Affinage

MAPRE3

Microtubule-associated protein RP/EB family member 3 · UniProt Q9UPY8

Length
281 aa
Mass
32.0 kDa
Annotated
2026-06-10
38 papers in source corpus 26 papers cited in narrative 26 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MAPRE3/EB3 is a microtubule plus-end tracking protein (+TIP) that autonomously marks growing microtubule ends and acts as a context-specific scaffold linking microtubule dynamics to actin, adhesion, calcium signaling, and cellular morphogenesis (PMID:12684451, PMID:16148041). It directly binds CLIP-170/CLIP-115 through their C-terminal tyrosine residues and controls their dissociation kinetics from microtubule tips (PMID:16148041), targets the catastrophe factor MCAK to growing ends (PMID:20850319), and undergoes liquid-liquid phase separation that recruits tubulin and nucleates polymerization more efficiently than EB1 (PMID:41135671). EB3 bridges the microtubule and F-actin networks by binding drebrin on F-actin, a coupling required for growth cone formation, neurite extension, and apical epithelial elongation (PMID:18806788, PMID:22275434, PMID:34478582), and it associates with structural scaffolds including ankyrin G at the axon initial segment, myomegalin/CAMSAP2 tethers at Golgi membranes, and BPAG1 (PMID:21551097, PMID:28814570, PMID:25244344). Through an SxIP/TxIP motif EB3 binds IP3R3 and STIM2 to amplify ER calcium release: in endothelial cells the EB3-IP3R3 interaction drives thrombin- and inflammation-induced Ca2+ flux, junction disassembly, and vascular permeability, which a peptide targeting the EB3-IP3R3 interface (CIPRI) reverses in vivo (PMID:26119739, PMID:37290041, PMID:29247211). EB3 abundance and activity are tightly regulated: Aurora A/B phosphorylation at Ser-176 stabilizes EB3 during mitosis by disrupting SIAH-1-mediated ubiquitination and degradation to support cell cycle progression and cytokinesis (PMID:19696028, PMID:23712260), calcineurin-driven Ser-162 phosphorylation destabilizes the dimer to permit adherens junction assembly (PMID:23159740), and EZH2-mediated H3K27 trimethylation epigenetically represses MAPRE3, with re-expression inducing cell cycle arrest and apoptosis in ovarian cancer (PMID:38199479). EB3 is also selectively required for myoblast fusion and primary cilium assembly (PMID:17658256, PMID:21768326).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 2000 Medium

    Established EB3 as a microtubule-associated EB1-family protein with a defined binding partner, framing it as a candidate microtubule regulator in the CNS.

    Evidence Yeast two-hybrid and confocal co-localization identifying binding to APCL/APC2

    PMID:10644998

    Open questions at the time
    • No direct demonstration of plus-end tracking
    • Functional consequence of APCL binding not established
  2. 2003 High

    Showed EB3 autonomously tracks growing microtubule plus-ends in living neurons, reporting microtubule polarity and growth in dendrites.

    Evidence Live-cell EB3-GFP imaging with quantitative directional analysis in cultured neurons

    PMID:12684451

    Open questions at the time
    • Molecular basis of end recognition not defined
    • Does not address how EB3 differs functionally from EB1
  3. 2005 High

    Defined EB3 as a recruitment hub that controls partner residence at microtubule tips, answering how +TIP networks are organized.

    Evidence Direct binding assays plus RNAi depletion and rescue measuring CLIP-170/CLIP-115 dissociation kinetics

    PMID:16148041

    Open questions at the time
    • Stoichiometry of EB3-CLIP complexes unresolved
    • EB1 vs EB3 differential roles not fully separated
  4. 2007 High

    Revealed cell-type-specific, non-redundant EB3 functions in myoblast fusion and beta-catenin signaling, distinguishing it from EB1.

    Evidence RNAi knockdown, EB1/EB3 chimeras, and C2C12 differentiation; separate Y2H/pull-down with DDA3 plus reporter assays

    PMID:17310996 PMID:17658256

    Open questions at the time
    • Calponin-domain residues driving fusion not mechanistically explained
    • DDA3 cooperation only Medium confidence
  5. 2008 High

    Identified direct EB3-drebrin binding as a molecular coupler between dynamic microtubule tips and F-actin, explaining microtubule guidance in growth cones.

