Affinage

Showing PHB1 — PHB is a alias.

PHB1

Prohibitin 1 · UniProt P35232

Length
272 aa
Mass
29.8 kDa
Annotated
2026-06-10
100 papers in source corpus 20 papers cited in narrative 20 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PHB1 (prohibitin 1) is a multifunctional scaffold protein best characterized as a mitochondrial inner-membrane chaperone that, with PHB2, assembles into a large multimeric ring complex (~12–14 copies of each subunit) which binds newly synthesized mitochondrial translation products and protects them from membrane-bound AAA proteases (PMID:11852914). Through this scaffold the PHB complex stabilizes respiratory supercomplex assembly without changing individual complex subunit abundance, limiting basal ROS production and preserving mitochondrial membrane potential (PMID:18086671, PMID:28630166). The complex is itself stabilized in the inner membrane through associations with SLP2/STOML2 and CHCHD10, and its destabilization disrupts cristae organization (MICOS) and triggers the OMA1–OPA1 fragmentation cascade (PMID:35656794, PMID:34781982). Beyond bioenergetics, PHB1 supports mitochondrial quality control: in yeast orthologs the prohibitin subunits act as Atg8/LC3 receptors driving mitophagy via AIM/LIR motifs and restrain Atg32 processing (PMID:38964378), and in mammalian cells PHB1 promotes mitophagy to clear damaged mtDNA and thereby limit NLRP3 inflammasome activation (PMID:37359543). PHB1 also operates outside mitochondria, forming a nuclear complex with the histone H3.3 chaperone HIRA to control H3.3 deposition, isocitrate dehydrogenase gene transcription, and α-ketoglutarate production required for ESC self-renewal (PMID:27939217), and regulating meiotic STAG3 expression through a JAK2/STAT-dependent histone modification pathway essential for male fertility (PMID:32232334). PHB1 abundance is set by TRIM21-mediated ubiquitination and degradation, which is competitively blocked by LPLUNC1 or ERβ (PMID:30886235, PMID:35599350), and its activity is modulated by AKT-mediated phosphorylation that controls its mitochondrial localization (PMID:29415747, PMID:32859869). In cancer contexts PHB1 scaffolds C-RAF/STOML2-driven ERK/MAPK signaling, stabilizes β-catenin to drive Wnt signaling and EMT, and associates with the eIF4F initiation complex to promote oncogene (MYC) translation (PMID:31334335, PMID:34781982, PMID:37235908, PMID:37084385).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 2002 High

    Established the founding molecular function of PHB1: that it and PHB2 form a ring-shaped membrane-bound chaperone protecting nascent mitochondrial translation products from proteolysis.

    Evidence Biochemical fractionation, native molecular weight analysis, and direct binding to mitochondrial translation products

    PMID:11852914

    Open questions at the time
    • Structural basis of the ring assembly not resolved
    • Did not address non-mitochondrial functions
  2. 2007 High

    Resolved where PHB1 acts in human cells and that it is post-translationally modified, linking the phosphorylated PHB1/PHB2 complex to membrane potential and cell survival.

    Evidence Subcellular fractionation, EM, MS phosphosite mapping, mutagenesis, and siRNA knockdown in human T cells

    PMID:18086671

    Open questions at the time
    • Kinase responsible for PHB1 serine phosphorylation not identified
    • Functional role of specific PHB1 phosphosites unresolved
  3. 2016 High

    Defined a distinct nuclear function for PHB1 as a HIRA partner coupling histone H3.3 deposition to metabolic gene expression and stem cell self-renewal.

    Evidence Genome-wide siRNA screen, reciprocal co-IP, chromatin assays, and metabolite measurement in human ESCs

    PMID:27939217

    Open questions at the time
    • How PHB1 partitions between mitochondria and nucleus unknown
    • Direct DNA/chromatin contact by PHB1 not shown
  4. 2017 High

    Showed the functional consequence of PHB chaperone activity is respiratory supercomplex assembly, distinguishing assembly from subunit abundance.

    Evidence siRNA knockdown, blue native PAGE, mitoflash imaging, ROS measurement, and co-expression rescue

    PMID:28630166

    Open questions at the time
    • Direct contacts between PHB ring and supercomplex subunits not mapped
  5. 2020 High

    Demonstrated that PHB1 controls meiotic progression in vivo by regulating cohesin (STAG3) through a non-canonical JAK2/STAT–histone modification axis.

    Evidence Conditional knockout mouse, meiotic staging, ChIP for H3Y41ph/H3K9me3 at the Stag3 locus, pathway Western blots

    PMID:32232334

    Open questions at the time
    • Whether PHB1 directly engages JAK2 vs. acts indirectly unclear
    • Relative contribution of mitochondrial vs. epigenetic roles to infertility not separated
  6. 2022 High

    Placed the PHB complex within the inner-membrane organizing network, showing its stability depends on SLP2/CHCHD10 and its loss collapses cristae via OMA1–OPA1 and MICOS disruption.

