Affinage

PSMD7

26S proteasome non-ATPase regulatory subunit 7 · UniProt P51665

Round 2 corrected
Length
324 aa
Mass
37.0 kDa
Annotated
2026-04-28
130 papers in source corpus 10 papers cited in narrative 10 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PSMD7 is a non-catalytic MPN-domain subunit of the 26S proteasome 19S regulatory particle lid that forms an obligate heterodimer with the deubiquitinase PSMD14 (Rpn11), stabilizing and activating PSMD14's isopeptidase function during substrate translocation (PMID:24463465, PMID:24516147). The PSMD7 MPN domain contains a degenerate JAMM motif lacking zinc-coordinating residues essential for metalloprotease activity, rendering it catalytically inactive as an isopeptidase and assigning it a primarily structural role within the lid (PMID:17559875). Its yeast ortholog Nas7p is required for proteasome function under stress (PMID:9714768), and PSMD7 interacts with HIV-1 Vpr through its C-terminal domain, linking it to Vpr-induced G2/M arrest (PMID:9520381, PMID:12237292). In cancer contexts, PSMD7 has been reported to deubiquitinate and stabilize RAD23B and SOX2, promoting chemoresistance via the RAD23B–XPC DNA-repair axis and activating Notch1 signaling through SOX2, respectively (PMID:34512150, PMID:38494478).

Mechanistic history

Synthesis pass · year-by-year structured walk · 7 steps
  1. 1998 Medium

    Identification of PSMD7 as a 26S proteasome 19S regulatory subunit and HIV-1 Vpr interactor established its dual role in proteasome biology and viral exploitation of the cell cycle.

    Evidence Yeast two-hybrid, co-immunoprecipitation, yeast NAS7 gene disruption with heat-stress phenotype

    PMID:9520381 PMID:9714768

    Open questions at the time
    • Mechanism by which Vpr–PSMD7 interaction triggers G2/M arrest not defined
    • Whether PSMD7 has proteasome-independent functions in cell-cycle control unclear
  2. 2002 Medium

    Mapping the Vpr-binding determinant to PSMD7's C-terminal domain and linking this region to glucocorticoid receptor–mediated nuclear import revealed a domain-specific mechanism for PSMD7 relocalization.

    Evidence Deletion mutagenesis, co-immunoprecipitation, immunofluorescence with dexamethasone and glucocorticoid antagonist treatments

    PMID:12237292

    Open questions at the time
    • No direct binding between PSMD7 C-terminus and glucocorticoid receptor shown
    • Functional consequence of nuclear relocalization on proteasome activity not tested
  3. 2007 High

    The crystal structure of the PSMD7 MPN domain resolved why it lacks isopeptidase activity: its degenerate JAMM motif cannot coordinate the catalytic zinc ion, establishing PSMD7 as a structural rather than enzymatic MPN subunit.

    Evidence X-ray crystallography at 1.96 Å, size-exclusion chromatography, sequence comparison with catalytically active JAMM domains

    PMID:17559875

    Open questions at the time
    • Structure of full-length PSMD7 not determined
    • Contribution of non-MPN regions to proteasome lid assembly unknown
  4. 2014 High

    High-resolution structures of the PSMD7–PSMD14 (Rpn8–Rpn11) heterodimer demonstrated that PSMD7 is an obligate activating partner of PSMD14, and that full deubiquitinase activation requires incorporation into the 26S proteasome and is coupled to ATP hydrolysis.

    Evidence X-ray crystallography at 2.0 Å, in vitro DUB activity assays, site-directed mutagenesis, EM density docking (two independent studies)

    PMID:24463465 PMID:24516147

    Open questions at the time
    • Conformational dynamics of the insertion-loop gating mechanism not resolved at atomic level in the intact 26S context
    • Contribution of individual PSMD7–PSMD14 interfaces to lid assembly versus DUB activation not dissected
  5. 2018 Medium

    Functional studies in esophageal cancer showed PSMD7 knockdown impairs proteasome function and suppresses mTOR/p70S6K signaling, linking proteasome subunit stoichiometry to growth-factor pathway output.

