Affinage

NDFIP1

NEDD4 family-interacting protein 1 · UniProt Q9BT67

Length
221 aa
Mass
24.9 kDa
Annotated
2026-06-10
49 papers in source corpus 22 papers cited in narrative 22 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NDFIP1 is a Golgi/endosomal membrane-anchored adaptor that activates HECT-type Nedd4-family E3 ubiquitin ligases, coupling them to substrates to control protein abundance and subcellular trafficking across diverse physiological settings (PMID:11748237, PMID:19706893). It binds Nedd4-family WW domains through two N-terminal PPxY motifs and is itself ubiquitinated; mechanistically it functions as a scaffold that bridges substrate to ligase and additionally recruits the E2 conjugating enzyme UbcH7 to Itch via its N-terminal region, thereby enhancing ligase activity (PMID:11748237, PMID:25632008). Through this adaptor activity NDFIP1 governs iron homeostasis at multiple nodes—directing Nedd4-2-mediated ubiquitination and degradation of the metal importer DMT1 to limit neuronal metal toxicity and to set systemic iron absorption, and enabling hepcidin-induced degradation of the iron exporter ferroportin (PMID:19706893, PMID:20959604, PMID:34320783). In the immune system it restrains inflammation by promoting Itch-dependent degradation of JunB and RORγT and Itch-mediated TAK1 ubiquitination in T cells, by limiting mTORC1 activity and glycolysis to sustain Treg Foxp3 expression, and by directing Nedd4-2-mediated ubiquitination of phosphorylated Syk to dampen mast cell FcεRI signaling (PMID:17137798, PMID:22080920, PMID:25632008, PMID:28580955, PMID:28051111, PMID:27786273). NDFIP1 also controls signaling protein localization, driving ubiquitination-dependent nuclear import of PTEN (in cooperation with Rab5) to regulate proliferation and ischemic neuronal survival, and of BRAT1 to maintain ATM activation after DNA damage (PMID:22213801, PMID:24798731, PMID:25801959, PMID:25631046). Beyond degradative ubiquitination, NDFIP1 loads Nedd4-family ligases and substrates such as TAZ into exosomes for intercellular removal (PMID:18819914, PMID:36929005), and it negatively regulates the antiviral adaptor MAVS, the TRESK potassium channel, neuronal dendritic development, and spatial memory (PMID:23087404, PMID:39201565, PMID:23897647, PMID:37023120).

Mechanistic history

Synthesis pass · year-by-year structured walk · 20 steps
  1. 2001 High

    Established NDFIP1 as a Golgi-localized adaptor that physically engages Nedd4-family ligases, defining its core molecular role in ubiquitin-dependent sorting.

    Evidence WW-domain binding/Co-IP, fractionation, and overexpression morphology in mammalian cells

    PMID:11748237

    Open questions at the time
    • Substrates engaged through this adaptor not yet identified
    • Physiological consequence beyond Golgi disruption unknown
  2. 2006 High

    Defined a physiological substrate axis by showing NDFIP1 enables Itch-mediated JunB degradation in T cells, linking the adaptor to immune homeostasis.

    Evidence Ndfip1 knockout mouse, Co-IP, and JunB half-life assays with T cell functional readouts

    PMID:17137798

    Open questions at the time
    • Whether NDFIP1 acts on other Itch substrates not addressed
    • Mechanism coupling T cell activation to Ndfip1 induction unclear
  3. 2008 Medium

    Revealed a non-degradative output: NDFIP1 routes Nedd4-family ligases into exosomes, suggesting cargo-mediated intercellular removal of ligases.

    Evidence Exosome fractionation from transfected cells and primary neurons with Western blot

    PMID:18819914

    Open questions at the time
    • Functional consequence of ligase removal in recipient cells not shown
    • Single lab, ubiquitination dependence not dissected
  4. 2009 High

    Identified DMT1 as a NDFIP1/Nedd4-2 substrate, establishing a metal-sensing adaptor function that protects neurons from iron/cobalt toxicity.

