Affinage

PSMC2

26S proteasome regulatory subunit 7 · UniProt P35998

Round 2 corrected
Length
433 aa
Mass
48.6 kDa
Annotated
2026-04-28
130 papers in source corpus 17 papers cited in narrative 17 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PSMC2 is one of six AAA-ATPase subunits of the 19S regulatory particle of the 26S proteasome, where it drives ATP-dependent unfolding and translocation of ubiquitinated substrates into the 20S catalytic core for degradation (PMID:8500623, PMID:8811196, PMID:19489727). Within the 19S base, PSMC2 forms a specific heterodimer with ATPase S4 (PSMC6) and assembles into larger subcomplexes with non-ATPase subunits S2 and S5b through distinct N-terminal and C-terminal interaction domains (PMID:10625621). Through its role in proteasome-dependent protein turnover, PSMC2 controls IκBα degradation and NF-κB activation in microglia (PMID:22629310) and regulates proliferation, apoptosis, and migration in multiple cancer types via downstream effectors including CCND1, PLAU, ITGA6, and the PI3K/Akt and mTOR pathways (PMID:34244472, PMID:34294689, PMID:34413286, PMID:35256584). PSMC2 also exists in lighter-than-26S complexes that associate with basal RNA polymerase II transcription factors TBP, TFIIB, TFIIH, and TFIIF, indicating a proteasome-independent role in transcriptional regulation (PMID:11118327).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 1993 High

    Identification of PSMC2 as an integral 26S proteasome ATPase resolved the identity of subunit 7 and linked it to the previously cloned HIV Tat modulator MSS1, establishing its dual nomenclature and placing it within the proteasome.

    Evidence N-terminal and internal peptide sequencing of purified 26S protease subunit 7 from human erythrocytes

    PMID:8500623

    Open questions at the time
    • Specific enzymatic contribution of PSMC2 versus other ATPase subunits was not defined
    • Stoichiometry and arrangement within the ATPase ring was unknown
  2. 1996 High

    Biochemical reconstitution of the 19S regulatory complex established that PSMC2 functions as one of six ATPases responsible for substrate recognition and unfolding, providing the mechanistic framework for its role in proteasomal degradation.

    Evidence Subunit composition analysis and functional assays of purified 26S proteasome

    PMID:8811196

    Open questions at the time
    • Direct contacts between PSMC2 and neighboring ATPases were not mapped
    • Individual ATPase cycle contribution of PSMC2 was undefined
  3. 2000 High

    Mapping of PSMC2's assembly interactions revealed it forms a specific heterodimer with ATPase S4 and ternary complexes with S2 and S5b through distinct domains, establishing how the 19S base is built from defined subassemblies.

    Evidence Co-translation, co-immunoprecipitation, sedimentation analysis, and chimeric domain-mapping in reticulocyte lysate

    PMID:10625621

    Open questions at the time
    • Structural basis of the S4–S7 heterodimer was not resolved at atomic level
    • Whether the tetramer intermediate is obligate for in vivo assembly was not tested
  4. 2000 Medium

    Detection of PSMC2 in sub-26S complexes with basal Pol II transcription factors (TBP, TFIIB, TFIIH, TFIIF) suggested a proteasome-independent transcriptional role, opening a second functional axis for the protein.

    Evidence Glycerol gradient sedimentation and co-immunoprecipitation from rat tissues and mammalian cells

    PMID:11118327

    Open questions at the time
    • No reciprocal pull-down or reconstitution of PSMC2–transcription factor complexes was performed
    • Functional consequences on transcription were not directly measured
    • Whether this association requires ATPase activity is unknown
  5. 2009 High

    Comprehensive mechanistic analysis confirmed that the six-ATPase ring including PSMC2 provides the driving force for substrate unfolding and ATP-dependent threading into the 20S catalytic chamber, establishing the consensus model of 19S function.

    Evidence Review consolidating ATPase mutant studies and substrate processing assays across multiple labs

    PMID:19489727

    Open questions at the time
    • Individual contributions of PSMC2 versus other ring ATPases to the sequential hydrolysis cycle remained unresolved
    • No high-resolution structure of the human PSMC2-containing ring existed at this time
  6. 2012 Medium

    PSMC2 knockdown in microglia stabilized IκBα and blocked NF-κB activation, directly linking PSMC2's proteasomal function to neuroinflammatory signaling and demonstrating a specific biological output of 26S-dependent degradation.

