Affinage

LCP1

Plastin-2 · UniProt P13796

Length
627 aa
Mass
70.3 kDa
Annotated
2026-06-10
100 papers in source corpus 47 papers cited in narrative 47 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

L-plastin (LCP1) is a leukocyte-enriched, calcium-regulated actin-bundling protein that cross-links F-actin into the higher-order structures driving immune cell migration, adhesion, and invasion (PMID:1491005, PMID:8461306). It binds laterally along the filament through two actin-binding domains of differing affinity, contacts beta-actin selectively, and bundles only at low free calcium (optimal below ~0.15 µM, progressively inhibited above), reflecting a calcium-dependent conformational switch in its EF-hand headpiece (PMID:1491005, PMID:8461306, PMID:14992580); an NMR structure localized this switch to a helix that binds the EF-hand motifs in the presence of calcium and governs bundling activity (PMID:28145401). The dominant activating modification is phosphorylation of Ser5 in the N-terminal headpiece, which shifts the protein to a high-affinity state—increasing the F-actin association rate roughly two-fold and lowering actin dissociation—and is sufficient to drive integrin inside-out activation and adhesion in neutrophils and monocytes (PMID:9689080, PMID:20169155, PMID:16636079). Ser5 is targeted by multiple kinases converging from distinct receptor inputs: PKA downstream of FcγR/PI3K/cAMP signaling (PMID:10446213), PKCβII in GM-CSF-primed eosinophils (PMID:21525390), RSK1/2 downstream of ERK/MAPK and SGK3 downstream of PI3K in cancer cells (PMID:26631483, PMID:33618712), and Mst1 on Thr89 (PMID:27465533), while dexamethasone suppresses Ser5 phosphorylation to blunt T cell synapse maturation (PMID:21805466). Through this activity L-plastin builds and stabilizes lamellipodia, filopodia, and podosomes, sustains LFA-1 clustering and immunological synapse formation, and supports T, B, and macrophage migration and development, as defined by knockout, knockdown, and inhibitory-nanobody studies (PMID:21076065, PMID:20683899, PMID:21832165, PMID:27614263, PMID:19726756); it further promotes alveolar macrophage maturation and scaffolds an ASC–Pyk2 complex required for NLRP3 inflammasome assembly (PMID:27758872, PMID:35294888). L-plastin activity is reversibly inactivated by redox modification—an ROS-induced Cys101–Cys42 disulfide reversed by the Thioredoxin1 system, and S-glutathionylation at Cys460 in the actin-binding domain—both of which reduce bundling and impair cell motility and invasion (PMID:31501427, PMID:25881549). Ectopic expression and Ser5 phosphorylation of L-plastin in non-hematopoietic tumor cells drive invadopodia-mediated ECM degradation, invasion, and metastasis (PMID:17290393, PMID:24438191, PMID:26945069). Human LCP1 deficiency from a splice variant causes lymphopenia, neutropenia, and thrombocytopenia with defective actin organization and impaired immune cell migration, and an I232F missense mutation produces granulocytic dysplasia with aberrant nuclear mislocalization (PMID:38710235, PMID:34991157).

Mechanistic history

Synthesis pass · year-by-year structured walk · 24 steps
  1. 1992 High

    Established the core biochemical activity: that L-plastin is a calcium-regulated actin cross-linker with defined isoform and stoichiometric specificity, distinguishing it from related bundlers.

    Evidence In vitro F-actin bundling and co-sedimentation assays with purified human T-cell L-plastin under defined calcium conditions

    PMID:1491005 PMID:8461306

    Open questions at the time
    • Did not define how calcium is sensed structurally
    • Cellular triggers of bundling not addressed
  2. 1994 High

    Connected L-plastin biochemistry to receptor signaling by showing it associates with the cytoskeleton and participates in FcR-mediated calcium responses in intact neutrophils.

    Evidence Chemical labeling (BPB) in intact cells, immunoprecipitation, cytoskeletal fractionation, and Ca2+ flux measurements

    PMID:8170942

    Open questions at the time
    • Mechanism linking L-plastin to Ca2+ flux not defined
    • No specific receptor identified
  3. 1996 High

    Identified FcγRII as the upstream receptor and podosomes as a localization target, separating adhesion signaling from cytoskeletal rearrangement requirements.

    Evidence Receptor-blocking antibodies, LAD (CD18-null) patient cells, cytochalasin D, and immunofluorescence colocalization

    PMID:8663066

    Open questions at the time
    • Did not identify the kinase acting on L-plastin
    • Role of L-plastin within podosomes unresolved
  4. 1998 High

    Defined Ser5 phosphorylation as a sufficient molecular switch for integrin-mediated adhesion, shifting the model from passive bundler to actively regulated effector.

    Evidence Cell-permeable Ser5 and Ser5Ala peptides in primary human PMNs and monocytes with adhesion assays and PI3K/PKC inhibitors

    PMID:9689080

    Open questions at the time
    • Did not identify the physiological Ser5 kinase
    • Mechanism of integrin activation by the headpiece unresolved
  5. 1999 High

    Identified PKA as a Ser5 kinase acting downstream of PI3K and FcγR-induced cAMP, providing the first complete signaling chain to L-plastin activation.

    Evidence In vitro PKA kinase assay on L-plastin peptides, cAMP analogs/forskolin, PKA and PI3K inhibitors, cAMP measurement

    PMID:10446213

    Open questions at the time
    • Other receptors may use different kinases
    • Direct structural consequence of Ser5 phosphorylation not shown
  6. 2001 High

    Began mapping L-plastin's protein and integrin interactions, showing Ca2+-favored binding to grancalcin and headpiece-driven activation of αvβ3 conformational change.

    Evidence Affinity chromatography and reciprocal Co-IP (grancalcin); cell-permeable headpiece peptides with LIBS conformational antibodies in K562 cells

    PMID:11278342 PMID:11279160

    Open questions at the time
    • Functional role of grancalcin binding not established
    • Whether full-length protein recapitulates peptide effects unclear
  7. 2004 High

    Defined the actin-binding architecture—two ABDs of distinct affinity binding laterally along the filament—and identified phosphoinositides as inhibitors of actin binding.

    Evidence Recombinant domain expression, cross-linking, F-actin co-sedimentation, pyrenyl depolymerization, phosphoinositide competition; fMLP receptor pharmacology with MS

    PMID:14556648 PMID:14992580

    Open questions at the time
    • Atomic contact map of ABD-actin interface not resolved
    • Receptor-specific kinases for fMLP-induced phosphorylation not identified
  8. 2006 High

    Demonstrated directly that Ser5 phosphorylation increases F-actin binding and targets L-plastin to peripheral protrusions, linking the modification to invasive behavior.

    Evidence In vitro F-actin binding assay plus Ser5Ala/Ser5Glu phosphovariant transfection in Vero/HEK293T cells and 3D collagen invasion assay

    PMID:16636079

    Open questions at the time
    • Did not address phosphorylation kinetics in living cells
    • In vivo relevance not tested
  9. 2007 High

    Extended the phospho-switch to functional immune and tumor outputs—T cell surface receptor transport and melanoma invasion/metastasis—showing expression and phosphorylation have separable roles.