    Evidence In vitro direct binding, co-localization, and dominant-negative neurite outgrowth assays

    PMID:18806788

    Open questions at the time
    • Regulation of the EB3-drebrin interaction not defined
  6. 2009 High

    Established that EB3 protein levels are cell-cycle regulated through a kinase/ubiquitin-ligase switch, linking +TIP abundance to mitotic progression.

    Evidence In vitro Aurora-A/B kinase assays, co-IP of SIAH-1 complex disruption, RNAi and proteasome inhibition

    PMID:19696028

    Open questions at the time
    • Structural basis of Ser-176 phospho-control of SIAH-1 binding unresolved
  7. 2010 High

    Demonstrated with purified components that EB3 both rescues microtubule growth against a catastrophe factor and targets that factor to ends, defining a dual regulatory activity.

    Evidence In vitro reconstitution of microtubule dynamics with TIRF microscopy and MCAK

    PMID:20850319

    Open questions at the time
    • Physiological context of EB3-MCAK regulation in cells not addressed
  8. 2011 High

    Placed EB3 at specialized cellular landmarks—the axon initial segment, primary cilia base, and dendritic branch points—through scaffold interactions controlling its localization and comet lifetime.

    Evidence Direct interaction/co-IP with ankyrin G and PSD-95, GST pull-down/MS at the cilium base, knockdown and EM phenotypes

    PMID:21248129 PMID:21551097 PMID:21768326

    Open questions at the time
    • PSD-95 interaction only Medium confidence
    • How EB3 is stabilized at the AIS mechanistically unresolved
  9. 2012 High

    Showed EB3 dimer stability is a regulated node coupling calcium/calcineurin signaling and actin-MT crosstalk to junction and epithelial morphogenesis.

    Evidence Ser-162 phospho-mutagenesis with Ca2+ imaging and permeability assays; co-IP of drebrin/myosin IIB/spectrin complex with knockdown

    PMID:22275434 PMID:23159740

    Open questions at the time
    • Epithelial drebrin-EB3 complex only Medium confidence
    • Kinase acting downstream of calcineurin on Ser-162 not identified
  10. 2013 High

    Defined a distinct EB3 role in late mitosis—midbody microtubule stability, focal adhesion control, and cytokinesis—via Aurora-B phosphorylation at Ser-176.

    Evidence Phospho-mutagenesis, live-cell imaging, and RNAi in dividing cells

    PMID:23712260

    Open questions at the time
    • How the same Ser-176 site governs both stability and cortical MT growth not integrated
  11. 2015 High

    Established EB3 as a direct amplifier of IP3R3 calcium signaling in endothelium, providing in vivo evidence for its role in vascular permeability.

    Evidence Motif mutagenesis, siRNA, endothelial conditional knockout mouse, Ca2+ imaging, permeability assays

    PMID:26119739

    Open questions at the time
    • Mechanism by which EB3 promotes IP3R3 clustering not structurally defined
  12. 2017 High

    Extended EB3 SxIP-motif calcium coupling to neurons, linking STIM2-EB3 to dendritic spine maintenance and Alzheimer's-model spine rescue.

    Evidence Co-IP, SxIP motif mutagenesis, knockdown/overexpression rescue, spine morphometry; co-IP of Golgi myomegalin tether with CRISPR knockout

    PMID:28814570 PMID:29247211

    Open questions at the time
    • STIM2-EB3 finding only Medium confidence
    • Selectivity of EB3 for distinct SxIP partners in vivo unresolved
  13. 2018 Low

    Suggested EB3 forms a phosphoregulated complex with the kinase ITPKA that dynamically cycles during synaptic plasticity.

    Evidence Co-IP with phospho-mutant and chemical LTP induction

    PMID:30466786

    Open questions at the time
    • Single co-IP without reciprocal or in vitro binding validation
    • Functional consequence of complex cycling not established
  14. 2021 High

    Resolved EB3 vs EB1 spatial specialization at growth cone tips, explaining why EB3 specifically drives neuritogenesis through the drebrin/F-actin zippering pathway.

