Affinage

PIF1

ATP-dependent DNA helicase PIF1 · UniProt Q9H611

Length
641 aa
Mass
69.8 kDa
Annotated
2026-06-10
100 papers in source corpus 60 papers cited in narrative 61 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PIF1 is an ATP-dependent, 5'→3' SF1 DNA helicase that safeguards genome stability by resolving non-canonical nucleic acid structures and protein barriers encountered during DNA replication and repair (PMID:1849081, PMID:8253734, PMID:23596008). Its core catalytic mechanism is now defined in detail: it translocates on ssDNA with a one-base-pair kinetic step at ~0.84 ATP per nucleotide (PMID:23596008), operates as a monomer that dimerizes upon DNA binding (PMID:20795654), and unwinds duplex DNA by a branched mechanism dominated by a repetitive, short-range opening mode (PMID:31744872); crystal structures of bacterial, yeast, and human Pif1 orthologs reveal a wedge-mediated strand-separation mechanism, a conserved ssDNA-binding channel, and a dedicated G4-recognizing surface (PMID:26904952, PMID:30698796, PMID:35736675). A central function is the recognition and resolution of G-quadruplex (G4) DNA, which it unwinds more efficiently than B-DNA and dismantles stepwise through a G-triplex intermediate; loss of this activity causes replication stalling, breakage, and instability at G4-forming loci, a function conserved from bacteria to humans (PMID:19424434, PMID:21620135, PMID:23657261, PMID:25471447). During DNA synthesis PIF1 stimulates Pol δ-mediated strand-displacement and migrating D-loop formation in break-induced replication and Okazaki fragment maturation, acts with Dna2 in long-flap processing, and enables the replisome to bypass R-loops and tightly bound proteins such as Rap1 by simultaneously displacing protein and nucleic-acid barriers (PMID:16537895, PMID:18689797, PMID:24025768, PMID:27001517, PMID:33199603). These genome-maintenance roles are coordinated through a non-canonical PCNA-interacting motif required for Pol δ stimulation and recruitment to damage and meiotic recombination sites, with its activity restrained by the Mer3-MutLβ complex competing for PCNA binding (PMID:29141206, PMID:33823531). PIF1 also negatively regulates telomerase by directly removing it from telomeric DNA, preferentially acting at long telomeres (PMID:8287473, PMID:16522649, PMID:25906395), and is targeted to both mitochondria and nucleus via distinct N- and C-terminal signals, where it is essential for mtDNA maintenance and nuclear genome stability (PMID:1849081, PMID:17827721). In human cells, PIF1 supports replication fork progression and S-phase checkpoint signaling, and a breast-cancer-associated L319P mutant is selectively defective in break-induced replication (PMID:33470420, PMID:25359767).

Mechanistic history

Synthesis pass · year-by-year structured walk · 19 steps
  1. 1991 High

    Established the founding biochemical identity of PIF1 as a DNA-unwinding enzyme and placed it in mitochondria, defining the molecular activity all later work would build on.

    Evidence ImmunoEM and in vivo import plus in vitro ATPase/helicase assays with purified protein

    PMID:1849081 PMID:8253734

    Open questions at the time
    • Nuclear role not yet addressed
    • Physiological substrates unidentified at this stage
  2. 1994 High

    Answered what nuclear process PIF1 controls by revealing it as a negative regulator of telomerase, the first defined genome-maintenance function.

    Evidence Loss-of-function genetics with telomere Southern blotting and HO-induced DSB de novo telomere assays in yeast

    PMID:8287473

    Open questions at the time
    • Direct biochemical mechanism of telomerase inhibition not shown
    • Relevance to human PIF1 unknown
  3. 2006 Medium

    Resolved how PIF1 inhibits telomerase mechanistically and showed the activity is conserved in human cells, while distinguishing mitochondrial versus nuclear isoform functions.

    Evidence In vitro telomerase displacement/processivity assays, isoform genetics, and ectopic helicase-dead-controlled expression in HT1080 cells

    PMID:16522649 PMID:16935874 PMID:17130244

    Open questions at the time
    • Mouse Pif1 showed no telomere phenotype, indicating species divergence
    • Single primary source for direct displacement mechanism
  4. 2006 High

    Defined a second nuclear role in Okazaki fragment processing, placing PIF1 in a genetic pathway with Dna2 and Pol δ for long-flap maturation.

    Evidence Genetic epistasis and synthetic lethality suppression in S. cerevisiae

    PMID:16537895

    Open questions at the time
    • Biochemical mechanism of flap generation not yet reconstituted at this point
  5. 2008 High

    Reconstituted the OFP mechanism, showing PIF1 accelerates long-flap growth to channel processing into the Dna2/FEN1 two-nuclease pathway.

    Evidence Reconstituted in vitro Okazaki fragment processing with purified yeast proteins

    PMID:18689797 PMID:19605347 PMID:20959454

    Open questions at the time
    • In vivo balance between FEN1 and Pif1/Dna2 pathways not fully quantified
  6. 2009 High

    Identified G-quadruplex DNA as a key physiological substrate, linking PIF1 catalytic activity to genome stability at G4-forming sequences.

    Evidence In vitro G4 unwinding with purified protein plus in vivo genetic rescue by G4-ablating CEB1 mutations; human forked-substrate and domain analyses

    PMID:19424434 PMID:19700773 PMID:20524933

    Open questions at the time
    • Genome-wide scope of G4 binding not yet established
    • Strand specificity of action unresolved
  7. 2011 High

    Extended G4 resolution to the genome scale, demonstrating PIF1 binds G4 motifs in vivo and prevents replication stalling and breakage there.

    Evidence Genome-wide ChIP, replication and breakage assays, and spontaneous suppressor analysis in yeast

    PMID:21620135

    Open questions at the time
    • Recruitment determinants to G4 sites in vivo not defined
  8. 2013 High

    Defined the catalytic mechanism quantitatively and established conservation, showing a 1-bp step active helicase and that human PIF1 functionally complements yeast G4 and telomere phenotypes.

    Evidence Pre-steady-state kinetics, single-molecule translocation assays, and cross-species complementation

    PMID:23446274 PMID:23596008 PMID:23657261

    Open questions at the time
    • Monomer-versus-dimer functional roles only partially resolved
    • Atomic structure not yet available
  9. 2013 High

    Connected PIF1 to recombination-associated DNA synthesis, showing it drives migrating D-loop extension by Pol δ during break-induced replication.

    Evidence In vitro D-loop extension with purified Pif1/Pol δ/Rad51 and BIR genetic assays in yeast

    PMID:24025768

    Open questions at the time
    • Recruitment mechanism to D-loops not yet defined
  10. 2014 High

    Resolved the single-molecule mechanism of structure resolution, revealing a patrolling monomer that repetitively unfolds G4 one strand at a time and unwinds RNA:DNA hybrids.

