Affinage

NABP2

SOSS complex subunit B1 · UniProt Q9BQ15

Length
211 aa
Mass
22.3 kDa
Annotated
2026-06-10
61 papers in source corpus 20 papers cited in narrative 20 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 5/5 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NABP2 (hSSB1/OBFC2B) is a single-stranded DNA-binding protein that acts as an apical regulator of the genome stability response, operating upstream of the MRN complex and ATM at DNA double-strand breaks (DSBs) (PMID:18449195, PMID:21051358). It is rapidly recruited to break sites independently of CtIP, MDC1, and MRN, and its loss abrogates ATM activation, MRN focus formation, and resection-dependent ssDNA/RPA generation, placing it at the head of the DSB signaling cascade (PMID:18449195, PMID:21051358). Mechanistically, hSSB1 directly binds NBS1 and stimulates MRN endonuclease activity through its C-terminal tail to drive resection and subsequent RAD51/BRCA1 loading for homologous recombination (PMID:21227926, PMID:23986477). Beyond DSBs, hSSB1 acts at hydroxyurea-stalled replication forks to enable ATR/Chk1 activation and fork restart (PMID:24753408), and participates in base excision repair by complexing with hOGG1 and binding 8-oxoguanine-containing DNA, a BER role that uniquely requires its redox-dependent oligomerization via C81/C99 whereas HR does not (PMID:26261212, PMID:27273218, PMID:28609781). hSSB1 stability is heavily regulated by post-translational modification: ATM phosphorylation at T117 counteracts FBXL5-SCF-mediated ubiquitin-proteasome degradation (PMID:18449195, PMID:25249620), DNA-PK phosphorylates S134 to promote survival after fork disruption (PMID:28448822), and PIAS2α-catalyzed SUMOylation at K79/K94 stabilizes the protein and promotes NBS1 recruitment (PMID:32576812). It functions as a stable subunit of the SOSS1 complex with INTS3, C9orf80, and INTS6, which is required for RAD51/BRCA1 recruitment and HR (PMID:19605351, PMID:19786574, PMID:23986477). hSSB1 also stabilizes BLM helicase (PMID:28506294), binds G-strand telomeric DNA and recruits TERT to maintain G-overhangs (PMID:25589350), and modulates checkpoint responses by protecting p53 and p21 from degradation and promoting p300-mediated p53 acetylation (PMID:21242961, PMID:23184057).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 2008 High

    Established hSSB1 as an essential, ATM-regulated component of the DSB response, resolving whether a human single-OB-domain SSB participates in damage signaling.

    Evidence Cell-based phosphorylation assays, siRNA depletion, DSB foci, and ATM kinase readouts after ionizing radiation

    PMID:18449195

    Open questions at the time
    • Did not define the upstream recruitment determinants
    • Did not resolve how T117 phosphorylation stabilizes the protein
  2. 2009 High

    Defined hSSB1 as a subunit of a stable heterotrimeric complex (with INTS3 and C9orf80/MISE), explaining how its stability and HR function are scaffolded.

    Evidence Co-IP, tandem affinity purification with mass spectrometry, HR reporter and ATM signaling assays

    PMID:19605351 PMID:19786574

    Open questions at the time
    • Stoichiometry and structural architecture of the complex unresolved
    • Whether INTS3 acts only transcriptionally vs. post-translationally was ambiguous
  3. 2010 High

    Placed hSSB1 epistatically upstream of MRN, showing it is recruited independently of CtIP/MDC1/MRN yet is required for MRN recruitment and resection.

    Evidence Sequential siRNA depletion, laser microirradiation, BrdU ssDNA and RPA foci assays

    PMID:21051358

    Open questions at the time
    • The direct molecular trigger for hSSB1 recruitment to breaks not identified
  4. 2011 High

    Provided the biochemical mechanism for the hSSB1-MRN axis: direct NBS1 binding and C-terminal-tail-dependent stimulation of MRN endonuclease activity.

    Evidence In vitro reconstitution with purified proteins, pulldowns, C-terminal deletion and NBS1 disease mutants

    PMID:21227926

    Open questions at the time
    • Single lab reconstitution
    • Structural basis of NBS1 contact not solved
  5. 2011 Medium

    Linked hSSB1 to checkpoint control by showing it stabilizes p21 against degradation, partly explaining checkpoint defects on depletion.

