Affinage

RPSA

Small ribosomal subunit protein uS2 · UniProt P08865

Round 2 corrected
Length
295 aa
Mass
32.9 kDa
Annotated
2026-04-28
130 papers in source corpus 17 papers cited in narrative 17 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RPSA is a dual-function protein that serves as a structural component of the 40S small ribosomal subunit and as a cell-surface laminin receptor with roles in cell adhesion, viral entry, and signal transduction. Cryo-EM structures of the human 80S ribosome position RPSA within the small subunit, while its cell-surface form mediates laminin binding, acts as an entry receptor for adeno-associated virus serotypes 2/3/8/9, and engages amyloid-β42 to promote cytotoxicity (PMID:25901680, PMID:16973587, PMID:24048171). Beyond translation and adhesion, RPSA localizes to the nucleus where it associates with histones H2A, H2B, and H4 and with chromatin DNA, cross-links to polyadenylated mRNA as an RNA-binding protein, and activates HIF-1α through FAK–PI3K and ERK signaling in cancer cells (PMID:9878528, PMID:22681889, PMID:17476462). Heterozygous loss-of-function mutations in RPSA cause isolated congenital asplenia by haploinsufficiency, establishing a non-redundant requirement for this gene in human spleen organogenesis (PMID:23579497, PMID:30072435).

Mechanistic history

Synthesis pass · year-by-year structured walk · 10 steps
  1. 1986 High

    Identification of the laminin receptor mRNA and protein established RPSA as a cell-surface laminin-binding molecule whose expression correlates with receptor surface density, answering the question of whether laminin receptor biosynthesis is transcriptionally regulated.

    Evidence Affinity purification on laminin and lambda gt11 cDNA library screening from human endothelial cells, Northern blot across carcinoma lines

    PMID:2429301

    Open questions at the time
    • Mechanism of conversion from the 37-kDa precursor to the 67-kDa cell-surface form was unknown
    • No structural information on the laminin-binding domain
    • Ribosomal role of the protein not yet recognized
  2. 1996 High

    Gene structure analysis and subcellular localization studies revealed RPSA to be a ribosomal-protein-class gene whose product localizes not only to the cytoplasm and cell surface but also to the nucleus and perichromosomal region, challenging a purely receptor-centric model.

    Evidence Genomic cloning with RNase protection/primer extension (chicken), immunofluorescence and immunoelectron microscopy in mammalian cells

    PMID:8954992 PMID:8985115

    Open questions at the time
    • Nuclear binding partners and functional significance of chromatin association were unresolved
    • Mechanism by which a single polypeptide partitions among ribosome, membrane, and nucleus was unknown
  3. 1998 Medium

    Biochemical assays demonstrated that nuclear RPSA directly binds DNA and interacts with histones H2A, H2B, and H4, providing the first molecular partners for its chromatin-associated function, while loss-of-function experiments showed RPSA is required for cell survival.

    Evidence DNA-cellulose chromatography and GST-histone pull-down assays; antisense/ribozyme knockdown in HeLa cells with apoptosis readout

    PMID:10200442 PMID:9878528

    Open questions at the time
    • Functional consequence of histone binding (e.g., chromatin remodeling, gene regulation) was not determined
    • Whether the survival requirement reflects the ribosomal versus the receptor role was unclear
    • No in vivo validation of nuclear interactions
  4. 2006 High

    Identification of RPSA as a cell-surface receptor for AAV serotypes 2, 3, 8, and 9 expanded its receptor repertoire beyond laminin to include viral entry, validated both in vitro and in vivo in mouse liver.

    Evidence In vitro capsid protein binding, siRNA knockdown of RPSA, in vivo mouse liver transduction with AAV8

    PMID:16973587

    Open questions at the time
    • Co-receptor requirements and post-binding endocytic mechanism were not defined
    • Relative contribution of RPSA versus other AAV receptors (e.g., HSPG) was not quantified in vivo
  5. 2007 Medium

    RPSA was placed upstream of HIF-1α induction via FAK–PI3K and ERK signaling in gastric cancer cells, establishing a signaling axis through which a laminin receptor activates hypoxia-responsive transcription under normoxic conditions.

