{"gene":"KATNBL1","run_date":"2026-06-10T01:55:23","timeline":{"discoveries":[{"year":2016,"finding":"KATNBL1 associates with katanin A subunits KATNA1 and KATNAL1, as determined by mass spectrometry-based proteomic interaction mapping (Katan-ome).","method":"Mass spectrometry proteomics / co-immunoprecipitation interaction network","journal":"Molecular & cellular proteomics : MCP","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — MS-based interactome plus Co-IP, single lab, two orthogonal methods","pmids":["26929214"],"is_preprint":false},{"year":2016,"finding":"KATNBL1 regulates Katanin A subunit (KATNA1 and KATNAL1) microtubule-severing activity, as demonstrated by an in vitro microtubule-severing assay.","method":"In vitro microtubule-severing assay","journal":"Molecular & cellular proteomics : MCP","confidence":"High","confidence_rationale":"Tier 1 / Moderate — direct in vitro enzymatic assay, single lab but clear functional readout with defined molecular partners","pmids":["26929214"],"is_preprint":false},{"year":2016,"finding":"During interphase, KATNBL1 is sequestered in the nucleus via an N-terminal nuclear localization signal; during mitosis, it relocates to spindle poles.","method":"Subcellular localization by fluorescence imaging; NLS identification by sequence analysis and functional localization experiments","journal":"Molecular & cellular proteomics : MCP","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct localization imaging tied to cell-cycle-stage-specific function, single lab","pmids":["26929214"],"is_preprint":false},{"year":2016,"finding":"KATNB1 competes with KATNBL1 for binding to KATNA1 and KATNAL1, suggesting coordinated regulation of katanin activity by both B subunits.","method":"Competition binding assay (co-immunoprecipitation)","journal":"Molecular & cellular proteomics : MCP","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — Co-IP competition assay, single lab, single method","pmids":["26929214"],"is_preprint":false}],"current_model":"KATNBL1 is a regulatory B-like subunit of the katanin microtubule-severing complex that is sequestered in the nucleus during interphase via an N-terminal NLS, relocates to spindle poles during mitosis, physically associates with the catalytic A subunits KATNA1 and KATNAL1, and directly regulates their microtubule-severing activity; KATNB1 can compete KATNBL1 off these A subunits, indicating coordinated control of katanin activity by both B-type subunits."},"narrative":{"mechanistic_narrative":"KATNBL1 is a regulatory B-like subunit of the katanin microtubule-severing machinery that controls the catalytic A subunits KATNA1 and KATNAL1 [PMID:26929214]. It physically associates with both A subunits and directly modulates their microtubule-severing activity in vitro [PMID:26929214]. KATNBL1 displays cell-cycle-dependent localization: an N-terminal nuclear localization signal sequesters it in the nucleus during interphase, and it relocates to spindle poles during mitosis, coupling its regulatory action to specific cell-cycle stages [PMID:26929214]. The canonical B subunit KATNB1 competes with KATNBL1 for binding to KATNA1 and KATNAL1, indicating that the two B-type subunits coordinately tune katanin activity [PMID:26929214]. Beyond these interaction, localization, and severing-regulation findings, no further mechanistic detail has been characterized in the available corpus.","teleology":[{"year":2016,"claim":"Establishing the physical partners of KATNBL1 was needed to place it within the katanin system; proteomic mapping showed it associates with the catalytic A subunits KATNA1 and KATNAL1.","evidence":"Mass spectrometry interactome and co-immunoprecipitation in the Katan-ome network","pmids":["26929214"],"confidence":"Medium","gaps":["Binding interface and stoichiometry with KATNA1/KATNAL1 not defined","No reciprocal validation across independent labs"]},{"year":2016,"claim":"Whether KATNBL1 functionally affects katanin catalysis, not merely binds it, was open; an in vitro severing assay showed it regulates KATNA1 and KATNAL1 microtubule-severing activity.","evidence":"Reconstituted in vitro microtubule-severing assay with defined A subunits","pmids":["26929214"],"confidence":"High","gaps":["Direction and magnitude of regulation (activation vs inhibition) not detailed here","Structural basis of the regulatory effect unknown"]},{"year":2016,"claim":"How KATNBL1 activity is spatially and temporally restricted was unknown; imaging revealed NLS-mediated nuclear sequestration in interphase and