Affinage

MAT1A

S-adenosylmethionine synthase isoform type-1 · UniProt Q00266

Round 2 corrected
Length
395 aa
Mass
43.6 kDa
Annotated
2026-04-28
130 papers in source corpus 16 papers cited in narrative 16 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MAT1A encodes the liver-specific catalytic subunit of methionine adenosyltransferase (MAT), which catalyzes S-adenosylmethionine (SAM) synthesis from methionine and ATP and assembles into kinetically distinct homodimeric (MAT III) and homotetrameric (MAT I) forms with differing methionine affinities (PMID:7213623, PMID:9175157). Crystal structures reveal a compact active site with a gating loop that accommodates substrate and product conformations during catalysis, and the active-site Cys120 is a target for formaldehyde-mediated inhibition that reduces SAM production and downstream histone methylation in vivo (PMID:23425511, PMID:37917677). MAT1A expression is silenced during hepatocarcinogenesis through CpG promoter hypermethylation, CTBP1/HDAC1/HDAC2 transcriptional repression, AUF1-mediated mRNA destabilization, and targeting by miR-664/miR-485-3p/miR-495/miR-143, and its restoration raises intracellular SAM, suppresses NF-κB signaling, and promotes ferroptosis in hepatocellular carcinoma cells (PMID:22318685, PMID:37230466, PMID:20102719, PMID:23241961). Loss-of-function mutations in MAT1A cause autosomal recessive isolated persistent hypermethioninemia, with complete loss of activity leading to brain demyelination through impaired myelin phospholipid synthesis, while the dominant-negative R264H mutation poisons wild-type subunits within heterodimers (PMID:7560086, PMID:8770875, PMID:11278456).

Mechanistic history

Synthesis pass · year-by-year structured walk · 7 steps
  1. 1981 High

    Establishing that liver SAM synthesis is catalyzed by two kinetically distinct oligomeric enzyme species answered the question of why hepatic methionine metabolism shows complex cooperativity: a high-affinity tetramer (MAT I) and a lower-affinity dimer (MAT III) partition methionine flux across physiological concentration ranges.

    Evidence Partial purification, gel filtration, sedimentation, and kinetic analysis of rat liver MAT species

    PMID:7213623

    Open questions at the time
    • Subunit composition not yet linked to a specific gene
    • Regulatory mechanisms controlling interconversion between dimer and tetramer unknown
    • No structural information available
  2. 1993 High

    Cloning the human and murine MAT1A genes established that a single liver-specific gene encodes the catalytic subunit of both MAT I and MAT III, resolving the molecular identity of the liver-type SAM synthetase and revealing its tissue-restricted promoter.

    Evidence cDNA cloning, Northern blotting, Southern blotting, primer extension, and promoter-reporter assays in hepatocytes

    PMID:8314764 PMID:8393662

    Open questions at the time
    • Transcription factor network driving liver-specific expression not defined
    • No disease mutations yet identified
  3. 1996 High

    Identification of patient mutations established that MAT1A deficiency causes isolated persistent hypermethioninemia and revealed a dose-dependent genotype–phenotype correlation: complete loss of activity causes brain demyelination via impaired myelin phospholipid synthesis, while partial loss is clinically benign.

    Evidence Patient MAT1A sequencing, site-directed mutagenesis with transient expression, plasma methionine measurement, and brain MRI across multiple families

    PMID:10677294 PMID:7560086 PMID:8770875

    Open questions at the time
    • Precise threshold of residual activity that protects against neurological damage undefined
    • Mechanism linking SAM depletion to demyelination only inferred from lipid composition
  4. 2001 High

    Biochemical dissection of the R264H dominant mutation revealed a dominant-negative mechanism: the mutant subunit forms heterodimers with wild-type subunits that retain tripolyphosphatase activity but cannot synthesize SAM, explaining autosomal dominant hypermethioninemia.

    Evidence Site-directed mutagenesis, heterodimer reconstitution, kinetic assays for SAM synthesis and tripolyphosphatase activity

    PMID:11278456

    Open questions at the time
    • Structural basis for disrupted substrate binding in heterodimer not resolved
    • Whether other dominant mutations share the same mechanism unknown
  5. 2012 High

    Multi-layered silencing of MAT1A in hepatocellular carcinoma was established: promoter CpG hypermethylation and histone deacetylation suppress transcription, AUF1 destabilizes MAT1A mRNA, and miR-664/miR-485-3p/miR-495 directly repress translation, while forced MAT1A re-expression raises SAM, reduces proliferation, and downregulates NF-κB and Cyclin D1 signaling.

