Affinage

MPHOSPH10

U3 small nucleolar ribonucleoprotein protein MPP10 · UniProt O00566

Round 2 corrected
Length
681 aa
Mass
78.9 kDa
Annotated
2026-04-28
78 papers in source corpus 22 papers cited in narrative 22 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MPHOSPH10 (MPP10) is an essential nucleolar component of the U3 small nucleolar ribonucleoprotein (snoRNP) and the 90S pre-ribosomal SSU processome, where it functions as a multi-partner interaction platform required for pre-rRNA cleavage at sites A0, A1, and A2 during 18S rRNA maturation (PMID:9315638, PMID:28967883). MPP10 forms a discrete Mpp10–Imp3–Imp4 sub-complex that is recruited to nascent pre-rRNA after the 50S U3 snoRNP intermediate docks on the transcript; its association with U3 snoRNA depends on a conserved hinge-region element, and its intact C-terminus is specifically required for cleavage at sites A1 and A2 (PMID:9397061, PMID:11421365, PMID:19332556). Structurally, MPP10 bridges multiple processome modules—binding Imp4, Sas10/UTP3, and ribosomal protein uS7—and stabilizes U3 snoRNA–pre-rRNA heteroduplexes near the A1 cleavage site (PMID:28813493, PMID:28967883). MPP10 protein levels and nucleolar delivery are regulated by UTP3/Sas10, which both ferries MPP10 into the nucleolus via importin-α and shields it from Def–CAPN3-mediated proteolytic degradation (PMID:30773582, PMID:39036955).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1996 High

    Identifying MPP10 as an M-phase phosphoprotein with nucleolar localization established it as a candidate ribosome biogenesis factor rather than a generic mitotic phosphoprotein.

    Evidence Expression cloning and immunofluorescence in human cells

    PMID:8885239

    Open questions at the time
    • Phosphorylation function unknown
    • No connection to rRNA processing yet established
  2. 1997 High

    Demonstrating that yeast Mpp10p is an essential U3 snoRNP component required for pre-rRNA cleavage at A0, A1, and A2 sites defined its core function in 18S rRNA biogenesis, while truncation mutagenesis separated its A0 function from a specific C-terminal requirement for A1/A2 cleavage.

    Evidence Conditional depletion, Northern blot and pulse-chase rRNA analysis, co-IP of U3 snoRNA, C-terminal truncation mutagenesis in yeast

    PMID:9315638 PMID:9391061

    Open questions at the time
    • Mechanism by which the C-terminus promotes A1/A2 cleavage unresolved
    • Direct protein partners unknown
  3. 1998 High

    Localization of human MPP10 to the nucleolar fibrillar component and demonstration of its salt-stable, U3-specific association confirmed conservation of the U3 snoRNP role in mammals.

    Evidence Cell fractionation, immunofluorescence, co-IP of snoRNAs in human cells

    PMID:9450966

    Open questions at the time
    • Identity of human MPP10 binding partners not yet determined
    • Functional requirement in human pre-rRNA processing not tested
  4. 1999 High

    Discovery of Imp3p and Imp4p as U3 snoRNP-specific partners of Mpp10p revealed a dedicated sub-complex essential for A0/A1/A2 cleavage, answering how Mpp10p is embedded in the processome.

    Evidence Yeast two-hybrid with Mpp10p bait, co-IP, genetic depletion

    PMID:10409734

    Open questions at the time
    • Structural basis of the Mpp10–Imp3–Imp4 interaction unknown
    • Whether the sub-complex acts as a unit or each member has independent functions unclear
  5. 2001 High

    Mapping Mpp10p association to the conserved U3 snoRNA hinge region positioned Mpp10p near the functional 5′ domain that base-pairs with pre-rRNA, linking its binding site to catalytic geography.

    Evidence Systematic U3 snoRNA deletion/mutation analysis with co-IP readout in yeast

    PMID:11421365

    Open questions at the time
    • Atomic-resolution contacts between Mpp10p and U3 hinge not resolved
    • Whether Mpp10p contacts pre-rRNA directly unknown
  6. 2002 High

    Genetic epistasis between Mpp10p truncations and U3 snoRNA mutations producing additive A2 defects established that Mpp10p contributes to processing beyond simply facilitating U3–pre-rRNA base pairing.

