Affinage

Showing PHF12 — PF1 is a alias.

PHF12

PHD finger protein 12 · UniProt Q96QT6

Length
1004 aa
Mass
109.7 kDa
Annotated
2026-06-10
82 papers in source corpus 9 papers cited in narrative 10 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PHF12 (Pf1) is a dual-PHD zinc finger adaptor protein that scaffolds the Rpd3S/Sin3S histone deacetylase corepressor complex and directs it to chromatin to repress transcription (PMID:11390640, PMID:12391155). It engages mSin3A through two independent Sin3-interaction domains: SID1 binds the PAH2 domain in a Mad/Mxd1-like mode (resolved by NMR), while SID2 binds PAH1 as a distinct novel module (PMID:11390640, PMID:21440557). Within the MORF family, PHF12 selectively binds MRG15 (not MRGX or MORF4), and because PHF12 and MRG15 contact different surfaces of mSin3A it assembles a trimeric MRG15/PHF12/mSin3A complex (PMID:12391155); notably, MRG15 and the Sin3 PAH2 domain compete for an overlapping PHF12 segment, indicating dynamic rather than simultaneous engagement of these subunits (PMID:21440557). Chromatin targeting is achieved bivalently: the PHF12 PHD1 domain binds the unmodified histone H3 N-terminus (H3K4me0) while the MRG15 chromodomain reads a separate H3 mark, each at low affinity, so that co-engagement is required for stable recruitment (PMID:22728643). A polybasic region immediately C-terminal to PHD1 additionally binds phosphoinositides, most strongly PI(3)P (PMID:16893883). Beyond corepression, PHF12 sustains proliferative and invasive programs in cancer: it is required for maintenance of stem-cell and EMT phenotypes in triple-negative breast cancer via the SIN3A-PHF12 interaction and downstream KLF9-mediated control of ITGA6/ITGB1 (PMID:26460951, PMID:34968869), and it transcriptionally upregulates HDAC1 to activate EGFR/AKT signaling in non-small cell lung cancer (PMID:39075515). Genetic inactivation of Phf12 in mice causes mid-to-late gestation lethality, and Phf12-null fibroblasts display impaired proliferation, cellular senescence, elevated DNA-damage marking, disrupted nucleolar morphology, and impaired ribosome biogenesis, with PHF12 complexes enriched for ribosome biogenesis factors (PMID:27956701).

Mechanistic history

Synthesis pass · year-by-year structured walk · 8 steps
  1. 2001 High

    Established PHF12 as a transcriptional corepressor adaptor by showing it physically recruits the mSin3A HDAC complex through two independent Sin3-interaction domains, defining its core repressive function.

    Evidence Co-IP, GST pulldown domain mapping, and Gal4-fusion repression assays in mammalian cells

    PMID:11390640

    Open questions at the time
    • Did not resolve how SID1/SID2 dual binding affects complex stoichiometry
    • No chromatin target genes identified
    • TLE bridging (same paper) rests on Co-IP/reporter assay only
  2. 2002 High

    Defined the selectivity of PHF12 within the MORF family and showed it forms a defined trimeric complex, clarifying which corepressor module it assembles.

    Evidence Reciprocal Co-IP, GST pulldown domain mapping, and Gal4-luciferase repression assays

    PMID:12391155

    Open questions at the time
    • Structural basis of MRG15 selectivity over MRGX/MORF4 not resolved
    • Endogenous complex composition in chromatin context not shown
  3. 2006 High

    Revealed an unexpected lipid-binding activity for PHF12, showing a polybasic region adjacent to PHD1 confers specific PI(3)P binding and linking the protein to phosphoinositide signaling.

    Evidence In vitro lipid-protein binding assays, fusion pulldowns, and domain-swap experiments

    PMID:16893883

    Open questions at the time
    • Cellular function of PI(3)P binding for PHF12 not established
    • Whether lipid and histone binding by PHD1 are mutually exclusive unknown
  4. 2011 High

    Provided atomic-resolution detail of the PHF12 SID1–mSin3A PAH2 interface and uncovered competition between MRG15 and PAH2 for PHF12, reframing how the complex subunits engage the adaptor.

