Affinage

NDUFA3

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 · UniProt O95167

Round 2 corrected
Length
84 aa
Mass
9.3 kDa
Annotated
2026-04-29
130 papers in source corpus 9 papers cited in narrative 9 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NDUFA3 is a nuclear-encoded accessory subunit of mitochondrial NADH:ubiquinone oxidoreductase (complex I) that resides in the inner mitochondrial membrane and is essential for holoenzyme assembly and activity. miRNA knockdown and CRISPR/Cas9 knockout studies demonstrate that NDUFA3 is specifically required for assembly and stability of the Q (ubiquinone-binding) module in the peripheral arm of complex I; its absence destabilizes co-module subunits and abolishes complex I function (PMID:24717771, PMID:27626371). Cryo-EM structural analysis of the human respiratory megacomplex I₂III₂IV₂ places NDUFA3 at a defined position within the complex I architecture, and phosphoproteomics identifies it as a substrate of reversible phosphorylation in human skeletal muscle mitochondria (PMID:28844695, PMID:20833797).

Mechanistic history

Synthesis pass · year-by-year structured walk · 8 steps
  1. 1998 Medium

    Identification of the NDUFA3 cDNA sequence completed the molecular catalogue of human complex I subunits, establishing it as one of the last nuclear-encoded subunits to be characterized at the sequence level.

    Evidence cDNA cloning and sequencing of previously uncharacterized complex I subunits

    PMID:9878551

    Open questions at the time
    • No functional data beyond sequence identification
    • Subcellular localization inferred but not directly demonstrated
  2. 2003 High

    Biochemical isolation of intact complex I confirmed that NDUFA3 is a bona fide physical component of the holoenzyme, resolving whether it is a true stoichiometric subunit versus a copurifying contaminant.

    Evidence Rapid immunopurification of human complex I with 1D/2D gel electrophoresis, MALDI-TOF, and LC-MS/MS identification

    PMID:12611891

    Open questions at the time
    • Position within complex I architecture unknown
    • Functional role in complex I activity or assembly not tested
  3. 2010 Medium

    Detection of phosphorylation sites on NDUFA3 in human muscle mitochondria raised the possibility that post-translational modification regulates complex I function through this accessory subunit.

    Evidence Phosphoproteomics of isolated human skeletal muscle mitochondria with multiple enrichment strategies and LC-MS/MS

    PMID:20833797

    Open questions at the time
    • Responsible kinase(s) and phosphatase(s) not identified
    • Functional consequence of phosphorylation on complex I activity not determined
    • Single tissue type examined
  4. 2014 High

    Loss-of-function experiments established that NDUFA3 is not merely structural but is specifically required for assembly of the Q module in the peripheral arm, defining its mechanistic role in complex I biogenesis.

    Evidence miRNA knockdown in human cell lines with BN-PAGE analysis of assembly intermediates and complex I activity assays

    PMID:24717771

    Open questions at the time
    • Knockdown may retain residual protein; complete ablation not yet shown
    • Molecular contacts mediating Q-module scaffolding role undefined
  5. 2016 High

    CRISPR knockout confirmed the essential and non-redundant role of NDUFA3 in complex I assembly and revealed that its loss destabilizes specific co-module subunits, strengthening the model of module-specific scaffolding.

    Evidence CRISPR/Cas9 knockout in human cells with TMT quantitative proteomics, BN-PAGE, and activity assays

    PMID:27626371

    Open questions at the time
    • Direct binding partners within the Q module not mapped at residue resolution
    • Whether NDUFA3 loss affects supercomplex (respirasome) formation beyond complex I not fully dissected
  6. 2017 High

    Cryo-EM structure of the human respiratory megacomplex provided the first near-atomic view of NDUFA3 within complex I, resolving its spatial position at the interface of structural modules.

    Evidence Cryo-electron microscopy of human mitochondrial megacomplex I₂III₂IV₂

    PMID:28844695

    Open questions at the time
    • Structural basis for how NDUFA3 loss specifically collapses Q-module assembly not mechanistically resolved
    • Phosphorylation sites not resolved in the structure
  7. 2019 Medium

    Co-fractionation interactome mapping showed that NDUFA3 interaction networks remodel during neuronal differentiation, suggesting its complex I context is dynamically regulated in a cell-state-dependent manner.

