknown
    • Deubiquitinase counterpart not identified
  9. 2012 High

    Showed the secondary non-PDZ interaction mediates synaptic clearance of GluN2B receptors and identified the GluN2B residues responsible, defining a dual binding/clearance mechanism.

    Evidence GluN2B mutagenesis, SAP102 knockdown, surface biotinylation and electrophysiology; plus neurobeachin domain-mapping interaction

    PMID:22745750 PMID:23103165

    Open questions at the time
    • Trafficking machinery executing clearance not defined
    • Functional role of neurobeachin binding not established
  10. 2013 High

    Connected SAP102 to EphB/PAK signaling and actin remodeling, showing it is required for ephrinB-driven synapse formation in developing cortex.

    Evidence shRNA knockdown, co-IP of EphB2/Kalirin-7, surface expression, EphB activation and actin analyses

    PMID:23486974

    Open questions at the time
    • Direct versus indirect EphB2 binding not distinguished
    • Link between actin remodeling and receptor trafficking not integrated
  11. 2016 High

    Defined in vivo developmental roles via knockout and linked DLG3 to human intellectual disability through a translation-attenuating 5'UTR variant.

    Evidence Dlg3 knockout mouse electrophysiology and axon quantification; dual luciferase reporter and patient-cell Western blot

    PMID:27222290 PMID:27466188

    Open questions at the time
    • Cellular mechanism linking reduced SAP102 to cognitive deficit unresolved
    • KO phenotypes are subtle, indicating redundancy with other MAGUKs
  12. 2018 Medium

    Identified cornichon-2 as the auxiliary subunit required for SAP102 to rescue AMPAR transmission, refining the MAGUK-AMPAR functional link.

    Evidence Molecular replacement of PSD-95, whole-cell electrophysiology and cornichon-2 knockdown

    PMID:30067114

    Open questions at the time
    • Direct SAP102–CNIH-2 binding not demonstrated
    • Physiological relevance outside replacement paradigm unclear
  13. 2024 Medium

    Consolidated post-translational and structural control of SAP102 synaptic dynamics through CK2/JNK3 phosphorylation, splice-variant enrichment, Sec8/PDZ2 binding, and distinct subsynaptic nanocluster organization.

    Evidence In vitro kinase/FRAP/live-imaging assays; isoform knockdown; Sec8 crystal structure with binding assays; DNA-PAINT super-resolution

    PMID:25555912 PMID:29282697 PMID:37849738 PMID:38582451 PMID:38777601

    Open questions at the time
    • How phosphorylation, splicing and nanocluster positioning integrate is unknown
    • Functional consequence of SAP102-specific nanodomains not established
  14. 2023 Medium

    Extended DLG3 into cancer signaling, implicating it in Hippo-pathway control and COP1-mediated ubiquitination affecting tumor cell proliferation.

    Evidence RIP, co-IP, luciferase reporter, ubiquitination assays and in vivo tumor/proliferation models in breast cancer and glioma cells

    PMID:32593652 PMID:37356109

    Open questions at the time
    • Whether MST2/Hippo and COP1 roles operate in neurons is unknown
    • Direct DLG3–MST2 binding interface not mapped

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the multiple regulatory layers (phosphorylation, ubiquitination, alternative splicing, nanocluster positioning) are integrated to control SAP102 function in vivo, and how this maps onto cognitive phenotypes, remains unresolved.
  • No unified model linking PTMs and splicing to physiological output
  • Mechanism connecting SAP102 loss to intellectual disability undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4
Localization
GO:0005886 plasma membrane 2 GO:0005829 cytosol 1
Pathway
R-HSA-112316 Neuronal System 3 R-HSA-1266738 Developmental Biology 3 R-HSA-162582 Signal Transduction 2
Partners
DLG4EPHB2EXOC4GPSM2GRIN2BKALRNNEDD4PTK2B
Complex memberships
NMDA receptor–MAGUK complexexocyst (via Sec8)

