Affinage

NEXN

Nexilin · UniProt Q0ZGT2

Length
675 aa
Mass
80.7 kDa
Annotated
2026-06-10
43 papers in source corpus 22 papers cited in narrative 22 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

Nexilin (NEXN) is an F-actin-binding cytoskeletal protein that organizes contractile and adhesive actin structures and serves as an essential structural component of the cardiac sarcomere and dyad (PMID:9832551, PMID:19881492, PMID:30982350). It contains multiple actin-binding domains, cross-links F-actin, and localizes to cell-matrix adherens junctions in non-muscle cells and to the Z-disk—and the smooth-muscle equivalent dense bodies—in striated and smooth muscle (PMID:9832551, PMID:19881492, PMID:30158653). At the Z-disk, nexilin protects against mechanical strain, and its loss destabilizes Z-disk ultrastructure and causes heart failure, with disease-associated mutant alleles acting dominant-negatively and abolishing actin binding (PMID:19881492, PMID:20970104). Nexilin is a component of junctional membrane complexes at cardiac dyads, where it interacts with junctional sarcoplasmic reticulum proteins and is required for both developmental formation and adult maintenance of the transverse-tubular system, optimal calcium transients, and excitation-contraction coupling; its loss in mice and human iPSC-derived cardiomyocytes produces dilated cardiomyopathy with disordered dyads, impaired calcium handling, and metabolic stress (PMID:30982350, PMID:32635769, PMID:40713745). Loss-of-function cardiomyopathy is rescuable by AAV-delivered full-length nexilin in knockout and human-equivalent G645del mutant mice, establishing NEXN as a causative dilated/hypertrophic cardiomyopathy gene (PMID:38783323, PMID:19881492). NEXN activity is post-translationally tuned by CLK4, which directly phosphorylates it to restrain pathological cardiomyocyte hypertrophy (PMID:35907876). Beyond the heart, nexilin governs vascular smooth muscle phenotype—promoting the contractile state via RhoA/SRF and protecting against calcification through a SERCA2 interaction that enhances SERCA2 SUMOylation and stability (PMID:26458985, PMID:40883305). Additional context-specific roles include scaffolding insulin/IRS1 signaling in skeletal myotubes (PMID:23383252), inhibiting TLR4 oligomerization and NF-κB-driven endothelial inflammation (PMID:30589415), and, in non-muscle cells, mediating actin-dependent organelle dynamics including late endosome/lysosome fission via Rab7b and TRPML1 and surface trafficking of γ2-containing GABAA receptors in neurons (PMID:40812511, PMID:41514273).

Mechanistic history

Synthesis pass · year-by-year structured walk · 17 steps
  1. 1998 High

    Established the founding biochemical identity of nexilin as an F-actin-binding and cross-linking protein with distinct domain architectures and junctional localization, defining the molecular basis for all later cytoskeletal roles.

    Evidence Protein purification with in vitro F-actin binding/cross-linking assays and immunofluorescence in rat brain and fibroblasts

    PMID:9832551

    Open questions at the time
    • No tissue-level or in vivo function established
    • Physiological consequences of cross-linking activity unaddressed
  2. 2005 Medium

    First linked nexilin to cell behavior, showing its overexpression promotes migration and adhesion, extending its actin association to functional motility.

    Evidence Co-IP and stable overexpression with migration/adhesion assays in HeLa cells

    PMID:15823560

    Open questions at the time
    • Overexpression-only; no loss-of-function
    • Mechanism connecting actin binding to migration not defined
  3. 2009 High

    Identified nexilin as a Z-disk protein essential for sarcomere stability and protection against mechanical strain, and connected human NEXN mutations to cardiomyopathy via dominant-negative Z-disk damage.

    Evidence Zebrafish morpholino knockdown, mutant allele expression, Z-disk electron microscopy, human genetic association

    PMID:19881492

    Open questions at the time
    • Molecular partners at the Z-disk not defined
    • Mechanism of strain protection unresolved
  4. 2010 Medium

    Showed disease-associated NEXN missense mutations mechanistically impair F-actin/α-actin binding and cause protein mislocalization, tying genotype to a defined biochemical defect.

    Evidence Transfection, immunofluorescence, Co-IP, F-actin binding assay in C2C12 cells

    PMID:20970104

    Open questions at the time
    • Single-lab in vitro correlation
    • In vivo consequence of each mutation not tested
  5. 2013 Medium

    Revealed a metabolic scaffolding role in which nexilin binds IRS1 in an actin-dependent, insulin-regulated manner to restrain PI3K/AKT signaling and glucose uptake in muscle.