    Evidence Reciprocal Co-IP, shRNA/overexpression, ubiquitination assay, and Ndfip1-/- mouse brain analysis

    PMID:19706893

    Open questions at the time
    • How metal exposure triggers DMT1-NDFIP1 binding mechanistically unclear
    • Other metal transporters not tested
  5. 2010 Medium

    Extended NDFIP1 substrate scope to EGFR-pathway components (PTEN, c-Cbl, Src family) and connected it to Akt/Jnk signaling and proliferation control.

    Evidence siRNA depletion, Co-IP, ubiquitination assay, and EGF signaling/proliferation readouts in HeLa

    PMID:20534535

    Open questions at the time
    • Direct vs indirect effects on each substrate not fully separated
    • Single lab
  6. 2010 High

    Demonstrated NDFIP1 as a critical in vivo regulator of systemic iron via duodenal DMT1, separating iron from immune phenotypes.

    Evidence Ndfip1-/- and Ndfip1-/-/Rag1-/- mice, iron absorption and serum/tissue iron measurements

    PMID:20959604

    Open questions at the time
    • Ligase identity in enterocytes not confirmed in this study
    • Tissue-specific contributions not dissected
  7. 2012 High

    Showed NDFIP1 controls PTEN nuclear import through ubiquitination, defining a trafficking (rather than purely degradative) function with consequences for ischemic neuronal survival.

    Evidence Ndfip1-/- ischemia model, Co-IP, ubiquitination assay, FRAP live imaging, infarct measurement

    PMID:22213801

    Open questions at the time
    • Nuclear import machinery downstream of ubiquitination unresolved
    • Which Nedd4 ligase predominates not pinned down
  8. 2012 Medium

    Broadened NDFIP1's regulatory range to innate antiviral signaling by linking it to Smurf1-mediated MAVS degradation.

    Evidence Overexpression/siRNA, Co-IP, luciferase reporters, IRF-3 phosphorylation and ubiquitination assays

    PMID:23087404

    Open questions at the time
    • In vivo viral infection phenotype not shown
    • Single lab; Smurf1 vs other ligase specificity unclear
  9. 2012 Medium

    Confirmed endogenous NDFIP1-Nedd4-2 association in brain and distinguished an Ndfip1-independent Itch survival pathway during ischemia.

    Evidence Endogenous Co-IP from brain tissue, immunohistochemistry, rat ischemia model

    PMID:22417925

    Open questions at the time
    • Functional consequence of the brain Nedd4-2 interaction not defined
    • Single lab
  10. 2013 Medium

    Established a developmental role: NDFIP1 is required for normal cortical dendrite and spine maturation linked to PI3K/Akt signaling.

    Evidence Neuron-specific conditional KO, EM, dendritic morphology, PSD fractionation, Western blot

    PMID:23897647

    Open questions at the time
    • Specific substrate driving the dendritic phenotype not identified
    • Single lab
  11. 2014 Medium

    Placed PTEN ubiquitination/nuclear trafficking on the endosomal Rab5 pathway, integrating NDFIP1 with vesicular trafficking machinery.

    Evidence BiFC imaging, dominant-negative Rab5, Ndfip1 deletion, Co-IP, ubiquitination assay

    PMID:24798731

    Open questions at the time
    • How Rab5 endosomes deliver PTEN to the nucleus mechanistically unclear
    • Single lab
  12. 2015 High

    Resolved the biochemical basis of ligase activation by mapping NDFIP1 as an E2 (UbcH7)-recruiting scaffold for Itch, with functional impact on airway inflammation.

    Evidence Co-IP, Ndfip1 domain-mutant rescue, ubiquitination assay, Ndfip1-/- and Itch-/- airway models

    PMID:25632008

    Open questions at the time
    • Whether UbcH7 recruitment generalizes to other Nedd4-family ligases not tested
    • TAK1 ubiquitin linkage type not fully characterized
  13. 2015 Medium

    Showed NDFIP1 directs BRAT1 nuclear translocation to sustain ATM activation, extending its trafficking function to the DNA damage response.

