Affinage

NINL

Ninein-like protein · UniProt Q9Y2I6

Length
1382 aa
Mass
156.3 kDa
Annotated
2026-06-10
100 papers in source corpus 13 papers cited in narrative 13 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NINL (ninein-like protein, Nlp) is a centrosomal organizer of microtubule arrays whose cell-cycle-regulated phosphorylation couples microtubule nucleation to mitotic progression (PMID:12852856, PMID:15684383). During interphase it binds components of the gamma-tubulin ring complex to stimulate microtubule nucleation, and its centrosomal targeting depends on the dynein-dynactin motor (PMID:12852856, PMID:16254247). At the G2/M transition a phosphorylation cascade displaces it from the centrosome: Cdc2/cyclin B1 phosphorylates Ser185 to prime recognition by Plk1, Nek2 further primes Plk1 phosphorylation, and Plk1 then disrupts both the gamma-tubulin and dynactin interactions to release NINL from the centrosome (PMID:16254247, PMID:15684383, PMID:20890132). NINL is independently recruited to the midbody by Aurora B, which phosphorylates it to drive completion of cytokinesis; loss of NINL or its phosphosites produces aberrant spindles, aborted cytokinesis, aneuploidy, and chromosomal instability (PMID:19509300, PMID:20864540). Beyond the centrosome NINL operates as a dynein-associated adaptor for membrane trafficking: it partners with DZANK1 to assemble cytoplasmic dynein 1 and with the ciliopathy protein CC2D2A, RAB8 and MICAL3 at the ciliary base, and its loss in zebrafish causes photoreceptor outer-segment defects, opsin/Ush2a mislocalization, vesicle accumulation and impaired retrograde transport (PMID:26485645, PMID:26485514). It further acts as a vesicle-trafficking platform, bridging Rab7 and FYCO1 to accelerate autophagic flux and binding SEC31A and Rab1B to support COPII-mediated ER-to-Golgi cargo transport, with deficiency triggering ER stress and the PERK-eIF2alpha UPR (PMID:33859171, PMID:38904019). NINL additionally translocates to the nucleus after UV damage to scaffold the XPA-ERCC1 nucleotide excision repair factors, and is a positively selected component of the antiviral interferon response that is cleaved by diverse picornaviral and coronaviral proteases (PMID:26805762, PMID:36222652).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 2003 High

    Established NINL's core molecular function by showing it binds the gamma-TuRC to nucleate microtubules and is regulated by Plk1, explaining how microtubule organization is reset at mitotic onset.

    Evidence Co-IP, in vitro kinase assay, and phospho-mutant overexpression in cultured cells

    PMID:12852856

    Open questions at the time
    • Did not define upstream priming kinases
    • Structural basis of gamma-tubulin binding not resolved
  2. 2005 High

    Connected NINL's centrosomal targeting to the dynein-dynactin motor and showed Plk1 phosphorylation severs this link, providing a mechanism coupling motor-dependent localization to the cell cycle.

    Evidence Reciprocal Co-IP, dominant-negative dynactin, and in vitro kinase assay

    PMID:16254247

    Open questions at the time
    • Which dynactin subunit mediates the interaction not pinpointed
  3. 2005 High

    Identified Nek2 as a second NINL kinase that primes Plk1 phosphorylation, ordering the G2/M phosphorylation cascade controlling centrosomal release.

    Evidence In vitro kinase assays with kinase-active/inactive mutants and localization in cells

    PMID:15684383

    Open questions at the time
    • In vivo sequence of Nek2-then-Plk1 events not directly demonstrated
  4. 2009 Medium

    Linked NINL to genome stability by showing BRCA1 maintains its centrosomal localization and that NINL depletion causes spindle defects and aneuploidy.

    Evidence Co-IP, colocalization, siRNA knockdown in BRCA1-mutant cell lines

    PMID:19509300

    Open questions at the time
    • BRCA1-Plk1-NINL regulatory chain inferred, not directly mapped
    • Single-lab finding
  5. 2010 Medium

    Mapped Cdc2/cyclin B1 phosphosites (Ser185, Ser589) defining how NINL is primed for Plk1 recognition and how its stability is controlled at mitosis.

