Affinage

RAD51B

DNA repair protein RAD51 homolog 2 · UniProt O15315

Length
384 aa
Mass
42.2 kDa
Annotated
2026-04-28
85 papers in source corpus 19 papers cited in narrative 19 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAD51B is a RAD51 paralog that functions as a core component of the BCDX2 complex (RAD51B–RAD51C–RAD51D–XRCC2) and as a RAD51B–RAD51C heterodimer to promote homologous recombination-mediated DNA repair and replication fork protection. Within the BCDX2 complex, RAD51C–RAD51D–XRCC2 mimics three RAD51 protomers in a filament-like arrangement while RAD51B is dynamic; the coupled ATPase activities of RAD51B and RAD51C stimulate nucleation and extension of RAD51 filaments on ssDNA, overcoming RPA competition (PMID:37344587, PMID:11751636). RAD51B also preferentially binds branched DNA structures including Holliday junctions, and its loss causes impaired RAD51 focus formation, chromosomal instability, centrosome fragmentation, meiotic repair defects, and embryonic lethality in mice (PMID:12441335, PMID:10938124, PMID:16778173, PMID:10567591, PMID:35624308). Chromosomal rearrangements disrupting RAD51B are recurrent in uterine leiomyomas and RAD51B loss in mammary cells drives epigenetic silencing of ERα via enhanced PRC2 activity, linking its disruption to cancer pathogenesis (PMID:9892177, PMID:41318657).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1998 Medium

    Early overexpression studies established that RAD51B is not merely a structural homolog of RAD51 but participates actively in DNA damage responses, as its intact protein induces G1 delay and UV-induced apoptosis dependent on specific domains.

    Evidence Stable transfection with wild-type and mutant RAD51B constructs in CHO cells, FACS cell cycle analysis, UV survival assays

    PMID:9788630

    Open questions at the time
    • Overexpression system; physiological relevance of G1 delay unclear
    • Putative src phosphorylation site at residue 163 not validated by mass spectrometry
  2. 1999 High

    Two discoveries framed RAD51B's biological importance: homozygous disruption in mice caused early embryonic lethality (partially rescued by p53 loss), demonstrating an essential developmental role, while identification of RAD51B as a translocation partner of HMGIC in uterine leiomyomas linked its disruption to tumorigenesis.

    Evidence Mouse knockout with timed embryology and p53 double knockout; FISH and RT-PCR of fusion transcripts in leiomyoma samples

    PMID:10567591 PMID:9892177

    Open questions at the time
    • Mechanism of embryonic lethality not resolved at the molecular level
    • No functional assay demonstrating how RAD51B-HMGIC fusion drives leiomyoma formation
  3. 2000 High

    Knockout of RAD51B in DT40 cells revealed its specific cellular function: promoting RAD51 nucleoprotein filament assembly at DNA damage sites, as loss caused impaired RAD51 focus formation, chromosomal aberrations, and sensitivity to crosslinking agents. A separate study reported intrinsic serine/threonine kinase activity for RAD51B.

    Evidence DT40 gene knockout with targeted integration, SCE, RAD51 foci immunofluorescence, and survival assays; in vitro kinase assay with purified protein

    PMID:10623463 PMID:10938124

    Open questions at the time
    • Kinase activity reported by a single lab and not independently confirmed
    • Whether RAD51B kinase activity is physiologically relevant remains untested
  4. 2001 High

    Biochemical reconstitution answered how RAD51B promotes RAD51 filament formation: the RAD51B–RAD51C heterodimer acts as a recombination mediator with ssDNA-binding and ssDNA-stimulated ATPase activities that alleviate RPA competition for ssDNA, directly facilitating RAD51 loading.

    Evidence Purified protein reconstitution, ATPase assay, DNA strand exchange assay, RPA competition assay; co-IP from HeLa/MCF7/MCF10A confirming the heterodimer and larger complexes including RAD51D, XRCC2, XRCC3

    PMID:11744692 PMID:11751636

    Open questions at the time
    • Stoichiometry of the larger complexes not determined
    • Whether RAD51B–RAD51C alone is sufficient in vivo or requires the full BCDX2 complex was unknown
  5. 2002 High

    Detailed substrate specificity studies established that RAD51B preferentially and specifically binds Holliday junctions over other DNA substrates, suggesting a dedicated role in processing recombination intermediates beyond simple RAD51 filament nucleation.