    Evidence siRNA knockdown in microglial cells with Western blot for IκBα/NF-κB and ELISA for inflammatory mediators

    PMID:22629310

    Open questions at the time
    • Whether the effect is specific to PSMC2 or generalizable to any 19S ATPase knockdown was not addressed
    • In vivo neuroinflammation models were not tested
  7. 2013 Medium

    N-terminal methylation of the yeast PSMC2 ortholog Rpt1 was identified and shown to affect growth and stress tolerance, revealing a post-translational modification layer that tunes proteasome function.

    Evidence Mass spectrometry of purified yeast Rpt1, site-directed mutagenesis, and growth/stress assays

    PMID:24038880

    Open questions at the time
    • Whether human PSMC2 undergoes equivalent N-terminal methylation was not examined
    • Mechanistic link between methylation and proteasome activity or assembly is unclear
  8. 2017 Medium

    Loss-of-function studies in osteosarcoma identified PSMC2 as a driver of proliferation, survival, and migration, with microarray revealing ITGA6, CCND1, CCNE2, and TGFβR2 as downstream transcriptional targets — the first cancer-type delineation of PSMC2-dependent gene programs.

    Evidence shRNA knockdown, MTT/colony assays, flow cytometry, Transwell invasion, xenograft models, and gene microarray with Western blot validation

    PMID:27888613

    Open questions at the time
    • Whether downstream gene changes reflect direct proteasomal substrate stabilization or indirect signaling was not distinguished
    • No proteasome activity assay was performed alongside phenotypic readouts
  9. 2019 Low

    Extension of PSMC2 cancer phenotyping to pancreatic cancer showed conserved pro-survival and anti-apoptotic roles, but without specific pathway placement, leaving the downstream mechanism in this tissue unresolved.

    Evidence shRNA knockdown, MTT, Celigo, Annexin V FACS, caspase-3/7 assay in pancreatic cancer cells

    PMID:31598166

    Open questions at the time
    • No downstream pathway or target identification was attempted
    • Single lab with limited mechanistic depth
    • No rescue or epistasis experiments
  10. 2021 Medium

    A wave of cancer-type studies collectively defined PSMC2's pro-tumorigenic mechanisms through distinct downstream axes: PLAU in breast cancer, CCND1 in ovarian cancer, ITGA6 in hepatocellular carcinoma, Akt/Cyclin D1/CDK6 in prostate cancer, and Wnt pathway suppression in melanoma — demonstrating context-dependent effector selection.

    Evidence Co-immunoprecipitation (PLAU, ITGA6), rescue/epistasis experiments (CCND1 overexpression), Western blot pathway analysis, protein arrays, MG132 pharmacological mimicry, and in vivo xenograft models across five cancer types

    PMID:33902600 PMID:34244472 PMID:34294689 PMID:34413286 PMID:34716318

    Open questions at the time
    • Whether PLAU and ITGA6 are direct proteasomal substrates of PSMC2-containing proteasomes or indirect effectors is unknown
    • No study compared PSMC2 knockdown to general proteasome inhibition to parse PSMC2-specific effects
    • All studies from single labs without independent replication
  11. 2022 Medium

    PSMC2 was shown to regulate RPS15A/mTOR signaling in gastric cancer by competing for hsa-let-7c-3p binding, revealing a non-canonical ceRNA mechanism independent of proteasome catalytic function, alongside additional cancer phenotyping in OSCC and glioma.

    Evidence Dual-luciferase reporter assays for miRNA binding, RPS15A rescue, Torin1 pharmacological inhibition, xenografts, apoptosis antibody array, GSEA

    PMID:34979867 PMID:35256584 PMID:35287689

    Open questions at the time
    • The ceRNA mechanism for PSMC2 transcript is unprecedented and requires independent validation
    • Whether PSMC2 mRNA levels are sufficient to meaningfully sequester let-7c-3p in physiological settings is unaddressed
    • Glioma data lack mechanistic depth beyond GSEA inference

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include whether PSMC2's transcriptional associations with Pol II factors are functionally consequential, whether its pro-tumorigenic effects are truly distinct from general proteasome inhibition, and what the structural basis of PSMC2-specific substrate processing is in the human 26S proteasome.
  • No functional transcription assay has been performed with PSMC2 manipulation
  • No comparison of PSMC2-specific knockdown versus other 19S ATPase knockdowns in any cancer model
  • High-resolution cryo-EM of human PSMC2 in the context of substrate engagement has not been reported in this timeline