    Evidence Co-IP, knockdown/overexpression, nuclear fractionation, genotoxic stress and brain injury models

    PMID:25631046

    Open questions at the time
    • Ubiquitin linkage and ligase identity for BRAT1 not defined
    • Single lab
  14. 2015 Medium

    Demonstrated NDFIP1 sets nuclear PTEN levels to control cell-cycle regulators and proliferation, with dosage effects on brain size.

    Evidence Ndfip1 KO and brain transgenic overexpression, fractionation, proliferation assays, cyclin D1/Plk1 blots

    PMID:25801959

    Open questions at the time
    • Mechanism linking nuclear PTEN to Plk1/cyclin D1 not detailed
    • Single lab
  15. 2016 High

    Identified phospho-Syk as a Nedd4-2/NDFIP1 substrate that dampens mast cell FcεRI signaling, defining an allergic-response regulatory node.

    Evidence Nedd4-2 and Ndfip1 KO mast cells, Syk ubiquitination assay, in vivo cutaneous anaphylaxis

    PMID:27786273

    Open questions at the time
    • Whether NDFIP1 acts on other signalosome components unclear
  16. 2017 High

    Showed NDFIP1 restrains Treg mTORC1 and glycolysis via a mechanism distinct from its IL-4 control, linking the adaptor to immunometabolism and Foxp3 stability.

    Evidence Treg-specific conditional KO, Seahorse metabolic profiling, proteomics, mTORC1 assays, lineage tracing

    PMID:28580955

    Open questions at the time
    • Substrate mediating mTORC1 restraint not identified
    • Single lab
  17. 2017 Medium

    Added RORγT as a NDFIP1-controlled substrate limiting Th17 expansion and colitogenic potential.

    Evidence Ndfip1-/- mouse, Th17 differentiation, RORγT level analysis, transfer colitis model

    PMID:28051111

    Open questions at the time
    • Direct ubiquitination of RORγT not demonstrated
    • Ligase identity not defined
  18. 2023 Medium

    Revealed a degradation-independent output: NDFIP1 packages TAZ into exosomes via WW-domain recognition to limit TAZ accumulation and tumor cell proliferation.

    Evidence Co-IP, exosome isolation, NDFIP1 KO, proliferation assays, xenograft model

    PMID:36929005

    Open questions at the time
    • Whether ubiquitination is required for TAZ exosomal loading unclear
    • Single lab
  19. 2023 Medium

    Defined NDFIP1 as a negative regulator of spatial memory through Nedd4-1-dependent ubiquitination of Beclin 1 and PTEN in hippocampus.

    Evidence Endogenous Co-IP, hippocampal ubiquitination assays, conditional het/KO mice, water maze

    PMID:37023120

    Open questions at the time
    • Causal substrate driving memory phenotype not isolated
    • Single lab
  20. 2024 Medium

    Extended NDFIP1's targets to ion channels, showing PPxY/Nedd4-dependent ubiquitination and suppression of the TRESK potassium current.

    Evidence Xenopus oocyte voltage clamp, Co-IP, PPxY mutagenesis, dominant-negative Nedd4, ubiquitination assay

    PMID:39201565

    Open questions at the time
    • Physiological relevance in native neurons not shown
    • Single lab; heterologous system

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NDFIP1 selects among its many Nedd4-family ligases and dictates whether a substrate is degraded, trafficked to the nucleus, or exported via exosomes remains unresolved.
  • No unifying model for substrate-versus-fate specificity
  • No structural data on the E2-adaptor-E3 ternary complex
  • Determinants routing cargo to exosomes vs proteasome unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 3 GO:0140096 catalytic activity, acting on a protein 3 GO:0098772 molecular function regulator activity 2
Localization
GO:0031410 cytoplasmic vesicle 2 GO:0005768 endosome 1 GO:0005794 Golgi apparatus 1
Pathway
R-HSA-168256 Immune System 5 GO:0140096 catalytic activity, acting on a protein 3 R-HSA-382551 Transport of small molecules 3 R-HSA-392499 Metabolism of proteins 3 R-HSA-9609507 Protein localization 3
Partners
DMT1ITCHNEDD4NEDD4LPTENSMURF1UBE2L3WWTR1

