Affinage

RMI1

RecQ-mediated genome instability protein 1 · UniProt Q9H9A7

Length
625 aa
Mass
70.1 kDa
Annotated
2026-04-28
65 papers in source corpus 35 papers cited in narrative 36 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RMI1 is a conserved OB-fold scaffolding protein that functions as an essential subunit of the BLM–Topoisomerase IIIα–RMI1–RMI2 (BTRR/dissolvasome) complex, coupling RecQ helicase-driven unwinding to topoisomerase III strand-passage activity to dissolve double Holliday junctions, hemicatenanes, and D-loops into non-crossover products, thereby suppressing sister chromatid exchanges and maintaining genome stability (PMID:15775963, PMID:16537486, PMID:25699708). Structurally, RMI1 attaches to the DNA-passing gate of Topo IIIα via its N-terminal OB-fold domain and projects a loop into the gate that stabilizes the open covalent intermediate, accelerating the rate-limiting ssDNA-trapping step and shifting the enzyme's activity from supercoil relaxation toward decatenation and dissolution (PMID:24509834, PMID:22885009, PMID:40266687). Beyond dissolution, RMI1 stimulates BLM-mediated DNA unwinding to promote processive DNA end resection with DNA2 and orients BLM for efficient D-loop disruption, and it is recruited to stalled replication forks through RNF8-mediated K63-polyubiquitylation at specific lysines, linking its functions to replication fork recovery (PMID:25200081, PMID:35115525, PMID:41784835). During meiosis and mitosis, the Top3–Rmi1 module acts both with and independently of Sgs1/BLM to resolve chromosome entanglements, and its activity is modulated by Smc5/6-mediated sumoylation that promotes inter-subunit interactions and accumulation at repair centers, as well as by CDK1-dependent mitotic phosphorylation (PMID:25699709, PMID:27373152, PMID:26556339).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2005 High

    Discovery of RMI1/BLAP75 as a third, essential subunit of the BLM–Topo IIIα (Sgs1–Top3) complex established that the dissolvasome is a heterotrimer rather than a heterodimer, explaining why BLM alone is insufficient for genome stability.

    Evidence Co-IP, siRNA knockdown, SCE assays, and genetic epistasis in human cells and yeast, reported concurrently by three groups

    PMID:15775963 PMID:15889139 PMID:15899853

    Open questions at the time
    • Molecular mechanism by which RMI1 enhances complex activity was unknown
    • Whether RMI1 contacts DNA substrates directly during dissolution was unresolved
  2. 2006 High

    Reconstitution of dHJ dissolution with purified proteins demonstrated that RMI1 is essential for the reaction under physiological conditions, converting BLM–Topo IIIα from a minimal complex into a functional dissolvasome.

    Evidence In vitro dHJ dissolution assays with purified human BLM, Topo IIIα, and BLAP75

    PMID:16537486 PMID:16595695

    Open questions at the time
    • The specific catalytic step stimulated by RMI1 was unclear
    • Whether RMI1 also enhances helicase activity was untested
  3. 2007 High

    Mapping the functional architecture of RMI1 revealed that its N-terminal domain mediates Topo IIIα interaction (essential for dissolution) while also stimulating BLM's HJ-unwinding activity independently of topoisomerase catalysis, establishing RMI1 as both a helicase stimulator and a topoisomerase co-factor.

    Evidence Domain deletion and point mutagenesis, in vitro HJ unwinding and dissolution assays with purified proteins, yeast Top3-Rmi1 biochemistry

    PMID:17693398 PMID:17728255 PMID:18390547

    Open questions at the time
    • Structural basis of RMI1–Topo IIIα interaction was unknown
    • The precise catalytic step enhanced remained ambiguous
  4. 2010 High

    Extension of RMI1's role beyond dissolution showed it stimulates DNA end resection by Sgs1–Dna2–RPA and specifically promotes the decatenation step of Topo IIIα rather than initial branch migration, pinpointing its mechanistic contribution.

    Evidence In vitro resection and decatenation assays with purified yeast proteins; human ssDNA catenane decatenation assay

    PMID:20445207 PMID:20811461 PMID:20935631

    Open questions at the time
    • How RMI1 stimulates decatenation at the molecular level was unknown
    • Whether the resection role applies to the human BTRR complex was untested
  5. 2012 High

    Mechanistic dissection revealed that RMI1 stabilizes the open Top3–DNA covalent intermediate during strand passage, paradoxically slowing relaxation while accelerating decatenation — resolving how one cofactor shifts topoisomerase specificity from relaxation to dissolution.

