Affinage

KATNBL1

KATNB1-like protein 1 · UniProt Q9H079

Round 2 corrected
Length
304 aa
Mass
34.8 kDa
Annotated
2026-04-28
31 papers in source corpus 2 papers cited in narrative 5 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

KATNBL1 (also known as KIAA0556) is a regulatory subunit of the katanin microtubule-severing complex that associates with the catalytic A subunits KATNA1 and KATNAL1 and directly modulates their microtubule-severing activity in vitro (PMID:26929214). It competes with the canonical regulatory subunit KATNB1 (p80) for binding to these catalytic subunits and is sequestered in the nucleus during interphase via an N-terminal nuclear localization signal, relocalizing to spindle poles exclusively during mitosis (PMID:26929214). KATNBL1 localizes to the ciliary basal body, binds microtubules directly, and is required for normal ciliogenesis and ciliary microtubule architecture, as demonstrated by reduced and structurally abnormal cilia in patient-derived fibroblasts carrying null mutations and by ciliary A-tubule defects in C. elegans mutants (PMID:26714646).

Mechanistic history

Synthesis pass · year-by-year structured walk · 2 steps
  1. 2015 Medium

    Identification of KATNBL1 as a ciliary basal-body protein required for ciliogenesis and ciliary microtubule integrity resolved the cellular function of this previously uncharacterized gene and linked it to ciliopathy.

    Evidence Patient fibroblast phenotyping (null mutation), C. elegans epistasis with arl-13, in vitro microtubule binding, immunofluorescence localization

    PMID:26714646

    Open questions at the time
    • Mechanism by which KATNBL1 controls ciliary A-tubule number is undefined
    • Whether microtubule-stabilizing activity observed upon overexpression reflects physiological function is unclear
    • Genetic interaction with ARL13B shown only in C. elegans; conservation in mammals not tested
  2. 2016 Medium

    Systematic mapping of the katanin interaction network established KATNBL1 as a bona fide katanin B-like regulatory subunit that directly modulates KATNA1/KATNAL1 severing activity and competes with KATNB1 for catalytic subunit binding, revealing a competitive regulatory logic within the katanin family.

    Evidence AP-MS, reciprocal co-immunoprecipitation, in vitro microtubule-severing assay, competitive binding co-IP, fluorescence microscopy and subcellular fractionation

    PMID:26929214

    Open questions at the time
    • Structural basis for competition between KATNBL1 and KATNB1 for katanin A subunits is unknown
    • Whether KATNBL1 stimulates or inhibits severing activity (direction of regulation) was not fully resolved
    • Mitotic relocalization mechanism beyond NLS identification is not defined

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how nuclear sequestration of KATNBL1 during interphase is relieved at mitotic entry, how KATNBL1 and KATNB1 partitioning between katanin A subunits is regulated in vivo, and whether KATNBL1-dependent microtubule severing contributes to spindle assembly or is primarily required for ciliogenesis.
  • No in vivo reconstitution of KATNBL1-dependent severing in mammalian cells
  • No structural model for KATNBL1–KATNA1 interaction
  • Relative contributions of KATNBL1 to mitotic spindle vs. ciliary functions are unresolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 2 GO:0008092 cytoskeletal protein binding 1
Localization
GO:0005815 microtubule organizing center 2 GO:0005634 nucleus 1 GO:0005856 cytoskeleton 1
Pathway
R-HSA-1640170 Cell Cycle 1 R-HSA-1852241 Organelle biogenesis and maintenance 1
Partners
KATNA1KATNAL1KATNB1
Complex memberships
katanin complex