    Evidence Co-fractionation mass spectrometry in human pluripotent stem cells and neuronal-like cells

    PMID:31536960

    Open questions at the time
    • Co-fractionation provides indirect evidence of interaction; direct binding not confirmed
    • Functional significance of interaction remodeling for respirasome dynamics not tested
  8. 2021 Medium

    Quantitative mitochondrial proteome profiling defined NDUFA3 abundance and protein half-life, establishing baseline parameters for understanding its stoichiometry and turnover within complex I.

    Evidence Quantitative proteomics with dynamic SILAC in human cells

    PMID:34800366

    Open questions at the time
    • No mechanistic insight beyond quantitative parameters
    • Half-life measurements from cultured cells may not reflect tissue-specific turnover

Open questions

Synthesis pass · forward-looking unresolved questions
  • The functional consequences of NDUFA3 phosphorylation on complex I assembly and activity remain unknown, and no human disease mutations in NDUFA3 have been reported to directly cause mitochondrial complex I deficiency.
  • No patient mutations in NDUFA3 linked to mitochondrial disease
  • Kinase/phosphatase regulatory circuit acting on NDUFA3 uncharacterized
  • Structural mechanism by which NDUFA3 scaffolds Q-module assembly at residue-level resolution not elucidated

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 2
Localization
GO:0005739 mitochondrion 4
Pathway
R-HSA-1430728 Metabolism 4
Complex memberships
Mitochondrial complex I (NADH:ubiquinone oxidoreductase)Respiratory megacomplex I2III2IV2

Evidence

Reading pass · 9 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 The cDNA sequence of NDUFA3 (a nuclear-encoded subunit located in the hydrophobic protein fraction of complex I) was characterized, completing the catalogue of all then-known 41 human NADH:ubiquinone oxidoreductase (complex I) subunit sequences. NDUFA3 was identified as one of eight previously uncharacterized nuclear-encoded subunits of human complex I. cDNA cloning and sequencing Biochemical and biophysical research communications Medium 9878551
2003 NDUFA3 was identified as a bona fide subunit of immunopurified human NADH dehydrogenase (complex I) by one-dimensional and two-dimensional gel electrophoresis combined with MALDI-TOF peptide mass fingerprinting and LC-MS/MS, establishing its physical presence in the intact human complex I holoenzyme. Rapid immunopurification of complex I followed by 1D/2D gel electrophoresis, MALDI-TOF, and LC-MS/MS The Journal of biological chemistry High 12611891
2010 NDUFA3 was detected as a mitochondrial phosphoprotein in human skeletal muscle, with phosphorylation sites identified by phosphoproteomics, indicating that NDUFA3 undergoes reversible phosphorylation and is a potential substrate for mitochondrial kinases including protein kinase A. Phosphoproteomics of isolated human muscle mitochondria using multiple phosphopeptide enrichment techniques and LC-MS/MS Molecular & cellular proteomics : MCP Medium 20833797
2014 Knockdown of NDUFA3 (along with NDUFA5 and NDUFA12) using miRNA in human cell lines demonstrated that NDUFA3 is required for formation of a functional complex I holoenzyme. Analysis of assembly intermediates revealed that NDUFA3 is specifically needed for assembly and/or stability of the electron-transferring Q module in the peripheral (extramembrane) arm of complex I, rather than the membrane arm. miRNA-mediated knockdown in human cell lines, BN-PAGE analysis of assembly intermediates, complex I activity assays FEBS letters High 24717771
2016 CRISPR/Cas9 knockout of NDUFA3 in human cells confirmed it is strictly required for assembly of a functional complex I. Quantitative proteomics of the knockout showed that loss of NDUFA3 destabilizes other subunits residing in the same structural module, demonstrating a module-specific scaffolding role for NDUFA3 in complex I biogenesis. CRISPR/Cas9 gene editing, quantitative proteomics (TMT mass spectrometry), BN-PAGE, complex I activity assays Nature High 27626371
2017 Cryo-EM structure of the human respiratory megacomplex I2III2IV2 enabled precise assignment of NDUFA3 as a subunit within complex I, contributing to the structural understanding of how NDUFA3 is positioned within the intact supercomplex assembly at the interface of complex I modules. Cryo-electron microscopy of human mitochondrial megacomplex I2III2IV2 Cell High 28844695
2019 NDUFA3 was identified as a component of the mitochondrial interactome in human pluripotent stem cells and neuronal-like cells by co-fractionation mass spectrometry, and its interaction network was shown to remodel during neuronal differentiation, linking NDUFA3 interactions to respirasome dynamics. Mass spectrometry-based co-fractionation profiling (mitochondrial interactome mapping) in two human cell states iScience Medium 31536960
2021 NDUFA3 was confirmed as a member of the high-confidence human mitochondrial proteome (MitoCoP) with quantified abundance and protein half-life, establishing it as a stably expressed mitochondrial inner membrane complex I component with defined turnover dynamics in human cells. Quantitative proteomics of mitochondrial fractions from human cells combined with dynamic SILAC for half-life measurements Cell metabolism Medium 34800366
2022 Endogenous GFP tagging and live-cell confocal imaging in the OpenCell project confirmed that NDUFA3 localizes to mitochondria in human cells, consistent with its role as a complex I subunit in the inner mitochondrial membrane. Endogenous GFP tagging via genome engineering, confocal live-cell imaging, affinity purification-mass spectrometry Science (New York, N.Y.) Medium 35271311

