Affinage

RAB14

Ras-related protein Rab-14 · UniProt P61106

Length
215 aa
Mass
23.9 kDa
Annotated
2026-06-10
82 papers in source corpus 37 papers cited in narrative 38 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAB14 is a GTP-cycling small GTPase that operates between the trans-Golgi network and the endosomal system to direct bidirectional membrane trafficking of diverse cargoes, occupying an intermediate recycling compartment positioned after Rab5/Rab4 and before Rab11 (PMID:15004230, PMID:22595670). Its activity depends on a nucleotide cycle—both GDP- and GTP-locked mutants fail to support its functions—and on geranylgeranylation by GGTase II for membrane attachment (PMID:34995578, PMID:42186738). Cargo routing through RAB14 governs surface delivery and post-endocytic sorting of ADAM10 (controlling N-cadherin and RAGE shedding) (PMID:22595670, PMID:37451218, PMID:40032916), the leaky tight-junction protein claudin-2 (which it diverts from lysosomal degradation) (PMID:24694596), GLUT4 in adipocytes and myotubes downstream of insulin/AMPK signaling via the GAP AS160/TBC1D4 (PMID:23444368, PMID:26936971, PMID:29089333), the urea transporter UT-A1 (PMID:23796783), and FGFR via the kinesin-3 motor KIF16B (PMID:21238925). RAB14 acts through class I Rab11-FIP effectors (FIP2, RCP/FIP1C, Rip11) bound in a GTP-dependent manner, and structurally engages the RCP Rab-binding domain with noncanonical stoichiometry and lower affinity than Rab11 (PMID:19702578, PMID:26032412). Beyond constitutive transport, RAB14 controls phagosomal and autophagosome maturation—the latter via direct interaction with HOPS subunits VPS39/VPS41 in overlapping roles with RAB2 (PMID:17082769, PMID:38953305)—drives lysosomal acidification by binding CAMK2D to promote V-ATPase delivery as a broad-spectrum antimicrobial restriction factor (PMID:41771894), and mediates epithelial apical polarity and lumen morphogenesis through lipid-dependent recruitment of the Par/aPKC/Cdc42 complex (PMID:18429929, PMID:27901125). Numerous pathogens co-opt RAB14, including Chlamydia and CSFV (Golgi-derived sphingolipid/ceramide delivery, sustained by Akt-mediated AS160 inactivation) (PMID:21124879, PMID:31001235, PMID:37255314) and HIV-1 Env incorporation via the RCP/FIP1C pathway (PMID:23592992).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 2004 High

    Established RAB14's basic residence and itinerary—whether it acts in the biosynthetic versus endocytic system—by mapping its localization and the effect of nucleotide-state mutants.

    Evidence Immunofluorescence, immunoEM, and dominant-active/negative mutant analysis with transferrin assays

    PMID:15004230

    Open questions at the time
    • No effectors or cargo identified
    • Did not establish how Rab14 selects between Golgi and endosomal compartments
  2. 2006 High

    Defined RAB14's first physiological role: it arrests phagosome maturation by promoting phagosome–early endosome fusion, a process exploited by intracellular mycobacteria.

    Evidence Live-cell imaging, siRNA, dominant mutants, and phagosomal fusion assays in M. tuberculosis infection

    PMID:16962593 PMID:17082769

    Open questions at the time
    • Effectors mediating fusion not identified
    • Link to AP-1 microdomain partner only Medium-confidence
  3. 2008 Medium

    Extended RAB14 into polarized epithelial and secretory trafficking, showing it directs cargo from the TGN to the apical domain and supports lamellar-body surfactant secretion.

    Evidence Yeast two-hybrid, GST pulldown, dominant-negative imaging in epithelia; Co-IP/knockdown with Annexin A2 in alveolar cells

    PMID:18332131 PMID:18429929

    Open questions at the time
    • Apical cargo receptor mechanism undefined
    • Annexin A2 interaction not placed in a defined pathway step
  4. 2009 Medium

    Identified the first RAB14 effectors—class I Rab11-FIPs—establishing how RAB14 connects to the recycling machinery and to cytokinesis.

    Evidence GTP-dependent Co-IP and confocal co-localization of FIP2/RCP/Rip11 with Rab14

    PMID:19702578

    Open questions at the time
    • Functional consequence of each FIP interaction not dissected
    • Shared use of FIPs with Rab11 not resolved
  5. 2011 High

    Linked RAB14 to a motor and a developmental program, showing KIF16B transports GTP-Rab14/FGFR vesicles in a Golgi-to-surface biosynthetic route essential for embryogenesis.

    Evidence Kif16b knockout mouse, Co-IP, dominant-negative Rab14 rescue, FGF signaling and basement membrane analysis

    PMID:21238925

    Open questions at the time
    • Rab14 GEF/GAP regulating this route not identified at the time
    • Did not address other KIF16B cargoes
  6. 2012 High

    Positioned RAB14 precisely within the recycling cascade and identified its GEF FAM116A, defining ADAM10 trafficking and N-cadherin shedding as a downstream output controlling cell migration.

    Evidence siRNA, transferrin recycling kinetics, surface ADAM10 and N-cadherin shedding/migration assays; GEF identification by yeast two-hybrid

    PMID:22595670

    Open questions at the time
    • Structural basis of FAM116A activation not defined
    • ADAM10 sorting determinant on Rab14 vesicles unknown
  7. 2013 High

    Generalized RAB14 as a cargo-sorting hub, showing it routes GLUT4 through early endosomes, recycles UT-A1, and—via RCP/FIP1C—delivers HIV-1 Env to the assembly site.

    Evidence shRNA/mutant GLUT4 surface assays in adipocytes; Xenopus oocyte UT-A1 transport; FIP1C Rab14-binding mutant rescue of HIV Env incorporation

    PMID:23444368 PMID:23592992 PMID:23796783

    Open questions at the time
    • How a single Rab14 compartment discriminates among GLUT4, UT-A1, and Env unclear
    • Insulin-regulated GAP linkage to GLUT4 not yet established here
  8. 2015 High

    Provided the structural and thermodynamic basis of the RAB14–RCP interaction, revealing noncanonical 1:2 binding and lower affinity than Rab11, clarifying effector sharing.

