Affinage

Showing RPA1 — RPA70 is a alias.

RPA1

Replication protein A 70 kDa DNA-binding subunit · UniProt P27694

Length
616 aa
Mass
68.1 kDa
Annotated
2026-06-10
100 papers in source corpus 39 papers cited in narrative 37 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RPA1 (RPA70) is the large subunit of the heterotrimeric single-stranded DNA-binding protein RPA and is an essential, evolutionarily conserved factor required for chromosomal DNA replication, with loss-of-function causing replication failure in yeast and immunodepletion blocking replication in Xenopus extracts (PMID:2192864, PMID:8308077). Its four OB-fold domains separate two genetically distinct activities: high-affinity ssDNA binding through internal DNA-binding domains and a protein-interaction hub in the N-terminal OB-fold domain that is dispensable for replication but required for the DNA damage response (PMID:18469000). Through this architecture RPA1 directly engages the replication and repair machinery — the DNA polymerase alpha-primase p48 subunit, primer-template junctions, XPA in nucleotide excision repair, and Rad52 in recombination — and acts at an early, pre-incision step of NER (PMID:1508673, PMID:1337880, PMID:10563794, PMID:12139939, PMID:12600993). The N-terminal domain coordinates checkpoint signaling by binding Rfc4, the MRN complex (MRE11/NBS1), and the ATR/Mec1 and Rad53/Chk2 apparatus, and RPA1 depletion activates the ATM-Chk2 axis (PMID:11340166, PMID:14642562, PMID:15620706, PMID:19586055). RPA1 function is tuned by writer/eraser-controlled post-translational modifications: cell-cycle phosphorylation by cdc2 and cyclin/Cdk2 kinases, PCAF/GCN5-mediated K163 acetylation (reversed by HDAC6/SIRT1) that drives XPA recruitment in NER, and CDYL-regulated crotonylation and HAT1-catalyzed K88 lactylation that promote ssDNA and MRN binding during homologous recombination (PMID:8308077, PMID:8798463, PMID:28854354, PMID:28854355, PMID:32201722, PMID:41271679, PMID:41694603). Beyond genome maintenance, RPA1 acts in transcriptional control, converting NRF2 into a repressor at ARE-NRE elements and promoting HNF4A activity and fatty-acid oxidation in liver, where its loss causes hepatic steatosis and hepatocellular carcinoma (PMID:30309964, PMID:35830798). In vivo, Rpa1 protects genomic integrity during development and immune homeostasis: deletion triggers cytosolic DNA leakage and ZBP1-RIPK3 necroptosis in T cells and cGAS-STING/ZBP1-driven PANoptosis in limb mesenchyme (PMID:36721037, PMID:40834068). Germline gain-of-function variants in DNA-binding domain A that hyperactivate ssDNA/telomeric binding cause severe telomere shortening and impaired hematopoiesis (PMID:34767620).

Mechanistic history

Synthesis pass · year-by-year structured walk · 24 steps
  1. 1990 High

    Established that RPA1 is an essential single-stranded DNA-binding protein required for DNA replication, defining its core cellular function.

    Evidence Gene cloning, homology analysis, and tetrad analysis of loss-of-function mutants in S. cerevisiae

    PMID:2192864

    Open questions at the time
    • Molecular mechanism of how ssDNA binding supports replication not yet defined
    • No biochemical reconstitution of the full complex
  2. 1992 High

    Placed RPA1 upstream of incision in nucleotide excision repair and physically linked it to the polymerase alpha-primase machinery, showing it functions beyond replication.

    Evidence Cell-free NER assays with neutralizing antibodies and pre-incised substrate bypass; far-Western binding to pol alpha-primase p48

    PMID:1337880 PMID:1508673

    Open questions at the time
    • Precise structural basis of NER role unresolved
    • Single-lab far-Western for p48 interaction
  3. 1993 High

    Resolved how RPA1 is cell-cycle regulated, distinguishing direct cdc2 phosphorylation at mitosis from indirect cdk2-dependent S-phase phosphorylation.

    Evidence Immunodepletion of Xenopus extracts and immunoprecipitation-kinase assays with purified cdk2 and cdc2

    PMID:8308077

    Open questions at the time
    • Functional consequence of each phosphorylation event not fully defined
    • Subunit specificity of phosphorylation incomplete
  4. 1996 High

    Clarified that cyclin A-Cdk2, not cyclin E-Cdk2, phosphorylates the RPA complex via direct cyclin A docking, refining the kinase specificity of RPA regulation.

    Evidence In vitro kinase and direct binding assays with purified cyclin-Cdk2 complexes

    PMID:8798463

    Open questions at the time
    • Phosphorylation maps mainly to RPA2 p34, not RPA1 directly
    • In vivo relevance not tested
  5. 1998 High

    Demonstrated genetically and physically that RPA1 functions in replication, repair, and recombination, and that it binds viral origin proteins via the RPA70 subunit.

    Evidence Yeast missense/ts mutant screen with recombination intermediate analysis; co-IP mapping of EBNA1 to RPA70

    PMID:9421526 PMID:9539419

    Open questions at the time
    • Mechanism distinguishing repair vs recombination defects unresolved
    • Viral origin interaction studies single lab
  6. 1999 High

    Provided structural basis for RPA1 protein-interaction function, revealing an N-terminal OB-fold beta-barrel with a basic cleft that binds acidic activator motifs and a cooperative XPA-binding surface for NER.

    Evidence NMR fold determination of RPA70 N-terminal domain; NMR chemical-shift mapping of XPA-MBD on RPA70

    PMID:10526407 PMID:10563794

    Open questions at the time
    • Full-length complex structure not resolved
    • How cleft selects among competing acidic partners unknown
  7. 2002 Medium

    Mapped a Rad52-binding domain on RPA1 and showed RPA-Rad52 interplay tunes ssDNA affinity and Rad52 oligomerization, linking RPA1 to recombination mechanics.

    Evidence Pulldown domain mapping and ssDNA-binding/self-association assays

    PMID:12139939

    Open questions at the time
    • Single-lab domain mapping
    • In vivo recombination contribution of this domain not tested
  8. 2003 Medium

    Identified a distinct primer-template junction recognition activity in the RPA70 C-terminal domain, separating junction binding from generic ssDNA binding.