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2016 Accessory subunits are integral for assembly and function of human mitochondrial complex I. Nature 450 27626371
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2017 Architecture of Human Mitochondrial Respiratory Megacomplex I2III2IV2. Cell 391 28844695
2021 Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context. Cell metabolism 239 34800366
2016 Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function. Molecular cell 220 27499296
2014 Conditional anterograde tracing reveals distinct targeting of individual serotonin cell groups (B5-B9) to the forebrain and brainstem. Brain structure & function 219 25403254
2016 An organelle-specific protein landscape identifies novel diseases and molecular mechanisms. Nature communications 211 27173435
2018 Assembly of mammalian oxidative phosphorylation complexes I-V and supercomplexes. Essays in biochemistry 206 30030361
2011 Folate (vitamin B9) and vitamin B12 and their function in the maintenance of nuclear and mitochondrial genome integrity. Mutation research 201 22093367
2014 Global mapping of herpesvirus-host protein complexes reveals a transcription strategy for late genes. Molecular cell 173 25544563
2000 Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. Genome research 161 11042152
2017 Prostate-specific membrane antigen cleavage of vitamin B9 stimulates oncogenic signaling through metabotropic glutamate receptors. The Journal of experimental medicine 142 29141866
2010 Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes. Molecular & cellular proteomics : MCP 138 20833797
2005 Rab11a and myosin Vb are required for bile canalicular formation in WIF-B9 cells. Proceedings of the National Academy of Sciences of the United States of America 129 16214890
2011 Disruption of a ciliary B9 protein complex causes Meckel syndrome. American journal of human genetics 120 21763481
2012 A pivotal role of vitamin B9 in the maintenance of regulatory T cells in vitro and in vivo. PloS one 100 22363800
2003 The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification. The Journal of biological chemistry 90 12611891
2008 Functional redundancy of the B9 proteins and nephrocystins in Caenorhabditis elegans ciliogenesis. Molecular biology of the cell 88 18337471
2011 Homeobox B9 induces epithelial-to-mesenchymal transition-associated radioresistance by accelerating DNA damage responses. Proceedings of the National Academy of Sciences of the United States of America 81 21930940
2017 A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III. Cell metabolism 78 28380382
2008 Oxidative stress, telomere length and biomarkers of physical aging in a cohort aged 79 years from the 1932 Scottish Mental Survey. Mechanisms of ageing and development 76 18977241
2014 A genetically attenuated malaria vaccine candidate based on P. falciparum b9/slarp gene-deficient sporozoites. eLife 72 25407681
2013 TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex. Molecular and cellular biology 72 24344204
2001 Peptide and major histocompatibility complex-specific breaking of humoral tolerance to native insulin with the B9-23 peptide in diabetes-prone and normal mice. Diabetes 67 11375327
1997 Distribution, quantification, and morphological characteristics of serotonin-immunoreactive cells of the supralemniscal nucleus (B9) and pontomesencephalic reticular formation in the rat. The Journal of comparative neurology 66 9034900
1998 cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed. Biochemical and biophysical research communications 61 9878551
1996 Establishment of hepatic cell polarity in the rat hepatoma-human fibroblast hybrid WIF-B9. A biphasic phenomenon going from a simple epithelial polarized phenotype to an hepatic polarized one. Journal of cell science 61 8799849
2007 A genetic association analysis of cognitive ability and cognitive ageing using 325 markers for 109 genes associated with oxidative stress or cognition. BMC genetics 60 17601350