    Evidence In vitro dsDNA catenation/decatenation assays with purified yeast STR and RPA

    PMID:22885009

    Open questions at the time
    • No atomic structure of the RMI1–Top3 interaction existed
    • In vivo validation of the open-gate stabilization model was lacking
  6. 2012 High

    RMI1 was shown to be required for normal replication fork progression in human cells, with epistasis placing it downstream of BLM, extending the complex's function from recombination intermediate processing to replication elongation.

    Evidence Single-molecule DNA fiber analysis (molecular combing), double-depletion epistasis, immunofluorescence

    PMID:22645306

    Open questions at the time
    • How RMI1 is recruited to stalled forks was unknown
    • Whether the fork role requires topoisomerase activity or only helicase stimulation was unresolved
  7. 2014 High

    The crystal structure of Topo IIIα–RMI1 revealed that RMI1's OB-fold attaches to the edge of Topo IIIα's DNA gate and inserts a 23-residue loop into the gate cavity, providing the structural basis for gate dynamics modulation and dissolution specificity.

    Evidence X-ray crystallography of human Topo IIIα–RMI1 complex with functional validation

    PMID:24509834

    Open questions at the time
    • Full BTRR complex structure was lacking
    • How loop insertion affects kinetics of individual catalytic steps was unmeasured
  8. 2015 High

    Top3–Rmi1 strand-passage activity was shown to be essential for all meiotic functions of Sgs1 and to have additional Sgs1-independent roles in resolving anaphase chromosome entanglements, expanding RMI1's function to meiotic recombination and chromosome segregation.

    Evidence Genetic epistasis with top3 catalytic-dead mutants, Southern blot detection of recombination intermediates, cytological analysis in S. cerevisiae meiosis across three concurrent studies

    PMID:25699707 PMID:25699708 PMID:25699709

    Open questions at the time
    • Whether the Sgs1-independent anaphase role is conserved in mammals was unknown
    • The substrate specificity of Top3-Rmi1 at anaphase bridges was uncharacterized
  9. 2016 High

    Smc5/6-mediated sumoylation of Sgs1, Top3, and Rmi1 was identified as a regulatory mechanism that promotes inter-subunit interactions and recruitment to DNA repair centers upon recombination intermediate accumulation.

    Evidence Co-IP, SUMO modification assays, 2D gel electrophoresis, fluorescence microscopy, genetic epistasis in S. cerevisiae

    PMID:27373152

    Open questions at the time
    • Specific SUMO sites on Rmi1 were not mapped
    • Whether mammalian SMC5/6 sumoylates BTRR subunits was untested
  10. 2022 High

    Single-molecule biophysics revealed that the TRR complex forms an ~8.5 nm gate in ssDNA that expands upon dsDNA binding, and that TRR orients BLM's multi-domain architecture to favor D-loop disruption, providing the physical basis for how RMI1 channels recombination toward non-crossover outcomes.

    Evidence Optical tweezers, single-molecule FRET, and in vitro D-loop disruption assays with purified human proteins

    PMID:35102151 PMID:35115525

    Open questions at the time
    • How TRR gate dynamics relate to in vivo dissolution rates was unknown
    • Structural basis for TRR-mediated BLM reorientation awaited cryo-EM
  11. 2025 High

    RNF8-mediated K63-polyubiquitylation of RMI1 at Lys428/453/566 was identified as the recruitment mechanism bringing the BTRR complex to stalled replication forks, explaining how fork-specific signaling activates the dissolvasome.

    Evidence Co-IP, ubiquitination assays, site-directed mutagenesis of lysines, immunofluorescence foci, replication fork recovery assay in human cells

    PMID:41784835

    Open questions at the time
    • Whether RNF8-mediated ubiquitylation is also required for RMI1's recombination functions is unknown
    • The deubiquitylase counteracting RNF8 on RMI1 has not been identified
  12. 2025 High

    Single-molecule kinetic analysis resolved that RMI1 enhances all three phases of TopIIIα's catalytic cycle — ssDNA trapping (the rate-limiting step), stabilization of the open cleaved complex, and substrate discrimination — quantifying how RMI1 tunes each step for hemicatenane dissolution.

