Affinage

NINL

Ninein-like protein · UniProt Q9Y2I6

Length
1382 aa
Mass
156.3 kDa
Annotated
2026-04-29
100 papers in source corpus 13 papers cited in narrative 13 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NINL (ninein-like protein) is a multifunctional dynein-associated adaptor that couples microtubule organization, vesicle trafficking, and signaling across several cellular compartments. During interphase, NINL localizes to the centrosome, interacts with γ-tubulin ring complex components to stimulate microtubule nucleation, and is displaced at mitotic onset through sequential phosphorylation by Cdc2/cyclin B1, Nek2, and Plk1, which disrupts its dynein-dynactin interaction (PMID:12852856, PMID:16254247, PMID:15684383, PMID:20890132); Aurora B subsequently phosphorylates NINL and recruits it to the midbody for cytokinesis (PMID:20864540). NINL functions as a dynein activating adaptor essential for ciliary vesicle trafficking—interacting with CC2D2A and DZANK1 to facilitate dynein-1 complex assembly and photoreceptor outer segment biogenesis—and also mediates ER-to-Golgi transport by binding SEC31A and Rab1B, promotes autophagolysosome formation by enhancing Rab7–FYCO1 interaction, and participates in nucleotide excision repair through nuclear translocation and interaction with XPA and ERCC1 (PMID:26485645, PMID:26485514, PMID:38904019, PMID:33859171, PMID:26805762). NINL is a critical antiviral innate immune effector whose loss impairs interferon responses and whose cleavage by picornavirus and coronavirus proteases facilitates viral replication; its overexpression drives centrosome amplification and tumorigenesis (PMID:36222652, PMID:20093778).

Mechanistic history

Synthesis pass · year-by-year structured walk · 9 steps
  1. 2003 High

    Establishing NINL as a centrosomal microtubule-nucleation factor regulated by Plk1 answered how interphase centrosomal organization is dismantled at mitotic entry.

    Evidence Co-immunoprecipitation with γ-tubulin ring complex components, in vitro Plk1 kinase assay, phospho-mutant overexpression in human cells

    PMID:12852856

    Open questions at the time
    • Identity of the specific γ-TuRC subunits bound by NINL not fully resolved
    • Whether Plk1 phosphorylation is sufficient in vivo or requires priming kinases was unknown
  2. 2005 High

    Demonstrating that NINL reaches centrosomes via dynein-dynactin and that Plk1 phosphorylation disrupts this interaction revealed a transport-based mechanism for centrosome remodeling, while identification of Nek2 as a priming kinase established coordinate G2/M regulation.

    Evidence Reciprocal co-IP of NINL–dynactin, dominant-negative dynactin, in vitro kinase assays with Nek2 and Plk1, Xenopus localization studies

    PMID:15684383 PMID:16254247

    Open questions at the time
    • Precise phosphorylation sites mediating dynactin dissociation not mapped
    • In vivo kinase hierarchy not tested with endogenous kinase depletion
  3. 2010 Medium

    Identification of Cdc2/cyclin B1 phosphorylation at Ser185 as the Plk1-docking signal, and Aurora B phosphorylation directing NINL to the midbody, resolved how NINL transitions from centrosome to cytokinesis functions and established that deregulated NINL causes multinucleation.

    Evidence In vitro kinase assays, site-directed mutagenesis of Ser185/Ser448/Ser585, siRNA-mediated depletion with cytokinesis phenotyping

    PMID:20864540 PMID:20890132

    Open questions at the time
    • No in vivo confirmation of Aurora B–NINL interaction at physiological expression levels
    • Downstream midbody substrates or effectors of NINL unknown
  4. 2010 High

    Showing that NINL overexpression drives oncogenic transformation and spontaneous tumors in transgenic mice linked centrosome amplification to tumorigenesis and identified NINL as a candidate oncogene amplified in human lung cancer.