    Evidence Photoaffinity crosslinking and limited proteolysis domain mapping

    PMID:12600993

    Open questions at the time
    • Functional role at forks not directly tested
    • Single-lab crosslinking
  9. 2001 High

    Connected the RPA1 N-terminal domain to checkpoint signaling through an allele-specific genetic interaction with Rfc4 in the DNA damage checkpoint.

    Evidence Synthetic lethality, HU/damage sensitivity, and checkpoint epistasis in yeast

    PMID:11340166

    Open questions at the time
    • Direct physical Rpa1N-Rfc4 contact not biochemically resolved
    • Mechanism of checkpoint signal transfer unclear
  10. 2003 Medium

    Established Mec1/ATR as a kinase acting on RPA1, mapping S178 phosphorylation in the N-terminal domain and linking it to Rad53 activation kinetics.

    Evidence Mec1 IP-kinase assay, site mapping, and rfa1-S178A mutant analysis in yeast

    PMID:14642562

    Open questions at the time
    • Functional consequence of S178 phosphorylation modest
    • Single-lab study
  11. 2005 High

    Demonstrated in vivo that RPA1 DNA-binding function suppresses chromosomal instability and tumorigenesis, with a dosage-sensitive role essential for embryogenesis.

    Evidence Mouse knock-in genetics, array CGH, MEF repair assays, and parallel yeast genetics

    PMID:15620706 PMID:15965476

    Open questions at the time
    • Pathway by which mutant RPA1 drives rearrangements not fully defined
    • Checkpoint placement (ATM-Chk2 vs Chk1) from siRNA is single-lab
  12. 2008 High

    Genetically separated RPA1's two functional modules — ssDNA binding versus the N-terminal damage-response domain — showing replication and repair impose distinct structural requirements.

    Evidence Depletion/replacement with systematic domain mutagenesis and functional readouts in human cells

    PMID:18469000

    Open questions at the time
    • Which damage-response partners require the N-terminus not enumerated here
    • Single rigorous study
  13. 2009 Medium

    Defined the RPA1 N-terminal OB-fold as the direct docking site for the MRN complex and showed RPA2 phosphorylation regulates this interaction during the damage response.

    Evidence Purified protein interaction assays, phosphomimetics, point/deletion mutagenesis, and cell-cycle analysis

    PMID:19586055

    Open questions at the time
    • Single-lab interaction study
    • Downstream MRN-dependent processing not mapped
  14. 2011 Medium

    Showed the RPA70 N-terminal domain uses an overlapping surface to bind Rad9 and p53, druggable by a small molecule that blocks these interactions without affecting ssDNA binding.

    Evidence HTS, competitive GST-fusion binding assays, helix-destabilization assay, and docking

    PMID:21459001

    Open questions at the time
    • Cellular efficacy of the inhibitor not established here
    • Single-lab biochemistry
  15. 2012 High

    Embedded RPA1 in checkpoint recruitment networks, showing it binds the Sgs1/BLM acidic domain to deliver Rad53 to stalled forks, and that p53-RPA70 dissociation in hypoxia frees RPA70 to enhance repair.

    Evidence Rpa70-binding site mapping on Sgs1 with Mec1/FHA1 assays; co-IP and p53 phosphosite mutagenesis with NER/NHEJ reporters

    PMID:22288499 PMID:22820947

    Open questions at the time
    • Direct structural detail of Sgs1-Rpa70 contact limited
    • Hypoxia p53-RPA70 study single-lab
  16. 2015 High

    Revealed RPA1 protein abundance at forks is actively regulated, with PTEN recruiting OTUB1 to deubiquitinate and accumulate RPA1, preventing fork collapse.

    Evidence Co-IP, STORM, iPOND, ubiquitination assays, and knockout phenotyping

    PMID:26403191

    Open questions at the time
    • Ubiquitin ligase opposing OTUB1 not identified here
    • Specific ubiquitination sites on RPA1 unmapped
  17. 2017 High

    Defined a writer/eraser-controlled acetylation code on RPA1, with PCAF/GCN5 K163 acetylation driving XPA recruitment in NER and HDAC6/SIRT1 reversing it.

    Evidence In vivo acetylation assays, K163 mutagenesis, NER and XPA-foci assays, and kinase/acetyltransferase activity assays; reported in two concurrent papers

    PMID:28854354 PMID:28854355

    Open questions at the time
    • Stoichiometry and dynamics of K163 acetylation in vivo not quantified
    • Crosstalk with phosphorylation not addressed
  18. 2018 Medium

    Expanded RPA1 into transcriptional regulation and identified additional damage-response regulators, showing it converts NRF2 to a repressor and is held off ATR by Ajuba.

    Evidence Co-IP, ChIP, RNA-seq, reporter and vascular assays for NRF2; co-IP and depletion assays for Ajuba

    PMID:29934626 PMID:30309964

    Open questions at the time
    • Both interactions single-lab
    • How RPA1 partitions between DNA-bound and transcriptional pools unknown
  19. 2020 Medium

    Established crotonylation as an HR-promoting modification on RPA1, controlled by CDYL and required for ssDNA and resection-machinery binding.

    Evidence Crotonylome proteomics, CDYL depletion, site mutagenesis, ssDNA binding and HR assays

    PMID:32201722

    Open questions at the time
    • Single-lab PTM study
    • Crotonyltransferase writer not identified here
  20. 2022 High

    Demonstrated that hyperactive ssDNA binding by RPA1 is pathogenic, with germline gain-of-function variants causing telomere shortening and impaired hematopoiesis.

    Evidence Biochemical ssDNA/telomeric binding assays, CRISPR knock-in iPSCs, and somatic genetic rescue analysis

    PMID:34767620

    Open questions at the time
    • Mechanism linking excess binding to telomere loss not fully resolved
    • Disease spectrum from these variants incompletely characterized
  21. 2022 Medium

    Identified RPA1 as a transcriptional/metabolic regulator in liver and as a target for a viral replication protein, broadening its non-replicative roles.

    Evidence Liver-specific Rpa1 knockout with ChIP and metabolic phenotyping; HBoV1 NP1 affinity/KD measurement and viral replication assay

    PMID:35653410 PMID:35830798

    Open questions at the time
    • Mechanistic link between HNF4A co-activation and DNA-binding function unclear
    • Both studies single-lab
  22. 2023 Medium

    Showed that loss of RPA1 genome protection triggers innate-immune cell death and that fork-bound RPA1 must be cleared by UBQLN1 to sustain replication and telomere integrity.