    Evidence Reciprocal Co-IP, siRNA/overexpression, PI3K and AKT readouts, glucose uptake in L6 myotubes

    PMID:23383252

    Open questions at the time
    • Single-lab cell model only
    • In vivo metabolic relevance untested
  6. 2014 Medium

    Identified a developmental signaling role whereby NEXN negatively regulates GATA4 and cardiac differentiation, with disease mutations recapitulating GATA4 suppression.

    Evidence Knockdown/overexpression/rescue in P19cl6 cells and cardiac-selective transgenic mice

    PMID:24866383

    Open questions at the time
    • Mechanism of GATA4 suppression unknown
    • Relationship to structural Z-disk role unclear
  7. 2015 Medium

    Defined nexilin as a driver of smooth muscle phenotype, promoting the contractile state through RhoA-dependent SRF nuclear translocation.

    Evidence Lentiviral OE/siRNA in VSMCs with RhoA/SRF/SMα-actin blots and Y-27632 inhibitor

    PMID:26458985

    Open questions at the time
    • Direct RhoA regulatory mechanism not defined
    • Single-lab pharmacological inference
  8. 2015 High

    Demonstrated an essential in vivo cardiac requirement, with constitutive Nexn knockout producing early, rapidly progressive dilated cardiomyopathy.

    Evidence Constitutive KO mouse, echocardiography, histology, immunostaining

    PMID:26659360

    Open questions at the time
    • Cell-autonomous vs developmental contribution not separated
    • Molecular cause of DCM not yet mechanistic
  9. 2018 Medium

    Placed NEXN within a transcriptional feedback circuit in smooth muscle, controlled by actin state and MRTF/YAP-TAZ coactivators and feeding back on actin polymerization.

    Evidence Immunoelectron microscopy, Latrunculin B, KD/OE, MRTF/YAP overexpression, promoter assay

    PMID:30158653

    Open questions at the time
    • Direct promoter occupancy not shown
    • In vivo SMC relevance untested
  10. 2019 High

    Resolved the dilated-cardiomyopathy mechanism by identifying NEXN as a junctional membrane complex component required for T-tubule formation, calcium transients, and excitation-contraction coupling.

    Evidence Global and cardiomyocyte-specific KO mice with EM, calcium imaging, RNA-seq, and interaction mass spectrometry

    PMID:30982350

    Open questions at the time
    • Specific jSR-binding partners not individually validated
    • How nexilin nucleates T-tubule invagination mechanistically unresolved
  11. 2019 Medium

    Uncovered a vascular protective, anti-inflammatory function in which NEXN inhibits TLR4 oligomerization and NF-κB signaling downstream of lncRNA NEXN-AS1.

    Evidence KD/OE, TLR4 oligomerization assay, NF-κB reporter, monocyte adhesion, ApoE-KO mouse model

    PMID:30589415

    Open questions at the time
    • Direct TLR4 binding not structurally defined
    • Link to cytoskeletal function unclear
  12. 2020 High

    Showed NEXN is required not only for developmental T-tubule formation but for ongoing maintenance of tubular architecture in adult cardiomyocytes.

    Evidence Inducible adult cardiomyocyte-specific KO with quantitative T-tubule morphometry, calcium imaging, echocardiography

    PMID:32635769

    Open questions at the time
    • Turnover mechanism of tubular maintenance unresolved
    • Reversibility upon re-expression not tested here
  13. 2022 High

    Identified CLK4 as the kinase that directly phosphorylates nexilin to restrain pathological hypertrophy, defining a post-translational control layer for NEXN.

    Evidence Kinase substrate assay, cardiomyocyte-specific Clk4 KO mice, phospho-mimic NEXN rescue, cardiomyocyte size measurements

    PMID:35907876

    Open questions at the time
    • Phosphosite-specific structural consequence not mapped
    • Whether phosphorylation alters actin or dyad function unknown
  14. 2024 Medium

    Demonstrated therapeutic sufficiency, with AAV-delivered full-length nexilin rescuing cardiac function and lifespan in KO and G645del mutant mice and defining minimal functional components.