    Evidence smFRET/TIRF single-molecule analysis with purified ScPif1

    PMID:24843019

    Open questions at the time
    • How patrolling integrates into the replisome in vivo not shown
  11. 2014 Medium

    Identified checkpoint phosphorylation as a regulatory input, linking the Mec1-Rad53 cascade to PIF1's BIR and telomere functions, and defined a tumor-cell dependency on PIF1.

    Evidence Phosphorylation-site mutant genetics in yeast; siRNA knockdown with DNA fiber and CHK1 analysis in human tumor cells

    PMID:21616935 PMID:25329304 PMID:25359767

    Open questions at the time
    • Direct phosphosite mapping limited in some studies
    • Tumor-selective apoptosis mechanism incompletely defined
  12. 2016 High

    Demonstrated protein-displacement activity in vivo and in vitro, showing PIF1 clears bound proteins like Rap1 ahead of Pol δ and is needed for lagging-strand replication through G4s via a PCNA interaction.

    Evidence Reconstituted strand-displacement assays, live-cell single-fork imaging, and PIP-box mutant analysis

    PMID:26733194 PMID:27001517 PMID:30395308

    Open questions at the time
    • Strand asymmetry of requirement not mechanistically explained at structural level
  13. 2016 High

    Provided the first structural framework for the unwinding mechanism, defining the wedge strand-separator and conformational changes from bacterial Pif1 crystal structures.

    Evidence X-ray crystallography of BaPif1 with ADP-AlF4 and ssDNA plus mutational validation

    PMID:26904952

    Open questions at the time
    • G4-bound structure not yet captured
    • Human-specific structural divergence unresolved at this stage
  14. 2017 High

    Established the structural and functional basis of PCNA-coupled activity, defining a non-canonical PIP motif required for Pol δ stimulation, BIR, and replication through tRNA genes and R-loops.

    Evidence Crystal structure of ScPif1-PCNA, mutant strand-displacement and BIR assays, and ChIP-seq with R-loop manipulation

    PMID:28429714 PMID:29141206 PMID:30107417

    Open questions at the time
    • How PCNA loading is timed relative to barrier encounter not fully defined
  15. 2018 High

    Defined regulatory and disease-relevant determinants, including acetylation control, the essential signature motif, and the L319P/L430P mutation that uncouples unwinding from G4 binding.

    Evidence MS site mapping with biochemical assays; signature-motif and disease-mutant mutagenesis in yeast and S. pombe; human BIR reporter analysis of L319P

    PMID:30053106 PMID:30239884 PMID:32878983 PMID:33470420

    Open questions at the time
    • Physiological triggers of acetylation in vivo not defined
    • Human disease causality beyond functional defect not established in timeline
  16. 2019 High

    Captured human PIF1 structures and the branched unwinding mechanism, and showed PIF1's role in replication termination, R-loop/protein barrier bypass, and resection at G4-prone sites.

    Evidence Multiple human PIF1 crystal structures with mutagenesis; smFRET branched-mechanism analysis; reconstituted fork convergence and SSB-cooperation assays; human DSB resection assays

    PMID:30232007 PMID:30698796 PMID:30850330 PMID:31340040 PMID:31744872 PMID:31772234

    Open questions at the time
    • In vivo significance of human structural divergence not tested
    • Resection partner identities only partially defined
  17. 2020 High

    Demonstrated direct replisome-level barrier bypass, showing PIF1 simultaneously strips a dCas9-bound R-loop, and clarified RPA cooperation and FEN1 control of PIF1 entry.

    Evidence Reconstituted yeast replisome with single-molecule visualization; reconstituted strand displacement through nucleosome and transcription-factor barriers; RPA co-precipitation and CEB1 genetics

    PMID:32190820 PMID:32913126 PMID:33199603

    Open questions at the time
    • How PIF1 distinguishes barriers requiring removal from those bypassed by SSBs not fully resolved
  18. 2021 High

    Refined PIF1's recombination role to long-track BIR synthesis and showed it is actively restrained during meiosis by Mer3-MutLβ competing for PCNA.

    Evidence BIR-versus-gene-conversion reporters with L319P analysis and synthetic lethality; in vitro D-loop inhibition, Co-IP, and meiotic ChIP-seq

    PMID:33470420 PMID:33823531

    Open questions at the time
    • Regulation of the PCNA-competition switch in mitotic versus meiotic contexts not defined
  19. 2022 High

    Resolved how PIF1 recognizes G4 at atomic resolution, defining a G4-recognizing surface that engages the intact quadruplex without disrupting its topology.

    Evidence X-ray crystallography of ToPif1-G4 complex

    PMID:35736675

    Open questions at the time
    • Coupling of G4 recognition to ATP-driven unfolding not captured structurally
    • Human GRS equivalence not directly demonstrated

Open questions

Synthesis pass · forward-looking unresolved questions
  • How human PIF1's nuclear and mitochondrial activities are coordinated in vivo, and whether its tumor-cell dependency and disease-associated variants can be exploited therapeutically, remain open.
  • In vivo human substrate spectrum not mapped
  • Mechanistic basis of tumor-selective lethality unresolved
  • Causal disease link beyond functional defects not established

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 4 GO:0098772 molecular function regulator activity 4 GO:0140097 catalytic activity, acting on DNA 4 GO:0016787 hydrolase activity 3 GO:0140657 ATP-dependent activity 3
Localization
GO:0005739 mitochondrion 3 GO:0005634 nucleus 2
Pathway
R-HSA-69306 DNA Replication 4 R-HSA-73894 DNA Repair 4 R-HSA-1640170 Cell Cycle 2 R-HSA-1474165 Reproduction 1
Partners
DNA2MER3PCNAPOL32RAP1RIM1RPATERT

