Affinage

LDHB

L-lactate dehydrogenase B chain · UniProt P07195

Length
334 aa
Mass
36.6 kDa
Annotated
2026-06-10
79 papers in source corpus 31 papers cited in narrative 31 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

LDHB is a lactate dehydrogenase subunit that interconverts lactate and pyruvate with coupled NAD+/NADH cycling, positioning it as a control point that routes carbon and redox equivalents into mitochondrial oxidative metabolism rather than fermentation (PMID:38607916, PMID:42217683). A conserved active-site Arg106 in a substrate-closing loop is required for catalysis, and the enzyme assembles into heterotetramers with LDHA subunits (PMID:8611651). Its directionality and activity are tuned by post-translational modifications: Aurora-A phosphorylation at S162 relieves pyruvate substrate inhibition to drive pyruvate-to-lactate flux and the Warburg effect (PMID:31804482), SIRT5-mediated deacetylation at K329 enhances activity and supports autophagy (PMID:30443978), EP300/HDAC2-regulated K156 lactylation amplifies NLRP3 inflammasome activation downstream of cGAS-STING signaling (PMID:41796892), and UCHL1-mediated deubiquitination stabilizes the protein (PMID:41998400). LDHB controls a lactate-to-pyruvate-to-acetyl-CoA route that fuels the TCA cycle and ATP production, sustains NAD+/NADH redox homeostasis through the malate-aspartate shuttle, and supports nucleotide biosynthesis and DNA-damage repair, such that its loss impairs metastatic capacity and sensitizes tumor cells to radiotherapy and cisplatin (PMID:40158058, PMID:41577157, PMID:42117986, PMID:40790017). By governing lactate levels, LDHB also feeds histone H3K18 lactylation at the PD-L1 promoter to promote immune evasion (PMID:39587817) and shapes cell-type-specific physiology including beta-cell insulin secretion (PMID:38607916), Schwann-cell-dependent motor function [PMID:bio_10.1101_2025.11.24.690227], and CD4 T-cell lactate tolerance (PMID:35682650). Its expression is set by transcriptional inputs (STAT3, YBX1, PGC-1alpha, and FGF-STAT1 signaling that suppresses LDHB) and post-transcriptional control via the FTO/m6A/YTHDF2 axis and promoter methylation (PMID:33434505, PMID:38381377, PMID:38810904, PMID:41577157, PMID:38764020, PMID:25807933). LDHB additionally serves as a host target hijacked by viral proteins from SARS-CoV-2, CSFV, and PDCoV (PMID:39147351, PMID:32924761, PMID:40231829).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1996 Medium

    Established the catalytic basis of LDHB activity by showing a single active-site residue is essential for substrate turnover.

    Evidence Sequencing of the natural LDHB GUA1 Arg106Trp variant with crystal-structure modeling and heterotetramer kinetics

    PMID:8611651

    Open questions at the time
    • No in vitro mutagenesis to isolate the residue's contribution
    • Did not address regulation of the enzyme in cells
  2. 2015 Medium

    Identified LDHB as a context-dependent suppressor of glycolysis whose silencing via promoter hypermethylation drives a glycolytic, pro-tumorigenic switch.

    Evidence Promoter methylation analysis with knockdown/overexpression and glycolysis assays in pancreatic cancer

    PMID:25807933

    Open questions at the time
    • Directionality of LDHB flux not directly measured
    • Mechanism linking methylation to phenotype indirect
  3. 2018 High

    Revealed that LDHB activity is acetylation-regulated, connecting a sirtuin to lactate metabolism and autophagy.

    Evidence MS partner identification, Co-IP, SIRT5 KO/inhibition and enzymatic assays in colorectal cancer

    PMID:30443978

    Open questions at the time
    • How K329 acetylation alters enzyme conformation unresolved
    • Link between activity and autophagy mechanistically incomplete
  4. 2019 High

    Showed LDHB directionality is switchable by phosphorylation, explaining how a 'lactate-to-pyruvate' enzyme can fuel the Warburg effect.

    Evidence Co-IP, in vitro Aurora-A kinase assay, S162A mutant, xenografts and flux measurements

    PMID:31804482

    Open questions at the time
    • Whether S162 phosphorylation operates outside tumor contexts unknown
    • Structural basis for relief of substrate inhibition not solved
  5. 2021 High

    Placed LDHB downstream of an RNA-modification axis, defining a post-transcriptional layer of glycolytic control.

    Evidence shRNA, overexpression rescue and m6A analysis in AML cells and in vivo

    PMID:33434505

    Open questions at the time
    • Direct YTHDF2-LDHB transcript engagement not fully mapped
  6. 2024 Medium

    Defined multiple transcriptional/post-transcriptional regulators (STAT3, PGC-1alpha, FGF-STAT1) and downstream effectors (MDH2, GSH catabolism), expanding LDHB's regulatory network and metabolic outputs.