    Evidence Single-molecule magnetic tweezers with mechanistic kinetic analysis of individual catalytic steps

    PMID:40266687

    Open questions at the time
    • Whether RMI2 further modulates these kinetic parameters was not addressed
    • How post-translational modifications alter catalytic cycle kinetics is unexplored

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key open questions include the full cryo-EM structure of the complete BTRR dissolvasome on substrate DNA, the identity of the deubiquitylase that counteracts RNF8 on RMI1, whether Smc5/6-mediated sumoylation of the BTRR complex occurs in mammals, and how CDK1/MPS1/PLK1-mediated phosphorylation of BTRR subunits modulates dissolvasome activity at centromeres during mitosis.
  • No full atomic structure of BTRR on substrate exists
  • Mammalian regulation by sumoylation is not established
  • Mitotic phosphorylation regulation data are currently limited to a preprint

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4 GO:0098772 molecular function regulator activity 2 GO:0003677 DNA binding 1
Localization
GO:0005634 nucleus 4 GO:0005694 chromosome 2
Pathway
R-HSA-73894 DNA Repair 6 R-HSA-1640170 Cell Cycle 4 R-HSA-69306 DNA Replication 3
Partners
BLMDNA2RAD51RMI2RNF8RPA1SMC5TOP3A
Complex memberships
BTRR dissolvasome (BLM-TopIIIα-RMI1-RMI2)STR complex (Sgs1-Top3-Rmi1)