    Evidence T cell-specific Rpa1 knockout with ZBP1-RIPK3 and necroptosis-rescue experiments; UBQLN1 co-IP, fork analysis, and telomere length assays

    PMID:36721037 PMID:37463174

    Open questions at the time
    • Source/sensor link from RPA1 loss to cytosolic DNA not fully defined
    • UBQLN1 shuttling mechanism single-lab
  23. 2025 Medium

    Connected RPA1 to a lactylation modification and to developmental cell-death control, showing HAT1-mediated K88 lactylation promotes HR/radioresistance and Rpa1 loss drives cGAS-STING/ZBP1 PANoptosis.

    Evidence In vitro lactyltransferase assays, K88 mutagenesis, ssDNA/MRN binding and HR assays; limb-mesenchyme Rpa1 knockout with cGAS-STING/Z-DNA/PANoptosis analysis

    PMID:40834068 PMID:41271679 PMID:41694603

    Open questions at the time
    • Lactylation studies from a single research group
    • How RPA1 loss generates Z-DNA not mechanistically resolved
  24. 2025 Medium

    Provided quantitative single-cell dynamics of RPA1 ssDNA loading at stalled forks and showed ATR/ATM differentially control RPA accumulation versus exhaustion.

    Evidence Live-cell PCNA/RPA1 reporter imaging with ATR/ATM/MRE11 inhibitors

    PMID:39859385

    Open questions at the time
    • Molecular trigger of RPA exhaustion not defined
    • Single-lab imaging

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RPA1 partitions among its many roles — replication, repair, recombination, checkpoint, transcription, and innate-immune cell-death suppression — and how its dense PTM code is integrated to direct each function remains unresolved.
  • No unified model coordinating PTM writers/erasers with functional outcomes
  • Mechanism linking RPA1 loss to cytosolic/Z-DNA accumulation and innate immune sensing undefined
  • Structural basis for transcriptional (NRF2/HNF4A) versus DNA-binding functions unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 3 GO:0060090 molecular adaptor activity 3 GO:0140097 catalytic activity, acting on DNA 2 GO:0140110 transcription regulator activity 2
Localization
GO:0000228 nuclear chromosome 3 GO:0005634 nucleus 3
Pathway
R-HSA-73894 DNA Repair 4 R-HSA-8953897 Cellular responses to stimuli 4 R-HSA-69306 DNA Replication 3 R-HSA-5357801 Programmed Cell Death 2 R-HSA-74160 Gene expression (Transcription) 2
Partners
HNF4AMRE11NBS1NFE2L2PTENRAD52TP53XPA
Complex memberships
MRN complex (interacting)NRF2-RPA1 transcriptional complexRPA heterotrimer