    Evidence MS phosphosite mapping with 5A-LPL mutagenesis in primary T cells (CD25/CD69); siRNA and phosphomutant expression with Matrigel invasion and in vivo B16 metastasis

    PMID:17290393 PMID:17294403

    Open questions at the time
    • Mechanism coupling L-plastin to surface receptor transport unresolved
    • Distinction between migration and invasion contributions incomplete
  10. 2009 Medium

    Causally tied L-plastin bundling activity to filopodia formation using domain-specific intracellular nanobodies, and linked Ser5 phosphorylation to TNF-α death resistance.

    Evidence Intracellular nanobody expression with filopodia and bundling readouts; phosphomutant overexpression with apoptosis assays and PKC inhibitors

    PMID:19726756 PMID:19799649

    Open questions at the time
    • Single-lab nanobody tools
    • Mechanism of death resistance not defined beyond phosphorylation dependence
  11. 2010 High

    Multiple knockout and quantitative studies established L-plastin as essential for T cell synapse formation, LFA-1 clustering, chemokine-driven migration, integrin binding, and osteoclast structures, and quantified its kinetic effect on actin turnover.

    Evidence LPL-/- mice with synapse imaging, CCR7 polarization, two-photon node motility, EAE/allograft models; FRAP with modeling, PKCδ siRNA, cortactin Co-IP; integrin domain mapping with calpain; osteoclast imaging; NES analysis

    PMID:15752138 PMID:20169155 PMID:20183869 PMID:20194718 PMID:20650888 PMID:20683899 PMID:21076065

    Open questions at the time
    • Integration of distinct kinase inputs across cell types not unified
    • Functional consequence of nucleocytoplasmic shuttling unclear
  12. 2011 High

    Defined L-plastin's roles in B cell development and eosinophil priming and identified cell-type-specific kinases (PKCβII) and signaling complexes (Pyk-2, GM-CSFRα, paxillin, cofilin).

    Evidence LPL-/- B cell migration and development assays with Pyk-2 phosphorylation; eosinophil phosphoproteomics, Co-IP, PKCβII siRNA, and synthetic phosphopeptide reconstitution

    PMID:21525390 PMID:21832165

    Open questions at the time
    • How distinct kinases are selected per receptor unresolved
    • Stoichiometry of the eosinophil complex undefined
  13. 2012 High

    Showed dexamethasone acts through suppression of L-plastin Ser5 phosphorylation, and that L-plastin organizes leading-edge actin and Rac1/Akt signaling during chemotaxis via PKCζ.

    Evidence Dexamethasone with phosphomimetic rescue in primary T cells; siRNA knockdown with Rac1 pulldown, Akt immunoblot, PKC isoform inhibitors, and nanobody conformational trapping

    PMID:21805466 PMID:22581862 PMID:23001012

    Open questions at the time
    • Direct kinase target of dexamethasone-regulated pathway not pinpointed
    • Order of Rac1/Akt relative to L-plastin not fully resolved
  14. 2013 Medium

    Established that L-plastin stabilizes podosomes and supports matrix degradation through its bundling activity independently of Ser5 phosphorylation status in macrophages.

    Evidence Intracellular nanobody expression with live podosome imaging, matrix degradation, and phospho-L-plastin immunostaining in THP-1 macrophages

    PMID:24236012

    Open questions at the time
    • Single-lab nanobody approach
    • Relationship between bundling and phosphorylation contributions at podosomes not reconciled
  15. 2014 High

    Demonstrated in vivo that both L-plastin expression and Ser5 phosphorylation are required for tumor growth and metastasis across prostate and melanoma models.

    Evidence shRNA knockdown and phosphorylatable vs. non-phosphorylatable ectopic expression with subcutaneous and intracardiac mouse metastasis models

    PMID:24438191

    Open questions at the time
    • Tumor-cell kinase driving Ser5 in vivo not identified here
    • Downstream invasion machinery not mapped
  16. 2015 High

    Identified redox modification—S-glutathionylation at Cys460 in the ABD—as an inactivating regulatory layer, and RSK1/2 as direct ERK/MAPK-driven Ser5 kinases in cancer.

    Evidence Cys460 mutagenesis with bundling and neutrophil functional assays and diabetic patient samples; in vitro RSK1/2 kinase assay with siRNA/shRNA and invasion assays

    PMID:25881549 PMID:26631483

    Open questions at the time
    • Enzymes catalyzing/reversing glutathionylation not defined
    • Interplay between phosphorylation and oxidation not addressed
  17. 2016 High

    Resolved distinct biophysical roles versus fascin in invadopodia/filopodia, identified Mst1 as a Thr89 kinase, and placed L-plastin in alveolar macrophage maturation and podosome longevity.

    Evidence Nanobody inhibition with EM of bundle ultrastructure; in vitro Mst1 kinase assay, Co-IP, T89A rescue in LPL-/- mice; conditional macrophage knockout with PPAR-γ analysis; live podosome imaging in LPL-/- macrophages

    PMID:26945069 PMID:27465533 PMID:27614263 PMID:27758872

    Open questions at the time
    • Functional role of Thr89 phosphorylation beyond localization unclear
    • How L-plastin couples to PPAR-γ upregulation unresolved
  18. 2017 High

    Provided a structural mechanism for calcium regulation by identifying a switch helix in the EF-hand headpiece that controls bundling activity.

    Evidence NMR solution structure of the EF-hand headpiece with competitive peptide inhibition of bundling

    PMID:28145401

    Open questions at the time
    • Structure of full-length protein bound to actin not determined
    • How Ser5 phosphorylation intersects the switch helix unresolved
  19. 2018 Medium

    Extended kinase inputs to LRRK1 in osteoclasts and characterized osteoclast bone-resorption roles via Ser5/Ser7-dependent sealing-zone formation.

    Evidence LC/MS phosphoproteomics and phospho-specific Western in Lrrk1-KO osteoclasts with micro-CT; TAT-peptide transduction with bone resorption assays

    PMID:30136304 PMID:30244178

    Open questions at the time
    • Whether LRRK1 phosphorylates L-plastin directly not shown
    • Single-lab peptide competition for bone resorption readout
  20. 2019 High

    Defined the Thioredoxin1-reversible Cys101–Cys42 disulfide as a localized redox switch and placed LCP1 upstream of mTORC2/AKT signaling in eosinophilic cells.

    Evidence TRX1 trapping/knockdown, auranofin, ratiometric LPL-roGFP-Orp1 imaging, proximity ligation, bundling and migration/invasion assays; shRNA knockdown with phospho-specific AKT/STAT immunoblots

    PMID:31501427 PMID:31691359

    Open questions at the time
    • Trigger localizing oxidation to cell extrusions unresolved
    • Mechanism linking LCP1 to mTORC2 activity undefined
  21. 2020 High

    Defined opposing osteoclast and megakaryocyte roles and identified upstream transcriptional (PI3K/AKT/SP1) and post-transcriptional (miR-125a-5p) control of LCP1 expression.