    Evidence Overexpression and siRNA knockdown of RPSA with HIF-1 reporter assays and pharmacological pathway inhibitors in gastric cancer cells

    PMID:17476462 PMID:19998339

    Open questions at the time
    • Direct physical link between RPSA and FAK was not shown
    • Whether this signaling operates in non-cancer contexts was untested
    • Upstream ligand triggering RPSA signaling in vivo was not identified
  6. 2012 Medium

    UV-crosslinking proteomics identified RPSA as part of the mRNA-bound proteome, establishing it as an RNA-binding protein with potential extra-ribosomal roles in mRNA metabolism.

    Evidence Photoreactive nucleotide-enhanced UV crosslinking with oligo(dT) purification and quantitative mass spectrometry in human cells

    PMID:22681889

    Open questions at the time
    • Specific mRNA targets and functional consequences of mRNA binding outside the ribosome were not determined
    • Whether RNA binding reflects a ribosome-associated or free-protein activity was unclear
  7. 2013 High

    Three landmark advances converged: cryo-EM structures placed RPSA within the 40S subunit of the human 80S ribosome; human genetic studies showed heterozygous RPSA loss-of-function mutations cause isolated congenital asplenia via haploinsufficiency; and RPSA was identified as a cell-surface mediator of Aβ42 cytotoxicity.

    Evidence Cryo-EM of human 80S ribosome; whole-exome sequencing of 18 ICA patients across 8 kindreds; pull-down and shRNA rescue of Aβ42 toxicity

    PMID:23579497 PMID:23636399 PMID:24048171

    Open questions at the time
    • Whether asplenia reflects ribosomal or extra-ribosomal RPSA function was unresolved
    • Structural basis of laminin or Aβ42 binding at the cell surface was not determined
    • Developmental window and cell type requiring RPSA for splenic organogenesis were not identified
  8. 2015 High

    Near-atomic resolution cryo-EM refined RPSA's structural role within the 40S subunit, while functional studies confirmed that RPSA knockdown induces apoptosis across multiple cancer types, reinforcing its pro-survival role.

    Evidence 3.6 Å cryo-EM of human 80S ribosome; siRNA knockdown with two independent sequences in breast and oesophageal cancer lines

    PMID:25901680 PMID:26427016

    Open questions at the time
    • Whether the apoptotic effect of RPSA depletion is mediated through translational impairment, loss of laminin signaling, or both was not dissected
    • No animal model recapitulating cancer-cell RPSA dependency
  9. 2018 High

    Expanded genetic analysis of ICA kindreds identified additional RPSA mutations including 5'-UTR splicing variants and de novo events, confirming autosomal dominant inheritance with incomplete penetrance and broadening the mutational spectrum.

    Evidence Whole-exome and Sanger sequencing with mRNA splicing analysis across 41% of ICA kindreds

    PMID:30072435

    Open questions at the time
    • Modifier genes or environmental factors explaining incomplete penetrance were not identified
    • No animal model of RPSA heterozygous knockout recapitulating asplenia
  10. 2020 Medium

    RPSA was shown to form a functional signaling complex with the TRPM7 ion channel at the plasma membrane, mediating elastin-derived peptide-stimulated cancer cell migration, revealing a new receptor-channel partnership.

    Evidence Co-localization by immunofluorescence, patch-clamp electrophysiology, siRNA knockdown of TRPM7, Boyden chamber migration assay in pancreatic cancer cells

    PMID:32733880

    Open questions at the time
    • Direct physical interaction between RPSA and TRPM7 was inferred from co-localization but not demonstrated by co-immunoprecipitation
    • Stoichiometry and structural basis of the complex were not characterized
    • Relevance of the TRPM7–RPSA axis beyond pancreatic cancer was not tested