    Evidence Co-IP, siRNA knockdown, ubiquitination and cell cycle analysis

    PMID:21242961

    Open questions at the time
    • Single lab
    • Direct vs. indirect protection of p21 not fully distinguished
  6. 2012 Medium

    Extended hSSB1's checkpoint role to the p53 axis, protecting p53 and promoting p300-mediated K382 acetylation to activate p21 transcription.

    Evidence Co-IP, ubiquitination/acetylation assays, luciferase reporter, cell cycle analysis

    PMID:23184057

    Open questions at the time
    • Single lab
    • Whether p53/p300 effects are separable from direct repair functions unclear
  7. 2014 High

    Demonstrated a distinct role at stalled replication forks, where hSSB1 enables ATR/Chk1 activation and Mre11/Rad51 recruitment for fork restart.

    Evidence siRNA depletion, HU/CPT treatment, DNA fiber assay, checkpoint kinase and foci assays

    PMID:24753408

    Open questions at the time
    • Molecular distinction between DSB and fork functions not defined here
  8. 2014 High

    Identified the degradation machinery (FBXL5-SCF) controlling hSSB1 abundance and the protective role of T117 phosphorylation, defining its stability switch.

    Evidence Co-IP, ubiquitination assays, proteasome inhibition, ATM kinase assay

    PMID:25249620

    Open questions at the time
    • How phosphorylation sterically/biochemically blocks FBXL5 binding not resolved
  9. 2015 High

    Established a base excision repair function via complex formation with hOGG1 and direct binding to 8-oxoguanine DNA, broadening hSSB1 beyond DSB/fork repair.

    Evidence Co-IP, chromatin fractionation, EMSA and in vitro OGG1 activity assays

    PMID:26261212

    Open questions at the time
    • Single lab
    • In vivo contribution of BER role to oxidative damage survival not quantified
  10. 2015 Medium

    Revealed a telomeric function: G-strand DNA binding and TERT recruitment required to maintain G-overhangs.

    Evidence EMSA with telomeric oligos, Co-IP with TERT, telomere ChIP and G-overhang assays

    PMID:25589350

    Open questions at the time
    • Single lab
    • Relationship between telomeric and DSB roles unclear
  11. 2015 Medium

    Showed an RPA-independent ATR activation route, with hSSB1/2-INTS3 recruiting ATR-ATRIP via TopBP1 and the 9-1-1 complex under genomic stress.

    Evidence Sequential siRNA depletion, ATRIP/ATR foci, Co-IP, Chk1 phosphorylation

    PMID:25916848

    Open questions at the time
    • Single lab
    • Physiological conditions favoring this alternate route not defined
  12. 2015 Medium

    Mapped the C-terminal tail as essential for DNA binding and protein stability and characterized the monomeric solution state with a disordered C-terminus.

    Evidence EMSA, ITC, CD spectroscopy, sucrose gradient, C-terminal deletion mutants

    PMID:26550690

    Open questions at the time
    • Single lab
    • Functional state in cells vs. in vitro not reconciled
  13. 2016 Medium

    Separated repair pathway requirements at the level of oligomeric state: redox-driven oligomerization is needed for BER but dispensable for HR.

    Evidence Size exclusion chromatography, EMSA with oxidized DNA, siRNA rescue with oligomerization mutants, 8-oxoG and HR assays

    PMID:27273218

    Open questions at the time
    • Single lab
    • In vivo trigger of oxidative oligomerization not established
  14. 2016 High

    Defined the structural ssDNA recognition mode in solution via base-stacking of four OB-domain aromatic residues, distinct from the crystallographic SOSS1 mode.

    Evidence Solution-state NMR chemical shift mapping, ITC, fluorescence and DNA binding assays

    PMID:27387285

    Open questions at the time
    • Single lab
    • Functional significance of the solution/crystal discrepancy not resolved
  15. 2017 High

    Confirmed an SSB-like tetramer stabilized by C81/C99 that retains INTS3 binding, integrating oligomerization with SOSS1 assembly; further defined the core complex including INTS6.

    Evidence Solution NMR, SEC, C81/C99 mutagenesis, INTS3/INTS6 Co-IP and in vitro reconstitution, RAD51/BRCA1 foci and HR assays

    PMID:23986477 PMID:28609781

    Open questions at the time
    • Single lab structural model
    • Cellular conditions selecting tetramer vs. monomer not defined
  16. 2017 High

    Added a second regulatory kinase, DNA-PK, phosphorylating S134 to promote survival after fork disruption, with PPP-family phosphatases setting the baseline.

