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Showing SEPTIN2 — NEDD5 is a alias.

SEPTIN2

Septin-2 · UniProt Q15019

Length
361 aa
Mass
41.5 kDa
Annotated
2026-06-10
48 papers in source corpus 23 papers cited in narrative 24 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SEPTIN2 is a GTP-binding cytoskeletal protein that assembles into homotypic and heteromeric filaments to organize membranes and the cytoskeleton across cell division, organelle dynamics, and tissue barrier function (PMID:9203580, PMID:16857012). It binds guanine nucleotides with slow exchange and low hydrolysis rates resembling Ras-family GTPases, and a single SEPTIN2 protein self-assembles into filaments in vitro in either the GDP- or GTP-bound state, with GTP hydrolysis required for assembly of cytoplasmic fibers in cells (PMID:9203580, PMID:16857012). Filament behavior is shaped by specific interaction with PtdIns(4,5)P2, onto which SEPTIN2 adsorbs while preserving its native fold (PMID:23416254). Its best-established role is in cytokinesis: SEPTIN2 accumulates at the contractile ring and condenses into the midbody, and its inhibition produces binucleate cells from cytokinesis failure (PMID:9203580); correct midbody localization and abscission depend on prolyl isomerization of Pro259 by Cyclophilin A (PMID:37446263). As part of SEPT2/6/7 heteromeric filaments it directly controls microtubule plus-end dynamics in a biphasic, concentration-dependent manner and competes with EB1 to trigger its dissociation from plus ends, acting as a barrier to microtubule growth (PMID:31577529). SEPTIN2 also localizes to mitochondrial constriction sites where it directly binds Drp1 and promotes Drp1 recruitment to drive mitochondrial fission (PMID:27215606). At the cell periphery and membranes it supports endothelial podosome maturation and matrix degradation, junctional protein organization, and epithelial barrier function through PtdIns(4,5)P2-dependent mechanisms (PMID:20870893, PMID:31865373, PMID:33147991), and modulates Rac-dependent lamellipodia via interaction with the Rac GAP ARHGAP25 (PMID:40205813). Post-translational control is extensive: casein kinase II phosphorylates Ser218 and Ser248 to alter nucleotide binding (PMID:15150837, PMID:16857012), and S-nitrosylation at Cys111 dissociates SEPTIN2 from TIAM1, releasing the TIAM1-RAC1-NF-κB axis to drive vascular inflammation (PMID:38357802). Mutations in SEPT2 predicted to block homodimerization ablate its interaction with Ankyrin G, causing loss of the axon initial segment and neuronal hypoexcitability, linking SEPTIN2 to human cognitive impairment (PMID:41408595).

Mechanistic history

Synthesis pass · year-by-year structured walk · 21 steps
  1. 1997 High

    Established that SEPTIN2 is a GTP-hydrolysis-dependent cytoskeletal component essential for cytokinesis, defining its foundational cellular role.

    Evidence Inhibitory antibody microinjection, GTPase-deficient mutants, and pharmacological disruption with immunofluorescence in mammalian cells

    PMID:9203580

    Open questions at the time
    • Did not resolve the molecular partners at the contractile ring
    • Mechanism coupling GTP hydrolysis to filament assembly not defined
  2. 2003 Medium

    Linked SEPTIN2 GTPase activity to polarized outgrowth and placed it in proximity to the exocyst and tubulin.

    Evidence Co-immunoprecipitation from brain lysate and GTPase-deficient mutant expression in PC12 cells

    PMID:12544826

    Open questions at the time
    • Co-IP did not establish direct binding to exocyst or tubulin
    • No reciprocal validation
  3. 2004 Medium

    Identified an in vivo phosphorylation site (Ser248) and a candidate kinase, opening a route to post-translational regulation.

    Evidence MALDI-Q-TOF mass spectrometry and S248A mutagenesis on recombinant human SEPT2

    PMID:15150837

    Open questions at the time
    • Functional consequence of Ser248 phosphorylation not tested
    • Kinase identity only predicted
  4. 2006 High

    Quantified SEPTIN2 nucleotide binding and hydrolysis kinetics and showed a single septin can form filaments, biochemically defining it as a slow Ras-like GTPase; tied CK2 phosphorylation at Ser218 to nucleotide binding.