Evidence

Reading pass · 28 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1996 SAP102 interacts in vivo with NMDA receptor complexes: antibodies against NMDA receptors co-immunoprecipitate SAP102 from rat brain synaptosomes, and all three PDZ domains of SAP102 bind the cytoplasmic tail of NR2B in vitro, linking NMDA receptors to the postsynaptic cytomatrix. Co-immunoprecipitation from rat brain synaptosomes; in vitro binding with recombinant proteins containing NR2B carboxy-terminal tail Neuron High 8702950 8780649
1996 SAP102 specifically interacts with the NMDA receptor complex (but not AMPA receptor subunit GluR1) via the last 20 amino acids of the NR2 subunit C-terminal tail, as demonstrated in cortical synaptic plasma membranes. Co-immunoprecipitation from cortical synaptic plasma membranes; hexahistidine fusion protein overlay; synthetic peptide interaction assay The Journal of biological chemistry High 8702950
1997 NE-dlg/SAP102 interacts with the carboxyl-terminal region of the APC tumor suppressor protein, as revealed by yeast two-hybrid screening and in vitro binding assays, and is expressed in non-proliferating neuronal and endocrine cells. Yeast two-hybrid screening; in vitro binding assay Oncogene Medium 9188857
1997 SAP102 functionally substitutes for Drosophila DlgA: when expressed in dlg-1 mutant flies, SAP102 suppresses tumor formation and restores synaptic bouton morphology at larval neuromuscular junctions, requiring neuronal expression. Heterologous expression in Drosophila dlg-1 mutants; morphological analysis of synaptic boutons Mechanisms of development Medium 9152008
1999 NE-dlg/SAP102 interacts with calmodulin in a Ca2+-dependent manner (Kd ~44 nM) via a basic alpha-helix region near the SH3 domain, and also interacts with the GUK-like domain of PSD-95/SAP90 in the presence of Ca2+/calmodulin; Ca2+/calmodulin binding does not modulate the PDZ–NR2B interaction. Surface plasmon resonance; pull-down assay; yeast two-hybrid screening The Journal of biological chemistry High 10026200
1999 A novel SAP102-binding protein, p51-nedasin (predominantly the S splice form), interacts with SAP102 in cell bodies and interferes with the association between SAP102 and NMDA receptor NR2B in vitro, suggesting a regulatory role in NMDA receptor clustering at synapses. Yeast two-hybrid; co-immunoprecipitation; in vitro competition assay The Journal of biological chemistry Medium 10542258
2000 Overexpression of NE-dlg/SAP102 in cancer cell lines induces growth suppression, impairment of cell adhesion, and downregulation of beta-catenin through an APC-independent pathway; the PDZ domains of NE-dlg are required for these effects. Overexpression in cancer cell lines; domain deletion analysis; Western blotting for beta-catenin International journal of cancer Medium 10797259
2001 By electron microscopic immunocytochemistry, SAP102 is localized at postsynaptic densities of spines but also diffusely along presynaptic membranes and in axonal cytoplasm of rat visual cortex, suggesting roles in both receptor anchoring at synapses and receptor shuttling between nonsynaptic and synaptic sites. Electron microscopic immunocytochemistry (quantitative EM-ICC) in rat visual cortex Synapse Medium 11309840
2003 The proline-rich regions of Pyk2 bind the SH3 domain of SAP102 (and PSD-95), recruiting Pyk2 to the NMDA receptor complex; the intramolecular SH3–GK domain interaction in SAP102 restricts Pyk2 binding to the SH3 domain, revealed when the GK domain is removed. Co-immunoprecipitation; pull-down; immunofluorescence co-localization; domain deletion analysis The Journal of biological chemistry Medium 12576483
2005 mPins (mammalian partner of inscuteable) interacts with SAP102 and functions in forming the NMDAR-MAGUK complex; mPins enhances trafficking of SAP102 and NMDARs to the plasma membrane, and dominant-negative mPins or siRNA knockdown of mPins decreases SAP102 in dendrites and reduces NMDAR surface expression. Co-immunoprecipitation; siRNA knockdown; dominant-negative expression; surface expression assays in neurons Nature cell biology High 16299499