    Evidence In vitro DNA-PK kinase assay, S134A mutagenesis, phosphatase depletion, survival assays

    PMID:28448822

    Open questions at the time
    • Single lab
    • Downstream effector of S134 phosphorylation not identified
  17. 2017 Medium

    Identified hSSB1 as a stabilizer of BLM helicase, required for BLM chromatin recruitment after DSBs and fork stalling.

    Evidence Co-IP, siRNA depletion, proteasome inhibition, chromatin fractionation, BLM foci

    PMID:28506294

    Open questions at the time
    • Single lab
    • Whether stabilization is via direct binding or indirect not fully resolved
  18. 2020 High

    Completed the post-translational regulatory map by defining SUMOylation at K79/K94 (writer PIAS2α, eraser SENP2) that stabilizes hSSB1 and promotes NBS1 recruitment.

    Evidence In vivo SUMOylation assays, K79R/K94R mutagenesis, PIAS2α/SENP2 Co-IP, NBS1 foci, IR survival, UBC9 knockout

    PMID:32576812

    Open questions at the time
    • Crosstalk among T117 phosphorylation, S134 phosphorylation, and SUMOylation not integrated
    • Whether SUMOylation acts on chromatin-bound vs. soluble pools unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • How hSSB1 is initially targeted to DSBs and stalled forks, and how its many modifications and oligomeric states are coordinated across HR, BER, replication, and telomere maintenance, remains unresolved.
  • Direct recruitment mechanism to damage sites not identified
  • Integrated model linking phospho/SUMO regulation to pathway choice missing
  • In vivo significance of telomeric and p53/p21 roles relative to core HR function not quantified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 5 GO:0060090 molecular adaptor activity 2 GO:0098772 molecular function regulator activity 2
Localization
GO:0000228 nuclear chromosome 2 GO:0005634 nucleus 1
Pathway
R-HSA-73894 DNA Repair 4 R-HSA-8953897 Cellular responses to stimuli 3 R-HSA-1640170 Cell Cycle 2 R-HSA-69306 DNA Replication 1
Partners
BLMC9ORF80FBXL5HOGG1INTS3INTS6NBS1TERT
Complex memberships
SOSS1 complex (INTS3-hSSB1-C9orf80/INTS6)