    Evidence Double-mutant epistasis analysis with pre-rRNA processing readouts in yeast

    PMID:12242301

    Open questions at the time
    • Nature of the 'beyond base-pairing' function not molecularly defined
    • Whether Mpp10p contacts the endonuclease directly unknown
  7. 2004 High

    Linking Mpp10p recruitment to the 80S SSU processome with subnucleolar relocalization of U3 snoRNA from the dense fibrillar component to the granular component showed that Mpp10p association is a regulated, spatially coupled step in processome maturation.

    Evidence U3 snoRNA mutation analysis, sucrose gradient sedimentation, immunofluorescence in yeast

    PMID:15367679

    Open questions at the time
    • Signal or checkpoint that triggers relocation not identified
    • Dynamics of Mpp10p recycling unknown
  8. 2007 High

    Hierarchical assembly mapping of the 90S pre-ribosome placed the Mpp10p-containing module downstream of UtpA (t-UTP) engagement, establishing the temporal order of processome assembly.

    Evidence Co-IP, MudPIT quantitative proteomics, and bioinformatic topology modeling in yeast

    PMID:17515605

    Open questions at the time
    • Kinetics of Mpp10 module joining in real time not measured
    • Whether assembly order is conserved in human cells untested at this point
  9. 2009 High

    Discovery that MPP10 is absent from a human 50S U3 snoRNP intermediate confirmed that, as in yeast, MPP10 is recruited only after U3 snoRNP docks on pre-rRNA, validating a conserved co-transcriptional assembly mechanism.

    Evidence Actinomycin D block, tUTP siRNA depletion, sucrose gradient sedimentation, co-IP in human cells

    PMID:19332556

    Open questions at the time
    • Signals for MPP10 recruitment to the docked U3 complex not identified
  10. 2016 High

    Cryo-EM visualization of the intact 90S pre-ribosome placed the Mpp10–Imp3–Imp4 module as a discrete structural unit within the particle, providing the first direct structural evidence of its spatial organization relative to U3 snoRNA and pre-rRNA.

    Evidence Cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome

    PMID:27419870

    Open questions at the time
    • Resolution insufficient for atomic contacts at this stage
    • Conformational dynamics of the module during processing unresolved
  11. 2017 High

    Near-atomic cryo-EM and crystal structures revealed how Mpp10 stabilizes U3 snoRNA–pre-rRNA heteroduplexes and positions the A1 site near the Utp24 endonuclease, while crystallography of Imp4–Mpp10 and interaction mapping to Sas10/UTP3 and uS7 defined Mpp10 as a multi-valent interaction hub.

    Evidence 3.2-Å cryo-EM with atomic model building; 1.88-Å crystal structure of Imp4–Mpp10 peptide complex; GST pulldowns and two-hybrid assays

    PMID:28813493 PMID:28967883

    Open questions at the time
    • Conformational changes during catalytic activation not captured
    • Whether Mpp10 contacts Utp24 directly remains unresolved
  12. 2019 High

    Identification of the Def–CAPN3 protease pathway as a regulator of Mpp10 stability, with Sas10/UTP3 acting as a protective shield against CAPN3-mediated cleavage, revealed how Mpp10 turnover is coupled to processome assembly and organismal development.

    Evidence Zebrafish genetic loss-of-function, co-IP, protein stability and protease protection assays, nucleolar localization imaging

    PMID:30773582

    Open questions at the time
    • CAPN3 cleavage site on Mpp10 not mapped at residue level
    • Whether this regulatory axis operates identically in human cells not directly shown
  13. 2024 High

    A systematic screen demonstrated that UTP3/Sas10 ferries MPP10 into the nucleolus via importin-α, directly linking MPP10 nucleolar import to its protective partner and to 5′-ETS processing competence.

