| 2007 |
Rpf2 and Rrs1 form a ribonucleoprotein neighborhood in preribosomes together with ribosomal proteins rpL5, rpL11, and 5S rRNA; Rpf2 and Rrs1 are required for recruiting rpL5, rpL11, and 5S rRNA into 90S preribosomal particles, and in their absence, processing of 27SB pre-rRNA is blocked, causing abortive 66S pre-rRNPs to be prematurely released from the nucleolus to the nucleoplasm without cytoplasmic export. |
In vitro binding assays, genetic depletion/co-immunoprecipitation in yeast, subcellular fractionation and localization |
Genes & development |
High |
17938242
|
| 2015 |
Crystal structure of the Rpf2-Rrs1 complex (Aspergillus nidulans) at 1.5 Å reveals that the Brix domain of Rpf2 is completed by Rrs1 to form two anticodon-binding-like domains; the heterodimer makes specific contacts with 5S rRNA, RpL5, and the biogenesis factor Rsa4; flexible C-terminal tails of Rrs1 occupy the central protuberance and block rotation of 25S rRNA and the 5S RNP, explaining why removal of Rpf2-Rrs1 is required for the rearrangements that drive 60S maturation. |
X-ray crystallography at 1.5 Å, fitting into cryo-EM density of pre-60S particle, biochemical binding data |
Nucleic acids research |
High |
26117542
|
| 2015 |
Crystal structure of the Aspergillus nidulans Rpf2-Rrs1 core complex shows the N-terminal Brix domain of Rpf2 interlocked with the N-terminal domain of Rrs1 (whose long α-helix joins the C-terminal half of the Brix domain); gel-shift analysis confirmed direct binding of the Rpf2-Rrs1 complex to 5S rRNA, and mutagenesis identified Rpf2 R236 (R238 in A. nidulans) as critical for this binding. |
X-ray crystallography, EMSA gel-shift assay, site-directed mutagenesis |
Nucleic acids research |
High |
25855814
|
| 2008 |
Human BXDC1 (RPF2) localizes specifically to the nucleolus in HeLa cells and, based on FRAP and RNAi knockdown analyses, functions as a dynamic scaffold protein required for ribosome biogenesis. |
Subcellular fractionation, immunofluorescence, FRAP, RNAi knockdown |
Genes to cells : devoted to molecular & cellular mechanisms |
Medium |
19170763
|
| 2017 |
In Trypanosoma brucei, Rpf2 (TbRpf2) was identified as a component of the 5S RNP via tandem affinity purification/mass spectrometry; it mediates conserved binding interactions with 5S rRNA and L5, and additionally interacts with trypanosome-specific proteins P34 and P37; RNAi knockdown of TbRpf2 is lethal and disrupts ribosome formation. |
Tandem affinity purification, mass spectrometry, RNAi knockdown with growth and ribosome assembly phenotype |
mSphere |
Medium |
29062898
|
| 2023 |
In colorectal cancer cells, RPF2 overexpression upregulates ABCB1 (MDR1) expression and promotes chemotherapy resistance; RPF2 regulates MYCN (an upstream regulator of ABCB1), and CARM1 was found to directly bind MYCN in a manner regulated by RPF2, placing RPF2 in a RPF2→CARM1–MYCN→ABCB1 pathway. |
RPF2 overexpression/knockdown in CRC cell lines, Western blot, co-immunoprecipitation of CARM1 and MYCN |
Oncology reports |
Low |
37997821
|
| 2024 |
RPF2 promotes epithelial-mesenchymal transition (EMT) and metastasis in colorectal cancer cells via activation of the AKT/GSK-3β signaling pathway; CARM1 was identified as a key downstream effector of RPF2, and selective CARM1 inhibition suppressed RPF2-induced AKT/GSK-3β activation and EMT; these findings were confirmed in vitro and in vivo. |
Stable RPF2 overexpression/knockdown cell lines, CARM1 inhibitor treatment, in vitro migration/invasion assays, in vivo xenograft experiments, Western blot |
Experimental cell research |
Low |
39674359
|