2014 Supernumerary subunits NDUFA3, NDUFA5 and NDUFA12 are required for the formation of the extramembrane arm of human mitochondrial complex I. FEBS letters 59 24717771
1984 Kappa-chain allotypes and isotypes in the rabbit: cDNA sequences of clones encoding b9 suggest an evolutionary pathway and possible role of the interdomain disulfide bond in quantitative allotype expression. Proceedings of the National Academy of Sciences of the United States of America 59 6424124
1982 Structural study of hemoglobin Knossos, beta 27 (B9) Ala leads to Ser. A new abnormal hemoglobin present as a silent beta-thalassemia. FEBS letters 51 7173395
2018 Inoculation of soil with cadmium-resistant bacterium Delftia sp. B9 reduces cadmium accumulation in rice (Oryza sativa L.) grains. Ecotoxicology and environmental safety 50 30055387
2019 Rewiring of the Human Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of the Respirasome and Neurogenesis. iScience 45 31536960
1980 Adaptation of Alcaligenes eutrophus B9 and Pseudomonas sp. B13 to 2-Fluorobenzoate as Growth Substrate. Applied and environmental microbiology 44 16345497
2003 Investigation of the role of a beta(1-4) agarase produced by Pseudoalteromonas gracilis B9 in eliciting disease symptoms in the red alga Gracilaria gracilis. Microbiology (Reading, England) 42 14523124
2019 Anti-Tumor Effects of Vitamin B2, B6 and B9 in Promonocytic Lymphoma Cells. International journal of molecular sciences 41 31374832
2014 Quorum sensing in marine snow and its possible influence on production of extracellular hydrolytic enzymes in marine snow bacterium Pantoea ananatis B9. FEMS microbiology ecology 39 25764555
2020 Enhancement of phenol biodegradation: Metabolic division of labor in co-culture of Stenotrophomonas sp. N5 and Advenella sp. B9. Journal of hazardous materials 38 32585516
2017 Homeobox B9 Mediates Resistance to Anti-VEGF Therapy in Colorectal Cancer Patients. Clinical cancer research : an official journal of the American Association for Cancer Research 38 28298545
1998 Efficient in vitro vectorial transport of a fluorescent conjugated bile acid analogue by polarized hepatic hybrid WIF-B and WIF-B9 cells. Hepatology (Baltimore, Md.) 38 9462660
2010 Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. PloS one 37 20877624
2019 The concomitant lower concentrations of vitamins B6, B9 and B12 may cause methylation deficiency in autistic children. The Journal of nutritional biochemistry 36 31151052
2016 Divergent evolution of vitamin B9 binding underlies Juno-mediated adhesion of mammalian gametes. Current biology : CB 34 26859261
2014 Efficacy of Acinetobacter sp. B9 for simultaneous removal of phenol and hexavalent chromium from co-contaminated system. Applied microbiology and biotechnology 32 25062955
2002 Mechanism by which cAMP activates PI3-kinase and increases bile acid secretion in WIF-B9 cells. American journal of physiology. Cell physiology 31 12388099
2001 Spatiotemporal expression of catenins, ZO-1, and occludin during early polarization of hepatic WIF-B9 cells. American journal of physiology. Cell physiology 30 11171572
2004 Human/mouse cross-reactive anti-VEGF receptor 2 recombinant antibodies selected from an immune b9 allotype rabbit antibody library. Journal of immunological methods 29 15183093
1986 Homozygous hemoglobin Knossos (alpha 2 beta 227(B9) Ala----Ser): a new variety of beta (+)-thalassemia intermedia associated with delta (0)-thalassemia. Blood 29 3955238
2021 Encapsulation of folic acid (vitamin B9) into sporopollenin microcapsules: Physico-chemical characterisation, in vitro controlled release and photoprotection study. Materials science & engineering. C, Materials for biological applications 28 34474830
2020 Formation of the B9-domain protein complex MKS1-B9D2-B9D1 is essential as a diffusion barrier for ciliary membrane proteins. Molecular biology of the cell 26 32726168