    Evidence In vitro kinase assay, site-directed mutagenesis, immunofluorescence

    PMID:20890132

    Open questions at the time
    • Degradation machinery acting on Ser589-phosphorylated NINL not identified
  6. 2010 High

    Extended NINL function into cytokinesis, showing Aurora B recruits and phosphorylates it at the midbody to complete cell division.

    Evidence Co-IP, in vitro kinase assay, mutagenesis, and siRNA knockdown with cytokinesis readout

    PMID:20864540

    Open questions at the time
    • Midbody binding partners of NINL beyond Aurora B unresolved
  7. 2010 Medium

    Demonstrated that NINL dysregulation is oncogenic, with overexpression driving transformation, centrosome amplification, and spontaneous tumors in mice.

    Evidence Transformation assay, transgenic mouse model, MEF centrosome analysis

    PMID:20093778

    Open questions at the time
    • Mechanistic link between overexpression and centrosome amplification not dissected
    • Single-lab finding
  8. 2015 High

    Revealed a ciliary/photoreceptor trafficking role by placing NINL with CC2D2A, RAB8 and MICAL3 at the cilium base and demonstrating a genetic interaction with cc2d2a in vivo.

    Evidence Co-IP, zebrafish morpholino knockdown, genetic epistasis, interactome proteomics

    PMID:26485645

    Open questions at the time
    • Direct vs indirect nature of NINL-MICAL3 interaction not separated
  9. 2015 High

    Defined the NINL-DZANK1 module as a dynein-1 assembly adaptor, showing synergistic photoreceptor and retrograde transport defects upon loss.

    Evidence Yeast two-hybrid, Co-IP, zebrafish mutant/morphant phenotypes, retrograde melanosome transport assay

    PMID:26485514

    Open questions at the time
    • Stoichiometry of NINL-DZANK1-dynein assembly not resolved
  10. 2016 Medium

    Uncovered a nuclear, UV-inducible role in nucleotide excision repair, with NINL scaffolding XPA-ERCC1 to promote repair.

    Evidence Co-IP, nuclear fractionation, NER activity assay, deletion mapping, siRNA knockdown

    PMID:26805762

    Open questions at the time
    • How the centrosomal protein relocalizes and is retained in the nucleus unclear
    • Single-lab finding
  11. 2021 Medium

    Identified NINL as an autophagy enhancer bridging Rab7 and FYCO1 to accelerate autophagic flux, with knockout promoting carcinogen-induced liver cancer.

    Evidence Co-IP, colocalization, autophagy flux assay, mouse knockout/carcinogen model

    PMID:33859171

    Open questions at the time
    • Whether the Rab7-FYCO1 bridging requires dynein not addressed
    • Single-lab finding
  12. 2022 High

    Established NINL as an antiviral innate immune factor under positive selection that is directly cleaved by picornaviral and coronaviral proteases.

    Evidence Knockout cell interferon and viral replication assays, protease cleavage assays, evolutionary analysis

    PMID:36222652

    Open questions at the time
    • Molecular step in the interferon pathway NINL acts on not defined
  13. 2024 Medium

    Defined NINL as an ER-to-Golgi cargo adaptor binding SEC31A and Rab1B, with deficiency causing ER stress, UPR activation, and B cell lymphoma in mice.

    Evidence Co-IP with SEC31A/Rab1B, knockdown/knockout cells, ER stress assays, mouse knockout

    PMID:38904019

    Open questions at the time
    • Cargo selectivity mechanism not fully resolved
    • Single-lab finding

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NINL's many roles—centrosomal microtubule nucleation, dynein-adaptor trafficking, autophagy, COPII transport, NER, and antiviral defense—are partitioned by isoform, localization, or context remains unresolved.
  • No unifying model integrating centrosomal and trafficking/immune functions
  • Domain requirements for each function not comparatively mapped