    Evidence CRISPR editing, pharmacological MT perturbation, and live imaging of plus-end position

    PMID:34478582

    Open questions at the time
    • Molecular basis of EB3's more proximal tip localization not defined
  15. 2023 High

    Identified EB3 as a regulator of the labile microtubule pool via the severing enzyme fidgetin and developed a peptide therapeutic against the EB3-IP3R3 interface for lung injury.

    Evidence RNAi/epistasis with fidgetin in an SCI model; CIPRI peptide competition with in vitro and in vivo endotoxemia/sepsis validation

    PMID:37290041 PMID:37449637

    Open questions at the time
    • Fidgetin study only Medium confidence
    • Whether CIPRI affects EB3's other SxIP interactions unaddressed
  16. 2024 High

    Defined biophysical and transcriptional layers of EB3 control—its higher LLPS propensity and tubulin-nucleating capacity versus EB1, GTP-cap-independent comet behavior, and EZH2/H3K27me3 epigenetic repression with tumor-suppressive re-expression.

    Evidence In vitro LLPS/FRAP/polymerization assays, reconstitution with microfabricated barriers and modeling, and ChIP/knockdown/xenograft in ovarian cancer

    PMID:38199479 PMID:39604262 PMID:41135671

    Open questions at the time
    • EZH2/ovarian cancer link only Medium confidence
    • Comet-modeling study only Medium confidence
    • How LLPS relates to physiological +TIP recruitment in cells not established

Open questions

Synthesis pass · forward-looking unresolved questions
  • How EB3's many context-specific partner interactions are coordinated and prioritized within a single cell—and how its phase-separation behavior governs partner recruitment in vivo—remains unresolved.
  • No integrated model selecting among CLIP-170, drebrin, IP3R3, STIM2, and Golgi/AIS scaffold engagements
  • Structural basis of SxIP-partner discrimination not solved
  • In vivo relevance of LLPS to plus-end function untested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 4 GO:0060090 molecular adaptor activity 4 GO:0098772 molecular function regulator activity 3
Localization
GO:0005856 cytoskeleton 3 GO:0005794 Golgi apparatus 2 GO:0005815 microtubule organizing center 1 GO:0005829 cytosol 1 GO:0005929 cilium 1
Pathway
R-HSA-1266738 Developmental Biology 3 R-HSA-162582 Signal Transduction 3 R-HSA-1640170 Cell Cycle 2 R-HSA-1852241 Organelle biogenesis and maintenance 2
Partners
ANK3CLIP1DRD2ITPR3MCAKPDE4DIPSIAH1STIM2

