Affinage

RAB13

Ras-related protein Rab-13 · UniProt P51153

Length
203 aa
Mass
22.8 kDa
Annotated
2026-06-10
54 papers in source corpus 32 papers cited in narrative 32 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RAB13 is a small GTPase that controls polarized membrane trafficking at the cell periphery, governing tight junction assembly, directed cell migration, and regulated exocytosis (PMID:12058051, PMID:25713415). In its GTP-bound state it engages the effector MICAL-L2/JRAB, which links RAB13 to the actin cytoskeleton and mediates endocytic recycling of the tight junction proteins occludin and claudin-1 back to the plasma membrane to build functional junctions (PMID:15528189, PMID:16525024, PMID:18779367); MICAL-L2 coordinates this with adherens junction assembly by alternately binding Rab8 at perinuclear recycling compartments and RAB13 at the cell surface (PMID:18094055), and RAB13 binding induces a conformational change in MICAL-L2 that recruits actinin and filamin isoforms to remodel actin (PMID:20008558, PMID:23100251). RAB13 also acts as a brake on tight junctions by directly binding and inhibiting the catalytic subunit of PKA, preventing PKA-dependent recruitment of VASP, ZO-1, and claudin-1 to junctions (PMID:15096524, PMID:16473634). Beyond epithelia, RAB13 drives insulin- and Ca2+-stimulated GLUT4 vesicle exocytosis downstream of the Rab-GAP AS160 in muscle cells, assembling with MICAL-L2 and alpha-actinin-4 (PMID:21041651, PMID:26538022, PMID:29089333), delivers integrin LFA-1 to the leading edge of lymphocytes downstream of the GEF DENND1C and the kinase Mst1 (PMID:25074980), and recycles active beta1-integrin and CXCR1/2 to support adhesion, migration, and tumor cell behavior (PMID:31076515, PMID:35395074). RAB13 is activated locally: the GEF DENND2B activates it at the leading edge of migrating cells (PMID:25713415), while peripheral localization and translation of RAB13 mRNA at protrusions couples nascent RAB13 to the exchange factor RABIF for full GTPase activation and polarized filopodia dynamics (PMID:32946136, PMID:32946121). Its activity is negatively regulated by RNF115-catalyzed K11-linked ubiquitination on Lys46/Lys58, which impairs GDI1 recruitment and post-Golgi trafficking (PMID:35343654).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1998 Medium

    Established that RAB13's membrane association is dynamically controlled, identifying delta-PDE rather than GDI as a factor able to extract it from membranes.

    Evidence Yeast two-hybrid screen and in vitro membrane dissociation with recombinant delta-PDE

    PMID:9712853

    Open questions at the time
    • Physiological role of delta-PDE in RAB13 cycling not established in cells
    • Relationship to canonical GDI-mediated recycling left open
  2. 2002 High

    Placed RAB13 at junctional complexes and showed its GTP cycling governs tight junction barrier function, defining a junctional role for the GTPase.

    Evidence GFP-RAB13 Q67L/T22N mutant cell lines in MDCK with TEER, freeze-fracture EM, and tracer assays

    PMID:12058051

    Open questions at the time
    • Effector mediating the junctional effect not yet identified
    • Trafficking step affected not resolved
  3. 2004 High

    Defined the cargo selectivity of RAB13 (occludin recycling) and uncovered a non-trafficking mechanism—direct PKA inhibition controlling junction protein recruitment.

    Evidence Biotinylation/recycling assays plus Co-IP and in vitro PKA activity assays with purified RAB13

    PMID:15096524 PMID:15528189 PMID:16473634

    Open questions at the time
    • How RAB13 toggles between trafficking and PKA-inhibitory functions unclear
    • Structural basis of PKA binding not defined
  4. 2006 High

    Identified MICAL-L2/JRAB as the GTP-dependent effector linking RAB13 to occludin recycling and the actin cytoskeleton, providing the molecular bridge for junction assembly.

    Evidence Yeast two-hybrid, reciprocal Co-IP, recycling and Ca2+-switch assays in epithelial cells

    PMID:16525024

    Open questions at the time
    • How MICAL-L2 couples to the recycling machinery mechanistically unresolved
  5. 2007 High

    Showed MICAL-L2 is shared by Rab8 and RAB13 in a competitive, sequential handoff coordinating tight and adherens junction assembly, and that RAB13 acts in TPA-induced scattering.