2008 SAP102 mediates synaptic trafficking of both AMPA and NMDA receptors during synaptogenesis; after synaptogenesis, PSD-95 assumes these functions. This temporal division was established by in utero electroporation with dual whole-cell electrophysiology at two developmental stages. In utero electroporation; dual whole-cell patch-clamp electrophysiology at distinct developmental stages; knockout mice Proceedings of the National Academy of Sciences of the United States of America High 19104036
2011 SAP102 contains a novel N-terminal NMDA receptor binding site specific for NR2B that is PDZ-domain independent and is regulated by alternative splicing; the N-terminal insert splice variant promotes dendritic spine lengthening and synapse formation at long spines, and blocking NMDA receptor activity prevents this spine lengthening. Co-immunoprecipitation; domain mapping; shRNA knockdown; morphological analysis of dendritic spines; pharmacological NMDA receptor blockade The Journal of neuroscience High 21209193
2011 Dlg3 contributes to apical-basal polarity and tight junction formation in polarized epithelial cells; Dlg3 recruits E3 ubiquitin ligases Nedd4 and Nedd4-2 through its PPxY motifs, leading to Dlg3 monoubiquitination, apical membrane recruitment, and tight junction consolidation. Affinity purification/mass spectrometry of Dlg3 complexes; co-immunoprecipitation; mouse knockout; immunofluorescence; ubiquitination assays Developmental cell High 21920314
2011 SAP102 PDZ domain ligand-binding is not essential for its own synaptic clustering; however, direct PDZ-mediated binding of NMDARs to SAP102 is required for efficient synaptic targeting of NMDA receptor subunits GluN2A and GluN2B. Expression of SAP102 PDZ-binding-deficient mutants in hippocampal neurons; co-expression with GluN2A/GluN2B; synaptic clustering analysis Neuroscience letters Medium 23178474
2012 SAP102 mediates synaptic clearance of GluN2B-containing NMDARs through a secondary non-PDZ interaction; two critical residues on GluN2B responsible for non-PDZ binding to SAP102 were identified, and mutation of these residues or SAP102 knockdown rescues defective surface/synaptic expression of PDZ-binding-deficient GluN2B. Mutagenesis of GluN2B residues; SAP102 siRNA knockdown; surface biotinylation; electrophysiology Cell reports High 23103165
2012 SAP102 binds to the C-terminal part of neurobeachin (Nbea) containing the DUF, PH, BEACH, and WD40 domains; a mutation in Nbea's PH domain that disrupts its PH-BEACH interaction abolishes SAP102 binding. Mass spectrometry identification from mouse brain; co-immunoprecipitation in heterologous cells; domain mapping with mutations PloS one Medium 22745750
2013 SAP102 regulates cortical synapse development through EphB and PAK signaling: SAP102 co-immunoprecipitates EphB2 and Kalirin-7 in neonatal cortex; SAP102 knockdown reduces EphB surface expression, prevents actin reorganization and synapse formation in response to ephrinB, and downregulates PAK activity. Lentivirus shRNA knockdown; co-immunoprecipitation; surface expression analysis; EphB activation assays; actin cytoskeleton analysis The Journal of neuroscience High 23486974
2014 SAP102 interacts with the A2A adenosine receptor C-terminus (via the SH3 and GK domains of SAP102); SAP102 overexpression prevents the A2A receptor from accessing a compartment with restricted (slow) mobility, dependent on a DVELL motif in the A2A receptor C-terminus. Single particle tracking (quantum dot); co-immunoprecipitation; hidden Markov model analysis of diffusion; dominant-negative fragment expression The Journal of biological chemistry Medium 24509856