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140657 ATP-dependent activity 3 GO:0098772 molecular function regulator activity 2 GO:0140096 catalytic activity, acting on a protein 2
Localization
GO:0005829 cytosol 3 GO:0005654 nucleoplasm 1
Pathway
R-HSA-162582 Signal Transduction 5 R-HSA-392499 Metabolism of proteins 5 R-HSA-5357801 Programmed Cell Death 4 R-HSA-168256 Immune System 1
Partners
GTF2BGTF2H1ITGA6PLAUPSMC6PSMD1PSMD5TBP
Complex memberships
19S regulatory particle26S proteasome

Evidence

Reading pass · 17 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1993 PSMC2 (subunit 7 of the human 26S protease) was identified by peptide sequencing as identical to MSS1, a previously described modulator of HIV Tat-mediated transactivation, establishing it as an integral ATPase component of the 26S proteasome regulatory complex. N-terminal and internal peptide sequencing by CNBr cleavage and direct Edman degradation of purified 26S protease subunit 7 from human erythrocytes FEBS letters High 8500623
1996 PSMC2 (MSS1/S7) functions as one of six ATPases in the 19S regulatory complex (RC) of the 26S proteasome, which is responsible for recognizing and unfolding ubiquitinated protein substrates for degradation by the 20S core particle. Biochemical reconstitution, subunit composition analysis, and functional assays of purified 26S proteasome Annual review of biochemistry High 8811196
2000 PSMC2 (S7) forms a specific heterodimeric pair with the ATPase S4 within the 19S regulatory complex; additionally, non-ATPase subunits S2 and S5b each form ternary complexes with the S4-S7 pair, assembling into a tetramer. The C-terminal two-thirds of PSMC2 mediates binding to S2, while its N-terminal region mediates binding to S5b. In vitro co-translation in reticulocyte lysate, co-immunoprecipitation, sedimentation analysis, and chimeric ATPase domain-mapping experiments The Journal of biological chemistry High 10625621
2000 PSMC2 (MSS1) exists in cellular complexes lighter than the 26S proteasome and associates with multiple basal transcription factors for RNA polymerase II, including TBP, TFIIB, TFIIH, and TFIIF, suggesting a role in transcriptional regulation distinct from its proteasomal function. Glycerol gradient sedimentation, immunoblotting, and co-immunoprecipitation of endogenous complexes from rat tissues and mammalian cells Biochemical and biophysical research communications Medium 11118327
2009 PSMC2 is required for efficient recognition and translocation of ubiquitinated substrates into the 20S proteasome core; together with the other five ATPases in the 19S particle, it forms a ring complex that drives substrate unfolding and ATP-dependent threading into the catalytic chamber. Comprehensive biochemical and genetic analysis reviewed, including ATPase mutant studies and substrate processing assays Annual review of biochemistry High 19489727
2012 PSMC2 knockdown in microglia inhibits IκBα degradation, suppresses NF-κB activation, and reduces LPS-induced production of pro-inflammatory mediators (iNOS, nitric oxide, COX-2, prostaglandin E2), establishing a role for PSMC2 in controlling neuroinflammatory signaling via the ubiquitin-proteasome system. RNA interference knockdown in microglial cells, Western blot for IκBα/NF-κB, ELISA and colorimetric assays for inflammatory mediators PloS one Medium 22629310
2013 N-terminal Pro residue of yeast Rpt1 (the PSMC2 ortholog) undergoes mono- or di-methylation after removal of the initiator Met; deletion of the adjacent Pro-Lys (PK) sequence at positions 3–4 abolishes N-methylation, decreases cell growth, and increases stress sensitivity, implicating this modification in proteasome function. Mass spectrometry analysis of purified yeast Rpt1, site-directed mutagenesis, and growth/stress phenotype assays in yeast Proteomics Medium 24038880
2017 PSMC2 knockdown in osteosarcoma cells suppresses cell proliferation, enhances apoptosis, induces G2/M and S phase arrest, and inhibits migration and invasion in vitro and tumorigenicity in vivo; gene microarray after knockdown identifies ITGA6, FN1, CCND1, CCNE2, and TGFβR2 as downstream targets whose expression is regulated by PSMC2. RNA interference, MTT/colony assays, flow cytometry, transwell invasion assay, nude mouse xenografts, gene microarray, and Western blot validation Oncotarget Medium 27888613