Open questions

Synthesis pass · forward-looking unresolved questions
  • The mechanism by which RPSA haploinsufficiency specifically impairs spleen organogenesis — whether through ribosomal dysfunction (ribosomal haploinsufficiency), loss of laminin receptor signaling, or a combined effect — remains an open and central question.
  • No conditional knockout animal model distinguishing ribosomal from extra-ribosomal functions in splenic development
  • Structural basis of RPSA's laminin and Aβ42 binding at the cell surface is unknown
  • Mechanism of 37-kDa to 67-kDa conversion remains unresolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060089 molecular transducer activity 4 GO:0005198 structural molecule activity 3 GO:0003723 RNA binding 2 GO:0001618 virus receptor activity 1 GO:0003677 DNA binding 1
Localization
GO:0005886 plasma membrane 4 GO:0005840 ribosome 3 GO:0005634 nucleus 2 GO:0005694 chromosome 2 GO:0005829 cytosol 1
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-392499 Metabolism of proteins 3 R-HSA-1266738 Developmental Biology 2 R-HSA-1643685 Disease 2 R-HSA-5357801 Programmed Cell Death 2 R-HSA-168256 Immune System 1
Partners
H2AFXH2BHIST1H4ATRPM7
Complex memberships
40S ribosomal small subunit80S ribosome