    Evidence X-ray crystallography, ITC, yeast two-hybrid, neuritogenesis assays

    PMID:26032412

    Open questions at the time
    • In vivo significance of the 1:2 stoichiometry not resolved
    • Membrane recruitment shown to depend on Rab11 rather than Rab14
  9. 2016 High

    Defined RAB14's role in de novo epithelial polarity and lumen morphogenesis through lipid-dependent recruitment of the Par/aPKC/Cdc42 complex, and its sorting of claudin-2 away from lysosomes (with PKCι).

    Evidence siRNA, Arf6/PI5K and wortmannin rescue, Cdc42 activity and Co-IP assays; TER and 3D cyst assays with lysosomal-inhibition rescue

    PMID:24694596 PMID:25694446 PMID:26936971 PMID:27901125

    Open questions at the time
    • Direct lipid species regulated by Rab14 not defined
    • Mechanism coupling Rab14 to Cdc42 activity state unresolved
  10. 2017 High

    Demonstrated regulated, signaling-driven RAB14 trafficking in disease, with kinase phosphorylation of RCP and EphA2 routing EphA2 through a Rab14-dependent pathway to drive metastasis.

    Evidence Phospho-mutants, kinase assays, RCP/EphA2 conditional knockout mouse, repulsion and metastasis assays

    PMID:28294115

    Open questions at the time
    • How phosphorylation switches cargo from Rab11 to Rab14 route mechanistically unclear
    • Generality beyond pancreatic adenocarcinoma not tested
  11. 2019 Medium

    Identified the prenyl escort CHML as enabling continuous RAB14 membrane recycling and linked Rab14 cargo (Mucin13, CD44) to metastatic invasion.

    Evidence Co-IP, Rab14 knockdown/overexpression, cargo identification, in vivo metastasis assays in HCC

    PMID:31175290

    Open questions at the time
    • CHML escort mechanism not biochemically reconstituted
    • Single tumor context
  12. 2024 High

    Placed RAB14 in autophagosome maturation, showing it binds HOPS subunits VPS39/VPS41 and acts in overlapping fashion with RAB2 at the fusion step.

    Evidence Comprehensive rab-KO MDCK library, LC3-II quantitation, rab2/rab14 double-KO epistasis, HOPS Co-IP

    PMID:26763909 PMID:38953305

    Open questions at the time
    • Direct contribution of Rab14 versus Rab2 to HOPS tethering not separated
    • Nucleotide regulation at autophagosomes undefined
  13. 2024 High

    Revealed a RAB14 effector–machinery (SHIP164/RhoBTB3/Vps26B) that forms early-endosome buds at Golgi-EE contact sites, with Vps26B as a novel effector.

    Evidence Co-IP, siRNA, lipid-transfer-defective mutant rescue, live imaging of EE bud formation

    PMID:38565878

    Open questions at the time
    • How Rab14 activity gates SHIP164 recruitment mechanistically unresolved
    • Cargo carried by these buds not defined
  14. 2026 High

    Established a non-trafficking signaling output: GTP-RAB14 binds CAMK2D to suppress V0a1 phosphorylation, promoting V-ATPase delivery and lysosomal acidification as broad antimicrobial restriction.

    Evidence Acidification screen, Rab14-CAMK2D Co-IP, V0a1 kinase assay, COPII binding, pathogen clearance assays

    PMID:41771894

    Open questions at the time
    • How active Rab14 inhibits CAMK2D catalysis structurally undefined
    • Relationship between this role and its endosomal trafficking role unclear
  15. 2025 Medium

    Connected RAB14 to organelle quality control, showing TGN-derived Rab14 vesicles support Parkin-dependent mitophagy through mitochondria contact sites.

    Evidence siRNA/overexpression, mito-Keima assay, Parkin/TBK1/PI3K dependency, PI(4)KIIIβ inhibition, 3D contact-site imaging

    PMID:40737294

    Open questions at the time
    • Molecular tether at Rab14-mitochondria contacts unidentified
    • Effector linking Rab14 to Parkin pathway unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how a single Rab14 compartment achieves cargo-selective sorting across its many itineraries, and what GEF/GAP/effector combinations specify each route.
  • No unified model of cargo discrimination
  • Effector repertoire for many cargoes (UT-A1, claudin-2, mitophagy) incompletely defined
  • In vivo loss-of-function phenotypes outside development largely uncharacterized

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003924 GTPase activity 3 GO:0098772 molecular function regulator activity 3
Localization
GO:0005768 endosome 4 GO:0005794 Golgi apparatus 4 GO:0005764 lysosome 3 GO:0005783 endoplasmic reticulum 2 GO:0005886 plasma membrane 2
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-168256 Immune System 3 R-HSA-5653656 Vesicle-mediated transport 3 R-HSA-9612973 Autophagy 3
Partners
ADAM10CAMK2DCDC42FIP2KIF16BRCP/FIP1CVPS39VPS41