Evidence

Reading pass · 36 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2005 RMI1 (BLAP75) physically interacts with both BLM and Topo IIIα and is an integral component of BLM complexes required for their stability in vivo; depletion of BLAP75 impairs recruitment of BLM to subnuclear foci after DNA damage, causes deficient phosphorylation of BLM during mitosis, and increases sister chromatid exchange frequency. Co-immunoprecipitation, siRNA knockdown, immunofluorescence colocalization, SCE assay The EMBO journal High 15775963
2005 Yeast Rmi1 physically interacts with Sgs1 and Top3 as a third member of the Sgs1-Top3 complex; loss of Rmi1 phenocopies sgs1 and top3 deletion (slow growth, hyperrecombination, DNA damage sensitivity); Rmi1 is a structure-specific DNA-binding protein with preference for cruciform structures, suggesting it targets the complex to recombination substrates. Co-immunoprecipitation, two-hybrid assay, genetic epistasis, in vitro DNA binding assay Molecular and cellular biology High 15899853
2005 RMI1/NCE4 encodes a third subunit of the yeast Sgs1-Top3 complex; rmi1Δ cells activate the Rad53 checkpoint and show gross chromosomal rearrangements, increased recombination, and failure to fully activate Rad53 after DNA damage. Genetic interaction network analysis, co-immunoprecipitation, checkpoint activation assay, GCR assay The EMBO journal High 15889139
2006 BLAP75/RMI1 promotes dissolution of double Holliday junctions (dHJs) catalyzed by hTOPO IIIα in a BLM-dependent manner by recruiting hTOPO IIIα to dHJs; this activity is specific to hTOPO IIIα and not seen with E. coli Top1 or Top3. In vitro dHJ dissolution assay with purified proteins, protein-DNA binding assay Proceedings of the National Academy of Sciences of the United States of America High 16537486
2006 BLAP75 (RMI1) associates independently with both Topo IIIα and BLM; under physiological conditions, dHJ dissolution by BLM-Topo IIIα becomes completely dependent on BLAP75, establishing BLM-Topo IIIα-BLAP75 as a minimal dissolvasome complex. In vitro dHJ dissolution assay with purified human proteins, pulldown, protein interaction mapping The Journal of biological chemistry High 16595695
2007 Rmi1 forms a stable Top3-Rmi1 complex that stimulates Top3 superhelical relaxation activity and ssDNA binding by Top3; Rmi1 and Top3 cooperate to bind the Sgs1 N-terminus and promote its interaction with ssDNA. In vitro supercoil relaxation assay, ssDNA binding assay, protein complex isolation from yeast overexpression The Journal of biological chemistry High 17693398
2007 Shu proteins (Csm2, Psy3, Shu1, Shu2) promote formation of Rad51/Rad54-dependent recombination intermediates (X-molecules) during S phase that are subsequently processed by the Sgs1-Rmi1-Top3 complex, placing the Shu proteins genetically upstream of Sgs1-Rmi1-Top3. Genetic epistasis, two-dimensional gel electrophoresis to detect X-molecules, fluorescence microscopy Molecular biology of the cell Medium 17671161
2007 The N-terminal third of BLAP75/RMI1 mediates complex formation with BLM and Topo IIIα and is sufficient to promote dHJ dissolution; the C-terminal DNA-binding domain of BLAP75 is dispensable for HJ processing in vitro; the K166A point mutant of BLAP75, which is defective in Topo IIIα interaction, abolishes dHJ dissolution. Domain deletion analysis, point mutagenesis, in vitro dHJ dissolution assay, pulldown The Journal of biological chemistry High 18390547
2007 Association of BLM with Topo IIIα and BLAP75 greatly enhances BLM's Holliday junction unwinding activity; this enhancement requires both Topo IIIα and BLAP75 and is specific to BLM (not WRN or RecQ), and the topoisomerase activity of Topo IIIα is dispensable for this enhancement. In vitro Holliday junction unwinding assay with purified proteins, ATPase mutant analysis The Journal of biological chemistry High 17728255
2007 Rmi1 deletion in yeast causes defective sister chromatid cohesion; loss of Rad51 suppresses the cohesion defect of rmi1 cells, and deletion of SGS1 suppresses benomyl sensitivity, indicating Rad51 and Sgs1-Top3-Rmi1 define a pathway for cohesion establishment. Genetic epistasis, sister chromatid cohesion assay, microtubule sensitivity assay EMBO reports Medium 17571075
2010 Yeast Top3-Rmi1 heterodimer stimulates DNA end resection by the Dna2-Sgs1-RPA complex in vitro by forming a complex with Sgs1, which unexpectedly stimulates DNA unwinding; Top3-Rmi1 is suggested to be important for recruitment of the Sgs1-Dna2 complex to DSBs. In vitro DNA resection assay with purified proteins, protein complex formation analysis Nature High 20811461
2010 Yeast Sgs1 and Top3 are sufficient to dissolve dHJs into exclusively non-crossover products; Rmi1 stimulates dHJ dissolution at low Sgs1-Top3 concentrations by stimulating DNA decatenation (removing the last DNA linkages) rather than affecting initial Holliday junction migration rate. In vitro dHJ dissolution assay with purified proteins at varied concentrations, decatenation assay Nature structural & molecular biology High 20935631