    Evidence ChIP/luciferase, Co-IP, overexpression and metabolic-inhibitor rescue across endometrial, prostate, lung and liver models

    PMID:38381377 PMID:38764020 PMID:38810904 PMID:39615645

    Open questions at the time
    • Each regulator shown in a single context
    • Whether these inputs converge in normal tissue unclear
  7. 2024 Medium

    Identified non-catalytic protein-interaction roles of LDHB in lysosomal acidification and mitochondrial TFAM degradation, broadening its function beyond enzymatic activity.

    Evidence Co-IP with interaction-residue mutagenesis (ATP6V1A L57/LDHB S269), LONP1 inhibition and in vivo vascular/atherosclerosis models

    PMID:39647238 PMID:39731912

    Open questions at the time
    • Whether these scaffolding roles are independent of catalysis untested
    • Single-lab findings without reciprocal validation
  8. 2024 Medium

    Established LDHB as a determinant of cell-type-specific physiology and immune behavior through control of lactate levels.

    Evidence 13C tracing and inhibition in human/mouse islets, ChIP-qPCR of H3K18 lactylation at PD-L1, T cell co-culture and Mendelian randomization

    PMID:35682650 PMID:38607916 PMID:39587817

    Open questions at the time
    • Causal direction of lactylation epigenetics needs further mutant validation
    • Islet effect mechanism partly genetic-correlative
  9. 2024 Medium

    Defined LDHB as host machinery exploited or inhibited by viral proteins linking it to antiviral metabolism and autophagy.

    Evidence AP-MS/Y2H, Co-IP, mitophagy/autophagy reporters and viral titer assays for SARS-CoV-2 S1, CSFV NS3 and PDCoV N

    PMID:32924761 PMID:39147351 PMID:40231829

    Open questions at the time
    • Physiological relevance of metabolic switch during infection unclear
    • Interaction interfaces only partly mapped
  10. 2025 Medium

    Linked LDHB-dependent nucleotide synthesis to DNA-damage repair and therapeutic sensitivity, providing a basis for combining LDHB inhibition with radio/chemotherapy.

    Evidence siRNA/shRNA silencing, gammaH2AX, metabolomics and nucleotide-supplementation rescue in lung and mesothelioma xenografts

    PMID:40158058 PMID:40790017

    Open questions at the time
    • Step connecting LDHB metabolism to nucleotide pools indirect
    • Whether effect generalizes beyond these tumor types unknown
  11. 2025 Medium

    Demonstrated tissue-specific in vivo requirements for LDHB, including Schwann-cell-dependent motor function and immune lactate sensing.

    Evidence Cell-type-specific conditional knockouts, NMJ histology and ALS epistasis (preprint); TLR7/9-driven LDHB repression in SLE neutrophils (preprint)

    PMID:41279671 PMID:bio_10.1101_2025.11.24.690227

    Open questions at the time
    • Preprints awaiting peer review
    • Molecular mechanism of LDHB in Schwann-cell support undefined
  12. 2026 Medium

    Resolved how LDHB protein stability and lactylation feed into inflammasome activation, redox-metabolic axes, and stromal reprogramming.

    Evidence UCHL1 deubiquitination assays, K156R lactylation-deficient mutant rescue in sepsis, YBX1 feed-forward loop, DUSP16-p38 Co-IP and LDHB-MPC-NAD+ axis in BBB endothelium

    PMID:41577157 PMID:41686427 PMID:41796892 PMID:41998400 PMID:42217683

    Open questions at the time
    • Each mechanism shown in one disease context
    • Crosstalk among PTMs not integrated
  13. 2026 Medium

    Showed maternal LDHB is required for redox homeostasis driving early embryonic development.

    Evidence Pharmacological inhibition in mouse embryos with NAD+/NADH and ATP readouts and aspartate (malate-aspartate shuttle) rescue

    PMID:42117986

    Open questions at the time
    • Genetic loss-of-function not performed
    • Specific developmental targets of redox imbalance undefined

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the diverse post-translational modifications (phosphorylation, acetylation, lactylation, ubiquitination) are integrated to set LDHB flux directionality in any single physiological tissue remains unresolved.
  • No structural model of the modified enzyme states
  • No study reconciles competing context-dependent roles as glycolytic promoter vs suppressor

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016491 oxidoreductase activity 4 GO:0140098 catalytic activity, acting on RNA 4
Localization
GO:0005739 mitochondrion 3 GO:0005829 cytosol 2
Pathway
R-HSA-1430728 Metabolism 5 R-HSA-8953854 Metabolism of RNA 3 R-HSA-9612973 Autophagy 3
Partners
ATP6V1AAURKAHMOX1LDHAMDH2SIRT5UCHL1YBX1