    Evidence Systemic AAV gene delivery, echocardiography, immunoblot in two mouse models

    PMID:38783323

    Open questions at the time
    • Durability and dosing not fully characterized
    • Single-lab rescue
  15. 2025 Medium

    Confirmed in a human system that NEXN is required for junctional membrane complex integrity and excitation-contraction coupling, linking its loss to oxidative and metabolic stress.

    Evidence CRISPR KO in human iPSC-derived cardiomyocytes with EM, calcium imaging, oxidative stress and metabolic assays

    PMID:40713745

    Open questions at the time
    • Single-lab hiPSC model
    • Causal order of metabolic vs structural defects unresolved
  16. 2025 High

    Expanded NEXN's vascular role by showing it stabilizes SERCA2 via enhanced SUMOylation to prevent calcification, and that its deficiency drives VSMC phenotypic transition through ER stress/KLF4 signaling.

    Evidence VSMC-specific KO/OE mice, Co-IP, SUMOylation assay, lineage tracing, ER stress inhibitor rescue

    PMID:40440261 PMID:40883305

    Open questions at the time
    • Direct SERCA2 binding interface not mapped
    • How NEXN promotes SUMOylation mechanistically unresolved
  17. 2025 Medium

    Extended nexilin function to neuronal and organelle biology, mapping direct binding to GABAA receptor γ-subunits and to Rab7b/TRPML1 for actin-dependent surface receptor trafficking and late endosome/lysosome fission.

    Evidence Peptide array mapping, deep-mutational scanning, ABD-deletion mutants, electrophysiology, Co-IP, TRPML1 agonist rescue in neurons and cells

    PMID:40812511 PMID:41514273

    Open questions at the time
    • In vivo neuronal relevance untested
    • How actin binding couples to organelle fission not fully defined

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how nexilin's single actin-binding scaffold is repurposed across such divergent contexts (sarcomere/dyad, vascular SERCA2 stabilization, IRS1/TLR4 signaling, organelle fission) and whether tissue-specific isoforms partition these functions.
  • Isoform-specific functions only mapped at recombinant/expression level [#21]
  • No unifying structural mechanism linking actin binding to signaling roles

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 4 GO:0098772 molecular function regulator activity 3 GO:0060090 molecular adaptor activity 2
Localization
GO:0005856 cytoskeleton 3 GO:0005764 lysosome 2 GO:0005783 endoplasmic reticulum 2 GO:0005886 plasma membrane 2
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-397014 Muscle contraction 3 R-HSA-1266738 Developmental Biology 2 R-HSA-5653656 Vesicle-mediated transport 1
Partners
CLK4GABRG2IRS1MYOCDRAB7BSERCA2TLR4TRPML1
Complex memberships
Z-diskcardiac junctional membrane complex (dyad)smooth muscle dense bodies