2010 Human Topo IIIα is a single-stranded DNA decatenase that is specifically stimulated by the BLM-RMI1 pair; RMI1 physically interacts with Topo IIIα and this interaction is required for RMI1's stimulatory effect on decatenase activity. In vitro ssDNA decatenation assay using synthetic single-stranded catenanes, protein interaction assay The Journal of biological chemistry High 20445207
2012 Sgs1, Top3, Rmi1, and RPA coordinate dsDNA decatenation through sequential single-strand passage; Rmi1 stabilizes the 'open' Top3-DNA covalent complex formed as a transient strand-passage intermediate, thereby paradoxically slowing DNA relaxation but stimulating decatenation; Sgs1 has both a catalytic and an unexpected structural role in DNA strand passage. In vitro dsDNA catenation/decatenation assay with purified proteins, mechanistic dissection Molecular cell High 22885009
2012 RMI1 is required for normal replication fork progression in human cells; the fork progression defect in RMI1-depleted cells is alleviated by BLM depletion, placing RMI1 downstream of BLM in promoting replication elongation; RMI1 localizes with BLM and TopoIIIα to subnuclear foci upon replication stress and this localization requires intact BLM-TopoIIIα-RMI1 interaction. Molecular combing (single-molecule DNA fiber analysis), siRNA knockdown, immunofluorescence, epistasis by double depletion Molecular and cellular biology High 22645306
2012 RPA inhibits Topo IIIα decatenase activity; complex formation of Topo IIIα with BLM and RMI1 ablates this RPA-mediated inhibition, suggesting BLM and RMI1 enhance Topo IIIα activity to outcompete RPA for ssDNA binding. In vitro ssDNA decatenation assay with RPA titration, protein complex formation PloS one Medium 22911760
2013 RPA physically interacts with RMI1 and stimulates BTR complex-mediated dHJ dissolution by sequestering ssDNA intermediates; RMI1 mutants defective in RPA interaction are impaired in dHJ dissolution, defining the RPA-interaction domain of RMI1 as functionally critical. In vitro dHJ dissolution assay, Co-IP, domain mutagenesis The Journal of biological chemistry High 23543748
2013 A transient α-helix in the disordered N-terminus of Sgs1 (residues 25–38) mediates binding of Top3 and Rmi1 to Sgs1; proline mutations disrupting this helix impair Top3/Rmi1 binding and cause hypersensitivity to DNA damage and increased genome rearrangements. NMR spectroscopy, in vitro binding assay, proline scanning mutagenesis, genome instability assays Nucleic acids research High 24038467
2014 Crystal structure of human TopIIIα complexed with the first OB-fold of RMI1 reveals that RMI1 attaches to the edge of the DNA-passing gate in TopIIIα and projects a 23-residue loop into the gate, thereby influencing gate dynamics to favor dissolution over relaxation activity. X-ray crystallography, functional assays validating structural model Nature structural & molecular biology High 24509834
2014 The Topo IIIα-RMI1-RMI2 complex stimulates BLM-mediated DNA unwinding and is required for processivity of DNA end resection; Topo IIIα localizes to DSB ends and contributes to 5′-to-3′ polarity of resection alongside RPA. In vitro DNA resection assay with purified human proteins, processivity measurements Nucleic acids research High 25200081
2015 Yeast Top3 disrupts Rad51-mediated D loops by a catalytic topoisomerase mechanism requiring Top3's strand-passage activity; the human TopoIIIα-RMI1-RMI2 complex is also capable of dissolving D loops, extending this mechanism to humans. In vitro D-loop dissolution assay with purified yeast and human proteins, catalytic mutant analysis Molecular cell High 25699708
2015 Top3-Rmi1 strand-passage activity is required for all known meiotic functions of Sgs1 in S. cerevisiae, including channeling joint molecules into crossover and non-crossover pathways; in addition, Top3-Rmi1 has a distinct, Sgs1-independent late role in resolving chromosome entanglements at anaphase. Genetic epistasis with top3 catalytic mutants, meiotic recombination analysis, cytological analysis Molecular cell High 25699709
2015 Top3-Rmi1 acts throughout meiotic recombination as an early recombination intermediate chaperone (Sgs1-dependent) and has essential Sgs1-independent functions ensuring complete intermediate resolution and chromosome segregation in S. cerevisiae meiosis. Genetic epistasis, Southern blot detection of recombination intermediates, cytological analysis Molecular cell High 25699707
2015 RMI1 protein level significantly increases in M phase; RMI1 is phosphorylated during mitosis primarily at Serine 284 and Serine 292, partially in a CDK1-dependent manner, without interfering with BTR complex formation. Cell synchronization, western blotting, mass spectrometry, CDK1 inhibitor (roscovitine) treatment, co-immunoprecipitation International journal of molecular sciences Medium 26556339