Evidence

Reading pass · 31 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2019 Aurora-A kinase directly interacts with and phosphorylates LDHB at serine 162, which increases LDHB activity in reducing pyruvate to lactate. Mechanistically, S162 phosphorylation relieves substrate inhibition by pyruvate, resulting in elevated conversion of pyruvate and NADH to lactate and NAD+, thereby promoting glycolytic flux and the Warburg effect. Expression of LDHB-S162A mutant blocked glycolysis and tumor growth. Co-IP, in vitro kinase assay, site-directed mutagenesis (S162A), xenograft tumor models, metabolic flux measurements Nature communications High 31804482
2018 SIRT5 binds LDHB and deacetylates it at lysine-329, thereby promoting LDHB enzymatic activity. Deacetylated LDHB increases autophagy and colorectal cancer cell growth. SIRT5 knockout or inhibition increased LDHB acetylation at K329 and inhibited LDHB activity, downregulating autophagy and cancer cell growth. Mass spectrometry identification of SIRT5 as binding partner, Co-IP, SIRT5 knockout/inhibition, enzymatic activity assays, in vitro and in vivo tumor growth assays Molecular oncology High 30443978
2021 R-2-hydroxyglutarate (R-2HG) suppresses aerobic glycolysis in leukemia by abrogating FTO/m6A/YTHDF2-mediated post-transcriptional upregulation of LDHB expression. Knockdown of LDHB recapitulates R-2HG-induced glycolytic inhibition, and LDHB overexpression reverses the R-2HG effect, placing LDHB downstream of the FTO/m6A axis in glycolytic regulation. shRNA knockdown, overexpression rescue experiments, m6A modification analysis, in vivo leukemogenesis model Molecular cell High 33434505
2024 HMOX1 interacts with LDHB in foamy macrophages during advanced atherosclerosis. This HMOX1-LDHB interaction enables LONP1 to degrade mitochondrial transcription factor TFAM, leading to mitochondrial dysfunction and ferroptosis. Bulk and single-cell RNA sequencing, protein interaction studies, LONP1 inhibitor experiments, MitoTEMPO treatment in vivo Developmental cell Medium 39731912
2024 LDHB inhibition in lung cancer cells decreases total intracellular glutathione (GSH) levels, specifically reducing mitochondrial GSH catabolism by gamma-L-Glutamyl transpeptidase (GGT). GSH-monoethyl ester supplementation partially rescued the reduced invasion, migration, and colony formation caused by LDHB silencing, establishing that LDHB supports metastatic potential through mitochondrial GSH catabolism. siRNA silencing, metabolic inhibitor experiments, GSH supplementation rescue, in vivo metastasis models in immunodeficient and immunocompetent mice Cancer letters Medium 39615645
2024 LDHB interacts with vacuolar-type proton ATPase catalytic subunit A (ATP6V1A), promoting lysosomal acidification and autophagic flux. The residues leucine 57 in ATP6V1A and serine 269 in LDHB are critical for their interaction. Hydrogen sulfide (H2S) donor NaHS attenuates disturbed flow-induced vascular remodeling by inhibiting LDHB and disrupting the LDHB-ATP6V1A interaction. RNA-Seq, Co-IP, mutagenesis of interaction residues (Leu57, Ser269), LDHB overexpression rescue, in vivo vascular remodeling model Redox biology Medium 39647238
2024 LDHB has β cell-specific expression in human islets and limits lactate generation. LDHB inhibition amplifies LDHA-dependent lactate generation and increases basal insulin release in both mouse and human β cells. Mendelian randomization shows that low LDHB expression correlates with elevated fasting insulin. 13C6 glucose labeling with GC-MS and 2D NMR metabolic mapping, LDHB inhibition in islets, Mendelian randomization Cell reports High 38607916
2024 STAT3 transcription factor binds the promoter region of LDHB and activates its transcription in endometrial cancer cells. LDHB in turn interacts with and upregulates MDH2 expression, promoting cancer cell malignancy. ChIP assay, dual-luciferase reporter assay, Co-IP, LDHB and STAT3 knockdown with phenotypic rescue by MDH2 overexpression Molecular biotechnology Medium 38381377
2024 LDHB modulates lactate production and histone H3K18 lactylation at the PD-L1 promoter to promote PD-L1 expression and immune evasion in ovarian cancer. LDHB knockdown reduced H3K18 lactylation at the PD-L1 promoter and decreased PD-L1 expression, enhancing T cell killing. ChIP-qPCR, luciferase reporter assay, LDHB siRNA knockdown, T cell co-culture cytotoxicity assay, ELISA for immune factors Cancer investigation Medium 39587817
2024 SARS-CoV-2 Spike S1 domain interacts with LDHB and inhibits its catalytic activity, leading to increased lactate levels and a metabolic switch from aerobic to anaerobic metabolism. The Spike-NAD+ interacting region mainly involves W436 within the RBD domain, suggesting Spike deprives LDHB of NAD+. AP-MS interactome, Co-IP, immunofluorescence colocalization, enzymatic activity assay in HEK-293T cells overexpressing S1 International journal of biological macromolecules Medium 39147351