    Evidence LPL-KO mice with histomorphometry, filopodia/fusion and PDGF-BB assays, SP1 promoter studies; miR overexpression/knockdown with luciferase reporter and proplatelet formation quantification

    PMID:32844999 PMID:33208358

    Open questions at the time
    • How L-plastin negatively regulates the megakaryocyte invagination system unclear
    • Coupling of PI3K/AKT/SP1 to RANKL kinetics incomplete
  22. 2021 High

    Dissected spatial regulation of LFA-1 (Ser5-phosphorylation-dependent clustering via nPKC-MEK-p90RSK versus cofilin-mediated recruitment) and added the PI3K/SGK3 axis to cancer-cell Ser5 phosphorylation driving invadopodia and MMP-9-dependent degradation.

    Evidence Phospho-imaging, nPKC/MEK/p90RSK and PP2A inhibitors, phosphomutants, super-resolution microscopy; computational modeling with PI3K/SGK3 perturbation, kinase validation, and gelatin degradation/zymography

    PMID:33449151 PMID:33618712

    Open questions at the time
    • Integration of redundant Ser5 kinases in a single cell unresolved
    • PP2A site specificity not defined
  23. 2022 Medium

    Established L-plastin as an NLRP3 inflammasome scaffold via ASC-Pyk2 stabilization and linked human LCP1 mutations to defined hematologic/immunologic disease, including nuclear mislocalization of an I232F mutant.

    Evidence LPL-/- mice with ASC oligomerization, ASC-Pyk2-LPL Co-IP, caspase-1/IL-1β/gasdermin-D assays and bleomycin fibrosis; lentiviral I232F expression with fractionation and functional assays

    PMID:34991157 PMID:35294888

    Open questions at the time
    • Single-lab characterization of I232F mutant
    • How the inflammasome scaffolding role relates to actin bundling unresolved
  24. 2022 High

    Demonstrated that partial human L-plastin deficiency causes a multilineage cytopenia syndrome, defining a Mendelian disease link.

    Evidence Human kindred genetic analysis with orthologous mouse knock-in and Jurkat engineering, migration, flow cytometry, and platelet spreading assays

    PMID:38710235

    Open questions at the time
    • Genotype-phenotype spectrum across additional patients not established
    • Tissue-specific molecular basis of each cytopenia incomplete

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the many redundant and cell-type-specific Ser5 kinases, the Thr89 input, calcium switching, and redox modifications are integrated to set L-plastin bundling activity in a given physiological context remains unresolved.
  • No unified model coupling phosphorylation, calcium, and redox states
  • No full-length actin-bound structure
  • Quantitative hierarchy of kinase inputs per cell type unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 7 GO:0060090 molecular adaptor activity 3 GO:0098772 molecular function regulator activity 3
Localization
GO:0005856 cytoskeleton 6 GO:0005886 plasma membrane 3 GO:0005634 nucleus 2 GO:0005829 cytosol 2
Pathway
R-HSA-162582 Signal Transduction 6 R-HSA-1643685 Disease 5 R-HSA-168256 Immune System 5
Partners
ACTBCTTNGCAITGB1ITGB2PRKCBPTK2BPYCARD
Complex memberships
NLRP3 inflammasome (ASC-Pyk2-LPL scaffold)immunological synapseinvadopodiapodosome