Evidence

Reading pass · 26 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 EB3 is a novel member of the EB1 family that associates with the cytoplasmic microtubule network and binds directly to APCL (APC2), a CNS-specific homologue of APC, as demonstrated by yeast two-hybrid screening and confocal microscopy showing co-localization in perinucleus and microtubule network. Yeast two-hybrid, confocal microscopy, sequence analysis Oncogene Medium 10644998
2003 EB3-GFP tracks exclusively to the growing plus-ends of microtubules in live neurons, marking microtubule growth events; microtubules grow slower in neurons than in glia/COS-1 cells, and ~65% of EB3-GFP movements in proximal dendrites are directed distally vs. ~35% toward the cell body, reflecting the mixed microtubule polarity of dendrites. Live-cell fluorescence microscopy with EB3-GFP in cultured neurons The Journal of neuroscience High 12684451
2005 EB1 and EB3 directly bind CLIP-170 and CLIP-115 through their C-terminal tyrosine residues, and control CLIP dissociation kinetics from microtubule plus-ends; RNAi depletion of EB1 and EB3 accelerates CLIP dissociation from tips, which is rescued by EB1 but not EB2 expression. RNA interference, direct binding assay, live-cell imaging of CLIP dynamics Molecular biology of the cell High 16148041
2007 EB3 is specifically upregulated upon myogenic differentiation; knockdown of EB3 (but not EB1) prevents myoblast elongation and fusion, impairs microtubule capture at the cell cortex, and disrupts microtubule dynamics; two specific amino acids in the calponin-like domain of EB3 are required for myoblast fusion. RNAi knockdown, live-cell imaging, EB1/EB3 chimera expression, C2C12 myoblast differentiation assay Current biology : CB High 17658256
2007 EB3 interacts with the p53-target DDA3 protein; the interaction requires intact microtubules, maps to aa 118-329 of DDA3 and both N- and C-termini of EB3; DDA3 and EB3 cooperate for microtubule binding in vitro; ectopic expression of DDA3 and EB3 enhances beta-catenin-dependent transactivation and cyclin D1 production. Yeast two-hybrid, GST pull-down, co-immunoprecipitation, in vitro microtubule-binding assay, immunofluorescence, reporter assay Oncogene Medium 17310996
2008 EB3 directly binds drebrin (an F-actin-associated protein); in growth cones this interaction occurs specifically when drebrin is on F-actin in the proximal region of filopodia and EB3 is at microtubule tips invading filopodia; disruption of this interaction impairs growth cone formation and neurite extension. Direct binding assay (in vitro), co-localization, dominant-negative disruption, neurite outgrowth assay Nature cell biology High 18806788
2009 EB3 stability is regulated during mitosis by Aurora-A and Aurora-B kinases that phosphorylate EB3 at Ser-176, disrupting the EB3-SIAH-1 complex; SIAH-1 ubiquitin ligase mediates EB3 polyubiquitination and proteasomal degradation during G1; phosphorylation-induced stabilization of EB3 during mitosis facilitates cell cycle progression at prometaphase. In vitro kinase assay, co-immunoprecipitation, RNAi knockdown, proteasome inhibition, cell cycle analysis The Journal of biological chemistry High 19696028
2010 In vitro reconstitution demonstrated that EB3 restores robust microtubule growth in the presence of MCAK (which alone blocks assembly) and targets MCAK to growing microtubule ends by increasing its association rate; the EB3-dependent targeting requires direct EB3-MCAK interaction and enhances MCAK's capacity to induce catastrophes without affecting growth/shortening velocities. In vitro reconstitution of microtubule dynamics, TIRF microscopy, quantitative analysis of dynamics Current biology : CB High 20850319
2011 EB3 (and EB1) directly interact with the AIS scaffold protein ankyrin G (ankG); EB3 is concentrated and stabilized at the axon initial segment (AIS) in mature neurons; knockdown of ankG leads to cell-wide upregulation of EB3 comets; EB3/EB1 participate in AIS maintenance. Direct interaction assay, live-cell imaging, immunofluorescence, ankG knockdown, fractionation/localization Proceedings of the National Academy of Sciences of the United States of America High 21551097