    Evidence Transgenic Nlp-overexpressing mice, soft agar, nude mouse xenograft, FISH on human lung cancer tissue

    PMID:20093778

    Open questions at the time
    • Mechanism connecting NINL overexpression to centrosome amplification not fully dissected
    • Whether NINL amplification is a driver or passenger in human cancer remains unresolved
  5. 2015 High

    Discovery that NINL interacts with CC2D2A and DZANK1, associates with complementary dynein-1 subunits, and is required for photoreceptor outer segment biogenesis and retrograde melanosome transport established NINL as a dynein activating adaptor for ciliary vesicle trafficking.

    Evidence Proteomic interactome, reciprocal co-IP, zebrafish morpholino knockdown/double knockdown with retinal and melanosome phenotypes, genetic epistasis

    PMID:26485514 PMID:26485645

    Open questions at the time
    • Whether NINL activates dynein processivity directly (as a canonical activating adaptor) was not biochemically reconstituted
    • Specific cargo molecules transported by the NINL–dynein complex in mammalian cilia not identified
  6. 2016 Medium

    Demonstrating UV-induced nuclear translocation of NINL and its interaction with XPA and ERCC1 to enhance NER activity revealed an unexpected DNA damage response function for a cytoplasmic/centrosomal protein.

    Evidence Nuclear translocation assay after UVC, co-IP with XPA/ERCC1, NER activity assay, domain deletion mapping

    PMID:26805762

    Open questions at the time
    • Nuclear import mechanism and NLS not defined
    • No independent replication of the NER role
    • Whether NER function is separable from centrosomal/trafficking roles unclear
  7. 2021 Medium

    Showing that NINL promotes autophagolysosome formation by enhancing Rab7–FYCO1 interaction and that Nlp-deficient mice have increased carcinogen-induced liver cancer due to autophagic defects expanded NINL's role to autophagy regulation.

    Evidence Co-IP of NINL–LC3/Rab7/FYCO1, autophagic flux assays, Nlp knockout mouse treated with DMBA

    PMID:33859171

    Open questions at the time
    • Whether NINL acts as a scaffold or allosteric activator for Rab7–FYCO1 not determined
    • Single lab finding; independent confirmation lacking
  8. 2022 High

    Demonstrating that NINL knockout cells have impaired interferon responses and that viral proteases from picornaviruses and coronaviruses cleave NINL established it as a key antiviral innate immune effector and a target of viral immune evasion.

    Evidence NINL knockout cell lines with viral replication assays, interferon response assays, in vitro protease cleavage, evolutionary selection analysis

    PMID:36222652

    Open questions at the time
    • Mechanism linking NINL/dynein adaptor function to interferon signaling not defined
    • Whether NINL cleavage is necessary and sufficient for immune evasion in vivo not shown
  9. 2024 Medium

    Identifying NINL as an ER-to-Golgi transport adaptor binding SEC31A and Rab1B, whose loss causes ER stress and UPR activation, revealed a COPII-linked trafficking function and connected NINL deficiency to spontaneous lymphomagenesis.

    Evidence Co-IP with SEC31A/Rab1B, cargo trafficking assays (β-Catenin, STING), Nlp knockout mice developing B cell lymphoma, ER stress markers

    PMID:38904019

    Open questions at the time
    • Whether NINL acts as a direct COPII adapter or an accessory factor not biochemically resolved
    • Lymphoma mechanism not dissected beyond ER stress association
    • Single lab; independent confirmation needed

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NINL coordinates its diverse functions—centrosome organization, ciliary trafficking, ER-to-Golgi transport, autophagy, NER, and innate immunity—across different cellular compartments remains unresolved, as does whether these reflect a unified dynein adaptor mechanism or independent moonlighting functions.
  • No structural model of NINL or its dynein-activating interface exists
  • Relative contributions of distinct NINL domains to each function not systematically mapped
  • Whether NINL loss causes a ciliopathy phenotype in humans is unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4 GO:0008092 cytoskeletal protein binding 3
Localization
GO:0005815 microtubule organizing center 5 GO:0031410 cytoplasmic vesicle 3 GO:0005794 Golgi apparatus 2 GO:0005829 cytosol 2 GO:0005634 nucleus 1
Pathway
R-HSA-1640170 Cell Cycle 4 R-HSA-1852241 Organelle biogenesis and maintenance 3 R-HSA-5653656 Vesicle-mediated transport 3 R-HSA-168256 Immune System 1 R-HSA-73894 DNA Repair 1 R-HSA-9612973 Autophagy 1
Partners
AURKBCC2D2ADCTN1DZANK1FYCO1NEK2PLK1SEC31A
Complex memberships
dynein-dynactin complex