    Evidence Purified RAD51B with competitive DNA-binding assays using multiple synthetic substrates (Holliday junctions, half-cruciforms, ssDNA, dsDNA)

    PMID:12427746 PMID:12441335

    Open questions at the time
    • No in vivo demonstration that RAD51B processes Holliday junctions during recombination
    • Relationship between Holliday junction binding and the mediator function unclear
  6. 2004 High

    Reconstitution of the full BCDX2 complex showed it preferentially binds branched DNA structures (Y-shaped DNA, Holliday junctions) and catalyzes strand annealing, extending the substrate preference observed for RAD51B alone to the physiological complex.

    Evidence Competitive DNA-binding assays with seven substrate types and strand-annealing assay using purified BCDX2 complex

    PMID:15141025

    Open questions at the time
    • Whether strand annealing activity contributes to second-end capture in vivo is unknown
    • Relative contributions of individual subunits to branched-DNA binding within the complex not dissected
  7. 2005 Medium

    Identification of the N-terminal KKLK motif as the nuclear localization signal for RAD51B resolved how this cytoplasmic-expressed protein reaches its site of action, and showed this import is independent of its partners RAD51C and BRCA2.

    Evidence EGFP-fusion live imaging in HeLa S3 cells with NLS mutagenesis, confirmed in RAD51C-deficient and BRCA2-deficient cell lines

    PMID:15701685

    Open questions at the time
    • Import pathway (importin identity) not determined
    • Single-lab finding; independent confirmation in other systems lacking
  8. 2006 High

    Haploinsufficiency experiments in human cells unexpectedly revealed that even 50% RAD51B reduction causes centrosome fragmentation and aneuploidy, broadening RAD51B's role beyond recombination to centrosome integrity and genome stability maintenance.

    Evidence Gene targeting in HCT116 cells and RNAi in HT1080 cells with centrosome immunofluorescence, cytogenetics, SCE, RAD51 foci, survival assays

    PMID:16778173

    Open questions at the time
    • Mechanism by which RAD51B maintains centrosome integrity is unknown
    • Whether centrosome fragmentation is a direct or indirect consequence of recombination deficiency not resolved
  9. 2014 Medium

    Studies in breast cancer cells and mouse ESCs positioned RAD51B within broader signaling networks: siRNA depletion confirmed its requirement for HR and revealed a separable cell-cycle checkpoint function, while a miR-590/Acvr2a axis was shown to regulate RAD51B expression in rapidly dividing ESCs.

    Evidence siRNA knockdown with HR reporter assay, clonogenic survival, and cell cycle analysis in breast cancer lines; miRNA overexpression with luciferase reporters in mouse ESCs

    PMID:25368520 PMID:25458897

    Open questions at the time
    • Mechanism of checkpoint function independent of HR not molecularly defined
    • miR-590/Acvr2a regulation of RAD51B shown only in mouse ESCs; human relevance untested
  10. 2022 High

    A hypomorphic RAD51B variant in mice revealed that loss of RAD51B interaction with RAD51C, RAD51, and the newly identified partner HELQ causes meiotic recombination defects, reduced crossovers, somatic chromosomal aberration repair failure, and slowed replication fork progression, unifying its roles in meiosis and mitotic DNA metabolism.

    Evidence Mouse knockin of c.92delT variant, co-IP interaction mapping, meiotic chromosome immunocytology, chromosomal aberration assay, DNA fiber assay

    PMID:35624308

    Open questions at the time
    • Functional significance of RAD51B–HELQ interaction not mechanistically dissected
    • Whether the meiotic phenotype is solely due to loss of BCDX2 function or involves additional RAD51B complexes unknown
  11. 2023 High

    Cryo-EM structure of the BCDX2 complex resolved the long-standing question of how RAD51 paralogs organize: RAD51C–RAD51D–XRCC2 forms a RAD51-filament mimic while RAD51B is dynamic, and single-molecule experiments demonstrated that the coupled ATPase activities of RAD51B and RAD51C drive RAD51 filament nucleation and extension for both replication fork protection and DSB repair.

