Affinage

RPS27

Small ribosomal subunit protein eS27 · UniProt P42677

Round 2 corrected
Length
84 aa
Mass
9.5 kDa
Annotated
2026-04-28
130 papers in source corpus 8 papers cited in narrative 9 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RPS27 (eS27) is a zinc-finger-containing structural component of the eukaryotic 40S ribosomal subunit that participates in both canonical translation and extraribosomal regulation of the p53–MDM2 axis. The 83-amino-acid protein was purified from mammalian ribosomes, shown to harbor a zinc-finger motif, and positioned within the metazoan small subunit by cryo-EM (PMID:947902, PMID:8441676, PMID:23636399). Extraribosomally, RPS27 binds the central acidic domain of MDM2, competes with p53 for MDM2 binding, and inhibits MDM2-mediated p53 ubiquitination, thereby stabilizing p53, while itself being a p53-repressible (not p53-inducible) gene that is not a substrate of MDM2 (PMID:21170087). Loss-of-function mutations in RPS27 impair pre-rRNA processing and cause Diamond–Blackfan anaemia through defective ribosome biogenesis and erythropoietic failure (PMID:25424902).

Mechanistic history

Synthesis pass · year-by-year structured walk · 6 steps
  1. 1976 High

    Establishing RPS27 as a bona fide 40S ribosomal subunit protein resolved its identity among the dozens of small-subunit components and provided the first biochemical characterization.

    Evidence Ion-exchange chromatography, gel filtration, SDS-PAGE, and amino acid composition analysis of rat liver 40S ribosomes

    PMID:947902

    Open questions at the time
    • No primary sequence or gene structure available
    • No functional role beyond structural membership inferred
  2. 1993 High

    Determination of the full 83-amino-acid sequence revealed a zinc-finger-like motif, suggesting RPS27 has a metal-coordinating structural element unusual among ribosomal proteins, and the presence of a 5'TOP element placed its mRNA under growth-dependent translational control.

    Evidence cDNA cloning/sequencing, protein sequencing, and Southern blot of rat RPS27

    PMID:8441676

    Open questions at the time
    • No direct evidence that the zinc finger is functionally required
    • No mutagenesis of the 5'TOP element to confirm translational regulation
  3. 2010 High

    Identifying RPS27 as a direct MDM2 interactor that stabilizes p53 by blocking MDM2-mediated ubiquitination established an extraribosomal tumor-suppressive function and connected ribosomal stress sensing to the p53 pathway.

    Evidence Co-immunoprecipitation with domain mapping, ubiquitination assays, pulse-chase half-life experiments, and siRNA/ectopic expression in human cell lines

    PMID:21170087

    Open questions at the time
    • No in vivo demonstration of the MDM2–RPS27 axis in tumorigenesis
    • Crystal structure of the RPS27–MDM2 interface not resolved
    • Quantitative stoichiometry of free versus ribosome-bound RPS27 unknown
  4. 2013 High

    Cryo-EM placement of RPS27 within the human 80S ribosome provided the first structural context for its position in the small subunit and its contribution to metazoan-specific ribosomal architecture.

    Evidence High-resolution cryo-EM of the human 80S ribosome

    PMID:23636399

    Open questions at the time
    • Atomic-resolution contacts of RPS27 with rRNA and neighboring proteins not fully detailed
    • Role in translational fidelity or decoding not addressed
  5. 2014 High

    Identification of a causative RPS27 frameshift in Diamond–Blackfan anaemia, supported by pre-rRNA processing defects in knockdown cells and erythropoietic failure in zebrafish, established RPS27 haploinsufficiency as a ribosomopathy mechanism.

    Evidence Whole-exome sequencing of DBA patients, siRNA knockdown with Northern blot/qRT-PCR for pre-rRNA intermediates, zebrafish morpholino with erythrocyte staining

    PMID:25424902

    Open questions at the time
    • Precise step(s) in pre-rRNA processing that require RPS27 not delineated
    • Whether p53 pathway activation mediates the erythroid-selective phenotype not tested in this model
  6. 2014 Medium

    Discovery of recurrent somatic 5'UTR hotspot mutations extending the 5'TOP element in ~10% of melanomas suggested that altered translational control of RPS27 abundance contributes to oncogenesis, though direct functional proof was lacking.