    Evidence siRNA, Co-IP, dominant mutants, Ca2+-switch and scattering assays in MDCK

    PMID:17891173 PMID:18094055

    Open questions at the time
    • Spatial/temporal switch between Rab8 and RAB13 on MICAL-L2 not directly visualized
    • Activation trigger during scattering not defined
  6. 2008 High

    Mapped RAB13's itinerary to the TGN-to-recycling-endosome route, distinguishing it from direct TGN-to-surface trafficking and clarifying which cargoes depend on it.

    Evidence shRNA knockdown and dominant mutants with cargo-selective surface arrival assays

    PMID:18779367

    Open questions at the time
    • Molecular machinery executing the TGN-RE step not identified
  7. 2010 High

    Extended RAB13 to regulated exocytosis, placing it downstream of the Rab-GAP AS160 in insulin-stimulated GLUT4 delivery in muscle cells.

    Evidence GTP-loading assays, siRNA, AS160 epistasis, and imaging in L6 muscle cells

    PMID:21041651

    Open questions at the time
    • GEF activating RAB13 downstream of AS160 not identified in this system
    • Effector for GLUT4 vesicles not yet defined here
  8. 2014 High

    Identified the lymphocyte GEF DENND1C/Mst1 pathway and demonstrated, with knockout mice, a physiological RAB13 role in LFA-1 delivery and immune cell trafficking.

    Evidence DENND1C phosphorylation and RAB13 activation assays, RAB13 knockout mice, LFA-1 trafficking and adhesion assays

    PMID:25074980

    Open questions at the time
    • Effector linking RAB13 to LFA-1 vesicles beyond myosin Va recruitment incompletely defined
  9. 2015 High

    Identified DENND2B as the leading-edge GEF and demonstrated spatially restricted RAB13 activation driving migration and cancer invasion; defined the insulin-responsive MICAL-L2/ACTN4/GLUT4 complex.

    Evidence FRET-based RAB13 biosensor, GEF assays, Co-IP, invasion and xenograft assays; super-resolution imaging of GLUT4 complex

    PMID:25713415 PMID:26538022

    Open questions at the time
    • How DENND2B is itself spatially confined to the leading edge unresolved
    • Direct effector mediating GLUT4 vesicle tethering not defined
  10. 2017 Medium

    Refined the GLUT4 exocytic program by showing RAB13 selectively serves Ca2+/PKC- and contraction-stimulated translocation, distinguishing it from other Rab family members.

    Evidence Selective siRNA of Rab family members with cargo-specific GLUT4 surface assays and EPS contraction model

    PMID:29089333 PMID:29247648

    Open questions at the time
    • Signaling node connecting Ca2+/PKC to RAB13 activation not identified
    • Single-cell-type evidence
  11. 2016 Medium

    Showed prenylation is dispensable for inactive RAB13 vesicle association, revealing protein-protein interactions rather than lipid anchoring govern GDP-state localization.

    Evidence Prenylation-deficient mutants with fractionation and live-cell vesicle imaging

    PMID:26969162

    Open questions at the time
    • Identity of the protein partners anchoring inactive RAB13 to vesicles unknown
  12. 2019 Medium

    Expanded RAB13 cargo to active beta1-integrin recycling via GGA2, linking it to focal adhesion turnover and migration.

    Evidence RNAi screen, BioID proximity labeling, integrin activity and migration assays

    PMID:31076515

    Open questions at the time
    • GGA2-RAB13 interaction by BioID without reciprocal direct-binding validation
    • Mechanism of conformer-selective integrin recycling unresolved
  13. 2020 High

    Established that localized RAB13 mRNA translation at protrusions couples nascent protein to RABIF for full GTPase activation, creating a polarized domain driving filopodia dynamics and vessel patterning in vivo.

    Evidence RNA localization perturbation, RABIF co-translational interaction, GTPase and migration assays; smFISH, CRISPR excision of a 192-nt element, zebrafish imaging

    PMID:32946121 PMID:32946136

    Open questions at the time
    • How RABIF co-translational engagement selects RAB13 over other Rabs unresolved
    • Relationship between RABIF and other GEFs (DENND2B) not integrated
  14. 2022 High

    Defined RNF115-catalyzed K11-linked ubiquitination on Lys46/58 as a negative regulator that blocks GDI1 recruitment and post-Golgi TLR trafficking, adding a post-translational control layer.

    Evidence Co-IP, ubiquitination assays, site-directed K46/58R mutagenesis, GDI1 recruitment and TLR trafficking assays

    PMID:35343654

    Open questions at the time
    • Deubiquitinase reversing the modification not identified
    • How ubiquitination is spatially restricted unknown
  15. 2022 Medium

    Linked RAB13 to cancer stem cell niche formation via surface translocation of CXCR1/2 receptors.