Evidence

Reading pass · 5 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2016 KATNBL1 associates with katanin A subunits KATNA1 and KATNAL1, as determined by mass spectrometry-based proteomics and co-immunoprecipitation, defining its position within the mammalian katanin family interaction network (Katan-ome). Affinity purification-mass spectrometry (AP-MS) and co-immunoprecipitation Molecular & cellular proteomics : MCP Medium 26929214
2016 KATNBL1 regulates katanin A subunit (KATNA1 and KATNAL1) microtubule-severing activity in vitro, establishing it as a functional regulator of the catalytic subunits. In vitro microtubule-severing assay Molecular & cellular proteomics : MCP Medium 26929214
2016 During interphase, KATNBL1 is sequestered in the nucleus via an N-terminal nuclear localization signal, but relocalizes to spindle poles exclusively during mitosis. Fluorescence microscopy, subcellular fractionation, nuclear localization signal identification Molecular & cellular proteomics : MCP Medium 26929214
2016 KATNB1 (the canonical p80 regulatory subunit) can compete with KATNBL1 for interaction with KATNA1 and KATNAL1, suggesting that KATNBL1 and KATNB1 coordinate to regulate katanin A subunit activity. Competitive co-immunoprecipitation Molecular & cellular proteomics : MCP Medium 26929214
2015 KIAA0556 (KATNBL1) localizes to the ciliary basal body, and patient-derived fibroblasts with a null mutation display reduced ciliogenesis and abnormally elongated cilia. In C. elegans, the ortholog regulates ciliary A-tubule number and genetically interacts with ARL13B. Human KATNBL1 binds microtubules in vitro, appears to stabilize microtubule networks upon overexpression, and biochemically interacts with p60/p80 katanins. Patient fibroblast phenotyping, C. elegans genetics (epistasis with arl-13), in vitro microtubule binding assay, immunofluorescence localization, biochemical interaction assay Genome biology Medium 26714646

Source papers

Stage 0 corpus · 31 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2000 DNA cloning using in vitro site-specific recombination. Genome research 815 11076863
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2000 Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO reports 281 11256614
2011 A directed protein interaction network for investigating intracellular signal transduction. Science signaling 258 21900206
2019 Network-based prediction of protein interactions. Nature communications 184 30886144
2001 Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. Genome research 151 11230166
2009 Ubiquitin-mediated proteolysis of HuR by heat shock. The EMBO journal 142 19322201
2017 Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex. Nature cell biology 129 28436967
2017 A Global Analysis of the Receptor Tyrosine Kinase-Protein Phosphatase Interactome. Molecular cell 102 28065597
2019 Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations. Nature communications 60 31515488
2015 KIAA0556 is a novel ciliary basal body component mutated in Joubert syndrome. Genome biology 60 26714646
2022 NUDT21 limits CD19 levels through alternative mRNA polyadenylation in B cell acute lymphoblastic leukemia. Nature immunology 46 36138187
2016 Proteomic Analysis of the Mammalian Katanin Family of Microtubule-severing Enzymes Defines Katanin p80 subunit B-like 1 (KATNBL1) as a Regulator of Mammalian Katanin Microtubule-severing. Molecular & cellular proteomics : MCP 42 26929214
2011 The spindle assembly function of Caenorhabditis elegans katanin does not require microtubule-severing activity. Molecular biology of the cell 39 21372175
2004 From ORFeome to biology: a functional genomics pipeline. Genome research 38 15489336
2021 Comparative Host Interactomes of the SARS-CoV-2 Nonstructural Protein 3 and Human Coronavirus Homologs. Molecular & cellular proteomics : MCP 29 34186245
2006 The LIFEdb database in 2006. Nucleic acids research 29 16381901
2020 SUMOylation of DDX39A Alters Binding and Export of Antiviral Transcripts to Control Innate Immunity. Journal of immunology (Baltimore, Md. : 1950) 27 32393512
2005 Transcriptome analysis of human gastric cancer. Mammalian genome : official journal of the International Mammalian Genome Society 24 16341674
2024 PLAG1-Rearranged Uterine Sarcomas: A Study of 11 Cases Showing a Wide Phenotypical Spectrum Not Limited to Myxoid Leiomyosarcoma-Like Morphology. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 12 38942115
2022 TRABID targets DDB2 for deubiquitination to promote proliferation of hepatocellular carcinoma cells. Biochemical and biophysical research communications 9 35944360