Evidence

Reading pass · 38 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2004 Rab14 localizes to biosynthetic compartments (rough ER, Golgi, TGN) and early endosomal vacuoles/vesicles. Constitutively active Rab14Q70L shifts distribution toward early endosome-associated vesicles, while dominant-negative S25N and N124I mutants shift distribution toward the Golgi. These manipulations partially redistribute the transferrin receptor but do not affect transferrin uptake or recycling kinetics, suggesting Rab14 functions in the biosynthetic/recycling pathway between the Golgi and endosomal compartments. Immunofluorescence, immunoelectron microscopy, overexpression of dominant-active and dominant-negative Rab14 mutants, transferrin uptake/recycling assays Molecular biology of the cell High 15004230
2006 Rab14 is recruited to phagosomes containing live Mycobacterium tuberculosis following phagocytosis. Knockdown of Rab14 by siRNA or overexpression of dominant-negative mutants (Rab14S25N, Rab14N125I) releases the phagosomal maturation block, allowing phagosomes to progress to phagolysosomes. Conversely, overexpression of wild-type or constitutively active Rab14Q70L prevents dead mycobacterial phagosomes from undergoing normal maturation. Mechanistically, Rab14 stimulates organellar fusion between phagosomes and early endosomes but not late endosomes. 4D live-cell microscopy, siRNA knockdown, overexpression of dominant-negative and constitutively active mutants, phagosomal fusion assays The EMBO journal High 17082769
2006 Rab14 is part of the early endosomal AP-1 clathrin-coated microdomain at the TGN. Overexpression of a dominant-negative GTP-binding mutant that localizes exclusively to the Golgi accelerates EGF degradation, suggesting Rab14 vesicles cycle between early endosomes and Golgi cisternae within this AP-1 microdomain. Structural modeling, quantitative confocal microscopy, density centrifugation, dominant-negative overexpression, EGF degradation assay FEBS letters Medium 16962593
2008 Rab14 interacts with apical membrane proteins and localizes to the TGN and apical endosomes in polarized epithelial cells. Expression of the GDP-locked Rab14-S25N induces TGN enlargement and vesicle accumulation around Golgi membranes, and causes mislocalization of the apical raft-associated protein VIP/MAL to the basolateral domain without disrupting basolateral targeting or recycling, indicating Rab14 specifically regulates cargo delivery from the TGN to the apical domain. Yeast two-hybrid, GST pulldown, immunofluorescence, overexpression of dominant-negative Rab14-S25N, polarity assays in epithelial cells Traffic (Copenhagen, Denmark) Medium 18429929
2008 Rab14 co-immunoprecipitates with Annexin A2 in alveolar type II cells, co-localizes with Annexin A2 and lamellar bodies, and siRNA-mediated knockdown of Rab14 decreases lung surfactant secretion. Co-immunoprecipitation, co-localization by immunofluorescence, siRNA knockdown, surfactant secretion assay The Journal of biological chemistry Medium 18332131
2009 Class I Rab11-family interacting proteins (FIP2, RCP/Rab-coupling protein, Rip11) bind Rab14 in a GTP-dependent manner via their C-terminal Rab-binding domain (RBD), making them the first identified effectors of Rab14. Rab14Q70L co-localizes with Rab11a and class I FIPs at the endosomal recycling compartment during interphase. During cytokinesis, Rab14 localizes to the cleavage furrow/midbody. Co-immunoprecipitation, co-localization by confocal microscopy, GTP-dependency binding assays Biology of the cell Medium 19702578
2010 Rab14 facilitates the delivery of sphingolipids from the Golgi to Chlamydia trachomatis-containing inclusions. Dominant-negative Rab14 mutants delayed inclusion enlargement and impaired bacterial replication; siRNA silencing of Rab14 decreased bacterial multiplication and infectivity; electron microscopy showed aberrant bacteria after dominant-negative expression. Rab14 recruitment to inclusions was dependent on bacterial protein synthesis but independent of microtubules and Golgi integrity. Overexpression of dominant-negative mutants, siRNA knockdown, immunofluorescence, electron microscopy, bacterial infectivity assays (IFU), sphingolipid delivery assays PloS one High 21124879
2011 KIF16B (kinesin-3 motor) directly associates with GTP-bound Rab14 on FGFR-containing vesicles and transports them toward the plasma membrane in a Golgi-to-endosome biosynthetic pathway. Kif16b knockout mouse embryos die at peri-implantation stage, phenocopying FGFR2 knockout. Dominant-negative Rab14-GDP overexpression recapitulates defects in FGFR transport, FGF signaling, basement membrane assembly, and epiblast development. Kif16b knockout mouse model, co-immunoprecipitation, dominant-negative Rab14-GDP overexpression, embryonic phenotype analysis, FGF signaling assays, basement membrane immunostaining Developmental cell High 21238925
2012 Rab14 and its GDP-GTP exchange factor FAM116A define an intermediate recycling compartment in the transferrin-recycling pathway, positioned after Rab5/Rab4 and before Rab11. Rab14-depleted cells accumulate ADAM10 in a transferrin-positive endocytic compartment, reduce ADAM10 cell-surface levels, decrease ADAM10-mediated shedding of N-cadherin, and consequently cannot resolve cell-cell junctions, impairing cell migration. siRNA depletion, confocal microscopy, transferrin recycling assays, cell surface ADAM10 measurements, N-cadherin shedding assays, migration assays, identification of FAM116A as GEF by yeast two-hybrid/functional assays Developmental cell High 22595670
2012 Rab14 co-localizes with IRAP and syntaxin 6 in regulated endosomal storage compartments in conventional dendritic cells (cDCs). Increased recruitment of an IRAP+/Rab14+ compartment to antigen-containing vesicles correlates with superior cross-presentation efficacy of CD8+ cDCs. Immunofluorescence co-localization, IRAP-deficient DC analysis, cross-presentation assays Journal of immunology Low 22238454