    Evidence NMR solution structure, ITC, and competitive binding assays

    PMID:21440557

    Open questions at the time
    • Functional consequence of MRG15/PAH2 competition in vivo untested
    • Did not address the SID2-PAH1 interaction structurally
  5. 2012 High

    Demonstrated that PHF12 PHD1 and MRG15 chromodomain co-read distinct H3 marks with low individual affinity, establishing bivalent recognition as the mechanism for targeting Rpd3S/Sin3S to chromatin.

    Evidence Fluorescence polarization, ITC, peptide pulldowns, and mutagenesis

    PMID:22728643

    Open questions at the time
    • In vivo genome-wide chromatin occupancy not mapped
    • Link between bivalent reading and suppression of specific transcripts not directly shown
  6. 2017 High

    Defined the organismal and cellular requirement for PHF12, showing it is essential for embryonic viability and for preventing senescence, and linked it unexpectedly to nucleolar integrity and ribosome biogenesis.

    Evidence Knockout mouse, MEF phenotyping (proliferation, SA-β-Gal, γ-H2A.X, nucleolar morphology), proteomics, and transcriptomics

    PMID:27956701

    Open questions at the time
    • Mechanistic link between corepressor scaffolding and ribosome biogenesis not resolved
    • Whether nucleolar phenotype is direct or downstream of senescence unclear
  7. 2021 Medium

    Placed the SIN3A-PHF12 complex in a defined oncogenic circuit, showing it represses KLF9-controlled integrin genes to govern invasion in triple-negative breast cancer.

    Evidence ChIP for SIN3A/KLF9 occupancy, RNA-seq, KLF9 knockdown rescue, and invasion/migration assays

    PMID:34968869

    Open questions at the time
    • Direct PHF12 promoter binding at ITGA6/ITGB1 not shown
    • Generality beyond TNBC untested
  8. 2024 Medium

    Identified a transcriptional output of PHF12 in lung cancer, showing it binds the HDAC1 promoter to upregulate HDAC1 and activate EGFR/AKT signaling, distinct from its classical corepressor role.

    Evidence ChIP at HDAC1 promoter, knockdown/overexpression rescue, RNA-seq/GSEA, and xenograft model

    PMID:39075515

    Open questions at the time
    • Single lab, single paper without reciprocal validation
    • How a corepressor adaptor activates HDAC1 transcription mechanistically unresolved
    • Direct versus indirect effect on EGFR/AKT not separated

Open questions

Synthesis pass · forward-looking unresolved questions
  • How PHF12's chromatin-corepressor scaffolding function mechanistically connects to ribosome biogenesis, embryonic viability, and its context-dependent oncogenic roles remains unresolved.
  • No unified model linking corepression to nucleolar/ribosome phenotypes
  • Physiological role of PI(3)P binding undefined
  • Genome-wide direct target catalogue lacking

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 3 GO:0140110 transcription regulator activity 2 GO:0008289 lipid binding 1 GO:0042393 histone binding 1
Localization
GO:0005634 nucleus 2 GO:0005730 nucleolus 1
Pathway
R-HSA-74160 Gene expression (Transcription) 3 R-HSA-4839726 Chromatin organization 2 R-HSA-1852241 Organelle biogenesis and maintenance 1
Partners
HDAC1MRG15SIN3ATLE
Complex memberships
MRG15/PHF12/mSin3A trimeric complexRpd3S/Sin3S HDAC corepressor complex