Evidence

Reading pass · 61 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1991 PIF1 gene product localizes to mitochondria (confirmed by immunoelectron microscopy and in vivo import experiments) and functions as a single-stranded DNA-dependent ATPase and 5' to 3' DNA helicase that unwinds partial DNA duplexes. Immunoelectron microscopy, in vivo import assay using ts mas1 mutants, in vitro ATPase and helicase assays with purified overexpressed protein The EMBO journal High 1849081
1993 Purified ScPif1 is a distributive 5' to 3' DNA helicase: it specifically utilizes ATP or dATP with MgCl2, requires ssDNA as ATPase effector, is stimulated by forked substrates, and exists as a monomer (sedimentation coefficient 6.5 S) in solution. Purification from mitochondria to near-homogeneity; in vitro ATPase assay, DNA helicase assay, sedimentation analysis The Journal of biological chemistry High 8253734
1994 ScPif1 helicase inhibits both telomere elongation and de novo telomere formation: pif1 mutations cause all telomeres to lengthen and elevate de novo telomere addition at double-strand breaks, establishing Pif1 as a negative regulator of telomerase. Genetic screen for subtelomeric gene silencing loss; telomere Southern blotting; HO-induced DSB de novo telomere formation assay in pif1 mutants Cell High 8287473
2006 ScPif1 inhibits telomerase by directly removing telomerase RNA template from telomeric DNA in vitro; the nuclear isoform (pif1-m2) specifically affects telomere functions, while the mitochondrial isoform affects mtDNA maintenance. In vitro telomerase displacement assay; genetic separation of mitochondrial vs. nuclear isoforms Nucleic acids research Medium 16935874
2006 ScPif1 functions in Okazaki fragment processing (OFP) together with Dna2 helicase/nuclease: deletion of PIF1 suppresses lethality of DNA2-null mutants, and further deletion of POL32 (Pol δ subunit) suppresses remaining phenotypes, placing Pif1 in a pathway with Dna2 and Pol δ for long-flap processing. Genetic epistasis; synthetic lethality suppression; double and triple deletion analysis in S. cerevisiae Molecular and cellular biology High 16537895
2006 Murine Pif1 (mPif1) physically associates with telomerase in vivo, but mPif1 knockout mice show no telomere length alteration and mPif1 does not affect telomerase elongation activity in vitro, indicating the telomerase-inhibitory function of yeast Pif1 is not conserved in mice. mPif1 gene disruption (knockout mice); telomere Southern blotting; Co-IP of mPif1 with telomerase; in vitro telomerase extension assay Molecular and cellular biology Medium 17130244
2006 Human PIF1 (hPif1) inhibits telomerase activity: wild-type but not ATPase/helicase-deficient hPif1 causes telomere shortening in HT1080 cells; hPif1 reduces telomerase processivity and unwinds DNA/RNA duplex in vitro; hPif1 preferentially binds telomeric DNA in vitro and in vivo. Ectopic overexpression in HT1080 cells; telomere Southern blot; in vitro telomerase processivity assay; DNA/RNA duplex unwinding assay; ChIP for telomeric DNA binding Nucleic acids research High 16522649
2007 Human PIF1 has at least two isoforms from alternative splicing; the N-terminal region contains a mitochondrial targeting signal and the C-terminal region contains a nuclear localization signal; siRNA knockdown of hPif1 causes S-phase delay. Deletion mutant analysis; fluorescence localization in HeLa cells; siRNA knockdown with cell-cycle analysis by flow cytometry Biological & pharmaceutical bulletin Medium 17827721
2008 ScPif1 accelerates long flap growth during Okazaki fragment maturation, promoting RPA binding to long flaps, which inhibits FEN1 and directs processing toward the Dna2/FEN1 two-nuclease pathway. Reconstituted in vitro Okazaki fragment processing system with purified yeast proteins and model substrates; flap displacement and ligation assays The Journal of biological chemistry High 18689797
2008 Human PIF1 N-terminal domain (PINT domain) enhances ssDNA binding and is required for efficient DNA unwinding; hPif1 contains a strand annealing activity residing in the PINT domain; both activities are inhibited by RPA. Deletion mutant analysis; ssDNA-dependent ATPase assays; helicase unwinding assays; strand annealing assay; RPA inhibition assay Nucleic acids research Medium 18835853
2009 ScPif1 promotes long flap formation by Pol δ during Okazaki fragment processing to create substrates that require Dna2 cleavage; Dna2 reverses RPA-mediated inhibition of ligation in the reconstituted system. Reconstituted in vitro Okazaki fragment processing with purified proteins and model substrates The Journal of biological chemistry High 19605347
2009 Human PIF1 specifically recognizes and unwinds DNA structures resembling stalled replication forks, requiring both arms of the fork for efficient unwinding; the conserved core helicase domain (hPifHD) possesses strand annealing activity. In vitro DNA binding and helicase assays on forked and duplex substrates; domain deletion analysis Nucleic acids research Medium 19700773
2009 ScPif1 absence promotes genomic instability at G4-forming CEB1 minisatellite sequences; Pif1 protein unwinds G4 structures more efficiently than regular B-DNA in vitro; mutations eliminating G4-forming potential of CEB1 abolish instability in pif1Δ cells. Genetic instability assay in S. cerevisiae; in vitro G4 unwinding assay with purified Pif1; G4 structure formation confirmed by CD spectroscopy and chemical probing PLoS genetics High 19424434
2010 Human PIF1 is a G4 DNA-binding and unwinding (resolvase) protein; G4 DNA unwinding requires an extended (>10 nt) 5' ssDNA tail; the conserved helicase domain (hPifHD) is sufficient for both G4 binding and unwinding activities. In vitro G4 DNA binding assays; G4 unwinding assays; competition assays; domain deletion analysis The Biochemical journal Medium 20524933
2010 DNA binding induces dimerization of ScPif1: while Pif1 is a monomer in solution, ssDNA binding induces protein dimerization, also observed on tailed- and forked-dsDNA substrates; dimer forms on unwinding substrates in the presence of non-hydrolyzable ATP analogues, suggesting the dimer is the pre-initiation complex. Analytical ultracentrifugation; fluorescence anisotropy; gel shift assays with purified recombinant ScPif1 and various DNA substrates Biochemistry High 20795654
2010 ScPif1 is an alternative pathway factor for Okazaki fragment processing of fold-back flaps: Pif1 working with Pol δ unwinds full-length Okazaki fragments initiated by fold-back flaps, providing an alternative to the FEN1/Dna2 pathway. Biochemical reconstitution with purified proteins and model Okazaki fragment substrates; fold-back flap substrates The Journal of biological chemistry Medium 20959454