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 NDFIP1 (N4WBP5) binds Nedd4 family WW domains via two PPXY motifs in its amino terminus, is itself ubiquitinated, localizes to the Golgi complex, and its ectopic expression disrupts Golgi structure, suggesting a role as an adaptor for Nedd4-like proteins in ubiquitin-dependent protein sorting. Co-immunoprecipitation/WW-domain binding assays, subcellular fractionation, immunofluorescence, overexpression morphological analysis The Journal of biological chemistry High 11748237
2006 Ndfip1 promotes the function of the E3 ubiquitin ligase Itch to ubiquitinate and reduce the half-life of JunB in T cells; absence of Ndfip1 leads to JunB accumulation, Th2 cytokine production, and inflammatory disease. T cell activation promotes Ndfip1 expression and its physical association with Itch. Ndfip1 knockout mouse, co-immunoprecipitation, JunB half-life measurement, T cell functional assays Immunity High 17137798
2008 Ndfip1 is sorted into exosomes and increases exosome secretion from cells; Ndfip1 expression recruits Nedd4, Nedd4-2, and Itch (which are otherwise absent) into exosomes, providing a mechanism for cargo-mediated removal of Nedd4 family proteins via the multivesicular body/exosome pathway. Exosome isolation and characterization, transfection overexpression, primary neuron exosome analysis, Western blot The Journal of biological chemistry Medium 18819914
2009 Ndfip1 acts as an adaptor that binds DMT1 in response to iron or cobalt exposure in human neurons, recruits the E3 ligase Nedd4-2 to ubiquitinate DMT1, leading to DMT1 degradation, reduced metal entry, and protection from metal toxicity. Ndfip1 knockout mice accumulate iron in neurons. Co-immunoprecipitation, shRNAi knockdown, overexpression, ubiquitination assay, metal toxicity assay, Ndfip1−/− mouse brain analysis Proceedings of the National Academy of Sciences of the United States of America High 19706893
2010 Ndfip1 and Ndfip2 associate with the EGF receptor and PTEN and control ubiquitination and protein abundance of PTEN, c-Cbl, and Src family kinases; depletion of Ndfip1 inhibits Akt activation in EGF-stimulated HeLa cells, stimulates Jnk activation, and enhances cell multiplication. Ndfip2 (but not Ndfip1) is phosphorylated by Src/Lyn and can scaffold Src phosphorylation of Ndfip1. siRNA depletion, co-immunoprecipitation, ubiquitination assay, EGF signaling pathway analysis, cell proliferation assay Proceedings of the National Academy of Sciences of the United States of America Medium 20534535
2010 Ndfip1 knockout mice fed a low-iron diet show significantly higher DMT1 expression and activity in duodenal enterocytes, elevated serum iron and transferrin saturation, and increased liver/spleen iron stores, demonstrating that Ndfip1 is a critical in vivo regulator of DMT1 and systemic iron homeostasis. Ndfip1−/− mouse model, intestinal iron absorption assay, serum iron measurement, tissue iron quantification, Ndfip1−/−/Rag1−/− immunodeficient mice Blood High 20959604
2011 TGF-β signaling transiently induces Ndfip1 expression during iTreg differentiation; once expressed, Ndfip1 promotes Itch-mediated ubiquitination and degradation of JunB, preventing IL-4 production and permitting sustained Foxp3 expression and iTreg cell differentiation. Ndfip1−/− T cell in vitro differentiation assays, cytokine measurement, JunB protein level analysis, TGF-β stimulation experiments Nature immunology High 22080920