    Evidence Cryo-EM at near-atomic resolution, AlphaFold2 modeling, structural proteomics, single-molecule ssDNA curtain assays, biochemical ATPase assays

    PMID:37344587

    Open questions at the time
    • Full-length BCDX2 on DNA not structurally resolved due to RAD51B dynamics
    • How BCDX2 is recruited to damage sites or stalled forks in vivo not determined
    • Structural basis for Holliday junction recognition by BCDX2 unknown
  12. 2025 Medium

    RAD51B loss was connected to triple-negative breast cancer development through an unexpected epigenetic mechanism: loss upregulates ATP levels, suppresses AMPK, prevents EZH2 phosphorylation, and enhances PRC2-mediated H3K27me3 silencing of ERα, which is reversible by EZH2 inhibition plus tamoxifen.

    Evidence Sleeping Beauty transposon screen in mouse mammary models, ChIP for H3K27me3, ATP measurement, AMPK/EZH2 phosphorylation assays, pharmacological rescue

    PMID:41318657

    Open questions at the time
    • Mechanism by which RAD51B loss raises cellular ATP is unknown
    • Single-lab study in mouse; human breast cancer validation pending
    • Whether this epigenetic pathway operates independently of RAD51B's recombination function not determined

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis for RAD51B dynamics within the BCDX2 complex on DNA, the mechanism by which RAD51B maintains centrosome integrity, the physiological relevance of its reported kinase activity, and the functional role of the RAD51B–HELQ interaction.
  • No structure of RAD51B engaged with DNA within the BCDX2 complex
  • Centrosome maintenance mechanism completely undefined
  • Kinase activity not independently replicated
  • RAD51B–HELQ functional relationship not characterized

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 4 GO:0140657 ATP-dependent activity 4 GO:0016787 hydrolase activity 3
Localization
GO:0005634 nucleus 3
Pathway
R-HSA-73894 DNA Repair 6 R-HSA-1640170 Cell Cycle 3 R-HSA-1643685 Disease 2 R-HSA-69306 DNA Replication 2 R-HSA-1474165 Reproduction 1
Partners
BRCA2EVLHELQRAD51RAD51CRAD51DXRCC2
Complex memberships
BCDX2 (RAD51B-RAD51C-RAD51D-XRCC2)RAD51B-RAD51C heterodimer