    Evidence siRNA screen of 50 SSU processome components, live-cell nucleolar imaging, importin-α interaction assays, zebrafish LOF, Northern blot in human cells and zebrafish

    PMID:39036955

    Open questions at the time
    • NLS on MPP10 or UTP3 responsible for importin-α recognition not mapped
    • Whether additional chaperones are required for nucleolar import unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • Major open questions include the precise cleavage site(s) on Mpp10 recognized by CAPN3, the structural basis of Mpp10 conformational changes during catalytic activation of the processome, and whether Mpp10 phosphorylation (the original basis of its discovery) regulates its processome association or turnover.
  • Functional significance of M-phase phosphorylation never resolved
  • No reconstituted in vitro processing assay with defined Mpp10 mutants
  • Dynamic structural intermediates of Mpp10 during rRNA cleavage not captured

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 4 GO:0005198 structural molecule activity 3
Localization
GO:0005730 nucleolus 5 GO:0005634 nucleus 1
Pathway
R-HSA-8953854 Metabolism of RNA 8 R-HSA-392499 Metabolism of proteins 3
Partners
CAPN3DEFIMP3IMP4RPS5UTP11UTP3
Complex memberships
Mpp10–Imp3–Imp4 sub-complexSSU processome (90S pre-ribosome)U3 snoRNP