2012 Ndfip1 is required for the translocation of cytoplasmic Pten into neuronal nuclei after cerebral ischemia; Ndfip1 binds Pten, enhances its ubiquitination by Nedd4 E3 ligases, and increases the rate of Pten nuclear import as measured by FRAP. Ndfip1-deficient neurons fail to import Pten and suffer larger infarcts with suppressed pAkt activation. Ndfip1−/− mouse ischemia model, co-immunoprecipitation, ubiquitination assay, FRAP live imaging, infarct size measurement, pAkt Western blot The Journal of cell biology High 22213801
2012 Ndfip1 negatively regulates RIG-I-dependent antiviral signaling by enhancing Smurf1 self-ubiquitination and facilitating Smurf1 interaction with MAVS, promoting K48-linked ubiquitination and proteasomal degradation of MAVS. Ndfip1 knockdown elevates MAVS levels and amplifies IFN-β and NF-κB signaling. Overexpression, siRNA knockdown, co-immunoprecipitation, luciferase reporter assays, IRF-3 phosphorylation assay, ubiquitination assay Journal of immunology Medium 23087404
2012 Endogenous Ndfip1 binds Nedd4-2 in the brain (with or without ischemia), as confirmed by immunoprecipitation. Ischemia-induced Itch upregulation promotes neuronal survival independently of Ndfip1. Co-immunoprecipitation from brain tissue, immunohistochemistry, ischemia model in rats Experimental neurology Medium 22417925
2014 Rab5 GTPase cooperates with Ndfip1 in Pten ubiquitination and nuclear trafficking; Rab5 colocalizes with Pten on endosomes, dominant-negative Rab5 reduces Pten ubiquitination and nuclear import, and Ndfip1 deletion abrogates nuclear trafficking of ubiquitinated Pten even in the presence of Rab5. Bimolecular fluorescence complementation (BiFC) for protein interaction imaging, dominant-negative Rab5 expression, Ndfip1 genomic deletion, co-immunoprecipitation, ubiquitination assay Traffic Medium 24798731
2015 Ndfip1 mediates ubiquitination of BRAT1 (a BRCA1-associated ATM activator), which is required for BRAT1 nuclear translocation during the DNA damage response. Without Ndfip1, BRAT1 fails to enter the nucleus and ATM phosphorylation is not maintained. Genotoxic stress increases both Ndfip1 and phospho-ATM levels. Co-immunoprecipitation, overexpression/knockdown, nuclear fractionation, genotoxic stress assays, brain injury model immunostaining The Journal of biological chemistry Medium 25631046
2015 Ndfip1 controls Pten nuclear compartmentalization during the cell cycle; Ndfip1 deletion abolishes nuclear Pten without affecting cytoplasmic Pten-PI3K/Akt activity, leading to dysregulated Plk1 and cyclin D1 levels and increased cell proliferation. Transgenic Ndfip1 overexpression in the developing brain increases nuclear Pten, lengthens neuronal progenitor cell cycle, and causes microencephaly. Ndfip1 genetic deletion, subcellular fractionation, cell proliferation assays, brain transgenic overexpression, cyclin D1/Plk1 Western blot Journal of molecular cell biology Medium 25801959
2015 Ndfip1 enhances Itch E3 ligase activity by recruiting the E2 ubiquitin-conjugating enzyme UbcH7 to Itch; the N-terminal region of Ndfip1 binds UbcH7 while its PY motif binds Itch, making Ndfip1 a scaffold/adaptor bridging E2 and E3. Ndfip1 facilitates Itch-mediated TAK1 ubiquitination and restrains proinflammatory cytokine production in airway inflammation. Co-immunoprecipitation, Ndfip1 mutant rescue experiments, ubiquitination assay, Ndfip1−/− and Itch−/− mouse airway inflammation model Journal of immunology High 25632008
2016 Nedd4-2 and Ndfip1 limit IgE/FcεRI-mediated mast cell activation by ubiquitinating phosphorylated Syk tyrosine kinase, thereby attenuating downstream FcεRI signalosome activity. Loss of Nedd4-2 or Ndfip1 in mast cells results in exacerbated and prolonged IgE-mediated cutaneous anaphylaxis in vivo. Nedd4-2 and Ndfip1 knockout mast cells, ubiquitination assay for Syk, in vivo cutaneous anaphylaxis model Nature communications High 27786273