    Evidence Lentiviral shRNA knockdown and overexpression, phospho-Western blotting, in vivo xenograft

    PMID:29632807

    Open questions at the time
    • Whether mTOR/p70S6K effects are specific to PSMD7 or secondary to general proteasome impairment not distinguished
    • No proteasome activity quantification beyond pathway phospho-readouts
  6. 2021 Medium

    Discovery that PSMD7 directly deubiquitinates and stabilizes RAD23B, thereby maintaining XPC-dependent DNA repair and conferring cisplatin resistance, revealed an unexpected substrate-directed DUB function for a supposedly catalytically inactive MPN subunit.

    Evidence Co-immunoprecipitation, in vivo ubiquitination assay, shRNA knockdown/overexpression, cisplatin sensitivity assays, xenograft

    PMID:34512150

    Open questions at the time
    • Direct isopeptidase activity of PSMD7 on RAD23B not reconstituted with purified recombinant protein
    • Whether DUB activity is intrinsic to PSMD7 or mediated through co-purifying PSMD14 not excluded
    • Not independently replicated
  7. 2024 Medium

    Extension of the DUB paradigm to SOX2 in pancreatic cancer, with epistasis rescue experiments placing PSMD7 upstream of SOX2–Notch1 signaling, broadened the substrate repertoire attributed to PSMD7.

    Evidence Co-immunoprecipitation, ubiquitination assay, SOX2 rescue experiments, Notch1 pathway reporter assays, in vivo xenograft

    PMID:38494478

    Open questions at the time
    • In vitro reconstitution of PSMD7-catalyzed SOX2 deubiquitination not performed
    • Catalytic residues responsible for DUB activity not identified given the degenerate JAMM motif
    • Whether PSMD14 co-purifies in cancer-context immunoprecipitates not controlled

Open questions

Synthesis pass · forward-looking unresolved questions
  • The central unresolved question is whether the reported DUB activity toward RAD23B and SOX2 is intrinsic to PSMD7 or mediated by co-purifying PSMD14, given that structural data show PSMD7 lacks the canonical catalytic zinc site.
  • Reconstitution of purified recombinant PSMD7 DUB activity in vitro is needed
  • Catalytic-site mutagenesis in PSMD7 to abolish reported DUB activity not performed
  • Proteasome-independent versus proteasome-dependent roles of PSMD7 in cancer not delineated

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0140096 catalytic activity, acting on a protein 2
Localization
GO:0005634 nucleus 2 GO:0005829 cytosol 2
Pathway
R-HSA-392499 Metabolism of proteins 4
Partners
HIV-1 VPRPSMD14RAD23BSOX2
Complex memberships
26S proteasome 19S regulatory particle (lid)