    Evidence In vitro GTP/GDP binding and hydrolysis assays, filament reconstitution with recombinant protein, and in vivo phosphorylation analysis

    PMID:16857012

    Open questions at the time
    • Did not connect filament biochemistry to a specific in-cell structure
    • How phosphorylation regulates assembly in cells unresolved
  5. 2010 Medium

    Extended the cytokinesis role to meiosis and identified SUMO-1 modification, showing septin function is needed for the metaphase I/anaphase I transition.

    Evidence siRNA, pharmacological inhibition, and SUMO modification assays in mouse oocytes

    PMID:20372094

    Open questions at the time
    • SUMO acceptor site and its functional role not defined
    • Inhibitor targets septins broadly, not SEPTIN2 specifically
  6. 2010 Medium

    Demonstrated that membrane redistribution of SEPTIN2 controls epithelial barrier permeability via cortical actin, connecting it to mechanosensing.

    Evidence Immunofluorescence, siRNA depletion, and permeability assays in human airway epithelial cells

    PMID:20870893

    Open questions at the time
    • Molecular trigger for membrane redistribution not identified
    • Direct membrane-binding determinants not tested here
  7. 2011 Medium

    Showed SEPTIN2 has intrinsic amyloid-like self-assembly propensity within its GTP-binding domain, characterizing its biophysical aggregation behavior.

    Evidence In vitro aggregation, Thioflavin-T binding, electron microscopy, and CD spectroscopy

    PMID:21967827

    Open questions at the time
    • Physiological relevance of amyloid-like fibers unknown
    • Relationship to functional septin filaments not established
  8. 2013 Medium

    Identified specific PtdIns(4,5)P2 interaction as a determinant of SEPTIN2 membrane association and fold stability, providing a lipid basis for membrane targeting.

    Evidence Langmuir monolayer assay and PM-IRRAS spectroscopy with recombinant protein

    PMID:23416254

    Open questions at the time
    • Lipid-binding residues not mapped in this study
    • In-cell consequence of the interaction not tested here
  9. 2016 High

    Defined a direct role in mitochondrial fission, showing SEPTIN2 binds Drp1 and is required for Drp1 recruitment to constriction sites.

    Evidence Reciprocal endogenous Co-IP, recombinant protein pulldown, siRNA, and live-cell imaging; replicated in C. elegans

    PMID:27215606

    Open questions at the time
    • Binding interface on SEPTIN2 and Drp1 not mapped
    • Whether GTPase state regulates Drp1 binding unknown
  10. 2016 Medium

    Placed SEPTIN2 downstream of RhoA in fibroblast-to-myofibroblast differentiation and linked it to microtubule acetylation.

    Evidence Adenoviral overexpression, migration assays, RhoA inhibition, and acetyl-α-tubulin quantification

    PMID:27974709

    Open questions at the time
    • Direct effector linking RhoA to SEPTIN2 unidentified
    • Mechanism of tubulin acetylation protection unresolved
  11. 2019 High

    Established SEPT2/6/7 as a direct, biphasic regulator of microtubule plus-end dynamics that competes with EB1, defining a cytoskeletal crosstalk mechanism.

    Evidence In vitro reconstitution of MT dynamics with purified SEPT2/6/7, competitive EB1 binding assays, and live-cell imaging

    PMID:31577529

    Open questions at the time
    • Structural basis of EB1 competition not resolved
    • Concentration thresholds in vivo unknown
  12. 2019 High

    Identified SEPTIN2 cleavage at residue 306 by ZIKV NS2B-NS3 protease as the direct cause of virus-induced cytokinesis defects in neural progenitors.

    Evidence NS2B-NS3 expression with protease-dead controls, non-cleavable SEPTIN2 rescue, Co-IP, and cytokinesis assays

    PMID:30713029

    Open questions at the time
    • Whether other septins are similarly targeted not addressed
    • Fate of cleavage fragments not characterized
  13. 2020 High

    Showed SEPTIN2, with SEPT6/7, is required for endothelial podosome maturation and matrix degradation through its phosphoinositide-binding residues, linking lipid binding to angiogenic invasion.