2017 Ndfip1 restricts mTORC1 signaling and glycolysis in Treg cells; Ndfip1-deficient Tregs show elevated mTORC1 activity and glycolysis. Ndfip1 restricts IL-4 production and mTORC1 signaling via distinct mechanisms (IL-4 deficiency does not prevent mTORC1 elevation). Ndfip1 deletion in Tregs causes autoinflammatory disease with loss of Foxp3 expression. Treg-specific Ndfip1 conditional knockout, metabolic profiling (Seahorse), proteomics, mTORC1 signaling assays, Foxp3 lineage tracing Nature communications High 28580955
2017 Ndfip1 promotes degradation of RORγT in Th17 cells; Ndfip1-deficient Th17 cells accumulate RORγT and have increased frequency, enhanced IL-17 production, and greater colitis-inducing capacity in vivo. Ndfip1−/− mouse, Th17 cell differentiation assays, RORγT protein level analysis, in vivo transfer colitis model Scientific reports Medium 28051111
2022 NDFIP1 is required for hepcidin-induced ubiquitination and degradation of ferroportin (the iron export channel); siRNA depletion of NDFIP1 prevents BMP6- and exogenous hepcidin-induced ferroportin degradation in HepG2 cells, and AAV-mediated silencing of Ndfip1 in mouse liver increases hepatic ferroportin and circulating iron. siRNA screen of 88 ubiquitin pathway components, FPN-GFP inducible reporter cell line, AAV-mediated in vivo silencing, serum iron measurement Haematologica High 34320783
2023 NDFIP1 limits cellular TAZ (WWTR1) accumulation by packaging it into exosomes; NDFIP1 interacts with TAZ via WW-domain recognition, and NDFIP1 knockout leads to TAZ accumulation without changes in mRNA or degradation rate. NDFIP1-mediated TAZ exosomal export suppresses NSCLC cell proliferation in vitro and in vivo. Co-immunoprecipitation, exosome isolation, NDFIP1 knockout, cell proliferation assays, xenograft mouse model Protein & cell Medium 36929005
2024 NDFIP1 directly interacts with the TRESK (KCNK18) background potassium channel and negatively regulates its current; coexpression of NDFIP1 abolishes TRESK current in Xenopus oocytes, dependent on intact PPxY motifs (required for Nedd4 interaction) and partially reversed by dominant-negative Nedd4. NDFIP1 coexpression induces ubiquitination of TRESK. Xenopus oocyte two-electrode voltage clamp, co-immunoprecipitation, PPxY motif mutagenesis, dominant-negative Nedd4 overexpression, ubiquitination assay International journal of molecular sciences Medium 39201565
2013 Ndfip1 is required for the normal development of pyramidal neuron dendrites and dendritic spines in the neocortex; conditional knockout neurons show stunted dendritic arbors, fewer spines, reduced postsynaptic density proteins (Arc, PSD-95), and altered PI3K/Akt signaling. Conditional neuron-specific Ndfip1 knockout mouse, electron microscopy, dendritic morphology analysis, PSD fractionation, Western blot for signaling components Cerebral cortex Medium 23897647
2023 Ndfip1 acts as a negative regulator of spatial memory formation; spatial training decreases Ndfip1 expression and reduces its association with Nedd4-1, leading to decreased ubiquitination of Beclin 1 and PTEN in the hippocampus and increased levels of both proteins. Ndfip1 conditional heterozygous mice show enhanced spatial memory. Co-immunoprecipitation, ubiquitination assay in hippocampal tissue, conditional heterozygous and KO mice, water maze behavioral testing PloS one Medium 37023120