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 Nexilin (b-nexilin and s-nexilin) are F-actin binding proteins isolated from rat brain and fibroblasts. b-Nexilin has two F-actin-binding domains (ABDs) at NH2-terminal and middle regions and shows F-actin cross-linking activity; s-nexilin has one ABD at the middle region and lacks cross-linking activity. s-Nexilin colocalizes with vinculin, talin, and paxillin at cell-matrix adherens junctions and focal contacts. Protein purification, in vitro F-actin binding assay, F-actin cross-linking assay, immunofluorescence microscopy The Journal of cell biology High 9832551
2009 Nexilin is a Z-disk protein in cardiac and skeletal muscle; loss of nexilin in zebrafish disrupts Z-disk stability and causes heart failure. Expression of disease-associated NEXN mutant alleles in zebrafish induces Z-disk damage in a dominant-negative manner. Increased mechanical strain aggravates Z-disk damage in nexilin-deficient skeletal muscle, indicating nexilin protects Z-disks from mechanical trauma. Zebrafish morpholino knockdown, transgenic expression of mutant NEXN alleles, electron microscopy of Z-disk ultrastructure, genetic association study Nature medicine High 19881492
2005 NELIN (human nexilin) product associates with F-actin in cells. Stable transfection of NELIN into HeLa cells increases cell migration by ~2-fold and adhesion by ~1.7-fold compared to empty vector controls. Immunofluorescence, immunoprecipitation, stable transfection, migration and adhesion assays Biochemical and biophysical research communications Medium 15823560
2010 Two HCM-associated NEXN missense mutations (Q131E and R279C) cause local accumulations of nexilin in cells. The Q131E mutation abolishes F-actin binding of the actin-binding domain fragment and decreases binding of full-length NEXN to α-actin as demonstrated by co-immunoprecipitation. Cellular transfection, immunofluorescence, co-immunoprecipitation, F-actin binding assay in C2C12 cells American journal of human genetics Medium 20970104
2013 Nexilin binds preferentially to IRS1 (over IRS2) in L6 skeletal muscle myotubes under basal conditions, and this complex is disassembled by insulin. Functional silencing of nexilin enhances recruitment of p85α to IRS1, increases PI-3,4,5-P3 formation, and enhances AKT activation and glucose uptake, while nexilin overexpression inhibits IRS1-to-AKT signaling. Disruption of actin with Latrunculin B abolishes the nexilin–IRS1 spatial association. Co-immunoprecipitation, siRNA knockdown, nexilin overexpression, PI3K activity assay, AKT phosphorylation immunoblot, glucose uptake assay in L6 myotubes PloS one Medium 23383252
2014 NEXN inhibits cardiac differentiation by suppressing GATA4 expression. Cardiac-selective NEXN transgenic mice develop atrial septal defects. Disease-associated NEXN mutations (K199E, L227S) also inhibit GATA4 expression, establishing NEXN as a negative regulator of GATA4-dependent cardiac development. Knockdown, overexpression, and rescue experiments in P19cl6 cells; transgenic mouse model; GATA4 expression analysis Cardiovascular research Medium 24866383
2015 Constitutive Nexn knockout mice develop rapidly progressive dilated cardiomyopathy with left ventricular dilation, wall thinning, systolic dysfunction, and collagen/elastin deposits (endomyocardial fibroelastosis) by postnatal day 6, establishing an essential role of nexilin in cardiac structure and function. Constitutive knockout mouse model, echocardiography, histology, immunostaining Basic research in cardiology High 26659360
2018 Nexilin localizes to dense bodies and dense bands in smooth muscle cells (analogous to Z-discs). NEXN expression in SMCs is controlled by actin polymerization state and by transcriptional coactivators MRTF (MYOCD, MKL1, MKL2) and YAP/TAZ. Silencing NEXN reduces actin polymerization, cell migration, and smooth muscle marker expression, indicating a positive feedback loop between nexilin and actin dynamics. Immunofluorescence, immunoelectron microscopy, Latrunculin B treatment, NEXN knockdown/overexpression, MRTF/YAP overexpression, promoter assay Scientific reports Medium 30158653