Evidence

Reading pass · 10 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 PHF12 (Pf1) was identified as a novel PHD zinc finger protein that physically associates with the mSin3A histone deacetylase corepressor complex in vivo and recruits it to repress transcription. PHF12 contains two independent Sin3 interaction domains (SID1 and SID2): SID1 binds the PAH2 domain of mSin3A (with sequence similarity to the Mad SID), while SID2 binds the PAH1 domain of mSin3A and represents a novel Sin3-binding module. Co-immunoprecipitation, GST pulldown/mapping experiments, Gal4-fusion transcriptional repression assays in mammalian cells Molecular and cellular biology High 11390640
2001 PHF12 (Pf1) also interacts with the mammalian Groucho homolog TLE (transducin-like enhancer of split) corepressor in an mSin3A-independent manner and recruits functional TLE complexes to repress transcription, suggesting PHF12 bridges two global corepressor networks. Co-immunoprecipitation, Gal4-fusion transcriptional repression assays Molecular and cellular biology Medium 11390640
2002 PHF12 (Pf1) selectively interacts with MRG15 (but not MRGX or MORF4) among the MORF family of transcriptional regulators. PHF12 has independent binding sites for MRG15 and mSin3A, and MRG15 and PHF12 bind different domains on mSin3A, forming a trimeric MRG15/Pf1/mSin3A complex. PHF12 reduces transcriptional repression by Gal4-MRG15 but has no effect on MRGX or MORF4-mediated repression. Co-immunoprecipitation, GST pulldown domain mapping, Gal4-fusion luciferase reporter repression assays, dominant-negative TLE experiments Molecular and cellular biology High 12391155
2006 The PHD1 domain of PHF12 (Pf1), together with a polybasic region immediately C-terminal to PHD1, binds phosphoinositides, most strongly PI(3)P. The polybasic region alone is necessary and sufficient for specific PI(3)P binding when fused to heterologous proteins or as an isolated peptide; swapping polybasic regions between PHD fingers transfers PI-binding specificity. Lipid-protein binding assays (PI strip/dot blot), GST/MBP fusion pulldowns with phosphoinositides, peptide binding experiments, domain-swap experiments The Journal of biological chemistry High 16893883
2011 The NMR solution structure of the mSin3A PAH2 domain bound to the PHF12 SID1 motif was determined, revealing a Mad1/Mxd1-like interaction mode. Unexpectedly, MRG15 competes with Sin3 PAH2 for the same PHF12 segment encompassing SID1 and a conserved adjacent motif, implying competitive rather than cooperative binding between two subunits of the same Rpd3S/Sin3S complex for PHF12. NMR structure determination, isothermal titration calorimetry (ITC), competitive binding assays Journal of molecular biology High 21440557