Evidence

Reading pass · 37 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1990 S. cerevisiae RPA1 encodes an essential single-stranded DNA binding protein homologous to the 70 kDa subunit of human RP-A; loss of RPA1 causes accumulation of multiply-budded cells with a single nucleus, indicating a defect in DNA replication. Gene cloning, sequence homology analysis, tetrad analysis of heterozygous insertion mutants The EMBO journal High 2192864
1992 HSSB/RPA1 functions at an early stage of nucleotide excision repair (NER), prior to repair synthesis; anti-HSSB antibodies that inhibit DNA polymerase alpha stimulation also inhibit NER synthesis in cell-free extracts, and when damaged DNA is pre-incised by UvrABC the requirement for HSSB is bypassed, placing RPA1 upstream of the incision step. Cell-free NER assay with neutralizing antibodies; rescue by pre-incised substrate Nucleic acids research High 1508673
1992 Bovine RP-A (RPA1/p70 subunit) directly interacts with the p48 subunit of DNA polymerase alpha-primase complex, as demonstrated by modified blotting (far-Western); bovine RP-A can substitute for human RP-A in SV40 DNA replication in vitro. Modified blotting (far-Western), in vitro SV40 replication assay Chromosoma Medium 1337880
1993 RP-A is essential for chromosomal DNA replication in Xenopus cell-free extracts (immunodepletion causes replication failure). During S phase, only nucleus-associated and single-stranded DNA-bound RPA is phosphorylated, and cdk2 is required for this S-phase phosphorylation indirectly (cdk2 depletion blocks replication before RPA binds ssDNA). At mitosis onset, cdc2 kinase directly phosphorylates RP-A independently of DNA binding. Immunodepletion from Xenopus extracts; immunoprecipitation-kinase assays with purified cdk2 and cdc2 Journal of cell science High 8308077
1995 The RPA70 (RPA1) subunit physically associates with the EBNA-2 acidic transactivation domain; a Trp-to-Thr mutation in EBNA-2 that abolishes transactivation also disrupts the EBNA-2–RPA70 interaction, genetically linking RPA70 binding to transcriptional activation. Co-immunoprecipitation/association assay; mutational analysis of EBNA-2 Journal of virology Medium 7983760
1996 Cyclin A-Cdk2, but not cyclin E-Cdk2, efficiently phosphorylates the p34 subunit of HSSB (RPA2) in the heterotrimeric RPA complex; this differential phosphorylation is driven by a direct interaction between cyclin A and HSSB-p34, not cyclin E. The large subunit RPA70 (RPA1) is present in this complex. In vitro kinase assays with purified cyclin-Cdk2 complexes; direct binding assays between cyclin A/E and HSSB-p34 The Journal of biological chemistry High 8798463
1996 Transcription positive cofactor 4 (PC4), a ssDNA-binding protein, forms complexes with HSSB/RPA on ssDNA and inhibits RPA-dependent RNA primer synthesis and DNA polymerase delta-catalyzed chain elongation in SV40 replication; excess HSSB/RPA reverses these inhibitory effects. In vitro SV40 DNA replication assay; biochemical complex formation on ssDNA The Journal of biological chemistry High 8703021
1998 Yeast RPA1 (RFA1) has multiple in vivo functions in DNA replication, repair, and recombination; specific missense mutations cause UV/MMS sensitivity and defects in mating-type switching and single-strand annealing recombination, while temperature-sensitive alleles arrest replication. Most mutations alter conserved residues also found in human and Xenopus RPA1. Genetic mutant screen; physical analysis of recombination intermediates; cell cycle analysis Genetics High 9539419
1998 Human RPA (hSSB) directly interacts with EBNA1, the latent origin binding protein of EBV; the main contact is via the 70 kDa subunit (RPA1/RPA70), and this interaction occurs both in solution and when EBNA1 is bound to the EBV origin, suggesting RPA1 participates in latent EBV origin activation. Co-immunoprecipitation; binding assays in solution and on origin DNA Nucleic acids research Medium 9421526
1999 NMR structure of the N-terminal domain of RPA70 (residues 8–108) reveals a five-stranded anti-parallel beta-barrel with a large basic cleft formed by two loops; this cleft is the likely binding site for acidic motifs of transcriptional activators including p53. Lethal or conditional-lethal yeast point mutants map to this cleft. NMR global fold determination; mapping of known yeast mutants onto structure Journal of biomolecular NMR High 10526407
1999 XPA minimal DNA-binding domain contacts RPA70 (RPA1) residues spanning both the zinc-binding and loop-rich subdomains, with partial overlap with the DNA-binding surface, suggesting cooperative DNA-binding between XPA and RPA70 in NER. DNA binding by XPA is localized to the loop-rich subdomain regardless of lesion type. NMR chemical shift mapping; 15N relaxation studies of XPA-MBD bound to RPA70ΔC327 Biochemistry High 10563794
2001 The N-terminal domain of Rpa1 (Rpa1N) physically interacts with Rfc4, a small subunit of replication factor C; rfa1 mutations in Rpa1N that confer hydroxyurea sensitivity are lethal when combined with rfc4-2. Both rfc4-2 and rfa1-t11 strains are defective in G2/M DNA damage checkpoint, and RFC4 is epistatic with RAD24 for DNA damage sensitivity, placing Rpa1N-Rfc4 interaction in checkpoint signaling. Genetic interaction (synthetic lethality), hydroxyurea/DNA damage sensitivity assays, checkpoint epistasis analysis Molecular and cellular biology High 11340166
2002 Human RPA forms a complex with Rad52 through two binding sites: the C-terminal domain of RPA32 (residues 224–271) and a newly identified domain on RPA70 (residues 169–326 of RPA1). Rad52 binding to RPA increases ssDNA affinity significantly; RPA binding to Rad52 inhibits higher-order self-association of Rad52 rings. DBD-D on RPA32 is primarily responsible for ssDNA-binding enhancement. Pulldown assays with defined protein fragments; ssDNA binding assays; analysis of Rad52 self-association Journal of molecular biology Medium 12139939