Evidence

Reading pass · 19 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2023 Cryo-EM structure of the BCDX2 complex (RAD51B-RAD51C-RAD51D-XRCC2) reveals that RAD51C-RAD51D-XRCC2 mimics three RAD51 protomers aligned within a nucleoprotein filament, whereas RAD51B is highly dynamic. Biochemical and single-molecule analyses showed that BCDX2 stimulates nucleation and extension of RAD51 filaments on ssDNA, dependent on the coupled ATPase activities of RAD51B and RAD51C, orchestrating RAD51 assembly for replication fork protection and double-strand break repair. Cryo-electron microscopy, AlphaFold2 modelling, structural proteomics, single-molecule analysis, biochemical ATPase assays Nature High 37344587
2001 RAD51B and RAD51C form a stable heterodimeric complex with ssDNA-binding and ssDNA-stimulated ATPase activities. The RAD51B-RAD51C complex acts as a recombination mediator by alleviating RPA competition with RAD51 for ssDNA binding, thereby facilitating assembly of the RAD51-ssDNA nucleoprotein filament. In vitro reconstitution of purified proteins, ATPase assay, DNA strand exchange assay, RPA competition assay Genes & development High 11751636
2002 Purified recombinant RAD51B and RAD51C form a highly stable heterodimer (confirmed by Ni-NTA pull-down); both proteins bind single- and double-stranded DNA and exhibit DNA-stimulated ATPase activity. RAD51C shows ATP-independent apparent DNA strand exchange/duplex destabilization activity, whereas RAD51B does not. The RAD51B-RAD51C complex interacts weakly with RAD51. Baculovirus/Sf9 purification, Ni-NTA pull-down, DNA-binding assays, ATPase assays, DNA strand exchange assay The Journal of biological chemistry High 12427746
2001 RAD51B/RAD51C heterocomplex was isolated by immunoaffinity chromatography from insect cells co-expressing the proteins and confirmed by co-immunoprecipitation from HeLa, MCF10A, and MCF7 cells. RAD51C is central to a larger complex also containing RAD51D, XRCC2, and XRCC3, but RAD51 itself is not included in these complexes. Immunoaffinity chromatography, co-immunoprecipitation from human cell lines The Journal of biological chemistry High 11744692
2002 Purified human RAD51B protein binds ssDNA and dsDNA in the presence of ATP and Mg2+ or Mn2+, hydrolyzes ATP in a DNA-dependent manner, and preferentially and specifically binds synthetic Holliday junctions over half-cruciform or dsDNA substrates, suggesting a specific role in Holliday junction processing during homologous recombinational repair. Protein purification, DNA-binding assay, ATPase assay, competitive binding with multiple DNA substrates The Journal of biological chemistry High 12441335
2004 The human BCDX2 complex (RAD51B-RAD51C-RAD51D-XRCC2) preferentially binds branched DNA structures (Y-shaped DNA and synthetic Holliday junctions) over linear ssDNA, dsDNA, tailed duplexes, or nicked duplexes. The BCDX2 complex also catalyzes strand annealing between long complementary ssDNA molecules. Competitive DNA-binding assays with seven DNA substrate types, strand-annealing assay with purified complex Nucleic acids research High 15141025
2000 Knockout of RAD51B in chicken DT40 cells causes spontaneous chromosomal aberrations, impairs homologous recombinational repair (as measured by targeted integration, SCE, and intragenic recombination), confers sensitivity to cisplatin and mitomycin C and mild sensitivity to gamma-rays, and markedly reduces damage-induced RAD51 nuclear focus formation, indicating that RAD51B promotes assembly of RAD51 nucleoprotein filaments. Gene knockout in DT40 cells, targeted integration assay, SCE assay, immunofluorescence for RAD51 foci, clonogenic survival assay Molecular and cellular biology High 10938124
1999 Homozygous disruption of muREC2/RAD51L1 in mice causes early embryonic lethality (embryos severely retarded by E5.5, resorbed by E8.5), demonstrating an essential role in cell proliferation and early embryonic development. Embryonic progression is partially rescued in a p53-null background, indicating functional interaction with p53. Homologous recombination gene knockout in ES cells, mouse embryology (timed pregnancies), blastocyst outgrowth assay, double knockout with p53-null mice Molecular and cellular biology High 10567591
2006 Haploinsufficiency of RAD51B in human HCT116 cells (generated by gene targeting) causes mild DNA damage hypersensitivity, reduced SCE, impaired RAD51 focus formation, increased chromosome aberrations, and remarkably, centrosome fragmentation and aneuploidy. RNA interference confirming ~50% RAD51B reduction in HT1080 cells also produces centrosome fragmentation, establishing RAD51B as required for centrosome integrity. Gene targeting in human HCT116 cells, RNAi in HT1080 cells, immunofluorescence for centrosomes and RAD51 foci, SCE assay, clonogenic survival, cytogenetics Cancer research High 16778173