2019 NEXN is a component of junctional membrane complexes at cardiac dyads. Loss of Nexn in global and cardiomyocyte-specific knockout mice causes dilated cardiomyopathy. NEXN interacts with junctional sarcoplasmic reticulum proteins, is essential for optimal calcium transients, and is required for initiation and formation of T-tubule invagination. Global and cardiomyocyte-specific KO mice, electron microscopy, confocal microscopy, calcium transient measurements, RNA sequencing, mass spectrometry Circulation High 30982350
2019 NEXN (nexilin) exerts a protective role against atherosclerosis; NEXN protein inhibits TLR4 oligomerization and downstream NF-κB activity, reducing expression of adhesion molecules and inflammatory cytokines in endothelial cells. The anti-inflammatory/anti-atherosclerotic effects of the lncRNA NEXN-AS1 are abolished by NEXN knockdown, placing NEXN downstream of NEXN-AS1 in this pathway. In ApoE-KO mice, NEXN deficiency promotes atherosclerosis while augmented NEXN expression deters it. In vitro knockdown/overexpression, TLR4 oligomerization assay, NF-κB reporter, monocyte adhesion assay, ApoE-KO mouse model with NEXN manipulation The Journal of clinical investigation Medium 30589415
2020 Inducible adult cardiomyocyte-specific loss of Nexn in mice causes dilated cardiomyopathy, impairs calcium handling, and leads to a 40% reduction in the transverse tubular component, demonstrating that NEXN is required not only for T-tubule formation during development but also for maintenance of the transverse-axial tubular architecture in adult cardiomyocytes. Inducible cardiomyocyte-specific KO mice, confocal microscopy, electron microscopy, calcium transient and myocyte shortening studies, echocardiography Circulation. Heart failure High 32635769
2022 CDC-like kinase 4 (CLK4) directly phosphorylates nexilin (NEXN). CLK4 knockdown induces pathological cardiomyocyte hypertrophy, while overexpression of a phosphorylation-mimic NEXN mutant is sufficient to reverse CLK4 knockdown-induced hypertrophy. Restoring NEXN phosphorylation ameliorates myocardial hypertrophy in cardiac-specific Clk4-knockout mice, establishing CLK4 as the kinase writer for NEXN phosphorylation in cardiac hypertrophy regulation. Kinase substrate assay, cardiomyocyte-specific Clk4 KO mice, phosphorylation-mimic NEXN mutant overexpression, echocardiography, cardiomyocyte size measurements Nature communications High 35907876
2015 NELIN (nexilin) promotes vascular smooth muscle cells (VSMCs) toward a contractile phenotype by activating RhoA, which drives SRF nuclear translocation and SMα-actin expression. NELIN knockdown shifts VSMCs to a synthetic phenotype with decreased RhoA and SRF nuclear localization; the RhoA kinase inhibitor Y-27632 abolishes the NELIN-induced contractile phenotype. Lentiviral overexpression and siRNA knockdown in VSMCs, Western blot for RhoA/SRF/SMα-actin, nuclear translocation assay, Rho kinase inhibitor treatment Molecular medicine reports Medium 26458985
2025 NEXN interacts with SERCA2 in vascular smooth muscle cells, enhancing SERCA2 SUMOylation, stability, and function. VSMC-specific NEXN knockout exacerbates vascular calcification, while NEXN overexpression alleviates it, operating through the NEXN-SERCA2 interaction and SERCA2 SUMOylation axis. VSMC-specific KO and overexpression mouse models, co-immunoprecipitation for NEXN-SERCA2 interaction, SUMOylation assay, multi-transcriptomics analysis Nature communications High 40883305
2025 NEXN deficiency in VSMCs promotes phenotypic transition and neointimal hyperplasia through endoplasmic reticulum (ER) stress and Krüppel-like factor 4 (KLF4) signaling. Inhibiting ER stress ameliorates VSMC phenotypic transition and proliferation caused by NEXN deficiency. VSMC-specific lineage-tracing mice, NEXN KO, integrative transcriptomics, ER stress inhibitor treatment, cell proliferation and phenotypic marker assays JCI insight Medium 40440261
