Affinage

Showing NSMCE3 — NSE3 is a alias.

NSMCE3

Non-structural maintenance of chromosomes element 3 homolog · UniProt Q96MG7

Length
304 aa
Mass
34.3 kDa
Annotated
2026-06-10
46 papers in source corpus 23 papers cited in narrative 23 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NSMCE3 (MAGEG1/NSE3) is a KITE-family subunit of the SMC5/6 genome-maintenance complex that is essential for chromosome segregation, homologous recombination repair, and resistance to DNA damage and replication stress (PMID:15331764, PMID:18086888, PMID:27427983). It assembles a stable NSE1–NSE3–NSE4 subcomplex through conserved winged-helix domain contacts—N-terminal WH residues bind NSE1 and a C-terminal hydrophobic pocket binds the central helical NMBD region of the NSE4 kleisin—and this subcomplex bridges the SMC5 and SMC6 head domains (PMID:15601840, PMID:21364888, PMID:22536443, PMID:33676928). Within this module NSE3 contributes basic residues that directly bind double-stranded DNA, clamping the duplex from above and loading SMC5/6 stably onto chromatin; disrupting this DNA-binding surface reduces chromatin association and causes hypersensitivity to genotoxic agents and elevated chromosomal rearrangements (PMID:26446992, PMID:35648833, PMID:33860765). NSE3 is required for integrity of the whole complex, as its loss destabilizes and degrades partner subunits, and biallelic missense mutations that disrupt NSE1–NSE3–NSE4 assembly cause a chromosome-breakage syndrome with T- and B-cell immunodeficiency (PMID:18086888, PMID:27427983, PMID:33676928). NSE3 is itself sumoylated by the NSE2 ligase and ubiquitinated, and together with NSE4 it stimulates the ubiquitin E3 ligase activity of NSE1 (PMID:15601841, PMID:15601840, PMID:18086888, PMID:35011726). Beyond core complex function, NSMCE3 contributes to SMC5/6-mediated restriction of viral genomes, repressing HPV-31 replication and, as an NSMCE1/NSMCE3 subcomplex, inhibiting HBV transcription through ubiquitin-independent proteasomal degradation of HBx (PMID:32992873, PMID:40512786, PMID:41825673).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 2005 High

    Established that NSE3 is an integral subunit of the SMC5/6 complex and defined its immediate molecular neighborhood as a stable NSE1-NSE3-NSE4 subcomplex, anchoring later mechanistic work.

    Evidence Biochemical purification of Smc5-6 from S. pombe with interaction mapping and in vitro sumoylation assay

    PMID:15601840 PMID:15601841

    Open questions at the time
    • Did not establish the DNA-binding or enzymatic activity of the subcomplex
    • Functional consequence of NSE3 sumoylation undefined
  2. 2004 High

    Placed NSE3 functionally in genome maintenance by showing it is essential for chromosome segregation, DNA damage resistance, and acts in the homologous recombination pathway.

    Evidence Genetic screen, co-purification, and epistasis with rhp51/rad51 plus genotoxin sensitivity assays in S. pombe

    PMID:15331764

    Open questions at the time
    • Molecular activity of NSE3 within HR not defined
    • No structural basis for essentiality
  3. 2006 Medium

    Resolved how the subcomplex engages the SMC ATPase heads, showing the NSE1-NSE3-NSE4 and NSE5-NSE6 modules dock at distinct sites to bridge the Smc5/6 heads.

    Evidence In vitro interaction assays, domain mapping, and structural prediction

    PMID:17005570

    Open questions at the time
    • Architecture inferred from mapping/prediction rather than a solved structure
    • Single lab
  4. 2007 High

    Demonstrated that the yeast findings extend to the human complex and that NSE3 is required for stability of the entire SMC5/6 complex, since its depletion degrades partner subunits and sensitizes cells to DNA damage.

    Evidence Reciprocal Co-IP, siRNA depletion, MMS sensitivity assays, and detection of sumoylation/ubiquitination of human NSMCE3

    PMID:18086888

    Open questions at the time
    • Functional roles of NSE3 sumoylation and ubiquitination not dissected
    • Mechanism of subunit destabilization upon NSE3 loss unresolved
  5. 2011 High

    Mapped the residue-level interaction surfaces by which NSE3 binds NSE1 (N-terminal WH/A domain) and NSE4 (C-terminal WH/B hydrophobic surface), conserved in humans.