2005 Nuclear localization of RAD51B requires an N-terminal KKLK motif (amino acids 4–7) identified as the nuclear localization signal; mutation of this motif causes cytoplasmic mislocalization of EGFP-RAD51B. Nuclear localization of RAD51B is independent of its direct binding partner RAD51C and independent of BRCA2. EGFP-fusion live imaging in HeLa S3 cells, NLS mutagenesis, immunofluorescence in Rad51C-deficient hamster irs3 cells and BRCA2-deficient CAPAN-1 cells Mutagenesis Medium 15701685
2009 Human EVL (Ena/Vasp-like) protein binds directly to both RAD51 and RAD51B proteins, stimulates RAD51-mediated homologous pairing and strand exchange, and promotes ssDNA annealing; these recombination activities are further enhanced by RAD51B. EVL knockdown impairs RAD51 assembly onto damaged DNA after ionizing radiation or mitomycin C treatment. Protein interaction assays, in vitro strand exchange and annealing assays, immunofluorescence for RAD51 foci after IR/MMC in knockdown cells The Journal of biological chemistry Medium 19329439
1998 Overexpression of wild-type RAD51B/REC2 in CHO cells induces G1 delay (cell cycle slowing) and UV-induced apoptosis. A mutation at residue 163 (putative src phosphorylation site) or N-terminal truncation abolishes these responses, implicating the intact protein including the src-site in DNA damage surveillance functions. Stable transfection, FACS cell cycle analysis, UV irradiation survival/apoptosis assays, domain mutant constructs Cancer research Medium 9788630
2000 Human RAD51B/REC2 (HsRec2/Rad51L1) exhibits serine/threonine protein kinase activity, phosphorylating kemptide, myelin basic protein, p53, cyclin E, and cdk2 but not a tyrosine-only substrate, suggesting a mechanism by which RAD51B may affect cell cycle progression. In vitro kinase assay with purified/recombinant protein and multiple substrates Experimental cell research Medium 10623463
2022 A hypomorphic RAD51B variant (c.92delT, causing translation reinitiation at Met64) abolishes or diminishes RAD51B interaction with RAD51C, RAD51, and the newly identified interactor HELQ. In mouse models, this variant causes meiotic DNA repair defects with RAD51 and HSF2BP/BMRE1 accumulation on chromosome axes, reduced crossovers, impaired repair of mitomycin-C-induced chromosomal aberrations in somatic cells, and reduced replication fork progression. Mouse knockin model, co-immunoprecipitation (interaction mapping), meiotic immunocytology, chromosomal aberration assay, DNA fiber assay for fork progression Cell death and differentiation High 35624308
1999 RAD51B (RAD51L1/hREC2) is the chromosomal translocation partner of HMGIC at 14q23-24 in uterine leiomyomas. Rearrangements within RAD51B result in allelic knockout of a uterine-specific RAD51B isoform and expression of HMGIC-RAD51B fusion transcripts, implicating RAD51B disruption in leiomyoma pathogenesis. FISH, RACE, RT-PCR of tumor samples, identification of fusion transcripts Cancer research Medium 9892177
2014 RAD51B depletion in breast cancer cell lines by siRNA leads to increased sensitivity to DNA-damaging agents (cisplatin, hydroxyurea, MMS), reduced homologous recombination efficiency, and altered cell cycle checkpoint responses; the cell cycle checkpoint influence of RAD51B is reported to be independent of its HR role. siRNA knockdown, clonogenic survival assay, HR reporter assay, cell cycle analysis by FACS Breast cancer : basic and clinical research Medium 25368520
2014 In mouse ESCs, RAD51B is a downstream effector in a miR-590/Acvr2a signaling axis; miR-590 targets Acvr2a (Activin receptor type 2a) to regulate Rad51b expression, which in turn controls SSB and DSB repair and cell cycle progression during rapid ESC proliferation. miRNA overexpression, luciferase reporter for target validation, siRNA knockdown, comet assay, flow cytometry cell cycle analysis Stem cell reports Medium 25458897
2025 RAD51 paralogs (including RAD51B) interact with BRCA2 at two distinct interaction hubs: the BRC repeats (BRC1-2) and the DNA-binding domain. The C-terminal domain (CTD) of RAD51B interacts with BRCA2, dependent on an FxxA motif on a surface-exposed, mobile region of the RAD51B CTD. Yeast 3-hybrid assay, co-immunoprecipitation, domain mapping, motif mutagenesis bioRxivpreprint Medium bio_10.1101_2024.10.10.617680
2025 Loss of RAD51B in mouse mammary cells upregulates cellular ATP levels, suppresses the AMPK pathway, causes dephosphorylation of EZH2 at Thr311, and enhances PRC2 assembly at the Esr1 promoter, resulting in H3K27me3-mediated silencing of ERα expression and TNBC phenotype development. EZH2 inhibition combined with tamoxifen reverses this and reduces TNBC progression. Sleeping Beauty transposon screen in mouse models, ChIP for H3K27me3, ATP level measurement, AMPK pathway analysis, EZH2 phosphorylation assays, pharmacological rescue Cell death & disease Medium 41318657