2013 Rab14 binds to the C-terminus of the UT-A1 urea transporter preferentially in the GDP-bound inactive form (identified by yeast two-hybrid). Co-injection of Rab14 in Xenopus oocytes decreases UT-A1 urea transport activity by reducing cell membrane expression of UT-A1 via clathrin-mediated endocytosis (blocked by chlorpromazine but not filipin). Rab14 co-distributes with Rab5 in non-lipid raft microdomains in kidney inner medullary cells. Yeast two-hybrid, Xenopus oocyte co-injection/transport assay, cell surface biotinylation, pharmacological inhibition (chlorpromazine/filipin), co-localization with Rab5 marker FASEB journal High 23796783
2013 Rab11a-FIP1C/RCP is required for HIV-1 envelope glycoprotein (Env) incorporation onto particles in a cytoplasmic tail-dependent manner. Rab14 is required for HIV-1 Env incorporation; FIP1C mutants unable to bind Rab14 fail to rescue Env incorporation. FIP1C is redistributed by wild-type Env CT to the plasma membrane. This defines a Rab14-FIP1C endocytic recycling pathway mediating Env targeting to the assembly microdomain. siRNA depletion, dominant-negative and rescue experiments, FIP1C Rab14-binding mutants, viral particle assembly assays, Western blotting PLoS pathogens High 23592992
2013 Rab14 controls transit of internalized GLUT4 through early endosomal compartments toward the perinuclear region in 3T3-L1 adipocytes. Constitutively active Rab14Q70L reduces basal and insulin-stimulated cell-surface GLUT4 by retaining GLUT4 in an insulin-insensitive early endosomal compartment. shRNA-mediated Rab14 depletion inhibits GLUT4 transit from early endosomes to perinuclear vesicles/tubules. Overexpressed Rab14/Rab14Q70L creates enlarged ring-like early endosomal structures (~1.3 µm) that rapidly accumulate GLUT4 and transferrin by endocytosis. Confocal microscopy, shRNA knockdown, overexpression of WT and Q70L/S25N mutants, cell-surface GLUT4 quantification Journal of cell science High 23444368
2014 Rab14 knockdown in MDCK cells increases transepithelial resistance and substantially depletes claudin-2 (a leaky claudin), without affecting claudin-1, ZO-1, or occludin. Claudin-2 loss is rescued by lysosomal inhibition, indicating Rab14 sorts claudin-2 away from the lysosomal degradation pathway. Rab14 knockdown in 3D culture causes failure to form normal single-lumen cysts. MDCK I cells lacking claudin-2 endogenously show no change in TER upon Rab14 knockdown, confirming specificity. siRNA knockdown, transepithelial resistance measurement, immunofluorescence, lysosomal inhibition rescue, 3D culture lumen morphogenesis assay Molecular biology of the cell High 24694596
2015 PKCι directly interacts with Rab14 (shown by ELISA and co-immunoprecipitation). PKCι and Rab14 co-localize in intracellular puncta and at the plasma membrane. PKCι knockdown decreases claudin-2 levels; Rab14 expression is required for normal PKCι distribution in 3D cysts. The data indicate PKCι and Rab14 interact to regulate claudin-2 trafficking out of the lysosome-directed pathway. Co-immunoprecipitation, ELISA, immunofluorescence co-localization, siRNA knockdown, 3D cyst culture Molecular biology of the cell Medium 25694446
2015 Crystal structure of Rab14 in complex with the Rab-binding domain of RCP (Rab-coupling protein/FIP1C) reveals Rab14 binds RCP with reduced affinity compared to Rab11/25 and with a noncanonical 1:2 stoichiometry (Rab14:RCP) in dilute solution. In vivo, Rab11 (not Rab14) recruits RCP onto biological membranes. Both RCP and Rab14 function in neuritogenesis. X-ray crystallography, isothermal titration calorimetry, yeast two-hybrid, co-localization, neuritogenesis functional assay The Journal of biological chemistry High 26032412
2016 Rab14 knockdown disrupts polarized lipid domains and prevents the Par/aPKC/Cdc42 polarity complex from localizing to the apical membrane during de novo polarity establishment. These effects are mediated through lipid localization, as overexpression of PtdIns(4)P5K activator Arf6, PtdIns(4)P5K alone, or PI3K inhibition (wortmannin) rescues the multi-apical phenotype. Rab14 co-immunoprecipitates and co-localizes with Cdc42; Rab14 knockdown increases Cdc42 activity. Rab14 also regulates mitotic spindle orientation and midbody position. siRNA knockdown, overexpression of Arf6/PtdIns(4)P5K, pharmacological rescue (wortmannin), co-immunoprecipitation, Cdc42 activity assay, 3D MDCK culture, confocal microscopy Scientific reports High 27901125
2016 Rab14 limits sorting of Glut4 from sorting endosomes into the specialized insulin-regulated GSV (Glut4 storage vesicle) pathway in adipocytes. Both overexpression and knockdown of Rab14 decrease Glut4 plasma membrane translocation. Kinetic analysis supports that Rab14 controls sorting at the early/sorting endosome step, consistent with AS160 (a Rab GAP) regulating both Rab14 and Rab10 sequentially to control GSV entry and exocytosis. Rab14 knockdown, kinetic trafficking assays measuring Glut4 vs transferrin receptor vs LRP1, mathematical modeling of trafficking kinetics The Biochemical journal Medium 26936971
2016 Rab14 depletion in Drosophila fat body cells causes abnormal clustering of autophagosomes and lysosomes and reduces autophagosome-lysosome fusion. The kinesin Klp98A (ortholog of KIF16B) interacts with and co-localizes with Rab14, and requires Rab14 for normal localization. Rab14 is required for Klp98A-mediated autophagosome-lysosome fusion but not for vesicle positioning, indicating fusion and localization are separable events. Genetic depletion (Drosophila), co-immunoprecipitation/co-localization of Klp98A and Rab14, autophagy flux assays, autophagosome-lysosome fusion quantification Journal of cell science Medium 26763909