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 3 GO:0008092 cytoskeletal protein binding 2
Localization
GO:0005815 microtubule organizing center 3 GO:0005929 cilium 2 GO:0031410 cytoplasmic vesicle 2 GO:0005634 nucleus 1
Pathway
R-HSA-1640170 Cell Cycle 2 R-HSA-5653656 Vesicle-mediated transport 2 R-HSA-168256 Immune System 1 R-HSA-73894 DNA Repair 1 R-HSA-9612973 Autophagy 1
Partners
CC2D2ADZANK1FYCO1MICAL3RAB1BRAB7SEC31AXPA
Complex memberships
cytoplasmic dynein 1 complexgamma-tubulin ring complex (gamma-TuRC)

Evidence

Reading pass · 13 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 NINL (Nlp/ninein-like protein) interacts with two components of the gamma-tubulin ring complex (gamma-TuRC) and stimulates microtubule nucleation. Plk1 phosphorylates Nlp and disrupts both its centrosome association and its gamma-tubulin interaction. Overexpression of an Nlp mutant lacking Plk1 phosphorylation sites severely disturbs mitotic spindle formation, indicating Nlp plays a role in microtubule organization during interphase and is displaced from the centrosome by Plk1 at mitotic onset. Co-immunoprecipitation, in vitro kinase assay, overexpression/mutagenesis, immunofluorescence microscopy Developmental cell High 12852856
2005 Nlp and ninein interact with the dynein-dynactin motor complex, and dynactin is required for the targeting of Nlp to the centrosome. Phosphorylation of Nlp by Plk1 negatively regulates its association with dynactin, thereby providing a mechanism by which Plk1 coordinates changes in microtubule organization with cell cycle progression. Co-immunoprecipitation, overexpression/dominant-negative dynactin, immunofluorescence, in vitro kinase assay Journal of cell science High 16254247
2005 NINL (Nlp) is also a substrate of Nek2 kinase in addition to Plk1. X-Nlp (Xenopus homolog) is a mother-centriole-specific protein. Overexpression of active Nek2 or Plk1 causes premature displacement of Nlp from interphase centrosomes. Nek2 phosphorylation of Nlp does not disrupt its gamma-tubulin interaction, but active Nek2 stimulates subsequent Plk1 phosphorylation of Nlp in vitro, suggesting that Nek2 primes Nlp for Plk1 phosphorylation at the G2/M transition. In vitro kinase assay, immunofluorescence, overexpression of kinase-active/inactive mutants, cell fractionation Molecular and cellular biology High 15684383
2009 BRCA1 physically interacts and colocalizes with Nlp at the centrosome. Cells with BRCA1 mutations or BRCA1 silencing exhibit disrupted Nlp colocalization to centrosomes and enhanced Nlp degradation, likely through BRCA1-mediated suppression of Plk1. siRNA-mediated depletion of Nlp results in aberrant spindle formation, aborted chromosomal segregation, and aneuploidy. Co-immunoprecipitation, colocalization by immunofluorescence, siRNA knockdown, BRCA1 mutation cell lines The Journal of biological chemistry Medium 19509300
2010 Cdc2/cyclin B1 phosphorylates Nlp at Ser185 and Ser589. Ser185 phosphorylation is required for recognition of Nlp by Plk1, enabling Nlp departure from centrosomes at mitotic onset; Plk1 fails to dissociate an Nlp Ser185 mutant from the centrosome. Ser589 phosphorylation by Cdc2/cyclin B1 affects Nlp protein stability/degradation. Loss of these sites leads to multinucleated cells. In vitro kinase assay, site-directed mutagenesis, immunofluorescence, Western blot Cancer biology & therapy Medium 20890132
2010 Nlp localizes to the midbody during cytokinesis and is required for completion of cell division. Aurora B recruits Nlp to the midbody via direct physical interaction. Aurora B phosphorylates Nlp at Ser-185, Ser-448, and Ser-585; phosphorylation at Ser-448 and Ser-585 is required for Nlp association with Aurora B and midbody localization, while Ser-185 phosphorylation affects Nlp stability. Depletion of Nlp or disruption of phosphorylation sites causes aborted cytokinesis and chromosomal instability. Co-immunoprecipitation, in vitro kinase assay, site-directed mutagenesis, immunofluorescence, siRNA knockdown The Journal of biological chemistry High 20864540