Evidence

Reading pass · 10 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 PSMD7 (hVIP/MOV34), a 34-kDa member of the MOV34 family and component of the 26S proteasome 19S regulatory complex, was identified as a direct binding partner of HIV-1 Vpr by yeast two-hybrid assay and confirmed by in vitro and in vivo co-immunoprecipitation. hVIP/MOV34 localizes to the nucleus and its subcellular localization shifts from nuclear to perinuclear upon Vpr-induced G2/M cell-cycle arrest, coinciding with inhibition of MPF-associated histone H1 kinase activity, indicating a functional role in cell-cycle regulation. Yeast two-hybrid, in vitro binding assay, in vivo co-immunoprecipitation, immunofluorescence localization, histone H1 kinase activity assay Proceedings of the National Academy of Sciences of the United States of America Medium 9520381
1998 Human PSMD7 (p40.5) was cloned and identified as a non-ATPase subunit of the PA700 (19S) regulatory complex of the 26S proteasome, homologous to yeast Nas7p. Disruption of the yeast NAS7 gene caused hypersensitivity to heat stress (inability to grow at 37°C), while NAS6 disruption had no effect, demonstrating that Nas7p/PSMD7 is required for normal proteasome function under stress conditions. cDNA cloning, sequence homology analysis, yeast gene disruption with phenotypic readout (heat-stress sensitivity) Gene Medium 9714768
2002 The carboxyl-terminal domain of hVIP/MOV34 (PSMD7) is critical for its interaction with HIV-1 Vpr, as C-terminal deletion mutants abolish both Vpr binding and Vpr-induced nuclear relocalization of hVIP. Full-length hVIP is cytoplasmic but translocates to the nucleus in the presence of Vpr or dexamethasone; C-terminal mutants are unresponsive to both Vpr and glucocorticoids, linking PSMD7's C-terminus to glucocorticoid receptor signaling. Deletion mutagenesis, co-immunoprecipitation, immunofluorescence localization, pharmacological manipulation (dexamethasone/glucocorticoid antagonist) The Journal of biological chemistry Medium 12237292
2007 The crystal structure of the MPN domain of human PSMD7 (Mov34) was solved at 1.96 Å resolution and revealed a nine-β-strand/three-α-helix fold with a domain-swap dimer in solution. Unlike the catalytically active JAMM/MPN+ domains of Rpn11 (PSMD14) and Csn5, the PSMD7 MPN domain lacks the zinc-coordinating residues required for metalloprotease activity (the JAMM motif is degenerate), explaining why PSMD7 has no isopeptidase activity and instead serves a structural role within the 19S proteasome lid. X-ray crystallography (1.96 Å and 3.0 Å), size-exclusion chromatography, dynamic light scattering, amino acid sequence comparison Journal of molecular biology High 17559875
2014 Crystal structures of the yeast Rpn8-Rpn11 MPN-domain heterodimer (the ortholog of human PSMD7-PSMD14) at 2.0 Å revealed that Rpn8 (PSMD7 ortholog) forms two distinct interfaces with the catalytically active Rpn11 (PSMD14 ortholog). Rpn8 is required to stabilize Rpn11 in the heterodimer; structural and mutational analysis showed that Rpn11 lacks a conserved surface for ubiquitin Ile44-patch binding and exhibits no linkage specificity, functioning as a promiscuous deubiquitinase for co-translocation substrate deubiquitination. X-ray crystallography (2.0 Å), in vitro deubiquitinase activity assay, site-directed mutagenesis Nature structural & molecular biology High 24463465