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1996 MPP10 (MPHOSPH10) was identified as an M phase phosphoprotein that localizes to nucleoli in interphase cells and redistributes to chromosome surfaces and prenucleolar/nucleolus-derived bodies during mitosis, as determined by immunofluorescence microscopy with affinity-purified antibodies. Expression cloning, immunofluorescence microscopy, immunoprecipitation with MPM2 antibody Molecular biology of the cell High 8885239
1997 Yeast Mpp10p is a protein component of the U3 snoRNP (co-immunoprecipitates U3 snoRNA), MPP10 is an essential gene, and depletion of Mpp10p causes accumulation of 23S and 35S pre-rRNA precursors with reduction of 18S rRNA and its 20S precursor, demonstrating that Mpp10p is required for pre-rRNA cleavage at sites A0, A1, and A2. Immunoprecipitation of U3 snoRNA, conditional (GAL-promoter) depletion, Northern blot analysis of pre-rRNA processing, pulse-chase rRNA analysis Molecular and cellular biology High 9315638
1997 Truncation mutations of yeast Mpp10p that remove terminal charged domains separate U3 snoRNP function: truncated Mpp10p supports cleavage at site A0 but not at A1/A2, without affecting U3 snoRNA or protein stability or Mpp10p–U3 snoRNA association, revealing a specific role for the intact C-terminus in A1/A2 processing. Site-directed truncation mutagenesis, Northern blot pre-rRNA processing analysis, immunoprecipitation of U3 snoRNA Proceedings of the National Academy of Sciences of the United States of America High 9391061
1998 Human MPP10 localizes almost exclusively to the nucleolar fibrillar component (fibrillar caps) by cell fractionation and immunofluorescence, co-localizes with fibrillarin in interphase and on chromosome surfaces in mitosis, and co-immunoprecipitates U3 snoRNA specifically (stable to 400 mM salt) but not other box C/D snoRNAs, establishing it as a human U3 snoRNP component. Cell fractionation, immunofluorescence microscopy, actinomycin D treatment to separate nucleolar domains, immunoprecipitation of snoRNAs Molecular biology of the cell High 9450966
1999 Imp3p and Imp4p are novel U3 snoRNP-specific proteins identified by two-hybrid screening with Mpp10p as bait; both physically associate with Mpp10p in vivo, are exclusively complexed with U3 snoRNA, and are essential for pre-rRNA cleavage at A0, A1, and A2 sites, without affecting U3 snoRNA integrity. Yeast two-hybrid screen (bait: Mpp10p), co-immunoprecipitation with Mpp10p, Northern blot of pre-rRNA, genetic depletion Molecular and cellular biology High 10409734
2001 Association of Mpp10p with the U3 snoRNP requires a conserved sequence element in the U3 snoRNA hinge region (not the 3' domain sufficient for fibrillarin and other components), placing Mpp10p near the 5' domain that mediates pre-rRNA base-pairing interactions. U3 snoRNA deletion/mutation analysis, immunoprecipitation of Mpp10p with U3 snoRNA mutants RNA (New York, N.Y.) High 11421365
2002 Imp3p, Imp4p, and Mpp10p form an interdependent unit within the SSU processome: depletion of any one destabilizes the others' association with U3 snoRNA; C-terminal truncations of Mpp10p combined with U3 snoRNA mutations that disrupt pre-rRNA processing produce additive defects at site A2, indicating Mpp10p also functions at a step beyond U3–pre-rRNA base pairing. Genetic depletion, U3 snoRNA–Mpp10p co-immunoprecipitation, double-mutant (epistasis) growth and pre-rRNA processing analysis, heterologous complementation Molecular and cellular biology High 12242301
2003 Human MPP10 is an autoantigen in scleroderma; anti-Mpp10 autoantibodies are found in scleroderma patients (predominantly antifibrillarin-positive), confirming that human MPP10 is a genuine U3 snoRNP component recognized as an immunogenic nucleolar antigen. Serum immunoprecipitation / immunoblotting of MPP10 from patient sera, clinical correlation Arthritis and rheumatism Medium 12528121
2004 Binding of Mpp10p to the 80S U3 pre-rRNA processing complex (SSU processome) depends on U3 snoRNA sequences that base-pair with pre-rRNA adjacent to the initial cleavage site; mutations blocking 80S complex formation or Mpp10p association cause retention of U3 snoRNA in the dense fibrillar component, showing that Mpp10p recruitment drives movement of U3 snoRNA from DFC to the granular component of the nucleolus. U3 snoRNA mutation analysis, sucrose gradient sedimentation, immunoprecipitation (Mpp10p association with 80S complex), immunofluorescence Molecular and cellular biology High 15367679
2004 Imp4p mutations that disrupt the two-hybrid interaction with Mpp10p confer conditional pre-rRNA processing defects in vivo but are not measurably defective for Mpp10p interaction within the intact SSU processome, suggesting other complex members stabilize the Mpp10p–Imp4p interaction in the large RNP. Reverse two-hybrid screen, in vivo co-immunoprecipitation within the SSU processome, pre-rRNA Northern blot Nucleic acids research Medium 14990745
2007 The yeast 90S preribosome is assembled in a hierarchical, modular manner: assembly of the t-UTP (UtpA) subunit is a prerequisite for engagement of at least five additional subcomplexes including the Mpp10p-containing complex and U3 snoRNP, in two mutually independent routes, defining the topological position of the Mpp10 module within the fully assembled particle. Biochemical fractionation, co-immunoprecipitation, quantitative proteomics (MudPIT), bioinformatic topology modeling Molecular and cellular biology High 17515605