    Evidence Patient fibroblasts and CHCHD10(S59L) mouse model with reciprocal co-IP and morphology analysis

    PMID:35656794

    Open questions at the time
    • Direct PHB1–OPA1/mitofilin contacts inferred rather than proven
    • Stoichiometry of SLP2–PHB assembly not defined
  7. 2024 High

    Identified prohibitins as direct Atg8/LC3 mitophagy receptors via AIM/LIR motifs and as negative regulators of Atg32 processing, defining a quality-control function.

    Evidence Yeast deletion mutants, co-IP with Atg8, AIM/LIR mutagenesis, and genetic epistasis with Yme1/Pcp1

    PMID:38964378

    Open questions at the time
    • Demonstrated in yeast orthologs; mammalian PHB1 LIR-dependent receptor role not directly tested here
  8. 2023 Medium

    Linked PHB1-driven mitophagy to innate immune control by showing it limits cytoplasmic mtDNA accumulation and NLRP3 inflammasome activation.

    Evidence siRNA knockdown, cytoplasmic mtDNA quantification, NLRP3 activation assays, and mitophagy inhibitor epistasis

    PMID:37359543

    Open questions at the time
    • Single loss-of-function study
    • Whether PHB1 acts as the receptor in this mammalian mitophagy not shown directly
  9. 2019 Medium

    Established TRIM21 as the E3 ligase setting PHB1 abundance and LPLUNC1 as a competitive stabilizer, defining how PHB1 levels are controlled.

    Evidence Co-IP, ubiquitination and competitive binding assays, knockdown/overexpression, and NF-κB reporters in nasopharyngeal carcinoma

    PMID:30886235

    Open questions at the time
    • TRIM21 ubiquitination site on PHB1 not mapped
    • Single lab
  10. 2022 Medium

    Extended the TRIM21 degradation axis by showing ERβ competitively protects PHB1, corroborating the regulated-degradation model in a second disease context.

    Evidence Colitis models, ERβ knockdown in mice, co-IP and ubiquitination assays

    PMID:35599350

    Open questions at the time
    • Direct ERβ–PHB1 binding interface not defined
    • Single lab
  11. 2018 Medium

    Showed AKT-mediated phosphorylation governs PHB1 mitochondrial localization and downstream proliferation control via GADD45α.

    Evidence Co-IP, phosphorylation assays, fractionation, microarray, and GADD45α knockdown epistasis in bladder carcinoma

    PMID:29415747

    Open questions at the time
    • AKT phosphosite on PHB1 not mapped in this study
    • Single lab
  12. 2020 Medium

    Mapped a specific AKT phosphosite (T258) on PHB1 in sperm and connected PI3K/AKT–PHB1 signaling to motility and human asthenospermia.

    Evidence Co-localization, co-IP, wortmannin inhibition, phosphospecific antibodies, and human sperm analysis

    PMID:32859869

    Open questions at the time
    • Functional readout correlative in human samples
    • Single lab
  13. 2021 Medium

    Identified STOML2 as a direct PHB1 partner that co-activates RAF1–MEK–ERK signaling to promote proliferation.

    Evidence Yeast two-hybrid, co-IP, co-localization, knockdown, and MAPK Western blots in colorectal cancer

    PMID:34781982

    Open questions at the time
    • Whether STOML2–PHB1 acts via mitochondrial or cytosolic pool unclear
    • Single lab
  14. 2023 Medium

    Defined two additional pro-oncogenic mechanisms: PHB1 stabilization of β-catenin driving Wnt/EMT, and direct PHB association with eIF4F to promote MYC translation.

    Evidence Co-IP, knockdown/overexpression, migration/metastasis assays (β-catenin); multiomics, knockdown, and FL3 phenocopy with ribosome profiling (eIF4F)

    PMID:37084385 PMID:37235908

    Open questions at the time
    • Direct vs. scaffold-mediated PHB1–eIF4F binding not fully resolved
    • RAS-RAF-MAPK explicitly not implicated in CLL translation control

Open questions

Synthesis pass · forward-looking unresolved questions
  • How PHB1 is partitioned among mitochondrial, nuclear, cytosolic and translational pools, and how a single ring chaperone is repurposed for chromatin, signaling and translation roles, remains unresolved.
  • No structural model linking the inner-membrane ring to extra-mitochondrial functions
  • Trafficking/targeting signals directing PHB1 to nucleus or eIF4F not identified
  • Phosphorylation/ubiquitination code coordinating the multiple pools not integrated

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0044183 protein folding chaperone 2 GO:0060089 molecular transducer activity 2 GO:0042393 histone binding 1 GO:0140096 catalytic activity, acting on a protein 1 GO:0140313 molecular sequestering activity 1
Localization
GO:0005739 mitochondrion 4 GO:0005634 nucleus 2
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-392499 Metabolism of proteins 2 R-HSA-4839726 Chromatin organization 2 R-HSA-9612973 Autophagy 2 R-HSA-168256 Immune System 1
Partners
AKTCHCHD10CRAFCTNNB1HIRAPHB2STOML2TRIM21
Complex memberships
PHB-HIRA complexPHB1/PHB2 ring complexSLP2-prohibitin complexeIF4F complex