    Evidence Collation of independent melanoma exome sequencing datasets with 5'TOP motif analysis

    PMID:24913145

    Open questions at the time
    • No reporter or ribosome profiling assay demonstrating altered translational output from the mutant 5'TOP
    • Downstream consequence on ribosome stoichiometry or p53 regulation not tested
    • Single genomic discovery without functional reconstitution

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: (1) the structural basis of the RPS27–MDM2 interaction at atomic resolution, (2) whether the zinc-finger motif has a specific rRNA-binding or extraribosomal function, (3) the precise step in pre-rRNA processing that depends on RPS27, and (4) the functional impact of melanoma 5'UTR mutations on RPS27 translation and p53 signaling.
  • Atomic-resolution structure of RPS27–MDM2 complex
  • Zinc-finger mutagenesis studies
  • Mechanistic link between 5'UTR mutations and melanoma biology

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0098772 molecular function regulator activity 2
Localization
GO:0005840 ribosome 3 GO:0005829 cytosol 1
Pathway
R-HSA-392499 Metabolism of proteins 3 R-HSA-1643685 Disease 2 R-HSA-5357801 Programmed Cell Death 2
Partners
MDM2TP53
Complex memberships
40S ribosomal subunit80S ribosome

Evidence

Reading pass · 9 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1976 RPS27 (S27) was isolated as a component of the rat liver 40S ribosomal subunit; its molecular weight was estimated by polyacrylamide gel electrophoresis in SDS, and amino acid composition was determined, establishing it as a bona fide small-subunit ribosomal protein. Ion-exchange chromatography (carboxymethylcellulose), gel filtration (Sephadex G-75), SDS-PAGE, amino acid composition analysis The Journal of biological chemistry High 947902
1993 Rat ribosomal protein S27 contains 83 amino acids (MW 9,339 Da) and harbors a zinc finger-like motif, suggesting a structural role involving metal coordination; 4–6 gene copies were detected in the genome and the mRNA is ~620 nucleotides long. cDNA cloning and sequencing, amino acid sequencing of purified protein, Southern hybridization for gene copy number Nucleic acids research High 8441676
1995 Mammalian RPS27 is characterized by a zinc finger domain among its structural motifs; the protein is conserved across eukaryotes and its mRNA contains features typical of ribosomal protein transcripts (5' TOP element). Comparative sequence analysis of 75 rat ribosomal proteins, identification of conserved motifs Biochemistry and cell biology Medium 8722009
2010 Human RPS27 binds to MDM2 via its N-terminal region, interacting with the central acidic domain of MDM2; RPS27 forms an in vivo triplex complex with MDM2 and p53, competing with p53 for MDM2 binding. Ectopic RPS27 expression inhibits MDM2-mediated p53 ubiquitination and increases p53 protein levels by extending its half-life. Co-immunoprecipitation, domain mapping, ubiquitination assays, pulse-chase (half-life measurement), siRNA knockdown, ectopic expression Oncogene High 21170087
2010 Unlike RPS27L, RPS27 is a p53-repressible (not inducible) target gene; RPS27 protein is not a substrate of MDM2 (in contrast to RPS27L) and is not short-lived, indicating divergent regulation despite shared MDM2-binding capacity. Promoter-reporter assays, immunoblot after p53 activation, protein stability assays Oncogene Medium 21170087
2013 Cryo-EM structures of the human 80S ribosome placed RPS27 within the small (40S) ribosomal subunit, revealing its position and contribution to the metazoan-specific structural layers of the ribosome. High-resolution cryo-electron microscopy Nature High 23636399
2014 A frameshift deletion in RPS27 was identified in a Diamond-Blackfan anaemia (DBA) patient; in vitro knockdown of RPS27 disturbed pre-ribosomal RNA processing, and a zebrafish rps27 morpholino model showed impaired erythrocyte production and developmental defects, establishing RPS27 haploinsufficiency as causative for DBA through ribosome biogenesis failure. Whole-exome sequencing, in vitro siRNA knockdown with pre-rRNA processing analysis (Northern/qRT-PCR), zebrafish morpholino knockdown with erythrocyte staining British journal of haematology High 25424902
2014 A recurrent somatic hotspot mutation in the 5' UTR of RPS27 was identified in ~10% of melanoma samples; the mutation expands the 5' terminal oligopyrimidine (5'TOP) element, a motif that regulates expression of ribosomal protein mRNAs in response to growth signals, potentially sensitizing RPS27 transcript abundance to mitogenic regulation. Collation of exome sequencing datasets from independent melanoma cohorts, 5'TOP motif analysis Oncotarget Medium 24913145
2019 Shrimp (Marsupenaeus japonicus) RPS27 (MjRPS27) activates the NF-κB pathway (promoting nuclear translocation of Dorsal and Relish and expression of antimicrobial peptides) in response to white spot syndrome virus (WSSV) infection; MjRPS27 also directly interacts with WSSV envelope proteins VP19, VP24, and VP28, inhibiting virion assembly. Knockdown increased viral replication; overexpression reduced it. RNA interference knockdown, overexpression, co-immunoprecipitation (RPS27 with viral envelope proteins), nuclear translocation assays, survival assay in vivo Frontiers in immunology Medium 31921103