2010 Overexpression of Nlp in NIH3T3 cells confers anchorage-independent growth and tumor formation in nude mice. Transgenic mice overexpressing Nlp develop spontaneous tumors and show centrosome amplification in MEFs, suggesting that Nlp overexpression mimics BRCA1 loss and contributes to genomic instability. Stable transfection/transformation assay, transgenic mouse model, immunohistochemistry, MEF centrosome analysis The Journal of clinical investigation Medium 20093778
2015 NINL physically interacts with the ciliopathy protein CC2D2A at the base of cilia. Knockdown of ninl in zebrafish leads to photoreceptor outer segment loss, mislocalization of opsins, vesicle accumulation, and altered Rab8a localization, resembling cc2d2a-/- phenotypes. Partial ninl knockdown in cc2d2a-/- embryos enhances the retinal phenotype, indicating a genetic interaction. The NINL-associated interactome includes MICAL3, a Rab8-interacting protein involved in vesicle docking and fusion. Co-immunoprecipitation (pulldown), zebrafish morpholino knockdown, genetic interaction/epistasis, immunofluorescence localization, proteomics interactome PLoS genetics High 26485645
2015 NINL interacts with DZANK1 and both proteins associate with complementary subunits of the cytoplasmic dynein 1 motor complex. Loss of Ninl, Dzank1, or both synergistically causes dysmorphic photoreceptor outer segments, accumulation of trans-Golgi-derived vesicles, mislocalization of Rhodopsin and Ush2a, and severely impaired retrograde melanosome transport in zebrafish, supporting a role for the NINL-DZANK1 module in assembly of the cytoplasmic dynein 1 complex. Yeast two-hybrid, co-immunoprecipitation, zebrafish morpholino/mutant phenotype analysis, immunofluorescence, retrograde transport assay PLoS genetics High 26485514
2016 Upon UVC irradiation, NINL (Nlp) translocates into the nucleus via its C-terminus (residues 1030–1382) and physically interacts with the NER factors XPA and ERCC1, enhancing their association. Nlp depletion impairs nucleotide excision repair (NER) activity and sensitizes cells to UV radiation. Co-immunoprecipitation, nuclear fractionation, NER activity assay, deletion mapping, immunofluorescence, siRNA knockdown Cancer letters Medium 26805762
2021 NINL (Nlp) colocalizes with autophagosomes and physically interacts with LC3 (autophagosome marker), Rab7, and FYCO1. Nlp enhances the Rab7–FYCO1 interaction, thereby accelerating autophagic flux and autophagolysosome formation. NLP-deficient mice treated with DMBA show increased liver cancer incidence associated with hepatic autophagic defects. Co-immunoprecipitation, immunofluorescence colocalization, autophagy flux assay, mouse knockout/carcinogen model Signal transduction and targeted therapy Medium 33859171
2022 NINL has evolved under recurrent positive selection, particularly in its carboxy-terminal cargo-binding region. NINL knockout cells exhibit an impaired interferon response, resulting in increased permissiveness to viral replication. Proteases encoded by diverse picornaviruses and coronaviruses cleave and disrupt NINL function in a host- and virus-specific manner, identifying NINL as a component of the antiviral innate immune response and a target of viral antagonism. NINL knockout cells (interferon response assay, viral replication assay), viral protease cleavage assay, evolutionary analysis (signatures of positive selection) eLife High 36222652
2024 NINL (Nlp) acts as an adaptor/platform for ER-to-Golgi vesicle trafficking by directly binding SEC31A (a COPII coat component) and Rab1B, facilitating selection and continuity of specific cargoes (including β-Catenin and STING) through COPII- and COPI-coated vesicle transition. Nlp deficiency causes vesicle budding failure, ER protein accumulation, ER stress, Golgi fragmentation, and activation of the PERK-eIF2α UPR pathway. Nlp-deficient mice develop spontaneous B cell lymphoma, linked to defective secretory protein trafficking in lymphocytes. Co-immunoprecipitation (with SEC31A and Rab1B), knockdown/knockout cells, ER stress assay (PERK-eIF2α), immunofluorescence (Golgi fragmentation), mouse knockout model International journal of biological sciences Medium 38904019