    Evidence Site-directed mutagenesis, yeast two-hybrid, Co-IP, modelling, and reporter assays

    PMID:21364888

    Open questions at the time
    • Did not address DNA binding
    • SF1 co-activation role of MAGEG1 not connected to SMC5/6 function
  6. 2012 Medium

    Defined the reciprocal binding determinant on the kleisin, identifying the Nse3/MAGE-binding domain (NMBD) of NSE4 that inserts into the conserved NSE3 hydrophobic pocket.

    Evidence Mutagenesis, yeast two-hybrid, PEPSCAN ELISA, and molecular docking/dynamics

    PMID:22536443

    Open questions at the time
    • Binding mode supported by modeling rather than a co-crystal
    • Single lab
  7. 2015 High

    Identified a core biochemical activity of NSE3—sequence-independent double-stranded DNA binding via basic residues—and linked it to chromatin loading of SMC5/6, converting NSE3 from a structural subunit to an active DNA-engaging element.

    Evidence In vitro DNA binding, mutagenesis, S. pombe genome integration of mutants, and ChIP; complemented by KITE/winged-helix structural classification

    PMID:26446992 PMID:26585514

    Open questions at the time
    • Spatial arrangement of DNA on the subcomplex not resolved at this stage
    • Relationship between DNA binding and HR repair function indirect
  8. 2016 High

    Connected NSE3 to human disease by showing biallelic missense mutations destabilize the SMC5/6 complex and cause a chromosome-breakage/immunodeficiency syndrome, validating NSE3-dependent complex integrity in patients.

    Evidence Whole exome sequencing, Co-IP interaction-disruption tests, cytogenetics, survival and HR assays in patient cells; plus telomeric roles defined in S. cerevisiae

    PMID:27427983 PMID:27564449

    Open questions at the time
    • Tissue-specific basis of immunodeficiency not explained
    • Telomere clustering role established only in budding yeast
  9. 2021 High

    Provided high-resolution structural and live-cell evidence for how NSE3 organizes the subcomplex and uses dsDNA binding to retain SMC5/6 on chromatin, including a structural rationale for disease mutations dislodging NSE4.

    Evidence 1.7 Å Xenopus Nse1-Nse3-Nse4 crystal structure, crosslinking-MS/EM of human subcomplex, and single-molecule tracking of nse3 separation-of-function mutants in fission yeast

    PMID:32389690 PMID:33676928 PMID:33860765

    Open questions at the time
    • dsDNA vs ssDNA contributions resolved functionally but not fully structurally
    • Human in-cell dynamics not directly measured
  10. 2022 Medium

    Captured NSE3 in the act of clamping DNA within the assembled Smc5/6 complex and established that NSE3 stimulates the partner NSE1 ubiquitin E3 ligase, defining both its DNA-engaging and enzyme-regulatory roles.

    Evidence 3.8 Å cryo-EM of DNA-bound Smc5/6 with crosslinking-MS and in vivo mutation analysis; in vitro ubiquitination assays with purified Nse1/Nse3/Nse4

    PMID:35011726 PMID:35648833

    Open questions at the time
    • Physiological ubiquitination substrate of the NSE1 ligase not identified
    • Coupling of DNA clamping to ATPase cycle unresolved
  11. 2026 Medium

    Extended NSE3 function to antiviral genome restriction, showing SMC5/6-NSE3 represses HPV-31 and that an NSMCE1/NSMCE3 subcomplex degrades HBx via a ubiquitin-independent proteasomal route to inhibit HBV.

    Evidence siRNA depletion with viral replication/transcription assays (HPV-31); cryo-EM of HBx-DDB1-NSE3, cell-free transcription, cycloheximide chase, proteasome inhibitor and 20S Co-IP experiments (HBV)

    PMID:32992873 PMID:40512786 PMID:41825673

    Open questions at the time
    • Mechanism linking 20S proteasome recruitment to ubiquitin-independent HBx degradation not fully detailed
    • Generality of antiviral restriction across virus families unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NSE3 DNA clamping is mechanically coupled to the SMC5/6 ATPase cycle and to recruitment of the NSE1 ligase toward physiological substrates remains unresolved.
  • No identified physiological NSE1 ubiquitination substrate
  • ATP-dependent loading mechanism in humans not reconstituted
  • Functional meaning of NSE3 sumoylation/ubiquitination unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 3 GO:0005198 structural molecule activity 2 GO:0060090 molecular adaptor activity 2 GO:0098772 molecular function regulator activity 1
Localization
GO:0005634 nucleus 2 GO:0005694 chromosome 2
Pathway
R-HSA-1643685 Disease 3 R-HSA-73894 DNA Repair 2 R-HSA-1640170 Cell Cycle 1
Partners
NSMCE1NSMCE2NSMCE4ASMC5SMC6
Complex memberships
NSE1-NSE3-NSE4 subcomplexSMC5/6 complex

