Affinage

RPS8

Small ribosomal subunit protein eS8 · UniProt P62241

Length
208 aa
Mass
24.2 kDa
Annotated
2026-06-10
8 papers in source corpus 2 papers cited in narrative 2 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 1/1 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RPS8 is a component of the 40S small ribosomal subunit that contributes to translational control and stress responses. It physically associates with CDK11p46, a kinase of the PITSLRE family, without serving as its substrate, and the two proteins synergistically suppress both cap-dependent and IRES-dependent translation while sensitizing cells to Fas ligand-induced apoptosis (PMID:21371428). Beyond this translational/apoptotic axis, no further mechanistic detail of RPS8 in mammalian systems has been characterized in the available corpus.

Mechanistic history

Synthesis pass · year-by-year structured walk · 2 steps
  1. 2011 Medium

    Established that RPS8 is not merely a structural ribosomal protein but a partner that, with CDK11p46, actively represses translation and promotes apoptosis, linking a ribosomal protein to translational shutdown and cell death.

    Evidence His pull-down and mass spectrometry with reciprocal Co-IP (in vitro and in vivo), translation reporter assays, and Fas ligand apoptosis assays in human cells

    PMID:21371428

    Open questions at the time
    • Molecular mechanism by which the RPS8–CDK11p46 pair suppresses translation is not defined
    • Whether the interaction occurs on assembled ribosomes or as free protein is unresolved
    • No structural model of the RPS8–CDK11p46 interface
  2. 2024 Low

    Extended RPS8 function to innate antiviral defense by showing it restricts viral replication and is targeted by a viral non-coding leader RNA, indicating ribosomal proteins can serve as direct antiviral effectors antagonized by viral RNA.

    Evidence RNA-protein binding assay plus overexpression/knockdown and viral replication assays in a fish (Snakehead vesiculovirus) system

    PMID:38432538

    Open questions at the time
    • Single lab, single OE/KD dataset in a fish model with limited mechanistic detail
    • Molecular basis of how leader RNA binding neutralizes RPS8 antiviral activity is unknown
    • Conservation of this antiviral role in mammals is untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • Whether the translational-suppression/apoptotic role and the antiviral role of RPS8 share a common molecular mechanism, and how either relates to its core function in the 40S subunit, remains unresolved.
  • No unifying mechanism linking translation control, apoptosis, and antiviral activity
  • No structural or biochemical characterization of RPS8 within the ribosome in these contexts

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 1 GO:0005198 structural molecule activity 1
Localization
GO:0005840 ribosome 1
Partners
CDK11B
Complex memberships
40S ribosomal subunit

Evidence

Reading pass · 2 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2011 RPS8 (ribosomal protein S8) physically associates with CDK11p46 (a 46 kDa isoform of the PITSLRE kinase family), as identified by His pull-down and mass spectrometry and confirmed in vitro and in vivo. RPS8 is not a substrate of CDK11p46. Together, RPS8 and CDK11p46 synergistically suppress translation in both cap-dependent and IRES-dependent manners, and sensitize cells to Fas ligand-induced apoptosis. His pull-down, mass spectrometry, co-immunoprecipitation (in vitro and in vivo), translation reporter assays, cell apoptosis assay Biochemical and biophysical research communications Medium 21371428
2024 Fish RPS8 (40S ribosomal protein S8) acts as a host antiviral factor against Snakehead vesiculovirus (SHVV): overexpression of RPS8 inhibited SHVV replication, while knockdown of RPS8 promoted it. The SHVV leader RNA (30-nt ncRNA) physically interacts with RPS8 and counteracts its antiviral effect, thereby promoting viral replication. RNA-protein binding assay, overexpression and knockdown experiments, viral replication assays Fish & shellfish immunology Low 38432538

Source papers

Stage 0 corpus · 8 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Soybean phytophthora resistance gene Rps8 maps closely to the Rps3 region. The Journal of heredity 36 15958793
2013 RPS8--a new informative DNA marker for phylogeny of Babesia and Theileria parasites in China. PloS one 20 24244571
2011 CDK11p46 and RPS8 associate with each other and suppress translation in a synergistic manner. Biochemical and biophysical research communications 17 21371428
2022 RXLR effector gene Avr3a from Phytophthora sojae is recognized by Rps8 in soybean. Molecular plant pathology 10 35150190
2015 A PCR-RFLP Assay targeting RPS8 gene for the discrimination between bovine Babesia and Theileria species in China. Parasites & vectors 7 26382041
2013 Discrimination between ovine Babesia and Theileria species in China based on the ribosomal protein S8 (RPS8) gene. Veterinary parasitology 7 23747103
2024 Snakehead vesiculovirus (SHVV) leader RNA interacts with host antiviral factors RPS8 and L13a and promotes virus replication. Fish & shellfish immunology 1 38432538
2026 Microbial degradation of thifensulfuron-methyl by Klebsiella grimontii ES8 and its degradative enzyme kg-RutF: Mechanistic insights and bioremediation potential. Pesticide biochemistry and physiology 0 41629013

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