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2017 Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks. Nature 454 28489820
2015 An RLP23-SOBIR1-BAK1 complex mediates NLP-triggered immunity. Nature plants 357 27251392
2003 Polo-like kinase 1 regulates Nlp, a centrosome protein involved in microtubule nucleation. Developmental cell 216 12852856
2017 Interacting TCP and NLP transcription factors control plant responses to nitrate availability. Proceedings of the National Academy of Sciences of the United States of America 200 28202720
2021 The language of proteins: NLP, machine learning & protein sequences. Computational and structural biotechnology journal 196 33897979
2013 The nucleoplasmin homolog NLP mediates centromere clustering and anchoring to the nucleolus. Molecular cell 87 23562326
2005 Phosphorylation of Nlp by Plk1 negatively regulates its dynein-dynactin-dependent targeting to the centrosome. Journal of cell science 86 16254247
2005 Coordinate regulation of the mother centriole component nlp by nek2 and plk1 protein kinases. Molecular and cellular biology 81 15684383
2021 Different DNA-binding specificities of NLP and NIN transcription factors underlie nitrate-induced control of root nodulation. The Plant cell 78 33826745
2007 Impaired processing of FLP and NLP peptides in carboxypeptidase E (EGL-21)-deficient Caenorhabditis elegans as analyzed by mass spectrometry. Journal of neurochemistry 77 17564681
2012 The NLP toxin family in Phytophthora sojae includes rapidly evolving groups that lack necrosis-inducing activity. Molecular plant-microbe interactions : MPMI 75 22397404
2015 The Ciliopathy Protein CC2D2A Associates with NINL and Functions in RAB8-MICAL3-Regulated Vesicle Trafficking. PLoS genetics 71 26485645
2003 The crystal structure of Drosophila NLP-core provides insight into pentamer formation and histone binding. Structure (London, England : 1993) 68 12575937
2022 AttentionSiteDTI: an interpretable graph-based model for drug-target interaction prediction using NLP sentence-level relation classification. Briefings in bioinformatics 61 35817396
2002 Common and differential chemokine expression patterns in rs cells of NLP, EBV positive and negative classical Hodgkin lymphomas. International journal of cancer 57 12115499
2001 TARC, a CC chemokine, is frequently expressed in classic Hodgkin's lymphoma but not in NLP Hodgkin's lymphoma, T-cell-rich B-cell lymphoma, and most cases of anaplastic large cell lymphoma. The American journal of surgical pathology 53 11420464
2013 The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans. Nature communications 52 24301180
2014 Characterization of necrosis-inducing NLP proteins in Phytophthora capsici. BMC plant biology 47 24886309
2009 Life-span extension by dietary restriction is mediated by NLP-7 signaling and coelomocyte endocytosis in C. elegans. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 46 19783783
2007 Functional analysis of NLP genes from Botrytis elliptica. Molecular plant pathology 44 20507492
2020 Genome wide analysis of NLP transcription factors reveals their role in nitrogen stress tolerance of rice. Scientific reports 39 32523127
2021 COVID-19 SignSym: a fast adaptation of a general clinical NLP tool to identify and normalize COVID-19 signs and symptoms to OMOP common data model. Journal of the American Medical Informatics Association : JAMIA 34 33674830
2010 Centrosomal Nlp is an oncogenic protein that is gene-amplified in human tumors and causes spontaneous tumorigenesis in transgenic mice. The Journal of clinical investigation 33 20093778
2003 Kinase pathway database: an integrated protein-kinase and NLP-based protein-interaction resource. Genome research 32 12799355
2018 Genome-Wide Identification and Characterization of NODULE-INCEPTION-Like Protein (NLP) Family Genes in Brassica napus. International journal of molecular sciences 31 30072649