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2002 PHB1 (Phb1p) and PHB2 (Phb2p) localize to the mitochondrial inner membrane where they form a large multimeric ring complex (~12-14 copies of each subunit) that acts as a membrane-bound chaperone, directly binding newly synthesized mitochondrial translation products and stabilizing them against degradation by membrane-bound AAA metalloproteases. Biochemical fractionation, native molecular weight analysis, direct binding assays with mitochondrial translation products Cellular and molecular life sciences : CMLS High 11852914
2007 In human T cells, PHB1 and PHB2 localize to the mitochondrial inner membrane (not the nucleus); PHB1 is serine-phosphorylated and PHB2 is serine- and tyrosine-phosphorylated (Tyr248 mapped by MS and confirmed by mutagenesis). siRNA-mediated knockdown of PHBs disrupts mitochondrial membrane potential, establishing a role for the PHB1/PHB2 phosphocomplex in mitochondrial homeostasis and T cell survival. Subcellular fractionation, immunofluorescence, electron microscopy, orthophosphate labeling, phosphoamino acid analysis, mass spectrometry, site-directed mutagenesis, phosphospecific antibodies, siRNA knockdown The Journal of biological chemistry High 18086671
2016 In human ESCs, PHB forms a protein complex with HIRA (a histone H3.3 chaperone), stabilizes HIRA complex components, and together with HIRA controls global deposition of histone H3.3 and gene expression. PHB and HIRA regulate chromatin architecture at isocitrate dehydrogenase gene promoters to promote transcription and α-ketoglutarate production. Genome-wide siRNA screening identified PHB as essential for hESC self-renewal. Genome-wide siRNA screen, co-immunoprecipitation, chromatin assays, gene expression analysis, metabolite measurement Cell stem cell High 27939217
2017 PHB complex deficiency impairs the formation of mitochondrial respiratory supercomplexes (RSCs) without altering the abundance of individual respiratory complex subunits, leading to elevated basal mitochondrial ROS production and increased mitoflash frequency. Co-expression of PHB1 and PHB2 rescues these defects, indicating the multimeric PHB complex is the functional unit for RSC assembly. siRNA knockdown, live-cell mitoflash imaging, ROS measurement, blue native PAGE for RSC analysis, rescue by co-expression Journal of cell science High 28630166
2019 TRIM21 acts as an E3 ubiquitin ligase that ubiquitinates PHB1, targeting it for degradation. LPLUNC1 stabilizes PHB1 by competitively impeding the PHB1-TRIM21 interaction (due to stronger binding affinity of LPLUNC1 to PHB1), thus inhibiting PHB1 ubiquitination. PHB1 suppresses NF-κB activity in nasopharyngeal carcinoma cells. Co-immunoprecipitation, ubiquitination assays, competitive binding assays, PHB1 knockdown/overexpression, NF-κB reporter assays Oncogene Medium 30886235
2013 PHB overexpression in undifferentiated rat granulosa cells inhibits apoptosis by increasing anti-apoptotic proteins Bcl-2 and Bcl-xL, reducing cytochrome c release from mitochondria, and inhibiting caspase-3 activity via a PHB→MEK-ERK1/2→Bcl-2/Bcl-xL pathway. PHB silencing fragmented mitochondrial morphology and sensitized cells to apoptosis. Microarray, immunoblot, adenoviral overexpression and siRNA knockdown, mitochondrial morphology imaging, caspase activity assay, cytochrome c release measurement Apoptosis : an international journal on programmed cell death Medium 24096434
2011 PHB overexpression in rat granulosa cells increases mitochondrial PHB content and delays ceramide-induced apoptosis, while adenoviral shRNA silencing of PHB sensitizes cells to ceramide-induced apoptosis. Exogenous ceramide augments mitochondrial PHB expression and causes cytochrome c release and caspase-3 activation, establishing mitochondrial PHB as a critical survival factor in granulosa cells. Adenoviral overexpression and shRNA knockdown, ceramide treatment, cytochrome c release assay, caspase-3 activation measurement Life sciences Medium 21763324