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2008 hSSB1 (NABP2) is phosphorylated by ATM kinase at threonine 117 in response to DNA double-strand breaks; this phosphorylation is required for DNA damage-induced stabilization of hSSB1. hSSB1 accumulates in the nucleus and forms distinct foci at DSB sites. Depletion of hSSB1 abrogates ATM activation and phosphorylation of ATM targets after ionizing radiation, establishing hSSB1 as required for ATM-mediated DSB signaling. Cell-based phosphorylation assays, siRNA depletion, nuclear foci formation (immunofluorescence), ionizing radiation treatment, ATM kinase assay Nature High 18449195
2009 hSSB1 (NABP2) forms a stable complex with INTS3 and hSSBIP1 (C9ORF80), distinct from the hSSB2 complex. INTS3 depletion decreases the stability of hSSB1 and hSSBIP1, suggesting INTS3 provides a scaffold for proper complex assembly. Both complexes are required for efficient HR-dependent DSB repair and ATM-dependent signaling. Immunoprecipitation, siRNA depletion, cell survival assays, HR reporter assay, ATM phosphorylation assays The Journal of biological chemistry High 19605351
2009 hSSB1 was shown by tandem affinity purification to copurify with a subset of Integrator complex subunits and MISE (c9orf80/LOC58493). The INTS3-MISE-hSSB1 complex controls ATM activation and RAD51 recruitment to DNA damage foci. INTS3 regulates hSSB1 transcription, thereby controlling hSSB1 function. Tandem affinity purification of hSSB1 phosphomimetic and phospho-null mutants, mass spectrometry, siRNA depletion, immunofluorescence for RAD51 foci The Journal of cell biology High 19786574
2010 hSSB1 is rapidly recruited to DSB sites in all interphase cell cycle phases independently of CtIP, MDC1, and the MRN complex. However, hSSB1 depletion prevents MRN complex foci formation and recruitment to DSBs, and leads to defective DSB resection (loss of RPA foci and ssDNA generation), placing hSSB1 upstream of MRN in the DSB response pathway. siRNA depletion, laser microirradiation, live-cell and fixed immunofluorescence, BrdU ssDNA detection, RPA foci assay Nucleic acids research High 21051358
2011 hSSB1 directly binds NBS1 (a component of the MRN complex) in a DNA damage-independent manner, and greatly stimulates MRN endonuclease activity in vitro via its C-terminal tail. NBS1 mutations associated with Nijmegen breakage syndrome show weaker binding to hSSB1. Direct binding assays (pulldown), in vitro endonuclease assay with purified proteins, C-terminal deletion mutants, NBS1 disease-associated mutants Nucleic acids research High 21227926
2011 hSSB1 directly binds p21 protein and this interaction prevents p21 from ubiquitin-mediated degradation. hSSB1 knockdown-induced abrogation of G1/S and G2/M checkpoints is partially dependent on p21. Co-immunoprecipitation, siRNA knockdown, cell cycle analysis, ubiquitination assay Oncogene Medium 21242961
2012 hSSB1 interacts with p53 and protects it from ubiquitin-mediated degradation. hSSB1 also associates with the acetyltransferase p300 and is required for efficient p53 acetylation at lysine 382, promoting transcriptional activation of p21. hSSB1 knockdown-induced G2/M checkpoint abrogation is partially dependent on p53 or p300. Co-immunoprecipitation, ubiquitination assays, acetylation assays, siRNA knockdown, luciferase reporter assay for p21 transcription, cell cycle analysis Cell research Medium 23184057
2013 The core hSSB1 complex contains INTS6 in addition to INTS3 and C9orf80. INTS6 directly interacts with INTS3 and forms a stable trimeric complex with INTS3 and hSSB1 both in vitro and in vivo. This complex regulates accumulation of RAD51 and BRCA1 at DNA damage sites and homologous recombination. Protein affinity purification, Co-immunoprecipitation, in vitro complex reconstitution, siRNA depletion, immunofluorescence for RAD51/BRCA1 foci, HR assay Journal of cell science Medium 23986477
2014 hSSB1 (NABP2) is required for ATR and Chk1 activation and recruitment of Mre11 and Rad51 at hydroxyurea-damaged replication forks. hSSB1-depleted cells fail to repair and restart stalled replication forks, accumulate DNA strand breaks and chromosome aberrations. siRNA depletion, hydroxyurea/camptothecin treatment, immunofluorescence for replication fork markers, Chk1/ATR phosphorylation assays, DNA fiber assay, chromosome analysis Nucleic acids research High 24753408
2014 FBXL5-containing SCF E3 ubiquitin ligase targets hSSB1 for ubiquitination and proteasomal degradation. ATM-mediated phosphorylation of hSSB1 at T117 prevents FBXL5-mediated degradation. Fbxl5 interacts directly with hSSB1. Co-immunoprecipitation, ubiquitination assays, proteasome inhibitor experiments, ATM kinase assay, overexpression/depletion studies Nucleic acids research High 25249620