2014 Crystal structures of the Rpn8-Rpn11 heterodimer (human PSMD7-PSMD14 orthologs) showed that full DUB activation of Rpn11 requires its incorporation into the 26S proteasome and is dependent on ATP hydrolysis, suggesting coupling of substrate processing with ubiquitin removal. An insertion segment in Rpn11 acts as a physical barrier across the substrate access channel and a conformationally unstable catalytic loop prevent premature deubiquitination; contacts with ATPase subunits (observed by docking into EM density) stabilize the active conformation. Rpn8 (PSMD7) contributes structurally to forming the active heterodimer. X-ray crystallography, nanobody-assisted crystallization, in vitro DUB activity assay, EM density docking Proceedings of the National Academy of Sciences of the United States of America High 24516147
2009 Global proteomic analysis of deubiquitinase-associated complexes confirmed that PSMD7 (as part of the 19S proteasome lid) is a component of the deubiquitinase interaction landscape, associating with the 26S proteasome complex including PSMD14 and other 19S regulatory particle subunits. Affinity purification–mass spectrometry (AP-MS) with CompPASS scoring Cell Medium 19615732
2018 PSMD7 knockdown in esophageal squamous cell carcinoma (EC9706) cells decreased proliferation, increased apoptosis, reduced proteasomal function, and suppressed mTOR/p70S6K pathway activity (lower p-mTOR Ser2448 and p-p70S6K Thr421/Ser424). Conversely, PSMD7 overexpression in Het-1A cells increased phosphorylation of mTOR and p70S6K, placing PSMD7 upstream of mTOR/p70S6K signaling. In vivo xenograft experiments confirmed decelerated tumor growth upon PSMD7 knockdown with attenuated mTOR/p70S6K signaling. Lentivirus-mediated shRNA knockdown, overexpression, Western blotting for pathway components, cell proliferation/apoptosis assays, in vivo xenograft FEBS open bio Medium 29632807
2021 PSMD7 functions as a deubiquitinase in gastric cancer cells, directly interacting with RAD23B (confirmed by co-immunoprecipitation) and stabilizing RAD23B protein by preventing its ubiquitin-mediated proteasomal degradation. PSMD7 knockdown enhanced RAD23B ubiquitination and degradation, reduced XPC protein levels (a RAD23B-dependent DNA repair factor), suppressed proliferation/invasion, increased cisplatin sensitivity, and inhibited tumor growth in vivo, defining the PSMD7–RAD23B–XPC axis as a mechanism of chemotherapy resistance. Co-immunoprecipitation, ubiquitination assay, shRNA knockdown, overexpression, cell viability/apoptosis/DNA damage repair assays, in vivo xenograft International journal of biological sciences Medium 34512150
2024 PSMD7 deubiquitinates and stabilizes SOX2 protein in pancreatic cancer cells, preventing its proteasomal degradation. Stabilized SOX2 activates the Notch1 signaling pathway, promoting pancreatic cancer cell proliferation and invasion. Restoration of SOX2 expression rescued the antitumor effect of PSMD7 silencing, placing PSMD7 upstream of SOX2–Notch1 in pancreatic cancer progression. In vitro and in vivo gain/loss-of-function assays, co-immunoprecipitation, ubiquitination assay, rescue experiments with SOX2 re-expression, Notch1 pathway reporter analysis Cell & bioscience Medium 38494478