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 2861 17081983
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2012 The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. Molecular cell 973 22681889
2005 Nucleolar proteome dynamics. Nature 934 15635413
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2013 Structures of the human and Drosophila 80S ribosome. Nature 481 23636399
2010 Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine. The Journal of cell biology 453 20624901
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2004 The molecular mechanics of eukaryotic translation. Annual review of biochemistry 396 15189156
2010 Characterization of the proteome, diseases and evolution of the human postsynaptic density. Nature neuroscience 370 21170055
1996 The Saccharomyces cerevisiae spindle pole body duplication gene MPS1 is part of a mitotic checkpoint. The Journal of cell biology 367 8567717
1998 Inactivation of the mitogen-activated protein kinase Mps1 from the rice blast fungus prevents penetration of host cells but allows activation of plant defense responses. Proceedings of the National Academy of Sciences of the United States of America 347 9770551
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2012 Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins. Nature 319 22810586
2010 Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics. Cell 318 21145461
2013 Endogenous purification reveals GREB1 as a key estrogen receptor regulatory factor. Cell reports 307 23403292
2012 Phosphoregulation of Spc105 by Mps1 and PP1 regulates Bub1 localization to kinetochores. Current biology : CB 298 22521787
2012 MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components. Nature cell biology 291 22660415
2001 Mps1 is a kinetochore-associated kinase essential for the vertebrate mitotic checkpoint. Cell 281 11461704
2012 A high-throughput approach for measuring temporal changes in the interactome. Nature methods 273 22863883
2004 Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. Nature biotechnology 266 15146197
1995 Structure and evolution of mammalian ribosomal proteins. Biochemistry and cell biology = Biochimie et biologie cellulaire 265 8722009
2011 A directed protein interaction network for investigating intracellular signal transduction. Science signaling 258 21900206
2008 Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment. Cell 241 18243099
2017 Optimized fragmentation schemes and data analysis strategies for proteome-wide cross-link identification. Nature communications 221 28524877
2014 Ribosomal protein s15 phosphorylation mediates LRRK2 neurodegeneration in Parkinson's disease. Cell 221 24725412
2012 Viral immune modulators perturb the human molecular network by common and unique strategies. Nature 219 22810585
2009 A genome-wide short hairpin RNA screening of jurkat T-cells for human proteins contributing to productive HIV-1 replication. The Journal of biological chemistry 211 19460752
2002 Human Mps1 kinase is required for the spindle assembly checkpoint but not for centrosome duplication. The EMBO journal 208 11927556
2009 Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. Science signaling 207 19738201
2010 Small-molecule kinase inhibitors provide insight into Mps1 cell cycle function. Nature chemical biology 190 20383151
2015 CELL DIVISION CYCLE. Competition between MPS1 and microtubules at kinetochores regulates spindle checkpoint signaling. Science (New York, N.Y.) 180 26068855
2011 Aurora B potentiates Mps1 activation to ensure rapid checkpoint establishment at the onset of mitosis. Nature communications 180 21587233
2012 The MPS1 family of protein kinases. Annual review of biochemistry 178 22482908
2015 CELL DIVISION CYCLE. Kinetochore attachment sensed by competitive Mps1 and microtubule binding to Ndc80C. Science (New York, N.Y.) 164 26068854
2010 Mps1 directs the assembly of Cdc20 inhibitory complexes during interphase and mitosis to control M phase timing and spindle checkpoint signaling. The Journal of cell biology 160 20624902