    Evidence Bisulfite sequencing, histone acetylation assays, RNP pulldown, miRNA knockdown/overexpression, luciferase reporters, orthotopic xenograft models

    PMID:20102719 PMID:22318685 PMID:23241961

    Open questions at the time
    • Relative contribution of each silencing layer to the MAT1A→MAT2A switch not quantified
    • Whether SAM-dependent tumor suppression operates identically across HCC subtypes untested
  6. 2013 High

    Crystal structures of human MAT1A in complex with SAM revealed the active-site architecture and gating loop dynamics underlying catalysis, providing the first structural framework for understanding isoform-specific kinetics and mutational effects.

    Evidence X-ray crystallography of human MAT1A–SAM binary complex

    PMID:23425511

    Open questions at the time
    • No substrate (methionine + ATP) ternary complex captured
    • Structural basis for tetramer vs. dimer assembly not resolved at atomic level
  7. 2023 High

    Two new regulatory axes were identified: formaldehyde inhibits MAT1A by reacting with the hyperreactive Cys120 in the active site, coupling one-carbon metabolism to epigenetic output, while a CTBP1/HDAC1/HDAC2 repressor complex directly silences MAT1A transcription in HCC, with MAT1A re-expression promoting ferroptosis and enhancing anti-tumor CD8+ T-cell immunity.

    Evidence Chemoproteomics with cysteine profiling, genetic FA-overload mouse model, histone methylation analysis; Co-IP, ChIP, xenograft models, ferroptosis assays, CD8+ T-cell cytotoxicity assays

    PMID:37230466 PMID:37917677

    Open questions at the time
    • Whether Cys120 modification is reversible in vivo and the identity of the repair pathway unknown
    • How SAM mechanistically triggers ferroptosis (lipid peroxidation substrate or iron regulation) not delineated
    • Interaction between formaldehyde sensing and HCC-associated MAT1A silencing unexplored

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis for MAT I tetramer versus MAT III dimer equilibrium, the identity of factors that govern oligomeric interconversion in vivo, whether formaldehyde-Cys120 modification is enzymatically reversible, and the precise SAM-dependent mechanism by which MAT1A promotes ferroptosis.
  • No ternary substrate complex structure
  • Dimer-tetramer interconversion mechanism unknown
  • Ferroptosis pathway downstream of SAM not defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 5 GO:0140657 ATP-dependent activity 3
Localization
GO:0005829 cytosol 2
Pathway
R-HSA-1430728 Metabolism 5 R-HSA-1643685 Disease 3 R-HSA-5357801 Programmed Cell Death 2
Partners
AUF1CTBP1HDAC1HDAC2
Complex memberships
MAT I (homotetramer)MAT III (homodimer)