2011 Complete genome sequence of Thermomonospora curvata type strain (B9). Standards in genomic sciences 26 21475583
1999 Structure of an insulin dimer in an orthorhombic crystal: the structure analysis of a human insulin mutant (B9 Ser-->Glu). Acta crystallographica. Section D, Biological crystallography 26 10489447
2017 Effect of B9 and B12 vitamin intake on semen parameters and fertility of men with MTHFR polymorphisms. Andrology 25 28440964
1991 Proton nuclear magnetic resonance study of the B9(Asp) mutant of human insulin. Sequential assignment and secondary structure. Journal of molecular biology 25 2002506
2014 Bevacizumab terminates homeobox B9-induced tumor proliferation by silencing microenvironmental communication. Molecular cancer 24 24885802
1993 Protein tyrosine phosphorylation and activation of primary response genes by interleukin 11 in B9-TY1 cells. Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 24 8398901
2020 Homeobox B9 integrates bone morphogenic protein 4 with inflammation at atheroprone sites. Cardiovascular research 23 31504243
2018 Fungal Competitors Affect Production of Antimicrobial Lipopeptides in Bacillus subtilis Strain B9-5. Journal of chemical ecology 23 29492723
2016 Citric acid production from partly deproteinized whey under non-sterile culture conditions using immobilized cells of lactose-positive and cold-adapted Yarrowia lipolytica B9. Journal of biotechnology 23 27234881
2008 Knockdown of tight junction protein claudin-2 prevents bile canalicular formation in WIF-B9 cells. Histochemistry and cell biology 23 19084987
1994 Stimulation of the B9 hybridoma cell line by soluble interleukin-6 receptors. Journal of immunological methods 23 8046256
2022 Simplifying the B Complex: How Vitamins B6 and B9 Modulate One Carbon Metabolism in Cancer and Beyond. Metabolites 22 36295863
2006 Investigation of the hepatotoxicity profile of chemical entities using Liverbeads and WIF-B9 in vitro models. Toxicology in vitro : an international journal published in association with BIBRA 20 16504461
2021 A novel p53 regulator, C16ORF72/TAPR1, buffers against telomerase inhibition. Aging cell 19 33660365
2000 The analysis of the complement activation product SC5 b-9 is applicable in neonates in spite of their profound C9 deficiency. Journal of perinatal medicine 19 10765513
2023 Serpin B9 controls tumor cell killing by CAR T cells. Journal for immunotherapy of cancer 18 36931661
2015 Accurate measurement of the essential micronutrients methionine, homocysteine, vitamins B6, B12, B9 and their metabolites in plasma, brain and maternal milk of mice using LC/MS ion trap analysis. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 18 26197436
2012 Design of human granzyme B variants resistant to serpin B9. Proteins 18 22733450
1989 Beta-thalassemia intermedia in two Turkish families is caused by the interaction of Hb Knossos [beta 27(B9)Ala----Ser] and of Hb City of Hope [beta 69(E13)Gly----ser] with beta (0)-thalassemia. Hemoglobin 18 2467892
2018 Protocadherin B9 promotes resistance to bicalutamide and is associated with the survival of prostate cancer patients. The Prostate 17 30324761
2014 Overexpressed homeobox B9 regulates oncogenic activities by transforming growth factor-β1 in gliomas. Biochemical and biophysical research communications 17 24582746
2011 Annexin B9 binds to β(H)-spectrin and is required for multivesicular body function in Drosophila. Journal of cell science 17 21878499
1994 Oncostatin M stimulates proliferation in B9 hybridoma cells: potential role of oncostatin M in plasmacytoma development. Cytokine 17 8031997
2010 Two new alpha1-globin gene point mutations: Hb Nedlands (HBA1:c.86C>T) [alpha28(B9)Ala-->Val] and Hb Queens Park (HBA1:c.98T>A) [alpha32(B13)Met-->Lys]. Hemoglobin 16 20353346
2013 Cadmium disorganises the scaffolding of gap and tight junction proteins in the hepatic cell line WIF B9. Biology of the cell 15 24117459