2011 EB3 (and EB1) are required for assembly of primary cilia; cells lacking EB1 or EB3 have defective MT minus-end anchoring at centrosome/basal body and short cilia stumps; GST pull-down and mass spectrometry showed EB1/EB3 interact with proteins implicated in MT minus-end anchoring and vesicular trafficking to cilia base; EB3 localizes to the tip of motile cilia and affects centriole-associated rootlet filament formation. Protein depletion (siRNA), dominant-negative expression, electron microscopy, GST pull-down, mass spectrometry, immunoprecipitation, live imaging Journal of cell science High 21768326
2011 The SH3 domain of PSD-95 interacts with a proline-rich region within EB3; overexpression of PSD-95 decreases the lifetime of EB3 comets in dendrites, leading to less organized microtubules at dendritic branch points and decreased dendritic branching. Co-immunoprecipitation, live-cell imaging (EB3 comet analysis), overexpression in neurons The Journal of neuroscience Medium 21248129
2012 VE-cadherin outside-in signaling activates Src and PLCγ2, causing Ca2+ release from ER stores, activating calcineurin (CaN); CaN downregulation leads to phosphorylation of EB3 at Ser-162, destabilizing the EB3 dimer, suppressing microtubule growth, and enabling adherens junction assembly; phospho-defective S162A EB3 mutant induces MT growth in confluent monolayers and disassembles AJs. Phospho-specific mutagenesis, calcium imaging, pharmacological inhibition, co-immunoprecipitation, endothelial permeability assay Molecular cell High 23159740
2012 Drebrin E and EB3 form a complex with myosin IIB and βII-spectrin at the apical domain of columnar epithelial cells; depletion of drebrin E disrupts apical accumulation of EB3 and impairs cell elongation; EB3 depletion produces a similar elongation defect; the complex connects F-actin and microtubule networks apically during epithelial morphogenesis. Co-immunoprecipitation, siRNA knockdown, immunofluorescence, morphometric analysis Journal of cell science Medium 22275434
2013 Aurora B phosphorylates EB3 at Ser-176 at the midbody to control cortical microtubule growth; EB3 stabilizes focal adhesions and coordinates daughter cell spreading during mitotic exit, promotes midbody microtubule stability, and is required for efficient cytokinesis; EB1 and EB3 play temporally distinct roles in cell division, with EB1 involved in spindle orientation before anaphase. Phospho-specific mutagenesis, live-cell imaging, RNAi knockdown, immunofluorescence The Journal of cell biology High 23712260
2014 BPAG1a/b C-terminal isoform-specific tails bind both EB1 and EB3 and are sufficient to bundle microtubules; knockdown of BPAG1a/b in C2.7 myoblasts impairs directness of cell migration and disrupts Golgi structure. GST pull-down, co-immunoprecipitation, microtubule bundling assay, siRNA knockdown, migration assay PloS one Medium 25244344
2015 EB3 binds to IP3 receptors (IP3R3) through an S/TxIP EB-binding motif; in endothelial cells, EB3 depletion or mutation of the TxIP motif of IP3R3 prevents α-thrombin-induced IP3R3 clustering, Ca2+ increase, myosin light chain phosphorylation, and vascular permeability increase; selective EB3 gene deletion in mouse endothelial cells abrogates α-thrombin-induced endothelial permeability. Co-immunoprecipitation, mutagenesis of binding motif, siRNA knockdown, conditional knockout mouse, Ca2+ imaging, permeability assay Cell reports High 26119739
2017 EB3 (and EB1) form a complex with myomegalin that acts as a membrane-microtubule tether at Golgi membranes; CRISPR/Cas9 knockout of EB2/EB3 and C-terminal half of EB1 reduces CAMSAP2-decorated microtubule minus end lengths, detaches microtubules from Golgi membranes, compacts the Golgi complex, and disrupts cell migration, polarity, and focal adhesion distribution. CRISPR/Cas9 knockout, co-immunoprecipitation, electron microscopy, live-cell imaging, immunofluorescence The Journal of cell biology High 28814570
2017 STIM2 forms a Ca2+-dependent complex with EB3 via a Ser-x-Ile-Pro (SxIP) amino acid motif; disruption of STIM2-EB3 interaction results in loss of mushroom dendritic spines; EB3 overexpression rescues mushroom spine loss in a PS1-M146V knock-in Alzheimer's disease model, while STIM2 overexpression fails to rescue spines after EB3 knockdown. Co-immunoprecipitation, mutagenesis of SxIP motif, siRNA knockdown, overexpression, spine morphometry in hippocampal neurons Scientific reports Medium 29247211