    Evidence siRNA, surface receptor measurement, co-culture and in vivo tumorigenesis assays in breast cancer cells

    PMID:35395074

    Open questions at the time
    • Direct effector handling CXCR1/2 vesicles not defined
    • Single-lab functional readout

Open questions

Synthesis pass · forward-looking unresolved questions
  • How RAB13 is selectively activated and ubiquitinated at distinct subcellular sites, and how a single GTPase coordinates its many cargoes (junction proteins, GLUT4, integrins, chemokine receptors) across cell types, remains unresolved.
  • No unifying model integrating multiple GEFs (DENND2B, DENND1C, RABIF) with cargo selection
  • Structural basis of effector and PKA binding undefined
  • Deubiquitinase and full ubiquitin-cycle regulation unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003924 GTPase activity 4 GO:0098772 molecular function regulator activity 1
Localization
GO:0005886 plasma membrane 5 GO:0005768 endosome 3 GO:0005794 Golgi apparatus 2 GO:0031410 cytoplasmic vesicle 2 GO:0005829 cytosol 1
Pathway
R-HSA-5653656 Vesicle-mediated transport 5 R-HSA-9609507 Protein localization 3 R-HSA-162582 Signal Transduction 2 R-HSA-168256 Immune System 2 R-HSA-1474244 Extracellular matrix organization 1
Partners
ACTN4DENND1CDENND2BGGA2GRB2MICALL2PRKACARABIF