relocation to spindle poles in mitosis.","evidence":"Fluorescence subcellular localization with NLS identification and functional localization experiments","pmids":["26929214"],"confidence":"Medium","gaps":["Mechanism and regulation of mitotic relocation to spindle poles unknown","Functional consequence of nuclear sequestration not established"]},{"year":2016,"claim":"Whether the two B-type subunits act independently or competitively was unresolved; competition binding showed KATNB1 displaces KATNBL1 from KATNA1 and KATNAL1.","evidence":"Co-immunoprecipitation competition assay","pmids":["26929214"],"confidence":"Medium","gaps":["Quantitative affinities and physiological balance between KATNB1 and KATNBL1 not measured","Functional outcome of competition on severing activity in cells not shown"]},{"year":null,"claim":"The direction of KATNBL1's regulatory effect on severing, the structural basis of A-subunit binding, and the in vivo consequences of its nuclear-to-spindle-pole shuttling remain unresolved.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No structural model of the KATNBL1–A subunit interaction","In vivo phenotype of KATNBL1 loss not characterized in the corpus","Regulatory mechanism of cell-cycle-dependent relocalization unknown"]}],"mechanism_profile":{"molecular_activity":[{"term_id":"GO:0098772","term_label":"molecular function regulator activity","supporting_discovery_ids":[1,3]},{"term_id":"GO:0008092","term_label":"cytoskeletal protein binding","supporting_discovery_ids":[0,1]}],"localization":[{"term_id":"GO:0005634","term_label":"nucleus","supporting_discovery_ids":[2]},{"term_id":"GO:0005815","term_label":"microtubule organizing center","supporting_discovery_ids":[2]}],"pathway":[],"complexes":["katanin complex"],"partners":["KATNA1","KATNAL1","KATNB1"],"other_free_text":[]}},"prefetch_data":{"uniprot":{"accession":"Q9H079","full_name":"KATNB1-like protein 1","aliases":["Katanin p80 subunit B-like 1"],"length_aa":304,"mass_kda":34.8,"function":"Regulates microtubule-severing activity of KATNAL1 in a concentration-dependent manner in vitro","subcellular_location":"Nucleus; Cytoplasm, cytoskeleton, spindle pole","url":"https://www.uniprot.org/uniprotkb/Q9H079/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/KATNBL1","classification":"Not Classified","n_dependent_lines":2,"n_total_lines":1208,"dependency_fraction":0.0016556291390728477},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[],"url":"https://opencell.sf.czbiohub.org/search/KATNBL1","total_profiled":1310},"omim":[{"mim_id":"616650","title":"KATANIN-INTERACTING PROTEIN; KATNIP","url":"https://www.omim.org/entry/616650"},{"mim_id":"616235","title":"KATANIN, p80 SUBUNIT, B-LIKE 1; KATNBL1","url":"https://www.omim.org/entry/616235"}],"hpa":{"profiled":true,"resolved_as":"","reliability":"Supported","locations":[{"location":"Midbody","reliability":"Supported"},{"location":"Mitotic spindle","reliability":"Supported"},{"location":"Nucleoplasm","reliability":"Additional"},{"location":"Cleavage furrow","reliability":"Additional"}],"tissue_specificity":"Low tissue specificity","tissue_distribution":"Detected in all","driving_tissues":[],"url":"https://www.proteinatlas.org/search/KATNBL1"},"hgnc":{"alias_symbol":["FLJ22557"],"prev_symbol":["C15orf29"]},"alphafold":{"accession":"Q9H079","domains":[{"cath_id":"1.25.40","chopping":"155-301","consensus_level":"high","plddt":93.3828,"start":155,"end":301}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/Q9H079","model_url":"https://alphafold.ebi.ac.uk/files/AF-Q9H079-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-Q9H079-F1-predicted_aligned_error_v6.png","plddt_mean":70.12},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=KATNBL1","jax_strain_url":"https://www.jax.org/strain/search?query=KATNBL1"},"sequence":{"accession":"Q9H079","fasta_url":"https://rest.uniprot.org/uniprotkb/Q9H079.