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1996 Structure and functions of the 20S and 26S proteasomes. Annual review of biochemistry 2108 8811196
2002 Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 1924 12167863
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2009 Recognition and processing of ubiquitin-protein conjugates by the proteasome. Annual review of biochemistry 1398 19489727
2009 Defining the human deubiquitinating enzyme interaction landscape. Cell 1282 19615732
2003 Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts. Nature 1236 12808466
2003 DNA deamination mediates innate immunity to retroviral infection. Cell 1150 12809610
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2003 Induction of APOBEC3G ubiquitination and degradation by an HIV-1 Vif-Cul5-SCF complex. Science (New York, N.Y.) 1006 14564014
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2003 The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA. Nature 912 12808465
2013 Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization. Nature 870 23503661
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2004 A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. Nature cell biology 841 14743216
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2003 The antiretroviral enzyme APOBEC3G is degraded by the proteasome in response to HIV-1 Vif. Nature medicine 798 14528300
2003 Species-specific exclusion of APOBEC3G from HIV-1 virions by Vif. Cell 763 12859895
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2007 Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 733 17353931
2004 Increased expression of interleukin 23 p19 and p40 in lesional skin of patients with psoriasis vulgaris. The Journal of experimental medicine 718 14707118
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2003 HIV-1 Vif protein binds the editing enzyme APOBEC3G and induces its degradation. Nature medicine 679 14528301
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2003 HIV-1 Vif blocks the antiviral activity of APOBEC3G by impairing both its translation and intracellular stability. Molecular cell 607 14527406
2011 Global landscape of HIV-human protein complexes. Nature 593 22190034
2018 High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies. Molecular cell 580 29395067
2003 Hypermutation of HIV-1 DNA in the absence of the Vif protein. Science (New York, N.Y.) 570 12750511
1996 The interleukin 12 p40 gene promoter is primed by interferon gamma in monocytic cells. The Journal of experimental medicine 544 8551218
1995 Regulation of interleukin 12 p40 expression through an NF-kappa B half-site. Molecular and cellular biology 413 7565674
1995 Mouse interleukin-12 (IL-12) p40 homodimer: a potent IL-12 antagonist. European journal of immunology 399 7843232
1995 Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity. Journal of immunology (Baltimore, Md. : 1950) 353 7527811
1995 Regulation of interleukin-12 expression in human monocytes: selective priming by interferon-gamma of lipopolysaccharide-inducible p35 and p40 genes. Blood 308 7605994
2011 p40 (ΔNp63) is superior to p63 for the diagnosis of pulmonary squamous cell carcinoma. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 307 22056955
2003 Constitutive p40 promoter activation and IL-23 production in the terminal ileum mediated by dendritic cells. The Journal of clinical investigation 286 12952918
1996 A novel interleukin-12 p40-related protein induced by latent Epstein-Barr virus infection in B lymphocytes. Journal of virology 285 8551575
1993 The interleukin-12 subunit p40 specifically inhibits effects of the interleukin-12 heterodimer. European journal of immunology 275 8103745
1988 Functional and structural characterization of P40, a mouse glycoprotein with T-cell growth factor activity. Proceedings of the National Academy of Sciences of the United States of America 275 3137580
2000 Selective requirement for c-Rel during IL-12 P40 gene induction in macrophages. Proceedings of the National Academy of Sciences of the United States of America 240 11058167
1996 Induction of actinorhodin production by rpsL (encoding ribosomal protein S12) mutations that confer streptomycin resistance in Streptomyces lividans and Streptomyces coelicolor A3(2). Journal of bacteriology 190 8955413
1997 In vivo production and function of IL-12 p40 homodimers. Journal of immunology (Baltimore, Md. : 1950) 184 9127002
2003 The critical regulator of embryonic hematopoiesis, SCL, is vital in the adult for megakaryopoiesis, erythropoiesis, and lineage choice in CFU-S12. Proceedings of the National Academy of Sciences of the United States of America 170 12552125