2015 hSSB1 (NABP2) forms a complex with hOGG1 (human 8-oxoguanine glycosylase 1) and is required for hOGG1 localization to damaged chromatin. In vitro, hSSB1 directly binds DNA containing 8-oxoguanines and enhances hOGG1 enzymatic activity, establishing hSSB1 as a participant in base excision repair of oxidative DNA lesions. Co-immunoprecipitation, siRNA depletion, chromatin fractionation, in vitro DNA binding assays (EMSA), in vitro OGG1 activity assay with purified proteins Nucleic acids research High 26261212
2015 SSB1 (NABP2) binds specifically to G-strand telomeric DNA in vitro and associates with telomeres in vivo. SSB1 interacts with the TERT catalytic subunit and regulates its recruitment to telomeres. SSB1 deletion reduces TERT interaction with telomeres and leads to G-overhang loss. In vitro DNA binding assay (EMSA with telomeric oligonucleotides), Co-immunoprecipitation (SSB1-TERT), ChIP at telomeres, SSB1 deletion/depletion experiments, telomere G-overhang assay Cancer research Medium 25589350
2016 hSSB1 forms stable oligomers under oxidizing conditions, and this oligomerization is required for its function in base excision repair (8-oxoguanine removal) but is not required for DSB repair by homologous recombination. Monomeric hSSB1 shows decreased affinity for oxidized DNA. Size exclusion chromatography, EMSA with oxidized DNA, siRNA rescue experiments with oligomerization-deficient mutants, 8-oxoG repair assay, HR assay Scientific reports Medium 27273218
2016 ssDNA recognition by hSSB1 in solution is mediated by base-stacking of four key aromatic residues within the OB domain. This DNA binding mode in solution differs from that observed in the crystal structure of the SOSS1 complex. Solution-state NMR (chemical shift perturbation mapping), biophysical assays (ITC, fluorescence), functional DNA binding assays, comparison with crystal structure Nucleic acids research High 27387285
2017 hSSB1 forms a tetramer structurally similar to E. coli SSB, stabilized by cysteines C81 and C99 and charged/hydrophobic residues. The tetramer can still interact with INTS3, showing oligomerization does not preclude SOSS1 complex formation. Solution-state NMR, size exclusion chromatography, mutagenesis of C81/C99, Co-immunoprecipitation with INTS3, biophysical experiments Nucleic acids research High 28609781
2017 hSSB1 is directly phosphorylated by DNA-PK at serine residue 134. This modification is suppressed in undamaged cells by PPP-family protein phosphatases but is enhanced following replication fork disruption, promoting cellular survival. In vitro kinase assay with purified DNA-PK, phospho-specific antibodies, siRNA depletion of phosphatases, site-directed mutagenesis (S134A), cell survival assays after HU treatment DNA repair High 28448822
2017 hSSB1 forms a complex with BLM helicase in cells, and this interaction is altered in response to ionizing radiation. hSSB1 depletion leads to proteasome-mediated degradation of BLM and defective recruitment of BLM to chromatin following IR-induced DSBs and HU-induced stalled replication forks. Co-immunoprecipitation, siRNA depletion, proteasome inhibitor treatment, chromatin fractionation, immunofluorescence for BLM foci BMC molecular biology Medium 28506294
2015 The C-terminal tail of hSSB1 (NABP2) is essential for DNA binding ability and protein stability. Deletion of the C-terminus diminishes DNA binding. Both hNABP1 and hNABP2 exist as monomers in solution under the tested conditions, and hNABP2 has a disordered C-terminus. EMSA, ITC, circular dichroism spectroscopy, sucrose gradient centrifugation, C-terminal deletion mutants Biochimica et biophysica acta Medium 26550690
2015 When RPA is depleted, hSSB1/hSSB2 and their partner INTS3 form sub-nuclear foci, associate with the ATR-ATRIP complex, and recruit it to sites of genomic stress. This alternate ATR activation via hSSB1/2-INTS3 requires TopBP1 and the Rad9-Rad1-Hus1 complex. siRNA depletion (RPA, hSSB1/2, INTS3), immunofluorescence for ATRIP/ATR foci, Co-immunoprecipitation, Chk1 phosphorylation assay Nucleic acids research Medium 25916848
2020 hSSB1 undergoes SUMOylation at K79 and K94, enhanced by DNA damage, catalyzed by PIAS2α and reversed by SENP2. SUMOylation stabilizes hSSB1 protein and enhances recruitment of NBS1 to DNA damage sites. Cells with SUMOylation-defective hSSB1 are sensitive to ionizing radiation. In vivo SUMOylation assay, site-directed mutagenesis (K79R, K94R), Co-immunoprecipitation with PIAS2α/SENP2, NBS1 foci immunofluorescence, cell survival assay after IR, UBC9 knockout Signal transduction and targeted therapy High 32576812