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2005 NSE3 (Nse3) is a subunit of the Smc5-6 complex and forms a subcomplex with Nse1 and Nse4 (Nse1-Nse3-Rad62 in S. pombe). The Nse2 SUMO ligase sumoylates Nse3 in vitro in an Nse2-dependent manner. Biochemical purification of Smc5-6 complex from S. pombe; in vitro sumoylation assay Molecular and cellular biology High 15601840 15601841
2004 NSE3 (Nse3) is an essential nuclear protein required for normal mitotic chromosome segregation, DNA damage resistance, and meiosis. Epistasis with Rhp51 (Rad51) places Nse3 in the homologous recombination repair pathway. Genetic screen, biochemical co-purification, epistasis analysis with rhp51 mutants, sensitivity assays to genotoxic agents Molecular biology of the cell High 15331764
2005 NSE3 is a component of the Nse1-Nse3-Nse4 subcomplex within the Smc5-6 complex. This subcomplex also bridges the head domains of Smc5 and Smc6. Biochemical purification of Smc5-6 from S. pombe, identification of subcomplexes by interaction mapping Molecular and cellular biology High 15601840
2006 NSE3, as part of the Nse1-Nse3-Nse4 subcomplex, bridges the head domains of Smc5 and Smc6 in addition to the kleisin Nse4. The Nse1-Nse3-Nse4 and Nse5-Nse6 subcomplexes bind to the Smc5-Smc6 heads at different sites. In vitro protein interaction assays, structural predictions, domain mapping The Journal of biological chemistry Medium 17005570
2007 The human NSMCE3/MAGEG1 protein is a bona fide component of the human SMC5-6 complex. Depletion of NSMCE3 leads to degradation of the other SMC5-6 subunits and sensitivity to methyl methanesulfonate. NSMCE3 is modified by sumoylation and ubiquitination. Co-immunoprecipitation, siRNA depletion, sensitivity assays, detection of post-translational modifications Molecular and cellular biology High 18086888
2003 MAGE-G1 (NSMCE3 ortholog/alias) interacts with the transcription factor E2F1 via its transactivation domain, represses E2F1-dependent transcription, and antagonizes E2F1-induced apoptosis. MAGE-G1 also interacts with the p75 neurotrophin receptor. Co-immunoprecipitation, colony formation assay, BrdU incorporation, transcriptional reporter assay in N1E-115 cells The Journal of biological chemistry Medium 14593116
2011 A conserved hydrophobic surface on the C-terminal (WH/B) domain of NSE3/MAGEG1 mediates interaction with NSE4, and N-terminal (WH/A) domain residues are essential for interaction with NSE1. These interactions are conserved in human orthologs. MAGEG1 interaction with NSE4b results in transcriptional co-activation of the nuclear receptor SF1. Site-directed mutagenesis, yeast two-hybrid, Co-IP, molecular modelling, transcriptional reporter assay PloS one High 21364888
2015 NSE3, as part of the NSE1/NSE3/NSE4 subcomplex, binds double-stranded DNA without sequence preference. Mutations of key basic residues in the NSE3 DNA-binding surface reduce DNA binding in vitro and cause cell death or hypersensitivity to DNA-damaging agents in vivo. A hypomorphic nse3 DNA-binding mutant shows reduced association of SMC5/6 with chromatin by ChIP. In vitro DNA binding assays, site-directed mutagenesis, S. pombe genome integration of mutants, chromatin immunoprecipitation (ChIP) Nucleic acids research High 26446992
2015 NSE3 is structurally related to KITE (kleisin interacting tandem winged-helix elements) proteins and forms a heterodimer via its N-terminal WH domain, associating with the central part of the NSE4 kleisin subunit. In placental mammals, NSE3 gave rise to >60 MAGE-related KITE proteins. Structural similarity analysis, evolutionary bioinformatics, cross-species comparison Structure Medium 26585514
2016 Biallelic missense mutations in NSMCE3 disrupt interactions within the SMC5/6 complex, leading to destabilization of the complex. Patient cells show chromosome rearrangements, micronuclei, sensitivity to replication stress and DNA damage, and defective homologous recombination, causing a chromosome breakage syndrome with T and B cell immunodeficiency. Whole exome sequencing, Co-IP to test interaction disruption, cytogenetics, cell survival assays, HR assay in patient-derived cells The Journal of clinical investigation High 27427983
2016 NSE3 (Nse3 in S. cerevisiae) is required for Smc5/6 localization to telomeres. The temperature-sensitive nse3-1 mutant is defective in Smc5/6 telomere association, leading to defects in telomere clustering, Sir4 dispersion, and loss of transcriptional repression of sub-telomeric genes and TERRA. Nse3 physically interacts with the Rap1-binding factors Rif2 and Sir4. ChIP, co-immunoprecipitation, genetic interaction analysis, telomere length measurement PLoS genetics Medium 27564449