2009 In human cells, the SSU processome is dynamic: the MPP10 subcomplex (together with tUTP, bUTP, and BMS1/RCL1) is absent from a novel 50S U3 snoRNP assembly intermediate that accumulates when pre-rRNA transcription is blocked or tUTP proteins are depleted, indicating MPP10 is recruited to the processome only after the 50S U3 snoRNP docks on pre-rRNA. Pre-rRNA transcription block (actinomycin D), siRNA depletion of tUTP, sucrose gradient sedimentation, co-immunoprecipitation Molecular and cellular biology High 19332556
2016 Cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome identifies the Mpp10–Imp3–Imp4 module as a discrete structural unit positioned within the particle, engaged with the pre-rRNA alongside the U3 snoRNP, UTP-A, UTP-B, and Bms1–Rcl1 modules. Cryo-EM structure determination of intact 90S pre-ribosome Cell High 27419870
2016 Mpp10 is identified as a substrate of the yeast arginine methyltransferase Hmt1 by proteome microarray and validated by ex vivo methylation and MS/MS, adding arginine methylation to the post-translational modification repertoire of Mpp10. Proteome microarray with anti-methylarginine antibody, recombinant Hmt1 treatment, ex vivo methylation assay, MS/MS validation Proteomics Medium 26572822
2017 3.2-Å cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome allowed atomic model building for 34 assembly factors including the Mpp10 complex; the structure reveals how Mpp10 and associated factors stabilize U3 RNA heteroduplexes with the 5'-ETS and pre-18S RNA, and positions the unprocessed A1 cleavage site near the Utp24 endonuclease. High-resolution cryo-EM (3.2 Å) with atomic model building Nature structural & molecular biology High 28967883
2017 Mpp10 serves as a protein-interaction platform in the 90S pre-ribosome: crystal structure of an Imp4–Mpp10 peptide complex (1.88 Å) was solved; additional interactions were characterized showing Mpp10 binds Utp3/Sas10 via two conserved N-terminal motifs and also contacts ribosomal protein S5/uS7 via an acidic loop region, enabling simultaneous engagement with multiple partners. Crystal structure determination (1.88 Å), GST pulldown assays, yeast two-hybrid PloS one High 28813493
2019 Sas10/Utp3 controls ribosome biogenesis by (1) protecting Mpp10 from cleavage by the nucleolus-localized cysteine proteinase Capn3 (by masking the Capn3 recognition site), while Def promotes Capn3-mediated Mpp10 cleavage; and (2) determining the nucleolar localization of the Mpp10–Imp3–Imp4 complex. These roles were demonstrated in zebrafish as essential for digestive organ development. Zebrafish genetic loss-of-function, co-immunoprecipitation, protein stability assays, nucleolar localization imaging, in vivo protease protection assay Nucleic acids research High 30773582
2021 Mpp10 is a substrate of the nucleolus-localized Def-CAPN3 (cysteine proteinase) protein degradation pathway; loss of Def causes nucleolar accumulation of Mpp10 and consequent rRNA processing abnormality and cell-cycle arrest, demonstrating that regulated Mpp10 turnover is required for normal ribosome biogenesis and cell-cycle progression. Review summarizing zebrafish and human genetic/biochemical studies; Def depletion phenotype analysis, immunofluorescence, rRNA processing assays Journal of genetics and genomics Medium 34452850
2022 An 86-amino acid motif (residues 430–515) in human CAPN3 is essential for interaction with human Def, and the 2/3 C-terminus of Def mediates the Def–CAPN3 interaction; these interaction domains are conserved in zebrafish. This defines the structural basis for formation of the Def-CAPN3 complex that degrades Mpp10 in the nucleolus. Truncation/deletion mutagenesis of CAPN3 and Def, co-immunoprecipitation Biochemical and biophysical research communications Medium 35878425
2023 UTP11 binds to MPP10 (human MPHOSPH10) and is required for 18S rRNA biosynthesis; UTP11 depletion impairs 18S rRNA biogenesis and triggers nucleolar stress (stabilizing p53 via RPL5/RPL11) and ferroptosis (via NRF2/SLC7A11 axis), demonstrating that the UTP11–MPP10 interaction is integral to SSU processome-dependent rRNA processing. Co-immunoprecipitation, siRNA knockdown, Northern blot (rRNA processing), xenograft tumor models, p53 stabilization assays Redox biology Medium 37087976
2024 UTP3/SAS10 facilitates nucleolar import of human MPP10 (along with UTP25, EMG1, and UTP-B components UTP12/UTP13) by a 'ferrying' mechanism likely involving interaction with nuclear importin α; UTP3 knockdown impairs A0-site cleavage of pre-rRNA, and loss of utp3/sas10 in zebrafish causes accumulation of aberrantly processed 5'-ETS products, functionally linking MPP10 nucleolar entry to 5'-ETS processing. Systematic siRNA knockdown screen of 50 human SSU processome components, live-cell nucleolar localization imaging, importin-α interaction assays, zebrafish loss-of-function, Northern blot of pre-rRNA Nucleic acids research High 39036955
2020 SARS-CoV-2 Nsp8 (and other viral proteins) physically associates with human MPHOSPH10 in affinity-purification mass spectrometry experiments, identifying MPHOSPH10 as a host interactor of SARS-CoV-2. Affinity purification–mass spectrometry (AP-MS) of tagged SARS-CoV-2 proteins in HEK293T cells Nature Low 32353859