2025 Nexilin binds directly to the C-terminal intracellular loop of GABAA receptor γ-subunits. This interaction regulates cell-surface expression of extrasynaptic γ2-containing GABAA receptors in hippocampal neurons; nexilin upregulation increases surface GABAA receptor numbers and synaptic currents, while the effect requires the actin-binding domain of nexilin (its deletion abolishes the effect). Nexilin also interacts with Rab7b and the lysosomal calcium channel TRPML1, and promotes calcium-dependent fission of late endosomes/lysosomes required for retrograde transport. Pulldown, array-based peptide mapping, deep-mutational scanning, siRNA knockdown and overexpression in hippocampal neurons, electrophysiology (GABA-mediated currents), surface GABAA receptor quantification, actin-binding domain deletion mutant Neuropharmacology Medium 40812511
2026 Nexilin regulates late endosome/lysosome (LE/Lys) fission and retrograde transport. Depletion of nexilin causes LE/Lys enlargement due to inhibited fission. Nexilin interacts with the small GTPase Rab7b and the lysosomal calcium channel TRPML1; TRPML1 activation rescues LE/Lys enlargement caused by nexilin depletion, indicating nexilin mediates the interaction between LE/Lys and the acto-myosin cytoskeleton for calcium-dependent organelle fission. siRNA screen, live imaging of LE/Lys size, Co-immunoprecipitation for Rab7b and TRPML1, TRPML1 agonist rescue experiment, retrograde transport assay Cell communication and signaling : CCS Medium 41514273
2012 Nexilin is required for efficient Listeria monocytogenes invasion; siRNA-mediated nexilin knockdown significantly reduces intracellular bacterial levels. Nexilin is a component of L. monocytogenes actin comet tails and EPEC pedestals, accumulating at the distal portion of motile bacterial actin structures. Nexilin knockdown impairs comet tail formation (malformed comet tails) but is dispensable for EPEC pedestal generation. siRNA knockdown, immunofluorescence colocalization, bacterial invasion assay (intracellular bacteria quantification), comet tail morphology analysis Cellular microbiology Medium 22381134
2025 NEXN overexpression in hepatocellular carcinoma cells reduces β-catenin nuclear accumulation and inhibits EMT. NEXN binds to MYOCD, and together they co-regulate EMT via the WNT/β-catenin signaling pathway. Diminished NEXN expression leads to β-catenin nuclear accumulation and enhanced EMT-driven metastasis. Overexpression in HCC cell lines, β-catenin nuclear localization assay, Co-immunoprecipitation for NEXN-MYOCD interaction, in vivo tumor formation, EMT marker Western blot iScience Low 41399508
2024 AAV-mediated systemic delivery of Nexn restores cardiac function and extends lifespan in both global Nexn knockout mice and mice carrying the human equivalent G645del mutation, demonstrating that exogenous full-length nexilin can functionally rescue loss-of-function DCM and identifying functional components of nexilin sufficient for this rescue. AAV gene delivery (systemic intravenous injection), echocardiography, immunoblot, Nexn global KO and G645del knock-in mouse models Genome biology Medium 38783323
2025 NEXN homozygous knockout human iPSC-derived cardiomyocytes show disordered junctional membrane complexes, abnormal excitation-contraction coupling, increased oxidative stress, and decreased energy metabolism, confirming that nexilin is a structural component of junctional membrane complexes essential for excitation-contraction coupling. CRISPR/Cas9 knockout in hiPSCs, directed differentiation to cardiomyocytes, electron microscopy, calcium imaging, oxidative stress assays, metabolic assays Stem cell research & therapy Medium 40713745
2024 Novel nexilin splice variants exist in mouse and human tissues segregating into myocyte-specific and epithelial-specific isoforms. Heart-specific isoforms differ between atria and ventricles. Different isoforms exhibit distinct self-interaction properties in recombinant protein interaction studies, and critical exons in ABD1 and ABD2 differ between isoforms. RT-PCR, tissue expression analysis, recombinant protein interaction studies (pulldown), isoform mapping Cells Low 39682766