2017 Phosphorylation of RCP (Rab-coupling protein) at Ser435 by LMTK3 and of EphA2 at Ser897 by Akt together promote Rab14-dependent (and Rab11-independent) trafficking of EphA2 to drive cell-cell repulsion and metastasis in vivo. Genetic disruption of RCP or EphA2 opposes cell-cell repulsion and metastasis in an autochthonous mouse model of pancreatic adenocarcinoma. Phospho-specific mutants, kinase assays, RCP/EphA2 conditional knockout mouse model, cell repulsion assays, in vivo metastasis quantification Nature communications High 28294115
2017 Electrical pulse stimulation (EPS)-induced GLUT4 translocation in C2C12 myotubes requires Rab8a, Rab13, and Rab14 (siRNA knockdown of each partially reduces GLUT4 translocation), while AICAR (AMPK-mediated) GLUT4 translocation requires Rab8a and Rab14 but not Rab13 or Rab10. siRNA knockdown of individual Rabs, HA-GLUT4 surface quantitation, EPS contraction model, AMPK/CaMKII phosphorylation assays American journal of physiology. Endocrinology and metabolism Medium 29089333
2019 CHML (choroideremia-like) promotes HCC cell metastasis by facilitating Rab14 recycling to the membrane. CHML escorts Rab14 to the membrane, enabling constant Rab14 recycling. Metastasis-promoting cargoes on Rab14-positive vesicles include Mucin13 and CD44. CHML-mediated migration, invasion, and metastasis require Rab14. Co-immunoprecipitation, Rab14 knockdown, overexpression, vesicle cargo identification, in vivo metastasis assays Nature communications Medium 31175290
2019 C. trachomatis activates the Akt/AS160 signaling pathway to promote Rab14-controlled sphingolipid delivery to chlamydial inclusions. C. trachomatis induces Akt phosphorylation and recruits phospho-Akt to the inclusion membrane, inactivating the Rab14-GAP AS160/TBC1D4 by phosphorylation, thereby maintaining Rab14 in the GTP-bound active state. Akt inhibition prevents AS160 phosphorylation, reduces Rab14 recruitment to inclusions, impairs sphingolipid acquisition, and decreases bacterial multiplication. Akt inhibitor (iAkt), siRNA knockdown of AS160, phospho-Western blotting, Rab14 recruitment quantification, sphingolipid trafficking assay, bacterial infectivity (IFU), electron microscopy Frontiers in microbiology High 31001235
2020 Rab14 functions in the trafficking of Ebola virus matrix protein VP40. Proximity proteomics (BioID) identified Rab14 as a VP40-proximal protein at late stages of VP40 expression. VP40 and Rab14 substantially co-localize in HeLa cells. Overexpression of dominant-negative Rab14(S25N) diminishes VP40 plasma membrane localization. Secreted VP40 can be endocytosed into Rab14-positive compartments. BioID proximity proteomics, co-localization imaging, dominant-negative Rab14(S25N) overexpression, plasma membrane localization assay Biochemical and biophysical research communications Medium 32327259
2021 RAB14 is required for actin-based asymmetric division during mouse oocyte meiotic maturation. RAB14 localizes in the cytoplasm and accumulates at the cortex and spindle periphery during maturation. RAB14 depletion (siRNA) causes spindle migration defects and large polar bodies (failure of asymmetric division) without affecting spindle organization, mediated through the ROCK-cofilin signaling pathway for cytoplasmic actin assembly. RAB14 depletion also causes aberrant Golgi distribution. Rescue by exogenous Myc-Rab14 mRNA confirms specificity. Microinjection of siRNA and rescue mRNA, immunofluorescence for spindle/actin/Golgi, ROCK-cofilin pathway analysis (Western blot), polar body extrusion assay Cell proliferation Medium 34323331
2021 Rab14 defines a novel endocytic pathway for cationic substances (cell-penetrating peptides, polyamines, homeodomains) that is fully independent of Rab5 and Rab7, leading to non-acidic LAMP1-positive late endosomes. The pathway diverges from conventional clathrin-mediated endocytosis and macropinocytosis at the vesicle formation stage. siRNA knockdown of Rab14, Rab5, and Rab7, pharmacological inhibitors of clathrin-mediated endocytosis and macropinocytosis, live-cell imaging with fluorescent CPPs, LAMP1 co-localization Cell reports High 34731620
2022 Endocytosed HIV-1 envelope glycoprotein (Env) traffics to Rab14-positive compartments that possess hallmarks of late endosomes and lysosomes in infected CD4+ T-cell lines, where it can be recycled back to the plasma membrane. This defines a T-cell-specific late-endosomal/lysosomal Rab14-positive pathway for Env trafficking. Pulse-labeling with monovalent anti-Env Fab probe, CRISPR/Cas9 endogenous tagging of Rab GTPases, confocal microscopy, recycling assays Journal of virology Medium 35770989
2022 Geranylgeranylation of RAB14 (catalyzed by GGTase II) is required for phosphorylation of AKT at Ser473 and normal hepatic insulin signaling and glucose metabolism. Geranylgeranylation-deficient RAB14 inhibits mTORC2 complex assembly, disrupting AKT phosphorylation. Geranylgeranyl pyrophosphate supplementation rescues simvastatin-caused disruption of hepatic insulin signaling in vitro, and geranylgeraniol (GGOH) ameliorates statin-induced systemic glucose disorders in vivo. siRNA screen (small-scale), GGTase II inhibition/RABGGTA knockdown (AAV8 in vivo), GGPP/GGOH supplementation, AKT phosphorylation assays (Western blot), mTORC2 complex assembly assay, in vivo glucose metabolism tests Metabolism: clinical and experimental Medium 34995578
2023 CSFV NS5A recruits Rab14 to the ER, followed by Rab14-dependent ceramide transport to the Golgi apparatus where sphingomyelin is synthesized. The PI3K/AKT/AS160 signaling pathway regulates this Rab14-controlled ceramide transport; blocking this pathway reduces sphingomyelin content at the Golgi and impairs viral particle assembly. Rab14 knockdown/overexpression, co-localization and interaction assays, small-molecule PI3K/AKT/AS160 inhibitors, lipid quantification, viral assembly assays Journal of virology Medium 37255314