Evidence

Reading pass · 32 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2002 Rab13 is recruited to junctional complexes from a cytosolic pool after cell-cell contact formation. Constitutively active Rab13 (Q67L) delays formation of electrically tight epithelial monolayers, disrupts the tight junction fence diffusion barrier, and delays claudin-1 localization at the cell surface, while inactive Rab13 (T22N) has no effect on TJ function or structure. Stable GFP-Rab13 mutant cell lines (Q67L and T22N) in MDCK cells; transepithelial electrical resistance; freeze-fracture EM; immunofluorescence; tracer leakage assays Molecular biology of the cell High 12058051
1998 The rod cGMP phosphodiesterase delta subunit (delta-PDE) specifically binds Rab13 and purified recombinant delta-PDE dissociates Rab13 from cellular membranes, suggesting delta-PDE (not GDI) controls the dynamic association of Rab13 with membranes. Yeast two-hybrid screen of HeLa cDNA library; purified recombinant delta-PDE membrane dissociation assay; immunofluorescence The Journal of biological chemistry Medium 9712853
2004 Rab13 dominant active mutant (Q67L) specifically inhibits postendocytic recycling of occludin back to the cell surface, but not recycling of transferrin receptor or polymeric immunoglobulin receptor; endocytosed occludin colocalizes with Rab13. Cell surface biotinylation; biochemical endocytosis and recycling assays; double immunolabeling; dominant active Rab13 expression in BHK and MTD-1A cells The Journal of biological chemistry High 15528189
2004 GTP-bound Rab13 directly binds to the alpha-catalytic subunit of PKA and inhibits PKA activity, thereby preventing PKA-dependent phosphorylation and tight junction recruitment of VASP (vasodilator-stimulated phosphoprotein). Activation of PKA abrogates the inhibitory effect of Rab13 on recruitment of VASP, ZO-1, and claudin-1 to cell-cell junctions. Co-immunoprecipitation; in vitro PKA activity assay with purified Rab13; dominant active Rab13 expression; PKA activator rescue experiments The Journal of cell biology High 15096524 16473634
2006 MICAL-L2 (renamed JRAB, junctional Rab13-binding protein) specifically binds to the GTP-bound form of Rab13 via its C-terminal coiled-coil domain, localizes to tight junctions, and mediates endocytic recycling of occludin and formation of functional tight junctions. A MICAL-L2 mutant lacking the Rab13-binding domain (MICAL-L2-N) inhibits occludin recycling and TJ formation. MICAL-L2 links Rab13 to the actin cytoskeleton and is displaced from TJs upon actin depolymerization. Yeast two-hybrid; immunoprecipitation; immunofluorescence; recycling assay; Ca2+ switch assay; transepithelial electrical resistance measurement Molecular biology of the cell High 16525024
2007 JRAB/MICAL-L2 interacts with both Rab13 and Rab8 via its C-terminal domain, with Rab8 and Rab13 competing for MICAL-L2 binding. Rab13 specifically mediates claudin-1 and occludin transport to the plasma membrane but not E-cadherin transport, while Rab8 mediates Rab13-independent E-cadherin transport. JRAB/MICAL-L2 coordinates TJ and AJ assembly by sequentially interacting with Rab8 at perinuclear recycling compartments and Rab13 at the plasma membrane. siRNA knockdown; Ca2+ switch model; co-immunoprecipitation; immunofluorescence; dominant-negative and constitutively active mutant expression Molecular biology of the cell High 18094055
2008 Rab13 regulates membrane trafficking between the trans-Golgi network (TGN) and recycling endosomes (RE). Rab13 partially colocalizes with TGN38 at the TGN and transferrin receptors in RE. Knockdown or dominant mutant expression of Rab13 disrupts TGN38/46 localization and inhibits surface arrival of proteins that transit through RE (VSVG, A-VSVG, LDLR-CT27), but not proteins using a direct TGN-to-PM route. shRNA knockdown in HBE cells; dominant-active and dominant-negative allele overexpression in MDCK cells; immunofluorescence colocalization; surface protein arrival assays The Journal of cell biology High 18779367
2009 Rab13 regulates neurite outgrowth in PC12 cells through its effector JRAB/MICAL-L2. Rab13 binding to JRAB/MICAL-L2 stimulates an intramolecular conformational change in JRAB/MICAL-L2 and promotes its interaction with actinin-4, an actin-binding protein that localizes to the cell body and neurite tips. This complex is proposed to transfer actinin-4 to neurite tips to reorganize actin for neurite extension. Dominant active Rab13 expression; co-immunoprecipitation; immunofluorescence; neurite outgrowth assays in PC12 cells Molecular and cellular biology Medium 20008558