2024 PGC-1α transcriptionally upregulates LDHB synthesis; PGC-1α overexpression increases LDHB expression, reduces protein lactylation, and induces a switch from lactate to pyruvate production in APAP-induced liver injury model. Lentiviral overexpression of SIRT1 and PGC-1α, Western blot, measurement of lactylation and mitochondrial damage markers in AML12 cells and C57/BL6 mice Pharmacological research Medium 38810904
2024 LDHB silencing in lung cancer cells decreases nucleotide metabolism (purine and pyrimidine biosynthesis) as shown by metabolomics of tumor xenografts, impairs DNA damage repair, and sensitizes cells to radiotherapy. Nucleotide supplementation partially rescued DNA damage caused by combined LDHB silencing and radiotherapy. Transcriptomic re-analysis, γH2AX immunofluorescence, cell cycle analysis, metabolomics of xenografts, nucleotide supplementation rescue Scientific reports Medium 40158058
2025 In SLE neutrophils, chronic TLR7/9 signaling represses mitochondrial LDHB expression, impairing lactate sensing and suicidal NETosis; neutrophils instead default to vital NET release. Restoring LDHB expression (via HCQ + IFNAR blockade) re-establishes suicidal NETosis and bacterial clearance. Lupus-prone mouse model, LDHB expression analysis, HCQ and anifrolumab treatment in SLE patient neutrophils, NETosis assays bioRxivpreprint Medium 41279671
2026 LDHB deficiency in cancer-associated fibroblasts (CAFs) leads to lactate accumulation, which disrupts DUSP16-p38 interaction, causing sustained p38 activation. This reprograms CAFs into an inflammatory phenotype with CXCL8 secretion that enhances breast cancer metastasis. LDHB knockout in CAFs, Co-IP for DUSP16-p38 interaction, CXCL8 secretion assay, in vivo metastasis model Cancer research Medium 41686427
2026 UCHL1 deubiquitinase binds LDHB and deubiquitinates it, thereby stabilizing LDHB protein and promoting osteosarcoma progression. LDHB knockdown reversed UCHL1-driven oncogenesis. Co-immunoprecipitation, ubiquitination assay, shRNA knockdown of UCHL1 and LDHB, xenograft model Discover oncology Medium 41998400
2026 LDHB K156 lactylation, regulated by the EP300/HDAC2 axis, is induced downstream of cGAS-STING-mediated glycolytic reprogramming in sepsis, amplifying NLRP3 inflammasome activation and renal injury. AAV-mediated expression of K156R lactylation-deficient LDHB reduced renal dysfunction compared to wild-type LDHB. Lactyl-proteomic screening, CLP mouse model, AAV-mediated tubule-specific expression of WT vs K156R LDHB, biochemical assays for EP300/HDAC2 regulation Life sciences Medium 41796892
2026 In blood-brain barrier endothelial cells, extracellular lactate upregulates LDHB, MPC1, MPC2, and PDH, driving lactate-derived pyruvate into mitochondria and fueling TCA cycle and oxidative phosphorylation. Genetic silencing of LDHB abolishes lactate-driven BEC proliferation, establishing LDHB as the entry point of an LDHB-MPC-NAD+ redox-metabolic axis. LDHB siRNA silencing, high-resolution respirometry, MPC1 inhibition, NAMPT inhibition, glucose uptake and GLUT1 measurements Free radical biology & medicine Medium 42217683
2025 In Schwann cells (peripheral nerve glia), LDHB is required for motor function: SC-specific LDHB deletion caused robust motor defects, whereas motor neuron-specific deletion had little effect. In addition, Ldhb knockout mice develop progressive neuromuscular junction atrophy. Motor-neuron LDHB deficiency synergizes with ALS risk variants (TDP43-Q331K, Sod1-D83G) to produce early motor neuropathy. Cell-type-specific conditional LDHB knockout (Schwann cell vs motor neuron), neuromuscular junction histology, motor behavior assays, ALS knock-in allele genetic epistasis bioRxivpreprint Medium bio_10.1101_2025.11.24.690227
2026 YBX1 promotes LDHB expression by increasing LDHB transcriptional activity and stabilizing LDHB mRNA. Elevated LDHB drives conversion of lactate to pyruvate and then acetyl-CoA, enhancing TCA cycle activity and ATP production in intrahepatic cholangiocarcinoma cells. Lactate induces YBX1 nuclear translocation, which activates LDHB transcription in a feed-forward loop. YBX1 overexpression/knockout (CRISPR-Cas9), LDHB knockout, mRNA stability assays, metabolic flux measurements (TCA cycle activity, ATP), in vitro and in vivo tumor growth Pharmacological research Medium 41577157
1996 A naturally occurring LDHB variant (LDHB GUA1) carries an Arg-to-Trp substitution at residue 106 in the active site loop. This Arg106 residue is conserved across evolution and resides in the hinge of a loop that closes over the active site upon substrate binding; the substitution abolishes catalytic activity by preventing polarization of the substrate carbonyl bond, while maintaining normal kinetic properties in heterotetramers with active LDHA subunits. DNA sequencing identifying C→T transition (Arg106Trp), computer modeling using published crystal structures, kinetic analysis of heterotetramers Biochimica et biophysica acta Medium 8611651