2023 AMPK activation promotes Rab14-dependent ADAM10 cell-surface translocation in human aortic endothelial cells. Rab14 siRNA knockdown prevents AICAR-induced ADAM10 surface translocation and RAGE ectodomain shedding, placing Rab14 downstream of AMPK in the ADAM10 trafficking pathway. AICAR treatment, AMPK inhibitor (Compound C), AMPKα1-siRNA, Rab14-siRNA, ADAM10 cell-surface ELISA, RAGE shedding assay Biochemical and biophysical research communications Medium 37451218
2020 CCN2 interacts with Rab14 through its IGFBP-like domain. Co-expression of CCN2 and Rab14 in COS7 cells redistributes Rab14 from diffuse cytosolic to dot-like vesicular structures that co-localize with CCN2. Dominant-negative Rab14DN reduces extracellular proteoglycan accumulation in chondrocytes. siRNA knockdown of Rab14 or CCN2 induces ER/Golgi stress markers (BIP, CHOP), suggesting they cooperate in proteoglycan-containing vesicle transport from the Golgi to endosomes. Yeast two-hybrid, in situ proximity ligation assay, overexpression of WT/CA/DN Rab14, proteoglycan secretion assay, siRNA knockdown, qRT-PCR for ER stress markers International journal of molecular sciences Medium 32316324
2024 RAB14 is a regulator of autophagosome maturation. In rab14-KO MDCK cells, LC3-II levels are altered, indicating autophagy defect specifically at the autophagosome maturation step. Double KO of rab2 and rab14 causes a severer autophagy defect than either single KO, demonstrating overlapping roles. RAB14 localizes to autophagosomes and interacts with the HOPS complex subunits VPS39 and VPS41 (confirmed by co-immunoprecipitation), similar to RAB2. Comprehensive rab-KO library in MDCK cells, LC3-II level quantification, basal and starvation-induced autophagy assays, co-immunoprecipitation of HOPS subunits, autophagosome localization imaging Autophagy High 38953305
2024 A complex of SHIP164, RhoBTB3 (ATPase), and Vps26B (retromer subunit) promotes formation of early endosome buds at Golgi-EE contact sites. Vps26B acts as a novel Rab14 effector; Rab14 activity regulates SHIP164 association with early endosomes. Depletion of SHIP164 causes enlarged Rab14+ EEs without buds, rescued by WT SHIP164 but not lipid-transfer-defective mutants. Suppression of RhoBTB3 or Vps26B mirrors SHIP164 depletion effects. Co-immunoprecipitation, siRNA depletion, overexpression of lipid-transfer-defective SHIP164 mutants, live imaging of EE bud formation, Golgi-EE contact site analysis Cell discovery High 38565878
2025 AKT activation (via SC79) translocates ADAM10 to the cell surface in human aortic endothelial cells through Rab14. Rab14 co-immunoprecipitates with ADAM10; after AKT activation Rab14 moves to the cell surface. siRNA-mediated Rab14 knockdown prevents AKT-induced ADAM10 surface translocation and RAGE ectodomain shedding, and abolishes AKT's ability to inhibit AGE-BSA-induced ICAM-1 expression. Co-immunoprecipitation of Rab14 with ADAM10, siRNA knockdown of Rab14 and AKT isoforms, surface ADAM10 quantification, RAGE shedding assay, ICAM-1 expression assay Scientific reports Medium 40032916
2025 Rab14 promotes Parkin-dependent mitophagy. Rab14 knockdown causes mitochondrial elongation and accumulation of mitochondrial proteins; Rab14 overexpression increases mitophagy (mito-Keima assay) in a Parkin-, TBK1-, and PI3K-dependent manner. 3D reconstruction reveals Rab14-mitochondria contact sites; PI(4)KIIIβ inhibition (TGN kinase) decreases these contacts and prevents Rab14-mediated mitophagy, suggesting TGN-derived Rab14 vesicles mediate the mitophagic process. siRNA knockdown, Rab14 overexpression, mito-Keima mitophagy assay, PI(4)KIIIβ inhibitor, Parkin/TBK1 dependency experiments, 3D reconstruction of contact sites Molecular biology of the cell Medium 40737294
2026 GTP-bound Rab14 acts as a broad-spectrum restriction factor against multiple bacteria and viruses by promoting V-ATPase delivery to lysosomes and lysosomal acidification. Mechanistically, active Rab14 binds CAMK2D and suppresses CAMK2D-mediated phosphorylation of V0a1 (the V-ATPase subunit determining lysosomal localization), thereby promoting V0a1 interaction with the COPII complex and facilitating V-ATPase trafficking from the ER to lysosomes. Screen for lysosomal acidification factors, Co-immunoprecipitation (Rab14-CAMK2D interaction), CAMK2D kinase assay (V0a1 phosphorylation), COPII complex binding assay, lysosomal acidification assays, pathogen clearance experiments Nature communications High 41771894
2026 Rab14 is required for human papillomavirus (HPV) transport to the trans-Golgi network (TGN) for infectious cell entry. Rab14 KO cells resist HPV pseudovirion (PsV) infection; wild-type Rab14 re-expression restores infection. Neither GDP-locked (S25N) nor GTP-locked (Q70L) mutants rescue infection, indicating that Rab14 GTPase cycling (not just one nucleotide state) is required. GDP-bound Rab14(S25N) preferentially localizes to the TGN while GTP-bound Rab14(Q70L) co-localizes with both early endosomes and TGN. Rab14 KO (CRISPR), wild-type and nucleotide-state mutant rescue, confocal microscopy tracking of PsV and Rab14 localization, infectivity assays FEBS letters Medium 42186738
2025 The RCP-RAB14 axis mediates surface delivery of MET receptor tyrosine kinase through tubulovesicular carriers to invadopodia in TNBC cells, with KIF16B promoting formation of vesicular tubules on RAB14-positive endosomes. HGF stimulation enhances MET recycling to invadopodia via this pathway, driving MT1-MMP delivery and matrix degradation. Degradation-defective MET mutant, KIF16B manipulation, RCP knockdown, RAB14-positive endosome imaging, invadopodium functional assays, MET surface delivery tracking bioRxivpreprint Low

