| 2004 |
PNN (Pinn/DRS) physically interacts with the transcriptional corepressor CtBP1 via in vitro pull-down and in vivo co-immunoprecipitation, and this interaction relieves CtBP1-mediated repression of the E-cadherin promoter, indicating PNN can modulate CtBP1 transcriptional repressor activity. |
In vitro pull-down, co-immunoprecipitation, immunofluorescence, overexpression and RNAi experiments, E-cadherin promoter reporter assay |
Molecular and cellular biology |
High |
15542832
|
| 2003 |
PNN associates preferentially with spliced mRNAs (not unspliced pre-mRNA) at a position immediately upstream of the splice junction, interacts with the exon-exon junction complex component RNPS1, and participates in mRNA splicing and nuclear export; PNN is nuclear-restricted as shown by heterokaryon assay, and overexpression of its N-terminal RNPS1-binding fragment blocks pre-mRNA splicing. |
Immunoprecipitation of mRNPs, oligonucleotide-directed RNase H digestion, heterokaryon assay, overexpression/knockdown with poly(A)+ RNA nuclear export assay |
Molecular and cellular biology |
High |
14517304
|
| 2003 |
PNN interacts with SR proteins SRp75, SRm300, and the novel SR-rich protein SRrp130 via its polyserine/RS motif, as demonstrated by yeast two-hybrid, co-immunoprecipitation, and co-localization in nuclear speckles of corneal epithelial cells, suggesting PNN is part of a multiprotein complex involved in pre-mRNA processing. |
Yeast two-hybrid, co-immunoprecipitation, co-immunostaining, domain mapping |
Investigative ophthalmology & visual science |
High |
14578391
|
| 2007 |
CtBP recruits PNN to CtBP-associated chromatin complexes, resulting in PNN-dependent chromatin remodeling at the E-cadherin promoter; additionally, CtBP and PNN differentially modulate E-cadherin mRNA splicing with RNA Polymerase II serving as an interface, establishing a transcription-coupled mRNA splicing mechanism. |
Chromatin immunoprecipitation, co-immunoprecipitation, mRNA splicing analysis, RNA Pol II interaction assays |
Molecular and cellular biology |
High |
18086895
|
| 2012 |
PNN depletion causes cellular apoptosis through SRSF1-mediated alternative splicing changes: loss of PNN reduces SRSF1 levels, which shifts splicing of Bcl-x toward pro-apoptotic Bcl-xS and alters ICAD splicing; overexpression of SRSF1 rescues apoptosis and restores Bcl-xL and functionless ICAD production. PNN knockout in mice results in early embryonic lethality. |
RNAi knockdown in MCF-7 cells, RT-PCR for alternative splicing, rescue by SRSF1 overexpression, mouse knockout |
Journal of cell science |
High |
22454513
|
| 2005 |
RNAi-mediated knockdown of PNN in human corneal epithelial cells leads to loss of cell-cell adhesion, downregulation of E-cadherin, desmoglein, desmoplakin, ZO-1, and keratin anchorage to desmosomes, altered SR protein distribution, and cell escape from the epithelium; rescue with a conservatively mutated PNN construct confirms specificity. |
shRNAi knockdown, Western blot, immunofluorescence, rescue experiment with mutated PNN construct |
Molecular vision |
High |
15735603
|
| 2000 |
Pinin localizes at or near the desmosome in quiescent corneal epithelial cells, and its association with desmosomes is greatly reduced within 2 hours of wounding while desmoplakin remains cell boundary-associated; overexpression of PNN inhibits epithelial cell migration after wounding, driving cells to a hyperstable adhesive state. |
Immunofluorescence and immunoelectron microscopy of wounded corneas, transfection of full-length PNN cDNA with migration assay |
Investigative ophthalmology & visual science |
High |
10798648
|
| 2009 |
Conditional inactivation of Pnn in the developing surface eye ectoderm (Pax6-Cre) causes loss of corneal epithelial identity (loss of K12, ectopic K10/K14), squamous metaplasia correlated with elevated β-catenin activity and Tcf4 levels, and misregulated p68 RNA helicase, demonstrating PNN is required for corneal epithelial differentiation and modulates Wnt/β-catenin signaling. |