Evidence

Reading pass · 13 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 NINL (Nlp, ninein-like protein) is a centrosomal substrate of Polo-like kinase 1 (Plk1). Plk1 phosphorylates Nlp, disrupts its centrosome association and its interaction with gamma-tubulin ring complex components, and thereby triggers Nlp displacement from the centrosome at mitotic onset. Nlp interacts with two components of the gamma-tubulin ring complex and stimulates microtubule nucleation during interphase. Co-immunoprecipitation, in vitro kinase assay, overexpression of phosphorylation-site mutants, immunofluorescence microscopy Developmental Cell High 12852856
2005 Nlp interacts with the dynein-dynactin motor complex, and this interaction is required for targeting Nlp (and ninein) to the centrosome. Phosphorylation of Nlp by Plk1 negatively regulates its association with dynactin, providing a mechanism by which Plk1 controls dynein-dynactin-dependent transport of centrosomal proteins. Overexpression of Nlp or ninein causes Golgi fragmentation and lysosome dispersal, dependent on their dynein-dynactin interaction. Co-immunoprecipitation, overexpression, dominant-negative dynactin, immunofluorescence, in vitro kinase assay Journal of Cell Science High 16254247
2005 Nlp is coordinately regulated at the G2/M transition by two centrosomal kinases, Nek2 and Plk1. Nek2 phosphorylates Nlp and can displace it from interphase centrosomes independently of Plk1 phosphorylation sites. Active Nek2 stimulates Plk1 phosphorylation of Nlp in vitro, suggesting Nek2 primes Nlp for Plk1 phosphorylation. Xenopus Nlp (X-Nlp) is a mother centriole-specific protein, implicating it in microtubule anchoring. In vitro kinase assay, overexpression of active/kinase-inactive Nek2 and Plk1, immunofluorescence, cell fractionation, co-immunoprecipitation Molecular and Cellular Biology High 15684383
2009 BRCA1 physically interacts and colocalizes with Nlp at centrosomes. BRCA1 regulates Nlp centrosomal localization and protein stability; cells with BRCA1 mutations or BRCA1 knockdown show disrupted Nlp centrosomal colocalization and enhanced Nlp degradation, likely via Plk1 de-repression. siRNA-mediated depletion of Nlp causes aberrant spindle formation, aborted chromosomal segregation, and aneuploidy. Co-immunoprecipitation, colocalization by immunofluorescence, BRCA1 siRNA/mutation, Nlp siRNA knockdown with phenotypic readout Journal of Biological Chemistry Medium 19509300
2010 Cdc2/cyclin B1 phosphorylates Nlp at Ser185 and Ser589. Phosphorylation at Ser185 is required for Plk1 recognition and subsequent Nlp displacement from centrosomes; Plk1 fails to dissociate an Nlp mutant lacking Ser185. Phosphorylation at Ser589 regulates Nlp protein stability/degradation. Deregulated Nlp expression or localization leads to multinucleation. In vitro kinase assay, site-directed mutagenesis, immunofluorescence, cell cycle synchronization Cancer Biology & Therapy Medium 20890132
2010 Aurora B physically interacts with Nlp and recruits it to the midbody during cytokinesis. Nlp is a substrate of Aurora B, phosphorylated at Ser-185, Ser-448, and Ser-585. Phosphorylation at Ser-448 and Ser-585 is required for Nlp association with Aurora B and midbody localization. Depletion of Nlp causes aborted cytokinesis and multinucleated phenotypes. Co-immunoprecipitation, in vitro kinase assay, site-directed mutagenesis, Nlp siRNA, immunofluorescence Journal of Biological Chemistry Medium 20864540
2010 Nlp overexpression confers oncogenic properties: NIH3T3 cells expressing Nlp gain anchorage-independent growth and form tumors in nude mice. Transgenic mice overexpressing Nlp develop spontaneous tumors in breast, ovary, and testis. Nlp overexpression causes centrosome amplification in mouse embryonic fibroblasts. NLP gene amplification was identified in human lung cancers. Transgenic mouse model, soft agar assay, xenograft, FISH gene amplification analysis, immunohistochemistry Journal of Clinical Investigation High 20093778