2019 FoxM1 binds the PHB1 promoter and enhances PHB1 expression at transcriptional and post-transcriptional levels. PHB1 interacts with C-RAF and promotes ERK1/2 phosphorylation, contributing to paclitaxel resistance. Knockdown of PHB1 sensitizes pancreatic cancer cells to paclitaxel, and FoxM1 inhibition decreases PHB1, p-ERK1/2, and ABCA2 expression. Promoter binding assay (bioinformatics + reporter), co-immunoprecipitation (PHB1/C-RAF), gene knockdown, phospho-ERK Western blot, in vitro and in vivo paclitaxel resistance assays Molecular therapy oncolytics Medium 31334335
2020 PHB in spermatocytes regulates expression of STAG3 (a meiotic cohesin complex component) via a non-canonical JAK2/STAT pathway. The PHB/JAK2 axis modulates histone H3 tyrosine 41 phosphorylation (H3Y41ph) and H3K9me3 at the Stag3 locus. Loss of PHB in male germ cells (Phb-cKO) causes meiotic pachytene arrest, impaired DSB repair, and complete male infertility, also associated with mitochondrial dysfunction. Conditional knockout mouse (Phb-cKO), meiotic staging, immunofluorescence, ChIP (H3Y41ph, H3K9me3 at Stag3 locus), Western blot for JAK2/STAT pathway components, STAG3 expression analysis Nucleic acids research High 32232334
2022 CHCHD10 interacts with SLP2 (Stomatin-Like Protein 2) and participates in stabilizing the prohibitin complex in the mitochondrial inner membrane. The ALS/FTD-linked CHCHD10(S59L) variant causes SLP2-prohibitin aggregates and instability of the PHB complex, leading to OMA1 cascade activation, OPA1 processing, mitochondrial fragmentation, abnormal cristae morphogenesis, and motor neuron death. PHB complex destabilization disrupts the mitochondrial contact site and cristae organizing system (MICOS) complex, likely via disruption of OPA1-mitofilin interaction. Patient fibroblasts, mouse model (Chchd10S59L/+), co-immunoprecipitation, immunofluorescence, spinal cord histology, mitochondrial morphology analysis Brain : a journal of neurology High 35656794
2018 FL3 (a flavagline) inhibits the interaction between Akt and PHB1 and inhibits Akt-mediated PHB1 phosphorylation, which decreases PHB1 localization to mitochondria. This activates the GADD45α pathway leading to G2/M cell cycle arrest in bladder carcinoma cells. Knockdown of PHB1 mimics FL3-induced cell cycle inhibition, and knockdown of GADD45α rescues the inhibitory effect of FL3. Co-immunoprecipitation (Akt-PHB interaction), phosphorylation assays, subcellular fractionation, mRNA microarray, PHB1 knockdown, GADD45α knockdown, in vitro and in vivo proliferation assays Journal of experimental & clinical cancer research : CR Medium 29415747
2021 STOML2 interacts with PHB (PHB1) as demonstrated by yeast two-hybrid screening and confirmed by co-immunoprecipitation and co-localization in cell lines and tissues. Both STOML2 and PHB activate the MAPK signaling pathway (RAF1-MEK1/2-ERK1/2 phosphorylation) to promote colorectal cancer proliferation. Yeast two-hybrid, co-immunoprecipitation, immunofluorescence co-localization, STOML2 knockdown, MAPK pathway Western blot Journal of experimental & clinical cancer research : CR Medium 34781982
2023 PHB1 knockdown increases cytoplasmic mtDNA levels and enhances NLRP3 inflammasome activation. Mitophagy inhibitor treatment abolishes PHB1 knockdown-mediated NLRP3 activation, establishing that PHB1 inhibits NLRP3 inflammasome activation through promoting mitophagy. PHB1 siRNA knockdown, mtDNA cytoplasmic measurement, NLRP3 inflammasome activation assays, mitophagy inhibitor treatment Frontiers in immunology Medium 37359543