2012 The PHD1 domain of PHF12 binds preferentially to the unmodified extreme N-terminus of histone H3 (H3K4me0) but not to H3K4me2/3. PHF12 PHD1 and MRG15 chromodomain each bind their respective histone H3 targets with >100 µM affinity, requiring bivalent (not cooperative) co-engagement to achieve biologically significant chromatin targeting of the Rpd3S/Sin3S complex. PHF12 PHD1 also engages the MRG15 MRG domain in a PHF12 MRG-binding-domain-dependent manner. Fluorescence polarization binding assays, isothermal titration calorimetry, peptide pulldowns, mutagenesis Journal of molecular biology High 22728643
2015 Disruption of the SIN3A–PHF12 interaction (via a competitive Tat-SID peptide mimicking the SIN3 interaction domain of MAD) blocked PHF12 binding to SIN3A PAH2, leading to epigenetic modulation and transcriptional downregulation of TNBC stem cell and EMT markers. PHF12 knockdown phenocopied Tat-SID treatment, reducing primary tumor growth and metastasis in vivo, establishing PHF12 as required for maintenance of the stem cell phenotype and EMT in triple-negative breast cancer. Competitive peptide displacement of SIN3A-PHF12 interaction, PHF12 shRNA knockdown, in vitro functional assays (proliferation, sphere formation), in vivo xenograft tumor model Oncotarget Medium 26460951
2017 Genetic inactivation of Phf12 (Pf1) in mice causes mid-to-late gestation lethality. In mouse embryonic fibroblasts, Phf12 loss impairs proliferation, increases senescence-associated β-galactosidase activity (cellular senescence), elevates γ-H2A.X (DNA double-strand break marker), and disrupts nucleolar morphology. Proteomic analysis of PHF12-interacting complexes revealed enrichment of ribosome biogenesis factors, and transcript profiling showed PHF12 impacts multiple ribosome biogenesis regulatory pathways. Conditional/constitutive knockout mouse generation, MEF proliferation assays (BrdU incorporation), SA-β-Gal senescence assay, γ-H2A.X immunostaining, nucleolar morphology analysis, mass spectrometry-based proteomics of PHF12 complexes, transcriptomic profiling Molecular and cellular biology High 27956701
2021 Disruption of the PF1/SIN3A interaction (via PF1-SID expression) leads to increased occupancy of SIN3A and the transcriptional repressor KLF9 on the promoters of ITGA6 and ITGB1, reducing their expression. KLF9 knockdown restores ITGA6/ITGB1 expression and the invasive phenotype, placing KLF9 downstream of the SIN3A-PHF12 complex in suppression of invasion and migration in TNBC. ChIP assay (SIN3A and KLF9 promoter occupancy), RNA-seq transcriptomics, KLF9 knockdown rescue experiments, invasion/migration assays Translational oncology Medium 34968869
2024 PHF12 transcriptionally regulates HDAC1 expression: ChIP assay showed PHF12 binds the HDAC1 promoter region. PHF12 knockdown reduces HDAC1 mRNA and protein levels, and HDAC1 overexpression rescues proliferation and migration in PHF12-knockdown NSCLC cells. The PHF12-HDAC1 axis activates the EGFR/AKT signaling pathway in non-small cell lung cancer. ChIP assay (PHF12 binding at HDAC1 promoter), qRT-PCR/Western blot (HDAC1 mRNA and protein), siRNA knockdown, HDAC1 overexpression rescue, RNA-seq + GSEA pathway analysis, in vivo xenograft model Journal of translational medicine Medium 39075515