2018 IP3K-A (ITPKA) binds to EB3, and this interaction is regulated by PKA-dependent phosphorylation of IP3K-A at Ser119; the IP3K-A/EB3 complex dissociates and reassociates rapidly during chemically-induced LTP conditions. Co-immunoprecipitation, phospho-specific mutagenesis, chemical LTP induction Biochemical and biophysical research communications Low 30466786
2019 EB3 comet intensity profiles in mouse fibroblasts frequently exhibit secondary EB3-binding peaks (EB3-islands) on 56% of growing microtubule comets, which are stationary and originate from EB3 comets moving with growing MT tips; modeling suggests additional protein factors beyond GTP hydrolysis contribute to EB3 residence time on MTs. Live-cell fluorescence microscopy (EB3-RFP), quantitative image analysis, computational modeling Molecular biology of the cell Medium 31141458
2021 Loss of EB3 (but not EB1) inhibits neuritogenesis in embryonic cortical neurons; EB3 is more proximally located at dynamic microtubule plus-ends than EB1 in growth cone filopodia, enabling continuous microtubule elongation as the drebrin/EB3 pathway zippers microtubules to F-actin; pharmacological blockade of microtubule dynamics is associated with selective loss of EB3 (not EB1) from plus-ends. Gene editing (CRISPR), pharmacological inhibition, overexpression, live-cell imaging, morphometric analysis Journal of neurochemistry High 34478582
2023 Fidgetin (microtubule-severing enzyme) trims tyrosinated (labile) microtubules by interacting with EB3; fidgetin depletion increases EB3 at neurite ends, elongates the labile microtubule domain, and increases axon length and branching; EB3 knockdown does not change fidgetin expression but fidgetin deletion markedly increases EB3 levels. RNA interference, overexpression, rat SCI model, immunofluorescence, EB3 localization analysis Neural regeneration research Medium 37449637
2023 A 14-amino acid peptide (CIPRI) targeting the EB3-IP3R3 interface disrupts EB3-IP3R3 interaction in vitro and in mouse lungs; disruption mitigates Ca2+ release from ER stores, prevents VE-cadherin junction disassembly, and reduces inflammation-induced lung injury and microvascular leakage in mice. Peptide competition assay, co-immunoprecipitation, Ca2+ imaging, in vivo endotoxemia/sepsis mouse model, vascular permeability assay American journal of respiratory cell and molecular biology High 37290041
2024 EZH2-mediated H3K27 trimethylation epigenetically represses MAPRE3 (EB3) expression at its promoter in ovarian cancer; EZH2 knockdown decreases H3K27me3 enrichment at the MAPRE3 promoter and increases EB3 expression; MAPRE3 overexpression induces cell cycle arrest and apoptosis in ovarian cancer cells. ChIP assay (H3K27me3 at MAPRE3 promoter), EZH2 knockdown, overexpression, xenograft tumor model Experimental cell research Medium 38199479
2024 EB3 undergoes liquid-liquid phase separation (LLPS) with significantly higher propensity than EB1 despite 67% sequence identity; this difference involves contributions from multiple protein regions with histidine residues in the N-terminal domain playing a key role; EB3 condensates have lower dynamics (less fluid) than EB1 and higher capacity to recruit tubulin and nucleate polymerization; EB3/CLIP-170 droplets show higher tubulin polymerization capacity than EB1/CLIP-170. In vitro LLPS assay, fluorescence recovery after photobleaching (FRAP), mutagenesis, in vitro tubulin polymerization assay, cell-based condensate imaging The Journal of biological chemistry High 41135671
2024 In vitro reconstitution showed that EB3-dependent comet size at microtubule ends is independent of GTP-cap size; a phenomenological model based on noisy microtubule growth and a single EB3-dependent hydrolysis rate fully describes stalled and freely growing microtubule lifetime distributions; modulation of microtubule growth velocity in cells does not consistently alter EB3 comet length. In vitro reconstitution with microfabricated barriers, GFP-EB3 live imaging, mathematical modeling Biophysical journal Medium 39604262