2020 LDHB interacts with CSFV non-structural protein NS3. LDHB knockdown induces mitochondrial fission and mitophagy (evidenced by decreased TOMM20 and VDAC1, and promoted ubiquitination of MFN2) and promotes NFKB signaling, creating conditions conducive to viral persistence. LDHB overexpression decreased CSFV replication. Yeast two-hybrid screening, Co-IP, GST pulldown, confocal colocalization, siRNA knockdown, dual fluorescence mitophagy reporter (mito-mRFP-EGFP), viral titer assays Autophagy Medium 32924761
2024 FGF1/2 signaling positively regulates STAT1, which transcriptionally activates LDHA expression while suppressing LDHB expression in prostate cancer cells, thereby promoting glycolysis. RT-qPCR, Western blot, ECAR glycolysis measurements, FGF pathway inhibitor in xenograft model, STAT1 knockdown/overexpression Journal of translational medicine Medium 38764020
2026 Maternal LDHB is required for NAD+/NADH redox homeostasis during the 4- to 8-cell transition in preimplantation embryo development. Inhibition of LDHB caused developmental arrest, reduced ATP, impaired mitochondrial function, and decreased NAD+/NADH ratio. Aspartate supplementation rescued developmental progression via the malate-aspartate shuttle. Transient pharmacological inhibition of LDHB in early mouse embryos, metabolic readouts (ATP, NAD+/NADH), aspartate supplementation rescue, mitochondrial function assays FASEB journal Medium 42117986
2025 LDHB silencing in pleural mesothelioma cells increases nuclear DNA damage (elevated γH2AX) by impairing nucleotide synthesis, which is reversed by nucleotide supplementation. LDHB inhibition reduced tumor growth in vivo and enhanced cisplatin efficacy. siRNA and inducible shRNA silencing, γH2AX measurement, nucleotide supplementation rescue, in vivo xenograft with cisplatin combination Oncogenesis Medium 40790017
2015 LDHB promoter hypermethylation suppresses LDHB expression in pancreatic cancer, leading to glycolytic transition. Decreased LDHB expression promotes proliferation, invasion, and migration in hypoxia, establishing LDHB as a suppressor of glycolysis in this context. Promoter methylation analysis, LDHB knockdown/overexpression, functional assays (proliferation, invasion, migration), glycolysis measurements Medical oncology Medium 25807933
2022 LDHB overexpression in CD4 T cells increases cell respiration and mitigates lactic acid-induced inhibition of intracellular cytokine production. LDHB-overexpressing T cells preferentially migrated into HCT116 tumor spheroids and displayed higher expression of cytotoxic effector molecules. LDHB overexpression in human CD4 T cells, Seahorse metabolic assay, tumor spheroid migration assay, flow cytometry for cytokine and effector molecule expression International journal of molecular sciences Medium 35682650
2025 Mechanical stress activates IER3, which upregulates LDHB (identified as downstream target by mass spectrometry). LDHB promotes lactate production and lactylation in BMSCs, which drives osteogenic differentiation. IER3 inhibition blocked mechanical stress-induced increases in lactate and lactylation. Mass spectrometry identification of LDHB as IER3 target, uniaxial cell stretching system, IER3 knockdown with lactylation and differentiation assays FASEB journal Low 40244862
2025 Visomitin directly targets STAT3, inhibiting its transcriptional activity and thereby modulating LDHB expression levels in osteoclasts, triggering metabolic reprogramming and reducing osteoclastogenesis. STAT3 targeting assay, LDHB expression analysis, osteoclastogenesis assays, in vivo bone loss model Research (Washington, D.C.) Low 40698330
2025 Luteolin and quercetin exhibit uncompetitive inhibition of LDHB by binding at an allosteric site at the dimer interface, distinct from the active site where they competitively inhibit LDHA. This was supported by enzyme kinetic assays and molecular docking. In vitro enzyme kinetic assays (Ki determination, inhibition mode), molecular docking, virtual screening of 115 compounds Molecules (Basel, Switzerland) Low 40733189
2021 HYOU1 promotes LDHB expression at the post-transcriptional level by stabilizing LDHB mRNA through suppression of miR-375-3p levels. LDHB overexpression rescued the inhibitory effects of HYOU1 silencing on glycolysis, proliferation, and invasion in papillary thyroid cancer cells. HYOU1 siRNA silencing, miR-375-3p measurement, 3'UTR targeting analysis, LDHB overexpression rescue of glycolysis and proliferation Journal of cellular and molecular medicine Low 33792181
2025 LDHB directly interacts with PDCoV nucleocapsid (N) protein in the cytoplasm and mediates autophagic degradation of the N protein, thereby suppressing viral replication. PDCoV N protein, via its LIR motif, binds LC3 and facilitates LDHB degradation as a viral immune evasion strategy. Co-IP, yeast two-hybrid, confocal colocalization, siRNA knockdown, LDHB overexpression, viral titer assays, autophagy flux assays Microbiology spectrum Medium 40231829