2014 Structure of the Rpn11-Rpn8 dimer reveals mechanisms of substrate deubiquitination during proteasomal degradation. Nature structural & molecular biology 131 24463465
1999 Amplification and overexpression of p40 subunit of eukaryotic translation initiation factor 3 in breast and prostate cancer. The American journal of pathology 118 10362802
2002 The adaptor protein p40(phox) as a positive regulator of the superoxide-producing phagocyte oxidase. The EMBO journal 117 12456638
2014 Crystal structure of the proteasomal deubiquitylation module Rpn8-Rpn11. Proceedings of the National Academy of Sciences of the United States of America 113 24516147
1999 Mutational analysis of the p63/p73L/p51/p40/CUSP/KET gene in human cancer cell lines using intronic primers. Cancer research 113 10485447
1989 Mutations in ribosomal proteins S4 and S12 influence the higher order structure of 16 S ribosomal RNA. Journal of molecular biology 112 2477554
2014 Activation of epidermal growth factor receptor mediates mucin production stimulated by p40, a Lactobacillus rhamnosus GG-derived protein. The Journal of biological chemistry 105 24895124
2013 Role of anthocyanin-enriched purple-fleshed sweet potato p40 in colorectal cancer prevention. Molecular nutrition & food research 99 23784800
1991 Sequences required for coordinate induction of adeno-associated virus p19 and p40 promoters by Rep protein. Journal of virology 99 2033660
1996 Herpes simplex virus type 1-mediated up-regulation of IL-12 (p40) mRNA expression. Implications in immunopathogenesis and protection. Journal of immunology (Baltimore, Md. : 1950) 98 8557986
2007 Mutational analysis of S12 protein and implications for the accuracy of decoding by the ribosome. Journal of molecular biology 97 17967466
2003 Inhibition of interleukin-12 p40 transcription and NF-kappaB activation by nitric oxide in murine macrophages and dendritic cells. The Journal of biological chemistry 94 14679201
2011 NFIL3 is a regulator of IL-12 p40 in macrophages and mucosal immunity. Journal of immunology (Baltimore, Md. : 1950) 92 21383239
2008 RimO, a MiaB-like enzyme, methylthiolates the universally conserved Asp88 residue of ribosomal protein S12 in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 92 18252828
1998 HIV-1 Vpr interacts with a human 34-kDa mov34 homologue, a cellular factor linked to the G2/M phase transition of the mammalian cell cycle. Proceedings of the National Academy of Sciences of the United States of America 91 9520381
2002 IL-12: the role of p40 versus p75. Scandinavian journal of immunology 86 12100467
2010 Functional analysis of the p40 and p75 proteins from Lactobacillus casei BL23. Journal of molecular microbiology and biotechnology 85 21178363
2005 Mycobacterium tuberculosis lipoarabinomannan-mediated IRAK-M induction negatively regulates Toll-like receptor-dependent interleukin-12 p40 production in macrophages. The Journal of biological chemistry 85 16263713
2006 Preferential production of the IL-12(p40)/IL-23(p19) heterodimer by dendritic cells from human newborns. European journal of immunology 83 16342235
2004 Interleukin-10 inhibits interleukin-12 p40 gene transcription by targeting a late event in the activation pathway. Molecular and cellular biology 82 14993278
1998 cDNA cloning and functional analysis of p28 (Nas6p) and p40.5 (Nas7p), two novel regulatory subunits of the 26S proteasome. Gene 80 9714768
2012 Interaction of two translational components, lysyl-tRNA synthetase and p40/37LRP, in plasma membrane promotes laminin-dependent cell migration. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 75 22751010
1992 The mitochondrial gene encoding ribosomal protein S12 has been translocated to the nuclear genome in Oenothera. Nucleic acids research 74 1454526
1994 Differential expression of mRNA encoding interleukin-12 p35 and p40 subunits in situ. European journal of immunology 72 7925572
2014 IL12/23 p40 inhibition ameliorates Alzheimer's disease-associated neuropathology and spatial memory in SAMP8 mice. Journal of Alzheimer's disease : JAD 71 24047617
1996 Structure and chromosomal location of the mouse interleukin-12 p35 and p40 subunit genes. European journal of immunology 60 8647196
2004 Identification of a novel BRMS1-homologue protein p40 as a component of the mSin3A/p33(ING1b)/HDAC1 deacetylase complex. Biochemical and biophysical research communications 59 15451426
2020 Lactobacillus casei extracellular vesicles stimulate EGFR pathway likely due to the presence of proteins P40 and P75 bound to their surface. Scientific reports 57 33159116