2015 NINL physically interacts with the ciliopathy protein CC2D2A and partially co-localizes at the base of cilia. Ninl knockdown in zebrafish causes photoreceptor outer segment loss, opsin mislocalization, and vesicle accumulation similar to cc2d2a mutant phenotypes. Partial ninl knockdown enhances the retinal phenotype of cc2d2a mutants (genetic interaction). NINL interactome analysis identifies MICAL3, a Rab8-interacting protein involved in vesicle docking/fusion, as an associated protein. Ninl morphants show altered Rab8a localization, supporting a role for NINL in cilia-directed vesicle trafficking. Co-immunoprecipitation, zebrafish knockdown (morpholino), genetic interaction analysis, co-localization by immunofluorescence, mass spectrometry interactome PLoS Genetics High 26485645
2015 NINL interacts with DZANK1 (Double Zinc Ribbon and Ankyrin Repeat domains 1), a novel binding partner. Loss of Ninl or Dzank1 in zebrafish causes dysmorphic photoreceptor outer segments, accumulation of trans-Golgi-derived vesicles, and mislocalization of Rhodopsin and Ush2a. Loss of both proteins synergistically worsens the phenotype. Retrograde melanosome transport is severely impaired. NINL and DZANK1 associate with complementary subunits of the cytoplasmic dynein 1 motor complex, suggesting they facilitate dynein complex assembly. Proteomic interaction screen, zebrafish knockdown/double knockdown, immunofluorescence, live imaging of melanosome transport, co-immunoprecipitation PLoS Genetics High 26485514
2016 Upon UVC irradiation, Nlp translocates from the cytoplasm/centrosome to the nucleus via its C-terminal domain (residues 1030-1382). In the nucleus, Nlp interacts with XPA and ERCC1, enhances their association, and improves nucleotide excision repair (NER) activity, protecting cells against UV radiation. Co-immunoprecipitation, immunofluorescence/nuclear translocation assay, NER activity assay, domain deletion mapping, siRNA knockdown Cancer Letters Medium 26805762
2021 Nlp colocalizes with autophagosomes during autophagy and physically interacts with LC3, Rab7, and FYCO1. Nlp enhances the interaction between Rab7 and FYCO1, accelerates autophagic flux, and promotes autophagolysosome formation. Nlp-deficient mice treated with DMBA show increased liver cancer incidence associated with hepatic autophagic defects. Co-immunoprecipitation, co-localization by immunofluorescence, autophagic flux assays, Nlp knockout mouse model, co-IP of Rab7-FYCO1 with/without Nlp Signal Transduction and Targeted Therapy Medium 33859171
2022 NINL functions as a dynein activating adaptor and is a critical component of the antiviral innate immune response. NINL knockout cells exhibit impaired interferon response and increased permissiveness to viral replication. Proteases encoded by diverse picornaviruses and coronaviruses cleave NINL and disrupt its function in a host- and virus-specific manner. NINL has evolved under recurrent positive selection, particularly in its carboxy-terminal cargo-binding region. NINL knockout cell lines, viral replication assays, interferon response assays, protease cleavage assays, evolutionary selection analysis eLife High 36222652
2024 Nlp acts as an adapter for ER-to-Golgi vesicle transport, directly binding SEC31A (a COPII coat component) and Rab1B to facilitate transport of specific cargo proteins including β-Catenin and STING. Nlp deficiency causes vesicle budding failure, ER accumulation of unprocessed proteins, ER stress, Golgi fragmentation, and activation of the PERK-eIF2α UPR pathway. Nlp-deficient mice develop spontaneous B cell lymphoma. Co-immunoprecipitation, Nlp knockout cells and mice, ER stress assays, cargo trafficking assays, immunofluorescence International Journal of Biological Sciences Medium 38904019