Evidence

Reading pass · 16 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1993 MAT1A (liver S-adenosylmethionine synthetase) is encoded by a single-copy gene expressed exclusively in the liver, producing a single mRNA species encoding a 395-amino-acid protein with a calculated molecular mass of ~43.6 kDa. The gene structure and tissue-specific expression were characterized by Southern, Northern, and primer extension analyses. cDNA cloning, Southern blotting, Northern blotting, primer extension The Biochemical Journal High 8393662
1993 The murine MAT1A ortholog (adult liver S-adenosylmethionine synthetase) spans ~18 kb with nine exons, contains a TATA box at -28 to -23 bp, and the -365 to -2 bp region drives liver-specific expression. The enzyme shares 96% amino acid identity with the human enzyme and contains the conserved ATP-binding motif Gly-X-Gly-X-X-Gly. cDNA/genomic cloning, transient expression assays in hepatocytes, primer extension, RACE The Journal of Biological Chemistry High 8314764
1981 Rat liver contains two species of S-adenosylmethionine synthetase (MAT I/III): synthetase alpha (apparent MW ~210 kDa, 9S, tetramer) exhibits negative cooperativity with low S0.5 for methionine (~17 µM) and ATP (~0.5 mM), while synthetase beta (apparent MW ~160 kDa, 5.5S, dimer) shows positive cooperativity with higher S0.5 values. Both require Mg²⁺ and K⁺ and are inhibited by p-chloromercuribenzoate, indicating essential thiols. Partial purification (~200-1000-fold), gel filtration, sedimentation analysis, kinetic studies Biochemistry High 7213623
1997 Mammalian liver MAT (encoded by MAT1A) exists as two oligomeric forms: a homodimer (MAT III) and a homotetramer (MAT I) of the same catalytic subunit. Both isoenzymes are inhibited in human liver cirrhosis, explaining abnormal methionine metabolism in cirrhotic patients. Biochemical fractionation, molecular characterization, clinical liver biopsy analysis Pharmacology & Therapeutics High 9175157
1995 Mutations in the MAT1A gene cause partial inactivation of methionine adenosyltransferase activity, leading to isolated persistent hypermethioninemia. Site-directed mutagenesis of identified patient mutations and transient expression assays established that these mutations reduce but do not abolish MAT catalytic activity. Gene characterization, site-directed mutagenesis, transient expression assays The Journal of Clinical Investigation High 7560086
1996 Complete loss of MAT1A activity (homozygous truncating mutations) causes brain demyelination, while missense mutations that partially retain activity do not. Mutations in the MATA1 gene that alter the reading frame produce truncated, enzymatically inactive MATα1 subunits, and the resulting loss of S-adenosylmethionine impairs synthesis of myelin phospholipids (phosphatidylcholine and sphingomyelin). Patient MATA1 gene sequencing, mutation characterization, correlation of genotype with neurological phenotype The Journal of Clinical Investigation High 8770875
2000 Six novel MAT1A mutations were identified; compound heterozygotes and homozygotes for truncating/severely inactivating mutations develop very high plasma methionine (1226-1870 µM) and manifest brain gray matter abnormalities or demyelination, while patients with inactivating missense mutations or the dominant R264H mutation show lower methionine (105-467 µM) and are clinically unaffected, establishing a genotype-phenotype correlation. MAT1A sequencing in hypermethioninemic patients, plasma methionine measurement, brain MRI American Journal of Human Genetics High 10677294
2001 The R264H (R265H in rat) mutation produces a monomeric MAT with only 0.37% of AdoMet synthetic activity but retains tripolyphosphatase activity similar to wild-type. The R265H mutant subunit associates with wild-type subunit to form a heterodimer that cannot synthesize AdoMet; tripolyphosphatase activity is maintained in this hybrid but is not stimulated by methionine and ATP, indicating substrate binding is disrupted. This explains dominant inheritance of hypermethioninemia by dominant-negative subunit poisoning. Site-directed mutagenesis, in vivo expression, kinetic assays, activity measurements The Journal of Biological Chemistry High 11278456