Source papers

Stage 0 corpus · 85 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2009 A multistage genome-wide association study in breast cancer identifies two new risk alleles at 1p11.2 and 14q24.1 (RAD51L1). Nature genetics 442 19330030
2015 Contribution of Germline Mutations in the RAD51B, RAD51C, and RAD51D Genes to Ovarian Cancer in the Population. Journal of clinical oncology : official journal of the American Society of Clinical Oncology 257 26261251
2000 The Rad51 paralog Rad51B promotes homologous recombinational repair. Molecular and cellular biology 223 10938124
2001 Mediator function of the human Rad51B-Rad51C complex in Rad51/RPA-catalyzed DNA strand exchange. Genes & development 191 11751636
1999 Disruption of muREC2/RAD51L1 in mice results in early embryonic lethality which can Be partially rescued in a p53(-/-) background. Molecular and cellular biology 132 10567591
1999 Allelic knockout of novel splice variants of human recombination repair gene RAD51B in t(12;14) uterine leiomyomas. Cancer research 114 9892177
1997 Identification of a novel human RAD51 homolog, RAD51B. Genomics 104 9441753
2002 Complex formation by the human Rad51B and Rad51C DNA repair proteins and their activities in vitro. The Journal of biological chemistry 100 12427746
1997 Isolation of human and mouse genes based on homology to REC2, a recombinational repair gene from the fungus Ustilago maydis. Proceedings of the National Academy of Sciences of the United States of America 82 9207106
2001 RAD51C interacts with RAD51B and is central to a larger protein complex in vivo exclusive of RAD51. The Journal of biological chemistry 75 11744692
2004 Preferential binding to branched DNA strands and strand-annealing activity of the human Rad51B, Rad51C, Rad51D and Xrcc2 protein complex. Nucleic acids research 72 15141025
2013 Germline mutation in the RAD51B gene confers predisposition to breast cancer. BMC cancer 64 24139550
2011 Associations of common variants at 1p11.2 and 14q24.1 (RAD51L1) with breast cancer risk and heterogeneity by tumor subtype: findings from the Breast Cancer Association Consortium. Human molecular genetics 64 21852249
2023 Structure and function of the RAD51B-RAD51C-RAD51D-XRCC2 tumour suppressor. Nature 52 37344587
2002 Holliday junction binding activity of the human Rad51B protein. The Journal of biological chemistry 50 12441335
2013 Identification of BACH2 and RAD51B as rheumatoid arthritis susceptibility loci in a meta-analysis of genome-wide data. Arthritis and rheumatism 49 24022229
2005 Arabidopsis Rad51B is important for double-strand DNA breaks repair in somatic cells. Plant molecular biology 48 15952068
2014 Three new genetic loci (R1210C in CFH, variants in COL8A1 and RAD51B) are independently related to progression to advanced macular degeneration. PloS one 47 24498017
2013 Roles of XRCC2, RAD51B and RAD51D in RAD51-independent SSA recombination. PLoS genetics 45 24278037
2006 Haploinsufficiency of RAD51B causes centrosome fragmentation and aneuploidy in human cells. Cancer research 41 16778173
2013 The Arabidopsis RAD51 paralogs RAD51B, RAD51D and XRCC2 play partially redundant roles in somatic DNA repair and gene regulation. The New phytologist 38 24102485
2013 Effects of XRCC2 and RAD51B mutations on somatic and meiotic recombination in Arabidopsis thaliana. The Plant journal : for cell and molecular biology 37 23521529
1994 The REC2 gene encodes the homologous pairing protein of Ustilago maydis. Molecular and cellular biology 37 7935431
1986 Gene amplification induces mucoid phenotype in rec-2 Pseudomonas aeruginosa exposed to kanamycin. Journal of bacteriology 36 3080408
2001 Evidence for RAD51L1/HMGIC fusion in the pathogenesis of uterine leiomyoma. Genes, chromosomes & cancer 35 11135437
1999 hREC2, a RAD51-like gene, is disrupted by t(12;14) (q15;q24.1) in a uterine leiomyoma. Cancer genetics and cytogenetics 35 10565301
2018 Target Specificity of Cas9 Nuclease via DNA Rearrangement Regulated by the REC2 Domain. Journal of the American Chemical Society 34 29874063
1985 Reexamination of phenotypic defects in rec-1 and rec-2 mutants of Haemophilus influenzae Rd. Journal of bacteriology 34 3874865