Source papers

Stage 0 corpus · 82 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1983 Structure similarity, difference and variability in the filamentous viruses fd, If1, IKe, Pf1 and Xf. Investigation by laser Raman spectroscopy. Journal of molecular biology 135 6405045
1991 NMR studies of the structure and dynamics of membrane-bound bacteriophage Pf1 coat protein. Science (New York, N.Y.) 121 1925542
2011 Chemical shifts for the unusual DNA structure in Pf1 bacteriophage from dynamic-nuclear-polarization-enhanced solid-state NMR spectroscopy. Journal of the American Chemical Society 84 21854063
2002 Role for the mortality factors MORF4, MRGX, and MRG15 in transcriptional repression via associations with Pf1, mSin3A, and Transducin-Like Enhancer of Split. Molecular and cellular biology 84 12391155
2007 Filamentous phage studied by magic-angle spinning NMR: resonance assignment and secondary structure of the coat protein in Pf1. Journal of the American Chemical Society 83 17279748
1983 Comparison of protein and deoxyribonucleic acid backbone structures in fd and Pf1 bacteriophages. Biochemistry 82 6340725
2004 Structure of the coat protein in Pf1 bacteriophage determined by solid-state NMR spectroscopy. Journal of molecular biology 81 15288792
2008 Conformational dynamics of an intact virus: order parameters for the coat protein of Pf1 bacteriophage. Proceedings of the National Academy of Sciences of the United States of America 79 18653759
1991 DNA sequence of the filamentous bacteriophage Pf1. Journal of molecular biology 79 2010913
1986 The ionic properties of the filamentous bacteriophages Pf1 and fd. The Journal of biological chemistry 77 3944103
1994 Pf1 virus structure: helical coat protein and DNA with paraxial phosphates. Science (New York, N.Y.) 75 8036516
2001 Pf1, a novel PHD zinc finger protein that links the TLE corepressor to the mSin3A-histone deacetylase complex. Molecular and cellular biology 71 11390640
2006 The polybasic region that follows the plant homeodomain zinc finger 1 of Pf1 is necessary and sufficient for specific phosphoinositide binding. The Journal of biological chemistry 67 16893883
2010 Structure and dynamics of the membrane-bound form of Pf1 coat protein: implications of structural rearrangement for virus assembly. Biophysical journal 58 20816058
1991 Membrane-mediated assembly of filamentous bacteriophage Pf1 coat protein. Science (New York, N.Y.) 52 1925543
1975 Structure of coat proteins in Pf1 and fd virions by laser raman spectroscopy. Science (New York, N.Y.) 52 1170637
1994 PF1: an A-T hook-containing DNA binding protein from rice that interacts with a functionally defined d(AT)-rich element in the oat phytochrome A3 gene promoter. The Plant cell 51 8148649
2000 Intensity of the polarized Raman band at 1340-1345 cm-1 as an indicator of protein alpha-helix orientation: application to Pf1 filamentous virus. Biochemistry 44 10704218
1979 Macromolecular structural transitions in Pf1 filamentous bacterial virus. Nature 41 481592
1987 Structure and dynamics of the Pf1 filamentous bacteriophage coat protein in micelles. Biochemistry 40 3567175
1999 Demonstration by ultraviolet resonance Raman spectroscopy of differences in DNA organization and interactions in filamentous viruses Pf1 and fd. Biochemistry 39 10074370
2000 The molecular structure and structural transition of the alpha-helical capsid in filamentous bacteriophage Pf1. Acta crystallographica. Section D, Biological crystallography 36 10666593
1995 Pf1 filamentous bacteriophage: refinement of a molecular model by simulated annealing using 3.3 A resolution X-ray fibre diffraction data. Acta crystallographica. Section D, Biological crystallography 35 15299811
1989 A CD determination of the alpha-helix contents of the coat proteins of four filamentous bacteriophages: fd, IKe, Pf1, and Pf3. Biopolymers 35 2597737
2005 Structural basis of the temperature transition of Pf1 bacteriophage. Protein science : a publication of the Protein Society 34 15741342
1994 Ultraviolet absorbance and circular dichroism of Pf1 virus: nucleotide/subunit ratio of unity, hyperchromic tyrosines and DNA bases, and high helicity in the subunits. Biochemistry 33 8110771
1987 Pf1 Inovirus. Electron density distribution calculated by a maximum entropy algorithm from native fibre diffraction data to 3 A resolution and single isomorphous replacement data to 5 A resolution. Journal of molecular biology 33 3599076