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2017 Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks. Nature 440 28489820
2015 An RLP23-SOBIR1-BAK1 complex mediates NLP-triggered immunity. Nature plants 351 27251392
2003 Polo-like kinase 1 regulates Nlp, a centrosome protein involved in microtubule nucleation. Developmental cell 215 12852856
2017 Interacting TCP and NLP transcription factors control plant responses to nitrate availability. Proceedings of the National Academy of Sciences of the United States of America 197 28202720
2021 The language of proteins: NLP, machine learning & protein sequences. Computational and structural biotechnology journal 193 33897979
2005 Phosphorylation of Nlp by Plk1 negatively regulates its dynein-dynactin-dependent targeting to the centrosome. Journal of cell science 86 16254247
2013 The nucleoplasmin homolog NLP mediates centromere clustering and anchoring to the nucleolus. Molecular cell 85 23562326
2005 Coordinate regulation of the mother centriole component nlp by nek2 and plk1 protein kinases. Molecular and cellular biology 80 15684383
2007 Impaired processing of FLP and NLP peptides in carboxypeptidase E (EGL-21)-deficient Caenorhabditis elegans as analyzed by mass spectrometry. Journal of neurochemistry 77 17564681
2012 The NLP toxin family in Phytophthora sojae includes rapidly evolving groups that lack necrosis-inducing activity. Molecular plant-microbe interactions : MPMI 75 22397404
2021 Different DNA-binding specificities of NLP and NIN transcription factors underlie nitrate-induced control of root nodulation. The Plant cell 73 33826745
2015 The Ciliopathy Protein CC2D2A Associates with NINL and Functions in RAB8-MICAL3-Regulated Vesicle Trafficking. PLoS genetics 70 26485645
2003 The crystal structure of Drosophila NLP-core provides insight into pentamer formation and histone binding. Structure (London, England : 1993) 68 12575937
2022 AttentionSiteDTI: an interpretable graph-based model for drug-target interaction prediction using NLP sentence-level relation classification. Briefings in bioinformatics 59 35817396
2002 Common and differential chemokine expression patterns in rs cells of NLP, EBV positive and negative classical Hodgkin lymphomas. International journal of cancer 57 12115499
2001 TARC, a CC chemokine, is frequently expressed in classic Hodgkin's lymphoma but not in NLP Hodgkin's lymphoma, T-cell-rich B-cell lymphoma, and most cases of anaplastic large cell lymphoma. The American journal of surgical pathology 53 11420464
2013 The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans. Nature communications 51 24301180
2014 Characterization of necrosis-inducing NLP proteins in Phytophthora capsici. BMC plant biology 47 24886309
2009 Life-span extension by dietary restriction is mediated by NLP-7 signaling and coelomocyte endocytosis in C. elegans. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 46 19783783
2007 Functional analysis of NLP genes from Botrytis elliptica. Molecular plant pathology 44 20507492
2020 Genome wide analysis of NLP transcription factors reveals their role in nitrogen stress tolerance of rice. Scientific reports 35 32523127
2016 Direct transcriptional activation of BT genes by NLP transcription factors is a key component of the nitrate response in Arabidopsis. Biochemical and biophysical research communications 34 28025145
2021 COVID-19 SignSym: a fast adaptation of a general clinical NLP tool to identify and normalize COVID-19 signs and symptoms to OMOP common data model. Journal of the American Medical Informatics Association : JAMIA 33 33674830
2010 Centrosomal Nlp is an oncogenic protein that is gene-amplified in human tumors and causes spontaneous tumorigenesis in transgenic mice. The Journal of clinical investigation 33 20093778
2003 Kinase pathway database: an integrated protein-kinase and NLP-based protein-interaction resource. Genome research 32 12799355