2013 A novel PLAG1-RAD51L1 gene fusion resulting from a t(8;14)(q12;q24) in a case of lipoblastoma. Cancer genetics 30 23890983
1997 Interaction between Ustilago maydis REC2 and RAD51 genes in DNA repair and mitotic recombination. Genetics 29 9071580
1994 Structure of REC2, a recombinational repair gene of Ustilago maydis, and its function in homologous recombination between plasmid and chromosomal sequences. Molecular and cellular biology 29 8065360
2016 Methylation of RAD51B, XRCC3 and other homologous recombination genes is associated with expression of immune checkpoints and an inflammatory signature in squamous cell carcinoma of the head and neck, lung and cervix. Oncotarget 28 27683114
2014 A miR-590/Acvr2a/Rad51b axis regulates DNA damage repair during mESC proliferation. Stem cell reports 27 25458897
1998 The human REC2/RAD51B gene acts as a DNA damage sensor by inducing G1 delay and hypersensitivity to ultraviolet irradiation. Cancer research 27 9788630
1994 Sequence of the rec-2 locus of Haemophilus influenzae: homologies to comE-ORF3 of Bacillus subtilis and msbA of Escherichia coli. Gene 27 8063112
2014 RAD51B plays an essential role during somatic and meiotic recombination in Physcomitrella. Nucleic acids research 26 25260587
2021 Germline RAD51B variants confer susceptibility to breast and ovarian cancers deficient in homologous recombination. NPJ breast cancer 24 34635660
1989 Cloning of the rec-2 locus of Haemophilus influenzae. Gene 24 2542131
2015 Polymorphisms of homologous recombination RAD51, RAD51B, XRCC2, and XRCC3 genes and the risk of prostate cancer. Analytical cellular pathology (Amsterdam) 23 26339569
2000 HsRec2/Rad51L1, a protein influencing cell cycle progression, has protein kinase activity. Experimental cell research 23 10623463
2016 RAD51B in Familial Breast Cancer. PloS one 20 27149063
2006 Rec2 interplay with both Brh2 and Rad51 balances recombinational repair in Ustilago maydis. Molecular and cellular biology 20 16382157
1993 The effect of rec-2 on repeat-induced point-mutation (RIP) and recombination events that excise DNA sequence duplications at the his-3 locus in Neurospora crassa. Current genetics 17 8319308
1990 Isolation of the REC2 gene controlling recombination in Ustilago maydis. Gene 17 2269439
2009 Recombination activator function of the novel RAD51- and RAD51B-binding protein, human EVL. The Journal of biological chemistry 16 19329439
2013 Genome stability of Arabidopsis atm, ku80 and rad51b mutants: somatic and transgenerational responses to stress. Plant & cell physiology 14 23574700
2014 RAD51B Activity and Cell Cycle Regulation in Response to DNA Damage in Breast Cancer Cell Lines. Breast cancer : basic and clinical research 13 25368520
2015 Germline RAD51B truncating mutation in a family with cutaneous melanoma. Familial cancer 12 25600502
1998 Analysis of the human RAD51L1 promoter region and its activation by UV light. Genomics 12 9878257
1991 Extrachromosomal recombination is deranged in the rec2 mutant of Ustilago maydis. Genetics 12 1783291
2014 In-depth analyses unveil the association and possible functional involvement of novel RAD51B polymorphisms in age-related macular degeneration. Age (Dordrecht, Netherlands) 11 24526414
2010 Gamma-radiation sensitivity and polymorphisms in RAD51L1 modulate glioma risk. Carcinogenesis 11 20610542
2001 Intragenic breakpoint within RAD51L1 in a t(6;14)(p21.3;q24) of a pulmonary chondroid hamartoma. Cytogenetics and cell genetics 11 11978964
2023 Sarcomas With RAD51B Fusions Are Associated With a Heterogeneous Phenotype. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 10 38141829
2001 Disruptions of the Ustilago maydis REC2 gene identify a protein domain important in directing recombinational repair of DNA. Molecular microbiology 10 11442839
2001 Cumulative dosage effect of a RAD51L1/HMGA2 fusion and RAD51L1 loss in a case of pseudo-Meigs' syndrome. Genes, chromosomes & cancer 10 11746973
1997 A novel link between REC2, a DNA recombinase, the retinoblastoma protein, and apoptosis. The Journal of biological chemistry 10 9235941
2016 Genetic variants within microRNA-binding site of RAD51B are associated with risk of cervical cancer in Chinese women. Cancer medicine 9 27334422