2014 Mad1 kinetochore recruitment by Mps1-mediated phosphorylation of Bub1 signals the spindle checkpoint. Genes & development 159 24402315
2008 Mps1 kinase activity restrains anaphase during an unperturbed mitosis and targets Mad2 to kinetochores. The Journal of cell biology 156 18541701
2003 Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores. Molecular biology of the cell 140 12686615
2017 A sequential multi-target Mps1 phosphorylation cascade promotes spindle checkpoint signaling. eLife 124 28072388
2010 Ribosomal protein S27-like and S27 interplay with p53-MDM2 axis as a target, a substrate and a regulator. Oncogene 113 21170087
2015 Plk1 and Mps1 Cooperatively Regulate the Spindle Assembly Checkpoint in Human Cells. Cell reports 109 26119734
2014 PP2A-B56 opposes Mps1 phosphorylation of Knl1 and thereby promotes spindle assembly checkpoint silencing. The Journal of cell biology 107 25246613
2017 Functional characterization of CFI-402257, a potent and selective Mps1/TTK kinase inhibitor, for the treatment of cancer. Proceedings of the National Academy of Sciences of the United States of America 100 28270606
2013 Effects of the selective MPS1 inhibitor MPS1-IN-3 on glioblastoma sensitivity to antimitotic drugs. Journal of the National Cancer Institute 100 23940287
2007 Mps1 kinase promotes sister-kinetochore bi-orientation by a tension-dependent mechanism. Current biology : CB 100 18060784
2013 Characterization of novel MPS1 inhibitors with preclinical anticancer activity. Cell death and differentiation 96 23933817
2005 Chemical genetics reveals a role for Mps1 kinase in kinetochore attachment during mitosis. Current biology : CB 93 15668173
2016 Novel Mps1 Kinase Inhibitors with Potent Antitumor Activity. Molecular cancer therapeutics 91 26832791
2018 Leader of the SAC: molecular mechanisms of Mps1/TTK regulation in mitosis. Open biology 86 30111590
1999 Involvement of Arabidopsis thaliana ribosomal protein S27 in mRNA degradation triggered by genotoxic stress. The EMBO journal 84 9889204
2022 MPS1 inhibition primes immunogenicity of KRAS-LKB1 mutant lung cancer. Cancer cell 83 36150391
2014 Loss of function mutations in RPL27 and RPS27 identified by whole-exome sequencing in Diamond-Blackfan anaemia. British journal of haematology 83 25424902
2011 Characterization of the cellular and antitumor effects of MPI-0479605, a small-molecule inhibitor of the mitotic kinase Mps1. Molecular cancer therapeutics 83 21980130
2007 Autophosphorylation-dependent activation of human Mps1 is required for the spindle checkpoint. Proceedings of the National Academy of Sciences of the United States of America 79 18083840
2010 Chemical genetic inhibition of Mps1 in stable human cell lines reveals novel aspects of Mps1 function in mitosis. PloS one 78 20422024
1993 Zinc finger-like motifs in rat ribosomal proteins S27 and S29. Nucleic acids research 75 8441676
2017 Mps1 Regulates Kinetochore-Microtubule Attachment Stability via the Ska Complex to Ensure Error-Free Chromosome Segregation. Developmental cell 72 28441529
1976 The isolation of eukaryotic ribosomal proteins. The purification and characterization of the 40 S ribosomal subunit proteins S2, S3, S4, S5, S6, S7, S8, S9, S13, S23/S24, S27, and S28. The Journal of biological chemistry 72 947902
2007 Mps1 activation loop autophosphorylation enhances kinase activity. The Journal of biological chemistry 70 17728254
2010 A chemical tool box defines mitotic and interphase roles for Mps1 kinase. The Journal of cell biology 69 20624898
2004 Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1. Chromosoma 68 15235793
2017 Aerobic and heterotrophic nitrogen removal by Enterobacter cloacae CF-S27 with efficient utilization of hydroxylamine. Bioresource technology 67 28242385
2016 Mps1 Mediated Phosphorylation of Hsp90 Confers Renal Cell Carcinoma Sensitivity and Selectivity to Hsp90 Inhibitors. Cell reports 67 26804907
2007 Preventing the degradation of mps1 at centrosomes is sufficient to cause centrosome reduplication in human cells. Molecular biology of the cell 66 17804818