2002 Germline FAS gene mutation in a case of ALPS and NLP Hodgkin lymphoma. Blood 31 11830507
2010 NLP is a novel transcription regulator involved in VSG expression site control in Trypanosoma brucei. Nucleic acids research 29 21076155
2018 Neuropeptides encoded by nlp-49 modulate locomotion, arousal and egg-laying behaviours in Caenorhabditis elegans via the receptor SEB-3. Philosophical transactions of the Royal Society of London. Series B, Biological sciences 28 30201834
2014 Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization. Genes & development 27 25228646
2022 Identification of LINC00654-NINL Regulatory Axis in Diffuse Large B-Cell Lymphoma In Silico Analysis. Frontiers in oncology 26 35719990
2015 NINL and DZANK1 Co-function in Vesicle Transport and Are Essential for Photoreceptor Development in Zebrafish. PLoS genetics 25 26485514
2009 BRCA1 interaction of centrosomal protein Nlp is required for successful mitotic progression. The Journal of biological chemistry 25 19509300
2018 Identification and functional analysis of the NLP-encoding genes from the phytopathogenic oomycete Phytophthora capsici. Molecular genetics and genomics : MGG 24 29572661
2017 Inappropriate Expression of an NLP Effector in Colletotrichum orbiculare Impairs Infection on Cucurbitaceae Cultivars via Plant Recognition of the C-Terminal Region. Molecular plant-microbe interactions : MPMI 24 29059009
2021 Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1. Signal transduction and targeted therapy 23 33859171
1989 NLP-1: a DNA intercalating hypoxic cell radiosensitizer and cytotoxin. International journal of radiation oncology, biology, physics 23 2703383
2022 An oomycete NLP cytolysin forms transient small pores in lipid membranes. Science advances 22 35275729
2019 Identification and Functional Analysis of NLP-Encoding Genes from the Postharvest Pathogen Penicillium expansum. Microorganisms 21 31208074
2018 Phylogenetic, expression and functional characterizations of the maize NLP transcription factor family reveal a role in nitrate assimilation and signaling. Physiologia plantarum 21 29364528
2015 NLP-12 engages different UNC-13 proteins to potentiate tonic and evoked release. The Journal of neuroscience : the official journal of the Society for Neuroscience 21 25609620
2011 Expression of an oxalate decarboxylase impairs the necrotic effect induced by Nep1-like protein (NLP) of Moniliophthora perniciosa in transgenic tobacco. Molecular plant-microbe interactions : MPMI 21 21405988
2011 The role of centrosomal Nlp in the control of mitotic progression and tumourigenesis. British journal of cancer 20 21505454
2008 Increased expression of Nlp, a potential oncogene in ovarian cancer, and its implication in carcinogenesis. Gynecologic oncology 20 18538832
2022 CollagenTransformer: End-to-End Transformer Model to Predict Thermal Stability of Collagen Triple Helices Using an NLP Approach. ACS biomaterials science & engineering 19 36149671
2021 Genome-wide survey and expression analysis of NIN-like Protein (NLP) genes reveals its potential roles in the response to nitrate signaling in tomato. BMC plant biology 17 34301191
1989 Cloning and sequencing of an Escherichia coli gene, nlp, highly homologous to the ner genes of bacteriophages Mu and D108. Journal of bacteriology 17 2670911
2022 Antiviral function and viral antagonism of the rapidly evolving dynein activating adaptor NINL. eLife 15 36222652
2021 In silico analyses of neuropeptide-like protein (NLP) profiles in parasitic nematodes. International journal for parasitology 15 34450132
2009 Comparison of Caenorhabditis elegans NLP peptides with arthropod neuropeptides. Trends in parasitology 15 19269897
2020 RPamide neuropeptides NLP-22 and NLP-2 act through GnRH-like receptors to promote sleep and wakefulness in C. elegans. Scientific reports 14 32555288
2012 Overexpression of centrosomal protein Nlp confers breast carcinoma resistance to paclitaxel. Cancer biology & therapy 14 22353935