2020 PHB interacts with AKT in the mitochondrial sheath of sperm, forming a complex with phospho-PHB (pT258) and phospho-AKT. Blocking PI3K/AKT activity with wortmannin decreases PHB phosphorylation at T258 and reduces sperm motility. Infertile asthenospermic men show reduced phospho-PI3K, phospho-AKT, and phospho-PHB (pT258) levels, suggesting AKT-mediated PHB phosphorylation at T258 regulates sperm motility. Co-localization microscopy, co-immunoprecipitation, pharmacological inhibition (wortmannin), phosphospecific antibodies, human sperm analysis Asian journal of andrology Medium 32859869
2020 HDAC6 downregulates PHB1 expression and function, impairing PHB1-mediated mitochondrial respiratory chain function, increasing oxidant production and oxidative stress, thereby promoting sepsis development. Inhibition of HDAC6 attenuates CLP-induced sepsis through restoration of mitochondrial function. CLP rat sepsis model, HDAC6 inhibition, RT-PCR, Western blot, mitochondrial respiratory control rate measurement Aging Low 32221047
2022 Arctigenin elevates PHB1 protein levels by blocking TRIM21-mediated ubiquitination of PHB1 via estrogen receptor β (ERβ)-dependent competitive interaction: ERβ activation allows it to competitively bind PHB1 and disrupt the TRIM21-PHB1 interaction, preventing PHB1 ubiquitination-mediated degradation and inhibiting mitochondrial apoptosis in goblet cells. In vitro and in vivo colitis models, ERβ knockdown in mice, co-immunoprecipitation (ERβ-PHB1, TRIM21-PHB1), ubiquitination assays, Western blot Phytotherapy research : PTR Medium 35599350
2023 PHB1 (prohibitin) binds to β-catenin and stabilizes it by inhibiting ubiquitin-mediated degradation, thereby increasing Wnt/β-catenin signaling activity and promoting bladder cancer cell EMT, migration, invasion, and metastasis. β-catenin knockdown reduces cancer cell migration and invasion in PHB1-overexpressing cells. Co-immunoprecipitation, immunofluorescence, gene knockdown/overexpression, transwell/wound-healing assays, in vivo lung metastasis nude mouse model, Western blot Pathology, research and practice Medium 37235908
2024 PHB1 interacts with Sam50 (a mitochondrial outer membrane protein) and together they stabilize mtDNA. Lycopene protects against atrazine-induced kidney damage by interacting with Sam50/PHB1 to prevent mtDNA instability, cytoplasmic mtDNA release, and downstream cGAS-STING pathway activation leading to PANoptosis. In vivo mouse model (atrazine + lycopene), Sam50/PHB1 interaction assays, mtDNA stability measurement, mPTP and BAX pore assays, cGAS-STING pathway analysis Journal of agricultural and food chemistry Low 38820047
2024 In Saccharomyces cerevisiae, both Phb1 and Phb2 (orthologs of human PHB1/PHB2) function as Atg8 receptors to support mitophagy via conserved AIM/LIR-like motifs. Phb1 and Phb2 interact with and co-localize with Atg8 at mitochondria. The prohibitin complex also negatively regulates Atg32 processing: in the absence of prohibitins, Atg32 C-terminal processing is enhanced in a manner dependent on the rhomboid protease Pcp1 (and the i-AAA protease Yme1). Yeast Phb1/Phb2 deletion mutants, mitophagy assays, co-immunoprecipitation (Phb1/Phb2 with Atg8), AIM/LIR motif mutagenesis, Atg32 processing assays, genetic epistasis (Yme1, Pcp1 deletion) Autophagy High 38964378
2023 PHBs (PHB1 and PHB2) are directly associated with the eukaryotic initiation factor 4F (eIF4F) translation complex in CLL cells, as shown by multiomics and knockdown experiments. PHB knockdown mimics treatment with the PHB-binding drug FL3, inhibiting MYC oncogene translation and reducing translation of cell cycle and metabolic proteins. The RAS-RAF-(PHB)-MAPK pathway is not implicated in translation regulation in CLL (negative finding in this context). Multiomics (proteomics/transcriptomics), PHB knockdown, FL3 pharmacological inhibition, ribosome profiling/translation assays, in vivo CLL mouse model Blood Medium 37084385