Source papers

Stage 0 corpus · 78 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2020 A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature 3411 32353859
2006 Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 2861 17081983
2012 Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell 1718 22658674
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2012 The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. Molecular cell 973 22681889
2005 Nucleolar proteome dynamics. Nature 934 15635413
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2002 Directed proteomic analysis of the human nucleolus. Current biology : CB 780 11790298
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2015 Gene essentiality and synthetic lethality in haploid human cells. Science (New York, N.Y.) 657 26472760
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2018 High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies. Molecular cell 580 29395067
2020 Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms. Science (New York, N.Y.) 564 33060197
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2005 Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome research 409 16344560
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2002 Functional proteomic analysis of human nucleolus. Molecular biology of the cell 391 12429849
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2009 Docking motif-guided mapping of the interactome of protein phosphatase-1. Chemistry & biology 269 19389623
2016 The cell proliferation antigen Ki-67 organises heterochromatin. eLife 265 26949251
2004 Functional proteomics mapping of a human signaling pathway. Genome research 247 15231748
2018 An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations. Nature communications 201 29568061
2016 Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome. Cell 181 27419870
1996 Identification of novel M phase phosphoproteins by expression cloning. Molecular biology of the cell 168 8885239
2020 A High-Density Human Mitochondrial Proximity Interaction Network. Cell metabolism 148 32877691
2012 Functional proteomics establishes the interaction of SIRT7 with chromatin remodeling complexes and expands its role in regulation of RNA polymerase I transcription. Molecular & cellular proteomics : MCP 145 22586326
2007 The 90S preribosome is a multimodular structure that is assembled through a hierarchical mechanism. Molecular and cellular biology 142 17515605
2004 The small-subunit processome is a ribosome assembly intermediate. Eukaryotic cell 142 15590835
2022 A comprehensive SARS-CoV-2-human protein-protein interactome reveals COVID-19 pathobiology and potential host therapeutic targets. Nature biotechnology 140 36217030
2016 FOXA1 Directs H3K4 Monomethylation at Enhancers via Recruitment of the Methyltransferase MLL3. Cell reports 137 27926873
1999 Imp3p and Imp4p, two specific components of the U3 small nucleolar ribonucleoprotein that are essential for pre-18S rRNA processing. Molecular and cellular biology 122 10409734
1997 Mpp10p, a U3 small nucleolar ribonucleoprotein component required for pre-18S rRNA processing in yeast. Molecular and cellular biology 103 9315638
2017 3.2-Å-resolution structure of the 90S preribosome before A1 pre-rRNA cleavage. Nature structural & molecular biology 89 28967883
2009 A novel small-subunit processome assembly intermediate that contains the U3 snoRNP, nucleolin, RRP5, and DBP4. Molecular and cellular biology 59 19332556
2011 A second base pair interaction between U3 small nucleolar RNA and the 5'-ETS region is required for early cleavage of the yeast pre-ribosomal RNA. Nucleic acids research 53 21890904
2002 Components of an interdependent unit within the SSU processome regulate and mediate its activity. Molecular and cellular biology 53 12242301
1998 M phase phosphoprotein 10 is a human U3 small nucleolar ribonucleoprotein component. Molecular biology of the cell 50 9450966
2017 Monocytes inhibit NK activity via TGF-β in patients with obstructive sleep apnoea. The European respiratory journal 49 28619958
2010 The DEAD-box RNA helicase-like Utp25 is an SSU processome component. RNA (New York, N.Y.) 44 20884785
1997 Functional separation of pre-rRNA processing steps revealed by truncation of the U3 small nucleolar ribonucleoprotein component, Mpp10. Proceedings of the National Academy of Sciences of the United States of America 43 9391061
2017 Hypoxia and inflammation indicate significant differences in the severity of obstructive sleep apnea within similar apnea-hypopnea index groups. Sleep & breathing = Schlaf & Atmung 42 28271327
2017 Genetics of early-onset Parkinson's disease in Finland: exome sequencing and genome-wide association study. Neurobiology of aging 41 28256260
2004 Role of pre-rRNA base pairing and 80S complex formation in subnucleolar localization of the U3 snoRNP. Molecular and cellular biology 38 15367679