Evidence

Reading pass · 17 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1986 The laminin receptor (later identified as RPSA/37LRP) was purified and its mRNA (~1700 bases) cloned from human endothelial cells; levels of laminin receptor mRNA correlated with surface receptor number across carcinoma cell lines, suggesting mRNA abundance is a rate-limiting step in biosynthesis of the cell-surface laminin-binding protein. Affinity purification on laminin, lambda gt11 cDNA library screening with anti-receptor monoclonal antibody, cDNA sequencing, Northern blot Proceedings of the National Academy of Sciences of the United States of America High 2429301
1996 The active 37LRP/p40 (RPSA) gene was identified in chicken (the only vertebrate with a single copy); it comprises 7 exons and 6 introns, lacks a TATA box, has multiple transcription start sites, and bears features of ribosomal protein genes. Domain analysis indicated the C-terminal domain is a conserved structure involved in generating the 67-kDa laminin receptor form, while the central core mediates ribosome association. Genomic cloning, RNase protection assay, primer extension, sequence analysis, protein evolutionary comparison DNA and cell biology High 8985115
1996 The laminin-binding protein precursor p40 (LBP-p40/RPSA) localizes not only in the cytoplasm but also in the nucleus, where it is tightly associated with nuclear envelope and chromatin DNA in interphase nuclei and with the perichromosomal region during mitosis. Monoclonal antibody immunofluorescence, subcellular fractionation, immunoelectron microscopy Biochemical and biophysical research communications Medium 8954992
1998 Nuclear LBP-p40 (RPSA) binds double-stranded DNA and associates with histone proteins H2A, H2B, and H4 (but not H3), as demonstrated by DNA-cellulose binding and GST-histone pull-down assays; interaction with these histones confers tighter chromatin binding. DNA-cellulose affinity chromatography, LBP-p40 affinity column, GST-histone fusion pull-down assays Biochemical and biophysical research communications Medium 9878528
1998 Loss of LBP-p40 (RPSA) expression by antisense or ribozyme-mediated knockdown in HeLa cells causes apoptotic cell death under serum-depleted conditions, demonstrating that RPSA is required for cell survival. Antisense cDNA expression, ribozyme delivery via fusigenic viral liposome, Western/Northern blot, colony formation assay, DNA fragmentation assay Cell death and differentiation Medium 10200442
2006 The 37/67-kDa laminin receptor (RPSA/LamR) serves as a cell-surface receptor for adeno-associated virus serotypes 8, 2, 3, and 9; LamR binds AAV8 capsid proteins in vitro and contributes to AAV8 transduction of cultured cells and mouse liver in vivo, with binding mapped to two protein subdomains on the AAV capsid exterior. In vitro capsid protein binding assays, siRNA knockdown, in vivo mouse liver transduction, sequence/deletion mapping Journal of virology High 16973587
2007 MGr1-Ag/37LRP (RPSA) promotes HIF-1α expression and transcriptional activity under non-hypoxic conditions in gastric cancer cells treated with vincristine; forced expression of RPSA upregulates HIF-1α, while siRNA knockdown reduces it; FAK-PI3K and ERK1/2 (p42/44 MAPK) signaling are the major pathways downstream of RPSA leading to HIF-1α induction. Overexpression, siRNA knockdown, Western blot, HIF-1 reporter assays, pharmacological inhibitors (PI3K, MAPK) Molecular and cellular biochemistry Medium 17476462
2010 Hypoxia-induced RPSA (MGr1-Ag/37LRP) expression is driven by ERK/MAPK activation via reactive oxygen species (H2O2); MEK activates HIF-1, which in turn drives RPSA promoter activity; RPSA then mediates enhanced gastric cancer cell adhesion to laminin and resistance to vincristine-induced apoptosis; MEK inhibitor U0126 and RPSA siRNA reverse these effects. MEK overexpression, dominant-negative kinases, MEK inhibitor U0126, ROS scavengers, siRNA, adhesion assays, apoptosis assays International journal of cancer Medium 19998339