2011 Quantitative mass spectrometry analysis reveals similar substrate consensus motif for human Mps1 kinase and Plk1. PloS one 64 21533207
2004 Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1. Genes & development 63 15231734
1977 Isolation of eukaryotic ribosomal proteins. Purification and characterization of the 40 S ribosomal subunit proteins Sa, Sc, S3a, S3b, S5', S9, S10, S11, S12, S14, S15, S15', S16, S17, S18, S19, S20, S21, S26, S27', and S29. The Journal of biological chemistry 60 925037
2007 Activation of Mps1 promotes transforming growth factor-beta-independent Smad signaling. The Journal of biological chemistry 58 17452325
1997 The yeast CDC37 gene interacts with MPS1 and is required for proper execution of spindle pole body duplication. The Journal of cell biology 57 9060463
2015 Dynamic localization of Mps1 kinase to kinetochores is essential for accurate spindle microtubule attachment. Proceedings of the National Academy of Sciences of the United States of America 56 26240331
2013 Structure-based design of orally bioavailable 1H-pyrrolo[3,2-c]pyridine inhibitors of mitotic kinase monopolar spindle 1 (MPS1). Journal of medicinal chemistry 56 24256217
2006 MPS1-dependent mitotic BLM phosphorylation is important for chromosome stability. Proceedings of the National Academy of Sciences of the United States of America 56 16864798
2007 Ribosomal protein S27-like, a p53-inducible modulator of cell fate in response to genotoxic stress. Cancer research 53 18056458
2015 Targeting MPS1 Enhances Radiosensitization of Human Glioblastoma by Modulating DNA Repair Proteins. Molecular cancer research : MCR 51 25722303
2012 CDK-dependent potentiation of MPS1 kinase activity is essential to the mitotic checkpoint. Current biology : CB 51 22245000
1998 New alleles of the yeast MPS1 gene reveal multiple requirements in spindle pole body duplication. Molecular biology of the cell 51 9529376
2019 CDK1-CCNB1 creates a spindle checkpoint-permissive state by enabling MPS1 kinetochore localization. The Journal of cell biology 50 30674582
2008 Crystal structure of the catalytic domain of the mitotic checkpoint kinase Mps1 in complex with SP600125. The Journal of biological chemistry 48 18480048
2005 The meiotic defects of mutants in the Drosophila mps1 gene reveal a critical role of Mps1 in the segregation of achiasmate homologs. Current biology : CB 47 15823541
2017 Protein Phosphatase 1 inactivates Mps1 to ensure efficient Spindle Assembly Checkpoint silencing. eLife 46 28463114
2015 Molecular basis underlying resistance to Mps1/TTK inhibitors. Oncogene 46 26364596
2014 Ribosomal protein S27-like is a physiological regulator of p53 that suppresses genomic instability and tumorigenesis. eLife 46 25144937
2013 VDAC3 and Mps1 negatively regulate ciliogenesis. Cell cycle (Georgetown, Tex.) 45 23388454
2010 N-terminal regions of Mps1 kinase determine functional bifurcation. The Journal of cell biology 45 20368617
2009 Phosphoregulation of human Mps1 kinase. The Biochemical journal 45 18680479
2013 Selective inhibition of pancreatic ductal adenocarcinoma cell growth by the mitotic MPS1 kinase inhibitor NMS-P715. Molecular cancer therapeutics 44 24282275
2016 AAV Gene Therapy for MPS1-associated Corneal Blindness. Scientific reports 42 26899286
2008 Regulation of kinetochore recruitment of two essential mitotic spindle checkpoint proteins by Mps1 phosphorylation. Molecular biology of the cell 42 18923149
2014 A highly recurrent RPS27 5'UTR mutation in melanoma. Oncotarget 41 24913145
2009 MULTIPOLAR SPINDLE 1 (MPS1), a novel coiled-coil protein of Arabidopsis thaliana, is required for meiotic spindle organization. The Plant journal : for cell and molecular biology 40 19500302
2008 A xylanase with high pH stability from Streptomyces sp. S27 and its carbohydrate-binding module with/without linker-region-truncated versions. Applied microbiology and biotechnology 40 19107475
2007 B-Raf(V600E) signaling deregulates the mitotic spindle checkpoint through stabilizing Mps1 levels in melanoma cells. Oncogene 40 18071315
2021 Kinetochore-bound Mps1 regulates kinetochore-microtubule attachments via Ndc80 phosphorylation. The Journal of cell biology 36 34647959