2021 PSMC2 promotes breast cancer progression by directly interacting with plasminogen activator urokinase (PLAU); PSMC2 knockdown suppresses proliferation, apoptosis resistance, and migration in vitro and in vivo, and the PSMC2/PLAU axis was identified as the mechanistic basis by gene microarray and Ingenuity Pathway Analysis. Immunohistochemistry, shRNA knockdown, co-immunoprecipitation (direct interaction with PLAU), gene microarray with IPA, xenograft mouse models Cell death & disease Medium 34244472
2021 PSMC2 promotes ovarian cancer development through regulation of CCND1; knockdown of PSMC2 decreases CCND1 expression, and overexpression of CCND1 reverses PSMC2 knockdown-induced suppression of proliferation and motility, demonstrating a mutual regulatory relationship between PSMC2 and CCND1. shRNA knockdown, gene microarray, overexpression rescue experiments, colony formation, motility assays, and xenograft models Cell death & disease Medium 34294689
2021 PSMC2 directly interacts with ITGA6 in hepatocellular carcinoma cells; PSMC2 knockdown phenocopies ITGA6 knockdown (impaired proliferation, colony formation, migration, enhanced apoptosis), and combined knockdown of both is additive, establishing a PSMC2/ITGA6 functional axis. Co-immunoprecipitation demonstrating direct interaction, gene microarray, shRNA knockdown, functional cell assays, and in vivo xenograft experiments Cell death discovery Medium 34413286
2021 PSMC2 knockdown in prostate cancer cells inhibits proliferation and migration while promoting apoptosis and cell cycle arrest; mechanistically, the regulatory effect is mediated through the Akt/Cyclin D1/CDK6 signaling pathway. qPCR, Western blot, shRNA knockdown, Celigo cell counting, colony formation, wound healing, Transwell assays, flow cytometry, and xenograft models Cancer cell international Medium 33902600
2022 PSMC2 promotes gastric cancer progression via induction of RPS15A and downstream mTOR pathway activation; PSMC2 and RPS15A competitively bind hsa-let-7c-3p (a miRNA), thereby elevating RPS15A levels. RPS15A overexpression rescues the growth inhibition caused by PSMC2 knockdown, and mTOR inhibitor Torin1 reverses RPS15A-promoted proliferation. GeneChip microarray, IPA, dual-luciferase reporter assays for miRNA binding, RPS15A overexpression rescue, Torin1 pharmacological inhibition, in vivo xenograft Oncogenesis Medium 35256584
2022 PSMC2 knockdown in oral squamous cell carcinoma promotes apoptosis through upregulation of pro-apoptotic proteins and downregulation of p100, p-Akt, and CDK6, while upregulating MAPK9, indicating that PSMC2 suppresses apoptosis via the PI3K/Akt pathway. Lentiviral shRNA knockdown, human apoptosis antibody array, Western blot, flow cytometry, MTT, colony formation, Transwell assays, xenograft model Cell cycle Medium 34979867
2022 PSMC2 knockdown in glioma cells inhibits proliferation and promotes apoptosis; gene-set enrichment analysis of PSMC2 co-expression genes indicates PSMC2 affects the apoptosis pathway, with changes in apoptosis-related proteins confirmed after knockdown. siRNA knockdown, CCK-8 proliferation assay, flow cytometry, Western blot, GSEA of co-expression networks World journal of surgical oncology Low 35287689
2021 PSMC2 knockdown in skin cutaneous melanoma cells elevates pro-apoptotic proteins DR6, IGFBP-4, p21, and p53 while inhibiting anti-apoptotic TRAILR-3 and suppressing Wnt signaling pathway proteins, revealing that PSMC2 promotes melanoma progression through Wnt pathway regulation. Protein-chip technology, GSEA, flow cytometry, in vitro proliferation/migration/DNA damage assays, and in vivo xenograft; MG132 pharmacological mimicry and PSMC2 overexpression confirmation Cell death discovery Medium 34716318
2019 PSMC2 knockdown in pancreatic cancer cells inhibits cell proliferation and enhances apoptosis (including caspase-3/7 activation), supporting a role for PSMC2 in sustaining cancer cell survival. shRNA knockdown, MTT assay, Celigo assay, Annexin V FACS, Caspase-3/7 array Journal of Cancer Low 31598166