2010 Insulin promotes GTP loading of Rab13 (and Rab8A) in rat L6 muscle cells. siRNA-mediated Rab13 knockdown blocks insulin-induced GLUT4 appearance at the muscle cell surface, rescued by Rab13 ortholog but not Rab8A. Constitutively active AS160 (Rab-GAP) suppresses surface GLUT4, reversed by Rab13 or Rab8A overexpression, placing both Rabs downstream of AS160 in GLUT4 exocytic trafficking. Insulin promotes Rab13 colocalization with GLUT4 at the cell periphery. GTP-loading assay; siRNA knockdown; surface GLUT4 measurement; constitutively active AS160 expression; immunofluorescence colocalization Proceedings of the National Academy of Sciences of the United States of America High 21041651
2011 Rab13 is required for trafficking of a protein complex (RhoA, Syx, Mupp1, angiomotin) to the leading edge of migrating endothelial cells. Rab13 associates with Grb2, targeting Syx and RhoA to Tyr1175-phosphorylated VEGFR2 at the leading edge. Rab13 knockdown in zebrafish impedes sprouting of intersegmental vessels and reduces tip cell directionality. siRNA knockdown; co-immunoprecipitation; live-cell imaging; zebrafish vessel sprouting assay; directed migration assay The Journal of biological chemistry Medium 21543326
2012 JRAB/MICAL-L2 interacts with actin-binding proteins actinin-1 and actinin-4 and filamentous actin via different domains, regulating actin cross-linking and stabilization. During epithelial junctional development, JRAB is enriched in the actin bundle at the free border; Rab13 binding induces a conformational change in JRAB required for maturation of cell-cell adhesion sites. Co-immunoprecipitation; immunofluorescence; actin bundling assays; dominant active Rab13 expression; Ca2+ switch assay The Journal of biological chemistry Medium 23100251
2014 Rab35 functions as a master Rab that recruits MICAL-L1 to Arf6-positive recycling endosomes, and MICAL-L1 in turn recruits Rab13 (along with Rab8 and Rab36) to these endosomes during NGF-induced neurite outgrowth in PC12 cells. Rab13 regulates neurite outgrowth non-redundantly downstream of Rab35 and MICAL-L1. Dominant active/negative Rab mutant expression; siRNA knockdown; immunofluorescence; neurite outgrowth assays in PC12 cells Biology open Medium 25086062
2014 In lymphocytes, chemokine stimulation activates Rab13 via DENND1C (a GEF phosphorylated by kinase Mst1). Active Rab13 associates with Mst1 to facilitate delivery of integrin LFA-1 to the leading edge, involving myosin Va recruitment along actin filaments. Rab13-deficient mice have reduced lymphocyte numbers in lymphoid tissues due to defective trafficking. Inhibiting Rab13 function reduces lymphocyte adhesion and migration on ICAM-1 and disrupts the ring-like LFA-1 arrangement at the T cell-APC contact site. DENND1C phosphorylation assays; Rab13 activation assays; Rab13 knockout mice; LFA-1 surface trafficking assays; lymphocyte adhesion and migration assays; immunofluorescence Science signaling High 25074980
2015 DENND2B is the guanine nucleotide exchange factor (GEF) for Rab13. DENND2B activates Rab13 specifically at the leading edge of migrating cells, as demonstrated by a FRET-based Rab13 biosensor. DENND2B interacts with the Rab13 effector MICAL-L2 at the cell periphery, and this interaction is required for dynamic remodeling of the leading edge. Disruption of Rab13-mediated trafficking limits invasive behavior in vitro and growth/migration of cancer cells in vivo. FRET-based Rab13 biosensor; GEF activity assay; co-immunoprecipitation; in vitro invasion assays; in vivo tumor xenograft assays The Journal of cell biology High 25713415
2015 Rab13 forms an insulin-dependent complex with MICAL-L2 and alpha-actinin-4 (ACTN4) in muscle cells. Insulin increases Rab13 binding to MICAL-L2, which in turn binds ACTN4. GLUT4 associates with this complex in response to insulin, requiring the ACTN4-binding domain in MICAL-L2. Knockdown of MICAL-L2 or expression of truncated MICAL-L2 impairs insulin-stimulated GLUT4 translocation. Pull-down assays; confocal fluorescence microscopy; structured illumination microscopy (SIM); TIRF microscopy; siRNA knockdown; co-immunoprecipitation Molecular biology of the cell High 26538022