2010 AUF1 binds the 3'-UTR of MAT1A mRNA and destabilizes it, while HuR stabilizes MAT2A mRNA. During hepatic de-differentiation and in HCC, increased AUF1 and HuR expression drives the MAT1A→MAT2A switch. S-adenosylmethionine treatment shifts the balance toward methyl-HuR (an inhibitor of MAT2A mRNA stability), restoring MAT1A expression. Both RNA-binding proteins regulate the MAT switch during fetal liver development and HCC progression. RNA-binding protein pulldown, mRNA stability assays, immunofluorescence, RNP complex analysis, in vitro and in vivo studies Gastroenterology High 20102719
2012 Three microRNAs — miR-664, miR-485-3p, and miR-495 — are upregulated in HCC and directly repress MAT1A expression. Knockdown of each miRNA individually or in combination induces MAT1A expression, reduces HCC cell growth, and increases apoptosis. Maintaining MAT1A expression significantly blocked the antitumorigenic effect of miRNA knockdown. Mechanistically, miRNA-mediated MAT1A repression reduces global CpG methylation and increases LIN28B expression. miRNA knockdown, stable overexpression, orthotopic liver cancer xenograft models, luciferase reporter assays The Journal of Clinical Investigation High 23241961
2012 MAT1A promoter undergoes CpG hypermethylation and histone H4 deacetylation in fast-growing HCC, while MAT2A promoter shows hypomethylation and H4 acetylation, driving the MAT1A:MAT2A switch. AUF1 protein destabilizes MAT1A mRNA and HuR stabilizes MAT2A mRNA in HCC. Forced MAT1A overexpression in HCC cells raises SAM levels, decreases cell proliferation, increases apoptosis, downregulates Cyclin D1, E2F1, IKK, NF-κB, and antiapoptotic BCL2/XIAP, and upregulates proapoptotic BAX/BAK. Promoter methylation analysis (bisulfite sequencing), histone acetylation assays, RNP complex analysis, forced overexpression with proliferation and apoptosis readouts Hepatology High 22318685
2013 Crystal structures of human MAT1A and MAT2A in binary complexes with their product SAM were determined, revealing a compact active site with a gating loop that accommodates different substrate/product conformations during catalysis. Comparison with E. coli and rat structures shows the structural basis for catalysis. MAT2B has an SDR core with an NADP/H cofactor specificity and the SDR catalytic triad (YxxxKS). X-ray crystallography, ligand-bound complex structures The Biochemical Journal High 23425511
2023 Formaldehyde (FA) inhibits MAT1A by reacting with a hyperreactive cysteine residue (Cys120) in the active site, reducing SAM production in a MAT-isoform-specific manner. A genetic mouse model of chronic FA overload showed decreased SAM and reduced methylation on selected histones and genes. Epigenetic and transcriptional regulation of Mat1a serve as compensatory mechanisms for FA-dependent SAM depletion, revealing a biochemical feedback cycle. Chemoproteomics (cysteine reactivity profiling), in vitro MAT1A activity assays, genetic mouse model, histone methylation analysis Science High 37917677
2023 A CTBP1/HDAC1/HDAC2 transcriptional repressor complex directly suppresses MAT1A transcription in HCC cells. Silencing HDAC1 or HDAC2, or overexpressing MAT1A, inhibited cancer cell malignancy. MAT1A overexpression increased intracellular SAM levels, which promoted ferroptosis of HCC cells and enhanced CD8+ T-cell cytotoxicity and IFN-γ production. In vivo, MAT1A overexpression suppressed CTBP1-induced xenograft tumor growth, and ferroptosis inhibitor ferrostatin-1 blocked these tumor-suppressive effects. Co-immunoprecipitation, chromatin immunoprecipitation, siRNA knockdown, MAT1A overexpression, xenograft mouse models, ferroptosis assays, CD8+ T-cell cytotoxicity assays Laboratory Investigation High 37230466
2022 miR-143 targets MAT1a mRNA in liver, and betaine supplementation downregulates miR-143 expression, which in turn increases SAM levels by de-repressing MAT1a. This regulatory axis controls hepatic lipogenesis and gluconeogenesis, with miR-143 KO promoting lipolysis and glucose utilization through repression of GPNMB and targeting MAPK11. miR-143 knockout mouse model, betaine supplementation experiments, luciferase reporter assays for MAT1a 3'-UTR targeting, metabolic phenotyping Journal of Agricultural and Food Chemistry Medium 35734958
2019 MAT1A overexpression in bladder cancer cells (5637 line) increased tolerance to gemcitabine and stalled cell proliferation, suggesting MAT1A upregulation allows cancer cells to persist in a quiescent state to evade chemotherapy. MAT1A was identified as significantly upregulated in patient-derived xenograft tumors following cisplatin/gemcitabine treatment. PDX transcriptomic profiling (RNA-seq), MAT1A overexpression in cell lines, proliferation and drug tolerance assays International Journal of Molecular Sciences Medium 31600961