Source papers

Stage 0 corpus · 82 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2011 MicroRNA-451 functions as a tumor suppressor in human non-small cell lung cancer by targeting ras-related protein 14 (RAB14). Oncogene 265 21358675
2004 Rab14 is involved in membrane trafficking between the Golgi complex and endosomes. Molecular biology of the cell 161 15004230
2006 Rab14 is critical for maintenance of Mycobacterium tuberculosis phagosome maturation arrest. The EMBO journal 137 17082769
2012 Rab14 and its exchange factor FAM116 link endocytic recycling and adherens junction stability in migrating cells. Developmental cell 111 22595670
2011 KIF16B/Rab14 molecular motor complex is critical for early embryonic development by transporting FGF receptor. Developmental cell 96 21238925
2010 Chlamydia trachomatis intercepts Golgi-derived sphingolipids through a Rab14-mediated transport required for bacterial development and replication. PloS one 88 21124879
2013 Rab11-FIP1C and Rab14 direct plasma membrane sorting and particle incorporation of the HIV-1 envelope glycoprotein complex. PLoS pathogens 86 23592992
2013 A role for Rab14 in the endocytic trafficking of GLUT4 in 3T3-L1 adipocytes. Journal of cell science 68 23444368
2009 Class I Rab11-family interacting proteins are binding targets for the Rab14 GTPase. Biology of the cell 66 19702578
2002 RabD, a Dictyostelium Rab14-related GTPase, regulates phagocytosis and homotypic phagosome and lysosome fusion. Journal of cell science 58 12186956
2012 Conventional dendritic cells require IRAP-Rab14 endosomes for efficient cross-presentation. Journal of immunology (Baltimore, Md. : 1950) 49 22238454
2014 MIR144 and MIR451 regulate human erythropoiesis via RAB14. British journal of haematology 48 25312678
2008 Rab14 regulates apical targeting in polarized epithelial cells. Traffic (Copenhagen, Denmark) 47 18429929
2006 Rab14 is part of the early endosomal clathrin-coated TGN microdomain. FEBS letters 46 16962593
2016 Coordination of autophagosome-lysosome fusion and transport by a Klp98A-Rab14 complex in Drosophila. Journal of cell science 44 26763909
2019 CHML promotes liver cancer metastasis by facilitating Rab14 recycle. Nature communications 43 31175290
2019 STAT3-induced upregulation of lncRNA DUXAP8 functions as ceRNA for miR-577 to promote the migration and invasion in colorectal cancer through the regulation of RAB14. European review for medical and pharmacological sciences 42 31364111
2017 Phosphorylation of Rab-coupling protein by LMTK3 controls Rab14-dependent EphA2 trafficking to promote cell:cell repulsion. Nature communications 41 28294115
2015 Rab14 regulates maturation of macrophage phagosomes containing the fungal pathogen Candida albicans and outcome of the host-pathogen interaction. Infection and immunity 41 25644001
2017 Rab14 Act as Oncogene and Induce Proliferation of Gastric Cancer Cells via AKT Signaling Pathway. PloS one 40 28107526
2017 Electrical pulse stimulation induces GLUT4 translocation in C2C12 myotubes that depends on Rab8A, Rab13, and Rab14. American journal of physiology. Endocrinology and metabolism 39 29089333
2019 TMPO-AS1 promotes cervical cancer progression by upregulating RAB14 via sponging miR-577. The journal of gene medicine 38 31483914
2014 MiR-451 increases radiosensitivity of nasopharyngeal carcinoma cells by targeting ras-related protein 14 (RAB14). Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 36 25201065
2020 LncRNA CASC15 is Upregulated in Osteosarcoma Plasma Exosomes and CASC15 Knockdown Inhibits Osteosarcoma Progression by Regulating miR-338-3p/RAB14 Axis. OncoTargets and therapy 32 33262606
2014 Rab14 regulation of claudin-2 trafficking modulates epithelial permeability and lumen morphogenesis. Molecular biology of the cell 32 24694596
2021 The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7. Cell reports 31 34731620
2016 Rab14 limits the sorting of Glut4 from endosomes into insulin-sensitive regulated secretory compartments in adipocytes. The Biochemical journal 31 26936971
2019 RAB14 activates MAPK signaling to promote bladder tumorigenesis. Carcinogenesis 29 30809635
2016 Rab14 Suppression Mediated by MiR-320a Inhibits Cell Proliferation, Migration and Invasion in Breast Cancer. Journal of Cancer 28 27994670
2017 miR-320a serves as a negative regulator in the progression of gastric cancer by targeting RAB14. Molecular medicine reports 26 28713899
2022 Serum microRNAs targeting ACE2 and RAB14 genes distinguish asymptomatic from critical COVID-19 patients. Molecular therapy. Nucleic acids 24 35721225
2019 KCNQ1OT1 promotes migration and inhibits apoptosis by modulating miR-185-5p/Rab14 axis in oral squamous cell carcinoma. Development, growth & differentiation 24 31755091
2016 Rab14 is overexpressed in ovarian cancers and promotes ovarian cancer proliferation through Wnt pathway. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 24 27718127
2013 Antagonistic control of lysosomal fusion by Rab14 and the Lyst-related protein LvsB. Traffic (Copenhagen, Denmark) 24 23387437
2008 Annexin A2 interactions with Rab14 in alveolar type II cells. The Journal of biological chemistry 24 18332131
2013 Drosophila Rab14 mediates phagocytosis in the immune response to Staphylococcus aureus. Cellular microbiology 22 24119134
2020 Long Noncoding RNA MALAT1 Promotes Colorectal Cancer Progression by Acting as a ceRNA of miR-508-5p to Regulate RAB14 Expression. BioMed research international 21 33344634
2021 LncRNA HCG18 Promotes Clear Cell Renal Cell Carcinoma Progression by Targeting miR-152-3p to Upregulate RAB14. Cancer management and research 20 33732021
2017 miR-148a increases the sensitivity to cisplatin by targeting Rab14 in renal cancer cells. International journal of oncology 20 28098870
2015 PKCι interacts with Rab14 and modulates epithelial barrier function through regulation of claudin-2 levels. Molecular biology of the cell 20 25694446
2015 Structure-Function Analyses of the Interactions between Rab11 and Rab14 Small GTPases with Their Shared Effector Rab Coupling Protein (RCP). The Journal of biological chemistry 19 26032412
2019 Akt/AS160 Signaling Pathway Inhibition Impairs Infection by Decreasing Rab14-Controlled Sphingolipids Delivery to Chlamydial Inclusions. Frontiers in microbiology 18 31001235
2018 Rab14 overexpression regulates gemcitabine sensitivity through regulation of Bcl-2 and mitochondrial function in pancreatic cancer. Virchows Archiv : an international journal of pathology 18 30267303