    Evidence siRNA, PI-binding mutant expression, matrix degradation and invasion assays with live-cell imaging

    PMID:31865373

    Open questions at the time
    • Upstream signals recruiting SEPTIN2 to podosomes unknown
    • Specific lipid species at podosomes not resolved
  14. 2020 Medium

    Demonstrated that junctional SEPTIN2 organizes adherens, tight junction, and PECAM-1 proteins in endothelial monolayers through a membrane-interaction-dependent mechanism.

    Evidence shRNA, PI(4,5)P2-binding mutant overexpression, confocal imaging, immunoblot, and RNA-seq

    PMID:33147991

    Open questions at the time
    • Direct binding to junctional components not shown
    • Mechanism of junctional protein recruitment unresolved
  15. 2022 Medium

    Established SEPTIN2 as the keystone subunit required for septin multimer stability in Schwann cell myelin, ranking it above SEPTIN9 in the assembly hierarchy.

    Evidence Cre/loxP conditional knockout in Schwann cells with Western blot and immunofluorescence of sciatic nerve

    PMID:36378242

    Open questions at the time
    • Mechanism by which SEPTIN2 stabilizes other subunits not defined
    • Functional myelin consequences not fully resolved here
  16. 2023 Medium

    Defined Cyclophilin A-mediated isomerization at Pro259 as a requirement for SEPTIN2 midbody localization and abscission.

    Evidence Co-IP, P259 mutant expression, Cyclophilin A depletion, and abscission assays

    PMID:37446263

    Open questions at the time
    • Structural effect of P259 isomerization not directly resolved
    • Single-lab study without reciprocal validation
  17. 2023 Medium

    Identified a non-cytoskeletal role in ER stress, where SEPTIN2 balances acetylation versus ubiquitination of BiP/GRP78 at Lys327 to constrain macrophage proinflammatory activation.

    Evidence High-content screening, knockdown, Co-IP, and ubiquitination/acetylation and macrophage polarization assays

    PMID:37978190

    Open questions at the time
    • How SEPTIN2 mechanistically arbitrates the two PTMs is unclear
    • Whether this is filament-dependent unknown
  18. 2024 High

    Showed S-nitrosylation at Cys111 acts as a molecular switch dissociating SEPTIN2 from TIAM1 to activate RAC1-NF-κB signaling in vascular inflammation.

    Evidence Biotin-switch with LC-MS/MS, Co-IP, RNA-seq, and pharmacological RAC1 inhibition

    PMID:38357802

    Open questions at the time
    • Nitrosylating source in vivo not pinned down
    • Whether nitrosylation alters filament assembly not tested
  19. 2025 Medium

    Connected SEPTIN2 to axon initial segment maintenance and human cognitive impairment via Ankyrin G binding dependent on SEPTIN2 homodimerization.

    Evidence Co-IP, disease-associated mutant expression in neurons, immunofluorescence, and electrophysiology

    PMID:41408595

    Open questions at the time
    • Genetic causality in patients beyond single-gene assumption not established
    • Binding interface on Ankyrin G not mapped
  20. 2025 Medium

    Identified SEPTIN2 as a regulator of the Rac GAP ARHGAP25 at lamellipodia, modulating Rac-dependent spreading.

    Evidence Co-IP, co-localization, reciprocal siRNA and overexpression epistasis, and lamellipodia/spreading assays

    PMID:40205813

    Open questions at the time
    • Direct binding interface not mapped
    • How SEPTIN2 alters ARHGAP25 GAP activity unresolved
  21. 2025 Medium

    Placed SEPTIN2 in a hypoxia-responsive transcriptional axis controlling spermatogonial proliferation through PP2A-B56γ and AKT.

    Evidence DNA pulldown/MS, TMT proteomics, Co-IP, luciferase reporter, knockdown/overexpression, and PP2A pharmacology

    PMID:40487848

    Open questions at the time
    • Direct interaction of SEPTIN2 with PP2A subunits not fully resolved
    • In vivo relevance to fertility not established

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the diverse post-translational modifications (CK2 phosphorylation, SUMOylation, S-nitrosylation, prolyl isomerization) and lipid interactions are integrated to switch SEPTIN2 between its many filament-dependent and signaling roles remains unresolved.
  • No unified structural model linking nucleotide state, PTMs, and filament assembly
  • Tissue-specific partner selection mechanism unknown
  • Causal hierarchy among SEPTIN2's many roles not established