2003 Human RPA70 (RPA1) C-terminal domain (residues 432–616) and the central domain of RPA32 (residues 39–180) interact with the 3'-end of a primer at a primer-template junction, as shown by photoaffinity labeling and limited proteolysis; this interaction is distinct from ssDNA binding. Photoaffinity crosslinking; limited proteolysis; domain mapping The Journal of biological chemistry Medium 12600993
2003 Yeast Mec1 kinase physically associates with RPA and phosphorylates both RPA1 and RPA2 in vitro; the major phosphorylation site on RPA1 maps to serine 178 in the N-terminal domain (RPA1N). The phosphorylation-defective rfa1-S178A mutant shows reduced physical interaction with Mec1 and altered kinetics of RPA1 and Rad53 phosphorylation in vivo. Mec1 immunoprecipitation-kinase assay; in vitro phosphorylation with site mapping; rfa1-S178A mutant phenotypic analysis DNA repair Medium 14642562
2005 A heterozygous missense mutation in the DNA-binding domain of mouse Rpa1 causes defects in DNA double-strand break repair, chromosomal breaks, aneuploidy in primary MEFs, and leads to lymphoid tumors with large-scale chromosomal rearrangements. Homozygous mutation causes early embryonic lethality. The equivalent yeast mutation is hypomorphic, semidominant, and enhances gross chromosomal rearrangements. Mouse knock-in genetics; array CGH; MEF DNA repair assays; yeast genetic analysis Nature genetics High 15965476
2005 siRNA knockdown of RPA1 in HeLa cells activates ATM (Ser1981) and Chk2 (Thr68) phosphorylation and upregulates p21, but not Chk1 (Ser345); ATM inhibitor caffeine and ATM siRNA both block Chk2 phosphorylation induced by RPA1 loss, placing RPA1 upstream of the ATM-Chk2 checkpoint axis. siRNA knockdown; immunoblotting for checkpoint kinase phosphorylation; ATM inhibitor and siRNA epistasis FEBS letters Medium 15620706
2008 RPA1 contains four structurally related OB-fold domains; mutations that substantially decrease ssDNA binding do not necessarily disrupt cellular function, while mutations that only slightly affect ssDNA binding can dramatically affect DNA repair and cell cycle progression. The N-terminus of RPA1 is dispensable for DNA replication but required for the cellular DNA damage response. Conserved aromatic residues in high-affinity ssDNA-binding domains are essential for DNA repair. Depletion/replacement strategy in human cells; site-directed mutagenesis of individual domains; functional readouts for replication, repair, and cell cycle The Journal of biological chemistry High 18469000
2009 RPA1 N-terminal OB-fold domain directly interacts with MRN complex (MRE11 and NBS1); deletion of the N-terminal OB-fold or substitution of Arg31/Arg41 with alanines abrogates RPA-MRN interaction. RPA2 phosphorylation weakens the RPA-MRN interaction. Disruption of RPA-MRN binding alters cell cycle progression after DNA damage. Purified protein interaction assays; RPA2 phosphomimetic constructs; deletion and point mutagenesis of RPA1 N-terminus; cell cycle analysis Biochemistry Medium 19586055
2011 RPA70 N-terminal domain (DBD) binds Rad9 and p53 peptides at an overlapping surface; small molecule NSC15520 competitively inhibits binding of both Rad9-GST and p53-GST to RPA70 N-terminal DBD with IC50 ~10 μM, and inhibits RPA70 N-terminal domain-dependent helix destabilization activity, without affecting ssDNA binding. High-throughput screening; competitive binding assays with GST-fusion proteins; DNA helix-destabilization assay; computational docking Bioorganic & medicinal chemistry Medium 21459001
2012 Sgs1 (BLM ortholog) N-terminal acidic domain (R1 domain) directly interacts with Rpa70 (Rpa1); deletion of R1 reduces Rad53 activation on hydroxyurea. Mec1 kinase phosphorylates the Sgs1 R1 domain, which promotes high-affinity binding to the Rad53 FHA1 domain, recruiting Rad53 to stalled forks for checkpoint activation. Identification and deletion of Rpa70-binding site on Sgs1; Mec1 kinase assay; FHA1 binding assay; epistasis with repair genes The EMBO journal High 22820947
2012 In hypoxia, DNA-PK phosphorylates p53 at Ser15, which dissociates the p53-RPA70 (RPA1) complex; free RPA70 then enhances NER/NHEJ repair, and RPA70 gene silencing significantly increases apoptosis in hypoxic cancer cells. Co-immunoprecipitation; site-directed mutagenesis of p53 Ser/Thr residues; RPA70 gene silencing; NER/NHEJ reporter assays The Biochemical journal Medium 22288499
2015 PTEN physically associates with RPA1 via the RPA1 C-terminal domain; PTEN promotes RPA1 accumulation at replication forks (shown by iPOND) and recruits the deubiquitinase OTUB1 to mediate RPA1 deubiquitination. PTEN deletion leads to replication fork collapse, and RPA1 deletion phenocopies PTEN knockout in terms of fork stalling. Co-immunoprecipitation; STORM imaging; iPOND; ubiquitination assays; domain-mapping; knockout cell phenotyping Cell research High 26403191
2017 PCAF/GCN5 acetyltransferases acetylate RPA1 at K163; this acetylation is required for NER but not for other repair pathways. Acetylation at K163 promotes stable XPA accumulation at UV-damaged DNA. DNA-PK phosphorylates and activates PCAF upon UV damage to drive RPA1 K163 acetylation. HDAC6 and SIRT1 deacetylate RPA1 K163, and UV causes HDAC6 cytoplasmic translocation, increasing RPA1 acetylation. In vivo acetylation assays; site-directed mutagenesis (K163); NER assays; XPA foci quantification; kinase and acetyltransferase activity assays Cell reports High 28854354 28854355
2018 RPA1 binds to NRF2 and competes with sMAF proteins for NRF2 binding; the NRF2-RPA1 complex binds a specific ARE-NRE sequence to repress transcription of target genes, converting NRF2 from a transcriptional activator to a repressor. This was shown to repress MYLK expression and disrupt vascular integrity. Co-immunoprecipitation; ChIP; RNA-seq; genome-wide ARE-NRE analysis; reporter assays; vascular permeability assays in lung injury model Proceedings of the National Academy of Sciences of the United States of America Medium 30309964