2002 Germline FAS gene mutation in a case of ALPS and NLP Hodgkin lymphoma. Blood 31 11830507
2018 Genome-Wide Identification and Characterization of NODULE-INCEPTION-Like Protein (NLP) Family Genes in Brassica napus. International journal of molecular sciences 30 30072649
2010 NLP is a novel transcription regulator involved in VSG expression site control in Trypanosoma brucei. Nucleic acids research 29 21076155
2018 Neuropeptides encoded by nlp-49 modulate locomotion, arousal and egg-laying behaviours in Caenorhabditis elegans via the receptor SEB-3. Philosophical transactions of the Royal Society of London. Series B, Biological sciences 28 30201834
2022 Identification of LINC00654-NINL Regulatory Axis in Diffuse Large B-Cell Lymphoma In Silico Analysis. Frontiers in oncology 26 35719990
2014 Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization. Genes & development 26 25228646
2015 NINL and DZANK1 Co-function in Vesicle Transport and Are Essential for Photoreceptor Development in Zebrafish. PLoS genetics 25 26485514
2009 BRCA1 interaction of centrosomal protein Nlp is required for successful mitotic progression. The Journal of biological chemistry 25 19509300
2018 Identification and functional analysis of the NLP-encoding genes from the phytopathogenic oomycete Phytophthora capsici. Molecular genetics and genomics : MGG 24 29572661
2017 Inappropriate Expression of an NLP Effector in Colletotrichum orbiculare Impairs Infection on Cucurbitaceae Cultivars via Plant Recognition of the C-Terminal Region. Molecular plant-microbe interactions : MPMI 24 29059009
2021 Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1. Signal transduction and targeted therapy 23 33859171
1989 NLP-1: a DNA intercalating hypoxic cell radiosensitizer and cytotoxin. International journal of radiation oncology, biology, physics 23 2703383
2022 An oomycete NLP cytolysin forms transient small pores in lipid membranes. Science advances 21 35275729
2021 An LRR-only protein promotes NLP-triggered cell death and disease susceptibility by facilitating oligomerization of NLP in Arabidopsis. The New phytologist 21 34403491
2019 Identification and Functional Analysis of NLP-Encoding Genes from the Postharvest Pathogen Penicillium expansum. Microorganisms 21 31208074
2018 Phylogenetic, expression and functional characterizations of the maize NLP transcription factor family reveal a role in nitrate assimilation and signaling. Physiologia plantarum 21 29364528
2011 Expression of an oxalate decarboxylase impairs the necrotic effect induced by Nep1-like protein (NLP) of Moniliophthora perniciosa in transgenic tobacco. Molecular plant-microbe interactions : MPMI 21 21405988
2015 NLP-12 engages different UNC-13 proteins to potentiate tonic and evoked release. The Journal of neuroscience : the official journal of the Society for Neuroscience 20 25609620
2011 The role of centrosomal Nlp in the control of mitotic progression and tumourigenesis. British journal of cancer 20 21505454
2008 Increased expression of Nlp, a potential oncogene in ovarian cancer, and its implication in carcinogenesis. Gynecologic oncology 20 18538832
2022 CollagenTransformer: End-to-End Transformer Model to Predict Thermal Stability of Collagen Triple Helices Using an NLP Approach. ACS biomaterials science & engineering 18 36149671
1989 Cloning and sequencing of an Escherichia coli gene, nlp, highly homologous to the ner genes of bacteriophages Mu and D108. Journal of bacteriology 17 2670911
2022 Antiviral function and viral antagonism of the rapidly evolving dynein activating adaptor NINL. eLife 15 36222652
2021 In silico analyses of neuropeptide-like protein (NLP) profiles in parasitic nematodes. International journal for parasitology 15 34450132
2009 Comparison of Caenorhabditis elegans NLP peptides with arthropod neuropeptides. Trends in parasitology 15 19269897
2021 Genome-wide survey and expression analysis of NIN-like Protein (NLP) genes reveals its potential roles in the response to nitrate signaling in tomato. BMC plant biology 14 34301191