2015 Synaptic localization of SAP102 is regulated by C-terminal alternative splicing: the I2 splice variant (insert between SH3 and GK domains) is highly enriched at dendritic spines and is developmentally regulated; knockdown of I2-containing SAP102 isoforms differentially affects NMDAR surface expression in a subunit-specific manner. Expression of splice variant constructs; shRNA knockdown of specific isoforms; surface biotinylation; spine morphology analysis The Journal of biological chemistry Medium 25555912
2017 SAP102 synaptic targeting is regulated by phosphorylation at serine 632 by casein kinase II (CK2): Ser632 phosphorylation increases synaptic enrichment of SAP102 and decreases its mobility; elevation of synaptic activity suppresses Ser632 phosphorylation and reduces synaptic enrichment. In vitro kinase assay; phospho-specific analysis in heterologous cells and neurons; FRAP; activity manipulation Molecular neurobiology Medium 29282697
2018 SAP102 regulates synaptic AMPAR function through a CNIH-2 (cornichon-2)-dependent mechanism: SAP102 rescues AMPAR eEPSCs and increases AMPAR mEPSC decay time upon PSD-95 knockdown, and this rescue requires the AMPAR auxiliary subunit cornichon-2. Molecular replacement (shRNA KD of PSD-95 + SAP102 expression); whole-cell patch-clamp electrophysiology; cornichon-2 knockdown Journal of neurophysiology Medium 30067114
2020 MIAT lncRNA promotes methylation of CpG islands in the DLG3 promoter by binding to DNMT1, DNMT3A, and DNMT3B, suppressing DLG3 expression; DLG3 binds MST2 and regulates LATS1, preventing nuclear translocation of YAP to activate the Hippo pathway in breast cancer cells. MS-PCR; RIP assay; dual luciferase reporter; siRNA knockdown; co-immunoprecipitation; in vivo tumor models Cellular signalling Medium 32593652
2023 COP1 E3 ubiquitin ligase binds DLG3 protein and enhances its ubiquitination, promoting glioma cell proliferation, invasion, and migration; DLG3 silencing reverses the inhibitory effect of COP1 knockdown. Co-immunoprecipitation; ubiquitination assay (MG132 treatment); siRNA knockdown; CCK-8, EdU, Transwell assays Neurological research Medium 37356109
2023 Sec8 (exocyst subunit) preferentially binds PDZ2 over PDZ1 and PDZ3 of SAP102 via its C-terminal ITTV motif; a 14-residue 'spacer' bridging ITTV to the Sec8 core is essential, as its deletion abolishes SAP102 binding. 2.5 Å crystal structure of Sec8 C-terminus; in vitro binding assays; domain deletion analysis; molecular modeling Frontiers in cell and developmental biology High 37849738
2024 JNK3 directly phosphorylates SAP102, negatively regulating its synaptic dynamics; JNK activity modulates SAP102 and kainate receptor subunit GluK2 surface expression and trafficking to postsynaptic sites in a neuronal context. In vitro phosphorylation assay; live-cell imaging; pharmacological JNK inhibition; surface expression assays The Journal of biological chemistry Medium 38582451
2024 SAP102 accumulates in high-density subsynaptic nanoclusters that are smaller and denser than PSD-95 nanoclusters; only a subset of SAP102 nanoclusters co-organize with PSD-95, revealing distinct MAGUK nanodomains within individual synapses that differentially associate with the presynaptic release protein Munc13-1. DNA-PAINT super-resolution microscopy in cultured rat neurons The Journal of neuroscience Medium 38777601
2016 Genetic deletion of SAP102 (Dlg3 knockout) in mice reduces the number of thalamocortical axons innervating the somatosensory cortex, causes a transient speeding of NMDA receptor kinetics during the critical period without reducing GluN2B-mediated transmission, and reduces divergence of TC connectivity after the critical period. Dlg3 knockout mouse; in vivo and in vitro electrophysiology; axon quantification Human molecular genetics High 27466188
2016 A 5' UTR duplication variant in DLG3 (dupG, 7 nt upstream of the start codon) attenuates protein translation without affecting mRNA levels, causing intellectual disability; demonstrated by dual luciferase reporter assay and reduced protein in patient blood cells. Dual luciferase reporter assay; Western blot of patient-derived cells; mRNA level analysis European journal of human genetics Medium 27222290