1983 Silver and mercury probing of deoxyribonucleic acid structures in the filamentous viruses fd, If1, IKe, Xf, Pf1, and Pf3. Biochemistry 33 6354268
1983 Maximum-entropy calculation of the electron density at 4 A resolution of Pf1 filamentous bacteriophage. Proceedings of the National Academy of Sciences of the United States of America 32 6410396
1982 The DNA-binding protein of Pf1 filamentous bacteriophage: amino-acid sequence and structure of the gene. The EMBO journal 32 16453414
1982 DNA packing in the filamentous viruses fd, Xf, Pf1 and Pf3. Nucleic acids research 31 6806774
1991 Neutron diffraction studies of the structure of filamentous bacteriophage Pf1. Demonstration that the coat protein consists of a pair of alpha-helices with an intervening, non-helical surface loop. Journal of molecular biology 30 1906942
1988 Sugar pucker and phosphodiester conformations in viral genomes of filamentous bacteriophages: fd, If1, IKe, Pf1, Xf, and Pf3. Biochemistry 29 3166982
2011 Solution structure of the mSin3A PAH2-Pf1 SID1 complex: a Mad1/Mxd1-like interaction disrupted by MRG15 in the Rpd3S/Sin3S complex. Journal of molecular biology 28 21440557
2015 Targeting the SIN3A-PF1 interaction inhibits epithelial to mesenchymal transition and maintenance of a stem cell phenotype in triple negative breast cancer. Oncotarget 26 26460951
2003 Protein and DNA residue orientations in the filamentous virus Pf1 determined by polarized Raman and polarized FTIR spectroscopy. Biochemistry 26 12549913
1999 The HMG-I/Y protein PF1 stimulates binding of the transcriptional activator GT-2 to the PHYA gene promoter. The Plant journal : for cell and molecular biology 26 10363369
1995 A Monte Carlo model of fd and Pf1 coat proteins in lipid membranes. Biophysical journal 26 8534808
1995 The effect of the nematode peptides SDPNFLRFamide (PF1) and SADPNFLRFamide (PF2) on synaptic transmission in the parasitic nematode Ascaris suum. Parasitology 25 7753584
1993 Evaluation of transmembrane helix prediction methods using the recently defined NMR structures of the coat proteins from bacteriophages M13 and Pf1. Biochimica et biophysica acta 24 8373820
2012 Sequence requirements for combinatorial recognition of histone H3 by the MRG15 and Pf1 subunits of the Rpd3S/Sin3S corepressor complex. Journal of molecular biology 23 22728643
2014 Pf1 bacteriophage hydration by magic angle spinning solid-state NMR. The Journal of chemical physics 21 25494804
2002 Structure-activity relationships of an inhibitory nematode FMRFamide-related peptide, SDPNFLRFamide (PF1), on Ascaris suum muscle. International journal for parasitology 21 12464423
1988 Conformation of the coat protein of filamentous bacteriophage Pf1 determined by neutron diffraction from magnetically oriented gels of specifically deuterated virions. Journal of molecular biology 21 3127590
2015 Purification and Characterization of a Fucoidanase (FNase S) from a Marine Bacterium Sphingomonas paucimobilis PF-1. Marine drugs 19 26193285
1991 Circular dichroism and fluorescence analysis of the interaction of Pf1 gene 5 protein with poly(dT). Journal of molecular biology 19 2005618
2010 A structural model for the single-stranded DNA genome of filamentous bacteriophage Pf1. Biochemistry 18 20078135
1985 Time-resolved fluorescence of bacteriophage Pf1 DNA-binding protein. Determination of oligonucleotide and polynucleotide binding parameters. European journal of biochemistry 18 4039662
2013 NMR-based simulation studies of Pf1 coat protein in explicit membranes. Biophysical journal 17 23931317
2004 Effects of virion and salt concentrations on the Raman signatures of filamentous phages fd, Pf1, Pf3, and PH75. Biochemistry 17 15476406
1998 Analysis of X-ray diffraction from fibres of Pf1 Inovirus (filamentous bacteriophage) shows that the DNA in the virion is not highly ordered. Journal of molecular biology 17 9878347
2021 Biodegradation of organophosphorus pesticide profenofos by the bacterium Bacillus sp. PF1 and elucidation of initial degradation pathway. Environmental technology 16 34469281
2017 The Chromatin-Associated Phf12 Protein Maintains Nucleolar Integrity and Prevents Premature Cellular Senescence. Molecular and cellular biology 16 27956701
2021 Invasive phenotype in triple negative breast cancer is inhibited by blocking SIN3A-PF1 interaction through KLF9 mediated repression of ITGA6 and ITGB1. Translational oncology 15 34968869