2020 RPamide neuropeptides NLP-22 and NLP-2 act through GnRH-like receptors to promote sleep and wakefulness in C. elegans. Scientific reports 14 32555288
2012 Overexpression of centrosomal protein Nlp confers breast carcinoma resistance to paclitaxel. Cancer biology & therapy 14 22353935
2017 RNAi-mediated disruption of neuropeptide genes, nlp-3 and nlp-12, cause multiple behavioral defects in Meloidogyne incognita. Biochemical and biophysical research communications 13 28655614
2010 Aurora B interaction of centrosomal Nlp regulates cytokinesis. The Journal of biological chemistry 13 20864540
2010 Cdc2/cyclin B1 regulates centrosomal Nlp proteolysis and subcellular localization. Cancer biology & therapy 13 20890132
1999 Specific antibodies reactive with the 22-kilodalton major outer surface protein of Borrelia anserina Ni-NL protect chicks from infection. Infection and immunity 13 10225933
2024 Genome-wide identification and expression analysis of NIN-like protein (NLP) genes: Exploring their potential roles in nitrate response in tea plant (Camellia sinensis). Plant physiology and biochemistry : PPB 12 38199025
2023 Transcription factor module NLP-NIGT1 fine-tunes NITRATE TRANSPORTER2.1 expression. Plant physiology 12 37595050
1992 The Escherichia coli Mu/D108 phage ner homologue gene (nlp) is transcribed and evolutionarily conserved among the Enterobacteriaceae. Gene 12 1534062
2021 Automated NLP Extraction of Clinical Rationale for Treatment Discontinuation in Breast Cancer. JCO clinical cancer informatics 11 33989016
2012 Non Linear Programming (NLP) formulation for quantitative modeling of protein signal transduction pathways. PloS one 11 23226239
2020 Machine learning and natural language processing (NLP) approach to predict early progression to first-line treatment in real-world hormone receptor-positive (HR+)/HER2-negative advanced breast cancer patients. European journal of cancer (Oxford, England : 1990) 10 33373867
2015 Blending water- and nutrient-source wastewaters for cost-effective cultivation of high lipid content microalgal species Micractinium inermum NLP-F014. Bioresource technology 9 26409109
2008 Estimating relative risks for common outcome using PROC NLP. Computer methods and programs in biomedicine 9 18291556
2024 Using meta-analysis and CNN-NLP to review and classify the medical literature for normal tissue complication probability in head and neck cancer. Radiation oncology (London, England) 8 38195582
2022 Stemphylium lycopersici Nep1-like Protein (NLP) Is a Key Virulence Factor in Tomato Gray Leaf Spot Disease. Journal of fungi (Basel, Switzerland) 8 35628773
2022 NLP-BCH-Ens: NLP-based intelligent computational model for discrimination of malaria parasite. Computers in biology and medicine 8 36049412
2024 An NLP-based technique to extract meaningful features from drug SMILES. iScience 7 38455979
2022 Triplication is the main evolutionary driving force of NLP transcription factor family in Chinese cabbage and related species. International journal of biological macromolecules 7 35051503
2006 Developing an NLP and IR-based algorithm for analyzing gene-disease relationships. Methods of information in medicine 7 16685344
2024 Caenorhabditis elegans neuropeptide NLP-27 enhances neurodegeneration and paralysis in an opioid-like manner during fungal infection. iScience 6 38784855
2021 The CCCH-Type Zinc Finger Antiviral Protein Relieves Immunosuppression of T Cells Induced by Avian Leukosis Virus Subgroup J via the NLP-PKC-δ-NFAT Pathway. Journal of virology 6 34705559
2020 COVID-19 SignSym: a fast adaptation of a general clinical NLP tool to identify and normalize COVID-19 signs and symptoms to OMOP common data model. ArXiv 6 32908948
2018 Mining protein phosphorylation information from biomedical literature using NLP parsing and Support Vector Machines. Computer methods and programs in biomedicine 6 29728247