Source papers

Stage 0 corpus · 43 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2009 Nexilin mutations destabilize cardiac Z-disks and lead to dilated cardiomyopathy. Nature medicine 172 19881492
2019 Long noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN. The Journal of clinical investigation 119 30589415
2010 Mutations in NEXN, a Z-disc gene, are associated with hypertrophic cardiomyopathy. American journal of human genetics 85 20970104
2019 Atorvastatin inhibits pyroptosis through the lncRNA NEXN-AS1/NEXN pathway in human vascular endothelial cells. Atherosclerosis 80 31830726
2019 Nexilin Is a New Component of Junctional Membrane Complexes Required for Cardiac T-Tubule Formation. Circulation 72 30982350
1998 Nexilin: a novel actin filament-binding protein localized at cell-matrix adherens junction. The Journal of cell biology 66 9832551
2016 A Novel Genetic Variant in Long Non-coding RNA Gene NEXN-AS1 is Associated with Risk of Lung Cancer. Scientific reports 39 27713484
2019 Whole genome and transcriptome sequencing of post-mortem cardiac tissues from sudden cardiac death victims identifies a gene regulatory variant in NEXN. International journal of legal medicine 37 31392414
2015 Knock-out of nexilin in mice leads to dilated cardiomyopathy and endomyocardial fibroelastosis. Basic research in cardiology 35 26659360
2005 NELIN, a new F-actin associated protein, stimulates HeLa cell migration and adhesion. Biochemical and biophysical research communications 32 15823560
2020 Nexilin Is Necessary for Maintaining the Transverse-Axial Tubular System in Adult Cardiomyocytes. Circulation. Heart failure 30 32635769
2018 Nexilin/NEXN controls actin polymerization in smooth muscle and is regulated by myocardin family coactivators and YAP. Scientific reports 29 30158653
2014 NEXN inhibits GATA4 and leads to atrial septal defects in mice and humans. Cardiovascular research 26 24866383
2013 Nexilin, a cardiomyopathy-associated F-actin binding protein, binds and regulates IRS1 signaling in skeletal muscle cells. PloS one 23 23383252
2021 Childhood onset nexilin dilated cardiomyopathy: A heterozygous and a homozygous case. American journal of medical genetics. Part A 21 33949776
2018 Nexilin Regulates Oligodendrocyte Progenitor Cell Migration and Remyelination and Is Negatively Regulated by Protease-Activated Receptor 1/Ras-Proximate-1 Signaling Following Subarachnoid Hemorrhage. Frontiers in neurology 21 29922213
2013 NEXN is a novel susceptibility gene for coronary artery disease in Han Chinese. PloS one 20 24349201
2022 Loss of Nexilin function leads to a recessive lethal fetal cardiomyopathy characterized by cardiomegaly and endocardial fibroelastosis. American journal of medical genetics. Part A 18 35166435
2022 CDC-like kinase 4 deficiency contributes to pathological cardiac hypertrophy by modulating NEXN phosphorylation. Nature communications 15 35907876
2015 Regulatory mechanism of human vascular smooth muscle cell phenotypic transformation induced by NELIN. Molecular medicine reports 9 26458985
2023 CRISPR/Cas9-mediated nexilin deficiency interferes with cardiac contractile function in zebrafish in vivo. Scientific reports 8 38114601
2001 Molecular Cloning of NELIN, a Putative Human Cytoskeleton Regulation Gene. Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica 8 12053183
2012 Nexilin is a dynamic component of Listeria monocytogenes and enteropathogenic Escherichia coli actin-rich structures. Cellular microbiology 7 22381134
2025 NEXN protects against vascular calcification by promoting SERCA2 SUMOylation and stabilization. Nature communications 6 40883305
2025 Nexilin mutations, a cause of chronic heart failure: A state-of-the-art review starting from a clinical case. World journal of cardiology 5 40161564
2024 In vivo rescue of genetic dilated cardiomyopathy by systemic delivery of nexilin. Genome biology 5 38783323
2024 Biallelic NEXN variants and fetal onset dilated cardiomyopathy: two independent case reports and revision of literature. Italian journal of pediatrics 5 39183344
2025 Anti-atherosclerotic effects of LncRNA NEXN-AS1 by regulation of canonical inflammasome pathway of pyroptosis via NEXN. Folia histochemica et cytobiologica 4 39801217
2025 Genetic and Phenotypic Characterization of Nexilin (NEXN)-Related Cardiomyopathy: Results From a Multicentric Study. JACC. Heart failure 4 40680702
2015 Expression and significance of NELIN and SM22α in varicose vein tissue. Experimental and therapeutic medicine 4 25667639
2025 NEXN regulates vascular smooth muscle cell phenotypic switching and neointimal hyperplasia. JCI insight 3 40440261
2022 Two genetic variants in NEXN and ABCC6 genes found in a patient with right coronary artery to right ventricle fistula combined with giant coronary aneurysm and patent ductus arteriosus. Frontiers in cardiovascular medicine 3 36465446
2025 Identifying nexilin as a central gene in neutrophil-driven abdominal aortic aneurysm pathogenesis. Molecular medicine (Cambridge, Mass.) 2 40140755
2025 NEXN deficiency leads to dilated cardiomyopathy in human pluripotent stem cell-derived cardiomyocytes. Stem cell research & therapy 2 40713745
2024 Nexilin in cardiomyopathy: unveiling its diverse roles with special focus on endocardial fibroelastosis. Heart failure reviews 2 38985384
2019 LncRNA NEXN-AS1 attenuates proliferation and migration of vascular smooth muscle cells through sponging miR-33a/b. RSC advances 2 35530470
2025 LncRNA NEXN-AS1 Serves as a Diagnostic Biomarker for Carotid Artery Stenosis, Forecasting Cerebral Ischemic Events. Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis 1 40692301
2025 NEXN-AS1 Predicts the Occurrence of Post-Stroke Cognitive Impairment and Alleviates Inflammation and Oxidative Stress by Targeting the miR-92a-3p/NRF1 Axis : NEXN-AS1 Alleviates Inflammation and Oxidative Stress in PSCI. Neurochemical research 1 41398486
2026 Nexilin promotes calcium-dependent endo-lysosomal fission required for retrograde transport. Cell communication and signaling : CCS 0 41514273
2025 Nexilin regulates cell surface expression of extrasynaptic GABAA receptors by binding to actin. Neuropharmacology 0 40812511
2025 Transposition of the Great Arteries With Intact Ventricular Septum and Fetal-Onset Dilated Cardiomyopathy in a Neonate With a Homozygous NEXN Variant. Case reports in pediatrics 0 41347213
2025 NEXN targeting MYOCD by facilitating EMT-related β-catenin nuclear translocation modulates the metastasis of hepatocellular carcinoma. iScience 0 41399508
2024 Identification of Novel Nexilin Splice Variants in Mouse and Human Tissues. Cells 0 39682766

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