Evidence

Reading pass · 47 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1992 L-plastin bundles F-actin in a strictly calcium-dependent manner: bundles form at pCa 7 but not at pCa 6, and approximately one molecule of L-plastin binds 8 actin monomers in the filament. Unlike fimbrin, it acts on beta-actin but not muscle alpha-actin or gizzard gamma-actin. In vitro F-actin bundling assay with purified human T-cell L-plastin under defined calcium conditions Journal of biochemistry High 1491005
1993 Macrophage 70-kDa actin-bundling protein (p70) is identical to L-plastin; its cross-linking activity is optimal at free calcium below 0.15 µM and is progressively inhibited at higher calcium (half-maximal inhibition at 1.6 µM). The protein undergoes major conformational changes at 0.15–1.5 µM free calcium and does not sever actin filaments. Protein purification, falling-ball viscosimetry, fluorescence spectroscopy, actin co-sedimentation assays Biochemistry High 8461306
1994 Bromophenacyl bromide (BPB) alkylates L-plastin in intact neutrophils and inhibits Fc receptor-mediated (IgG-stimulated), IP3-independent intracellular Ca2+ increase; L-plastin associates with the Triton X-100-insoluble cytoskeleton in adherent PMNs, linking it to FcR signal transduction. Chemical labeling with BPB in intact cells, immunoprecipitation with anti-BPB monoclonal antibody, cytoskeletal fractionation, Ca2+ flux measurements Proceedings of the National Academy of Sciences of the United States of America High 8170942
1996 FcγRII (not FcγRIIIB) is the primary receptor mediating L-plastin phosphorylation in neutrophils; adhesion to immune complexes is required but actin cytoskeleton rearrangement is not. L-plastin localizes to podosomes upon adhesion to immune complexes, colocalizing with actin, vinculin, and alpha-actinin. Receptor-specific monoclonal antibody blocking, patient cells with leukocyte adhesion deficiency (CD18-null), cytochalasin D treatment, immunofluorescence colocalization The Journal of biological chemistry High 8663066
1998 L-plastin phosphorylation on Ser5 is sufficient to induce leukocyte integrin-mediated adhesion: cell-permeable Ser5-containing peptides activate integrin-mediated adhesion in PMNs and monocytes, while Ser5→Ala substitution abolishes activity. Peptide-induced adhesion is sensitive to PI3K and PKC inhibitors, but a phosphoserine-5 peptide is insensitive. Cell-permeable peptide introduction into primary human PMNs and monocytes, adhesion assays, site-directed mutagenesis (Ser5Ala), pharmacological inhibitors of PI3K and PKC Proceedings of the National Academy of Sciences of the United States of America High 9689080
1999 PKA phosphorylates L-plastin on Ser5 in vitro and in cells downstream of PI3K and FcγR signaling: cAMP analogs and forskolin induce L-plastin phosphorylation; PKA inhibitors H89 and KT5720 block FcγR-induced L-plastin phosphorylation and integrin αMβ2 activation. FcγR stimulation transiently increases cAMP. PI3K acts upstream of PKA in this pathway. In vitro kinase assay (PKA + L-plastin peptides), cAMP analogs, adenylate cyclase activator, PKA inhibitors, PI3K inhibitors, cAMP measurement The Journal of biological chemistry High 10446213
2001 Grancalcin (penta-EF-hand Ca2+-binding protein) interacts with L-plastin in a Ca2+-dependent manner (interaction occurs in the absence of Ca2+, i.e., is favored at low Ca2+), identified by affinity chromatography of solubilized neutrophils and confirmed by reciprocal co-immunoprecipitation. Affinity chromatography on immobilized grancalcin, reciprocal co-immunoprecipitation, multiple independent methods The Journal of biological chemistry High 11279160
2001 L-plastin N-terminal peptides and headpiece domain activate αvβ3-mediated adhesion in K562 cells; this activation requires actin filament disassembly (jasplakinolide blocks it) and synergizes with RGD ligand or Mn2+ to induce the high-affinity integrin conformation (ligand-induced binding site epitope), independently of β3 cytoplasmic tail tyrosines. Cell-permeable peptides in K562 cells, LIBS antibody binding assay for integrin conformation, jasplakinolide and cytochalasin D treatment, adhesion assays The Journal of biological chemistry High 11278342
2004 L-plastin binds actin laterally along the filament axis via two actin-binding domains (ABD) that have different affinities for actin; actin peptides 112-125 and 360-372 participate in the interface. Phosphoinositides inhibit actin binding. The two ABDs show distinct modes of actin interaction. Recombinant domain expression, chemical cross-linking, F-actin co-sedimentation, pyrenyl F-actin depolymerization assays, phosphoinositide competition Biochemistry High 14992580
2004 Two fMLP receptor subtypes in neutrophils both phosphorylate L-plastin but use different signaling intermediates: low-affinity fMLP receptor phosphorylation requires PI3K, PLD, and PKC, whereas high-affinity fMLP receptor phosphorylation requires an Ro-31-8220-sensitive kinase but not PI3K, PLD, or classical PKC isoforms. 2D-IEF/PAGE, MALDI-TOF MS, pharmacological inhibitors of PI3K/PLD/PKC, receptor subtype discrimination with antagonistic peptides The Biochemical journal Medium 14556648
2005 L-plastin distributes between nucleus and cytoplasm because it harbors a less conserved nuclear export sequence (NES) lacking F21 of T-plastin; insertion of Phe at the equivalent position enhances L-plastin nuclear export, explaining its cytoplasmic/nuclear distribution. Leptomycin B treatment, NES deletion/mutation analysis, nuclear export reporter assays, fluorescence microscopy Traffic Medium 15752138
2006 Phosphorylation of L-plastin on Ser5 increases its F-actin-binding activity in vitro, promotes targeting to actin-rich peripheral membrane protrusions and microspikes in cells, and is required for collagen invasion. Ser5Ala reduces association with F-actin; Ser5Glu (phosphomimetic) promotes F-actin microspike formation and remains detergent-resistant with F-actin. In vitro F-actin binding/co-sedimentation assay, transfection of phosphovariants (Ser5Ala, Ser5Glu) in Vero and HEK293T cells, detergent extraction, 3D collagen invasion assay Journal of cell science High 16636079
2007 Phosphorylation of L-plastin at Ser5 (identified by mass spectrometry) in T cells is required for surface transport of CD25 and CD69 to the T cell surface upon costimulation (TCR/CD3 + CD2 or CD28), but not for relocalization of L-plastin itself to the immunological synapse. Mass spectrometry phosphosite identification, site-directed mutagenesis (5A-LPL), retroviral transduction in primary human PBT, flow cytometry for CD25/CD69 surface expression European journal of immunology High 17294403
2007 Phosphorylation of L-plastin (on Ser5) in melanoma cells (but not expression alone) is required for enhanced Matrigel invasion; haptotactic migration (but not chemotactic migration or static adhesion) is enhanced by L-plastin expression regardless of phosphorylation status. In vivo metastatic capacity of B16 cells correlates with L-plastin expression and phosphorylation. siRNA knockdown, retroviral expression of wt-LPL-EGFP vs. nonphosphorylatable 5A7A-LPL-EGFP, Matrigel invasion/migration assays, in vivo mouse metastasis model International journal of cancer High 17290393
2009 Alpaca single-domain antibody (nanobody) directed against L-plastin blocks filopodia formation by obstructing L-plastin-mediated F-actin bundling, establishing a direct causal link between L-plastin bundling activity and filopodia formation. Recombinant nanobody expression in cells, F-actin bundling inhibition, filopodia formation assay FASEB journal Medium 19726756
2009 L-plastin confers resistance to TNF-α-induced cell death in MCF-7 breast cancer cells in a Ser5 phosphorylation-dependent manner; non-conventional PKC isoforms and the ceramide pathway regulate this phosphorylation state. siRNA knockdown, overexpression of wild-type and phosphomutant L-plastin, cell viability/apoptosis assays, PKC inhibitor studies Journal of cellular and molecular medicine Medium 19799649