Source papers

Stage 0 corpus · 49 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2008 Nedd4 family-interacting protein 1 (Ndfip1) is required for the exosomal secretion of Nedd4 family proteins. The Journal of biological chemistry 136 18819914
2006 Ndfip1 protein promotes the function of itch ubiquitin ligase to prevent T cell activation and T helper 2 cell-mediated inflammation. Immunity 124 17137798
2012 Ndfip1 negatively regulates RIG-I-dependent immune signaling by enhancing E3 ligase Smurf1-mediated MAVS degradation. Journal of immunology (Baltimore, Md. : 1950) 112 23087404
2001 N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein. The Journal of biological chemistry 109 11748237
2009 Divalent metal transporter 1 (DMT1) regulation by Ndfip1 prevents metal toxicity in human neurons. Proceedings of the National Academy of Sciences of the United States of America 104 19706893
2012 Ndfip1 regulates nuclear Pten import in vivo to promote neuronal survival following cerebral ischemia. The Journal of cell biology 92 22213801
2010 Regulation of PTEN/Akt and MAP kinase signaling pathways by the ubiquitin ligase activators Ndfip1 and Ndfip2. Proceedings of the National Academy of Sciences of the United States of America 68 20534535
2011 TGF-β induces the expression of the adaptor Ndfip1 to silence IL-4 production during iTreg cell differentiation. Nature immunology 61 22080920
2006 Nedd4-WW domain-binding protein 5 (Ndfip1) is associated with neuronal survival after acute cortical brain injury. The Journal of neuroscience : the official journal of the Society for Neuroscience 48 16822981
2017 MiR-155 Promotes Uveal Melanoma Cell Proliferation and Invasion by Regulating NDFIP1 Expression. Technology in cancer research & treatment 44 29333944
2010 Ndfip1-deficient mice have impaired DMT1 regulation and iron homeostasis. Blood 40 20959604
2017 Ndfip1 restricts mTORC1 signalling and glycolysis in regulatory T cells to prevent autoinflammatory disease. Nature communications 35 28580955
2016 The Nedd4-2/Ndfip1 axis is a negative regulator of IgE-mediated mast cell activation. Nature communications 35 27786273
2019 The miR-873/NDFIP1 axis promotes hepatocellular carcinoma growth and metastasis through the AKT/mTOR-mediated Warburg effect. American journal of cancer research 33 31218102
2014 Ndfip1 attenuated 6-OHDA-induced iron accumulation via regulating the degradation of DMT1. Neurobiology of aging 32 25467637
2012 Differential regulation of Nedd4 ubiquitin ligases and their adaptor protein Ndfip1 in a rat model of ischemic stroke. Experimental neurology 32 22417925
2015 Nedd4 family interacting protein 1 (Ndfip1) is required for ubiquitination and nuclear trafficking of BRCA1-associated ATM activator 1 (BRAT1) during the DNA damage response. The Journal of biological chemistry 28 25631046
2010 The ubiquitin ligase adaptor Ndfip1 regulates T cell-mediated gastrointestinal inflammation and inflammatory bowel disease susceptibility. Mucosal immunology 28 20962770
2015 Ndfip1 represses cell proliferation by controlling Pten localization and signaling specificity. Journal of molecular cell biology 27 25801959
2014 Increased Ndfip1 in the substantia nigra of Parkinsonian brains is associated with elevated iron levels. PloS one 27 24475238
2014 Ndfip1 mediates peripheral tolerance to self and exogenous antigen by inducing cell cycle exit in responding CD4+ T cells. Proceedings of the National Academy of Sciences of the United States of America 24 24520172
2022 UBA6 and NDFIP1 regulate the degradation of ferroportin. Haematologica 23 34320783
2014 Rab5 and Ndfip1 are involved in Pten ubiquitination and nuclear trafficking. Traffic (Copenhagen, Denmark) 22 24798731
2018 Lower Expression of Ndfip1 Is Associated With Alzheimer Disease Pathogenesis Through Decreasing DMT1 Degradation and Increasing Iron Influx. Frontiers in aging neuroscience 21 29937728
2024 Ndfip1 protected dopaminergic neurons via regulating mitochondrial function and ferroptosis in Parkinson's disease. Experimental neurology 17 38365133