Source papers

Stage 0 corpus · 58 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1996 SAP102, a novel postsynaptic protein that interacts with NMDA receptor complexes in vivo. Neuron 373 8780649
2008 Differential trafficking of AMPA and NMDA receptors by SAP102 and PSD-95 underlies synapse development. Proceedings of the National Academy of Sciences of the United States of America 185 19104036
1996 Interaction of the N-methyl-D-aspartate receptor complex with a novel synapse-associated protein, SAP102. The Journal of biological chemistry 160 8702950
2004 Mutations in the DLG3 gene cause nonsyndromic X-linked mental retardation. American journal of human genetics 135 15185169
2006 Decreased NR1, NR2A, and SAP102 transcript expression in the hippocampus in bipolar disorder. Brain research 125 17113057
2001 Electron microscopic immunocytochemical detection of PSD-95, PSD-93, SAP-102, and SAP-97 at postsynaptic, presynaptic, and nonsynaptic sites of adult and neonatal rat visual cortex. Synapse (New York, N.Y.) 109 11309840
2005 mPins modulates PSD-95 and SAP102 trafficking and influences NMDA receptor surface expression. Nature cell biology 99 16299499
1997 Functional expression of rat synapse-associated proteins SAP97 and SAP102 in Drosophila dlg-1 mutants: effects on tumor suppression and synaptic bouton structure. Mechanisms of development 86 9152008
2010 Reduction in post-synaptic scaffolding PSD-95 and SAP-102 protein levels in the Alzheimer inferior temporal cortex is correlated with disease pathology. Journal of Alzheimer's disease : JAD 83 20634587
1999 Interaction of NE-dlg/SAP102, a neuronal and endocrine tissue-specific membrane-associated guanylate kinase protein, with calmodulin and PSD-95/SAP90. A possible regulatory role in molecular clustering at synaptic sites. The Journal of biological chemistry 79 10026200
2012 SAP102 mediates synaptic clearance of NMDA receptors. Cell reports 69 23103165
2003 Interaction of the tyrosine kinase Pyk2 with the N-methyl-D-aspartate receptor complex via the Src homology 3 domains of PSD-95 and SAP102. The Journal of biological chemistry 62 12576483
1997 Cloning and characterization of NE-dlg: a novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein interacts with the APC protein. Oncogene 53 9188857
2011 NMDA receptor-dependent regulation of dendritic spine morphology by SAP102 splice variants. The Journal of neuroscience : the official journal of the Society for Neuroscience 49 21209193
2013 Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway. The Journal of neuroscience : the official journal of the Society for Neuroscience 48 23486974
2011 Dlg3 trafficking and apical tight junction formation is regulated by nedd4 and nedd4-2 e3 ubiquitin ligases. Developmental cell 46 21920314
2009 A novel mutation in the DLG3 gene encoding the synapse-associated protein 102 (SAP102) causes non-syndromic mental retardation. Neurogenetics 43 19795139
2020 Silence of lncRNA MIAT-mediated inhibition of DLG3 promoter methylation suppresses breast cancer progression via the Hippo signaling pathway. Cellular signalling 35 32593652
2014 Parallel mRNA and microRNA profiling of HEV71-infected human neuroblastoma cells reveal the up-regulation of miR-1246 in association with DLG3 repression. PloS one 32 24739954
1996 Isolation of a gene (DLG3) encoding a second member of the discs-large family on chromosome 17q12-q21. Genomics 31 8824795
2018 Tetrahedral DNA Nanomaterial Regulates the Biological Behaviors of Adipose-Derived Stem Cells via DNA Methylation on Dlg3. ACS applied materials & interfaces 25 30168311
2012 Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin. PloS one 25 22745750
1998 Immunocytochemical localization of the synapse-associated protein SAP102 in the rat retina. The Journal of comparative neurology 25 9674560
2000 NE-dlg, a mammalian homolog of Drosophila dlg tumor suppressor, induces growth suppression and impairment of cell adhesion: possible involvement of down-regulation of beta-catenin by NE-dlg expression. International journal of cancer 23 10797259
1999 A novel NE-dlg/SAP102-associated protein, p51-nedasin, related to the amidohydrolase superfamily, interferes with the association between NE-dlg/SAP102 and N-methyl-D-aspartate receptor. The Journal of biological chemistry 21 10542258
2015 Subunit-specific regulation of N-methyl-D-aspartate (NMDA) receptor trafficking by SAP102 protein splice variants. The Journal of biological chemistry 19 25555912
2024 The prediction of pCR and chemosensitivity for breast cancer patients using DLG3, RADL and Pathomics signatures based on machine learning and deep learning. Translational oncology 17 38805774
2020 LINC01315 Impairs microRNA-211-Dependent DLG3 Downregulation to Inhibit the Development of Oral Squamous Cell Carcinoma. Frontiers in oncology 17 33117688