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
1997 S-adenosylmethionine synthesis: molecular mechanisms and clinical implications. Pharmacology & therapeutics 386 9175157
2020 CircRNA-SORE mediates sorafenib resistance in hepatocellular carcinoma by stabilizing YBX1. Signal transduction and targeted therapy 366 33361760
2012 Mating-type genes and MAT switching in Saccharomyces cerevisiae. Genetics 301 22555442
2006 Unexpected diversity and complexity of the Guerrero Negro hypersaline microbial mat. Applied and environmental microbiology 286 16672518
1995 Crystal structure of the MATa1/MAT alpha 2 homeodomain heterodimer bound to DNA. Science (New York, N.Y.) 234 7569974
1991 Association of demyelination with deficiency of cerebrospinal-fluid S-adenosylmethionine in inborn errors of methyl-transfer pathway. Lancet (London, England) 202 1683972
2011 Radical-mediated enzymatic methylation: a tale of two SAMS. Accounts of chemical research 184 22097883
1998 Degradation signal masking by heterodimerization of MATalpha2 and MATa1 blocks their mutual destruction by the ubiquitin-proteasome pathway. Cell 170 9695950
2004 Evolution of the MAT locus and its Ho endonuclease in yeast species. Proceedings of the National Academy of Sciences of the United States of America 165 14745027
2009 Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. American journal of human genetics 164 19913121
2000 Metaphase to anaphase (mat) transition-defective mutants in Caenorhabditis elegans. The Journal of cell biology 142 11134076
1995 Mat-8, a novel phospholemman-like protein expressed in human breast tumors, induces a chloride conductance in Xenopus oocytes. The Journal of biological chemistry 138 7836447
2005 A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease. American journal of human genetics 137 16385451
2012 MicroRNAs regulate methionine adenosyltransferase 1A expression in hepatocellular carcinoma. The Journal of clinical investigation 130 23241961
1984 The yeast MATa1 gene contains two introns. The EMBO journal 124 6329735
1981 Multiple species of mammalian S-adenosylmethionine synthetase. Partial purification and characterization. Biochemistry 123 7213623
2015 Investigation of Bovine Serum Albumin (BSA) Attachment onto Self-Assembled Monolayers (SAMs) Using Combinatorial Quartz Crystal Microbalance with Dissipation (QCM-D) and Spectroscopic Ellipsometry (SE). PloS one 121 26505481
1992 IME4, a gene that mediates MAT and nutritional control of meiosis in Saccharomyces cerevisiae. Molecular and cellular biology 120 1545790
2010 HuR/methyl-HuR and AUF1 regulate the MAT expressed during liver proliferation, differentiation, and carcinogenesis. Gastroenterology 114 20102719
2022 EZH2 depletion potentiates MYC degradation inhibiting neuroblastoma and small cell carcinoma tumor formation. Nature communications 99 35013218
2009 Bacterial, archaeal and eukaryotic diversity of smooth and pustular microbial mat communities in the hypersaline lagoon of Shark Bay. Geobiology 94 19200148
1996 Demyelination of the brain is associated with methionine adenosyltransferase I/III deficiency. The Journal of clinical investigation 91 8770875
2020 Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains. Cell reports 79 32814053
2012 Role of transcriptional and posttranscriptional regulation of methionine adenosyltransferases in liver cancer progression. Hepatology (Baltimore, Md.) 79 22318685
2011 Knockdown of SAMS genes encoding S-adenosyl-l-methionine synthetases causes methylation alterations of DNAs and histones and leads to late flowering in rice. Journal of plant physiology 77 21757254
2010 Human variation in alcohol response is influenced by variation in neuronal signaling genes. Alcoholism, clinical and experimental research 74 20201926
1993 Characterization of a full-length cDNA encoding human liver S-adenosylmethionine synthetase: tissue-specific gene expression and mRNA levels in hepatopathies. The Biochemical journal 74 8393662
2017 A viral protein promotes host SAMS1 activity and ethylene production for the benefit of virus infection. eLife 73 28994391
2000 Cloning, expression, and functional characterization of the beta regulatory subunit of human methionine adenosyltransferase (MAT II). The Journal of biological chemistry 69 10644686
2010 Isolation and characterization of MAT genes in the symbiotic ascomycete Tuber melanosporum. The New phytologist 66 20961294
2017 A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells. Cell reports 64 28700943