2012 Silencing of homeobox B9 is associated with down-regulation of CD56 and extrathyroidal extension of tumor in papillary thyroid carcinoma. Human pathology 15 22225776
2010 Single nucleotide polymorphisms of matrix metalloproteinase 9 (MMP9) and tumor protein 73 (TP73) interact with Epstein-Barr virus in chronic lymphocytic leukemia: results from the European case-control study EpiLymph. Haematologica 15 21048031
1998 Modulation of homeobox B6 and B9 genes expression in human leukemia cell lines during myelomonocytic differentiation. Leukemia & lymphoma 15 9922051
2022 The Metabolites of Lactobacillus fermentum F-B9-1 Relieved Dextran Sulfate Sodium-Induced Experimental Ulcerative Colitis in Mice. Frontiers in microbiology 14 35572623
2015 The C677T MTHFR genotypes influence the efficacy of B9 and B12 vitamins supplementation to lowering plasma total homocysteine in hemodialysis. Journal of nephrology 14 26559681
1975 Synaptosomal uptake and levels of serotonin in rat brain areas after p-chloroamphetamine or B-9 lesions. European journal of pharmacology 14 1102320
2023 Identification of two novel heterodimeric ABC transporters in melanoma: ABCB5β/B6 and ABCB5β/B9. The Journal of biological chemistry 12 38145744
2017 Unmethylated promoter DNA correlates with p53 expression and apoptotic levels only in Vitamin B9 and B12 deficient megaloblastic anemia but not in non-megaloblastic anemia controls. International journal of biological macromolecules 12 29246873
1975 Sequence variability of rabbit antibody light chains. Familial occurrence of n-terminal sequence differences between b4 and b9 light chains. Scandinavian journal of immunology 12 806962
2022 Neuroprotective Effects of Lycium Barbarum Fruit Extract on Pink1B9Drosophila Melanogaster Genetic Model of Parkinson's Disease. Plant foods for human nutrition (Dordrecht, Netherlands) 11 36322321
2021 Insights into homeobox B9: a propeller for metastasis in dormant prostate cancer progenitor cells. British journal of cancer 11 34247196
2019 A blend containing docosahexaenoic acid, arachidonic acid, vitamin B12, vitamin B9, iron and sphingomyelin promotes myelination in an in vitro model. Nutritional neuroscience 11 30806182
2001 Predominance of human versus rat phenotype in the metabolic pathways for bile acid synthesis by hybrid WIF-B9 cells. Biochimica et biophysica acta 11 11750886
1978 Variable region correlates of group b allotypes: amino acid sequence studies of b9 L chains from homogeneous antibodies. European journal of immunology 11 97088
2017 Paleomicrobiology to investigate copper resistance in bacteria: isolation and description of Cupriavidus necator B9 in the soil of a medieval foundry. Environmental microbiology 10 27943589
2009 Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects. Journal of human genetics 10 19343046
1987 Hemoglobin Grange-Blanche [beta 27(B9) Ala----Val], a new variant with normal expression and increased affinity for oxygen. FEBS letters 10 3666141
2025 The solute carrier superfamily interactome. Molecular systems biology 9 40355756
2021 Kinetics and molecular mechanism of enhanced fluoranthene biodegradation by co-substrate phenol in co-culture of Stenotrophomonas sp. N5 and Advenella sp. B9. Environmental research 9 34861230
2020 The Stimulation of Neurogenesis Improves the Cognitive Status of Aging Rats Subjected to Gestational and Perinatal Deficiency of B9-12 Vitamins. International journal of molecular sciences 9 33126444
2014 Homeobox B9 is overexpressed in hepatocellular carcinomas and promotes tumor cell proliferation both in vitro and in vivo. Biochemical and biophysical research communications 9 24462859
2006 Cytochrome P450 expression-induction profile and chemically mediated alterations of the WIF-B9 cell line. Biology of the cell 9 16354159
2006 Long-term prevention of diabetes and marked suppression of insulin autoantibodies and insulitis in mice lacking native insulin B9-23 sequence. Annals of the New York Academy of Sciences 9 17130542