Source papers

Stage 0 corpus · 79 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2021 R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis. Molecular cell 256 33434505
2019 Unappreciated Role of LDHA and LDHB to Control Apoptosis and Autophagy in Tumor Cells. International journal of molecular sciences 232 31035592
2012 An integrated genomic screen identifies LDHB as an essential gene for triple-negative breast cancer. Cancer research 165 23139210
2018 SIRT5-mediated deacetylation of LDHB promotes autophagy and tumorigenesis in colorectal cancer. Molecular oncology 112 30443978
2019 Aurora-A mediated phosphorylation of LDHB promotes glycolysis and tumor progression by relieving the substrate-inhibition effect. Nature communications 107 31804482
1978 Biochemical genetics of Fundulus heterolitus (L.). I. Temporal and spatial variation in gene frequencies of Ldh-B, Mdh-A, Gpi-B, and Pgm-A. Biochemical genetics 76 736886
2018 HMGB2 is associated with malignancy and regulates Warburg effect by targeting LDHB and FBP1 in breast cancer. Cell communication and signaling : CCS 75 29463261
2020 LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection. Autophagy 70 32924761
2015 Suppressed expression of LDHB promotes pancreatic cancer progression via inducing glycolytic phenotype. Medical oncology (Northwood, London, England) 58 25807933
2003 IS981-mediated adaptive evolution recovers lactate production by ldhB transcription activation in a lactate dehydrogenase-deficient strain of Lactococcus lactis. Journal of bacteriology 57 12867459
1982 Role of the Ek molecule in the generation of suppressor T cells in response to LDHB. Scandinavian journal of immunology 57 6214845
1997 Structural and functional differences in the promoter and 5' flanking region of Ldh-B within and between populations of the teleost Fundulus heteroclitus. Genetics 54 9055085
1987 Tetrasomy for the short arm of chromosome 12 with accessory isochromosome (+i(12p)) and a marked LDH-B gene dosage effect. Clinical genetics 48 3476222
2015 Exercise-induced changes in tumour LDH-B and MCT1 expression are modulated by oestrogen-related receptor alpha in breast cancer-bearing BALB/c mice. The Journal of physiology 45 25907793
2008 Molecular risk stratification of medulloblastoma patients based on immunohistochemical analysis of MYC, LDHB, and CCNB1 expression. Clinical cancer research : an official journal of the American Association for Cancer Research 45 18593994
2024 HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis. Developmental cell 42 39731912
2007 The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties - comparative modeling to probe the molecular basis. The FEBS journal 41 17944947
2024 PGC-1α loss promotes mitochondrial protein lactylation in acetaminophen-induced liver injury via the LDHB-lactate axis. Pharmacological research 40 38810904
2019 MiR-335-5p Inhibits Cell Proliferation, Migration and Invasion in Colorectal Cancer through Downregulating LDHB. Journal of B.U.ON. : official journal of the Balkan Union of Oncology 39 31424671
2019 The KLF14 Transcription Factor Regulates Glycolysis by Downregulating LDHB in Colorectal Cancer. International journal of biological sciences 38 30745849
1979 Regional assignment of human genes TPI1, GAPDH, LDHB, SHMT, and PEPB on chromosome 12. Cytogenetics and cell genetics 38 477403
1978 Genetic bases for protein polymorphism in Fundulus heteroclitus (L.).I. Lactate dehydrogenase (Ldh-B), malate dehydrogenase (Mdh-A), glucosephosphate isomerase (Gpi-B), and phosphoglucomutase (Pgm-A). Biochemical genetics 37 736885
2022 LDHB Deficiency Promotes Mitochondrial Dysfunction Mediated Oxidative Stress and Neurodegeneration in Adult Mouse Brain. Antioxidants (Basel, Switzerland) 35 35204143
2018 MiR-375 Regulation of LDHB Plays Distinct Roles in Polyomavirus-Positive and -Negative Merkel Cell Carcinoma. Cancers 31 30441870
2021 HYOU1 facilitates proliferation, invasion and glycolysis of papillary thyroid cancer via stabilizing LDHB mRNA. Journal of cellular and molecular medicine 30 33792181
1983 The use of enzyme kinetics to predict differences in cellular metabolism, developmental rate, and swimming performance between LDH-B genotypes of the fish, fundulus heteroclitus. Isozymes 30 6354981