2016 C-terminal prenylation is not required for Rab13 to associate with and traffic on vesicles in its inactive GDP-bound state; inactive Rab13 associates with vesicles via protein-protein interactions. Only upon activation does Rab13 associate with the plasma membrane, presumably via C-terminal prenyl group membrane insertion. Prenylation-deficient Rab13 mutant expression; subcellular fractionation; live-cell vesicle trafficking imaging The Journal of biological chemistry Medium 26969162
2017 Rab13 is required for ionomycin-stimulated GLUT4 exocytosis in L6 myoblasts. siRab13 (but not siRab8a, siRab10, or siRab14) selectively inhibited GLUT4 exocytosis promoted by elevated cytosolic Ca2+, placing Rab13 specifically downstream of Ca2+/PKC signaling in this pathway. siRNA knockdown; surface GLUT4myc exocytosis assay; ionomycin-stimulated Ca2+ elevation; phosphorylation assays for AS160 and TBC1D1 Biochemical and biophysical research communications Medium 29247648
2017 Electrical pulse stimulation (EPS)-induced GLUT4 translocation in C2C12 myotubes requires Rab8a, Rab13, and Rab14 but not Rab10. Rab10 and Rab13 are not engaged by AMPK activation alone. Constitutively active AS160 diminishes EPS-stimulated GLUT4 translocation, supporting regulation downstream of Rab-GAP AS160. siRNA knockdown; surface HA-GLUT4 measurement; EPS contraction model; constitutively active AS160 expression; AMPK inhibitor and siRNA treatments American journal of physiology. Endocrinology and metabolism Medium 29089333
2019 RAB13 and GGA2 (Golgi-localized gamma ear-containing Arf-binding protein 2) both regulate activity-dependent recycling of active but not inactive beta1-integrin to the plasma membrane. RAB13 silencing triggers intracellular accumulation of active beta1-integrin and reduces integrin activity in focal adhesions and cell migration. GGA2 and RAB13 interact (identified by BioID proximity labeling). RNAi screen; BioID proximity labeling; surface beta1-integrin activity assays; immunofluorescence; cell migration assays; siRNA knockdown Journal of cell science Medium 31076515
2020 Peripheral translation of RAB13 mRNA at cellular protrusions leads to co-translational association of nascent RAB13 with the exchange factor RABIF. This co-translational RAB13-RABIF association at the cell periphery is required for directing RAB13 GTPase activity and promoting efficient cell migration. Preventing RAB13 mRNA localization (without affecting total RAB13 protein levels or membrane association) reduces RAB13 GTPase activation and cell migration efficiency. RNA localization perturbation constructs; RABIF co-translational interaction assays; GTPase activity measurements; cell migration assays; ribosome fractionation The EMBO journal High 32946136
2020 RAB13 mRNA is targeted to sites of filopodia formation via a 192-nt localisation element. This spatial coupling of mRNA localisation, translation, and protein activity creates a polarised domain of filopodia extension. Genomic excision of this localisation element depolarises filopodia dynamics in motile endothelial cells and induces mispatterning of blood vessels in zebrafish without affecting total RAB13 translation. Single-molecule RNA FISH; gene editing (CRISPR); zebrafish live-cell imaging; protrusion-derived RNAseq; filopodia dynamics measurements The EMBO journal High 32946121
2020 Rab13 regulates secretion of small extracellular vesicles (sEVs) in mutant KRAS colorectal cancer cells. Rab13 is both a cargo protein packaged into sEVs and a regulator of sEV secretion. Knockdown of Rab13 blocks proliferative and tumorigenic effects of sEVs on recipient cells. siRNA knockdown; sEV isolation and characterization; recipient cell proliferation and tumorigenesis assays; Western blot Scientific reports Medium 32978434
2022 RNF115 (an E3 ubiquitin ligase) catalyzes K11-linked ubiquitination on Lys46 and Lys58 residues of RAB13. This modification impairs recruitment of GDI1 to RAB13, a prerequisite for RAB reactivation. Knockdown of RAB13 inhibits post-Golgi trafficking of TLRs to the cell surface, and reconstitution of RAB13 with K46/58R mutations (ubiquitination-resistant) in RNF115+/+ cells promotes TLR trafficking from Golgi to cell surface. Co-immunoprecipitation; ubiquitination assays; site-directed mutagenesis (K46R, K58R); siRNA knockdown; TLR trafficking assays; subcellular fractionation; GDI1 recruitment assay Advanced science High 35343654