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1996 Structure and functions of the 20S and 26S proteasomes. Annual review of biochemistry 2108 8811196
2005 Towards a proteome-scale map of the human protein-protein interaction network. Nature 2090 16189514
2002 Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 1924 12167863
2012 Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell 1718 22658674
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2009 Recognition and processing of ubiquitin-protein conjugates by the proteasome. Annual review of biochemistry 1398 19489727
2010 Network organization of the human autophagy system. Nature 1286 20562859
2009 Defining the human deubiquitinating enzyme interaction landscape. Cell 1282 19615732
2003 Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts. Nature 1236 12808466
2003 DNA deamination mediates innate immunity to retroviral infection. Cell 1150 12809610
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2003 Induction of APOBEC3G ubiquitination and degradation by an HIV-1 Vif-Cul5-SCF complex. Science (New York, N.Y.) 1006 14564014
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2003 The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA. Nature 912 12808465
2013 Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization. Nature 870 23503661
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2004 A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. Nature cell biology 841 14743216
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2003 The antiretroviral enzyme APOBEC3G is degraded by the proteasome in response to HIV-1 Vif. Nature medicine 798 14528300
2003 Species-specific exclusion of APOBEC3G from HIV-1 virions by Vif. Cell 763 12859895
2007 Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 733 17353931
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2003 HIV-1 Vif protein binds the editing enzyme APOBEC3G and induces its degradation. Nature medicine 679 14528301
2015 Gene essentiality and synthetic lethality in haploid human cells. Science (New York, N.Y.) 657 26472760
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2003 HIV-1 Vif blocks the antiviral activity of APOBEC3G by impairing both its translation and intracellular stability. Molecular cell 607 14527406
2007 Ribosomal protein S7 as a novel modulator of p53-MDM2 interaction: binding to MDM2, stabilization of p53 protein, and activation of p53 function. Oncogene 220 17310983
2009 Ribosomal protein S7 is both a regulator and a substrate of MDM2. Molecular cell 180 19683495
1994 CD43 (S7) expression identifies peripheral B cell subsets. Journal of immunology (Baltimore, Md. : 1950) 112 7989752
1988 Assembly of the 30S subunit from Escherichia coli ribosomes occurs via two assembly domains which are initiated by S4 and S7. Biochemistry 108 2461734
1998 Detection and assignment of proteins encoded by rice black streaked dwarf fijivirus S7, S8, S9 and S10. The Journal of general virology 102 9634092
1975 Specific cross-linking of proteins S7 and L4 to ribosomal RNA, by UV irradiation of Escherichia coli ribosomal subunits. Molecular & general genetics : MGG 102 814400
1993 Peptide sequencing identifies MSS1, a modulator of HIV Tat-mediated transactivation, as subunit 7 of the 26 S protease. FEBS letters 90 8500623
1983 The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane. Nucleic acids research 74 6338481
1976 The isolation of eukaryotic ribosomal proteins. The purification and characterization of the 40 S ribosomal subunit proteins S2, S3, S4, S5, S6, S7, S8, S9, S13, S23/S24, S27, and S28. The Journal of biological chemistry 72 947902
1993 MSS1, a nuclear-encoded mitochondrial GTPase involved in the expression of COX1 subunit of cytochrome c oxidase. Journal of molecular biology 69 8392589
1988 Probing the assembly of the 3' major domain of 16 S ribosomal RNA. Quaternary interactions involving ribosomal proteins S7, S9 and S19. Journal of molecular biology 67 3373531
2005 Structural locations and functional roles of new subsites S5, S6, and S7 in memapsin 2 (beta-secretase). Biochemistry 66 15628850
2015 Mutation in mitochondrial ribosomal protein S7 (MRPS7) causes congenital sensorineural deafness, progressive hepatic and renal failure and lactic acidemia. Human molecular genetics 65 25556185
1975 Nucleotide sequences of Escherichia coli 16-S RNA associated with ribosomal proteins S7, S9, S10, S14 and S19. European journal of biochemistry 64 1100389
1997 The structure of ribosomal protein S7 at 1.9 A resolution reveals a beta-hairpin motif that binds double-stranded nucleic acids. Structure (London, England : 1993) 62 9331418
1984 The genes for the ribosomal proteins S12 and S7 are clustered with the gene for the EF-Tu protein on the chloroplast genome of Euglena gracilis. Nucleic acids research 62 6324129