2024 Comprehensive knockout analysis of the RAB family small GTPases reveals an overlapping role of RAB2 and RAB14 in autophagosome maturation. Autophagy 16 38953305
2021 Long noncoding RNA SNHG20 regulates cell migration, invasion, and proliferation via the microRNA-19b-3p/RAB14 axis in oral squamous cell carcinoma. Bioengineered 16 34282711
2016 Rab14 specifies the apical membrane through Arf6-mediated regulation of lipid domains and Cdc42. Scientific reports 16 27901125
2023 RAB14 promotes epithelial-mesenchymal transition in bladder cancer through autophagy‑dependent AKT signaling pathway. Cell death discovery 15 37558664
2021 Long non-coding RNA LINC00173 enhances cisplatin resistance in hepatocellular carcinoma via the microRNA-641/RAB14 axis. Oncology letters 15 33777195
2021 RAB14 GTPase is essential for actin-based asymmetric division during mouse oocyte maturation. Cell proliferation 15 34323331
2023 The Small GTPase Rab14 Regulates the Trafficking of Ceramide from Endoplasmic Reticulum to Golgi Apparatus and Facilitates Classical Swine Fever Virus Assembly. Journal of virology 14 37255314
2011 Golgi-associated Rab14, a new regulator for Chlamydia trachomatis infection outcome. Communicative & integrative biology 14 22046472
2021 Long Non-Coding RNA CCAT2 Activates RAB14 and Acts as an Oncogene in Colorectal Cancer. Frontiers in oncology 13 34868956
2020 Proximity proteomics identifies novel function of Rab14 in trafficking of Ebola virus matrix protein VP40. Biochemical and biophysical research communications 13 32327259
2013 Small GTPase Rab14 down-regulates UT-A1 urea transport activity through enhanced clathrin-dependent endocytosis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 13 23796783
2022 Endocytosed HIV-1 Envelope Glycoprotein Traffics to Rab14+ Late Endosomes and Lysosomes to Regulate Surface Levels in T-Cell Lines. Journal of virology 11 35770989
2020 Rab14 Overexpression Promotes Proliferation and Invasion Through YAP Signaling in Non-Small Cell Lung Cancers. OncoTargets and therapy 10 32982313
2020 Roles of Interaction between CCN2 and Rab14 in Aggrecan Production by Chondrocytes. International journal of molecular sciences 9 32316324
2022 MiR-214-3p targets Ras-related protein 14 (RAB14) to inhibit cellular migration and invasion in esophageal Cancer cells. BMC cancer 8 36471277
2021 CircTMCO3 Promotes Gastric Cancer Progression by Regulating miR-577/RAB14 Axis. Cancer management and research 8 34377026
2022 Mevalonate pathway orchestrates insulin signaling via RAB14 geranylgeranylation-mediated phosphorylation of AKT to regulate hepatic glucose metabolism. Metabolism: clinical and experimental 7 34995578
2017 Silencing Rab14 represses the proliferation and migration of oral squamous cell carcinoma, and enhances cisplatin sensitivity. American journal of translational research 7 28979693
2021 Akt Phosphorylation Influences Persistent Chlamydial Infection and Chlamydia-Induced Golgi Fragmentation Without Involving Rab14. Frontiers in cellular and infection microbiology 6 34169005
2012 The art of "cut and run": the role of Rab14 GTPase in regulating N-cadherin shedding and cell motility. Developmental cell 6 22595666
2024 Biogenesis of Rab14-positive endosome buds at Golgi-endosome contacts by the RhoBTB3-SHIP164-Vps26B complex. Cell discovery 5 38565878
2007 Isolation, sequence identification and tissue expression distribution of three novel porcine genes--RAB14, S35A3 and ITM2A. Molecular biology reports 5 17380425
2023 MBNL1-AS1 Promotes Hypoxia-Induced Myocardial Infarction via the miR-132-3p/RAB14/CAMTA1 Axis. Oxidative medicine and cellular longevity 4 36785792
2017 MicroRNA-451 dictates the anoikis resistance of osteosarcoma by targeting Rab14. International journal of clinical and experimental pathology 4 31966443
2025 AKT activation triggers Rab14-mediated ADAM10 translocation to the cell surface in human aortic endothelial cells. Scientific reports 3 40032916
2024 Histone deacetylase inhibition disrupts the molecular signature of the glioblastoma secretome related to extracellular vesicle-associated proteins and targets RAB7a and RAB14 in vitro. Biochemical and biophysical research communications 3 39454304
2015 Rab antibody characterization: comparison of Rab14 antibodies. Methods in molecular biology (Clifton, N.J.) 3 25800840
2010 Rab14 from Bombyx mori (Lepidoptera: Bombycidae) shows ATPase activity. Biology letters 3 20071392
2025 Rab14 promotes Parkin-mediated mitophagy. Molecular biology of the cell 2 40737294
2024 Restoring cellular copper homeostasis in Alzheimer disease: a novel peptide shuttle is internalized by an ATP-dependent endocytosis pathway involving Rab5- and Rab14-endosomes. Frontiers in molecular biosciences 2 38645276
2024 Circular RNA IARS modulates the progression and ferroptosis of osteosarcoma via sponging miR-188-5p from RAB14. Neoplasma 2 38958715
2023 AMPK boosts ADAM10 shedding activity in human aortic endothelial cells by promoting Rab14-dependent ADAM10 cell surface translocation. Biochemical and biophysical research communications 2 37451218
2023 A Fluorescent Vector of Carbon Dot to Deliver Rab13 and Rab14 Plasmids for Promoting Neurite Outgrowth. ACS applied bio materials 2 37679053
2021 ILF3-AS1 promotes cell proliferation and inhibits cell apoptosis of breast cancer by binding with miR-4429 to upregulate RAB14. Human & experimental toxicology 2 33525948
2026 Rab14 restricts pathogens by promoting V-ATPase lysosomal delivery to drive lysosomal acidification. Nature communications 1 41771894
2026 The Role of LINC01569/miR-4722-5p/RAB14 Regulatory Axis in Chronic Obstructive Pulmonary Disease-Related Inflammation and Its Clinical Value Analysis. Journal of biochemical and molecular toxicology 0 41536203
2026 Rab14-Mediated Lipophagy Disrupts Lipid Homeostasis in Granulosa Cells and Impairs Female Fertility. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 0 41553083
2026 Rab14 regulates the transport of human papillomavirus to the trans-Golgi network for infectious cell entry. FEBS letters 0 42186738
2023 Imaging of Molecular Interaction Between CCN Protein and Its Binding Partners: An In Situ Proximity Ligation Assay of Interaction Between CCN2 and Rab14 in Chondrocytes. Methods in molecular biology (Clifton, N.J.) 0 36370342

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