2022 Rab13 controls membrane translocation of CXCR1/2 receptors, allowing breast cancer stem cells to interact with tumor-associated macrophages and cancer-associated fibroblasts to establish a supportive CSC niche. Rab13 depletion suppresses stemness and chemoresistance. siRNA knockdown; surface CXCR1/2 measurement; co-culture assays; in vivo tumorigenesis assays; flow cytometry Cancer research Medium 35395074
2007 Rab13 and JRAB/MICAL-L2 are required for TPA-induced epithelial cell scattering (MDCK cells). During scattering, Rab13 is transiently activated; both Rab13 and JRAB/MICAL-L2 redistribute from cell-cell contact sites to emerging lamellipodial structures. Knockdown of either Rab13 or JRAB/MICAL-L2 suppresses TPA-induced scattering. siRNA knockdown; Rab13 GTP-loading assay; immunofluorescence; cell scattering assay Oncogene Medium 17891173
2008 RAB13 associates with vinculin and espin (ectoplasmic specialization actin-binding proteins) in the testis, as demonstrated by co-immunoprecipitation and immunofluorescence. RAB13 localizes to the ectoplasmic specialization in Sertoli-germ cell junctions and its level decreases during adjudin-induced ectoplasmic specialization disassembly. Co-immunoprecipitation; immunofluorescence; immunohistochemistry; in vivo adjudin model Biology of reproduction Medium 19074001
2013 RAB13 knockdown in cultured Sertoli cells increases PKA activity, reinforces occludin and filamentous actin distribution at cell-cell interfaces, and promotes ZO-1–occludin direct interaction—effects that phenocopy testosterone's enhancement of TJ integrity. The effects of Rab13 knockdown on TJ permeability are antagonized by PKA inhibition, establishing a Rab13–PKA axis in Sertoli cell TJ regulation. siRNA knockdown in primary cultured Sertoli cells; transepithelial electrical resistance; co-immunoprecipitation; immunofluorescence; PKA activity assay Journal of molecular endocrinology Medium 23419316
2013 JRAB/MICAL-L2 in its open conformational state (JRABΔCC mutant) interacts with filamin isoforms (actin cross-linking proteins) as well as actinin-1 and actinin-4. Filamin but not actinin is required for JRAB-induced cell spreading with membrane ruffles. Degradation of filamins by ASB2 inhibits JRAB-induced cell spreading. Co-immunoprecipitation; expression of constitutively open/closed JRAB mutants; ASB2-induced filamin degradation; morphological cell spreading analysis Genes to cells Medium 23890175
2013 Rab13 and Rab8 both bind to Endospanin-2 (and its homolog Endospanin-1) via the C-terminus of Endospanin-2, as demonstrated by bacterial two-hybrid and colocalization in perinuclear vesicular structures. Rab7, Rab10, Rab11, and Rab32 do not bind Endospanin-2. Bacterial two-hybrid screen; co-immunoprecipitation; immunofluorescence colocalization FEBS open bio Low 23772379
2003 Rab13 mutants specifically impair cell-surface transport of claudin-1 but not basolateral LDLR or apical p75NTR in fibroblasts, while Rab3B mutants affect LDLR but not claudin-1 or p75NTR. This defines distinct and non-overlapping roles for Rab13 and Rab3B in polarized transport. Overexpression of dominant mutant Rabs; cell-surface transport assays for specific cargo proteins in fibroblasts Biochemical and biophysical research communications Medium 12901864
2017 Active Rab13 promotes autophagy in vascular endothelial cells by functionally interacting with Grb2, leading to AMPK activation and mTOR inhibition. Co-immunoprecipitation assays showed Rab13 promotes Rab13–Grb2 interaction; Grb2 knockdown suppresses Rab13-induced autophagy. Overexpression of active/inactive Rab13; siRNA knockdown of Grb2; co-immunoprecipitation; immunofluorescence; autophagy quantification; mTOR/AMPK phosphorylation assays Biochimica et biophysica acta. Molecular cell research Medium 28087344
2024 Parkin (RBR E3 ubiquitin ligase) ubiquitinates RAB13 in cells. This was identified in an orthogonal ubiquitin transfer (OUT) screen and confirmed by reconstituted ubiquitination reactions in vitro and in cells. Mitophagy stimulation enhanced Parkin-mediated ubiquitination of RAB13. Orthogonal ubiquitin transfer (OUT) cascade; proteomics; in vitro reconstituted ubiquitination assay; cell-based ubiquitination assay bioRxivpreprint Medium