2010 Pif1 and Exo1 function downstream of Cdc13 telomere capping to resect uncapped telomeric DNA: in cdc13-1 cells, Pif1 and Exo1 resect telomeric DNA within 5 kb, stimulating weak checkpoint activation; further resection by Exo1 alone (>5 kb) causes full checkpoint activation. Genetic epistasis using cdc13-1 pif1Δ exo1Δ combinations; checkpoint activation assays; ssDNA accumulation assays in S. cerevisiae The EMBO journal Medium 21045806
2011 ScPif1 binds G4 motifs genome-wide in vivo; replication slows near G4-motif Pif1-binding sites and they are prone to breakage in Pif1-deficient cells; spontaneous mutations eliminating G4-forming ability relieve slow growth, DNA damage, and replication stalling in pif1Δ cells. Genome-wide ChIP; DNA replication assays; DNA breakage assays; spontaneous suppressor mutation analysis Cell High 21620135
2012 ScPif1 physically interacts with the mitochondrial single-stranded DNA binding protein Rim1; the OB-fold domain and C-terminal tail of Rim1 mediate the interaction; Rim1 stimulates Pif1 helicase activity 4–5-fold in vitro; this interaction likely plays a role in mtDNA metabolism. Proteomics (IDICT approach); in vitro co-precipitation with recombinant proteins; fluorescence anisotropy titration (Kd = 0.69 µM); deletion mutagenesis; helicase activity assay Nucleic acids research High 23175612
2013 G4-unwinding activity is conserved across Pif1 family helicases from organisms separated by >3 billion years of evolution; human PIF1 expressed in yeast suppresses both G4-associated DNA damage and telomere lengthening. In vitro G4 unwinding assays with Pif1 helicases from multiple species; expression of human PIF1 in yeast; DNA damage and telomere length assays Nature High 23657261
2013 ScPif1 monomers translocate on ssDNA with 5' to 3' directionality; monomers retain some unwinding activity, but dimers are more efficient helicases; Pif1 monomer translocation may displace proteins from ssDNA. Single-molecule fluorescence assays; ensemble biochemical assays with purified ScPif1 monomers Nucleic acids research Medium 23446274
2013 ScPif1 promotes DNA synthesis during break-induced replication (BIR) and crossover recombination by stimulating Pol δ-mediated DNA synthesis from D-loops; Pif1 liberates the newly synthesized strand to establish a migrating D-loop, preventing topological constraint accumulation and enabling extensive DNA synthesis. In vitro D-loop extension assay with purified Pif1, Pol δ, and Rad51; BIR genetic assay in S. cerevisiae; Pol δ recruitment assay Nature High 24025768
2013 ScPif1 unwinds dsDNA with a one-base-pair kinetic step size; unwinding rate ~75 bp/s; chemical efficiency ~0.84 ATP per nucleotide translocated; translocation rate on ssDNA equals unwinding rate, indicating Pif1 is an active helicase. Pre-steady-state kinetics; global fitting to stepwise unwinding model; streptavidin displacement assay; fluorescent phosphate-binding protein ATPase assay The Journal of biological chemistry High 23596008
2014 ScPif1 exhibits a DNA patrolling activity: a monomer anchors to a 3'-tailed DNA junction and periodically reels in the 3' tail with a 1-nucleotide step size, extruding a loop; this activity repeatedly unfolds G4 DNA one strand at a time (three steps) and is sufficient to unwind RNA-DNA heteroduplexes but not duplex DNA. Single-molecule fluorescence (smFRET, TIRF microscopy) with purified ScPif1 eLife High 24843019
2014 DNA damage-induced phosphorylation of ScPif1 by the Mec1-Rad53 checkpoint cascade is required for Pif1's role in break-induced replication and telomere elongation; this phosphorylation was previously known to inhibit telomerase at DSBs and is now shown also important for BIR. Genetic analysis of pif1 phosphorylation-site mutants in cdc9-1, cdc44-5, and rrm3Δ backgrounds; telomere length assays; BIR pathway epistasis PLoS genetics Medium 25329304
2015 ScPif1 binds more tightly to parallel G4 DNA than ssDNA or tailed duplexes, but unwinds parallel G4 more slowly than duplex DNA; ATP hydrolysis rate is reduced on parallel G4 compared to ssDNA; Pif1 unfolds parallel G4 repetitively in a multi-turnover reaction. In vitro fluorescence-based helicase assay; ATPase assay; single-cycle and multi-turnover G4 unfolding assays The Journal of biological chemistry Medium 25589786
2015 ScPif1 unfolds G4 in a sequential two-step mechanism, with G-triplex (G3) as a stable intermediate; Pif1 repetitively unfolds single G4 structures—after unfolding and stalling at the ssDNA/dsDNA junction, G4 rapidly reforms and Pif1 re-initiates unfolding. Single-molecule fluorescence assay (FRET) with purified ScPif1 and G4-containing DNA constructs mimicking lagging strand synthesis The Biochemical journal Medium 25471447
2015 G4 structures stimulate ScPif1-catalyzed duplex DNA unwinding through G4-enhanced Pif1 dimerization, which is required for duplex DNA unwinding. In vitro helicase assay; ATPase assay; dimerization analysis with recombinant nuclear ScPif1 The Journal of biological chemistry Medium 25627683
2015 ScPif1 acts preferentially at long telomeres to remove telomerase: Pif1-associated telomeres are ~70 bp longer than bulk telomeres; in pif1 mutants, both the fraction of lengthened telomeres and telomere extension per event increase; Pif1 preferentially binds long over short telomeres. Single telomere extension assay (STEX); ChIP of Pif1 and telomerase subunits (Est1, Est2); inducible short telomere assay PLoS genetics High 25906395
2015 Rad53 kinase phosphorylates Rrm3 and Pif1 helicases during replication stress; ablation of Rrm3 and Pif1 rescues rad53 mutant lethality, chromosome fragmentation, replisome-fork dissociation, fork reversal, and processing; phospho-mimicking rrm3 mutants ameliorate rad53 phenotypes. Genetic suppression; phospho-mimicking mutations; DNA fiber analysis; 2D gel electrophoresis; electron microscopy of replication intermediates Cell reports Medium 26411679
2016 ScPif1 removes Rap1 protein bound to its consensus site in front of Pol δ, stimulating strand displacement synthesis; Pif1 allows Pol δ to synthesize across arrays of Rap1 molecules mimicking a telomeric DNA-protein assembly. In vitro reconstituted strand displacement assay with purified Pif1, Pol δ, and Rap1; telomeric array substrates Nucleic acids research High 27001517
2016 ScPif1 is essential for efficient replication through lagging strand G4 sequences in vivo (measured by live-cell single-replication-fork imaging); Pif1 is dispensable for replication through the same G4s on the leading strand; a canonical PIP sequence in Pif1 interacts with PCNA and is required for optimal replisome progression through G4 sequences. Live-cell imaging of single replication forks in yeast; PIP-box mutant analysis; in vitro replication assays Nucleic acids research High 30395308