2018 LIM protein Ajuba directly and physically interacts with RPA70 (RPA1); this interaction is cell cycle-regulated and reduced upon DNA replication stress. Depletion of Ajuba leads to potent ATR activation, consistent with Ajuba acting as a negative regulator of ATR by occupying RPA70. Co-immunoprecipitation; direct binding assay; cell cycle synchronization; Ajuba depletion and ATR activation readouts Scientific reports Medium 29934626
2020 CDYL negatively regulates crotonylation (Kcr) of RPA1; mutation of RPA1 crotonylation sites impairs its interaction with single-stranded DNA and with components of the DNA resection machinery, demonstrating that RPA1 Kcr is required for homologous recombination DNA repair. Quantitative proteomics crotonylome; CDYL depletion; site mutagenesis; ssDNA binding assay; HR repair assay Science advances Medium 32201722
2022 Gain-of-function germline missense variants in RPA1 (E240K and V227A) in DNA-binding domain A cause increased binding to single-stranded and telomeric DNA, leading to severe telomere shortening and impaired hematopoietic differentiation. CRISPR/Cas9 knock-in of RPA1E240K in iPSCs recapitulates telomere shortening, establishing that hyperactive ssDNA binding by RPA1 disrupts telomere maintenance. Biochemical ssDNA/telomeric DNA binding assays; CRISPR/Cas9 knock-in; iPSC hematopoietic differentiation; somatic genetic rescue analysis Blood High 34767620
2022 RPA1 binds gene regulatory regions, chromatin-remodeling factors, and HNF4A in liver cells; RPA1 promotes HNF4A transcriptional activity and fatty acid β-oxidation. Liver-specific deletion of Rpa1 in mice causes downregulation of fatty acid oxidation genes, hepatic steatosis, and hepatocellular carcinoma. Rpa1+/- mice develop fatty liver during aging and on high-fat diet. Conditional liver-specific Rpa1 knockout; ChIP; chromatin remodeling factor interaction assays; gene expression analysis; metabolic phenotyping Cell reports Medium 35830798
2023 T cell-specific deletion of Rpa1 (Rpa1fl/fl Cd4-cre mice) causes peripheral T cell lymphopenia and reduced TCR repertoire diversity. Mechanistically, RPA1 depletion causes genomic DNA leakage into the cytosol, activating ZBP1-RIPK3 signaling and T cell necroptosis; chemical or genetic inhibition of necroptosis rescues the phenotype. Conditional T cell-specific Rpa1 knockout; ZBP1-RIPK3 pathway analysis; cytosolic DNA detection; necroptosis inhibition rescue experiments Advanced science Medium 36721037
2025 Conditional knockout of Rpa1 in limb bud mesenchyme causes near-total absence of forelimbs; mechanistically, Rpa1 deletion leads to extensive DNA damage, activates the cGAS-STING pathway driving Zbp1 transcription, and causes Z-DNA accumulation that activates ZBP1-dependent PANoptosis (combined apoptosis, necroptosis, pyroptosis) in mesenchymal stem cells. Conditional Rpa1 knockout in limb mesenchyme; cGAS-STING pathway assays; Z-DNA immunostaining; PANoptosis markers; genetic epistasis with ZBP1 Science advances Medium 40834068
2025 HAT1 acts as a lactyltransferase that directly catalyzes lactylation of RPA1 at K88 (and K15 of RPA1 in lung adenocarcinoma study); RPA1 lactylation at K88 facilitates RPA1 binding to ssDNA and to MRN complexes, promotes homologous recombination, and confers radioresistance. HAT1 knockout inhibits DNA repair and increases radiosensitivity. In vitro lactyltransferase assay; site-directed mutagenesis (K88); ssDNA binding assay; MRN co-immunoprecipitation; HR repair assay; HAT1 KO in vitro and in vivo Cell death & disease / International journal of biological sciences Medium 41271679 41694603
2025 Live-cell imaging reveals that RPA1 accumulates on ssDNA at stalled replication forks (up to ~2400 nt per fork) during nucleotide depletion; ATR inhibition accelerates RPA1 accumulation ~9-fold leading to RPA exhaustion, while ATM inhibition with ATR inhibition causes persistence of ~2500 nt ssDNA per fork post-S-phase and mitotic catastrophe. MRE11 inhibition does not affect PCNA/RPA1 dynamics. Live-cell fluorescence microscopy (PCNA/RPA1 reporters); single-cell tracking at 30-second resolution; ATR/ATM inhibitors; MRE11 inhibition International journal of molecular sciences Medium 39859385
2023 UBQLN1 interacts with RPA1 and shuttles it off the replication fork; UBQLN1 deficiency retains RPA1 at replication forks, hinders replication, and causes cell cycle arrest, genome instability, and rapid telomere shortening (particularly at G-rich telomeric sequences). This leads to lung fibrosis in mice. Co-immunoprecipitation; replication fork analysis; telomere length assay; UBQLN1 depletion in cells and mice PLoS genetics Medium 37463174
2024 CDC20 stabilizes RPA1 protein through direct protein-protein interaction in early stages of DNA damage, activating ATR-mediated signaling; in later stages, CDC20 promotes ubiquitin-mediated degradation of RPA1 to complete DNA repair. CDC20 promotes homologous recombination and radioresistance. Co-immunoprecipitation; HR and NHEJ reporter systems; ubiquitination assay; tumor xenograft International journal of molecular sciences Low 39125953
2022 HBoV1 NP1 protein directly interacts with RPA70 (RPA1) at domain aa181–422 with high affinity (KD ~122 nM); this NP1-RPA70 interaction is required for HBoV1 DNA replication in vitro and in infected human airway epithelium cultures. Affinity purification; equilibrium dissociation constant measurement; domain mapping; dominant-negative strategy in viral DNA replication assay PLoS pathogens Medium 35653410
2025 USP49 deubiquitinase, recruited to DNA DSBs via ATM-promoted liquid-liquid phase separation, deubiquitinates and stabilizes RPA70 (RPA1) at DSBs along with Rad51, thereby promoting homologous recombination repair and radioresistance. Co-immunoprecipitation; ubiquitination/deubiquitination assay; LLPS analysis; HR reporter; tumor xenograft International journal of biological macromolecules Low 40460957