Conditional knockout mouse (Cre-lox), histology, immunohistochemistry for keratins, β-catenin, Tcf4, p68 |
Investigative ophthalmology & visual science |
High |
19892877
|
| 2013 |
PNN and ESRP1 physically associate in protein complexes and co-localize in/around nuclear speckles in corneal epithelial cells; genome-wide transcriptome array analysis reveals that PNN knockdown alters alternative splicing of a specific subset of genes (including PAX6(5a), FOXJ3, ARHGEF11, SLC37A2) regulating epithelial morphology and movement, distinct from but overlapping with ESRP1-regulated targets. |
Co-immunoprecipitation, deconvolution microscopy, inducible shRNA knockdown, whole-transcriptome array, RT-PCR validation |
Investigative ophthalmology & visual science |
High |
23299472
|
| 2017 |
PNN is upregulated during early reprogramming of somatic cells to iPSCs; PNN co-localizes with spliceosomal components in pluripotent stem cell nuclear speckles, physically interacts with SNRPA1 and SNRPD1, and its depletion causes pronounced loss of pluripotency and blocks hiPS generation, establishing PNN as a key regulator of pluripotency-specific spliceosome assembly. |
Gene expression profiling, interaction network analysis, co-immunoprecipitation, co-localization, shRNA knockdown with pluripotency assays |
Stem cell research |
Medium |
28595116
|
| 2014 |
PNN regulates alternative splicing of a specific subset of long non-coding RNAs (including Has2as/HAS2-AS1 and RP11-series lncRNAs) in corneal epithelial cells, as shown by RT-PCR quantification of splice variants in PNN knockdown HCET cells and Pnn-deficient mouse corneas, with lncRNA splice variant localization confirmed by in situ hybridization. |
RT-PCR, in situ hybridization with variant-specific probes, Pnn knockout mouse cornea, shRNA knockdown |
Molecular vision |
Medium |
25489234
|
| 2000 |
The PNN gene locus maps to chromosome 14q13 within a known tumor suppressor locus (D14S75-D14S288); re-expression of full-length PNN cDNA in J82 and EcR-293 cells inhibits anchorage-independent growth; PNN CpG island methylation correlates with reduced expression in tumor tissues. |
FISH, Northern blot, soft agar anchorage-independent growth assay, methylation analysis |
Oncogene |
Medium |
10645008
|
| 2022 |
Neuron-specific Pnn deficiency in mice increases oxidative stress markers (elevated NOX-1, NOX-2, oxidized proteins), alters SRSF1/SRSF2 balance (reduced SRSF1, increased SRSF2), increases DNA damage marker p53bp1, and exacerbates cerebral ischemia/reperfusion injury (larger infarct, elevated pro-apoptotic proteins) following MCAO. |
Inducible neuron-specific conditional knockout mouse, middle cerebral artery occlusion (MCAO), Western blot, immunofluorescence |
Antioxidants (Basel, Switzerland) |
Medium |
35326115
|
| 2020 |
In neurons under oxygen-glucose deprivation (OGD), PNN expression increases during OGD and decreases during reoxygenation, with cytoplasmic translocation of PNN observed (nuclear-to-cytoplasmic redistribution); in astrocytes, PNN expression decreases during OGD and increases during reoxygenation without cytoplasmic translocation, indicating cell-type-specific responses to ischemic stress. |
Primary cultured rat neurons and astrocytes with OGD, MCAO mouse model, immunofluorescence, Western blot |
Brain sciences |
Medium |
33027948
|
| 1999 |
The memA/DRS protein (PNN) is identical to a 160 kDa nuclear protein found in the nucleoplasm and in U2-snRNP structures, contains three coiled-coil domains and a glycine loop domain, and is elevated in highly metastasizing human melanoma cell lines compared to non-metastasizing ones. |
Subtractive hybridization, sequence analysis, expression analysis across cell lines |
Biochimica et biophysica acta |
Low |
10095061
|