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1993 Tissue response and in vivo degradation of selected polyhydroxyacids: polylactides (PLA), poly(3-hydroxybutyrate) (PHB), and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB/VA). Journal of biomedical materials research 344 8126012
1988 Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli. Journal of bacteriology 295 2848014
2002 The mitochondrial PHB complex: roles in mitochondrial respiratory complex assembly, ageing and degenerative disease. Cellular and molecular life sciences : CMLS 252 11852914
2005 Flotillins and the PHB domain protein family: rafts, worms and anaesthetics. Traffic (Copenhagen, Denmark) 206 16101677
2020 Rational flux-tuning of Halomonas bluephagenesis for co-production of bioplastic PHB and ectoine. Nature communications 102 32620759
2007 The PHB1/2 phosphocomplex is required for mitochondrial homeostasis and survival of human T cells. The Journal of biological chemistry 88 18086671
2005 Novel and economical purification of recombinant proteins: intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association. Protein science : a publication of the Protein Society 88 15883185
2021 Enhanced polyhydroxybutyrate (PHB) production by newly isolated rare actinomycetes Rhodococcus sp. strain BSRT1-1 using response surface methodology. Scientific reports 82 33479335
2011 Identification of a multifunctional protein, PhaM, that determines number, surface to volume ratio, subcellular localization and distribution to daughter cells of poly(3-hydroxybutyrate), PHB, granules in Ralstonia eutropha H16. Molecular microbiology 75 22023320
2017 Controlling cell volume for efficient PHB production by Halomonas. Metabolic engineering 74 28918285
2012 Proliferation and skeletal myotube formation capability of C2C12 and H9c2 cells on isotropic and anisotropic electrospun nanofibrous PHB scaffolds. Biomedical materials (Bristol, England) 71 22477772
2017 Overcoming STC2 mediated drug resistance through drug and gene co-delivery by PHB-PDMAEMA cationic polyester in liver cancer cells. Materials science & engineering. C, Materials for biological applications 69 29208281
2021 The protective role of PHB and its degradation products against stress situations in bacteria. FEMS microbiology reviews 68 33118006
2012 PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha. BMC microbiology 66 23157596
2007 Studies on the influence of phasins on accumulation and degradation of PHB and nanostructure of PHB granules in ralstonia eutropha H16. Biomacromolecules 66 17291089
1993 The human prohibitin (PHB) gene family and its somatic mutations in human tumors. Genomics 66 8244394
2018 Mitochondrial Quality Control Mechanisms and the PHB (Prohibitin) Complex. Cells 64 30501123
2009 PHB/PHBHHx scaffolds and human adipose-derived stem cells for cartilage tissue engineering. Biomaterials 64 19481254
2004 Unraveling the function of the Rhodospirillum rubrum activator of polyhydroxybutyrate (PHB) degradation: the activator is a PHB-granule-bound protein (phasin). Journal of bacteriology 61 15060050
2005 Composite PHB-GGF conduit for long nerve gap repair: a long-term evaluation. Scandinavian journal of plastic and reconstructive surgery and hand surgery 60 16019744
2022 A Review on Enhancing Cupriavidus necator Fermentation for Poly(3-hydroxybutyrate) (PHB) Production From Low-Cost Carbon Sources. Frontiers in bioengineering and biotechnology 59 35928944
2020 Metabolic engineering of Cupriavidus necator H16 for improved chemoautotrophic growth and PHB production under oxygen-limiting conditions. Metabolic engineering 58 32348842
2017 Japanese encephalitis virus induces human neural stem/progenitor cell death by elevating GRP78, PHB and hnRNPC through ER stress. Cell death & disease 56 28102850
1996 Increased PHB productivity by high-cell-density fed-batch culture of Alcaligenes latus, a growth-associated PHB producer. Biotechnology and bioengineering 54 18626935
2017 Deficiency of PHB complex impairs respiratory supercomplex formation and activates mitochondrial flashes. Journal of cell science 53 28630166
2019 LPLUNC1 stabilises PHB1 by counteracting TRIM21-mediated ubiquitination to inhibit NF-κB activity in nasopharyngeal carcinoma. Oncogene 48 30886235
2007 The impact of PHB accumulation on L-glutamate production by recombinant Corynebacterium glutamicum. Journal of biotechnology 48 17555841
2017 Engineering cell wall synthesis mechanism for enhanced PHB accumulation in E. coli. Metabolic engineering 45 29174524
2016 Engineering the growth pattern and cell morphology for enhanced PHB production by Escherichia coli. Applied microbiology and biotechnology 45 27401924
2013 Prohibitin (PHB) inhibits apoptosis in rat granulosa cells (GCs) through the extracellular signal-regulated kinase 1/2 (ERK1/2) and the Bcl family of proteins. Apoptosis : an international journal on programmed cell death 44 24096434
2020 On the Role and Production of Polyhydroxybutyrate (PHB) in the Cyanobacterium Synechocystis sp. PCC 6803. Life (Basel, Switzerland) 40 32331427
2019 FoxM1 Induced Paclitaxel Resistance via Activation of the FoxM1/PHB1/RAF-MEK-ERK Pathway and Enhancement of the ABCA2 Transporter. Molecular therapy oncolytics 40 31334335
2017 Codelivery for Paclitaxel and Bcl-2 Conversion Gene by PHB-PDMAEMA Amphiphilic Cationic Copolymer for Effective Drug Resistant Cancer Therapy. Macromolecular bioscience 40 28714224
2014 Comparative proteome analysis reveals four novel polyhydroxybutyrate (PHB) granule-associated proteins in Ralstonia eutropha H16. Applied and environmental microbiology 39 25548058