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1998 NHE-RF, a regulatory cofactor for Na(+)-H+ exchange, is a common interactor for merlin and ERM (MERM) proteins. The Journal of biological chemistry 206 9430655
1990 An essential Saccharomyces cerevisiae single-stranded DNA binding protein is homologous to the large subunit of human RP-A. The EMBO journal 188 2192864
1998 Genetic analysis of yeast RPA1 reveals its multiple functions in DNA metabolism. Genetics 166 9539419
1992 A role for the human single-stranded DNA binding protein HSSB/RPA in an early stage of nucleotide excision repair. Nucleic acids research 151 1508673
2017 CPPred-RF: A Sequence-based Predictor for Identifying Cell-Penetrating Peptides and Their Uptake Efficiency. Journal of proteome research 140 28436664
1993 Distinct roles of cdk2 and cdc2 in RP-A phosphorylation during the cell cycle. Journal of cell science 132 8308077
2005 Mutation in Rpa1 results in defective DNA double-strand break repair, chromosomal instability and cancer in mice. Nature genetics 127 15965476
2008 Comparative genome analysis of "Candidatus Phytoplasma australiense" (subgroup tuf-Australia I; rp-A) and "Ca. Phytoplasma asteris" Strains OY-M and AY-WB. Journal of bacteriology 123 18359806
2020 Global crotonylome reveals CDYL-regulated RPA1 crotonylation in homologous recombination-mediated DNA repair. Science advances 120 32201722
2013 SFCscore(RF): a random forest-based scoring function for improved affinity prediction of protein-ligand complexes. Journal of chemical information and modeling 112 23705795
2008 Cellular functions of human RPA1. Multiple roles of domains in replication, repair, and checkpoints. The Journal of biological chemistry 96 18469000
2001 Rfc4 interacts with Rpa1 and is required for both DNA replication and DNA damage checkpoints in Saccharomyces cerevisiae. Molecular and cellular biology 95 11340166
1995 The Epstein-Barr virus nuclear protein 2 acidic domain can interact with TFIIB, TAF40, and RPA70 but not with TATA-binding protein. Journal of virology 91 7983760
2017 PhosPred-RF: A Novel Sequence-Based Predictor for Phosphorylation Sites Using Sequential Information Only. IEEE transactions on nanobioscience 90 28166503
1999 Human replication protein A: global fold of the N-terminal RPA-70 domain reveals a basic cleft and flexible C-terminal linker. Journal of biomolecular NMR 83 10526407
2001 NHE-RF, a merlin-interacting protein, is primarily expressed in luminal epithelia, proliferative endometrium, and estrogen receptor-positive breast carcinomas. The American journal of pathology 82 11141479
2015 PTEN regulates RPA1 and protects DNA replication forks. Cell research 80 26403191
1999 Interactions of human nucleotide excision repair protein XPA with DNA and RPA70 Delta C327: chemical shift mapping and 15N NMR relaxation studies. Biochemistry 77 10563794
2012 An N-terminal acidic region of Sgs1 interacts with Rpa70 and recruits Rad53 kinase to stalled forks. The EMBO journal 71 22820947
1987 Structural analysis of the Drosophila rpA1 gene, a member of the eucaryotic 'A' type ribosomal protein family. Nucleic acids research 70 3103101
2017 PCAF/GCN5-Mediated Acetylation of RPA1 Promotes Nucleotide Excision Repair. Cell reports 67 28854354
2008 Immunolocalization of Kim-1, RPA-1, and RPA-2 in kidney of gentamicin-, mercury-, or chromium-treated rats: relationship to renal distributions of iNOS and nitrotyrosine. Toxicologic pathology 62 18441258
2002 Analysis of the human replication protein A:Rad52 complex: evidence for crosstalk between RPA32, RPA70, Rad52 and DNA. Journal of molecular biology 60 12139939
2018 RPA1 binding to NRF2 switches ARE-dependent transcriptional activation to ARE-NRE-dependent repression. Proceedings of the National Academy of Sciences of the United States of America 53 30309964
2023 Bitter-RF: A random forest machine model for recognizing bitter peptides. Frontiers in medicine 51 36778738
2022 Gain-of-function mutations in RPA1 cause a syndrome with short telomeres and somatic genetic rescue. Blood 51 34767620
2009 Physical interaction between replication protein A (RPA) and MRN: involvement of RPA2 phosphorylation and the N-terminus of RPA1. Biochemistry 51 19586055
1996 Transcription-positive cofactor 4 forms complexes with HSSB (RPA) on single-stranded DNA and influences HSSB-dependent enzymatic synthesis of simian virus 40 DNA. The Journal of biological chemistry 51 8703021
2017 Ailanthone inhibits non-small cell lung cancer cell growth through repressing DNA replication via downregulating RPA1. British journal of cancer 48 29024939
1992 Purification and functional characterization of bovine RP-A in an in vitro SV40 DNA replication system. Chromosoma 48 1337880
2017 UV-Induced RPA1 Acetylation Promotes Nucleotide Excision Repair. Cell reports 46 28854355
1998 Human RPA (hSSB) interacts with EBNA1, the latent origin binding protein of Epstein-Barr virus. Nucleic acids research 46 9421526
1997 Expression of an ortholog of replication protein A1 (RPA1) is induced by gibberellin in deepwater rice. Proceedings of the National Academy of Sciences of the United States of America 46 9275237
2004 Reduced pigmentation (rp), a mouse model of Hermansky-Pudlak syndrome, encodes a novel component of the BLOC-1 complex. Blood 43 15265785
2003 Human replication protein A. The C-terminal RPA70 and the central RPA32 domains are involved in the interactions with the 3'-end of a primer-template DNA. The Journal of biological chemistry 43 12600993
2019 k-Skip-n-Gram-RF: A Random Forest Based Method for Alzheimer's Disease Protein Identification. Frontiers in genetics 42 30809242
2011 Small molecule inhibitor of the RPA70 N-terminal protein interaction domain discovered using in silico and in vitro methods. Bioorganic & medicinal chemistry 36 21459001
2009 Differences in immunolocalization of Kim-1, RPA-1, and RPA-2 in kidneys of gentamicin-, cisplatin-, and valproic acid-treated rats: potential role of iNOS and nitrotyrosine. Toxicologic pathology 34 19535489
2012 PROTS-RF: a robust model for predicting mutation-induced protein stability changes. PloS one 33 23077576
2003 MEC1-dependent phosphorylation of yeast RPA1 in vitro. DNA repair 33 14642562
1987 PAF binding sites. Characterization by [3H]52770 RP, a pyrrolo[1,2-c]thiazole derivative, in rabbit platelets. Biochemical pharmacology 33 2822048
2021 iTTCA-RF: a random forest predictor for tumor T cell antigens. Journal of translational medicine 32 34706730
1996 Studies on the in vitro phosphorylation of HSSB-p34 and -p107 by cyclin-dependent kinases. Cyclin-substrate interactions dictate the efficiency of phosphorylation. The Journal of biological chemistry 29 8798463
1985 Regulation of transcription of the repA1 gene in the replication control region of IncFII plasmid NR1 by gene dosage of the repA2 transcription repressor protein. Journal of bacteriology 29 3155722
2012 p53 Ser15 phosphorylation disrupts the p53-RPA70 complex and induces RPA70-mediated DNA repair in hypoxia. The Biochemical journal 28 22288499
1992 Expression of the repA1 gene of IncFII plasmid NR1 is translationally coupled to expression of an overlapping leader peptide. Journal of bacteriology 28 1447133
2011 Analyses of Candida Cdc13 orthologues revealed a novel OB fold dimer arrangement, dimerization-assisted DNA binding, and substantial structural differences between Cdc13 and RPA70. Molecular and cellular biology 27 22025677
1997 Cell cycle regulation of RPA1 transcript levels in the trypanosomatid Crithidia fasciculata. Nucleic acids research 27 9241242
2001 TIN-ag-RP, a novel catalytically inactive cathepsin B-related protein with EGF domains, is predominantly expressed in vascular smooth muscle cells. Biochemistry 26 11170462
1988 [3H]52770 RP, a platelet-activating factor receptor antagonist, and tritiated platelet-activating factor label a common specific binding site in human polymorphonuclear leukocytes. The Journal of pharmacology and experimental therapeutics 26 2831350