2003 Human scleroderma sera contain autoantibodies to protein components specific to the U3 small nucleolar RNP complex. Arthritis and rheumatism 37 12528121
2016 Association of Diabetic Microvascular Complications and Parameters of Obstructive Sleep Apnea in Patients with Type 2 Diabetes. Diabetes technology & therapeutics 35 27031372
2004 Imp3p and Imp4p mediate formation of essential U3-precursor rRNA (pre-rRNA) duplexes, possibly to recruit the small subunit processome to the pre-rRNA. Proceedings of the National Academy of Sciences of the United States of America 35 15489263
2019 Sas10 controls ribosome biogenesis by stabilizing Mpp10 and delivering the Mpp10-Imp3-Imp4 complex to nucleolus. Nucleic acids research 27 30773582
2010 Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA. The FEBS journal 26 20528918
2013 Nucleolar proteins Bfr2 and Enp2 interact with DEAD-box RNA helicase Dbp4 in two different complexes. Nucleic acids research 23 24357410
2023 UTP11 deficiency suppresses cancer development via nucleolar stress and ferroptosis. Redox biology 21 37087976
2001 An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association. RNA (New York, N.Y.) 21 11421365
2008 Mrd1p binds to pre-rRNA early during transcription independent of U3 snoRNA and is required for compaction of the pre-rRNA into small subunit processomes. Nucleic acids research 19 18586827
2014 DEAD-box RNA helicase Dbp4 is required for small-subunit processome formation and function. Molecular and cellular biology 18 25535329
2004 Two-hybrid Mpp10p interaction-defective Imp4 proteins are not interaction defective in vivo but do confer specific pre-rRNA processing defects in Saccharomyces cerevisiae. Nucleic acids research 17 14990745
2017 Mpp10 represents a platform for the interaction of multiple factors within the 90S pre-ribosome. PloS one 15 28813493
2010 Adjacent gene pairing plays a role in the coordinated expression of ribosome biogenesis genes MPP10 and YJR003C in Saccharomyces cerevisiae. Eukaryotic cell 14 21115740
2014 Dissecting the cis and trans elements that regulate adjacent-gene coregulation in Saccharomyces cerevisiae. Eukaryotic cell 13 24706020
2006 Mutant Bik expression mediated by the enhanced minimal topoisomerase IIalpha promoter selectively suppressed breast tumors in an animal model. Cancer gene therapy 11 16514421
2022 Research on the Association Between Obstructive Sleep Apnea Hypopnea Syndrome Complicated With Coronary Heart Disease and Inflammatory Factors, Glycolipid Metabolism, Obesity, and Insulin Resistance. Frontiers in endocrinology 10 35846285
2021 Nucleolus-localized Def-CAPN3 protein degradation pathway and its role in cell cycle control and ribosome biogenesis. Journal of genetics and genomics = Yi chuan xue bao 9 34452850
2019 The influence of sleep apnea syndrome and intermittent hypoxia in carotid adventitial vasa vasorum. PloS one 9 30721271
2016 Protein substrates of the arginine methyltransferase Hmt1 identified by proteome arrays. Proteomics 9 26572822
2014 Purification, crystallization and preliminary X-ray diffraction analysis of Imp3 in complex with an Mpp10 peptide involved in yeast ribosome biogenesis. Acta crystallographica. Section F, Structural biology communications 9 25005089
1997 Mpp10p, a new protein component of the U3 snoRNP required for processing of 18S rRNA precursors. Nucleic acids symposium series 9 9478208
2022 Improved Inflammatory and Cardiometabolic Profile After Soft-Tissue Sleep Surgery for Obstructive Sleep Apnea: A Systematic Review and Meta-analysis. JAMA otolaryngology-- head & neck surgery 8 35951318
2019 Proteins and RNA sequences required for the transition of the t-Utp complex into the SSU processome. FEMS yeast research 8 30445532
2007 Panax ginseng increases hypoxia-induced down-regulated cellular response related genes in human neuroblastoma cells, SK-N-MC. Neurological research 7 17359646
2023 P66Shc is increased in peripheral blood mononuclear cells of the patients with obstructive sleep apnea. International journal of medical sciences 5 37057208
2022 Carbonic Anhydrase IX as a Marker of Disease Severity in Obstructive Sleep Apnea. Medicina (Kaunas, Lithuania) 5 36422182
2024 A transcriptome-wide association study identified susceptibility genes for hepatocellular carcinoma in East Asia. Gastroenterology report 4 38846986
2020 Anti-infective nitazoxanide disrupts transcription of ribosome biogenesis-related genes in yeast. Genes & genomics 4 32524281
2024 The Interactive Impacts of Corn Particle Size and Conditioning Temperature on Performance, Carcass Traits, and Intestinal Morphology of Broiler Chickens. Animals : an open access journal from MDPI 2 38473202
2024 A UTP3-dependent nucleolar translocation pathway facilitates pre-rRNA 5'ETS processing. Nucleic acids research 2 39036955
2022 An 86 amino acids motif in CAPN3 is essential for formation of the nucleolus-localized Def-CAPN3 complex. Biochemical and biophysical research communications 2 35878425