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0003924 GTPase activity 2 GO:0008289 lipid binding 2 GO:0098772 molecular function regulator activity 2 GO:0008092 cytoskeletal protein binding 1
Localization
GO:0005886 plasma membrane 3 GO:0005829 cytosol 2 GO:0005856 cytoskeleton 2 GO:0005739 mitochondrion 1
Pathway
R-HSA-162582 Signal Transduction 2 R-HSA-1640170 Cell Cycle 2 R-HSA-1852241 Organelle biogenesis and maintenance 1 R-HSA-8953897 Cellular responses to stimuli 1
Partners
ANK3ARHGAP25DRP1EB1FHL2HSPA5PPIATIAM1
Complex memberships
SEPT2/6/7 heteromeric septin complex

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 Nedd5 (SEPTIN2) accumulates near the contractile ring from anaphase through telophase and condenses into the midbody; microinjection of anti-Nedd5 antibody causes cytokinesis failure resulting in binucleated cells, establishing a direct role in cytokinesis. GTP hydrolysis is required for assembly of Nedd5-containing fibers, as shown by GTPγS microinjection and GTP-binding-deficient mutants. Nedd5 fibers physically contact actin bundles and focal adhesion complexes and are disrupted by cytochalasin D, C3 exoenzyme, and serum starvation. Microinjection of inhibitory antibody, expression of GTPase-deficient mutants, pharmacological disruption (cytochalasin D, C3 exoenzyme, GTPγS), immunofluorescence co-localization Genes & development High 9203580
2003 Nedd5 (SEPTIN2) co-immunoprecipitates with the exocyst complex and tubulin from rat brain lysate. Overexpression of a GTPase-defective Nedd5 mutant promotes aberrant neurite sprouting in PC12 cells, indicating that Nedd5 GTPase activity is required for polarized neurite outgrowth. Co-immunoprecipitation from brain lysate, overexpression of GTPase-deficient mutant in PC12 cells, immunofluorescence Neuroreport Medium 12544826
2004 Septin 2 (SEPT2) is phosphorylated in vivo at a single site; mass spectrometric analysis of recombinant human SEPT2 identified Ser248 as the phosphorylation site; site-directed mutagenesis (S248A) abolished phosphorylation. Predicted kinase is casein kinase 2. MALDI-Q-TOF mass spectrometry on purified recombinant protein; site-directed mutagenesis (S248A) Rapid communications in mass spectrometry : RCM Medium 15150837
2006 Recombinant human septin 2 binds guanine nucleotides (Kd 0.28 μM for GTPγS, 1.75 μM for GDP) with slow exchange rates and an apparent kcat of ~2.7×10⁻⁴ s⁻¹, similar to Ras-family GTPases. A single septin 2 protein can form homotypic filaments in vitro bound to either GDP or GTP. Septin 2 is phosphorylated in vivo at Ser218 by casein kinase II, and phosphorylation at Ser218 alters nucleotide binding affinity. In vitro GTP/GDP binding and hydrolysis assays with recombinant protein; in vitro filament reconstitution; in vivo phosphorylation analysis The FEBS journal High 16857012
2010 Septin 2 is modified by SUMO-1 in mouse oocytes during meiotic maturation. Disruption of septin function (inhibitor or siRNA) causes failure of the metaphase I/anaphase I transition and chromosome misalignment, activating the spindle assembly checkpoint (BubR1 labeling). Septin 2 localizes along the meiotic spindle and at the midbody during cytokinesis. siRNA knockdown, pharmacological inhibition, immunofluorescence, SUMO modification assay in mouse oocytes Cell cycle (Georgetown, Tex.) Medium 20372094
2010 Increased membrane localization of septin-2 in airway epithelial cells mediates decreases in paracellular permeability by altering cortical actin arrangement, in response to both physiological shear stress and particulate matter exposure. Septin-2 redistribution to the membrane regulates barrier function and segregates EGF receptor to the basolateral side. Immunofluorescence, siRNA-mediated depletion, permeability assays, actin staining in human airway epithelial cells American journal of respiratory cell and molecular biology Medium 20870893
2011 Human SEPT2 can self-assemble into amyloid-like filaments in vitro. Regions within the GTP-binding domain have propensity to aggregate; temperature-induced unfolding generates a β-sheet-rich intermediate that binds Thioflavin-T and forms micrometer-length fibers visible by electron microscopy. In vitro aggregation assay, Thioflavin-T binding, electron microscopy, CD spectroscopy Biochimie Medium 21967827