2016 miR-30a can inhibit DNA replication by targeting RPA1 thus slowing cancer cell proliferation. The Biochemical journal 25 27208176
2018 Differential RPA-1 and RAD-51 recruitment in vivo throughout the C. elegans germline, as revealed by laser microirradiation. Nucleic acids research 24 29244155
2013 Trends in immediate perioperative morbidity and delay in discharge after open and minimally invasive radical prostatectomy (RP): a 20-year institutional experience. BJU international 23 23759008
2011 Increased RPA1 gene dosage affects genomic stability potentially contributing to 17p13.3 duplication syndrome. PLoS genetics 23 21901111
2005 Ginsenoside Rf, a component of ginseng, regulates lipoprotein metabolism through peroxisome proliferator-activated receptor alpha. Biochemical and biophysical research communications 23 16297877
2018 Oncogene RPA1 promotes proliferation of hepatocellular carcinoma via CDK4/Cyclin-D pathway. Biochemical and biophysical research communications 22 29477843
2018 RPA1 downregulation enhances nasopharyngeal cancer radiosensitivity via blocking RAD51 to the DNA damage site. Experimental cell research 22 30144445
2022 The small nonstructural protein NP1 of human bocavirus 1 directly interacts with Ku70 and RPA70 and facilitates viral DNA replication. PLoS pathogens 21 35653410
2023 Loss of RPA1 Impairs Peripheral T Cell Homeostasis and Exacerbates Inflammatory Damage through Triggering T Cell Necroptosis. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20 36721037
2020 Rpa1 mediates an immune response to avrRpm1Psa and confers resistance against Pseudomonas syringae pv. actinidiae. The Plant journal : for cell and molecular biology 19 31849122
1998 Nuleclear extracts of Crithidia fasciculata contain a factor(s) that binds to the 5'-untranslated regions of TOP2 and RPA1 mRNAs containing sequences required for their cell cycle regulation. The Journal of biological chemistry 19 9726980
2022 RPA1 controls chromatin architecture and maintains lipid metabolic homeostasis. Cell reports 18 35830798
2020 Radioresistance, DNA Damage and DNA Repair in Cells With Moderate Overexpression of RPA1. Frontiers in genetics 18 32849834
2019 Genetic variants in RPA1 associated with the response to oxaliplatin-based chemotherapy in colorectal cancer. Journal of gastroenterology 18 30923916
2013 The natural absence of RPA1N domain did not impair Leishmania amazonensis RPA-1 participation in DNA damage response and telomere protection. Parasitology 18 23388105
1994 High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3). Genomics 17 8020972
2012 Physical and functional interactions of Caenorhabditis elegans WRN-1 helicase with RPA-1. Biochemistry 16 22257160
2016 RpA1 ameliorates symptoms of mutant ataxin-1 knock-in mice and enhances DNA damage repair. Human molecular genetics 15 28173122
2014 RPA-1 from Leishmania amazonensis (LaRPA-1) structurally differs from other eukaryote RPA-1 and interacts with telomeric DNA via its N-terminal OB-fold domain. FEBS letters 14 25451229
2005 Loss of RPA1 induces Chk2 phosphorylation through a caffeine-sensitive pathway. FEBS letters 13 15620706
1993 Mapping of the 70 kDa, 34 kDa, and 11 kDa subunit genes of the human multimeric single-stranded DNA binding protein (hSSB/RPA) to chromosome bands 17p13, 1p35-p36.1, and 7p21-p22. Cell structure and function 12 8293499
2020 A multi-approach analysis highlights the relevance of RPA-1 as a telomere end-binding protein (TEBP) in Leishmania amazonensis. Biochimica et biophysica acta. General subjects 11 32222548
2015 RPA1 expression in esophageal carcinoma and its influence on radiosensitivity of esophageal carcinoma TE-1 cells. Panminerva medica 11 26824734
2015 RPA70 depletion induces hSSB1/2-INTS3 complex to initiate ATR signaling. Nucleic acids research 9 25916848
1997 Role of the RepA1 protein in RepFIC plasmid replication. Journal of bacteriology 9 9079900
1991 Isolation and properties of the RepA1 protein of the IncFII replicon, RepFIC. Molecular microbiology 8 1857211
2021 CWLy-RF: A novel approach for identifying cell wall lyases based on random forest classifier. Genomics 7 34186189
2020 Replication Protein A (RPA1, RPA2 and RPA3) expression in gastric cancer: correlation with clinicopathologic parameters and patients' survival. Journal of B.U.ON. : official journal of the Balkan Union of Oncology 7 32862594
2025 RPA1 protects DNA damage-induced PANoptosis in limb development. Science advances 6 40834068
2018 LIM Protein Ajuba associates with the RPA complex through direct cell cycle-dependent interaction with the RPA70 subunit. Scientific reports 6 29934626
2017 Ebulin-RP, a novel member of the Ebulin gene family with low cytotoxicity as a result of deficient sugar binding domains. Biochimica et biophysica acta. General subjects 6 29154940
2024 The DNA double-strand break repair proteins γH2AX, RAD51, BRCA1, RPA70, KU80, and XRCC4 exhibit follicle-specific expression differences in the postnatal mouse ovaries from early to older ages. Journal of assisted reproduction and genetics 5 39023827
2001 DNA double-strand breaks induce formation of RP-A/Ku foci on in vitro reconstituted Xenopus sperm nuclei. Journal of cell science 5 11591822
1997 Isolation and characterization of replication protein A (RP-A) from tobacco cells. FEBS letters 5 9287140
2023 UBQLN1 deficiency mediates telomere shortening and IPF through interacting with RPA1. PLoS genetics 4 37463174
2023 Germline landscape of RPA1, RPA2 and RPA3 variants in pediatric malignancies: identification of RPA1 as a novel cancer predisposition candidate gene. Frontiers in oncology 4 37869077
1993 Suppression of replication-deficient mutants of IncFII plasmid NR1 can occur by two different mechanisms that increase expression of the repA1 gene. Journal of bacteriology 4 8491730
2025 In and out of Replication Stress: PCNA/RPA1-Based Dynamics of Fork Stalling and Restart in the Same Cell. International journal of molecular sciences 3 39859385
2025 USP49 undergoes liquid-liquid phase separation and stabilizes RPA70 to induce radioresistance through homologous recombination repair in esophageal squamous cell carcinoma. International journal of biological macromolecules 3 40460957
2024 Cinchophen induces RPA1 related DNA damage and apoptosis to impair ENS development of zebrafish. Ecotoxicology and environmental safety 3 38306819
2023 Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeast. PLoS genetics 3 37200372
1997 Interactions of the RepA1 protein with its replicon targets: two opposing roles in control of plasmid replication. Journal of bacteriology 3 9190795
1996 Crystallization and preliminary crystallographic analysis of RepA1, a replication control protein of the RepFIC replicon of enterotoxin plasmid EntP307. Proteins 3 8727327
2025 Circular RNA circLIMK1-005 promotes the progression of lung adenocarcinoma by interacting with RPA1 protein to activate CDK4 signaling. Cell death discovery 1 40593477
2025 HAT1 functions as a lactyltransferase and mediates RPA1 lactylation to promote DNA repair and radioresistance in lung adenocarcinoma. Cell death & disease 1 41271679
2024 CDC20 Holds Novel Regulation Mechanism in RPA1 during Different Stages of DNA Damage to Induce Radio-Chemoresistance. International journal of molecular sciences 1 39125953
2024 Exploring RPA1-ETAA1 axis via high-throughput data analysis: implications for PD-L1 nuclear translocation and tumor-immune dynamics in liver cancer. Frontiers in immunology 1 39660144
2021 Remote Homology Detection Identifies a Eukaryotic RPA DBD-C-like DNA Binding Domain as a Conserved Feature of Archaeal Rpa1-Like Proteins. Frontiers in molecular biosciences 1 34355021
1999 Molecular cloning, developmental expression, and cellular localization of the 70-kDa RPA-1 subunit of Drosophila melanogaster. DNA and cell biology 1 10619604
2026 HAT1 as a Lactyltransferase to Promote DNA Repair Through RPA1 Lactylation in Glioblastoma. International journal of biological sciences 0 41694603

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