2016 Sgs1 binds poly-SUMO chains and associates with the Smc5/6 SUMO E3 complex; Smc5/6-mediated sumoylation of Sgs1, Top3, and Rmi1 upon generation of recombination structures promotes STR inter-subunit interactions and accumulation at DNA repair centers, facilitating removal of recombination intermediates. Co-immunoprecipitation, SUMO modification assays, two-dimensional gel electrophoresis, fluorescence microscopy, genetic epistasis Cell reports High 27373152
2019 Upon DNA damage, human RMI1 forms nuclear foci at damaged regions, interacts with RAD51, and facilitates recruitment of RAD51 to initiate homologous recombination; RMI1 depletion increases sensitivity to camptothecin and elevates DNA double-strand breaks. Immunofluorescence foci assay, co-immunoprecipitation, siRNA knockdown, comet assay FASEB journal Medium 30676768
2021 Smc5/6 co-localizes with the Sgs1-Top3-Rmi1 (STR) complex at natural pausing sites (NPSs) and facilitates Top3 retention there; depletion of individual STR subunits or Smc5/6 causes similar accumulation of joint molecules (reversed forks, dHJs, hemicatenanes), indicating Smc5/6 regulates Sgs1 and Top3 DNA processing activities at replication termination sites. ChIP-seq, two-dimensional gel electrophoresis, genetic suppression analysis, intra-allelic suppressor isolation Nature communications High 33833229
2022 The human TopoIIIα-RMI1-RMI2 (TRR) complex forms an open gate in ssDNA of ~8.5 nm; dsDNA binding to the open TRR gate increases gate size by ~16%, and BLM alters the mechanical flexibility of the gate, suggesting TRR-mediated transfer of dsDNA through strand passage in vivo. Optical tweezers, single-molecule fluorescence microscopy Nature communications High 35102151
2022 The TopoIIIα-RMI1-RMI2 (TRR) complex orients BLM helicase for efficient D-loop disruption; TRR shifts the balance of BLM's multi-domain architecture markedly toward D-loop disruption, providing a mechanism for HR pathway regulation. Single-molecule FRET, optical tweezers, D-loop disruption assay with purified proteins Nature communications High 35115525
2022 TOP3A-RMI1/2 aids BLM in initiating DNA unwinding during resection and, together with MRN, stimulates DNA2-mediated resection; MRN promotes association of the BTRR complex with DNA and synchronizes BLM and DNA2 translocation to prevent BLM pausing during resection. Single-molecule imaging, in vitro resection assay with purified human proteins The Journal of biological chemistry High 36529288
2023 In Arabidopsis, the DNA damage regulator KNO1 facilitates K63-linked ubiquitination of RMI1, triggering RMI1 autophagic degradation and increasing homologous recombination; this places RMI1 as a regulated substrate of selective autophagy controlling Bloom complex activity. Genetic analysis, ubiquitination assay, autophagy flux assay, HR reporter assay The EMBO journal Medium 36970874
2025 RMI1 is recruited to stalled replication forks via K63-linked polyubiquitylation at Lys428, Lys453, and Lys566 by the E3 ubiquitin ligase RNF8; this modification is required for proper localization of RMI1 and the entire BTR complex to stalled forks and for replication fork recovery. Co-immunoprecipitation, ubiquitination assay, site-directed mutagenesis, immunofluorescence foci analysis, replication fork recovery assay Cellular and molecular life sciences High 41784835
2025 Rmi1 helps human TopIIIα trap single-stranded DNA at the binding step (which is rate-limiting), enhances stabilization of the open cleaved complex, and improves substrate discrimination, thereby greatly increasing the efficiency of TopIIIα's catalytic cycle for hemicatenane dissolution. Single-molecule magnetic tweezers, mechanistic kinetic analysis Nucleic acids research High 40266687
2025 RAD54L2 physically interacts with BLM and suppresses sister chromatid exchanges; RAD54L2 promotes BLM recruitment to chromatin and requires an intact ATPase domain to promote non-crossover recombination, placing RAD54L2 as a regulator of the BTRR complex. Proximity labeling (BioID), co-immunoprecipitation, SCE assay, chromatin fractionation EMBO reports Medium 39870965
2026 The human TRR (TopoIIIα-RMI1-RMI2) complex relaxes highly negatively supercoiled DNA processively; TRR remains bound to DNA for long periods after torsional stress is released, consistent with a role in resolving ultrafine anaphase bridges via PICH-generated negative supercoils. Single-molecule optical tweezers combined with fluorescence imaging, real-time supercoiling density measurement Proceedings of the National Academy of Sciences of the United States of America High 41576078
2024 During early mitosis, CDK1 destabilizes the BTRR (BLM/TOP3A/RMI1/RMI2) complex and suppresses its association with PICH at centromeric chromatin; specific phosphorylation clusters on BLM targeted by the MPS1-PLK1 axis prevent BLM hyper-activation at centromeres; inactivating BLM-TOP3A interaction compromises UFB-resolution functions and can cause centromere destruction. Phospho-proteomic analysis, site-directed mutagenesis, co-immunoprecipitation, live-cell imaging, kinase inhibitor treatment bioRxivpreprint Medium