2013 Small ribosomal protein subunit S7 suppresses ovarian tumorigenesis through regulation of the PI3K/AKT and MAPK pathways. PloS one 61 24244431
2011 Ling Zhi-8 mediates p53-dependent growth arrest of lung cancer cells proliferation via the ribosomal protein S7-MDM2-p53 pathway. Carcinogenesis 61 21983128
1975 Neutron scattering measurements of separation and shape of proteins in 30S ribosomal subunit of Escherichia coli: S2-S5, S5-S8, S3-S7. Proceedings of the National Academy of Sciences of the United States of America 59 1105567
1997 Ribosomal protein S7: a new RNA-binding motif with structural similarities to a DNA architectural factor. Structure (London, England : 1993) 57 9331423
1987 RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S7, S9, S10, S11, S17, S18 and S21 by treatment with bis-(2-chloroethyl)-methylamine. Nucleic acids research 54 2437528
2000 Mapping subunit contacts in the regulatory complex of the 26 S proteasome. S2 and S5b form a tetramer with ATPase subunits S4 and S7. The Journal of biological chemistry 53 10625621
1987 RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S5, S7, S8, S9, S11, S13, S19 and S21 by treatment with methyl p-azidophenyl acetimidate. Nucleic acids research 53 2437527
1976 Studies on the environment of protein S7 within the 30-S subunit Escherichia coli ribosomes. European journal of biochemistry 53 776616
2007 Molecular phylogenetics of the family Cyprinidae (Actinopterygii: Cypriniformes) as evidenced by sequence variation in the first intron of S7 ribosomal protein-coding gene: further evidence from a nuclear gene of the systematic chaos in the family. Molecular phylogenetics and evolution 52 18203625
1996 Primary structure of ribosomal proteins S3 and S7 from Manduca sexta. Insect molecular biology 52 8630533
2000 Phylogenetic relationships of bluetongue viruses based on gene S7. Virus research 49 10867193
1981 Identification of ribosomal protein S7 as a repressor of translation within the str operon of E. coli. Cell 46 7016341
1994 Post-transcriptional regulation of the str operon in Escherichia coli. Ribosomal protein S7 inhibits coupled translation of S7 but not its independent translation. Journal of molecular biology 45 7507167
1990 Genes for the plastid elongation factor Tu and ribosomal protein S7 and six tRNA genes on the 73 kb DNA from Astasia longa that resembles the chloroplast DNA of Euglena. Molecular & general genetics : MGG 43 2338940
1989 Genes for the ribosomal proteins S12 and S7 and elongation factors EF-G and EF-Tu of the cyanobacterium, Anacystis nidulans: structural homology between 16S rRNA and S7 mRNA. Molecular & general genetics : MGG 43 2499762
2017 A Novel Quorum-Quenching N-Acylhomoserine Lactone Acylase from Acidovorax sp. Strain MR-S7 Mediates Antibiotic Resistance. Applied and environmental microbiology 40 28455333
2011 Knockdown of ribosomal protein S7 causes developmental abnormalities via p53 dependent and independent pathways in zebrafish. The international journal of biochemistry & cell biology 40 21550419
1993 Interaction of Escherichia coli ribosomal protein S7 with 16S rRNA. Nucleic acids research 39 7681943
2003 A functional interaction between ribosomal proteins S7 and S11 within the bacterial ribosome. The Journal of biological chemistry 38 12937172
1980 Effect of ultraviolet irradiation on 30-S ribosomal subunits. Identification of the RNA region crosslinked to protein S7. European journal of biochemistry 37 6989601
2006 Fusion of carbohydrate binding modules from Thermotoga neapolitana with a family 10 xylanase from Bacillus halodurans S7. Extremophiles : life under extreme conditions 34 17006740
1994 Post-transcriptional regulation of the str operon in Escherichia coli. Structural and mutational analysis of the target site for translational repressor S7. Journal of molecular biology 34 8289236
2020 Circular RNA S-7 promotes ovarian cancer EMT via sponging miR-641 to up-regulate ZEB1 and MDM2. Bioscience reports 33 32667627
2000 Phylogenetic analysis of the S7 gene does not segregate Chinese strains of bluetongue virus into a single topotype. Archives of virology 33 10948989
1989 Sequence analysis of the peptide-elongation factor EF-2 gene, downstream from those of ribosomal proteins H-S12 and H-S7, from the archaebacterial extreme halophile, Halobacterium halobium. European journal of biochemistry 32 2513185
2017 PSMC2 is up-regulated in osteosarcoma and regulates osteosarcoma cell proliferation, apoptosis and migration. Oncotarget 31 27888613
1993 Characterization of the S7 ribosomal protein gene in wheat mitochondria. Molecular & general genetics : MGG 31 8437584