2024 Genome-wide identification and expression analysis of NIN-like protein (NLP) genes: Exploring their potential roles in nitrate response in tea plant (Camellia sinensis). Plant physiology and biochemistry : PPB 13 38199025
2023 Transcription factor module NLP-NIGT1 fine-tunes NITRATE TRANSPORTER2.1 expression. Plant physiology 13 37595050
2017 RNAi-mediated disruption of neuropeptide genes, nlp-3 and nlp-12, cause multiple behavioral defects in Meloidogyne incognita. Biochemical and biophysical research communications 13 28655614
2010 Aurora B interaction of centrosomal Nlp regulates cytokinesis. The Journal of biological chemistry 13 20864540
2010 Cdc2/cyclin B1 regulates centrosomal Nlp proteolysis and subcellular localization. Cancer biology & therapy 13 20890132
1999 Specific antibodies reactive with the 22-kilodalton major outer surface protein of Borrelia anserina Ni-NL protect chicks from infection. Infection and immunity 13 10225933
2021 Automated NLP Extraction of Clinical Rationale for Treatment Discontinuation in Breast Cancer. JCO clinical cancer informatics 12 33989016
2012 Non Linear Programming (NLP) formulation for quantitative modeling of protein signal transduction pathways. PloS one 12 23226239
1992 The Escherichia coli Mu/D108 phage ner homologue gene (nlp) is transcribed and evolutionarily conserved among the Enterobacteriaceae. Gene 12 1534062
2020 Machine learning and natural language processing (NLP) approach to predict early progression to first-line treatment in real-world hormone receptor-positive (HR+)/HER2-negative advanced breast cancer patients. European journal of cancer (Oxford, England : 1990) 11 33373867
2015 Blending water- and nutrient-source wastewaters for cost-effective cultivation of high lipid content microalgal species Micractinium inermum NLP-F014. Bioresource technology 9 26409109
2008 Estimating relative risks for common outcome using PROC NLP. Computer methods and programs in biomedicine 9 18291556
2024 Using meta-analysis and CNN-NLP to review and classify the medical literature for normal tissue complication probability in head and neck cancer. Radiation oncology (London, England) 8 38195582
2022 Stemphylium lycopersici Nep1-like Protein (NLP) Is a Key Virulence Factor in Tomato Gray Leaf Spot Disease. Journal of fungi (Basel, Switzerland) 8 35628773
2022 NLP-BCH-Ens: NLP-based intelligent computational model for discrimination of malaria parasite. Computers in biology and medicine 8 36049412
2024 An NLP-based technique to extract meaningful features from drug SMILES. iScience 7 38455979
2022 Triplication is the main evolutionary driving force of NLP transcription factor family in Chinese cabbage and related species. International journal of biological macromolecules 7 35051503
2020 COVID-19 SignSym: a fast adaptation of a general clinical NLP tool to identify and normalize COVID-19 signs and symptoms to OMOP common data model. ArXiv 7 32908948
2006 Developing an NLP and IR-based algorithm for analyzing gene-disease relationships. Methods of information in medicine 7 16685344
2024 Caenorhabditis elegans neuropeptide NLP-27 enhances neurodegeneration and paralysis in an opioid-like manner during fungal infection. iScience 6 38784855
2021 The CCCH-Type Zinc Finger Antiviral Protein Relieves Immunosuppression of T Cells Induced by Avian Leukosis Virus Subgroup J via the NLP-PKC-δ-NFAT Pathway. Journal of virology 6 34705559
2018 Mining protein phosphorylation information from biomedical literature using NLP parsing and Support Vector Machines. Computer methods and programs in biomedicine 6 29728247
2015 Synthesis and characterization of Her2-NLP peptide conjugates targeting circulating breast cancer cells: cellular uptake and localization by fluorescent microscopic imaging. Journal of fluorescence 6 25620472
2015 Mass Spectrometry of Single GABAergic Somatic Motorneurons Identifies a Novel Inhibitory Peptide, As-NLP-22, in the Nematode Ascaris suum. Journal of the American Society for Mass Spectrometry 6 26174364