2003 Poly(3-hydroxybutyrate) (PHB) synthesis by recombinant Escherichia coli harbouring Streptomyces aureofaciens PHB biosynthesis genes: effect of various carbon and nitrogen sources. Microbiological research 39 12608576
2016 PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs. Cell stem cell 38 27939217
1997 Megaplasmid and chromosomal loci for the PHB degradation pathway in Rhizobium (Sinorhizobium) meliloti. Genetics 38 9258668
2018 Polyhydroxybutyrate (PHB) biodegradation using bacterial strains with demonstrated and predicted PHB depolymerase activity. Applied microbiology and biotechnology 37 29951858
2017 Controlling microbial PHB synthesis via CRISPRi. Applied microbiology and biotechnology 36 28620688
2009 Statistical media optimization for growth and PHB production from methanol by a methylotrophic bacterium. Bioresource technology 36 19121581
2014 Histological and molecular-biological analyses of poly(3-hydroxybutyrate) (PHB) patches for enhancement of bone regeneration. Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 33 24862689
2013 Development and characterization of a PHB-HV-based 3D scaffold for a tissue engineering and cell-therapy combinatorial approach for spinal cord injury regeneration. Macromolecular bioscience 33 24038969
2009 Purification, immobilization, and characterization of nattokinase on PHB nanoparticles. Bioresource technology 33 19608412
2009 Production and characterization of PHB from two novel strains of Bacillus spp. isolated from soil and activated sludge. Journal of industrial microbiology & biotechnology 32 19953293
2023 PHB production from food waste hydrolysates by Halomonas bluephagenesis Harboring PHB operon linked with an essential gene. Metabolic engineering 30 36889504
2007 Influence of the poly-3-hydroxybutyrate (PHB) granule-associated proteins (PhaP1 and PhaP2) on PHB accumulation and symbiotic nitrogen fixation in Sinorhizobium meliloti Rm1021. Journal of bacteriology 30 17921298
2021 Improvement of polyhydroxybutyrate (PHB) plate-based screening method for PHB degrading bacteria using cell-grown amorphous PHB and recovered by sodium dodecyl sulfate (SDS). International journal of biological macromolecules 29 33607129
2017 Effects of dietary poly-β-hydroxybutyrate (PHB) on microbiota composition and the mTOR signaling pathway in the intestines of litopenaeus vannamei. Journal of microbiology (Seoul, Korea) 29 29214487
2011 Prohibitin (PHB) acts as a potent survival factor against ceramide induced apoptosis in rat granulosa cells. Life sciences 29 21763324
2007 Engineering of PHB synthesis causes improved elastic properties of flax fibers. Biotechnology progress 28 17269698
2019 Enhanced production of poly‑3‑hydroxybutyrate (PHB) by expression of response regulator DR1558 in recombinant Escherichia coli. International journal of biological macromolecules 27 30851327
2020 The Growth of 3T3 Fibroblasts on PHB, PLA and PHB/PLA Blend Films at Different Stages of Their Biodegradation In Vitro. Polymers 26 33383857
2022 CHCHD10 and SLP2 control the stability of the PHB complex: a key factor for motor neuron viability. Brain : a journal of neurology 25 35656794
2013 Novel extracellular PHB depolymerase from Streptomyces ascomycinicus: PHB copolymers degradation in acidic conditions. PloS one 24 23951224
2012 Association of PHB 1630 C>T and MTHFR 677 C>T polymorphisms with breast and ovarian cancer risk in BRCA1/2 mutation carriers: results from a multicenter study. British journal of cancer 24 22669161
2004 The activator of the Rhodospirillum rubrum PHB depolymerase is a polypeptide that is extremely resistant to high temperature (121 degrees C) and other physical or chemical stresses. FEMS microbiology letters 24 14757249
2024 Lycopene Protects against Atrazine-Induced Kidney STING-Dependent PANoptosis through Stabilizing mtDNA via Interaction with Sam50/PHB1. Journal of agricultural and food chemistry 23 38820047
2023 Plasma surface modification of electrospun polyhydroxybutyrate (PHB) nanofibers to investigate their performance in bone tissue engineering. International journal of biological macromolecules 23 36621738
2023 PHB promotes bladder cancer cell epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway. Pathology, research and practice 23 37235908
2020 PHB regulates meiotic recombination via JAK2-mediated histone modifications in spermatogenesis. Nucleic acids research 23 32232334
2018 Halomonas smyrnensis as a cell factory for co-production of PHB and levan. International journal of biological macromolecules 23 30001608
2023 Polyhydroxybutyrate (PHB) production from sugar cane molasses and tap water without sterilization using novel strain, Priestia sp. YH4. International journal of biological macromolecules 22 37558031
2016 Effect of Polyhydroxybutyrate (PHB) storage on L-arginine production in recombinant Corynebacterium crenatum using coenzyme regulation. Microbial cell factories 22 26785743
2014 To be or not to be a poly(3-hydroxybutyrate) (PHB) depolymerase: PhaZd1 (PhaZ6) and PhaZd2 (PhaZ7) of Ralstonia eutropha, highly active PHB depolymerases with no detectable role in mobilization of accumulated PHB. Applied and environmental microbiology 22 24907326
2006 Isolation of a Gram-positive poly(3-hydroxybutyrate) (PHB)-degrading bacterium from compost, and cloning and characterization of a gene encoding PHB depolymerase of Bacillus megaterium N-18-25-9. FEMS microbiology letters 22 17064368
2023 Poly(3-hydroxybutyrate) (PHB) and Polycaprolactone (PCL) Based Blends for Tissue Engineering and Bone Medical Applications Processed by FDM 3D Printing. Polymers 21 37242979