1999 Identification of a mammalian mitochondrial homolog of ribosomal protein S7. Biochemical and biophysical research communications 30 10581179
1998 Nuclear import and nucleolar accumulation of the human ribosomal protein S7 depends on both a minimal nuclear localization sequence and an adjacent basic region. Biochemical and biophysical research communications 30 9731210
2020 Ribosomal protein S7 ubiquitination during ER stress in yeast is associated with selective mRNA translation and stress outcome. Scientific reports 29 33184379
2004 Assembly of the 30S ribosomal subunit: positioning ribosomal protein S13 in the S7 assembly branch. RNA (New York, N.Y.) 29 15525707
1991 A paradigm for local conformational control of function in the ribosome: binding of ribosomal protein S19 to Escherichia coli 16S rRNA in the presence of S7 is required for methylation of m2G966 and blocks methylation of m5C967 by their respective methyltransferases. Nucleic acids research 29 1766869
1987 Nucleotide sequence, promoter analysis, and linkage mapping of the unusually organized operon encoding ribosomal proteins S7 and S12 in maize chloroplast. The Journal of biological chemistry 29 2822717
1996 A functional role for some Fugu introns larger than the typical short ones: the example of the gene coding for ribosomal protein S7 and snoRNA U17. Nucleic acids research 28 8774896
1981 Apparent association constants for E. coli ribosomal proteins S4, S7, S8, S15, S17 and S20 binding to 16S RNA. Nucleic acids research 28 7029472
2021 Overexpression of PSMC2 promotes the tumorigenesis and development of human breast cancer via regulating plasminogen activator urokinase (PLAU). Cell death & disease 27 34244472
2011 Molecular characterization of segments S7 to S10 of a southern rice black-streaked dwarf virus isolate from maize in northern China. Virologica Sinica 27 21331890
1992 Photochemical cross-linking of the anticodon loop of yeast tRNA(Phe) to 30S-subunit protein S7 at the ribosomal A and P sites. Biochimie 27 1637863
2022 PSMC2 promotes the progression of gastric cancer via induction of RPS15A/mTOR pathway. Oncogenesis 26 35256584
2010 Indirect ELISA and indirect immunofluorescent antibody assay for detecting the antibody against murine norovirus S7 in mice. Experimental animals 26 20224169
2000 Tissue and cell distribution of a mammalian proteasomal ATPase, MSS1, and its complex formation with the basal transcription factors. Biochemical and biophysical research communications 26 11118327
1989 Analysis of lymphoid population in scid mice; detection of a potential B lymphocyte progenitor population present at normal levels in scid mice by three color flow cytometry with B220 and S7. Current topics in microbiology and immunology 26 2478339
2018 The structure of INI1/hSNF5 RPT1 and its interactions with the c-MYC:MAX heterodimer provide insights into the interplay between MYC and the SWI/SNF chromatin remodeling complex. The FEBS journal 25 30222246
1992 The yeast ribosomal protein S7 and its genes. The Journal of biological chemistry 25 1737755
1989 Ribosomal proteins S7 and L1 are located close to the decoding site of E. coli ribosome--affinity labeling studies with modified tRNAs carrying photoreactive probes attached adjacent to the 3'-end of the anticodon. Nucleic acids research 25 2685749
2000 Tagging ribosomal protein S7 allows rapid identification of mutants defective in assembly and function of 30 S subunits. Journal of molecular biology 24 10772857
2006 Cloning, sequence analysis, and expression of a gene encoding an endoxylanase from Bacillus halodurans S7. Molecular biotechnology 23 16757802
1998 Matching the crystallographic structure of ribosomal protein S7 to a three-dimensional model of the 16S ribosomal RNA. RNA (New York, N.Y.) 23 9582096
1994 Mutational and structural analysis of the RNA binding site for Escherichia coli ribosomal protein S7. Journal of molecular biology 23 7525976
1984 Comparative electron microscopic study on the location of ribosomal proteins S3 and S7 on the surface of the E. coli 30S subunit using monoclonal and conventional antibody. Molecular & general genetics : MGG 23 6394951
2019 PSMC2 is Up-regulated in Pancreatic Cancer and Promotes Cancer Cell Proliferation and Inhibits Apoptosis. Journal of Cancer 22 31598166
2013 Ribosomal protein S7 regulates arsenite-induced GADD45α expression by attenuating MDM2-mediated GADD45α ubiquitination and degradation. Nucleic acids research 22 23563151
2001 Ribosomal protein S7 from Escherichia coli uses the same determinants to bind 16S ribosomal RNA and its messenger RNA. Nucleic acids research 22 11160889
2021 PSMC2/CCND1 axis promotes development of ovarian cancer through regulating cell growth, apoptosis and migration. Cell death & disease 21 34294689