2016 Crystal structure of bacterial Bacteroides sp Pif1 (BaPif1) helicase domain in complex with ADP-AlF4- and ssDNA reveals: a wedge region (extended loop + α helix) acts as strand separator; the Pif1 signature motif stabilizes ssDNA-binding elements; domain 2B undergoes large conformational change upon ATP+ssDNA binding; ssDNA is bent ~90° at the ssDNA/dsDNA junction. X-ray crystallography of BaPif1 in multiple states; mutational analysis; helicase activity assays Cell reports High 26904952
2016 ScPif1 unwinds RNA:DNA heteroduplexes with moderately greater processivity than DNA:DNA duplexes due to slower dissociation from RNA:DNA hybrids, not due to differences in binding affinity or strand separation rate. In vitro helicase assays; kinetic analysis of unwinding; processivity measurements; dissociation rate measurements The Journal of biological chemistry Medium 26733194
2016 A ScPif1 monomer can unwind dsDNA; helicase activity of the monomer is modulated by a second DNA binding site that interacts with the 3'-ssDNA tail, which can promote re-winding activity; in excess Pif1, binding to this second site is precluded and re-winding is enhanced, masking monomer unwinding. DNA polymerase-coupled unwinding assay; FRET-based helicase assay with purified recombinant ScPif1 Journal of molecular biology Medium 26908222
2017 Crystal structure of ScPif1 in complex with PCNA reveals a non-canonical PCNA-interacting motif; a Pif1 mutant deficient in PCNA interaction impairs Pol δ-mediated DNA strand displacement synthesis in vitro and BIR efficiency in cells. X-ray crystallography; mutant Pif1-PCNA interaction analysis; in vitro strand displacement assay; BIR assay in S. cerevisiae Cell reports High 29141206
2017 ScPif1 promotes replication and suppresses DNA damage at tRNA genes (tDNAs); Pif1 binds multiple tDNAs in vivo; DNA damage at tDNAs in pif1Δ rrm3Δ cells is suppressed by destabilizing R-loops; Pif1 and Rrm3 binding to tDNAs increases upon R-loop stabilization. ChIP-seq; replication intermediate analysis by 2D gel; R-loop manipulation by RNase H overexpression; genetic analysis Nature communications High 28429714
2017 ScPif1 contributes to template switching (TS) DNA damage bypass by expanding single-stranded DNA gaps left behind replication forks; this function requires interaction with PCNA (replication clamp) for recruitment to damage sites and complements Exo1 in processing post-replicative daughter-strand gaps. Genetic epistasis with pif1, exo1, and PCNA interaction mutants; ssDNA gap analysis; damage sensitivity assays in S. cerevisiae Nucleic acids research Medium 30107417
2018 Human PIF1 helicase promotes break-induced replication (BIR) in mammalian cells; PCNA-dependent loading of PIF1 onto collapsed/broken forks is critical for BIR activation; the breast cancer-associated PIF1 mutant L319P is defective in BIR. EGFP-based BIR reporter assay; siRNA knockdown; PCNA interaction mutant analysis; AT-rich CFS-derived sequences for replication stress-induced BIR The EMBO journal High 33470420
2018 ScPif1 helicase is regulated by lysine acetylation: NuA4 acetyltransferase and Rpd3 deacetylase dynamically acetylate nuclear Pif1 at multiple sites; acetylation stimulates Pif1 helicase, ATPase, and DNA-binding activities and induces a conformational change. Pif1 overexpression toxicity assays; mass spectrometry identification of acetylation sites; biochemical helicase/ATPase/binding assays with acetylated Pif1; limited proteolysis The Journal of biological chemistry Medium 32878983
2018 The Pif1 signature motif (SM) is essential for ATPase activity but not substrate binding in ScPif1; the SM is required for all tested in vivo functions (mtDNA maintenance, telomerase inhibition at telomeres and DSBs, Okazaki fragment maturation); the SM must have the correct sequence from ScPif1, not a bacterial homolog. SM deletion and point mutation analysis; in vitro ATPase and binding assays; in vivo complementation for mtDNA maintenance, telomere length, OFM Nucleic acids research High 30239884
2018 The Pif1 signature motif of S. pombe Pfh1 is required for helicase unwinding and protein displacement activities but is dispensable for strand annealing and G4 binding; the disease/inviability-linked mutation L430P (equivalent to human L319P) abolishes unwinding and protein displacement but retains G4 binding and strand annealing. In vitro helicase assay; protein displacement assay; strand annealing assay; G4 binding assay with SM deletion and point mutants of nPfh1 Nucleic acids research High 30053106
2018 Dna2 processes long ssDNA flaps generated behind replication forks by Pif1 and replication-dependent strand displacement; electron microscopy shows accumulation of long ssDNA flaps behind forks in Dna2-depleted cells; PIF1 deletion rescues Dna2 depletion lethality. Conditional Dna2 depletion; electron microscopy of replication intermediates; genetic epistasis with pif1Δ, pol32Δ, rad9Δ Nature communications High 30446656
2019 Crystal structures of human PIF1 (hPIF1) in apo, ground-state and transition-state configurations reveal a conserved ssDNA binding channel critical for ssDNA binding during unwinding (but not G4 binding); the ssDNA channel is important for helicase activity but not strand annealing; significant structural divergence from bacterial and yeast Pif1 in the DNA strand separation wedge region. X-ray crystallography; mutational analysis of ssDNA channel residues; helicase, G4 binding, and strand annealing assays Nucleic acids research High 30698796
2019 Two BaPif1 molecules coordinate to unwind forked dsDNA: two interacting BaPif1 molecules bind each fork, one to the 5' arm and one to the 3' ss/dsDNA junction; the 5' arm binding causes a sharp bend breaking the first base pair; the 3'-bound molecule stabilizes the unpaired first base pair and engages the second for breaking. X-ray crystallography of BaPif1 in complex with symmetrical double forked dsDNA Nature communications High 31772234
2019 ScPif1 and Pfh1 unwind dsDNA by a branched mechanism with two modes: (1) dominant repetitive mode—short stretches of DNA are processively and repetitively opened without strand switching; (2) less frequent long-unwinding mode. The branching point is controlled by conformational selection based on helicase interaction with the 3' non-translocating strand. Single-molecule FRET with purified ScPif1 and Pfh1 Proceedings of the National Academy of Sciences of the United States of America High 31744872