2016 Surface Analysis of Gold Nanoparticles Functionalized with Thiol-Modified Glucose SAMs for Biosensor Applications. Frontiers in chemistry 63 26973830
2000 Methionine adenosyltransferase I/III deficiency: novel mutations and clinical variations. American journal of human genetics 63 10677294
1993 Cloning and expression of murine S-adenosylmethionine synthetase. The Journal of biological chemistry 63 8314764
2009 An association study of 45 folate-related genes in spina bifida: Involvement of cubilin (CUBN) and tRNA aspartic acid methyltransferase 1 (TRDMT1). Birth defects research. Part A, Clinical and molecular teratology 61 19161160
2008 Diversity and stratification of archaea in a hypersaline microbial mat. Applied and environmental microbiology 61 19114531
1998 Characterization of mat A-2, mat A-3 and deltamatA mating-type mutants of Neurospora crassa. Genetics 60 9539425
2015 P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design. Bioinformatics (Oxford, England) 59 26382195
2006 Immobilization of Protein A on SAMs for the elaboration of immunosensors. Colloids and surfaces. B, Biointerfaces 59 17056235
2009 Organization and evolutionary trajectory of the mating type (MAT) locus in dermatophyte and dimorphic fungal pathogens. Eukaryotic cell 58 19880755
2023 Formaldehyde regulates S-adenosylmethionine biosynthesis and one-carbon metabolism. Science (New York, N.Y.) 57 37917677
2013 Insight into S-adenosylmethionine biosynthesis from the crystal structures of the human methionine adenosyltransferase catalytic and regulatory subunits. The Biochemical journal 57 23425511
1986 Identification of the DNA sequences controlling the expression of the MAT alpha locus of yeast. Proceedings of the National Academy of Sciences of the United States of America 56 3517864
2015 Acetylation of MAT IIα represses tumour cell growth and is decreased in human hepatocellular cancer. Nature communications 55 25925782
1995 Molecular mechanisms of an inborn error of methionine pathway. Methionine adenosyltransferase deficiency. The Journal of clinical investigation 54 7560086
2022 Dietary folate drives methionine metabolism to promote cancer development by stabilizing MAT IIA. Signal transduction and targeted therapy 51 35729157
1991 The role of RAP1 in the regulation of the MAT alpha locus. Molecular and cellular biology 51 1990267
2022 Coaxially Fabricated Dual-Drug Loading Electrospinning Fibrous Mat with Programmed Releasing Behavior to Boost Vascularized Bone Regeneration. Advanced healthcare materials 50 35668705
2007 Mat formation in Saccharomyces cerevisiae requires nutrient and pH gradients. Eukaryotic cell 50 17951523
2014 Immune regulation by intralymphatic immunotherapy with modular allergen translocation MAT vaccine. Allergy 45 24934402
1997 Energetics of folding and DNA binding of the MAT alpha 2 homeodomain. Biochemistry 44 9012668
1994 Heterodimerization of the yeast MATa1 and MAT alpha 2 proteins is mediated by two leucine zipper-like coiled-coil motifs. The EMBO journal 43 8137824
2012 The response regulator RcsB activates expression of Mat fimbriae in meningitic Escherichia coli. Journal of bacteriology 41 22522901
2014 Bi-layer scaffold of chitosan/PCL-nanofibrous mat and PLLA-microporous disc for skin tissue engineering. Journal of biomedical nanotechnology 40 24749404
2011 Contribution of sams-1 and pmt-1 to lipid homoeostasis in adult Caenorhabditis elegans. Journal of biochemistry 40 21389045
1998 Crystal structure of the MATa1/MATalpha2 homeodomain heterodimer in complex with DNA containing an A-tract. Nucleic acids research 37 9838003
2003 Insights into binding cooperativity of MATa1/MATalpha2 from the crystal structure of a MATa1 homeodomain-maltose binding protein chimera. Protein science : a publication of the Protein Society 36 12538894
2009 Spatial and temporal variability in a stratified hypersaline microbial mat community. FEMS microbiology ecology 35 19175677
1999 Thiomicrospira chilensis sp. nov., a mesophilic obligately chemolithoautotrophic sulfuroxidizing bacterium isolated from a Thioploca mat. International journal of systematic bacteriology 35 10319513
2002 maT--a clade of transposons intermediate between mariner and Tc1. Molecular biology and evolution 34 12446802
2013 MAT gene idiomorphs suggest a heterothallic sexual cycle in a predominantly asexual and important pine pathogen. Fungal genetics and biology : FG & B 28 24220137