2013 SEPT2 preferentially and strongly adsorbs onto PtdIns(4,5)P2 Langmuir monolayers compared to DPPC monolayers, remaining inserted at high surface pressures, while its native secondary structure is preserved only when interacting with PtdIns(4,5)P2. This suggests a specific lipid interaction that may regulate SEPT2 assembly. Langmuir monolayer assay, PM-IRRAS spectroscopy Biochimica et biophysica acta Medium 23416254
2016 Septin 2 localizes to a subset of mitochondrial constriction sites and directly binds Drp1, as shown by immunoprecipitation of endogenous proteins and pulldown with recombinant proteins. Depletion of septin 2 reduces Drp1 recruitment to mitochondria and results in hyperfused mitochondria and delayed FCCP-induced fission. Co-immunoprecipitation of endogenous proteins, pulldown with recombinant proteins, siRNA knockdown, live-cell imaging, immunofluorescence EMBO reports High 27215606
2016 Septin 2 is downstream of RhoA in angiotensin II-stimulated adventitial fibroblast-to-myofibroblast differentiation. Overexpression of septin 2 decreases α-SMA expression and inhibits myofibroblast migration. Septin 2 protects α-tubulin acetylation, and RhoA inhibition decreases myofibroblast motility and septin 2 expression. Adenovirus-mediated gene transfer, Western blot, migration assay, RhoA inhibition, acetyl-α-tubulin quantification Journal of vascular research Medium 27974709
2019 ZIKV protease NS2B-NS3 cleaves Septin-2 at residue 306. Cleavage is protease-activity-dependent, and forced expression of a non-cleavable Septin-2 (R306A or equivalent) restores cytokinesis in NS2B-NS3-expressing cells, establishing Septin-2 cleavage as a direct mechanism of ZIKV-induced cytokinesis defects in neural progenitors. Expression of NS2B-NS3 protease, protease-dead mutant controls, non-cleavable Septin-2 rescue, co-immunoprecipitation, cytokinesis assay Neuron High 30713029
2019 SEPT2/6/7 heteromeric complexes directly regulate microtubule plus-end dynamics in a biphasic, concentration-dependent manner: lower concentrations enhance MT plus-end growth while higher concentrations inhibit or pause it. SEPT2/6/7 competes with EB1 for binding to GTPγS-stabilized microtubules and triggers EB1 dissociation from plus-end tips both in cis (by binding the MT lattice) and in trans (when MT plus ends collide with SEPT2/6/7 filaments). SEPT2/6/7 filaments are more potent barriers than actin filaments for pausing MT growth. In vitro reconstitution of MT dynamics with purified SEPT2/6/7; competitive binding assays with EB1; live-cell imaging Molecular biology of the cell High 31577529
2019 ZIKV NS2B-NS3 protease interacts with Septin-2 (identified among binding partners by co-immunoprecipitation) and cleaves it at residue 306. Co-immunoprecipitation, in vitro cleavage assay, mutant rescue Neuron High 30713029
2020 Septin2, together with Septin6 and Septin7, is required for maturation of nascent endothelial podosomes into matrix-degrading organelles. Septin2 localizes around the perimeter of podosomes near the basolateral plasma membrane. Phosphoinositide-binding residues of Septin2 are required for podosome function, and Septin2-mediated podosome regulation is critical for endothelial cell invasion associated with angiogenesis. siRNA knockdown of Septin2/6/7, live-cell imaging, immunofluorescence, PI-binding mutant expression, matrix degradation assay, in vitro invasion assay The Journal of cell biology High 31865373
2020 Junctional localization of Septin 2 is required for the organization of adherens junction proteins (nectin-2, afadin), tight junction protein ZO-1, and PECAM-1 in endothelial monolayers. Loss of junctional Septin 2 (via shRNA, TNF-α treatment, or PI(4,5)P2-binding mutant overexpression) disorganizes these junctional proteins, implicating a plasma membrane-interaction-dependent mechanism. shRNA knockdown, PI(4,5)P2-binding mutant overexpression, immunofluorescence confocal imaging, immunoblot, RNA-seq Arteriosclerosis, thrombosis, and vascular biology Medium 33147991
2022 SEPTIN2 is required for the formation or stabilization of the septin multimer in peripheral nervous system (Schwann cell) myelin: deletion of Septin2 in Schwann cells markedly reduced abundance of all relevant septin subunits (SEPT7, SEPT8, SEPT9, SEPT11), whereas Septin9 deletion had no effect on other septin subunits. Cre/loxP conditional knockout in Schwann cells, Western blot of sciatic nerve, immunofluorescence Cytoskeleton (Hoboken, N.J.) Medium 36378242