2012 RPSA is identified as a component of the mRNA-bound proteome in human cells; it cross-links to mRNA upon UV irradiation and co-purifies with polyadenylated transcripts, establishing RPSA as an RNA-binding protein beyond its ribosomal role. Photoreactive nucleotide-enhanced UV crosslinking and oligo(dT) purification, quantitative mass spectrometry Molecular cell Medium 22681889
2013 Heterozygous loss-of-function mutations in RPSA cause isolated congenital asplenia (ICA) in humans by haploinsufficiency; seven different mutations (nonsense, frameshift, missense) were identified in 18 patients from 8 kindreds, establishing an essential and non-redundant role for RPSA in human spleen organogenesis. Whole-exome sequencing, Sanger sequencing, expression studies in patient cells, segregation analysis Science (New York, N.Y.) High 23579497
2013 RPSA (LRP/LR) co-localizes and physically interacts with Aβ42 at the cell surface; antibody blockade of RPSA or shRNA-mediated knockdown significantly rescues cell viability and proliferation in Aβ42-treated cells, indicating RPSA mediates Aβ42 cytotoxicity. Immunofluorescence co-localization, pull-down assays, MTT viability assay, BrdU proliferation assay, Annexin-V apoptosis assay, shRNA knockdown Scientific reports Medium 24048171
2013 Structures of human and Drosophila 80S ribosomes determined by cryo-EM reveal RPSA as a component of the 40S small ribosomal subunit, and illuminate metazoan-specific ribosomal features including an inner protein layer and flexible RNA outer layer. High-resolution cryo-electron microscopy, atomic model building Nature High 23636399
2015 Near-atomic structure of the human 80S ribosome at 3.6 Å resolution (reaching 2.9 Å in stable regions) by cryo-EM reveals RPSA's position in the 40S subunit, details of subunit interfaces, tRNA binding sites, and ribosome rotational dynamics. Single-particle cryo-electron microscopy at near-atomic resolution, atomic model building Nature High 25901680
2015 siRNA-mediated knockdown of RPSA (LRP/LR) significantly reduces viability of breast (MCF-7, MDA-MB 231) and oesophageal (WHCO1) cancer cells by inducing apoptosis, as shown by phosphatidylserine externalization (Annexin-V) and nuclear morphological changes; results confirmed with two independent siRNA sequences targeting different RPSA mRNA regions. siRNA knockdown (siRNA-LAMR1 and esiRNA-RPSA), MTT assay, Annexin-V/FITC assay, Hoechst staining, Western blot PloS one Medium 26427016
2015 RPSA protein was identified as binding aflatoxin B1 (AFB1) by immobilized affinity chromatography from mouse liver total protein; the RPSA–AFB1 interaction was validated by ELISA in vitro and laser confocal immunofluorescence in vivo, suggesting RPSA is an AFB1-binding protein in hepatocytes. Immobilized affinity chromatography (BSA-AFB1 on PVDF), LC-MS/MS identification, ELISA, laser confocal immunofluorescence Journal of hazardous materials Medium 26372695
2018 Additional RPSA mutations causing isolated congenital asplenia include mutations in the 5'-UTR that disrupt mRNA splicing, as well as 11 new protein-coding mutations; overall 41% of ICA kindreds carry RPSA mutations; some mutations show incomplete penetrance, and 7 de novo mutations were identified, confirming autosomal dominant inheritance. Whole-exome and Sanger sequencing, mRNA splicing analysis, segregation analysis, de novo mutation confirmation Proceedings of the National Academy of Sciences of the United States of America High 30072435
2020 RPSA and TRPM7 (transient receptor potential melastatin-related 7 channel) co-localize at the plasma membrane of human pancreatic cancer cells and form a functional complex; elastin-derived peptides (EDPs) stimulate TRPM7 currents and promote cell migration via this TRPM7/RPSA complex, as TRPM7 siRNA abrogates EDP-induced migration. Boyden chamber migration assay, siRNA knockdown of TRPM7, patch-clamp electrophysiology, co-localization by immunofluorescence Frontiers in cell and developmental biology Medium 32733880