2023 The landscape of mitophagy in sepsis reveals PHB1 as an NLRP3 inflammasome inhibitor. Frontiers in immunology 21 37359543
2022 Effect of recombinant BMP-2 and erythropoietin on osteogenic properties of biomimetic PLA/PCL/HA and PHB/HA scaffolds in critical-size cranial defects model. Materials science & engineering. C, Materials for biological applications 21 35581092
2018 Bradyrhizobium diazoefficiens USDA110 PhaR functions for pleiotropic regulation of cellular processes besides PHB accumulation. BMC microbiology 21 30355296
2023 Inhibition of MYC translation through targeting of the newly identified PHB-eIF4F complex as a therapeutic strategy in CLL. Blood 20 37084385
2019 Purification and kinetics of the PHB depolymerase of Microbacterium paraoxydans RZS6 isolated from a dumping yard. PloS one 20 31211775
2019 Prohibitin (PHB) expression is associated with aggressiveness in DLBCL and flavagline-mediated inhibition of cytoplasmic PHB functions induces anti-tumor effects. Journal of experimental & clinical cancer research : CR 20 31684984
2017 Pseudogene PHBP1 promotes esophageal squamous cell carcinoma proliferation by increasing its cognate gene PHB expression. Oncotarget 20 28404970
2022 Phytoestrogen arctigenin preserves the mucus barrier in inflammatory bowel diseases by inhibiting goblet cell apoptosis via the ERβ/TRIM21/PHB1 pathway. Phytotherapy research : PTR 18 35599350
2021 STOML2 interacts with PHB through activating MAPK signaling pathway to promote colorectal Cancer proliferation. Journal of experimental & clinical cancer research : CR 18 34781982
2018 Flavagline analog FL3 induces cell cycle arrest in urothelial carcinoma cell of the bladder by inhibiting the Akt/PHB interaction to activate the GADD45α pathway. Journal of experimental & clinical cancer research : CR 18 29415747
2004 Roles of poly(3-hydroxybutyrate) depolymerase and 3HB-oligomer hydrolase in bacterial PHB metabolism. Current microbiology 18 15170237
2023 Polyhydroxybutyrate (PHB) in nanoparticulate form improves physical and biological performance of scaffolds. International journal of biological macromolecules 17 36870657
2022 Isolation and optimization of extracellular PHB depolymerase producer Aeromonas caviae Kuk1-(34) for sustainable solid waste management of biodegradable polymers. PloS one 17 35421107
2020 HDAC6 promotes sepsis development by impairing PHB1-mediated mitochondrial respiratory chain function. Aging 17 32221047
2014 A disease-causing mutation illuminates the protein membrane topology of the kidney-expressed prohibitin homology (PHB) domain protein podocin. The Journal of biological chemistry 17 24596097
2010 Comparative genome analysis of PHB gene family reveals deep evolutionary origins and diverse gene function. BMC bioinformatics 17 20946606
2022 Novel approach based on GQD-PHB as anchoring platform for the development of SARS-CoV-2 electrochemical immunosensor. Analytica chimica acta 16 36257733
2020 Biocompatible PHB Production from Bacillus Species Under Submerged and Solid-State Fermentation and Extraction Through Different Downstream Processing. Current microbiology 16 32095890
2020 Preparation and characterisation of organic UV filters based on combined PHB/liposomes with natural phenolic compounds. Journal of biotechnology 16 34154737
2014 Impact of Ralstonia eutropha's poly(3-Hydroxybutyrate) (PHB) Depolymerases and Phasins on PHB storage in recombinant Escherichia coli. Applied and environmental microbiology 16 25281380
2022 Polyhydroxybutyrate (PHB)-Based Biodegradable Polymer from Agromyces indicus: Enhanced Production, Characterization, and Optimization. Polymers 15 36235929
2016 Prohibitin( PHB) roles in granulosa cell physiology. Cell and tissue research 15 26496733
2023 Osteogenic potential of PHB-lignin/cellulose nanofiber electrospun scaffold as a novel bone regeneration construct. International journal of biological macromolecules 14 37532195
2022 PHB production from cellobiose with Saccharomyces cerevisiae. Microbial cell factories 14 35729556
2024 Prohibitins, Phb1 and Phb2, function as Atg8 receptors to support yeast mitophagy and also play a negative regulatory role in Atg32 processing. Autophagy 13 38964378
2023 Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds with Different Additives of ATBC and OLA Plasticizers. Journal of functional biomaterials 13 37623657
2021 Polyhydroxybutyrate (PHB) Production Using an Arabinose-Inducible Expression System in Comparison With Cold Shock Inducible Expression System in Escherichia coli. Frontiers in bioengineering and biotechnology 13 34012957
2016 Backup Expression of the PhaP2 Phasin Compensates for phaP1 Deletion in Herbaspirillum seropedicae, Maintaining Fitness and PHB Accumulation. Frontiers in microbiology 13 27242754
2011 Identification and characterization of PhbF: a DNA binding protein with regulatory role in the PHB metabolism of Herbaspirillum seropedicae SmR1. BMC microbiology 13 21999748
2021 Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources. Polymers 12 34771358
2020 Prohibitin (PHB) interacts with AKT in mitochondria to coordinately modulate sperm motility. Asian journal of andrology 12 32859869
2017 PHB in Cardiovascular and Other Diseases: Present Knowledge and Implications. Current drug targets 12 27557820
2022 Characterization of Polyhydroxybutyrate, PHB, Synthesized by Newly Isolated Haloarchaea Halolamina spp. Molecules (Basel, Switzerland) 11 36364191
2009 Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter. Plasmid 11 19699761

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