2007 Molecular analysis of the genome segments S1, S4, S6, S7 and S12 of a Rice gall dwarf virus isolate from Thailand; completion of the genomic sequence. Archives of virology 21 17426918
1983 Electron microscopic investigations on the location of rat liver ribosomal proteins S3a, S5, S6, S7 and S9 by means of antibody labeling. Biomedica biochimica acta 21 6196023
2001 Chloroplast ribosomal protein S7 of Chlamydomonas binds to chloroplast mRNA leader sequences and may be involved in translation initiation. The Plant cell 20 11158540
2021 INI1/SMARCB1 Rpt1 domain mimics TAR RNA in binding to integrase to facilitate HIV-1 replication. Nature communications 19 33980829
1997 Specific interactions of the autoantigen L7 with multi-zinc finger protein ZNF7 and ribosomal protein S7. The Journal of biological chemistry 19 9268371
1997 Rice ragged stunt oryzavirus genome segments S7 and S10 encode non-structural proteins of M(r) 68,025 (Pns7) and M(r) 32,364 (Pns10). Archives of virology 19 9672632
2021 PSMC2/ITGA6 axis plays critical role in the development and progression of hepatocellular carcinoma. Cell death discovery 18 34413286
1986 Multiple crosslinks of proteins S7, S9, S13 to domains 3 and 4 of 16S RNA in the 30S particle. Nucleic acids research 18 2423965
2019 Identification of a novel membrane-associated protein from the S7 segment of grass carp reovirus. The Journal of general virology 17 30688636
2000 Proteins S7, S10, S16 and S19 of the human 40S ribosomal subunit are most resistant to dissociation by salt. Biochimica et biophysica acta 17 11121577
2013 N-Terminal methylation of proteasome subunit Rpt1 in yeast. Proteomics 16 24038880
2003 The S7 gene and VP7 protein are highly conserved among temporally and geographically distinct American isolates of epizootic hemorrhagic disease virus. Virus research 16 12902042
1988 Protein binding sites on Escherichia coli 16S ribosomal RNA; RNA regions that are protected by proteins S7, S9 and S19, and by proteins S8, S15 and S17. Nucleic acids research 16 3279390
1987 Protein binding sites on Escherichia coli 16S RNA; RNA regions that are protected by proteins S7, S14 and S19 in the presence or absence of protein S9. Nucleic acids research 16 2438658
2022 PSMC2 is overexpressed in glioma and promotes proliferation and anti-apoptosis of glioma cells. World journal of surgical oncology 15 35287689
2021 Cellular Trafficking of Glutathione Transferase M2-2 Between U373MG and SHSY-S7 Cells is Mediated by Exosomes. Neurotoxicity research 14 33555546
1995 The phosphorylated ribosomal protein S7 in Tetrahymena is homologous with mammalian S4 and the phosphorylated residues are located in the C-terminal region. Structural characterization of proteins separated by two-dimensional polyacrylamide gel electrophoresis. The Journal of biological chemistry 14 7890730
1980 Analysis of rpsD mutations in Escherichia coli. IV. Accumulation of minor forms of protein S7(K) in ribosomes of rpsD mutant strains due to translational read-through. Molecular & general genetics : MGG 14 6990204
1979 The primary structure of ribosomal protein S7 from E. coli strains K and B. Biochimie 14 385062
2022 PSMC2 knockdown inhibits the progression of oral squamous cell carcinoma by promoting apoptosis via PI3K/Akt pathway. Cell cycle (Georgetown, Tex.) 13 34979867
2021 Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration. Cancer cell international 13 33902600
2009 Phylogenetic relationships of North American phoxinins (Actinopterygii: Cypriniformes: Leuciscidae) as inferred from S7 nuclear DNA sequences. Molecular phylogenetics and evolution 13 19909821
1991 The nucleotide sequences of Bacillus stearothermophilus ribosomal protein S12 and S7 genes: comparison with the str operon of Escherichia coli. Agricultural and biological chemistry 13 1368664
2020 A novel biphenyl compound IMB-S7 ameliorates hepatic fibrosis in BDL rats by suppressing Sp1-mediated integrin αv expression. Acta pharmacologica Sinica 12 31932644
2017 BCCIPβ modulates the ribosomal and extraribosomal function of S7 through a direct interaction. Journal of molecular cell biology 12 28510697
1990 The primary structure of rat ribosomal protein S7. FEBS letters 12 2226813
2022 BmCPV-Derived Circular DNA vcDNA-S7 Mediated by Bombyx mori Reverse Transcriptase (RT) Regulates BmCPV Infection. Frontiers in immunology 11 35371040
2021 PSMC2 knockdown suppressed tumor progression of skin cutaneous melanoma. Cell death discovery 11 34716318
2012 Inhibition of 26S protease regulatory subunit 7 (MSS1) suppresses neuroinflammation. PloS one 11 22629310
1995 Inactivation of the UAS1 of STA1 by glucose and STA10 and identification of two loci, SNS1 and MSS1, involved in STA10-dependent repression in Saccharomyces cerevisiae. Molecular & general genetics : MGG 11 7700227