2019 Pif1 helicase is required for fork convergence during DNA replication termination in eukaryotes: reconstituted yeast replication forks including Pif1 and Rrm3 promote efficient fork convergence and completion of DNA synthesis even without type II topoisomerase; Rrm3 and Pif1 are also important for plasmid DNA replication termination in vivo. In vitro reconstituted yeast replication fork system with purified proteins; plasmid DNA replication termination assay in vivo Molecular cell High 30850330
2019 Pif1 and SSBs (RPA/mtSSB) play complementary roles in promoting replication through G4 barriers: SSBs alone are effective for weak G4s but above a stability threshold, Pif1 helicase is required; head-on collision between Pif1 (moving 5'-3' on template) and polymerase δ or γ stimulates their 3'-exonuclease activity; RPA and mtSSB protect against excessive DNA degradation during this conflict. In vitro primer extension assays with a G4-stability series; RPA/SSB protection assays; polymerase exonuclease activity assays Nucleic acids research Medium 31340040
2020 Pif1 enables the replisome to bypass an R-loop/protein barrier: in a reconstituted S. cerevisiae replisome, Pif1 enables bypass of a dCas9 R-loop targeted to either strand; single-molecule imaging shows Pif1 simultaneously removes the dCas9 protein and the R-loop. In vitro reconstituted replisome with purified proteins; single-molecule fluorescence visualization; dCas9 R-loop barrier assay Proceedings of the National Academy of Sciences of the United States of America High 33199603
2020 RPA co-precipitates with Pif1 in S. cerevisiae; RPA and Pif1 cooperate in maintaining G4-containing CEB1 minisatellite stability on the leading strand (same pathway); the rfa1-D228Y mutation (reduced G-rich ssDNA affinity) reduces Pif1 association with CEB1. Co-precipitation assay; genetic instability assay; ChIP; strand-specific CEB1 minisatellite assay Cell stress Medium 32190820
2020 Pif1, RPA, and FEN1 modulate Pol δ strand displacement through protein barriers: Pif1 enables Pol δ to synthesize through Reb1, Tbf1, or nucleosome barriers; FEN1 prevents unwarranted Pif1-dependent re-replication by cleaving 5' tails that would serve as Pif1 entry points during lagging strand synthesis. In vitro reconstituted strand displacement assay with purified proteins, positioned nucleosomes, and transcription factor barriers The Journal of biological chemistry High 32913126
2021 Human PIF1 helicase promotes BIR specifically for long-track DNA synthesis but not short-track gene conversion; L319P breast cancer-associated mutant is defective in BIR; synthetic lethality between PIF1 and FANCM loss. EGFP-based recombination reporters for BIR vs. STGC; siRNA knockdown; PIF1 mutant (L319P) functional analysis; synthetic lethality assay The EMBO journal High 33470420
2021 Pif1 is actively inhibited during meiotic recombination by the Mer3-MutLβ complex, which competes with Pif1 for binding to RFC-PCNA; Pif1 (via its PCNA interaction) is required for long gene conversion tracts observed in the absence of MutLβ; in vitro, Mer3-MutLβ inhibits Pif1-stimulated D-loop extension by Pol δ and RFC-PCNA. In vitro D-loop extension assay; Co-IP of Mer3 with RFC; ChIP-seq of Pif1 at meiotic DSB sites; genetic analysis of PCNA-interaction mutants Nucleic acids research High 33823531
2022 X-ray crystal structure of Thermus oshimai Pif1 (ToPif1) complexed with a G4 reveals a G4-Recognizing Surface (GRS) at domains 1B/2B that recognizes the entire G4 via electrostatic, ionic interactions, and hydrogen bonds with the ribose-phosphate backbone; the G4 maintains its three-layered propeller topology without G-tetrad reorganization upon protein binding. X-ray crystallography of ToPif1-G4 complex EMBO reports High 35736675
2014 Human PIF1 depletion slows replication fork rates and increases stalled forks during normal cycling conditions; PIF1-dependent replication impediments impair S-phase progression in RAS oncogene-transformed fibroblasts but not parental cells; CHK1 activation is suppressed in PIF1-depleted tumor cells released from S-phase arrest. siRNA knockdown in human cells; DNA fiber analysis; flow cytometry; CHK1 phosphorylation analysis Oncotarget Medium 25359767
2018 Human PIF1 has a role in DNA resection at G4-prone sequences: PIF1 is recruited to DNA damage sites; PIF1 physically interacts with proteins involved in DNA resection; PIF1 depletion reduces HR efficiency and causes DNA damage sensitivity; G4 stabilization hampers DNA resection in a manner suppressed by PIF1 overexpression. siRNA knockdown; Co-IP of PIF1 with resection factors; HR reporter assay; G4 ligand treatment; recruitment to DSB by ChIP Cell reports Medium 30232007
2018 ScPif1 unfolds telomeric G4 (antiparallel/mixed hybrid) more readily than parallel c-MYC promoter G4; under low-stability G4 conditions (Na+), Pif1 traps thermally melted G4 without ATP; stable telomeric G4 is unfolded stepwise with slower rate and slower Pif1 dissociation than from duplexes. In vitro fluorescence-based G4 unfolding assay; ATPase assay; single-cycle and multi-turnover conditions; various monovalent cation conditions The Journal of biological chemistry Medium 30257865
2014 Human PIF1 depletion triggers apoptosis in tumor cells (both p53-deficient and p53-proficient) but not in non-malignant cells; death occurs in late G1/early S-phase and depends on caspase-3; CHK1 activation is suppressed upon PIF1 depletion; PIF1 is required for S-phase checkpoint signaling. siRNA knockdown in tumor vs. non-malignant human cells; flow cytometry; caspase-3 activity assay; CHK1 phosphorylation analysis Cancer research Medium 21616935
2018 ScPif1 and Hrq1 (yeast RECQL4 homolog) synergistically inhibit or stimulate telomerase activity depending on which helicase is catalytically active; the two helicases interact functionally, suggesting coordinated roles in telomere length homeostasis. In vitro telomerase primer extension assay with yeast extracts enriched for telomerase; titrations of recombinant WT and catalytically inactive Hrq1 and Pif1; combined helicase reactions The Journal of biological chemistry Medium 30068549
2019 Drosophila melanogaster PIF1 promotes processive DNA synthesis during double-strand gap repair specifically in the absence of POL32; loss of PIF1 combined with loss of BRCA2 is synthetically lethal; PIF1 functions with POL32 during replication stress. pif1 null mutant Drosophila; gap repair synthesis assay; synthetic lethality with brca2; hydroxyurea sensitivity assay Genetics Medium 31537623
2020 PIF1 interacts with TERT (telomerase reverse transcriptase) in cervical cancer cells as demonstrated by co-immunoprecipitation; PIF1 knockdown down-regulates telomerase activity and reduces proliferation, while promoting apoptosis. Co-immunoprecipitation; siRNA knockdown; ELISA-based telomerase activity assay; flow cytometry Cancer management and research Low 32943924