2012 A conserved Nse3/MAGE-binding domain (NMBD) within Nse4/EID proteins mediates binding to NSE3/MAGE proteins. The central helical region of NSE4/EID binds into the conserved hydrophobic pocket of MAGE proteins, with structure modeling supporting this interaction mode. Site-directed mutagenesis, yeast two-hybrid, PEPSCAN ELISA, molecular docking/dynamics simulation PloS one Medium 22536443
2022 Cryo-EM structure of DNA-bound Smc5/6 (including Nse1-3-4 subcomplex) at 3.8 Å shows NSE3 secures the DNA double helix from above within a clamp structure. NSE3 contributes positively charged residues to the DNA-binding inner surface. Mutational data confirm distinct DNA-binding contributions of NSE3 to chromatin association and cell fitness. Cryo-EM structure determination, crosslinking mass spectrometry, in vivo mutational analysis Proceedings of the National Academy of Sciences of the United States of America High 35648833
2021 Crystal structure of Xenopus laevis Nse1-Nse3-Nse4 subcomplex at 1.7 Å resolution shows Nse1-Nse3 dimer adopts a closed conformation forming three interfaces with Nse4, forcing Nse4 into a Z-shaped conformation. Disease-causing NSMCE3 mutations (lung disease/immunodeficiency syndrome) could dislodge Nse4 from the Nse1-Nse3 complex based on structural analysis. X-ray crystallography at 1.7 Å, DNA binding assays, mutational analysis Journal of molecular biology High 33676928
2021 NSE3 double-stranded DNA binding activity is critical for stable chromatin association of Smc5/6 in live cells. Disrupting Nse3 dsDNA binding reduces chromatin association, whereas disrupting ssDNA binding at the hinge does not prevent chromatin association but leads to elevated gross chromosomal rearrangements during replication restart. Single-molecule tracking in live fission yeast, defined point mutants in nse3 eLife High 33860765
2020 NSE3 depletion increases HPV-31 viral replication and transcription in keratinocytes maintaining episomal HPV-31, demonstrating that SMC5/6 (including NSE3) represses the HPV-31 replicative program. siRNA depletion of NSE3, viral replication and transcription assays, Co-IP Pathogens Medium 32992873
2020 The human NSE1-NSE3 heterodimer and NSE1-NSE3-NSE4 subcomplex architecture was determined by crosslinking-MS. NSE3 (hNSE3/MAGEG1) is positioned near the SMC head domains in the rod-like Smc5/6 complex. Crosslinking mass spectrometry, electron microscopy Journal of molecular biology Medium 32389690
2022 NSE3 (Nse3) stimulates the ubiquitin E3 ligase activity of NSE1 within the Smc5/6 complex. The Nse1 ligase activity is stimulated by Nse3 and Nse4 together using purified S. pombe proteins. In vitro ubiquitination assay with purified proteins, Nse1/Nse3/Nse4 combinations Cells Medium 35011726
2017 MAGE-G1 (NSMCE3) interacts with FSCN1 (fascin) and vimentin (VIME) during retinoic acid-induced neuronal differentiation of P19 cells, validated by GST pulldown and Co-IP. SILAC-based quantitative proteomics, GST pulldown, co-immunoprecipitation Scientific reports Low 28374796
2016 Crystal structures of MAGE-A3 and MAGE-A4 reveal a terminal peptide bound in a deep cleft between two tandem-arranged winged helix domains. Comparison with an effector-bound MAGE-G1 structure suggests a major conformational rearrangement is required for binding, implying allosteric regulation of effector binding in MAGE-G1/NSMCE3. X-ray crystallography of MAGE-A3 and MAGE-A4; structural comparison with MAGE-G1 PloS one Low 26910052
2023 C. elegans MAGE-1 (NSE3 ortholog) directly interacts with NSE-1 and NSE-4. Loss of mage-1/nse-3 reduces NSE-1 protein levels and causes NSE-1 mislocalization from nucleus to cytoplasm, indicating MAGE-1/NSE3 is essential for NSE-1 stability and proper SMC-5/6 complex function. Co-immunoprecipitation, GFP-tagged localization studies, genetic knockouts in C. elegans Genetics Medium 37579186
2025 The HBx-DDB1 complex directly and simultaneously interacts with NSE3, a component of the SMC5/6 complex, as revealed by cryo-EM structure and biochemical analysis. Cryo-EM structure of HBx-DDB1 complex; biochemical interaction analysis Proceedings of the National Academy of Sciences of the United States of America Medium 40512786
2026 The NSMCE1/NSMCE3 subcomplex is sufficient to inhibit HBV transcription in vitro and in cell-based assays. NSMCE1/3 promotes degradation of HBx via a ubiquitin-independent proteasomal mechanism; NSMCE1/3 interacts with the 20S proteasome but does not stimulate HBx ubiquitination. Knockdown of NSMCE3 promotes HBV proliferation. Cell-free transcription assay with purified proteins, RT-PCR, cycloheximide chase, proteasome inhibitor experiments, ubiquitination assay, Co-IP with 20S proteasome, siRNA knockdown International journal of biological macromolecules Medium 41825673