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Towards a proteome-scale map of the human protein-protein interaction network. Nature 2090 16189514
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2012 The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. Molecular cell 973 22681889
2005 Nucleolar proteome dynamics. Nature 934 15635413
2004 Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nature biotechnology 916 15592455
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2002 Directed proteomic analysis of the human nucleolus. Current biology : CB 780 11790298
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2017 Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Science (New York, N.Y.) 533 28302793
2003 LRP: role in vascular wall integrity and protection from atherosclerosis. Science (New York, N.Y.) 483 12690199
2013 Structures of the human and Drosophila 80S ribosome. Nature 481 23636399
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2010 Global analysis of TDP-43 interacting proteins reveals strong association with RNA splicing and translation machinery. Journal of proteome research 422 20020773
1998 SA, JA, ethylene, and disease resistance in plants. Current opinion in plant biology 410 10066607
2005 Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome research 409 16344560
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
1998 Inducible inactivation of hepatic LRP gene by cre-mediated recombination confirms role of LRP in clearance of chylomicron remnants. The Journal of clinical investigation 398 9449704
2004 The molecular mechanics of eukaryotic translation. Annual review of biochemistry 396 15189156
2015 Structure of the human 80S ribosome. Nature 380 25901680
2006 The 37/67-kilodalton laminin receptor is a receptor for adeno-associated virus serotypes 8, 2, 3, and 9. Journal of virology 340 16973587
1988 The human laminin receptor is a member of the integrin family of cell adhesion receptors. Science (New York, N.Y.) 323 2970671
2010 Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics. Cell 318 21145461
2018 Syringic acid (SA) ‒ A Review of Its Occurrence, Biosynthesis, Pharmacological and Industrial Importance. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 313 30243088
1986 Altered levels of laminin receptor mRNA in various human carcinoma cells that have different abilities to bind laminin. Proceedings of the National Academy of Sciences of the United States of America 283 2429301
2000 Integrins as receptors for laminins. Microscopy research and technique 278 11054877
1993 The interleukin-12 subunit p40 specifically inhibits effects of the interleukin-12 heterodimer. European journal of immunology 275 8103745
2000 Role of tissue plasminogen activator receptor LRP in hippocampal long-term potentiation. The Journal of neuroscience : the official journal of the Society for Neuroscience 235 10632583
2008 SRF and myocardin regulate LRP-mediated amyloid-beta clearance in brain vascular cells. Nature cell biology 226 19098903
2005 The low density lipoprotein receptor-related protein (LRP) is a novel beta-secretase (BACE1) substrate. The Journal of biological chemistry 210 15749709
1999 Cytomegalovirus US2 destroys two components of the MHC class II pathway, preventing recognition by CD4+ T cells. Nature medicine 207 10470081
2007 The LDL receptor-related protein (LRP) family: an old family of proteins with new physiological functions. Annals of medicine 197 17457719
2002 Phytochrome signalling modulates the SA-perceptive pathway in Arabidopsis. The Plant journal : for cell and molecular biology 187 12100485
1997 In vivo production and function of IL-12 p40 homodimers. Journal of immunology (Baltimore, Md. : 1950) 184 9127002
2005 Beyond endocytosis: LRP function in cell migration, proliferation and vascular permeability. Journal of thrombosis and haemostasis : JTH 160 16102056
2008 Genome-scale reconstruction of the Lrp regulatory network in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 149 19052235
2013 Ribosomal protein SA haploinsufficiency in humans with isolated congenital asplenia. Science (New York, N.Y.) 143 23579497
2010 Macrophage LRP-1 controls plaque cellularity by regulating efferocytosis and Akt activation. Arteriosclerosis, thrombosis, and vascular biology 135 20150557
1997 Human cytomegalovirus US2 destabilizes major histocompatibility complex class I heavy chains. Journal of virology 133 9060656
1991 Characterization of Lrp, and Escherichia coli regulatory protein that mediates a global response to leucine. The Journal of biological chemistry 129 2040596
2005 Sorting nexin 17 facilitates LRP recycling in the early endosome. The EMBO journal 128 16052210
2006 LRP in amyloid-beta production and metabolism. Annals of the New York Academy of Sciences 123 17185504
1995 A consensus sequence for binding of Lrp to DNA. Journal of bacteriology 118 7665463
2001 Crystal structure of the Lrp-like transcriptional regulator from the archaeon Pyrococcus furiosus. The EMBO journal 116 11230123