2010 L-plastin (LPL) is required for TCR-mediated T cell activation: LPL-/- T cells show defective lamellipodia formation, smaller immunological synapses, impaired cytokine production, and reduced proliferation. LPL-/- mice show delayed allograft rejection and reduced EAE severity. LPL-/- mouse model, T cell spreading assays on immobilized TCR ligands, immunological synapse imaging with APCs, cytokine ELISA, allograft rejection model, EAE model Journal of immunology High 21076065
2010 Sustained LFA-1 (but not TCR/CD3) clustering in the peripheral SMAC of the immunological synapse requires L-plastin; calmodulin binding to L-plastin is required for maintenance of L-plastin in the IS, and calmodulin inhibition prevents stable LFA-1 and Talin redistribution. siRNA knockdown of L-plastin, IS immunofluorescence imaging (LFA-1, Talin, CD3 redistribution), calmodulin inhibitor treatment, T cell proliferation assay European journal of immunology High 20683899
2010 L-plastin is required for CCR7-mediated T cell motility and cellular polarization (CCR7 fails to polarize to leading edge in LPL-/- T cells) and for thymic egress, but is dispensable for CCR7 proximal signaling to F-actin polymerization and CCR7-mediated costimulation. LPL-/- mice, CCR7-stimulated T cell polarization assays, two-photon microscopy of lymph node motility, thymic egress quantification, F-actin polymerization assay Journal of immunology High 20194718
2010 In focal adhesions, L-plastin undergoes rapid association/dissociation cycles following a two-binding-state model; Ser5 phosphorylation increases association rates 2-fold without affecting dissociation rates, and L-plastin decreases actin dissociation rate 4-fold thereby increasing F-actin content. PKC-delta mediates PMA-induced L-plastin Ser5 phosphorylation. L-plastin associates with a complex containing cortactin. FRAP of GFP-coupled L-plastin phosphovariants in live cells, mathematical modeling, siRNA knockdown of PKC isoforms, co-immunoprecipitation with cortactin PloS one High 20169155
2010 L-plastin interacts with the cytoplasmic domain of β1 and β2 integrins via its two actin-binding domains (not the N-terminal headpiece); this complex is regulated by µ-calpain, which cleaves β-integrin and disrupts the L-plastin/integrin interaction but does not directly dissociate it via calcium. Co-immunoprecipitation of endogenous proteins, solid phase assays, pull-down with recombinant L-plastin domains, cross-linking, ELISA, µ-calpain cleavage experiments Cytoskeleton High 20183869
2010 In osteoclasts, L-plastin is present in actin aggregates during the early phase of sealing ring formation; cortactin is involved in the maturation phase. Temporal changes in L-plastin localization (from actin aggregates) and cortactin localization (in sealing ring) indicate sequential roles during bone resorption. siRNA to cortactin, immunofluorescence, quantification of L-plastin and cortactin localization during RANKL/M-CSF-induced bone resorption The Journal of biological chemistry Medium 20650888
2011 L-plastin is required for marginal zone B cell development and B cell motility toward CXCL12, CXCL13, and S1P; LPL-/- B cells fail to enter lymph nodes and bone marrow. LPL is required for integrin-mediated enhancement of Transwell migration but not for integrin-mediated adhesion per se. LPL-/- B cells show reduced Pyk-2 phosphorylation in response to chemokine. LPL-/- mouse model, Transwell migration assays, flow cytometry of splenic B cell subsets, integrin adhesion assays, Pyk-2 phosphorylation by immunoblot Journal of immunology High 21832165
2011 GM-CSF priming of eosinophils for chemotaxis and degranulation is mediated by PKCβII-phosphorylated L-plastin; co-immunoprecipitation reveals a complex of phospho-L-plastin with PKCβII, GM-CSF receptor α-chain, paxillin, and cofilin. A synthetic Ser5-phosphorylated L-plastin peptide (residues 2-19) recapitulates GM-CSF effects on integrin αMβ2 and chemotaxis independently of GM-CSF. Phosphoproteomics, co-immunoprecipitation, PKCβII siRNA and inhibitors, synthetic phosphopeptide stimulation, flow cytometry, chemotaxis and degranulation assays Journal of immunology High 21525390
2011 Dexamethasone inhibits costimulation-induced L-plastin Ser5 phosphorylation in primary human T cells, thereby reducing F-actin accumulation and immune synapse maturation; expression of a phosphomimetic L-plastin reverses the immunosuppressive effect of dexamethasone. Dexamethasone treatment, expression of nonphosphorylatable and phosphomimetic L-plastin mutants in primary human PBT, F-actin staining, IS imaging, flow cytometry European journal of immunology High 21805466
2012 L-plastin is required for SDF-1α-stimulated T lymphocyte polarization, lamellipodia formation, and chemotaxis; knockdown impairs F-actin localization at the leading edge, Rac1 activation cycle, and Akt phosphorylation. PKCζ (not other PKC isoforms) regulates L-plastin Ser5 phosphorylation in response to SDF-1α, and L-plastin colocalizes with PKCζ at the leading edge. siRNA knockdown, immunofluorescence colocalization, PKC isoform-selective inhibitors, Rac1 GTPase pulldown, Akt immunoblot, chemotaxis and polarization assays Journal of immunology High 22581862
2012 Nanobodies targeting either the EF-hand or actin-binding domains of L-plastin trap it in an inactive conformation, impairing IS formation, MTOC docking, T cell proliferation, IL-2 secretion, and delaying Ser5 phosphorylation. One nanobody also delays LFA-1 phosphorylation and reduces L-plastin–LFA-1 association. Intracellular nanobody expression in Jurkat and PBMCs, IS imaging, MTOC docking assay, IL-2 ELISA, T cell proliferation, co-immunoprecipitation of L-plastin with LFA-1 Cellular and molecular life sciences High 23001012
2013 L-plastin in THP-1 macrophages is required for podosome formation and stability, and for matrix degradation; inhibitory nanobodies cause irregular, unstable actin turnover at podosomes without affecting Ser5 phosphorylation. Phosphorylated L-plastin is highly enriched in podosomes. Intracellular nanobody expression, live-cell imaging of podosome dynamics, matrix degradation assay, phospho-L-plastin immunostaining PloS one Medium 24236012
2014 L-plastin expression and its phosphorylation are both required in vivo for tumor metastasis: L-plastin knockdown in PC3M prostate cancer cells reduces tumor growth and metastasis; ectopic expression of phosphorylatable but not non-phosphorylatable L-plastin in MV3 melanoma cells increases metastasis in mouse preclinical models. shRNA knockdown, ectopic expression of phosphorylatable vs. non-phosphorylatable L-plastin, subcutaneous and intracardiac injection mouse models, micro-CT/histomorphometry Molecular cancer High 24438191
2015 S-glutathionylation of L-plastin at Cys206, Cys283, and Cys460 (Cys460 being most critical) in the actin-binding domain reduces its actin-binding and bundling activity, impairing neutrophil chemotaxis, polarization, bactericidal activity, and phagocytosis. S-thiolated L-plastin is detected in diabetic patient neutrophils. In vitro and cell-based oxidative stress, site-directed mutagenesis (Cys460), actin co-sedimentation assay, neutrophil functional assays, clinical samples Free radical biology & medicine High 25881549
2015 RSK1 and RSK2, downstream of the ERK/MAPK pathway, directly phosphorylate L-plastin on Ser5 in vitro. RSK is an essential activator of L-plastin in breast cancer cells; RSK knockdown reduces migration and invasion and impairs Ser5-phospho-L-plastin recruitment to migratory structures. In vitro kinase assay (RSK1/2 + L-plastin), siRNA/shRNA knockdown, invasion and migration assays, immunofluorescence, computational modeling validated experimentally FASEB journal High 26631483
2016 L-plastin and fascin cooperate in invadopodia and filopodia with distinct roles: L-plastin bundles are thinner and less tightly packed than fascin bundles, providing flexibility for elongation while fascin provides rigidity. L-plastin is identified as a component of invadopodia contributing to matrix degradation and invasiveness; specific nanobodies inhibiting L-plastin bundling cannot be compensated by fascin. Domain-specific nanobody inhibition of bundling, invadopodia matrix degradation assay, electron microscopy of bundle ultrastructure, protrusion quantification The Journal of biological chemistry High 26945069