2023 Cyclophilin A isomerizes Septin 2 at proline 259 and this interaction is required for Septin 2 localization to the midbody and for abscission during cytokinesis. Cyclophilin A depletion or loss of isomerase activity induces Septin 2 defects at the midbody. A Septin 2 P259 mutant (isomerization-deficient) shows defective midbody localization and impaired abscission. Co-immunoprecipitation, structural/molecular analysis of P259, EGFP-Septin2 mutant expression, Cyclophilin A depletion, abscission assay International journal of molecular sciences Medium 37446263
2023 ER stress induces SEPT2 expression; SEPT2 balances the competition between acetylation and ubiquitination of heat shock protein 5 (BiP/GRP78) at Lysine 327, thereby alleviating ER stress and constraining M1-like macrophage polarization and proinflammatory cytokine release. This constitutes a negative feedback loop suppressing IFN-γ-independent macrophage autoactivation. High-content screening, siRNA/shRNA knockdown, co-immunoprecipitation, ubiquitination and acetylation assays, macrophage polarization assays Nature communications Medium 37978190
2024 Septin2 is S-nitrosylated at Cys111 (SNO-Septin2) in aortic tissue. Unmodified Septin2 interacts with TIAM1; SNO-Septin2 reduces this interaction, releasing TIAM1 to activate the TIAM1-RAC1 axis and downstream NF-κB signaling, resulting in macrophage-mediated vascular inflammation and extracellular matrix degradation that drives aortic aneurysm and dissection. Biotin-switch assay + LC-MS/MS to identify S-nitrosylation site, co-immunoprecipitation to detect TIAM1 interaction, RNA-seq, pharmacological inhibition of RAC1 Circulation High 38357802
2025 Septin-2 binds a neuron-specific domain of Ankyrin G, and this interaction is required for maintenance of the axon initial segment (AIS). Mutations in SEPT2 (found in humans with cognitive impairment) predicted to block Septin-2 homodimerization largely ablate Ankyrin G interaction; expression of mutant Septin-2 in neurons causes loss of Ankyrin G from the AIS, aberrant MAP2 localization in the distal axon, axonal shortening, and electrophysiological hypoexcitability. Co-immunoprecipitation of SEPT2 with Ankyrin G, expression of disease-associated mutants in neurons, immunofluorescence, electrophysiology Brain : a journal of neurology Medium 41408595
2025 Septin2 regulates ARHGAP25 (a Rac GAP) function at lamellipodia: SEPTIN2 interacts with ARHGAP25 and co-localizes at lamellipodia. Overexpression of ARHGAP25 suppresses Rac-dependent lamellipodia formation and cell spreading, and this suppression is restored by Septin2 depletion. Conversely, Septin2 overexpression enhances ARHGAP25-mediated suppression in an ARHGAP25-dependent manner. Co-immunoprecipitation, immunofluorescence co-localization, siRNA knockdown, overexpression, lamellipodia and cell spreading assays FEBS letters Medium 40205813
2025 Hypoxia suppresses Septin2 (SEPT2) transcription in spermatogonia by interfering with POLR2A binding to the Sept2 promoter. Reduced SEPT2 stabilizes the B56γ regulatory subunit of PP2A, enhancing AKT dephosphorylation and causing G1-S phase arrest that impairs spermatogonial proliferation. PP2A inhibitor (okadaic acid) rescues hypoxia-induced proliferative defects. DNA pulldown/mass spectrometry (identifying POLR2A), TMT-based quantitative proteomics, co-immunoprecipitation, dual-luciferase reporter, siRNA knockdown and overexpression, pharmacological PP2A inhibition/activation Human reproduction open Medium 40487848
2018 CDK2 interacts with SEPT2 and stabilizes SEPT2 protein in hepatocellular carcinoma cells. Both CDK2 and SEPT2 show peak protein levels at G2/M phase. Co-immunoprecipitation, Western blot across cell cycle phases Cancer science Low 30444001
2014 FHL2 is an interaction protein of Septin2 in mesangial cells. Septin2 knockdown induces apoptosis through FHL2-mediated signaling involving p-ERK1 and p-AKT pathways; this apoptosis is alleviated by FHL2 overexpression. Co-immunoprecipitation, siRNA knockdown, label-free quantitative proteomics, Western blot Proteomics Low 25103794