2017 TC Mps1 12, a novel Mps1 inhibitor, suppresses the growth of hepatocellular carcinoma cells via the accumulation of chromosomal instability. British journal of pharmacology 36 28299790
2020 Treating Cancer by Spindle Assembly Checkpoint Abrogation: Discovery of Two Clinical Candidates, BAY 1161909 and BAY 1217389, Targeting MPS1 Kinase. Journal of medicinal chemistry 35 32338514
2018 Mps1 inhibitors synergise with low doses of taxanes in promoting tumour cell death by enhancement of errors in cell division. British journal of cancer 35 29736010
2013 Ribosomal protein S27-like in colorectal cancer: a candidate for predicting prognoses. PloS one 35 23826192
2009 Structural and mechanistic insights into Mps1 kinase activation. Journal of cellular and molecular medicine 34 19120698
2012 Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1. Molecular biology of the cell 33 22553353
2019 Checkpoint signaling and error correction require regulation of the MPS1 T-loop by PP2A-B56. The Journal of cell biology 32 31511308
2011 Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1. Proceedings of the National Academy of Sciences of the United States of America 32 22184248
2016 Whole-genome duplication increases tumor cell sensitivity to MPS1 inhibition. Oncotarget 31 26637805
2015 Mitotic Checkpoint Kinase Mps1 Has a Role in Normal Physiology which Impacts Clinical Utility. PloS one 30 26398286
2010 Biophysical and X-ray crystallographic analysis of Mps1 kinase inhibitor complexes. Biochemistry 30 20099905
2018 Ribosomal protein S27-like regulates autophagy via the β-TrCP-DEPTOR-mTORC1 axis. Cell death & disease 28 30425236
2010 Genetic and biochemical characterization of a protease-resistant mesophilic β-mannanase from Streptomyces sp. S27. Journal of industrial microbiology & biotechnology 28 20686915
2014 Mps1 phosphorylation of condensin II controls chromosome condensation at the onset of mitosis. The Journal of cell biology 27 24934155
2009 Mps1 as a link between centrosomes and genomic instability. Environmental and molecular mutagenesis 27 19274768
2009 Cloning, characterization, and antifungal activity of an endo-1,3-beta-D: -glucanase from Streptomyces sp. S27. Applied microbiology and biotechnology 27 19697016
2008 Cloning, expression, and characterization of a new Streptomyces sp. S27 xylanase for which xylobiose is the main hydrolysis product. Applied biochemistry and biotechnology 27 19002659
2016 Rapid Discovery of Pyrido[3,4-d]pyrimidine Inhibitors of Monopolar Spindle Kinase 1 (MPS1) Using a Structure-Based Hybridization Approach. Journal of medicinal chemistry 26 27055065
2016 Structural basis of reversine selectivity in inhibiting Mps1 more potently than aurora B kinase. Proteins 26 27699881
2017 Novel pyrrolopyrimidines as Mps1/TTK kinase inhibitors for breast cancer. Bioorganic & medicinal chemistry 25 28259529
2015 Naturally Occurring Mutations in the MPS1 Gene Predispose Cells to Kinase Inhibitor Drug Resistance. Cancer research 25 26202014
2013 Chk1 and Mps1 jointly regulate correction of merotelic kinetochore attachments. Journal of cell science 25 23321637
2012 Diaminopyridine-based potent and selective mps1 kinase inhibitors binding to an unusual flipped-Peptide conformation. ACS medicinal chemistry letters 25 24900510
2020 A common molecular mechanism underlies the role of Mps1 in chromosome biorientation and the spindle assembly checkpoint. EMBO reports 24 32307893
2020 Ribosomal Protein S27/Metallopanstimulin-1 (RPS27) in Glioma-A New Disease Biomarker? Cancers 24 32349320
2000 Evolutionarily distinct classes of S27 ribosomal proteins with differential mRNA expression in rat hypothalamus. Journal of neurochemistry 24 10820185
2020 A Biosensor for the Mitotic Kinase MPS1 Reveals Spatiotemporal Activity Dynamics and Regulation. Current biology : CB 23 32888483
2019 RPS27, a sORF-Encoded Polypeptide, Functions Antivirally by Activating the NF-κB Pathway and Interacting With Viral Envelope Proteins in Shrimp. Frontiers in immunology 23 31921103