Source papers

Stage 0 corpus · 46 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Nse2, a component of the Smc5-6 complex, is a SUMO ligase required for the response to DNA damage. Molecular and cellular biology 196 15601841
2005 Composition and architecture of the Schizosaccharomyces pombe Rad18 (Smc5-6) complex. Molecular and cellular biology 98 15601840
2004 Nse1, Nse2, and a novel subunit of the Smc5-Smc6 complex, Nse3, play a crucial role in meiosis. Molecular biology of the cell 98 15331764
2015 Kite Proteins: a Superfamily of SMC/Kleisin Partners Conserved Across Bacteria, Archaea, and Eukaryotes. Structure (London, England : 1993) 92 26585514
2006 The Smc5-Smc6 DNA repair complex. bridging of the Smc5-Smc6 heads by the KLEISIN, Nse4, and non-Kleisin subunits. The Journal of biological chemistry 91 17005570
2003 Necdin-related MAGE proteins differentially interact with the E2F1 transcription factor and the p75 neurotrophin receptor. The Journal of biological chemistry 81 14593116
2007 Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex. Molecular and cellular biology 80 18086888
2016 Destabilized SMC5/6 complex leads to chromosome breakage syndrome with severe lung disease. The Journal of clinical investigation 64 27427983
2015 Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA. Nucleic acids research 64 26446992
2002 Allele-specific expression analysis by RNA-FISH demonstrates preferential maternal expression of UBE3A and imprint maintenance within 15q11- q13 duplications. Human molecular genetics 57 12095913
2008 Nse1 RING-like domain supports functions of the Smc5-Smc6 holocomplex in genome stability. Molecular biology of the cell 54 18667531
2011 Interactions between the Nse3 and Nse4 components of the SMC5-6 complex identify evolutionarily conserved interactions between MAGE and EID Families. PloS one 47 21364888
2022 Cryo-EM structure of DNA-bound Smc5/6 reveals DNA clamping enabled by multi-subunit conformational changes. Proceedings of the National Academy of Sciences of the United States of America 43 35648833
2015 The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex. Cell cycle (Georgetown, Tex.) 40 25590999
2017 Rifamorpholines A-E, potential antibiotics from locust-associated actinobacteria Amycolatopsis sp. Hca4. Organic & biomolecular chemistry 37 28422262
2020 Molecular Insights into the Architecture of the Human SMC5/6 Complex. Journal of molecular biology 32 32389690
2016 Structures of Two Melanoma-Associated Antigens Suggest Allosteric Regulation of Effector Binding. PloS one 32 26910052
2007 Single mage gene in the chicken genome encodes CMage, a protein with functional similarities to mammalian type II Mage proteins. Physiological genomics 32 17374844
2001 A necdin/MAGE-like gene in the chromosome 15 autism susceptibility region: expression, imprinting, and mapping of the human and mouse orthologues. BMC genetics 31 11782285
2017 Amycolamycins A and B, Two Enediyne-Derived Compounds from a Locust-Associated Actinomycete. Organic letters 30 29090939
2012 Analysis of the Nse3/MAGE-binding domain of the Nse4/EID family proteins. PloS one 30 22536443
2016 Smc5/6 Is a Telomere-Associated Complex that Regulates Sir4 Binding and TPE. PLoS genetics 28 27564449
2021 Live-cell single-molecule tracking highlights requirements for stable Smc5/6 chromatin association in vivo. eLife 26 33860765
2020 The SMC5/6 Complex Represses the Replicative Program of High-Risk Human Papillomavirus Type 31. Pathogens (Basel, Switzerland) 22 32992873