2014 A two-state model for the diffusion of the A2A adenosine receptor in hippocampal neurons: agonist-induced switch to slow mobility is modified by synapse-associated protein 102 (SAP102). The Journal of biological chemistry 17 24509856
2019 High expression of DLG3 is associated with decreased survival from breast cancer. Clinical and experimental pharmacology & physiology 16 31271664
2011 Fine mapping of Xq11.1-q21.33 and mutation screening of RPS6KA6, ZNF711, ACSL4, DLG3, and IL1RAPL2 for autism spectrum disorders (ASD). Autism research : official journal of the International Society for Autism Research 14 21384559
2024 DLG3 variants caused X-linked epilepsy with/without neurodevelopmental disorders and the genotype-phenotype correlation. Frontiers in molecular neuroscience 13 38249294
2024 Distinct SAP102 and PSD-95 Nano-organization Defines Multiple Types of Synaptic Scaffold Protein Domains at Single Synapses. The Journal of neuroscience : the official journal of the Society for Neuroscience 13 38777601
2017 Skewed X-inactivation in a family with DLG3-associated X-linked intellectual disability. American journal of medical genetics. Part A 13 28777483
2017 Regulation of SAP102 Synaptic Targeting by Phosphorylation. Molecular neurobiology 12 29282697
2016 Altered thalamocortical development in the SAP102 knockout model of intellectual disability. Human molecular genetics 11 27466188
2011 Differential localization of SAP102 and PSD-95 is revealed in hippocampal spines using super-resolution light microscopy. Communicative & integrative biology 11 21509195
1998 DLG3, the gene encoding human neuroendocrine Dlg (NE-Dlg), is located within the 1.8-Mb dystonia-parkinsonism region at Xq13.1. Genomics 11 9598320
2019 A Novel DLG3 Mutation Expanding the Phenotype of X-Linked Intellectual Disability Caused by DLG3 Nonsense Variants. Molecular syndromology 10 32021600
2018 Dynamic SAP102 expression in the hippocampal subregions of rats and APP/PS1 mice of various ages. Journal of anatomy 10 29574717
2018 SAP102 regulates synaptic AMPAR function through a CNIH-2-dependent mechanism. Journal of neurophysiology 10 30067114
2016 A non-coding variant in the 5' UTR of DLG3 attenuates protein translation to cause non-syndromic intellectual disability. European journal of human genetics : EJHG 10 27222290
2012 Ligand binding of PDZ domains has various roles in the synaptic clustering of SAP102 and PSD-95. Neuroscience letters 9 23178474
1999 A recessive mutation leading to vertebral ankylosis in zebrafish is associated with amino acid alterations in the homologue of the human membrane-associated guanylate kinase DLG3. Mechanisms of development 9 10446262
2021 Hypermethylation of DLG3 Promoter Upregulates RAC1 and Activates the PI3K/AKT Signaling Pathway to Promote Breast Cancer Progression. Evidence-based complementary and alternative medicine : eCAM 8 34765009
2020 Triple diagnosis of Wiedemann-Steiner, Waardenburg and DLG3-related intellectual disability association found by WES: A case report. The journal of gene medicine 8 32246869
2024 Identification of a DLG3 stop mutation in the MRX20 family. European journal of human genetics : EJHG 3 38273165
2023 Distinct SAP102 and PSD-95 nano-organization defines multiple types of synaptic scaffold protein domains at single synapses. bioRxiv : the preprint server for biology 3 37745494
2024 Multifaceted roles of DLG3/SAP102 in neurophysiology, neurological disorders and tumorigenesis. Neuroscience 2 39638232
2023 COP1 facilitates the proliferation, invasion, and migration of glioma cells by ubiquitination of DLG3 protein. Neurological research 2 37356109
2011 Polymorphisms in the DLG3 gene is not associated with non-syndromic mental retardation in the Chinese Han population of Qin-Ba mountain. Cellular and molecular neurobiology 2 21369957
2008 Cloning and characterization of E-dlg, a novel splice variant of mouse homologue of the Drosophila discs large tumor suppressor binds preferentially to SAP102. IUBMB life 2 18618587
2025 A New Family with X-Linked Intellectual Disability 90: A Case Report of a Novel DLG3 Variant and Literature Review. Molecular syndromology 1 40881055
2025 Further phenotypical delineation of DLG3-related neurodevelopmental disorders. European journal of human genetics : EJHG 1 40983642
2024 JNK activity modulates postsynaptic scaffold protein SAP102 and kainate receptor dynamics in dendritic spines. The Journal of biological chemistry 1 38582451
2023 Sec8 specifically interacts with the PDZ2 domain of synapse associated protein 102 (SAP102). Frontiers in cell and developmental biology 1 37849738
2019 SAP102 contributes to hyperalgesia formation in the cancer induced bone pain rat model by anchoring NMDA receptors. Neuroscience letters 1 31682872
2024 Retraction: LINC01315 impairs microRNA-211-dependent DLG3 downregulation to inhibit the development of oral squamous cell carcinoma. Frontiers in oncology 0 39026977

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