2017 Ndfip1 restricts Th17 cell potency by limiting lineage stability and proinflammatory cytokine production. Scientific reports 16 28051111
2020 Nicotine promotes tumor progression and epithelial-mesenchymal transition by regulating the miR-155-5p/NDFIP1 axis in pancreatic ductal adenocarcinoma. Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 15 32354626
2019 chi-miR-143-3p Promotes Apoptosis of Mammary Gland Epithelial Cells from Dairy Goats by Targeting Ndfip1. DNA and cell biology 14 31603699
2015 Ndfip1 regulates itch ligase activity and airway inflammation via UbcH7. Journal of immunology (Baltimore, Md. : 1950) 13 25632008
2023 MiR-155-5p Aggravated Astrocyte Activation and Glial Scarring in a Spinal Cord Injury Model by Inhibiting Ndfip1 Expression and PTEN Nuclear Translocation. Neurochemical research 12 36750528
2013 Ndfip1 enforces a requirement for CD28 costimulation by limiting IL-2 production. Journal of immunology (Baltimore, Md. : 1950) 12 23851689
2010 Cellular up-regulation of Nedd4 family interacting protein 1 (Ndfip1) using low levels of bioactive cobalt complexes. The Journal of biological chemistry 12 21187286
2021 Ndfip1 Prevents Rotenone-Induced Neurotoxicity and Upregulation of α-Synuclein in SH-SY5Y Cells. Frontiers in molecular neuroscience 11 33469419
2018 The Ubiquitin Ligase Adaptor NDFIP1 Selectively Enforces a CD8+ T Cell Tolerance Checkpoint to High-Dose Antigen. Cell reports 11 30021156
2013 Ndfip1 is required for the development of pyramidal neuron dendrites and spines in the neocortex. Cerebral cortex (New York, N.Y. : 1991) 11 23897647
2023 NDFIP1 limits cellular TAZ accumulation via exosomal sorting to inhibit NSCLC proliferation. Protein & cell 9 36929005
2013 Ndfip1 expression in developing neurons indicates a role for protein ubiquitination by Nedd4 E3 ligases during cortical development. Neuroscience letters 7 24036464
2023 Research progress on the role of Ndfip1 (Nedd4 family interacting protein 1) in immune cells. Allergologia et immunopathologia 6 36617825
2015 Protective effects of Ndfip1 on MPP(+)-induced apoptosis in MES23.5 cells and its underlying mechanisms. Experimental neurology 6 26300475
2015 Nedd4 family interacting protein 1 (Ndfip1) promotes death of pancreatic beta cells. Biochemical and biophysical research communications 5 26319551
2012 Gastrointestinal microbiota do not significantly contribute to T cell activation or GI inflammation in Ndfip1-cKO mice. PloS one 5 22506022
2022 Tyrphostin AG1024 Suppresses Coronaviral Replication by Downregulating JAK1 via an IR/IGF-1R Independent Proteolysis Mediated by Ndfip1/2_NEDD4-like E3 Ligase Itch. Pharmaceuticals (Basel, Switzerland) 4 35215353
2013 Iron status and lipopolysaccharide regulate Ndfip1 by activation of nuclear factor-kappa B. Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine 4 24045929
2023 Identification of Ndfip1 as a novel negative regulator for spatial memory formation associated with increased ubiquitination of Beclin 1 and PTEN. PloS one 3 37023120
2021 Genetic variation in NDFIP1 modifies the metabolic patterns in immune cells of multiple sclerosis patients. Scientific reports 3 34725369
2024 The Ubiquitin Ligase Adaptor NDFIP1 Interacts with TRESK and Negatively Regulates the Background K+ Current. International journal of molecular sciences 2 39201565
2025 Differentially Expressed Nedd4-binding Protein Ndfip1 Protects Neurons Against Methamphetamine-induced Neurotoxicity. Neurotoxicity research 1 39808388
2025 Cabergoline-induced NDFIP1 upregulation in pituitary neuroendocrine tumor cells activates mTOR signaling and contributes to cabergoline resistance. Journal of neuro-oncology 1 39891847
2025 Nedd4 family interacting protein 1 (NDFIP1) is a novel target of PF-429242 in promoting autophagy in hepatocellular carcinoma cells. Biochemical and biophysical research communications 0 41317707

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