Source papers

Stage 0 corpus · 54 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2010 Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells. Proceedings of the National Academy of Sciences of the United States of America 148 21041651
2004 Rab13 mediates the continuous endocytic recycling of occludin to the cell surface. The Journal of biological chemistry 125 15528189
2002 The small GTPase Rab13 regulates assembly of functional tight junctions in epithelial cells. Molecular biology of the cell 106 12058051
2007 The interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions. Molecular biology of the cell 102 18094055
2008 Rab13 regulates membrane trafficking between TGN and recycling endosomes in polarized epithelial cells. The Journal of cell biology 88 18779367
2006 JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin. Molecular biology of the cell 84 16525024
2004 Rab13 regulates PKA signaling during tight junction assembly. The Journal of cell biology 82 15096524
2000 Differentiation of the epithelial apical junctional complex during mouse preimplantation development: a role for rab13 in the early maturation of the tight junction. Mechanisms of development 79 11025210
2015 DENND2B activates Rab13 at the leading edge of migrating cells and promotes metastatic behavior. The Journal of cell biology 71 25713415
2014 Rab35 promotes the recruitment of Rab8, Rab13 and Rab36 to recycling endosomes through MICAL-L1 during neurite outgrowth. Biology open 71 25086062
2009 Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2. Molecular and cellular biology 68 20008558
2014 Rab13 acts downstream of the kinase Mst1 to deliver the integrin LFA-1 to the cell surface for lymphocyte trafficking. Science signaling 61 25074980
1998 The rod cGMP phosphodiesterase delta subunit dissociates the small GTPase Rab13 from membranes. The Journal of biological chemistry 55 9712853
2015 A complex of Rab13 with MICAL-L2 and α-actinin-4 is essential for insulin-dependent GLUT4 exocytosis. Molecular biology of the cell 52 26538022
2011 Rab13-dependent trafficking of RhoA is required for directional migration and angiogenesis. The Journal of biological chemistry 48 21543326
2016 Regulation of Cancer Cell Behavior by the Small GTPase Rab13. The Journal of biological chemistry 45 27044746
2003 Distinct roles of Rab3B and Rab13 in the polarized transport of apical, basolateral, and tight junctional membrane proteins to the plasma membrane. Biochemical and biophysical research communications 43 12901864
2020 Rab13 regulates sEV secretion in mutant KRAS colorectal cancer cells. Scientific reports 41 32978434
2020 RNA localization and co-translational interactions control RAB13 GTPase function and cell migration. The EMBO journal 40 32946136
2019 GGA2 and RAB13 promote activity-dependent β1-integrin recycling. Journal of cell science 39 31076515
2017 Electrical pulse stimulation induces GLUT4 translocation in C2C12 myotubes that depends on Rab8A, Rab13, and Rab14. American journal of physiology. Endocrinology and metabolism 39 29089333
2012 Rab13 small G protein and junctional Rab13-binding protein (JRAB) orchestrate actin cytoskeletal organization during epithelial junctional development. The Journal of biological chemistry 37 23100251
2022 RNF115 Inhibits the Post-ER Trafficking of TLRs and TLRs-Mediated Immune Responses by Catalyzing K11-Linked Ubiquitination of RAB1A and RAB13. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 33 35343654
2007 Involvement of Rab13 and JRAB/MICAL-L2 in epithelial cell scattering. Oncogene 31 17891173
2020 RAB13 mRNA compartmentalisation spatially orients tissue morphogenesis. The EMBO journal 28 32946121
2023 Single-cell transcriptomics in ovarian cancer identify a metastasis-associated cell cluster overexpressed RAB13. Journal of translational medicine 23 37046345
2021 Identification of autophagic target RAB13 with small-molecule inhibitor in low-grade glioma via integrated multi-omics approaches coupled with virtual screening of traditional Chinese medicine databases. Cell proliferation 23 34632655
2022 Rab13 Sustains Breast Cancer Stem Cells by Supporting Tumor-Stroma Cross-talk. Cancer research 22 35395074
2012 Rab13 is upregulated during osteoclast differentiation and associates with small vesicles revealing polarized distribution in resorbing cells. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 22 22562557
2009 Dislocation of Rab13 and vasodilator-stimulated phosphoprotein in inactive colon epithelium in patients with Crohn's disease. International journal of molecular medicine 20 19885626
2008 RAB13 participates in ectoplasmic specialization dynamics in the rat testis. Biology of reproduction 19 19074001
2019 A Novel lncRNA MYOSLID/miR-1286/RAB13 Axis Plays a Critical Role in Osteosarcoma Progression. Cancer management and research 17 31849524
2017 PKC and Rab13 mediate Ca2+ signal-regulated GLUT4 traffic. Biochemical and biophysical research communications 15 29247648
2013 Junctional Rab13-binding protein (JRAB) regulates cell spreading via filamins. Genes to cells : devoted to molecular & cellular mechanisms 15 23890175
2017 Up-regulation of the active form of small GTPase Rab13 promotes macroautophagy in vascular endothelial cells. Biochimica et biophysica acta. Molecular cell research 13 28087344
2016 Rab13 Traffics on Vesicles Independent of Prenylation. The Journal of biological chemistry 12 26969162
2013 RAB13 regulates Sertoli cell permeability barrier dynamics through protein kinase A. Journal of molecular endocrinology 11 23419316
2023 Exosomal proteomics identifies RAB13 as a potential regulator of metastasis for HCC. Hepatology communications 8 36633475
2016 Rab13 Is Involved in the Entry Step of Hepatitis C Virus Infection. Acta medica Okayama 8 27094836
2008 Identification and characterization of JRAB/MICAL-L2, a junctional Rab13-binding protein. Methods in enzymology 8 18413246
2023 Clinical implications of RAB13 expression in pan-cancer based on multi-databases integrative analysis. Scientific reports 6 37803063
2013 Novel interaction of Rab13 and Rab8 with endospanins. FEBS open bio 6 23772379
2023 Construction of Two Independent RAB Family-Based Scoring Systems Based on Machine Learning Algorithms and Definition of RAB13 as a Novel Therapeutic Target for Hepatocellular Carcinoma. International journal of molecular sciences 4 36901767
2021 Rab13 and Desmosome Redistribution in Uterine Epithelial Cells During Early Pregnancy. Reproductive sciences (Thousand Oaks, Calif.) 4 33527312
2005 Assay of Rab13 in regulating epithelial tight junction assembly. Methods in enzymology 4 16473586
2024 Identification and validation of Rab GTPases RAB13 as biomarkers for peritoneal metastasis and immune cell infiltration in colorectal cancer patients. Frontiers in immunology 3 39192986
2024 RAB13 regulates macrophage polarization in sepsis. Scientific reports 3 39223234
2023 A Fluorescent Vector of Carbon Dot to Deliver Rab13 and Rab14 Plasmids for Promoting Neurite Outgrowth. ACS applied bio materials 2 37679053
2024 Role of Rab13, Protein Kinase A, and Zonula Occludens-1 in Hepatitis E Virus Entry and Cell-to-Cell Spread: Comparative Analysis of Quasi-Enveloped and Non-Enveloped Forms. Pathogens (Basel, Switzerland) 1 39770389
2005 Properties of Rab13 interaction with protein kinase A. Methods in enzymology 1 16473634
2026 Single-Cell Profiling Reveals RAB13+ Endothelial Cells and Profibrotic Mesenchymal Cells in Aged Human Bone Marrow. Aging cell 0 41954292
2025 Enteropathogenic E. coli effector Map interacts with Rab13 and regulates the depletion of the tight junction proteins occludin and claudins via cathepsin B-mediated mechanisms. Biology open 0 39912222
2025 Vasoactive intestinal peptide reduces ocular hypertension by regulating tight junction of trabecular meshwork through Rab13/PKA signalling complex. Annals of medicine 0 40859875
2025 MZF1-AS1/NSUN2 promotes the peritoneal metastasis of gastric cancer by enhancing the stability of RAB13 mRNA. Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 0 41343127

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