2016 Mst1 kinase phosphorylates L-plastin on Thr89 in vitro and interacts with L-plastin in cells; T89A mutation impairs L-plastin localization to lamellipodia and fails to restore T cell migration and thymic egress in LPL-/- mice, establishing LPL as a downstream effector of Mst1. In vitro kinase assay (Mst1 + LPL), co-immunoprecipitation in cells, Thr89Ala mutagenesis, bone marrow chimeras, T cell migration/polarization assays, thymic egress quantification Journal of immunology High 27465533
2016 LPL is required for the transition from prealveolar macrophages to mature alveolar macrophages; LPL-/- precursors fail to upregulate PPAR-γ despite abundant GM-CSF signaling, suggesting LPL supports transmigration into and engraftment in alveoli where GM-CSF is produced. Conditional knockout (CD11c.Cre-LPLfl/fl), flow cytometry of alveolar macrophage subsets, GM-CSF signaling analysis, irradiation-regeneration assay, pneumococcal infection challenge Blood High 27758872
2016 L-plastin promotes podosome longevity (but not initiation) in primary resident peritoneal macrophages; loss of LPL leads to decreased podosome lifetime, failure to elongate in response to chemotactic stimulation, and impaired macrophage transmigration and peritoneal migration. Live-cell imaging of LifeACT-RFP macrophages from LPL-/- mice, podosome longevity quantification, macrophage transmigration assay, in vivo peritoneal migration Molecular immunology High 27614263
2017 NMR structure of the L-plastin EF-hand headpiece reveals a novel switch helix immediately after the calcium-binding region that binds tightly to the EF-hand motifs in the presence of calcium. This switch helix plays a major role in actin-bundling regulation; a competitive peptide inhibiting EF-hand/switch helix association deregulates bundling activity. NMR spectroscopy (solution structure determination), competitive peptide inhibition of actin-bundling activity Scientific reports High 28145401
2018 LRRK1 signaling in osteoclasts promotes L-plastin Ser5 phosphorylation; Lrrk1-knockout osteoclasts show reduced L-plastin Ser5 phosphorylation by LC/MS and Western blot. L-plastin KO mice show increased trabecular bone volume. Metal affinity purification LC/MS phosphoproteomics, Ser5-specific Western blot, micro-CT of L-plastin KO mice Journal of cellular biochemistry Medium 30136304
2018 TNF-α signaling phosphorylates L-plastin on Ser5 and Ser7 in osteoclasts, increasing actin bundling capacity and driving formation of nascent sealing zones (NSZs). TAT-fused full-length L-plastin peptide increases NSZ/sealing ring formation; N-terminal phospho-Ser peptides reduce cellular L-plastin phosphorylation and bone resorption. Neither podosome assembly nor osteoclast migration is affected. TAT-peptide transduction, immunoblotting with phospho-specific antibodies, actin staining, bone resorption (dentine pit) assay Experimental cell research Medium 30244178
2019 L-plastin is reversibly regulated by ROS-induced thiol oxidation: Cys101 forms a disulfide bridge with Cys42, reducing actin-bundling capacity. Reduction is mediated by the Thioredoxin1 (TRX1) system (demonstrated by TRX1 trapping and TRXR1 blockade with auranofin). L-plastin oxidation occurs preferentially in actin-based cell extrusions and inhibits cell spreading, filopodial extension, migration, invasion, and ECM degradation. TRX1 trapping (substrate trap), TRX1 knockdown, TRXR1 inhibitor (auranofin), ratiometric imaging (LPL-roGFP-Orp1 fusion), dimedone-based proximity ligation assay, actin-bundling assay, functional migration/invasion/ECM assays Nature communications High 31501427
2019 LCP1 knockdown in Eol-1 eosinophilic cells reduces phosphorylation of AKTSer473 (mTORC2-dependent) and STAT1Tyr701 but not FIP1L1-PDGFRA-induced STAT3/STAT5/ERK phosphorylation, establishing LCP1 as upstream of mTORC2/AKT in eosinophilic cells. shRNA knockdown of LCP1, PKCβ inhibitor enzastaurin, phospho-specific immunoblot for mTORC2/AKT pathway components, eosinophil differentiation and apoptosis assays Molecular carcinogenesis Medium 31691359
2020 LPL deletion in osteoclasts impedes preosteoclast fusion by inhibiting filopodia formation, increases preosteoclast numbers (which release PDGF-BB to promote vessel growth and bone formation), and LPL expression is regulated by the PI3K/AKT/SP1 axis in response to RANKL. LPL knockout mice, histomorphometry, flow cytometry, filopodia imaging, PDGF-BB secretion assay, PI3K/AKT inhibition/SP1 promoter studies Science advances High 33208358
2020 miR-125a-5p directly targets and reduces L-plastin (LCP1) expression in megakaryocytes; L-plastin promotes megakaryocyte progenitor migration but negatively regulates proplatelet formation (PPF) and PP branching by inhibiting the late-stage megakaryocyte invagination system and podosomes. miR overexpression/knockdown, LCP1 knockdown and overexpression in MKs, in vivo miR inhibition (platelet count), luciferase reporter assay (miR-125a-5p targeting LCP1 3'UTR), PPF quantification Blood High 32844999
2021 LFA-1 cluster formation (avidity regulation) in T cells depends on L-plastin Ser5 phosphorylation mediated by an nPKC-MEK-p90RSK pathway and counter-regulated by PP2A. Ser5 phosphorylation is required for LFA-1 clustering in high-affinity conformation; cofilin dephosphorylation (not LPL phosphorylation) mediates LFA-1 recruitment to the T-cell/APC contact zone—a distinct, dichotomic spatial regulation. Phospho-specific imaging, nPKC/MEK/p90RSK and PP2A inhibitors, phosphomutant expression, super-resolution microscopy of LFA-1 nanoclusters Cellular and molecular life sciences High 33449151
2021 PI3K/SGK3 pathway contributes to L-plastin Ser5 phosphorylation in breast cancer cells (in addition to ERK/MAPK-RSK), shown by computational modeling and experimental validation. Ser5 phosphorylation promotes L-plastin recruitment to invadopodia, MMP-9 activity, and ECM degradation. Computational network modeling, PI3K/SGK3 inhibition/overexpression, in vitro kinase validation, shRNA knockdown, invasion/migration assays, confocal imaging, gelatin degradation/zymography Cell communication and signaling Medium 33618712
2022 LCP1 I232F missense mutation causes granulocytic dysplasia, reduced proliferation, G2/M cell cycle arrest, impaired cell motility/invasiveness, increased F-actin, and aberrant nuclear localization of the mutant protein (wild-type L-plastin is cytoplasmic). The mutation does not block differentiation or increase apoptosis. Lentiviral inducible expression of LCP1 I232F in 32D and HeLa cells, flow cytometry (cell cycle, F-actin), confocal imaging, subcellular fractionation, motility/invasion assays Blood advances Medium 34991157
2022 L-plastin (LPL) enhances NLRP3 inflammasome assembly by stabilizing ASC interactions with Pyk2 (a component of podosomes), enabling ASC oligomerization. LPL-/- alveolar macrophages exhibit reduced caspase-1 activity, IL-1β cleavage, and gasdermin-D processing upon NLRP3 activation; LPL-/- mice are resistant to bleomycin-induced lung fibrosis. LPL-/- mouse model, ASC oligomerization assay, co-immunoprecipitation (ASC-Pyk2-LPL), caspase-1 activity and IL-1β processing assays, bleomycin lung fibrosis model Cell reports High 35294888
2022 LCP1 splicing variant (c.740-1G>A) causes partial L-plastin deficiency resulting in lymphopenia, neutropenia, and thrombocytopenia. Functional analysis shows defective actin organization in T cells, reduced PBMC migration in response to CXCL12, impaired germinal center B-cell expansion, reduced T-cell cytokinesis, and aberrant platelet spreading on collagen. Human kindred genetic analysis, orthologous mouse knock-in, Jurkat cell mutation engineering, migration assays, flow cytometry, platelet spreading assay The Journal of allergy and clinical immunology High 38710235