Source papers

Stage 0 corpus · 38 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2003 Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein). The Journal of neuroscience : the official journal of the Society for Neuroscience 540 12684451
2008 Targeting of the F-actin-binding protein drebrin by the microtubule plus-tip protein EB3 is required for neuritogenesis. Nature cell biology 198 18806788
2005 EB1 and EB3 control CLIP dissociation from the ends of growing microtubules. Molecular biology of the cell 165 16148041
2011 End-binding proteins EB3 and EB1 link microtubules to ankyrin G in the axon initial segment. Proceedings of the National Academy of Sciences of the United States of America 131 21551097
2010 In vitro reconstitution of the functional interplay between MCAK and EB3 at microtubule plus ends. Current biology : CB 122 20850319
2000 EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue. Oncogene 106 10644998
2011 EB1 and EB3 promote cilia biogenesis by several centrosome-related mechanisms. Journal of cell science 94 21768326
2007 EB3 regulates microtubule dynamics at the cell cortex and is required for myoblast elongation and fusion. Current biology : CB 85 17658256
2017 EB1 and EB3 regulate microtubule minus end organization and Golgi morphology. The Journal of cell biology 64 28814570
1966 Partial purification and electron microscopy of virus in the EB-3 cell line derived from a Burkitt lymphoma. Science (New York, N.Y.) 57 5931456
2009 Mitotic regulation of the stability of microtubule plus-end tracking protein EB3 by ubiquitin ligase SIAH-1 and Aurora mitotic kinases. The Journal of biological chemistry 48 19696028
2017 Stim2-Eb3 Association and Morphology of Dendritic Spines in Hippocampal Neurons. Scientific reports 46 29247211
2011 PSD-95 alters microtubule dynamics via an association with EB3. The Journal of neuroscience : the official journal of the Society for Neuroscience 44 21248129
2007 p53 downstream target DDA3 is a novel microtubule-associated protein that interacts with end-binding protein EB3 and activates beta-catenin pathway. Oncogene 44 17310996
2012 VE-cadherin signaling induces EB3 phosphorylation to suppress microtubule growth and assemble adherens junctions. Molecular cell 43 23159740
2013 Aurora B spatially regulates EB3 phosphorylation to coordinate daughter cell adhesion with cytokinesis. The Journal of cell biology 41 23712260
2015 Microtubule-Associated Protein EB3 Regulates IP3 Receptor Clustering and Ca(2+) Signaling in Endothelial Cells. Cell reports 37 26119739
2012 Apico-basal elongation requires a drebrin-E-EB3 complex in columnar human epithelial cells. Journal of cell science 35 22275434
2008 Production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by bacillus pumilus EB3. Journal of bioscience and bioengineering 35 18929997
2016 The role of the drebrin/EB3/Cdk5 pathway in dendritic spine plasticity, implications for Alzheimer's disease. Brain research bulletin 29 27365229
2014 BPAG1a and b associate with EB1 and EB3 and modulate vesicular transport, Golgi apparatus structure, and cell migration in C2.7 myoblasts. PloS one 27 25244344
2019 Synaptic Microtubule-Associated Protein EB3 and SRC Phosphorylation Mediate Structural and Behavioral Adaptations During Withdrawal From Cocaine Self-Administration. The Journal of neuroscience : the official journal of the Society for Neuroscience 25 31092585
2014 Peptide aptamers define distinct EB1- and EB3-binding motifs and interfere with microtubule dynamics. Molecular biology of the cell 17 24478452
2017 The drebrin/EB3 pathway drives invasive activity in prostate cancer. Oncogene 16 28319065
2019 Fine structure and dynamics of EB3 binding zones on microtubules in fibroblast cells. Molecular biology of the cell 13 31141458
2021 The drebrin/EB3 pathway regulates cytoskeletal dynamics to drive neuritogenesis in embryonic cortical neurons. Journal of neurochemistry 12 34478582
2023 Fidgetin interacting with microtubule end binding protein EB3 affects axonal regrowth in spinal cord injury. Neural regeneration research 10 37449637
1976 Use of a transfection method to demonstrate a monolayer cell transforming agent from the EB3 line of Burkitt's lymphoma cells. The Journal of general virology 9 180248
2022 Cytoskeleton Protein EB3 Contributes to Dendritic Spines Enlargement and Enhances Their Resilience to Toxic Effects of Beta-Amyloid. International journal of molecular sciences 8 35216391
2023 Targeting EB3-IP3R3 Interface with Cognate Peptide Protects from Acute Respiratory Distress Syndrome. American journal of respiratory cell and molecular biology 7 37290041
2021 On the Relationship Between EB-3 Profiles and Microtubules Growth in Cultured Cells. Frontiers in molecular biosciences 5 34820422
2024 MAPRE3 as an epigenetic target of EZH2 restricts ovarian cancer proliferation in vitro and in vivo. Experimental cell research 3 38199479
2019 Enhancement of connexin30.3 expression in mouse embryonic stem cell line EB3 in response to cell-cell contacts. Human cell 2 30674001
2018 PKA-dependent phosphorylation of IP3K-A at Ser119 regulates a binding affinity with EB3. Biochemical and biophysical research communications 1 30466786
2026 Early Isoflurane Exposure Is Associated With Delayed Adolescent Neurobehavioral Deficits, Accompanied by Dysregulation of FOXG1 and EB3. Developmental neurobiology 0 42063249
2025 Distinct liquid-liquid phase separation properties of end-binding proteins EB1 and EB3. The Journal of biological chemistry 0 41135671
2024 Aspongopyrimidine A, a N-Peralkylated Histidine Zwitterion from Aspongopus chinensis against Alzheimer's Disease Targeting MAPRE3. Organic letters 0 38345436
2024 EB3-informed dynamics of the microtubule stabilizing cap during stalled growth. Biophysical journal 0 39604262

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