Source papers

Stage 0 corpus · 65 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2010 DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2. Nature 381 20811461
2006 BLAP75/RMI1 promotes the BLM-dependent dissolution of homologous recombination intermediates. Proceedings of the National Academy of Sciences of the United States of America 230 16537486
2006 A double Holliday junction dissolvasome comprising BLM, topoisomerase IIIalpha, and BLAP75. The Journal of biological chemistry 202 16595695
2010 Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3. Nature structural & molecular biology 161 20935631
2005 BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity. The EMBO journal 160 15775963
2005 RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex. The EMBO journal 136 15889139
2005 Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Molecular and cellular biology 134 15899853
2015 Top3-Rmi1 dissolve Rad51-mediated D loops by a topoisomerase-based mechanism. Molecular cell 103 25699708
2007 The RecQ helicase-topoisomerase III-Rmi1 complex: a DNA structure-specific 'dissolvasome'? Trends in biochemical sciences 101 17980605
2015 Pervasive and essential roles of the Top3-Rmi1 decatenase orchestrate recombination and facilitate chromosome segregation in meiosis. Molecular cell 89 25699709
2015 Top3-Rmi1 DNA single-strand decatenase is integral to the formation and resolution of meiotic recombination intermediates. Molecular cell 88 25699707
2012 Decatenation of DNA by the S. cerevisiae Sgs1-Top3-Rmi1 and RPA complex: a mechanism for disentangling chromosomes. Molecular cell 84 22885009
2007 Shu proteins promote the formation of homologous recombination intermediates that are processed by Sgs1-Rmi1-Top3. Molecular biology of the cell 84 17671161
2008 Topoisomerase 3alpha and RMI1 suppress somatic crossovers and are essential for resolution of meiotic recombination intermediates in Arabidopsis thaliana. PLoS genetics 82 19096507
2007 Holliday junction processing activity of the BLM-Topo IIIalpha-BLAP75 complex. The Journal of biological chemistry 81 17728255
2014 Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase IIIα and RMI1. Nature structural & molecular biology 73 24509834
2016 Smc5/6 Mediated Sumoylation of the Sgs1-Top3-Rmi1 Complex Promotes Removal of Recombination Intermediates. Cell reports 70 27373152
2014 Multifaceted role of the Topo IIIα-RMI1-RMI2 complex and DNA2 in the BLM-dependent pathway of DNA break end resection. Nucleic acids research 65 25200081
2008 Functional role of BLAP75 in BLM-topoisomerase IIIalpha-dependent holliday junction processing. The Journal of biological chemistry 64 18390547
2010 Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1. The Journal of biological chemistry 63 20445207
2017 RMI1 and TOP3α limit meiotic CO formation through their C-terminal domains. Nucleic acids research 61 27965412
2007 Binding and activation of DNA topoisomerase III by the Rmi1 subunit. The Journal of biological chemistry 59 17693398
2008 The Arabidopsis BLAP75/Rmi1 homologue plays crucial roles in meiotic double-strand break repair. PLoS genetics 47 19096505
2013 Role of replication protein A in double holliday junction dissolution mediated by the BLM-Topo IIIα-RMI1-RMI2 protein complex. The Journal of biological chemistry 37 23543748
2009 Association between polymorphisms in RMI1, TOP3A, and BLM and risk of cancer, a case-control study. BMC cancer 34 19432957
2011 Identification of Trypanosoma brucei RMI1/BLAP75 homologue and its roles in antigenic variation. PloS one 32 21980422
2016 Separable Roles for a Caenorhabditis elegans RMI1 Homolog in Promoting and Antagonizing Meiotic Crossovers Ensure Faithful Chromosome Inheritance. PLoS biology 30 27011106
2009 Colorectal cancer and polymorphisms in DNA repair genes WRN, RMI1 and BLM. Carcinogenesis 30 19945966
2012 RMI1 promotes DNA replication fork progression and recovery from replication fork stress. Molecular and cellular biology 28 22645306
2013 Different functions for the domains of the Arabidopsis thaliana RMI1 protein in DNA cross-link repair, somatic and meiotic recombination. Nucleic acids research 25 23956219
2013 A transient α-helical molecular recognition element in the disordered N-terminus of the Sgs1 helicase is critical for chromosome stability and binding of Top3/Rmi1. Nucleic acids research 20 24038467
2007 Genetic variant of the human homologous recombination-associated gene RMI1 (S455N) impacts the risk of AML/MDS and malignant melanoma. Cancer letters 20 17900800
2021 Smc5/6 functions with Sgs1-Top3-Rmi1 to complete chromosome replication at natural pause sites. Nature communications 19 33833229