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1998 A role for the actin-bundling protein L-plastin in the regulation of leukocyte integrin function. Proceedings of the National Academy of Sciences of the United States of America 138 9689080
1992 Human T cell L-plastin bundles actin filaments in a calcium-dependent manner. Journal of biochemistry 131 1491005
2006 Phosphorylation on Ser5 increases the F-actin-binding activity of L-plastin and promotes its targeting to sites of actin assembly in cells. Journal of cell science 95 16636079
2011 The actin-bundling protein L-plastin: a critical regulator of immune cell function. International journal of cell biology 90 22194750
2007 Costimulation induced phosphorylation of L-plastin facilitates surface transport of the T cell activation molecules CD69 and CD25. European journal of immunology 89 17294403
2020 Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss. Science advances 81 33208358
2001 Differential expression of the L-plastin gene in human colorectal cancer progression and metastasis. Biochemical and biophysical research communications 79 11735128
2022 Inhibition of the Glycolysis Prevents the Cerebral Infarction Progression Through Decreasing the Lactylation Levels of LCP1. Molecular biotechnology 73 36574182
2006 The leukocyte protein L-plastin induces proliferation, invasion and loss of E-cadherin expression in colon cancer cells. International journal of cancer 72 16287074
2018 Exosomal Release of L-Plastin by Breast Cancer Cells Facilitates Metastatic Bone Osteolysis. Translational oncology 71 30583289
2012 L-plastin regulates polarization and migration in chemokine-stimulated human T lymphocytes. Journal of immunology (Baltimore, Md. : 1950) 64 22581862
2010 Actin-bundling protein L-plastin regulates T cell activation. Journal of immunology (Baltimore, Md. : 1950) 62 21076065
2010 Sustained LFA-1 cluster formation in the immune synapse requires the combined activities of L-plastin and calmodulin. European journal of immunology 61 20683899
2020 Exosomal Transfer of LCP1 Promotes Osteosarcoma Cell Tumorigenesis and Metastasis by Activating the JAK2/STAT3 Signaling Pathway. Molecular therapy. Nucleic acids 60 32810692
1994 Bromophenacyl bromide binding to the actin-bundling protein l-plastin inhibits inositol trisphosphate-independent increase in Ca2+ in human neutrophils. Proceedings of the National Academy of Sciences of the United States of America 58 8170942
1993 Characterization of the human L-plastin gene promoter in normal and neoplastic cells. The Journal of biological chemistry 57 8428953
2013 The actin-bundling protein L-plastin supports T-cell motility and activation. Immunological reviews 56 24117812
1996 FcgammaRII-mediated adhesion and phagocytosis induce L-plastin phosphorylation in human neutrophils. The Journal of biological chemistry 56 8663066
1999 Immune complex-induced integrin activation and L-plastin phosphorylation require protein kinase A. The Journal of biological chemistry 49 10446213
2016 Fascin Rigidity and L-plastin Flexibility Cooperate in Cancer Cell Invadopodia and Filopodia. The Journal of biological chemistry 48 26945069
2016 Lcp1 Is a Phosphotransferase Responsible for Ligating Arabinogalactan to Peptidoglycan in Mycobacterium tuberculosis. mBio 48 27486192
2010 Regulation of sealing ring formation by L-plastin and cortactin in osteoclasts. The Journal of biological chemistry 48 20650888
2007 Phosphorylation of ectopically expressed L-plastin enhances invasiveness of human melanoma cells. International journal of cancer 47 17290393
2019 Silencing the lncRNA Maclpil in pro-inflammatory macrophages attenuates acute experimental ischemic stroke via LCP1 in mice. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 45 30895879
2001 Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. The Journal of biological chemistry 45 11279160
2022 L-plastin enhances NLRP3 inflammasome assembly and bleomycin-induced lung fibrosis. Cell reports 43 35294888
2016 Alveolar macrophage development in mice requires L-plastin for cellular localization in alveoli. Blood 43 27758872
2010 The actin-bundling protein L-plastin dissociates CCR7 proximal signaling from CCR7-induced motility. Journal of immunology (Baltimore, Md. : 1950) 43 20194718
2009 An alpaca single-domain antibody blocks filopodia formation by obstructing L-plastin-mediated F-actin bundling. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 43 19726756
2003 Cytotoxic effect of replication-competent adenoviral vectors carrying L-plastin promoter regulated E1A and cytosine deaminase genes in cancers of the breast, ovary and colon. Cancer gene therapy 42 12719708
2011 The actin-bundling protein L-plastin is essential for marginal zone B cell development. Journal of immunology (Baltimore, Md. : 1950) 41 21832165
1999 Suppression of prostate carcinoma cell invasion by expression of antisense L-plastin gene. The American journal of pathology 41 10393844
1993 Purification and further characterization of macrophage 70-kDa protein, a calcium-regulated, actin-binding protein identical to L-plastin. Biochemistry 41 8461306
2020 PL-S2, a homogeneous polysaccharide from Radix Puerariae lobatae, attenuates hyperlipidemia via farnesoid X receptor (FXR) pathway-modulated bile acid metabolism. International journal of biological macromolecules 40 33058986
2014 Metastasis of prostate cancer and melanoma cells in a preclinical in vivo mouse model is enhanced by L-plastin expression and phosphorylation. Molecular cancer 40 24438191
2011 Priming of eosinophils by GM-CSF is mediated by protein kinase CbetaII-phosphorylated L-plastin. Journal of immunology (Baltimore, Md. : 1950) 39 21525390
2017 AP4 modulated by the PI3K/AKT pathway promotes prostate cancer proliferation and metastasis of prostate cancer via upregulating L-plastin. Cell death & disease 37 28981098
2012 Nanobody-induced perturbation of LFA-1/L-plastin phosphorylation impairs MTOC docking, immune synapse formation and T cell activation. Cellular and molecular life sciences : CMLS 37 23001012
2010 Quantitative kinetic study of the actin-bundling protein L-plastin and of its impact on actin turn-over. PloS one 36 20169155
2011 L-plastin phosphorylation: a novel target for the immunosuppressive drug dexamethasone in primary human T cells. European journal of immunology 35 21805466
2001 L-plastin peptide activation of alpha(v)beta(3)-mediated adhesion requires integrin conformational change and actin filament disassembly. The Journal of biological chemistry 35 11278342
2008 Pmp-like proteins Pls1 and Pls2 are secreted into the lumen of the Chlamydia trachomatis inclusion. Infection and immunity 34 18591235
2000 Upregulation of L-plastin gene by testosterone in breast and prostate cancer cells: identification of three cooperative androgen receptor-binding sequences. DNA and cell biology 34 10668786
2009 The actin filament cross-linker L-plastin confers resistance to TNF-alpha in MCF-7 breast cancer cells in a phosphorylation-dependent manner. Journal of cellular and molecular medicine 33 19799649
1997 Steroid hormone induction and expression patterns of L-plastin in normal and carcinomatous prostate tissues. The American journal of pathology 33 9176394
2017 The Calcium-Dependent Switch Helix of L-Plastin Regulates Actin Bundling. Scientific reports 32 28145401
2020 miR-125a-5p regulates megakaryocyte proplatelet formation via the actin-bundling protein L-plastin. Blood 31 32844999
2016 Mst1 Kinase Regulates the Actin-Bundling Protein L-Plastin To Promote T Cell Migration. Journal of immunology (Baltimore, Md. : 1950) 31 27465533
2004 N-Formyl peptide receptor subtypes in human neutrophils activate L-plastin phosphorylation through different signal transduction intermediates. The Biochemical journal 31 14556648
2013 L-plastin nanobodies perturb matrix degradation, podosome formation, stability and lifetime in THP-1 macrophages. PloS one 30 24236012