1995 FT-IR spectroscopy of the major coat protein of M13 and Pf1 in the phage and reconstituted into phospholipid systems. Biochemistry 15 7794893
1988 Raman spectroscopy of mercury (II) binding to two filamentous viruses: Ff (fd, M13, f1) and Pf1. Biochemistry 15 3280025
2016 Small Colony Variants and Single Nucleotide Variations in Pf1 Region of PB1 Phage-Resistant Pseudomonas aeruginosa. Frontiers in microbiology 14 27014207
2010 Simultaneous structure and dynamics of a membrane protein using REDCRAFT: membrane-bound form of Pf1 coat protein. Journal of magnetic resonance (San Diego, Calif. : 1997) 14 20829084
1993 Biophysical studies of the Pf1 coat protein in the filamentous phage, in detergent micelles, and in a membrane environment. Biochemistry 14 8399218
1991 The role of tyrosine residues in the DNA-binding site of the Pf1 gene 5 protein. Protein engineering 14 1891463
1987 Structural responsiveness of filamentous bacteriophage Pf1: comparison of virion structure in fibers and solution. The effect of temperature and ionic strength. Biophysical journal 14 3663828
1985 Natural killer (NK) cells and graft-versus-host disease (GVHD): no correlation between the NK cell levels and GVHD in the murine P----F1 model. Immunology 14 3972429
2014 Involvement of tumor-associated macrophage activation in vitro during development of a novel mantle cell lymphoma cell line, PF-1, derived from a typical patient with relapsed disease. Leukemia & lymphoma 12 24611650
2000 Pf1 filamentous phage as an alignment tool for generating local and global structural information in nucleic acids. Journal of biomolecular structure & dynamics 12 22607445
1983 Dissociation of the Pf1 nucleoprotein assembly complex and characterisation of the DNA binding protein. Biochimica et biophysica acta 12 6297583
1993 Molecular dynamics simulation of Pf1 coat protein. Biophysical journal 10 8471719
1998 Equilibrium and kinetic binding analysis of the N-terminal domain of the Pf1 gene 5 protein and its interaction with single-stranded DNA. Journal of molecular biology 9 9466922
1985 Identification of lysine residues at the binding site of bacteriophage-Pf1 DNA-binding protein. The Biochemical journal 8 3924033
1999 Conformational studies of the C-terminal domain of bacteriophage Pf1 gene 5 protein. Biochimica et biophysica acta 7 10561545
1995 Comparison of Pf1 and Fd gene 5 proteins and their single-stranded DNA complexes by NMR spectroscopy and differential scanning calorimetry. Biochemistry 7 7819190
1987 Cloning and expression of the filamentous bacteriophage Pf1 major coat protein gene in Escherichia coli. Membrane protein processing and virus assembly. Journal of molecular biology 7 3309343
2021 LncRNA RP11-116G8.5 promotes the progression of lung squamous cell carcinoma through sponging miR-3150b-3p/miR-6870-5p to upregulate PHF12/FOXP4. Pathology, research and practice 6 34500373
1993 Identification of a compact DNA-binding domain in the gene 5 protein of Pf1 bacteriophage. Biochemistry 6 8466905
1988 Thermal difference circular dichroism of Pf1 filamentous virus and effects of mercury(II), silver(I), and copper(II). Biochemistry 6 3136800
1984 Estimation of tyrosine-40-DNA distance in the filamentous phage Pf1 by analysis of its intrinsic fluorescence properties. Biochimica et biophysica acta 5 6477921
1983 Pf1 bacteriophage replication--assembly complex. X-ray fibre diffraction of the high humidity form. Journal of molecular biology 5 6606711
2008 Effects of SDPNFLRF-amide (PF1) on voltage-activated currents in Ascaris suum muscle. International journal for parasitology 4 18760280
1991 Export of infectious particles by Escherichia coli transfected with the RF DNA of Pf1, a virus of Pseudomonas aeruginosa strain K. Molecular microbiology 4 1779755
1994 Cloning and characterization of cDNAs encoding oat PF1: a protein that binds to the PE1 region in the oat phytochrome A3 gene promoter. Biochemical Society symposium 3 7639786
2024 PHF12 regulates HDAC1 to promote tumorigenesis via EGFR/AKT signaling pathway in non-small cell lung cancer. Journal of translational medicine 2 39075515
2004 Line shape analyses for water 17O NMR quintet observed in a bacteriophage Pf1 solution at different temperatures. Biophysical chemistry 2 14967240
1991 Structural parameters of the Pf1 gene 5 protein-DNA complex in solution by neutron scattering. Journal of molecular biology 1 1942028

Missed literature

Know a paper Affinage missed for PHF12? Flag it for the maintainers and the community.

No submissions yet.