2001 Posttranscriptional mechanisms of glucocorticoid antiproliferative effects: glucocorticoids inhibit IL-6-induced proliferation of B9 hybridoma cells. Cell transplantation 9 11332630
1988 Evidence for a projection from the B9 serotonergic cell group to the median raphe nucleus. Brain research bulletin 9 3191415
2022 Protocadherin B9 Is Associated with Human Esophageal Squamous Cell Carcinoma Progression. Pathobiology : journal of immunopathology, molecular and cellular biology 8 35390792
2022 First report on Vitamin B9 production including quantitative analysis of its vitamers in the yeast Scheffersomyces stipitis. Biotechnology for biofuels and bioproducts 8 36123695
2019 Complete genome sequence of Janthinobacterium sp. B9-8, a violacein-producing bacterium isolated from low-temperature sewage. Microbial pathogenesis 8 30610900
2018 Microbial Degradation of Amino Acid-Containing Compounds Using the Microcystin-Degrading Bacterial Strain B-9. Marine drugs 8 29415445
2018 Mechanisms involved in the death of steatotic WIF-B9 hepatocytes co-exposed to benzo[a]pyrene and ethanol: a possible key role for xenobiotic metabolism and nitric oxide. Free radical biology & medicine 8 30268890
2017 Homeobox B9 facilitates hypertrophic scar formation via activating the mitogen-activated protein kinase signaling pathway. Molecular medicine reports 8 28656236
2010 Ultra high throughput sequencing in human DNA variation detection: a comparative study on the NDUFA3-PRPF31 region. PloS one 8 20927379
2007 Effects of phenolic monomers on the enzymes activities and volatile fatty acids production of Neocallimastix frontalis B9. Anaerobe 8 18083606
2022 Functional characterization of a novel violacein biosynthesis operon from Janthinobacterium sp. B9-8. Applied microbiology and biotechnology 7 35445857
2014 The Medicago truncatula hypermycorrhizal B9 mutant displays an altered response to phosphate and is more susceptible to Aphanomyces euteiches. Plant, cell & environment 7 24815324
2013 Prevalence of metilentetrahidrofolate reductase C677T polymorphism, consumption of vitamins B6, B9, B12 and determination of lipidic hydroperoxides in obese and normal weight Mexican population. Nutricion hospitalaria 7 24506394
2012 Basic biology and mechanisms of neural ciliogenesis and the B9 family. Molecular neurobiology 7 22644387
1995 Lipopolysaccharide in concentrations above 40 ng/ml stimulates proliferation of the IL-6-dependent B9 cell line. Journal of immunological methods 7 7714331
1995 Isolation of an IL-13-dependent subclone of the B9 cell line useful for the estimation of human IL-13 bioactivity. Journal of immunological methods 7 7730664
1990 Structure and function of hemoglobin variants at an internal hydrophobic site: consequences of mutations at the beta 27 (B9) position. Biochemistry 7 2223757
2025 Dysfunctional one-carbon metabolism identifies vitamins B6, B9, B12, and choline as neuroprotective in glaucoma. Cell reports. Medicine 6 40345183
2023 E, K, B5, B6, and B9 vitamins and their specific immunological effects evaluated by flow cytometry. Frontiers in medicine 6 36687400
2022 Electrospinning of Microstructures Incorporated with Vitamin B9 for Food Application: Characteristics and Bioactivities. Polymers 6 36297915
2020 Involvement of supralemniscal nucleus (B9) 5-HT neuronal system in nociceptive processing: a fiber photometry study. Molecular brain 6 32005128
2019 Evaluation of oxidative stress mechanisms and the effects of phytotherapic extracts on Parkinson's disease Drosophila PINK1 model. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 6 31291788
2018 The imbalance of serotonergic circuitry impairing the crop supercontractile muscle activity and the mitochondrial morphology of PD PINK1B9Drosophila melanogaster are rescued by Mucuna pruriens. Journal of insect physiology 6 30393142
2015 Full genome sequence of Brevibacillus laterosporus strain B9, a biological control strain isolated from Zhejiang, China. Journal of biotechnology 6 26022423