2006 Modulating cell adhesion and spreading by control of FnIII7-10 orientation on charged self-assembled monolayers (SAMs) of alkanethiolates. Journal of biomedical materials research. Part A 28 16514600
2011 Quality control of MATa1 splicing and exon skipping by nuclear RNA degradation. Nucleic acids research 27 22021379
1981 The mat-1 gene in Chlamydomonas regulates DNA methylation during gametogenesis. Cell 27 6263491
2019 Comparison of tissues (heart vs. brain) and serological tests (MAT, ELISA and IFAT) for detection of Toxoplasma gondii in naturally infected wolverines (Gulo gulo) from the Yukon, Canada. Food and waterborne parasitology 26 32095617
2022 SAMS-1 coordinates HLH-30/TFEB and PHA-4/FOXA activities through histone methylation to mediate dietary restriction-induced autophagy and longevity. Autophagy 25 35503435
2001 Biochemical basis for the dominant inheritance of hypermethioninemia associated with the R264H mutation of the MAT1A gene. A monomeric methionine adenosyltransferase with tripolyphosphatase activity. The Journal of biological chemistry 25 11278456
1999 A novel role for the mating type (MAT) locus in the maintenance of cell wall integrity in Saccharomyces cerevisiae. Molecular & general genetics : MGG 25 10394905
1994 Brain ATP:L-methionine S-adenosyltransferase (MAT), S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH): regional distribution and age-related changes. European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology 25 7894257
2020 Combined PCR and MAT improves the early diagnosis of the biphasic illness leptospirosis. PloS one 24 32915919
2014 Unraveling the stratification of an iron-oxidizing microbial mat by metatranscriptomics. PloS one 24 25033299
1991 MAT alpha 1 can mediate gene activation by a-mating factor. Genes & development 24 1916267
2017 Comparison of the active and resident community of a coastal microbial mat. Scientific reports 23 28592823
2023 Protein translocation in mitochondria: Sorting out the Toms, Tims, Pams, Sams and Mia. FEBS letters 22 37021373
2020 Sphinganine-Analog Mycotoxins (SAMs): Chemical Structures, Bioactivities, and Genetic Controls. Journal of fungi (Basel, Switzerland) 22 33255427
2016 Fabrication, characterization and biomedical application of two-nozzle electrospun polycaprolactone/zein-calcium lactate composite nonwoven mat. Journal of the mechanical behavior of biomedical materials 22 26919567
2013 Phylogenetic group-associated differences in regulation of the common colonization factor Mat fimbria in Escherichia coli. Molecular microbiology 22 23347101
2004 Chemopreventive N-(4-hydroxyphenyl)retinamide (fenretinide) targets deregulated NF-{kappa}B and Mat1A genes in the early stages of rat liver carcinogenesis. Carcinogenesis 21 15498786
2012 Regulation of mat responses by a differentiation MAPK pathway in Saccharomyces cerevisiae. PloS one 20 22496730
2024 Peptide-based self-assembled monolayers (SAMs): what peptides can do for SAMs and vice versa. Chemical Society reviews 19 38456490
2022 miR-143-Mediated Responses to Betaine Supplement Repress Lipogenesis and Hepatic Gluconeogenesis by Targeting MAT1a and MAPK11. Journal of agricultural and food chemistry 18 35734958
2018 Functional and Expression Analyses of the Pneumocystis MAT Genes Suggest Obligate Sexuality through Primary Homothallism within Host Lungs. mBio 18 29463658
1991 AAR2, a gene for splicing pre-mRNA of the MATa1 cistron in cell type control of Saccharomyces cerevisiae. Molecular and cellular biology 18 1922071
2018 Going with the Flo: The Role of Flo11-Dependent and Independent Interactions in Yeast Mat Formation. Journal of fungi (Basel, Switzerland) 17 30544497
2017 Genomic Reconstruction of Carbohydrate Utilization Capacities in Microbial-Mat Derived Consortia. Frontiers in microbiology 17 28751880
2016 Genetic Variants in CD44 and MAT1A Confer Susceptibility to Acute Skin Reaction in Breast Cancer Patients Undergoing Radiation Therapy. International journal of radiation oncology, biology, physics 17 27816361
2012 Human liver methionine cycle: MAT1A and GNMT gene resequencing, functional genomics, and hepatic genotype-phenotype correlation. Drug metabolism and disposition: the biological fate of chemicals 17 22807109
2012 Cell attachment to hydrogel-electrospun fiber mat composite materials. Journal of functional biomaterials 17 24955629
2004 Lissencephaly with der(17)t(17;20)(p13.3;p12.2)mat. American journal of medical genetics. Part A 17 14708103
1979 Trisomy 8p due to the 3:1 segregation of the balanced translocation t(8;15)mat. Human genetics 17 437777