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2011 DNA replication through G-quadruplex motifs is promoted by the Saccharomyces cerevisiae Pif1 DNA helicase. Cell 481 21620135
2013 Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature 402 23657261
1994 The saccharomyces PIF1 DNA helicase inhibits telomere elongation and de novo telomere formation. Cell 324 8287473
2009 The yeast Pif1 helicase prevents genomic instability caused by G-quadruplex-forming CEB1 sequences in vivo. PLoS genetics 302 19424434
2013 Pif1 helicase and Polδ promote recombination-coupled DNA synthesis via bubble migration. Nature 273 24025768
2010 Unwinding the functions of the Pif1 family helicases. DNA repair 180 20097624
2006 Evidence suggesting that Pif1 helicase functions in DNA replication with the Dna2 helicase/nuclease and DNA polymerase delta. Molecular and cellular biology 179 16537895
1991 PIF1: a DNA helicase in yeast mitochondria. The EMBO journal 164 1849081
2014 Periodic DNA patrolling underlies diverse functions of Pif1 on R-loops and G-rich DNA. eLife 142 24843019
2017 A PIF1/PIF3-HY5-BBX23 Transcription Factor Cascade Affects Photomorphogenesis. Plant physiology 138 28687557
2010 Human Pif1 helicase is a G-quadruplex DNA-binding protein with G-quadruplex DNA-unwinding activity. The Biochemical journal 129 20524933
2006 Roles of Pif1-like helicases in the maintenance of genomic stability. Nucleic acids research 111 16935874
2017 PIF1 family DNA helicases suppress R-loop mediated genome instability at tRNA genes. Nature communications 106 28429714
2015 CUL4 forms an E3 ligase with COP1 and SPA to promote light-induced degradation of PIF1. Nature communications 102 26037329
2009 Pif1 helicase lengthens some Okazaki fragment flaps necessitating Dna2 nuclease/helicase action in the two-nuclease processing pathway. The Journal of biological chemistry 90 19605347
2008 Pif1 helicase directs eukaryotic Okazaki fragments toward the two-nuclease cleavage pathway for primer removal. The Journal of biological chemistry 88 18689797
2017 Identification of HDA15-PIF1 as a key repression module directing the transcriptional network of seed germination in the dark. Nucleic acids research 85 28444370
1993 PIF1 DNA helicase from Saccharomyces cerevisiae. Biochemical characterization of the enzyme. The Journal of biological chemistry 85 8253734
2021 PIF1 helicase promotes break-induced replication in mammalian cells. The EMBO journal 84 33470420
2006 The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity. Nucleic acids research 81 16522649
2018 Pif1 is essential for efficient replisome progression through lagging strand G-quadruplex DNA secondary structures. Nucleic acids research 75 30395308
2019 Pif1-Family Helicases Support Fork Convergence during DNA Replication Termination in Eukaryotes. Molecular cell 73 30850330
2016 PIF1-Interacting Transcription Factors and Their Binding Sequence Elements Determine the in Vivo Targeting Sites of PIF1. The Plant cell 72 27303023
2017 Role of the Pif1-PCNA Complex in Pol δ-Dependent Strand Displacement DNA Synthesis and Break-Induced Replication. Cell reports 70 29141206
2015 Molecular mechanism of G-quadruplex unwinding helicase: sequential and repetitive unfolding of G-quadruplex by Pif1 helicase. The Biochemical journal 67 25471447
2016 Pif1-family helicases cooperatively suppress widespread replication-fork arrest at tRNA genes. Nature structural & molecular biology 66 27991904
2010 Pif1- and Exo1-dependent nucleases coordinate checkpoint activation following telomere uncapping. The EMBO journal 65 21045806
2012 A genomewide screen for suppressors of Alu-mediated rearrangements reveals a role for PIF1. PloS one 63 22347400
2022 CircNEIL3 mediates pyroptosis to influence lung adenocarcinoma radiotherapy by upregulating PIF1 through miR-1184 inhibition. Cell death & disease 60 35190532
2017 Structure and function of Pif1 helicase. Biochemical Society transactions 59 28900015
2015 A parallel quadruplex DNA is bound tightly but unfolded slowly by pif1 helicase. The Journal of biological chemistry 59 25589786
2006 Murine Pif1 interacts with telomerase and is dispensable for telomere function in vivo. Molecular and cellular biology 59 17130244
2007 Mitochondrial and nuclear localization of human Pif1 helicase. Biological & pharmaceutical bulletin 57 17827721
2011 The Pif1 family in prokaryotes: what are our helicases doing in your bacteria? Molecular biology of the cell 53 21670310
2009 Human Pif1 helicase unwinds synthetic DNA structures resembling stalled DNA replication forks. Nucleic acids research 53 19700773
2021 The PIF1-miR408-PLANTACYANIN repression cascade regulates light-dependent seed germination. The Plant cell 52 33616669
2015 Rad53-Mediated Regulation of Rrm3 and Pif1 DNA Helicases Contributes to Prevention of Aberrant Fork Transitions under Replication Stress. Cell reports 50 26411679
2013 Yeast Pif1 helicase exhibits a one-base-pair stepping mechanism for unwinding duplex DNA. The Journal of biological chemistry 50 23596008
2002 Schizosaccharomyces pombe pfh1+ encodes an essential 5' to 3' DNA helicase that is a member of the PIF1 subfamily of DNA helicases. Molecular biology of the cell 47 12058079
2019 Complementary roles of Pif1 helicase and single stranded DNA binding proteins in stimulating DNA replication through G-quadruplexes. Nucleic acids research 45 31340040
2016 Getting it done at the ends: Pif1 family DNA helicases and telomeres. DNA repair 43 27233114
2013 Translocation of Saccharomyces cerevisiae Pif1 helicase monomers on single-stranded DNA. Nucleic acids research 42 23446274
2010 DNA binding induces dimerization of Saccharomyces cerevisiae Pif1. Biochemistry 42 20795654
2020 Replisome bypass of a protein-based R-loop block by Pif1. Proceedings of the National Academy of Sciences of the United States of America 41 33199603
2015 G-quadruplexes significantly stimulate Pif1 helicase-catalyzed duplex DNA unwinding. The Journal of biological chemistry 41 25627683
2010 An alternative pathway for Okazaki fragment processing: resolution of fold-back flaps by Pif1 helicase. The Journal of biological chemistry 41 20959454
2006 The absence of Top3 reveals an interaction between the Sgs1 and Pif1 DNA helicases in Saccharomyces cerevisiae. Genetics 40 16816432
2020 Yeast Genome Maintenance by the Multifunctional PIF1 DNA Helicase Family. Genes 39 32093266
2016 Structural and Functional Insights into the Unwinding Mechanism of Bacteroides sp Pif1. Cell reports 39 26904952
2012 Physical and functional interaction between yeast Pif1 helicase and Rim1 single-stranded DNA binding protein. Nucleic acids research 39 23175612
2016 Yeast Helicase Pif1 Unwinds RNA:DNA Hybrids with Higher Processivity than DNA:DNA Duplexes. The Journal of biological chemistry 38 26733194