2018 Targeted deletion of the zebrafish actin-bundling protein L-plastin (lcp1). PloS one 28 29293625
2016 L-Plastin promotes podosome longevity and supports macrophage motility. Molecular immunology 28 27614263
2015 L-plastin Ser5 phosphorylation in breast cancer cells and in vitro is mediated by RSK downstream of the ERK/MAPK pathway. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 28 26631483
2015 L-Plastin S-glutathionylation promotes reduced binding to β-actin and affects neutrophil functions. Free radical biology & medicine 27 25881549
2000 Expression patterns of L-plastin isoform in normal and carcinomatous breast tissues. Anticancer research 27 11062740
2019 Spatial oxidation of L-plastin downmodulates actin-based functions of tumor cells. Nature communications 25 31501427
2016 L-plastin regulates the stability of the immune synapse of naive and effector T-cells. Advances in biological regulation 24 27720134
2004 Biochemical characterization of the L-plastin-actin interaction shows a resemblance with that of alpha-actinin and allows a distinction to be made between the two actin-binding domains of the molecule. Biochemistry 24 14992580
1994 Identification of the 65-kDa phosphoprotein in murine macrophages as a novel protein: homology with human L-plastin. Biochemical and biophysical research communications 24 8060349
2014 L-plastin is essential for alveolar macrophage production and control of pulmonary pneumococcal infection. Infection and immunity 23 24595139
1999 Nonrandom fusion of L-plastin(LCP1) and LAZ3(BCL6) genes by t(3;13)(q27;q14) chromosome translocation in two cases of B-cell non-Hodgkin lymphoma. Genes, chromosomes & cancer 22 10469447
2024 LCP1 knockdown in monocyte-derived macrophages: mitigating ischemic brain injury and shaping immune cell signaling and metabolism. Theranostics 21 38164159
2022 Efficient T Cell Migration and Activation Require L-Plastin. Frontiers in immunology 20 35844504
2013 Intrinsic T- and B-cell defects impair T-cell-dependent antibody responses in mice lacking the actin-bundling protein L-plastin. European journal of immunology 20 23589339
2008 Identification of L-plastin autoantibody in plasma of patients with non-Hodgkin's lymphoma using a proteomics-based analysis. Annals of clinical biochemistry 20 18275676
2018 Peptidomimetic inhibitors of L-plastin reduce the resorptive activity of osteoclast but not the bone forming activity of osteoblasts in vitro. PloS one 19 30248139
2018 Increased expression of L-plastin in nasal polyp of patients with nonsteroidal anti-inflammatory drug-exacerbated respiratory disease. Allergy 19 30479022
2022 Lymphocyte cytosolic protein 1 (L-plastin) I232F mutation impairs granulocytic proliferation and causes neutropenia. Blood advances 17 34991157
2010 Characterization of L-plastin interaction with beta integrin and its regulation by micro-calpain. Cytoskeleton (Hoboken, N.J.) 17 20183869
2009 Langerhans cell protein 1 (LCP1) binds to PNUTS in the nucleus: implications for this complex in transcriptional regulation. Experimental & molecular medicine 17 19293638
2016 Thymic stromal lymphopoietin regulates eosinophil migration via phosphorylation of l-plastin in atopic dermatitis. Experimental dermatology 16 27304220
2014 The actin-bundling protein L-plastin: a novel local inflammatory marker associated with periodontitis. Journal of periodontal research 15 25040533
1985 A case report of a patient with retinoblastoma and chromosome 13q deletion: assignment of a new gene (gene for LCP1) on human chromosome 13. Human genetics 15 3864729
2018 L-plastin phosphorylation regulates the early phase of sealing ring formation by actin bundling process in mouse osteoclasts. Experimental cell research 14 30244178
2014 Matrigel induces L-plastin expression and promotes L-plastin-dependent invasion in human cholangiocarcinoma cells. Oncology letters 14 25120647
2021 L-plastin Ser5 phosphorylation is modulated by the PI3K/SGK pathway and promotes breast cancer cell invasiveness. Cell communication and signaling : CCS 13 33618712
2019 LCP1 triggers mTORC2/AKT activity and is pharmacologically targeted by enzastaurin in hypereosinophilia. Molecular carcinogenesis 13 31691359
2007 Conformational analysis of the leukocyte-specific EF-hand protein p65/L-plastin by X-ray scattering in solution. Biophysical chemistry 13 17900788
2005 Molecular basis for dissimilar nuclear trafficking of the actin-bundling protein isoforms T- and L-plastin. Traffic (Copenhagen, Denmark) 13 15752138
2018 LRRK1 regulation of actin assembly in osteoclasts involves serine 5 phosphorylation of L-plastin. Journal of cellular biochemistry 12 30136304
2017 miR-375 Regulates Invasion-Related Proteins Vimentin and L-Plastin. The American journal of pathology 12 28499703
2011 Expression and clinical significance of L-plastin in colorectal carcinoma. Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract 12 21922341
1997 The murine L-plastin gene promoter: identification and comparison with the human L-plastin gene promoter. DNA and cell biology 12 9022040
1996 Characterization of the M(r) 65,000 lymphokine-activated killer proteins phosphorylated after tumor target binding: evidence that pp65a and pp65b are phosphorylated forms of L-plastin. Cancer research 12 8548753
2024 Tetrandrine alleviates inflammation and promotes macrophage M2 polarization in gouty arthritis by NF-κB-mediated Lcp1. Cellular and molecular biology (Noisy-le-Grand, France) 11 38430024
2021 Loss of lymphocyte cytosolic protein 1 (LCP1) induces browning in 3T3-L1 adipocytes via β3-AR and the ERK-independent signaling pathway. The international journal of biochemistry & cell biology 11 34371171
2020 The actin-bundling protein L-plastin-A double-edged sword: Beneficial for the immune response, maleficent in cancer. International review of cell and molecular biology 11 32859369
2014 L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration. Journal of leukocyte biology 11 24803550
2014 Potential diagnostic significance of HSP90, ACS/TMS1, and L-plastin in the identification of melanoma. Melanoma research 11 25191796
2003 Preparation and characterization of recombinant murine p65/L-plastin expressed in Escherichia coli and high-titer antibodies against the protein. Bioscience, biotechnology, and biochemistry 11 12843666
1985 Evidence for the close linkage between lymphocyte cytosol polypeptide with molecular weight of 64,000 (LCP1) and esterase D. American journal of human genetics 10 4083280
2021 LFA-1 cluster formation in T-cells depends on L-plastin phosphorylation regulated by P90RSK and PP2A. Cellular and molecular life sciences : CMLS 9 33449151
2018 Inhibition of HIF1α-Dependent Upregulation of Phospho-l-Plastin Resensitizes Multiple Myeloma Cells to Frontline Therapy. International journal of molecular sciences 9 29882856
2024 L-plastin associated syndrome of immune deficiency and hematologic cytopenia. The Journal of allergy and clinical immunology 8 38710235
2022 Follow that cell: Leukocyte migration in L-plastin mutant zebrafish. Cytoskeleton (Hoboken, N.J.) 8 35811499
2021 L-Plastin Promotes Gastric Cancer Growth and Metastasis in a Helicobacter pylori cagA-ERK-SP1-Dependent Manner. Molecular cancer research : MCR 8 33771880
2020 Neutrophil L-Plastin Controls Ocular Paucibacteriality and Susceptibility to Keratitis. Frontiers in immunology 8 32318063
2019 Dual inhibition of ABCE1 and LCP1 by microRNA-96 results in an additive effect in breast cancer mouse model. Oncotarget 8 31007850
2019 Engineering of L-Plastin Peptide-Loaded Biodegradable Nanoparticles for Sustained Delivery and Suppression of Osteoclast Function In Vitro. International journal of cell biology 8 31191656
2006 Analysis of a bacterial lipopolysaccharide-activated serine kinase that phosphorylates p65/L-plastin in macrophages. Microbiology and immunology 8 16625055

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