2022 Protein and polysaccharide-based asymmetric mat with tuned bilayer configuration for enhanced wound healing efficiency. Carbohydrate polymers 16 35725208
2019 Seasonal development of a coastal microbial mat. Scientific reports 16 31227767
2005 Methionine adenosyltransferase (MAT) I/III deficiency with concurrent hyperhomocysteinaemia: two novel cases. Journal of inherited metabolic disease 16 16435220
2023 Antibacterial and antioxidant double-layered nanofibrous mat promotes wound healing in diabetic rats. Scientific reports 15 36823173
2023 MAT1A Suppression by the CTBP1/HDAC1/HDAC2 Transcriptional Complex Induces Immune Escape and Reduces Ferroptosis in Hepatocellular Carcinoma. Laboratory investigation; a journal of technical methods and pathology 15 37230466
2020 Investigating the Smuts: Common Cues, Signaling Pathways, and the Role of MAT in Dimorphic Switching and Pathogenesis. Journal of fungi (Basel, Switzerland) 15 33339287
2016 Fabrication of Gelatin/PCL Electrospun Fiber Mat with Bone Powder and the Study of Its Biocompatibility. Journal of functional biomaterials 15 26959071
2014 Thirteen Patients with MAT1A Mutations Detected Through Newborn Screening: 13 Years' Experience. JIMD reports 15 24445979
2011 Vacuolar protein sorting genes regulate mat formation in Saccharomyces cerevisiae by Flo11p-dependent and -independent mechanisms. Eukaryotic cell 14 21908597
2023 Electrospun nanofiber composite mat based on ulvan for wound dressing applications. International journal of biological macromolecules 13 37659492
2022 Magnolol Hybrid Nanofibrous Mat with Antibacterial, Anti-Inflammatory, and Microvascularized Properties for Wound Treatment. Biomacromolecules 13 35067057
2019 Methionine Adenosyltransferase 1a (MAT1A) Enhances Cell Survival During Chemotherapy Treatment and is Associated with Drug Resistance in Bladder Cancer PDX Mice. International journal of molecular sciences 13 31600961
2018 Engineering of Neuron Growth and Enhancing Cell-Chip Communication via Mixed SAMs. ACS applied materials & interfaces 13 29763286
2004 Repression of the yeast HO gene by the MATalpha2 and MATa1 homeodomain proteins. Nucleic acids research 13 15598821
2020 Mast Cell Activation Test (MAT). Methods in molecular biology (Clifton, N.J.) 12 32766980
2019 SAHH and SAMS form a methyl donor complex with CCoAOMT7 for methylation of phenolic compounds. Biochemical and biophysical research communications 12 31582217
2018 Low-Fouling Characteristics of Ultrathin Zwitterionic Cysteine SAMs. Langmuir : the ACS journal of surfaces and colloids 12 30056710
2011 A novel globular protein electrospun fiber mat with the addition of polysilsesquioxane. International journal of biological macromolecules 12 21664927
2018 Dye-Sensitized Solar Cells with Electrospun Nanofiber Mat-Based Counter Electrodes. Materials (Basel, Switzerland) 11 30181451
2017 Formation of upd(7)mat by trisomic rescue: SNP array typing provides new insights in chromosomal nondisjunction. Molecular cytogenetics 11 28770003
2017 Methanogenic and Sulfate-Reducing Activities in a Hypersaline Microbial Mat and Associated Microbial Diversity. Microbial ecology 11 29116347
2016 Microbial Mat Compositional and Functional Sensitivity to Environmental Disturbance. Frontiers in microbiology 11 27799927
2010 Synthesis of benzaldehyde-functionalized glycans: a novel approach towards glyco-SAMs as a tool for surface plasmon resonance studies. Chemistry (Weinheim an der Bergstrasse, Germany) 11 20432412
2003 Characterization of sams genes of Amoeba proteus and the endosymbiotic X-bacteria. The Journal of eukaryotic microbiology 11 12674481
2000 Cooperative ordering in homeodomain-DNA recognition: solution structure and dynamics of the MATa1 homeodomain. Biochemistry 11 10955992
2023 MAT as a promising therapeutic strategy against triple-negative breast cancer via inhibiting PI3K/AKT pathway. Scientific reports 10 37524857
2022 Deep phenotyping: symptom annotation made simple with SAMS. Nucleic acids research 10 35524573
2013 Effects of hydrophobicity and mat thickness on release from hydrogel-electrospun fiber mat composites. Journal of biomaterials science. Polymer edition 10 23905840
2009 Chemical and biological characterization of thiol SAMs for neuronal cell attachment. Langmuir : the ACS journal of surfaces and colloids 10 19281272
1988 Splicing and spliceosome formation of the yeast MATa1 transcript require a minimum distance from the 5' splice site to the internal branch acceptor site. Nucleic acids research 10 3054807