1997 Soluble low density lipoprotein receptor-related protein (LRP) circulates in human plasma. The Journal of biological chemistry 115 9295345
2014 An AXL/LRP-1/RANBP9 complex mediates DC efferocytosis and antigen cross-presentation in vivo. The Journal of clinical investigation 101 24509082
1996 Effects of nutrition and growth rate on Lrp levels in Escherichia coli. Journal of bacteriology 94 8955316
2002 IL-12: the role of p40 versus p75. Scandinavian journal of immunology 86 12100467
2010 Functional analysis of the p40 and p75 proteins from Lactobacillus casei BL23. Journal of molecular microbiology and biotechnology 85 21178363
2008 Evidence for altered LRP/RAGE expression in Alzheimer lesion pathogenesis. Current Alzheimer research 84 18855584
2010 LRP-1 promotes cancer cell invasion by supporting ERK and inhibiting JNK signaling pathways. PloS one 80 20644732
1999 Characterization of the soluble form of the low density lipoprotein receptor-related protein (LRP). Experimental cell research 80 10471328
2013 Role of pncA and rpsA gene sequencing in detection of pyrazinamide resistance in Mycobacterium tuberculosis isolates from southern China. Journal of clinical microbiology 79 24131688
2002 Runaway cell death, but not basal disease resistance, in lsd1 is SA- and NIM1/NPR1-dependent. The Plant journal : for cell and molecular biology 77 11844114
2015 Plasma membrane profiling defines an expanded class of cell surface proteins selectively targeted for degradation by HCMV US2 in cooperation with UL141. PLoS pathogens 74 25875600
1997 Do cMOAT (MRP2), other MRP homologues, and LRP play a role in MDR? Seminars in cancer biology 72 9441949
2006 The role of megalin (LRP-2/Gp330) during development. Developmental biology 71 16828734
1994 Regulation of the Escherichia coli lrp gene. Journal of bacteriology 67 8144448
2008 LRP-1 silencing prevents malignant cell invasion despite increased pericellular proteolytic activities. Molecular and cellular biology 63 18316405
2000 Involvement of low-density lipoprotein receptor-related protein (LRP) in the clearance of factor VIII in von Willebrand factor-deficient mice. Blood 63 10688827
2019 Structural and free energy landscape of novel mutations in ribosomal protein S1 (rpsA) associated with pyrazinamide resistance. Scientific reports 60 31097767
2003 Expression of MRP1, LRP and Pgp in breast carcinoma patients treated with preoperative chemotherapy. Breast cancer research and treatment 59 14572157
2009 OsBWMK1 mediates SA-dependent defense responses by activating the transcription factor OsWRKY33. Biochemical and biophysical research communications 57 19607808
2016 sRNA roles in regulating transcriptional regulators: Lrp and SoxS regulation by sRNAs. Nucleic acids research 56 27137887
2006 Alkylation of duplex DNA in nucleosome core particles by duocarmycin SA and yatakemycin. Nature chemical biology 55 16415862
2008 Clathrin and LRP-1-independent constitutive endocytosis and recycling of uPAR. PloS one 54 19008962
2008 Leucine-responsive regulatory protein (Lrp) acts as a virulence repressor in Salmonella enterica serovar Typhimurium. Journal of bacteriology 54 19074398
1989 Structure of the low-density lipoprotein receptor-related protein (LRP) promoter. Biochimica et biophysica acta 54 2597675
2005 Down-regulation of hepatic LDL receptor-related protein (LRP) in chronic renal failure. Kidney international 53 15698441
2001 Membrane-specific, host-derived factors are required for US2- and US11-mediated degradation of major histocompatibility complex class I molecules. The Journal of biological chemistry 52 11717308
2003 Low density lipoprotein receptor-related protein (LRP) is required for lactoferrin-enhanced collagen gel contractile activity of human fibroblasts. The Journal of biological chemistry 50 12672816
1992 The lrp gene product regulates expression of lysU in Escherichia coli K-12. Journal of bacteriology 48 1569010
2017 Anti-tubercular Activity of Pyrazinamide is Independent of trans-Translation and RpsA. Scientific reports 46 28733601
2016 Engineering of an Lrp family regulator SACE_Lrp improves erythromycin production in Saccharopolyspora erythraea. Metabolic engineering 46 27794466
2002 Binding of urokinase to low density lipoprotein-related receptor (LRP) regulates vascular smooth muscle cell contraction. The Journal of biological chemistry 46 12171938
2010 ERK/MAPK activation involves hypoxia-induced MGr1-Ag/37LRP expression and contributes to apoptosis resistance in gastric cancer. International journal of cancer 45 19998339
2015 IL12B (p40) Gene Polymorphisms Contribute to Ustekinumab Response Prediction in Psoriasis. Dermatology (Basel, Switzerland) 44 26678060
2014 LRP-1: functions, signaling and implications in kidney and other diseases. International journal of molecular sciences 44 25514242
2004 LRP and PDGF signaling: a pathway to atherosclerosis. Trends in cardiovascular medicine 44 15030790
2011 Regulatory and pathogenesis roles of Mycobacterium Lrp/AsnC family transcriptional factors. Journal of cellular biochemistry 43 21608015