Source papers

Stage 0 corpus · 61 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2008 Single-stranded DNA-binding protein hSSB1 is critical for genomic stability. Nature 199 18449195
1987 Saccharomyces cerevisiae SSB1 protein and its relationship to nucleolar RNA-binding proteins. Molecular and cellular biology 111 2823109
2009 HSSB1 and hSSB2 form similar multiprotein complexes that participate in DNA damage response. The Journal of biological chemistry 95 19605351
1984 Characterization of the structural and functional defect in the Escherichia coli single-stranded DNA binding protein encoded by the ssb-1 mutant gene. Expression of the ssb-1 gene under lambda pL regulation. The Journal of biological chemistry 95 6384214
1990 SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs. The Journal of cell biology 82 2121740
2009 INTS3 controls the hSSB1-mediated DNA damage response. The Journal of cell biology 80 19786574
1991 Monomer-tetramer equilibrium of the Escherichia coli ssb-1 mutant single strand binding protein. The Journal of biological chemistry 70 1988441
2010 hSSB1 rapidly binds at the sites of DNA double-strand breaks and is required for the efficient recruitment of the MRN complex. Nucleic acids research 64 21051358
2012 hSSB1 regulates both the stability and the transcriptional activity of p53. Cell research 63 23184057
2011 hSSB1 interacts directly with the MRN complex stimulating its recruitment to DNA double-strand breaks and its endo-nuclease activity. Nucleic acids research 61 21227926
2011 hSSB1 binds and protects p21 from ubiquitin-mediated degradation and positively correlates with p21 in human hepatocellular carcinomas. Oncogene 54 21242961
2004 The Reg1-interacting proteins, Bmh1, Bmh2, Ssb1, and Ssb2, have roles in maintaining glucose repression in Saccharomyces cerevisiae. The Journal of biological chemistry 53 15220335
2014 Human single-stranded DNA binding protein 1 (hSSB1/NABP2) is required for the stability and repair of stalled replication forks. Nucleic acids research 48 24753408
1982 Effects of the ssb-1 and ssb-113 mutations on survival and DNA repair in UV-irradiated delta uvrB strains of Escherichia coli K-12. Journal of bacteriology 47 7045074
2005 The SPRY domain-containing SOCS box protein 1 (SSB-1) interacts with MET and enhances the hepatocyte growth factor-induced Erk-Elk-1-serum response element pathway. The Journal of biological chemistry 46 15713673
2001 Ssb1 chaperone is a [PSI+] prion-curing factor. Current genetics 46 11405097
1983 Amplification of ssb-1 mutant single-stranded DNA-binding protein in Escherichia coli. Journal of molecular biology 46 6341603
2013 A core hSSB1-INTS complex participates in the DNA damage response. Journal of cell science 44 23986477
1991 Monomers of the Escherichia coli SSB-1 mutant protein bind single-stranded DNA. Journal of molecular biology 41 1988680
2015 hSSB1 (NABP2/ OBFC2B) is required for the repair of 8-oxo-guanine by the hOGG1-mediated base excision repair pathway. Nucleic acids research 39 26261212
1981 Variable expression of the ssb--1 allele in different strains of Escherichia coli K12 and B: differential suppression of its effects on DNA replication, DNA repair and ultraviolet mutagenesis. Molecular & general genetics : MGG 37 6276686
2014 FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response. Nucleic acids research 35 25249620
1997 Modulation of intracellular protein degradation by SSB1-SIS1 chaperon system in yeast S. cerevisiae. FEBS letters 35 9202167
2020 SUMOylation stabilizes hSSB1 and enhances the recruitment of NBS1 to DNA damage sites. Signal transduction and targeted therapy 33 32576812
2013 Essential developmental, genomic stability, and tumour suppressor functions of the mouse orthologue of hSSB1/NABP2. PLoS genetics 31 23408915
2012 The hSSB1 orthologue Obfc2b is essential for skeletogenesis but dispensable for the DNA damage response in vivo. The EMBO journal 29 22940690
1982 Suppression of the ssb-1 and ssb-113 mutations of Escherichia coli by a wild-type rep gene, NaCl, and glucose. Journal of bacteriology 29 6752116
2018 Human single-stranded DNA binding protein 1 (hSSB1, OBFC2B), a critical component of the DNA damage response. Seminars in cell & developmental biology 27 29577982
2016 hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress. Scientific reports 26 27273218
2016 A structural analysis of DNA binding by hSSB1 (NABP2/OBFC2B) in solution. Nucleic acids research 24 27387285
2015 Single-strand DNA-binding protein SSB1 facilitates TERT recruitment to telomeres and maintains telomere G-overhangs. Cancer research 20 25589350
2017 Ssb1 and Ssb2 cooperate to regulate mouse hematopoietic stem and progenitor cells by resolving replicative stress. Blood 18 28270450