2011 Error-prone and error-restrictive mutations affecting ribosomal protein S12. Journal of molecular biology 57 21575643
2014 The utility of a novel triple marker (combination of TTF1, napsin A, and p40) in the subclassification of non-small cell lung cancer. Human pathology 55 24746197
2012 p40: a p63 isoform useful for lung cancer diagnosis - a review of the physiological and pathological role of p63. Acta cytologica 51 23221041
2010 Anti-p40 antibodies ustekinumab and briakinumab: blockade of interleukin-12 and interleukin-23 in the treatment of psoriasis. Seminars in cutaneous medicine and surgery 51 20430307
1998 Interleukin-12 and its free p40 subunit regulate immune recognition of endometrial cells: potential role in endometriosis. The Journal of clinical endocrinology and metabolism 51 9506747
2014 p40 is the best marker for diagnosing pulmonary squamous cell carcinoma: comparison with p63, cytokeratin 5/6, desmocollin-3, and sox2. Applied immunohistochemistry & molecular morphology : AIMM 50 24805133
2010 Comparative transcriptomics and proteomics of p-hydroxybenzoate producing Pseudomonas putida S12: novel responses and implications for strain improvement. Applied microbiology and biotechnology 50 20449741
2000 Characterization of p40/GPR69A as a peripheral membrane protein related to the lantibiotic synthetase component C. Biochemical and biophysical research communications 47 10944443
2014 Evaluation of napsin A, TTF-1, p63, p40, and CK5/6 immunohistochemical stains in pulmonary neuroendocrine tumors. American journal of clinical pathology 46 25125621
2004 Activation and repression of interleukin-12 p40 transcription by erythroid Kruppel-like factor in macrophages. The Journal of biological chemistry 46 14976188
1992 Sequence similarity between Borna disease virus p40 and a duplicated domain within the paramyxovirus and rhabdovirus polymerase proteins. Journal of virology 46 1404604
2015 IL12B (p40) Gene Polymorphisms Contribute to Ustekinumab Response Prediction in Psoriasis. Dermatology (Basel, Switzerland) 44 26678060
2008 IL-12 p40 homodimer, but not IL-12 p70, induces the expression of IL-16 in microglia and macrophages. Molecular immunology 44 19100623
2017 Antitumor effect of Batf2 through IL-12 p40 up-regulation in tumor-associated macrophages. Proceedings of the National Academy of Sciences of the United States of America 43 28808017
1995 Two markers, IS901-IS902 and p40, identified by PCR and by using monoclonal antibodies in Mycobacterium avium strains. Journal of clinical microbiology 43 7615703
2019 Deficiency of IL12p40 (Interleukin 12 p40) Promotes Ang II (Angiotensin II)-Induced Abdominal Aortic Aneurysm. Arteriosclerosis, thrombosis, and vascular biology 42 30580570
1998 LBP-p40 binds DNA tightly through associations with histones H2A, H2B, and H4. Biochemical and biophysical research communications 42 9878528
2017 Selective neutralization of IL-12 p40 monomer induces death in prostate cancer cells via IL-12-IFN-γ. Proceedings of the National Academy of Sciences of the United States of America 41 29073075
2007 The crystal structure of the human Mov34 MPN domain reveals a metal-free dimer. Journal of molecular biology 41 17559875
2008 Interleukin-12 p40 gene (IL12B) polymorphisms and the risk of cervical cancer in Korean women. European journal of obstetrics, gynecology, and reproductive biology 40 18367309
2018 p40 Immunohistochemistry Is an Excellent Marker in Primary Lung Squamous Cell Carcinoma. Journal of pathology and translational medicine 39 30235512
2004 The L1 retroelement-related p40 protein induces p38delta MAP kinase. Autoimmunity 39 15115313
2013 The central role of protein S12 in organizing the structure of the decoding site of the ribosome. RNA (New York, N.Y.) 38 24152548
2020 High Prevalence and Disease Correlation of Autoantibodies Against p40 Encoded by Long Interspersed Nuclear Elements in Systemic Lupus Erythematosus. Arthritis & rheumatology (Hoboken, N.J.) 37 31342656
2012 Archaeal JAB1/MPN/MOV34 metalloenzyme (HvJAMM1) cleaves ubiquitin-like small archaeal modifier proteins (SAMPs) from protein-conjugates. Molecular microbiology 37 22970855
2011 Cooperation of p40(phox) with p47(phox) for Nox2-based NADPH oxidase activation during Fcγ receptor (FcγR)-mediated phagocytosis: mechanism for acquisition of p40(phox) phosphatidylinositol 3-phosphate (PI(3)P) binding. The Journal of biological chemistry 37 21956105
1999 Role of Mycoplasma penetrans endonuclease P40 as a potential pathogenic determinant. Infection and immunity 37 10456886