2002 The HCMV gene products US2 and US11 target MHC class I molecules for degradation in the cytosol. Current topics in microbiology and immunology 42 12224515
1998 LBP-p40 binds DNA tightly through associations with histones H2A, H2B, and H4. Biochemical and biophysical research communications 42 9878528
2015 Mutations in the pncA and rpsA genes among 77 Mycobacterium tuberculosis isolates in Kazakhstan. The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease 40 25574916
2004 The L1 retroelement-related p40 protein induces p38delta MAP kinase. Autoimmunity 39 15115313
2014 Regulation of LRP-1 expression: make the point. Pathologie-biologie 38 24661974
2016 Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction. Frontiers in plant science 37 27471515
1996 Analysis of nuclear localization of laminin binding protein precursor p40 (LBP/p40). Biochemical and biophysical research communications 37 8954992
2006 The role of LRP and H-NS in transcription regulation: involvement of synergism, allostery and macromolecular crowding. Journal of molecular biology 35 17196617
1997 ERD2 proteins mediate ER retention of the HNEL signal of LRP's receptor-associated protein (RAP). Journal of cell science 35 9010785
2005 Cooperative binding of the leucine-responsive regulatory protein (Lrp) to DNA. Journal of molecular biology 34 15571719
2000 LDL receptor-related protein (LRP) in Alzheimer's disease: towards a unified theory of pathogenesis. Microscopy research and technique 34 10936878
2020 TRPM7/RPSA Complex Regulates Pancreatic Cancer Cell Migration. Frontiers in cell and developmental biology 33 32733880
2017 The Global Regulators Lrp, LeuO, and HexA Control Secondary Metabolism in Entomopathogenic Bacteria. Frontiers in microbiology 33 28261170
2003 Modulation of the LDL receptor and LRP levels by HIV protease inhibitors. Journal of lipid research 31 12837856
2011 Association of ApoE and LRP mRNA levels with dementia and AD neuropathology. Neurobiology of aging 29 21676498
1999 The equine herpesvirus 1 Us2 homolog encodes a nonessential membrane-associated virion component. Journal of virology 29 10074198
1996 Identification of the active gene coding for the metastasis-associated 37LRP/p40 multifunctional protein. DNA and cell biology 29 8985115
2019 Development of DNA vaccines encoding ribosomal proteins (RplL and RpsA) against Nocardia seriolae infection in fish. Fish & shellfish immunology 28 31830563
2015 Knockdown of LRP/LR Induces Apoptosis in Breast and Oesophageal Cancer Cells. PloS one 28 26427016
2007 Protease nexin 1 and its receptor LRP modulate SHH signalling during cerebellar development. Development (Cambridge, England) 28 17409116
2003 The pseudorabies virus Us2 protein, a virion tegument component, is prenylated in infected cells. Journal of virology 28 14581565
1998 The induction of apoptosis in HeLa cells by the loss of LBP-p40. Cell death and differentiation 27 10200442
2019 miR403a and SA Are Involved in NbAGO2 Mediated Antiviral Defenses Against TMV Infection in Nicotiana benthamiana. Genes 26 31336929
2018 Incomplete penetrance for isolated congenital asplenia in humans with mutations in translated and untranslated RPSA exons. Proceedings of the National Academy of Sciences of the United States of America 26 30072435
2015 The Global Transcription Factor Lrp Controls Virulence Modulation in Xenorhabdus nematophila. Journal of bacteriology 26 26170407
2008 The interplay between MAMP and SA signaling. Plant signaling & behavior 25 19513222
2012 Sa-Lrp from Sulfolobus acidocaldarius is a versatile, glutamine-responsive, and architectural transcriptional regulator. MicrobiologyOpen 24 23255531
2008 Polymorphisms of APOE and LRP genes in Brazilian individuals with Alzheimer disease. Alzheimer disease and associated disorders 24 18317248
2007 Involvement of MGr1-Ag/37LRP in the vincristine-induced HIF-1 expression in gastric cancer cells. Molecular and cellular biochemistry 24 17476462
1999 Expression of the lung resistance protein (LRP) in primary breast cancer. Anticancer research 24 10697509
2006 Localization of ERK/MAP kinase is regulated by the alphaherpesvirus tegument protein Us2. Journal of virology 23 16809321
2019 Patented therapeutic approaches targeting LRP/LR for cancer treatment. Expert opinion on therapeutic patents 22 31722579
2018 Pyrazinamide-resistant mycobacterium tuberculosis isolates from Khyber Pakhtunkhwa and rpsA mutations. Journal of biological regulators and homeostatic agents 22 29921403
2017 Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation. Journal of cell science 22 28743740
2013 Anti-LRP/LR specific antibody IgG1-iS18 and knock-down of LRP/LR by shRNAs rescue cells from Aβ42 induced cytotoxicity. Scientific reports 22 24048171
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2020 Gibbs Free Energy Calculation of Mutation in PncA and RpsA Associated With Pyrazinamide Resistance. Frontiers in molecular biosciences 20 32328498
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2000 The preparation and catalytic properties of recombinant human prostate-specific antigen (rPSA). Biochimica et biophysica acta 19 10962094