2015 Improvement of lactic acid production in Saccharomyces cerevisiae by a deletion of ssb1. Journal of industrial microbiology & biotechnology 17 26660479
1988 Suppression of the Escherichia coli ssb-1 mutation by an allele of groEL. Proceedings of the National Academy of Sciences of the United States of America 17 2897690
2021 Genome-wide association study in the Taiwan Biobank identifies four novel genes for human height: NABP2, RASA2, RNF41 and SLC39A5. Human molecular genetics 15 34270706
1997 Molecular cloning of a Candida albicans gene (SSB1) coding for a protein related to the Hsp70 family. Yeast (Chichester, England) 15 9200817
2017 hSSB1 phosphorylation is dynamically regulated by DNA-PK and PPP-family protein phosphatases. DNA repair 14 28448822
2000 Complementation of Saccharomyces cerevisiae mutations in genes involved in translation and protein folding (EFB1 and SSB1) with Candida albicans cloned genes. Research in microbiology 13 11130864
2017 A data-driven structural model of hSSB1 (NABP2/OBFC2B) self-oligomerization. Nucleic acids research 12 28609781
2009 Cloning and characterization of the SSB-1 and SSB-4 genes expressed in zebrafish gonads. Biochemical genetics 10 19184407
1987 Differential suppressor effects of the ssb-1 and ssb-113 alleles on uvrD mutator of Escherichia coli in DNA repair and mutagenesis. Journal of basic microbiology 10 2964522
2023 hSSB1 (NABP2/OBFC2B) modulates the DNA damage and androgen-induced transcriptional response in prostate cancer. The Prostate 9 36811381
2019 A Structural Perspective on the Regulation of Human Single-Stranded DNA Binding Protein 1 (hSSB1, OBFC2B) Function in DNA Repair. Computational and structural biotechnology journal 9 30996823
2017 hSSB1 associates with and promotes stability of the BLM helicase. BMC molecular biology 9 28506294
2015 RPA70 depletion induces hSSB1/2-INTS3 complex to initiate ATR signaling. Nucleic acids research 9 25916848
1994 A 21.7 kb DNA segment on the left arm of yeast chromosome XIV carries WHI3, GCR2, SPX18, SPX19, an homologue to the heat shock gene SSB1 and 8 new open reading frames of unknown function. Yeast (Chichester, England) 9 7725799
2016 Novel insight into the composition of human single-stranded DNA-binding protein 1 (hSSB1)-containing protein complexes. BMC molecular biology 8 27938330
2015 C-termini are essential and distinct for nucleic acid binding of human NABP1 and NABP2. Biochimica et biophysica acta 8 26550690
2019 SSB1/SSB2 Proteins Safeguard B Cell Development by Protecting the Genomes of B Cell Precursors. Journal of immunology (Baltimore, Md. : 1950) 6 31085591
2016 Backbone (1)H, (13)C and (15)N resonance assignments of the OB domain of the single stranded DNA-binding protein hSSB1 (NABP2/OBFC2B) and chemical shift mapping of the DNA-binding interface. Biomolecular NMR assignments 6 27193589
2006 Identification of the divergent calmodulin binding motif in yeast Ssb1/Hsp75 protein and in other HSP70 family members. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 4 17146552
2023 Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeast. PLoS genetics 3 37200372
1992 Purification of ribonucleoproteins by a novel approach: isolation of the SSB1 ribonucleoprotein from yeast and demonstration that it has no role in mRNA splicing. Biochimica et biophysica acta 2 1482680
2024 Effect of silencing SSB1 gene on the expression of NBS1 in irradiated rat submandibular gland cells. Cellular and molecular biology (Noisy-le-Grand, France) 1 38372104
2024 Targeting the hSSB1-INTS3 Interface: A Computational Screening Driven Approach to Identify Potential Modulators. ACS omega 1 38405517
2023 NABP2 as an oncogenic biomarker promotes hepatocellular carcinoma progression and metastasis. American journal of translational research 1 37434816
2026 Expanding the Fish-Brain Invitrome With the Senegalese Sole SsB-1 Cell Line-A Versatile Model for Neurotropic Virus Research. Journal of fish diseases 0 42046907
2025 In Saccharomyces cerevisiae, the molecular chaperone proteins Ssb1 and Ssb2 upregulate ABC transporter genes, and their upregulation may play a role in the release of quorum-sensing molecules that induce cell growth arrest during the diauxic shift. AIMS microbiology 0 41078542
2024 The single-strand DNA-binding protein SSB1 is involved in the expression of salivary gland radiation injury repair. Frontiers in pharmacology 0 39474611
2023 Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeast. bioRxiv : the preprint server for biology 0 36945624
2021 Expression, Purification, and Solution-State NMR Analysis of the Two Human Single-Stranded DNA-Binding Proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A). Methods in molecular biology (Clifton, N.J.) 0 33847962

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