Source papers

Stage 0 corpus · 48 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1997 Nedd5, a mammalian septin, is a novel cytoskeletal component interacting with actin-based structures. Genes & development 327 9203580
2016 A role for septin 2 in Drp1-mediated mitochondrial fission. EMBO reports 81 27215606
2019 Zika Virus Protease Cleavage of Host Protein Septin-2 Mediates Mitotic Defects in Neural Progenitors. Neuron 67 30713029
2003 The septin protein Nedd5 associates with both the exocyst complex and microtubules and disruption of its GTPase activity promotes aberrant neurite sprouting in PC12 cells. Neuroreport 65 12544826
2006 GTP binding and hydrolysis kinetics of human septin 2. The FEBS journal 54 16857012
2006 Proteomic identification of a role for the von Hippel Lindau tumour suppressor in changes in the expression of mitochondrial proteins and septin 2 in renal cell carcinoma. Proteomics 53 16739133
2023 SEPTIN2 suppresses an IFN-γ-independent, proinflammatory macrophage activation pathway. Nature communications 47 37978190
2010 Septin-2 mediates airway epithelial barrier function in physiologic and pathologic conditions. American journal of respiratory cell and molecular biology 47 20870893
2024 S-Nitrosylation of Septin2 Exacerbates Aortic Aneurysm and Dissection by Coupling the TIAM1-RAC1 Axis in Macrophages. Circulation 42 38357802
2018 Repression of Septin9 and Septin2 suppresses tumor growth of human glioblastoma cells. Cell death & disease 41 29724999
2005 Screening of an endothelial cDNA library identifies the C-terminal region of Nedd5 as a novel autoantigen in systemic lupus erythematosus with psychiatric manifestations. Arthritis research & therapy 41 15987492
2020 Extracellular vesicle-derived circ_SLC19A1 promotes prostate cancer cell growth and invasion through the miR-497/septin 2 pathway. Cell biology international 37 31903637
2016 Septin 2 accelerates the progression of biliary tract cancer and is negatively regulated by mir-140-5p. Gene 33 27155525
2019 Septin 2/6/7 complexes tune microtubule plus-end growth and EB1 binding in a concentration- and filament-dependent manner. Molecular biology of the cell 28 31577529
2011 Self assembly of human septin 2 into amyloid filaments. Biochimie 27 21967827
2002 Expression of Nedd5, a mammalian septin, in human brain tumors. Journal of neuro-oncology 27 12125979
2021 The LncRNA FGD5-AS1/miR-497-5p axis regulates septin 2 (SEPT2) to accelerate cancer progression and increase cisplatin-resistance in laryngeal squamous cell carcinoma. Molecular carcinogenesis 25 34003510
2023 Lysyl hydroxylase LH1 promotes confined migration and metastasis of cancer cells by stabilizing Septin2 to enhance actin network. Molecular cancer 23 36721170
2010 Septin2 is modified by SUMOylation and required for chromosome congression in mouse oocytes. Cell cycle (Georgetown, Tex.) 23 20372094
2004 Septin 2 phosphorylation: theoretical and mass spectrometric evidence for the existence of a single phosphorylation site in vivo. Rapid communications in mass spectrometry : RCM 21 15150837
2020 Junctional Localization of Septin 2 Is Required for Organization of Junctional Proteins in Static Endothelial Monolayers. Arteriosclerosis, thrombosis, and vascular biology 17 33147991
2020 Septin2 mediates podosome maturation and endothelial cell invasion associated with angiogenesis. The Journal of cell biology 14 31865373
2022 Human umbilical cord-mesenchymal stem cells-derived exosomes carrying microRNA-15a-5p possess therapeutic effects on Wilms tumor via regulating septin 2. Bioengineered 13 35200105
2018 An invertebrate β-integrin mediates coelomocyte phagocytosis via activation of septin2 and 7 but not septin10. International journal of biological macromolecules 13 29526576