2015 Synthesis and characterization of Her2-NLP peptide conjugates targeting circulating breast cancer cells: cellular uptake and localization by fluorescent microscopic imaging. Journal of fluorescence 6 25620472
2015 Mass Spectrometry of Single GABAergic Somatic Motorneurons Identifies a Novel Inhibitory Peptide, As-NLP-22, in the Nematode Ascaris suum. Journal of the American Society for Mass Spectrometry 6 26174364
2025 Nitrate activates an MKK3-dependent MAPK module via NLP transcription factors in Arabidopsis. The Plant journal : for cell and molecular biology 5 39962336
2020 Triage of documents containing protein interactions affected by mutations using an NLP based machine learning approach. BMC genomics 5 33167858
2023 Identification and expression characteristics of NLP (NIN-like protein) gene family in pepper (Capsicum annuum L.). Molecular biology reports 4 37358766
2022 Efficient E-Mail Spam Detection Strategy Using Genetic Decision Tree Processing with NLP Features. Computational intelligence and neuroscience 4 35371228
2021 Tuning Selective Transport of Biomolecules through Site-Mutated Nucleoporin-like Protein (NLP) Hydrogels. Biomacromolecules 4 33428378
2016 Mitotic regulator Nlp interacts with XPA/ERCC1 complexes and regulates nucleotide excision repair (NER) in response to UV radiation. Cancer letters 4 26805762
2011 A pharmacological study of NLP-12 neuropeptide signaling in free-living and parasitic nematodes. Peptides 4 22019590
2024 Nlp-dependent ER-to-Golgi transport. International journal of biological sciences 3 38904019
2022 Genome-Wide Analysis and Evolutionary History of the Necrosis- and Ethylene-Inducing Peptide 1-Like Protein (NLP) Superfamily Across the Dothideomycetes Class of Fungi. Current microbiology 3 36536087
2002 DNA-targeted 2-nitroimidazoles: studies of the influence of the phenanthridine-linked nitroimidazoles, 2-NLP-3 and 2-NLP-4, on DNA damage induced by ionizing radiation. Radiation research 3 12175307
2025 Hard label adversarial attack with high query efficiency against NLP models. Scientific reports 2 40102502
2025 Interdependence between SEB-3 receptor and NLP-49 peptides shifts across predator-induced defensive behavioral modes in Caenorhabditis elegans. eLife 2 40163376
2024 GeNLP: a web tool for NLP-based exploration and prediction of microbial gene function. Bioinformatics (Oxford, England) 2 38291951
2024 Temperature-dependent lifespan extension is achieved in miR-80-deleted Caenorhabditis elegans by NLP-45 to modulate endoplasmic reticulum unfolded protein responses. Aging cell 2 39323014
2024 The Potential of Using Generative AI/NLP to Identify and Analyse Critical Incidents in a Critical Incident Reporting System (CIRS): A Feasibility Case-Control Study. Healthcare (Basel, Switzerland) 2 39408144
2023 Bioactive Peptide Recognition Based on NLP Pre-Train Algorithm. IEEE/ACM transactions on computational biology and bioinformatics 2 37815965
2022 The necrosis- and ethylene-inducing peptide 1-like protein (NLP) gene family of the plant pathogen Corynespora cassiicola. Current genetics 2 36098767
2024 Identification, molecular evolution, codon bias, and expansion analysis of NLP transcription factor family in foxtail millet (Setaria italica L.) and closely related crops. Frontiers in genetics 1 38836039
2024 Genome-Wide Identification of NLP Gene Families and Haplotype Analysis of SiNLP2 in Foxtail Millet (Setaria italica). International journal of molecular sciences 1 39684649
2024 Circular RNA NINL accelerates the malignant progression of cervical cancer. Discover oncology 1 39692846
2023 m-NLP Inference Models Using Simulation and Regression Techniques. Journal of geophysical research. Space physics 1 37035843
2023 A large-scale evaluation of NLP-derived chemical-gene/protein relationships from the scientific literature: Implications for knowledge graph construction. PloS one 1 37682956
2022 RegenX: an NLP recommendation engine for neuroregeneration topics over time. Annals of eye science 1 36199680