1996 Analysis of nuclear localization of laminin binding protein precursor p40 (LBP/p40). Biochemical and biophysical research communications 37 8954992
1986 The enigma of the gene coding for ribosomal protein S12 in the chloroplasts of Nicotiana. Nucleic acids research 36 3945556
2019 Agreement of CK5/6, p40, and p63 immunoreactivity in non-small cell lung cancer. Pathology 34 30798982
2014 p40 is a more specific marker than p63 for cutaneous poorly differentiated squamous cell carcinoma. Journal of cutaneous pathology 32 25263848
1985 Suppressors of temperature-sensitive mutations in a ribosomal protein gene, rpsL (S12), of Escherichia coli K12. Molecular & general genetics : MGG 32 3162077
1994 The S12 ribosomal protein of Podospora anserina belongs to the S19 bacterial family and controls the mitochondrial genome integrity through cytoplasmic translation. The Journal of biological chemistry 31 8195128
2014 Expression of the p40 isoform of p63 has high specificity for cutaneous sarcomatoid squamous cell carcinoma. Journal of cutaneous pathology 30 25263756
2013 IL-20, IL-21 and p40: potential biomarkers of treatment response for ustekinumab. Acta dermato-venereologica 30 22930279
1996 Identification of the active gene coding for the metastasis-associated 37LRP/p40 multifunctional protein. DNA and cell biology 29 8985115
2021 Deubiquitinase PSMD7 promotes the proliferation, invasion, and cisplatin resistance of gastric cancer cells by stabilizing RAD23B. International journal of biological sciences 28 34512150
1998 The induction of apoptosis in HeLa cells by the loss of LBP-p40. Cell death and differentiation 27 10200442
2010 A proteomic and transcriptomic approach reveals new insight into beta-methylthiolation of Escherichia coli ribosomal protein S12. Molecular & cellular proteomics : MCP 25 21169565
2008 Role of cytokine p40 family in multiple sclerosis. Minerva medica 25 18431321
2024 Deubiquitinase PSMD7 facilitates pancreatic cancer progression through activating Nocth1 pathway via modifying SOX2 degradation. Cell & bioscience 24 38494478
2017 p40 & thyroid transcription factor-1 immunohistochemistry: A useful panel to characterize non-small cell lung carcinoma-not otherwise specified (NSCLC-NOS) category. The Indian journal of medical research 24 29168459
2002 Carboxyl terminus of hVIP/mov34 is critical for HIV-1-Vpr interaction and glucocorticoid-mediated signaling. The Journal of biological chemistry 24 12237292
2001 Down-regulation of IL-12 p40 gene in Plasmodium berghei-infected mice. Journal of immunology (Baltimore, Md. : 1950) 24 11418654
2015 Sigma receptor 1 activation attenuates release of inflammatory cytokines MIP1γ, MIP2, MIP3α, and IL12 (p40/p70) by retinal Müller glial cells. Journal of neurochemistry 23 25439327
2007 Modulation of 16S rRNA function by ribosomal protein S12. Biochimica et biophysica acta 23 17512991
2007 RIP2 mediates LPS-induced p38 and IkappaBalpha signaling including IL-12 p40 expression in human monocyte-derived dendritic cells. European journal of immunology 23 17578844
2003 Regulation of interleukin 12 p40 and p70 production by blood and alveolar phagocytes during severe sepsis. Laboratory investigation; a journal of technical methods and pathology 23 13679443
2018 Correlation between IL-17A/F, IL-23, IL-35 and IL-12/-23 (p40) levels in peripheral blood lymphocyte cultures and disease activity in Behcet's patients. Clinical rheumatology 22 29557072
2014 Interplay of the bacterial ribosomal A-site, S12 protein mutations and paromomycin binding: a molecular dynamics study. PloS one 22 25379961
2008 A signal relay between ribosomal protein S12 and elongation factor EF-Tu during decoding of mRNA. RNA (New York, N.Y.) 22 19095621
2001 A study on the expression of interleukin (IL)-10 and IL-12 P35, P40 mRNA in the psoriatic lesions. Journal of Tongji Medical University = Tong ji yi ke da xue xue bao 22 11523260
2014 The utility of p63, p40, and GATA-binding protein 3 immunohistochemistry in diagnosing micropapillary urothelial carcinoma. Human pathology 21 24993315
2018 PSMD7 downregulation induces apoptosis and suppresses tumorigenesis of esophageal squamous cell carcinoma via the mTOR/p70S6K pathway. FEBS open bio 20 29632807
2020 A combination of cytokeratin 5/6, p63, p40 and MUC5AC are useful for distinguishing squamous cell carcinoma from adenocarcinoma of the cervix. Diagnostic pathology 19 32843061
2012 Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40. Veterinary parasitology 19 23021260
1992 Nonintegrin laminin receptors in the nervous system: evidence for lack of a relationship to P40. Journal of neurobiology 19 1279113