2022 Duplex DNA and BLM regulate gate opening by the human TopoIIIα-RMI1-RMI2 complex. Nature communications 15 35102151
2010 Adipocyte hyperplasia and RMI1 in the treatment of obesity. The FEBS journal 15 21199368
2009 RMI1 deficiency in mice protects from diet and genetic-induced obesity. The FEBS journal 15 20050919
2013 Middle region of FancM interacts with Mhf and Rmi1 in silkworms, a species lacking the Fanconi anaemia (FA) core complex. Insect molecular biology 14 24286570
2010 RMI1 attenuates tumor development and is essential for early embryonic survival. Molecular carcinogenesis 13 21229605
2023 KNO1-mediated autophagic degradation of the Bloom syndrome complex component RMI1 promotes homologous recombination. The EMBO journal 12 36970874
2022 The toposiomerase IIIalpha-RMI1-RMI2 complex orients human Bloom's syndrome helicase for efficient disruption of D-loops. Nature communications 12 35115525
2022 Phenotypic spectrum of BLM- and RMI1-related Bloom syndrome. Clinical genetics 11 35218564
2013 Genome instability and embryonic developmental defects in RMI1 deficient mice. DNA repair 11 23900276
2020 miR-362 knock-down promotes proliferation and inhibits apoptosis in porcine immature Sertoli cells by targeting the RMI1 gene. Reproduction in domestic animals = Zuchthygiene 10 31916301
2019 RMI1 contributes to DNA repair and to the tolerance to camptothecin. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 10 30676768
2017 Knockdown of RMI1 impairs DNA repair under DNA replication stress. Biochemical and biophysical research communications 10 29042194
2015 Accumulation and Phosphorylation of RecQ-Mediated Genome Instability Protein 1 (RMI1) at Serine 284 and Serine 292 during Mitosis. International journal of molecular sciences 10 26556339
2013 Monopolar spindle 1 (MPS1) protein-dependent phosphorylation of RecQ-mediated genome instability protein 2 (RMI2) at serine 112 is essential for BLM-Topo III α-RMI1-RMI2 (BTR) protein complex function upon spindle assembly checkpoint (SAC) activation during mitosis. The Journal of biological chemistry 10 24108125
2007 Rmi1, a member of the Sgs1-Top3 complex in budding yeast, contributes to sister chromatid cohesion. EMBO reports 10 17571075
2022 The MRN complex and topoisomerase IIIa-RMI1/2 synchronize DNA resection motor proteins. The Journal of biological chemistry 8 36529288
2012 Rmi1 functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1. Biochemical and biophysical research communications 8 23036200
2023 RMI1 facilitates repair of ionizing radiation-induced DNA damage and maintenance of genomic stability. Cell death discovery 6 38007566
2021 Hsa_circ_0091581 promotes glioma progression by regulating RMI1 via sponging miR-1243-5p. Journal of Cancer 6 33976734
2021 Caenorhabditis elegans RMI2 functional homolog-2 (RMIF-2) and RMI1 (RMH-1) have both overlapping and distinct meiotic functions within the BTR complex. PLoS genetics 6 34252074
2012 BLM and RMI1 alleviate RPA inhibition of TopoIIIα decatenase activity. PloS one 6 22911760
2022 The topoisomerase 3 zinc finger domain cooperates with the RMI1 scaffold to promote stable association of the BTR complex to recombination intermediates in the Caenorhabditis elegans germline. Nucleic acids research 5 35639927
2011 Glucose regulates RMI1 expression through the E2F pathways in adipose cells. Endocrine 5 21432623
2015 Structural Motifs Critical for In Vivo Function and Stability of the RecQ-Mediated Genome Instability Protein Rmi1. PloS one 4 26717309
2025 Deciphering the human TopIIIα activity modulated by Rmi1 using magnetic tweezers. Nucleic acids research 3 40266687
2020 Identification and Bioinformatic Assessment of circRNA Expression After RMI1 Knockdown and Ionizing Radiation Exposure. DNA and cell biology 3 33202158
2025 The BLM-TOP3A-RMI1-RMI2 proximity map reveals that RAD54L2 suppresses sister chromatid exchanges. EMBO reports 2 39870965
2025 Comparative Transcriptomic Analysis of Soybean Recombinant Inbred Lines Differing at the Rmi1 Locus for Resistance to Meloidogyne incognita During Early Stages of Nematode Infection. Phytopathology 1 40498525
2026 Mechanistic basis for relaxation of DNA supercoils by human topoisomerase IIIα-RMI1-RMI2. Proceedings of the National Academy of Sciences of the United States of America 0 41576078
2026 K63-linked ubiquitylation of RMI1 by RNF8 is essential to its recruitment to stalled forks. Cellular and molecular life sciences : CMLS 0 41784835
2025 Candidate genes at the Rmi1 locus for resistance to Meloidogyne incognita in soybean. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 0 41160124
2024 RMI1 is essential for maintaining rice genome stability at high temperature. The Plant journal : for cell and molecular biology 0 39569466