2013 The Smc5/Smc6/MAGE complex confers resistance to caffeine and genotoxic stress in Drosophila melanogaster. PloS one 17 23555814
2021 Structure Basis for Shaping the Nse4 Protein by the Nse1 and Nse3 Dimer within the Smc5/6 Complex. Journal of molecular biology 16 33676928
2017 Molecular evolution of type II MAGE genes from ancestral MAGED2 gene and their phylogenetic resolution of basal mammalian clades. Mammalian genome : official journal of the International Mammalian Genome Society 15 28516231
2017 High resolution crystal structures of Clostridium botulinum neurotoxin A3 and A4 binding domains. Journal of structural biology 15 29288126
2014 Necdin promotes ubiquitin-dependent degradation of PIAS1 SUMO E3 ligase. PloS one 15 24911587
2022 Role of Nse1 Subunit of SMC5/6 Complex as a Ubiquitin Ligase. Cells 13 35011726
2017 Interaction of the Saccharomyces cerevisiae RING-domain protein Nse1 with Nse3 and the Smc5/6 complex is required for chromosome replication and stability. Current genetics 9 29119272
2021 Kleisin NSE4 of the SMC5/6 complex is necessary for DNA double strand break repair, but not for recovery from DNA damage in Physcomitrella (Physcomitrium patens). Plant molecular biology 8 33550456
2025 Structural basis of the hepatitis B virus X protein in complex with DDB1. Proceedings of the National Academy of Sciences of the United States of America 6 40512786
2023 The SAGA histone acetyltransferase module targets SMC5/6 to specific genes. Epigenetics & chromatin 6 36793083
2024 Crucial role of the NSE1 RING domain in Smc5/6 stability and FANCM-independent fork progression. Cellular and molecular life sciences : CMLS 5 38847937
2022 Crystal Structures of Botulinum Neurotoxin Subtypes A4 and A5 Cell Binding Domains in Complex with Receptor Ganglioside. Toxins 5 35202156
2015 Nse1 and Nse4, subunits of the Smc5-Smc6 complex, are involved in Dictyostelium development upon starvation. Development, growth & differentiation 5 26036668
2025 AAVs targeting human carbonic anhydrase IV enhance gene delivery to the brain. Cell reports 4 41175373
2024 Enhancing Surface Modification and Carrier Extraction in Inverted Perovskite Solar Cells via Self-Assembled Monolayers. Nanomaterials (Basel, Switzerland) 4 38276732
2023 Botulinum Neurotoxin A4 Has a 1000-Fold Reduced Potency Due to Three Single Amino Acid Alterations in the Protein Receptor Binding Domain. International journal of molecular sciences 4 36982762
2022 Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans. International journal of molecular sciences 4 35806213
2021 New insights in phenotype and treatment of lung disease immuno-deficiency and chromosome breakage syndrome (LICS). Orphanet journal of rare diseases 4 33741030
2023 Caenorhabditis elegans NSE3 homolog (MAGE-1) is involved in genome stability and acts in inter-sister recombination during meiosis. Genetics 2 37579186
2017 Identification of Novel MAGE-G1-Interacting Partners in Retinoic Acid-Induced P19 Neuronal Differentiation Using SILAC-Based Proteomics. Scientific reports 2 28374796
2026 Mechanism insights into the role of Smc5/6 in HBV inhibition. International journal of biological macromolecules 0 41825673
2025 EBV-associated smooth muscle tumour: a clinicopathological and genetic study of nine cases revealing heterogeneous immune statuses and novel pathogenic mutations. Histopathology 0 40955557

Missed literature

Know a paper Affinage missed for NSMCE3? Flag it for the maintainers and the community.

No submissions yet.