| 2000 |
Yeast Hrs1/Med3 (ortholog of MED27) physically interacts with the Cyc8-Tup1 corepressor via direct binding, and this interaction allows Cyc8-Tup1 to co-purify with the Mediator complex in a Hrs1-dependent manner; increased Hrs1 dosage alleviates Cyc8-Tup1-mediated repression, suggesting that Mediator function is inhibited upon this interaction, thereby preventing RNA pol II holoenzyme recruitment to the core promoter. |
Two-hybrid assay, GST pulldown, affinity chromatography, genetic dosage suppression, artificial holoenzyme recruitment assay |
The Journal of biological chemistry |
High |
10722672
|
| 2012 |
In Candida albicans, Med3 (MED27 ortholog) directly and physically interacts with Tlo proteins (Med2-module subunits); Med3 is required for Tlo incorporation into the Mediator complex, as Mediator purified from a med3Δ/Δ strain lacks Tlo subunits. |
Mediator complex purification, co-purification of tagged proteins, reconstitution of direct interaction by coexpression and copurification in E. coli |
Eukaryotic cell |
High |
22562472
|
| 2014 |
Yeast Med3 (MED27 ortholog) is phosphorylated by Cdk8 (the Mediator kinase module subunit); site-specific phosphorylation triggers interaction with the Grr1 ubiquitin ligase and subsequent Med3 degradation, thereby suppressing transcription activation; this autoregulatory mechanism explains the substoichiometric levels of tail-module Med3 in purified Mediator. |
In vitro kinase assay, site-specific mutagenesis, Co-IP/interaction assay between Med3 and Grr1, genetic analysis of cdk8 mutants and grr1 mutants, protein stability assays |
Proceedings of the National Academy of Sciences of the United States of America |
High |
24550274
|
| 2006 |
Loss of Crsp34/Med27 (zebrafish ortholog of MED27) decreases amacrine cell number and increases rod photoreceptor cells in the retina, and eliminates dopaminergic amacrine cells, demonstrating a specific role for MED27 in retinal cell-type determination during vertebrate CNS development distinct from other Mediator subunits. |
Zebrafish forward genetic mutation analysis (m885 allele disrupts Crsp34/Med27), histological and cell-type specific marker analysis of mutant retinas |
Genetics |
Medium |
16582438
|
| 2016 |
MED27 interacts with NF-κB and with the acetyltransferase p300, and is acetylated by p300; MED27 knockdown inactivates PI3K/AKT and MAPK/ERK signaling and reduces NF-κB nuclear translocation and iNOS expression in melanoma cells. |
Co-immunoprecipitation (MED27 with NF-κB and p300), acetylation assay, siRNA knockdown with Western blot and xenograft tumor model |
Cancer letters |
Medium |
26797421
|
| 2020 |
CRSP8/MED27 binds to the IKKα promoter region (positions -257 to -143) to negatively regulate IKKα transcription; knockdown of CRSP8 upregulates IKKα, and IKKα knockdown/overexpression reverses the differentiation and EMT marker changes caused by CRSP8 manipulation in thyroid cancer cells. |
Luciferase promoter assay with deletion constructs, ChIP or promoter binding assay, siRNA knockdown, rescue experiments, xenograft mouse model |
Cell death and differentiation |
Medium |
33162555
|
| 2023 |
MED27 transcriptionally regulates KLF4 by binding to its promoter region (positions -156 to +177); KLF4 overexpression reverses the suppression of metastasis and stemness caused by MED27 silencing in breast cancer cells. |
RNA-seq to identify downstream targets, luciferase reporter assay/ChIP to map promoter binding, rescue experiments with KLF4 overexpression in vitro and in vivo |
Cancer science |
Medium |
36786527
|
| 2024 |
Cardiomyocyte-specific deletion of MED27 in mice causes embryonic lethality (constitutive) or heart failure and mortality (inducible adult deletion); MED27 loss decreases protein levels of most Mediator subunits in cardiomyocytes, indicating MED27 is required for stability of the Mediator complex; this role is independent of MED30, as MED30 overexpression fails to rescue Mediator subunit levels in MED27-deficient cardiomyocytes. |
Conditional (floxed) cardiomyocyte-specific knockout mouse (cKO and icKO), Western blot for Mediator subunit protein levels, MED30 overexpression rescue experiment, RNAseq, morphological/histological/physiological analysis |
Life sciences |
High |
39209248
|
| 2025 |
Patient-specific MED27 variants destabilize the Mediator complex and impair its chromatin occupancy and chromatin interactions; in vitro stem cell models and in vivo mouse LoF models recapitulate cerebellar atrophy and motor deficits; transcriptomic profiling identifies dysregulation of master regulatory transcription factors involved in neurogenesis and cerebellar development as downstream consequences. |
iPSC models with patient variants, Med27 LoF mouse models, molecular analyses of Mediator complex stability, chromatin occupancy assay (ChIP or related), chromatin interaction assay, single-cell spatial transcriptomics |
Advanced science (Weinheim, Baden-Wurttemberg, Germany) |
High |
41017421
|
| 2024 |
A missense variant MED27 p.Arg25His causes a significant reduction in MED27 protein expression and, by molecular dynamics modeling, is predicted to weaken the interaction between MED27 and MED14 subunits. |
Western blot of transfected cells expressing Arg25His variant, molecular dynamics structural modeling |
Heliyon |
Low |
39296199
|
| 2025 |
CRSP8/MED27 transcriptionally activates RAN expression; enhanced RAN/CRM1-mediated nuclear export induced by CRSP8 promotes cytoplasmic translocation of PPARα (formation of RAN/CRM1/PPARα heterotrimer), impairing nuclear PPARα-dependent lipophagy and fatty acid oxidation, thereby driving lipid droplet accumulation and HCC progression. |
Co-immunoprecipitation, luciferase reporter assay, live cell imaging of lipid droplets, transmission electron microscopy, targeted lipidomics, in vitro and in vivo HCC models |
Journal of experimental & clinical cancer research : CR |
Medium |
40069732
|
| 2024 |
In Saccharomyces cerevisiae, Med3 interacts with the transcription factor Stb5 (by yeast two-hybrid) to regulate expression of gnd1 and ald6 (NADPH-generating genes) under hyperosmotic stress; Med3 deletion reduces intracellular NADPH, increases ROS, and impairs bud formation under hyperosmotic conditions. |
Yeast two-hybrid (Med3–Stb5 interaction), deletion strain phenotypic analysis, NADPH and ROS measurements, gene expression analysis |
Applied and environmental microbiology |
Medium |
39082808
|