1986 Further data on chromosomal assignments of pig enzyme loci LDHA, LDHB, MPI, PEPB and PGM1, using somatic cell hybrids. Animal genetics 29 2950811
1981 Resolution of breakpoints in a complex rearrangement by use of multiple staining techniques: confirmation of suspected 12p12.3 intraband by deletion dosage effect of LDHB. American journal of medical genetics 29 6167167
2020 Suppressed expression of LDHB promotes age-related hearing loss via aerobic glycolysis. Cell death & disease 28 32415082
2015 Low Expression of LDHB Correlates With Unfavorable Survival in Hepatocellular Carcinoma: Strobe-Compliant Article. Medicine 25 26426634
2024 LDHB Mediates Histone Lactylation to Activate PD-L1 and Promote Ovarian Cancer Immune Escape. Cancer investigation 24 39587817
1983 The nature of the interaction between suppressor and helper T cells in the response to LDHB. Journal of immunology (Baltimore, Md. : 1950) 24 6190936
2022 LDHB Overexpression Can Partially Overcome T Cell Inhibition by Lactic Acid. International journal of molecular sciences 23 35682650
1996 Descriptive and functional characterization of variation in the Fundulus heteroclitus Ldh-B proximal promoter. The Journal of experimental zoology 23 8691188
1989 Human testicular lactate dehydrogenase-C gene is interrupted by six introns at positions homologous to those of LDH-A (muscle) and LDH-B (heart) genes. Biochemical and biophysical research communications 20 2930531
2024 LDHB contributes to the regulation of lactate levels and basal insulin secretion in human pancreatic β cells. Cell reports 18 38607916
2024 Fibroblast growth factor pathway promotes glycolysis by activating LDHA and suppressing LDHB in a STAT1-dependent manner in prostate cancer. Journal of translational medicine 18 38764020
2020 ATPR triggers acute myeloid leukaemia cells differentiation and cycle arrest via the RARα/LDHB/ERK-glycolysis signalling axis. Journal of cellular and molecular medicine 15 32391634
2024 Epigenetic silencing of LDHB promotes hepatocellular carcinoma by remodeling the tumor microenvironment. Cancer immunology, immunotherapy : CII 14 38739169
2024 Decreased LDHB expression in breast tumor cells causes NK cell activation and promotes tumor progression. Cancer biology & medicine 13 38525901
2020 MiR-141-3p overexpression suppresses the malignancy of osteosarcoma by targeting FUS to degrade LDHB. Bioscience reports 11 32484203
2023 LDHA and LDHB overexpression promoted the Warburg effect in malignantly transformed GES-1 cells induced by N-nitroso compounds. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 10 37648104
2022 Slc2a6 regulates myoblast differentiation by targeting LDHB. Cell communication and signaling : CCS 9 35850889
1998 A monophyletic origin of heart-predominant lactate dehydrogenase (LDH) isozymes of gnathostome vertebrates: evidence from the cDNA sequence of the spiny dogfish (Squalus acanthias) LDH-B. Molecular marine biology and biotechnology 9 9628010
2016 Antibodies against Clonorchis sinensis LDH could cross-react with LDHB localizing on the plasma membrane of human hepatocarcinoma cell SMMC-7721 and induce apoptosis. Parasitology research 8 26769711
1987 [Creation of a clone panel of fox x Chinese hamster somatic cell hybrids and chromosome mapping of genes for LDHA, LDHB, GPI, ESD, G6PD, HPRT, alpha-GALA in the silver fox]. Genetika 8 3040528
2023 A study of the interaction space of two lactate dehydrogenase isoforms (LDHA and LDHB) and some of their inhibitors using proteochemometrics modeling. BMC chemistry 7 37415191
2021 Testicular germ cell tumors type 2 have high RNA expression of LDHB, the gene for lactate dehydrogenase subunit B. Asian journal of andrology 7 33565425
1983 Separation of the immune response genes for LDH-B and MOPC-173. I. Description of an immune response defect in B10.BASR1. Journal of immunology (Baltimore, Md. : 1950) 7 6417235
1972 Centric fusion and trisomy for the LDH-B locus in brook trout, Salvelinus fontinalis. Science (New York, N.Y.) 7 5084668
2024 Transcription Factor STAT3-Activated LDHB Promotes Tumor Properties of Endometrial Cancer Cells by Inducing MDH2 Expression. Molecular biotechnology 6 38381377
2024 Inhibition of LDHB suppresses the metastatic potential of lung cancer by reducing mitochondrial GSH catabolism. Cancer letters 6 39615645
2024 Hydrogen sulfide attenuates disturbed flow-induced vascular remodeling by inhibiting LDHB-mediated autophagic flux. Redox biology 6 39647238