2018 Pif1 helicase unfolding of G-quadruplex DNA is highly dependent on sequence and reaction conditions. The Journal of biological chemistry 36 30257865
2015 The Bacteroides sp. 3_1_23 Pif1 protein is a multifunctional helicase. Nucleic acids research 36 26384418
2014 Human PIF1 helicase supports DNA replication and cell growth under oncogenic-stress. Oncotarget 35 25359767
2018 The Helicase PIF1 Facilitates Resection over Sequences Prone to Forming G4 Structures. Cell reports 34 30232007
2016 Pif1 removes a Rap1-dependent barrier to the strand displacement activity of DNA polymerase δ. Nucleic acids research 34 27001517
2011 Suppression of apoptosis by PIF1 helicase in human tumor cells. Cancer research 34 21616935
2019 Structural and functional analysis of the nucleotide and DNA binding activities of the human PIF1 helicase. Nucleic acids research 33 30698796
2014 Break-induced replication requires DNA damage-induced phosphorylation of Pif1 and leads to telomere lengthening. PLoS genetics 33 25329304
2015 The pif1 helicase, a negative regulator of telomerase, acts preferentially at long telomeres. PLoS genetics 32 25906395
2018 Dna2 processes behind the fork long ssDNA flaps generated by Pif1 and replication-dependent strand displacement. Nature communications 31 30446656
2016 Pif1 is a force-regulated helicase. Nucleic acids research 31 27098034
2008 Biochemical analysis of human PIF1 helicase and functions of its N-terminal domain. Nucleic acids research 31 18835853
2018 The WYL Domain of the PIF1 Helicase from the Thermophilic Bacterium Thermotoga elfii is an Accessory Single-Stranded DNA Binding Module. Biochemistry 30 29341597
2014 The Pif1 family helicase Pfh1 facilitates telomere replication and has an RPA-dependent role during telomere lengthening. DNA repair 29 25303777
2018 DNA-unwinding activity of Saccharomyces cerevisiae Pif1 is modulated by thermal stability, folding conformation, and loop lengths of G-quadruplex DNA. The Journal of biological chemistry 28 30305390
2019 Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases. Nature communications 27 31772234
2018 The helicase Pif1 functions in the template switching pathway of DNA damage bypass. Nucleic acids research 27 30107417
2014 To peep into Pif1 helicase: multifaceted all the way from genome stability to repair-associated DNA synthesis. Journal of microbiology (Seoul, Korea) 26 24500472
2019 Pif1 family DNA helicases: A helpmate to RNase H? DNA repair 24 31231063
2009 Telomerase is essential to alleviate pif1-induced replication stress at telomeres. Genetics 24 19704012
2020 RPA and Pif1 cooperate to remove G-rich structures at both leading and lagging strand. Cell stress 23 32190820
2018 The Saccharomyces cerevisiae Hrq1 and Pif1 DNA helicases synergistically modulate telomerase activity in vitro. The Journal of biological chemistry 23 30068549
2014 A histone methyltransferase inhibits seed germination by increasing PIF1 mRNA expression in imbibed seeds. The Plant journal : for cell and molecular biology 23 24635727
2014 Pif1 regulates telomere length by preferentially removing telomerase from long telomere ends. Nucleic acids research 23 24981509
2021 The Pif1 helicase is actively inhibited during meiotic recombination which restrains gene conversion tract length. Nucleic acids research 22 33823531
2017 The functions of the multi-tasking Pfh1Pif1 helicase. Current genetics 22 28054200
2017 A sharp Pif1-dependent threshold separates DNA double-strand breaks from critically short telomeres. eLife 22 28826474
2014 Yeast Pif1 accelerates annealing of complementary DNA strands. Biochemistry 22 25393406
2020 PCH1 and PCHL Directly Interact with PIF1, Promote Its Degradation, and Inhibit Its Transcriptional Function during Photomorphogenesis. Molecular plant 21 32061894
2020 PIF1 Affects the Proliferation and Apoptosis of Cervical Cancer Cells by Influencing TERT. Cancer management and research 21 32943924
2018 The Pif1 signature motif of Pfh1 is necessary for both protein displacement and helicase unwinding activities, but is dispensable for strand-annealing activity. Nucleic acids research 21 30053106
2018 Two Pif1 Family DNA Helicases Cooperate in Centromere Replication and Segregation in Saccharomyces cerevisiae. Genetics 21 30442759
2016 A Monomer of Pif1 Unwinds Double-Stranded DNA and It Is Regulated by the Nature of the Non-Translocating Strand at the 3'-End. Journal of molecular biology 21 26908222
2009 Mixtures of complete and pif1- and pif2-deficient genotypes are required for increased potency of an insect nucleopolyhedrovirus. Journal of virology 21 19264787
2020 Lysine acetylation regulates the activity of nuclear Pif1. The Journal of biological chemistry 20 32878983
2021 Structural and functional studies of SF1B Pif1 from Thermus oshimai reveal dimerization-induced helicase inhibition. Nucleic acids research 19 33784404
2011 Multiple kinases promote light-induced degradation of PIF1. Plant signaling & behavior 19 21758014
2019 The Biochemical Activities of the Saccharomyces cerevisiae Pif1 Helicase Are Regulated by Its N-Terminal Domain. Genes 18 31142053
2019 The Drosophila melanogaster PIF1 Helicase Promotes Survival During Replication Stress and Processive DNA Synthesis During Double-Strand Gap Repair. Genetics 18 31537623
2019 Branched unwinding mechanism of the Pif1 family of DNA helicases. Proceedings of the National Academy of Sciences of the United States of America 18 31744872
2020 Downregulation of PIF1, a potential new target of MYCN, induces apoptosis and inhibits cell migration in neuroblastoma cells. Life sciences 17 32512012
2020 Pif1, RPA, and FEN1 modulate the ability of DNA polymerase δ to overcome protein barriers during DNA synthesis. The Journal of biological chemistry 17 32913126
2022 Structural mechanism underpinning Thermus oshimai Pif1-mediated G-quadruplex unfolding. EMBO reports 16 35736675
2012 TbPIF8, a Trypanosoma brucei protein related to the yeast Pif1 helicase, is essential for cell viability and mitochondrial genome maintenance. Molecular microbiology 16 22220754
2017 Nitric oxide promotes light-initiated seed germination by repressing PIF1 expression and stabilizing HFR1. Plant physiology and biochemistry : PPB 15 29248678
2024 The phytochrome-interacting factor genes PIF1 and PIF4 are functionally diversified due to divergence of promoters and proteins. The Plant cell 14 38593049
2022 Rad51-mediated interhomolog recombination during budding yeast meiosis is promoted by the meiotic recombination checkpoint and the conserved Pif1 helicase. PLoS genetics 13 36508468
2018 The signature motif of the Saccharomyces cerevisiae Pif1 DNA helicase is essential in vivo for mitochondrial and nuclear functions and in vitro for ATPase activity. Nucleic acids research 13 30239884
2017 Telomerase regulation by the Pif1 helicase: a length-dependent effect? Current genetics 13 29052759

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