2008 Germline mutations in RAD51, RAD51AP1, RAD51B, RAD51C,RAD51D, RAD52 and RAD54L do not contribute to familial chronic lymphocytic leukemia. Leukemia & lymphoma 9 18203022
2021 Serum Levels of ARMS2, COL8A1, RAD51B, and VEGF and their Correlations in Age-related Macular Degeneration. Current neurovascular research 8 34060991
2019 RAD51B (rs8017304 and rs2588809), TRIB1 (rs6987702, rs4351379, and rs4351376), COL8A1 (rs13095226), and COL10A1 (rs1064583) Gene Variants with Predisposition to Age-Related Macular Degeneration. Disease markers 8 31191752
2017 Polymorphisms of RAD51B are associated with rheumatoid arthritis and erosion in rheumatoid arthritis patients. Scientific reports 8 28361912
2008 ERCC1 expression and RAD51B activity correlate with cell cycle response to platinum drug treatment not DNA repair. Cancer chemotherapy and pharmacology 8 18575867
2023 RAD51 and RAD51B Play Diverse Roles in the Repair of DNA Double Strand Breaks in Physcomitrium patens. Genes 7 36833232
2023 A new compact adenine base editor generated through deletion of HNH and REC2 domain of SpCas9. BMC biology 7 37434184
2023 Uterine leiomyoma with RAD51B::NUDT3 fusion: a report of 2 cases. Virchows Archiv : an international journal of pathology 7 37466765
2023 A pan-sarcoma landscape of telomeric content shows that alterations in RAD51B and GID4 are associated with higher telomeric content. NPJ genomic medicine 7 37709802
2022 A truncating variant of RAD51B associated with primary ovarian insufficiency provides insights into its meiotic and somatic functions. Cell death and differentiation 7 35624308
2020 RAD51B Levels as a Potential Predictive Biomarker for PD-1 Blockade Response in Non-Small Cell Lung Cancer. Journal of clinical medicine 7 32252414
2011 Mutation analysis of RAD51L1 (RAD51B/REC2) in multiple-case, non-BRCA1/2 breast cancer families. Breast cancer research and treatment 6 21533530
2005 Nuclear localization of Rad51B is independent of Rad51C and BRCA2. Mutagenesis 6 15701685
2022 RAD51B Harbors Germline Mutations Associated With Pancreatic Ductal Adenocarcinoma. JCO precision oncology 4 35737913
2025 CD34-positive pleomorphic uterine sarcoma with NUDT3::RAD51B fusion. Virchows Archiv : an international journal of pathology 2 40360859
2025 CRISPR-Cas12a REC2-Nuc interactions drive target-strand cleavage and constrain trans cleavage. Nucleic acids research 2 41063348
2025 Extrauterine RAD51B -Rearranged Soft Tissue Tumors : A Clinicopathologic and Molecular Genetic Study of 10 Cases With Heterogeneous Morphology, Novel Fusions, and a Distinct Methylation Profile. The American journal of surgical pathology 2 41225720
2022 A Case Study of Clinical Response to Rucaparib in a Patient with Metastatic Castration-Resistant Prostate Cancer and a RAD51B Alteration. Current oncology (Toronto, Ont.) 2 35735442
2022 The Association Between Breast Cancer and Blood-Based Methylation of CD160, ISYNA1 and RAD51B in the Chinese Population. Frontiers in genetics 2 35812748
2024 RAD51B-AS1 promotes the malignant biological behavior of ovarian cancer through upregulation of RAD51B. Journal of Zhejiang University. Science. B 1 39011678
2024 An efficient AAV vector system of Rec2 serotype for intravenous injection to study metabolism in brown adipocytes in vivo. Molecular metabolism 1 39094948
2023 Identification of a RAD51B enhancer variant for susceptibility and progression to glioma. Cancer cell international 1 37858068
2021 TBX15 rs98422, DNM3 rs1011731, RAD51B rs8017304, and rs2588809 Gene Polymorphisms and Associations With Pituitary Adenoma. In vivo (Athens, Greece) 1 33622874
2026 Epithelioid leiomyosarcoma harboring HMGA2::RAD51B fusion: expanding the spectrum of RAD51B-rearranged uterine sarcoma. Virchows Archiv : an international journal of pathology 0 41618016
2025 CRISPR-Cas12a REC2 - NUC interactions drive target-strand cleavage and constrain trans cleavage. bioRxiv : the preprint server for biology 0 40196614
2025 RAD51B-EZH2 axis as a potential therapeutic target for TNBC through cell fate conversion. Cell death & disease 0 41318657
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