2009 Comparative characterization of a temperature responsive gene (lactate dehydrogenase-B, ldh-b) in two congeneric tropical fish, Lates calcarifer and Lates niloticus. International journal of biological sciences 6 19787021
2025 LDHB silencing enhances the effects of radiotherapy by impairing nucleotide metabolism and promoting persistent DNA damage. Scientific reports 5 40158058
2025 Exercise-Mediated Mechanical Stress Promotes Osteogenic Differentiation of BMSCs Through Upregulation of Lactylation via the IER3/LDHB Axis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 5 40244862
2024 SARS-CoV-2 uses Spike glycoprotein to control the host's anaerobic metabolism by inhibiting LDHB. International journal of biological macromolecules 5 39147351
1996 Arginine to tryptophan substitution in the active site of a human lactate dehydrogenase variant--LDHB GUA1: postulated effects on subunit structure and catalysis. Biochimica et biophysica acta 5 8611651
2025 Flipping the Target: Evaluating Natural LDHA Inhibitors for Selective LDHB Modulation. Molecules (Basel, Switzerland) 4 40733189
2024 LDHB-deficient brain exhibits resistance to ischemic neuronal cell death due to increased vasodilation. Biochemical and biophysical research communications 4 39368368
2000 Differential performance among LDH-B genotypes in Rana lessonae tadpoles. Evolution; international journal of organic evolution 4 11108601
2025 Visomitin Attenuates Pathological Bone Loss by Reprogramming Osteoclast Metabolism via the STAT3/LDHB Axis. Research (Washington, D.C.) 3 40698330
2021 High expression of FBP1 and LDHB in fibroadenomas and invasive breast cancers. Breast disease 3 34092577
1992 Sheep gene mapping: synteny between COL2A1 and the LDHB-PEPB-TPI-GAPD-LALBA-IGF1 group. Animal genetics 3 1492704
1994 [Genetic defects in inheritance and the variability of locus Ldh-B in hybrid populations of Rana esculenta complex (Amphibia, Ranidae)]. Izvestiia Akademii nauk. Seriia biologicheskaia 2 7804099
1984 Separation of the immune response genes for LDH-B and MOPC-173. II. Description of an immune response defect in B10.ASR7. Journal of immunology (Baltimore, Md. : 1950) 2 6199419
2026 YBX1-LDHB axis orchestrates pyruvate production from lactate to promote ICC initiation and development. Pharmacological research 1 41577157
2026 LDHB K156 lactylation links cGAS-STING-mediated metabolic reprogramming to NLRP3 inflammasome activation in sepsis-associated acute kidney injury. Life sciences 1 41796892
2025 Fluorescence lifetime imaging microscopy for metabolic analysis of LDHB inhibition in triple negative breast cancer. bioRxiv : the preprint server for biology 1 39868164
2025 Inhibition of LDHB triggers DNA damage and increases cisplatin sensitivity in pleural mesothelioma. Oncogenesis 1 40790017
2026 LDHB Deficiency in Fibroblasts Induces Lactate-Mediated Inflammatory Reprogramming That Promotes Breast Cancer Metastasis. Cancer research 0 41686427
2026 UCHL1 promotes osteosarcoma progression via deubiquitination and stabilization of LDHB. Discover oncology 0 41998400
2026 Regulation of Histone Emulsification by HPDL via LDHA/LDHB Promotes EC Cell Proliferation. Oncology research 0 42065070
2026 Maternal LDHB Safeguards Redox Balance and Developmental Competence During Preimplantation Embryo Cleavage. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 0 42117986
2026 Extracellular lactate reprograms mitochondrial metabolism and NAD+/NADH redox balance in blood-brain barrier endothelial cells via an LDHB-MPC-NAD+ axis. Free radical biology & medicine 0 42217683
2025 LDHB suppresses the PDCoV proliferation by targeting viral nucleocapsid protein for autophagic degradation. Microbiology spectrum 0 40231829
2025 A TLR7/9-IFNα-LDHB axis drives vital NET release and compromises antibacterial defense in lupus. bioRxiv : the preprint server for biology 0 41279671
2024 Identification of Genetic Associations of IDH2, LDHA, and LDHB Genes with Milk Yield and Compositions in Dairy Cows. Life (Basel, Switzerland) 0 39459528
1985 Separation of the immune response genes for LDH-B and MOPC-173. III. Evidence that the failure of B10.BASR1 to respond to LDH-B is due to an antigen-presenting cell defect. Transplantation 0 2932822

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