2020 Triage of documents containing protein interactions affected by mutations using an NLP based machine learning approach. BMC genomics 5 33167858
2011 A pharmacological study of NLP-12 neuropeptide signaling in free-living and parasitic nematodes. Peptides 5 22019590
2023 Identification and expression characteristics of NLP (NIN-like protein) gene family in pepper (Capsicum annuum L.). Molecular biology reports 4 37358766
2022 Efficient E-Mail Spam Detection Strategy Using Genetic Decision Tree Processing with NLP Features. Computational intelligence and neuroscience 4 35371228
2021 Tuning Selective Transport of Biomolecules through Site-Mutated Nucleoporin-like Protein (NLP) Hydrogels. Biomacromolecules 4 33428378
2016 Mitotic regulator Nlp interacts with XPA/ERCC1 complexes and regulates nucleotide excision repair (NER) in response to UV radiation. Cancer letters 4 26805762
2024 Nlp-dependent ER-to-Golgi transport. International journal of biological sciences 3 38904019
2024 Temperature-dependent lifespan extension is achieved in miR-80-deleted Caenorhabditis elegans by NLP-45 to modulate endoplasmic reticulum unfolded protein responses. Aging cell 3 39323014
2023 Bioactive Peptide Recognition Based on NLP Pre-Train Algorithm. IEEE/ACM transactions on computational biology and bioinformatics 3 37815965
2022 Genome-Wide Analysis and Evolutionary History of the Necrosis- and Ethylene-Inducing Peptide 1-Like Protein (NLP) Superfamily Across the Dothideomycetes Class of Fungi. Current microbiology 3 36536087
2002 DNA-targeted 2-nitroimidazoles: studies of the influence of the phenanthridine-linked nitroimidazoles, 2-NLP-3 and 2-NLP-4, on DNA damage induced by ionizing radiation. Radiation research 3 12175307
2025 A novel NLP-based method and algorithm to discover RNA-binding protein (RBP) motifs, contexts, binding preferences, and interactions. bioRxiv : the preprint server for biology 2 39896518
2025 Hard label adversarial attack with high query efficiency against NLP models. Scientific reports 2 40102502
2025 Interdependence between SEB-3 receptor and NLP-49 peptides shifts across predator-induced defensive behavioral modes in Caenorhabditis elegans. eLife 2 40163376
2025 Nanolipoprotein particle (NLP) vaccine confers protection against Yersinia pestis aerosol challenge in a BALB/c mouse model. Frontiers in immunology 2 40642079
2025 Optimized BERT-based NLP outperforms zero-shot methods for automated symptom detection in clinical practice. Frontiers in digital health 2 41383383
2024 GeNLP: a web tool for NLP-based exploration and prediction of microbial gene function. Bioinformatics (Oxford, England) 2 38291951
2024 The Potential of Using Generative AI/NLP to Identify and Analyse Critical Incidents in a Critical Incident Reporting System (CIRS): A Feasibility Case-Control Study. Healthcare (Basel, Switzerland) 2 39408144
2022 The necrosis- and ethylene-inducing peptide 1-like protein (NLP) gene family of the plant pathogen Corynespora cassiicola. Current genetics 2 36098767
2025 An NLP-based method to mine gene and function relationships from published articles. Scientific reports 1 40033048
2024 New alleles of nlp-2 , nlp-22 , and nlp-23 demonstrate that they are dispensable for stress-induced sleep in C. elegans. microPublication biology 1 38371321
2024 Genome-Wide Identification of NLP Gene Families and Haplotype Analysis of SiNLP2 in Foxtail Millet (Setaria italica). International journal of molecular sciences 1 39684649
2024 Circular RNA NINL accelerates the malignant progression of cervical cancer. Discover oncology 1 39692846
2022 Nlp enhances biofilm formation by Yersinia pestis biovar microtus. Microbial pathogenesis 1 35760284
2022 RegenX: an NLP recommendation engine for neuroregeneration topics over time. Annals of eye science 1 36199680

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