2018 Coupling function of cyclin-dependent kinase 2 and Septin2 in the promotion of hepatocellular carcinoma. Cancer science 10 30444001
2013 Lipid interaction triggering Septin2 to assembly into β-sheet structures investigated by Langmuir monolayers and PM-IRRAS. Biochimica et biophysica acta 10 23416254
2019 Septin-2 is overexpressed in epithelial ovarian cancer and mediates proliferation via regulation of cellular metabolic proteins. Oncotarget 8 31105878
2019 Expression of septin 2 and association with clinicopathological parameters in colorectal cancer. Oncology letters 8 31402940
2016 Adenovirus-Mediated Overexpression of Septin 2 Attenuates α-Smooth Muscle Actin Expression and Adventitial Myofibroblast Migration Induced by Angiotensin II. Journal of vascular research 8 27974709
2015 SEPTIN2 and STATHMIN Regulate CD99-Mediated Cellular Differentiation in Hodgkin's Lymphoma. PloS one 7 26000982
2022 Molecular characterization and expression analysis of septin gene family and phagocytic function of recombinant septin 2, 3 and 8 of starry flounder (Platichthys stellatus). Fish & shellfish immunology 5 35577319
2019 Glucose starvation triggers filamentous septin assemblies in an S. pombe septin-2 deletion mutant. Biology open 5 30602528
2018 MicroRNA-223 Regulates Septin-2 and Septin-6 in Stored Platelets. MicroRNA (Shariqah, United Arab Emirates) 5 29943706
2014 FHL2-driven molecular network mediated Septin2 knockdown inducing apoptosis in mesangial cell. Proteomics 5 25103794
2024 Molecular insights into septin 2 protein in rohu (Labeo rohita): revealing expression dynamics, antimicrobial activity and functional characteristics. International journal of biological macromolecules 4 39743099
2022 Septin 2 interacts with dengue virus replication complex proteins and participates in virus replication in mosquito cells. Virology 4 35390695
2022 Targeted inactivation of the Septin2 and Septin9 genes in myelinating Schwann cells of mice. Cytoskeleton (Hoboken, N.J.) 4 36378242
2017 [Expression of Septin2 in Hodgkin lymphoma cell line L428 and its role in promoting H/RS cells' redifferentiation to B lymphocytes]. Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi 4 28279038
2007 A cascade involving p85, Cdc42 and septin 2 regulates cytokinesis. Biochemical Society transactions 4 17371243
2025 Hypoxia exposure impairs male fertility via inhibiting Septin2-mediated spermatogonial proliferation. Human reproduction open 2 40487848
2025 Forchlorfenuron exposure induces cardiotoxicity via NF-κB/NLRP3-mediated inflammasome activation independent of Septin2 inhibition. Free radical biology & medicine 1 41412525
2021 Expression of Septin 2 and Her2/neu in Colorectal Cancer. Journal of microscopy and ultrastructure 1 36687331
2025 Septin2 regulates ARHGAP25-mediated suppression of lamellipodia formation and cell spreading. FEBS letters 0 40205813
2025 A functional role for septin-2 in the maintenance of the axon initial segment and in human cognitive development. Brain : a journal of neurology 0 41408595
2025 Septin 2: A direct target of Ginsenoside Rg1 mediating its neuroprotective effects. Biochemical pharmacology 0 41421452
2024 The S-Nitrosylation of Septin2 (SNO-Septin2) axis: A novel potential therapeutic target for treating aneurysms and dissection. Drug discoveries & therapeutics 0 38987209
2023 Cyclophilin A Isomerisation of Septin 2 Mediates Abscission during Cytokinesis. International journal of molecular sciences 0 37446263
2007 [An observation of the effects of the truncated septin2 on mouse epidermal cell and fibroblast]. Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery 0 17554884

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