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/Q9H079/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/Q9H079"}},"corpus_meta":[{"pmid":"26929214","id":"PMC_26929214","title":"Proteomic Analysis of the Mammalian Katanin Family of Microtubule-severing Enzymes Defines Katanin p80 subunit B-like 1 (KATNBL1) as a Regulator of Mammalian Katanin Microtubule-severing.","date":"2016","source":"Molecular & cellular proteomics : MCP","url":"https://pubmed.ncbi.nlm.nih.gov/26929214","citation_count":42,"is_preprint":false},{"pmid":"38942115","id":"PMC_38942115","title":"PLAG1-Rearranged Uterine Sarcomas: A Study of 11 Cases Showing a Wide Phenotypical Spectrum Not Limited to Myxoid Leiomyosarcoma-Like Morphology.","date":"2024","source":"Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc","url":"https://pubmed.ncbi.nlm.nih.gov/38942115","citation_count":14,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3772,"output_tokens":845,"usd":0.011995,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":7256,"output_tokens":1843,"usd":0.041177,"stage2_stop_reason":"end_turn"},"total_usd":0.053172,"stage1_batch_id":"msgbatch_01DUPACZi7AAWwuZiUjseQF4","stage2_batch_id":"msgbatch_018yt7chE3f48PEFtDwtj6AE","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2016,\n      \"finding\": \"KATNBL1 associates with katanin A subunits KATNA1 and KATNAL1, as determined by mass spectrometry-based proteomic interaction mapping (Katan-ome).\",\n      \"method\": \"Mass spectrometry proteomics / co-immunoprecipitation interaction network\",\n      \"journal\": \"Molecular & cellular proteomics : MCP\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — MS-based interactome plus Co-IP, single lab, two orthogonal methods\",\n      \"pmids\": [\"26929214\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2016,\n      \"finding\": \"KATNBL1 regulates Katanin A subunit (KATNA1 and KATNAL1) microtubule-severing activity, as demonstrated by an in vitro microtubule-severing assay.\",\n      \"method\": \"In vitro microtubule-severing assay\",\n      \"journal\": \"Molecular & cellular proteomics : MCP\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1 / Moderate — direct in vitro enzymatic assay, single lab but clear functional readout with defined molecular partners\",\n      \"pmids\": [\"26929214\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2016,\n      \"finding\": \"During interphase, KATNBL1 is sequestered in the nucleus via an N-terminal nuclear localization signal; during mitosis, it relocates to spindle poles.\",\n      \"method\": \"Subcellular localization by fluorescence imaging; NLS identification by sequence analysis and functional localization experiments\",\n      \"journal\": \"Molecular & cellular proteomics : MCP\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct localization imaging tied to cell-cycle-stage-specific function, single lab\",\n      \"pmids\": [\"26929214\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2016,\n      \"finding\": \"KATNB1 competes with KATNBL1 for binding to KATNA1 and KATNAL1, suggesting coordinated regulation of katanin activity by both B subunits.\",\n      \"method\": \"Competition binding assay (co-immunoprecipitation)\",\n      \"journal\": \"Molecular & cellular proteomics : MCP\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — Co-IP competition assay, single lab, single method\",\n      \"pmids\": [\"26929214\"],\n      \"is_preprint\": false\n    }\n  ],\n  \"current_model\": \"KATNBL1 is a regulatory B-like subunit of the katanin microtubule-severing complex that is sequestered in the nucleus during interphase via an N-terminal NLS, relocates to spindle poles during mitosis, physically associates with the catalytic A subunits KATNA1 and KATNAL1, and directly regulates their microtubule-severing activity; KATNB1 can compete KATNBL1 off these A subunits, indicating coordinated control of katanin activity by both B-type subunits.\"\n}\n```","stage2_raw":"```json\n{\n  \"mechanistic_narrative\": \"KATNBL1 is a regulatory B-like subunit of the katanin microtubule-severing machinery that controls the catalytic A subunits KATNA1 and KATNAL1 [#0, #1]. It physically associates with both A subunits and directly modulates their microtubule-severing activity in vitro [#0, #1]. KATNBL1 displays cell-cycle-dependent localization: an N-terminal nuclear localization signal sequesters it in the nucleus during interphase, and it relocates to spindle poles during mitosis, coupling its regulatory action to specific cell-cycle stages [#2]. The canonical B subunit KATNB1 competes with KATNBL1 for binding to KATNA1 and KATNAL1, indicating that the two B-type subunits coordinately tune katanin activity [#3]. Beyond these interaction, localization, and severing-regulation findings, no further mechanistic detail has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2016,\n      \"claim\": \"Establishing the physical partners of KATNBL1 was needed to place it within the katanin system; proteomic mapping showed it associates with the catalytic A subunits KATNA1 and KATNAL1.\",\n      \"evidence\": \"Mass spectrometry interactome and co-immunoprecipitation in the Katan-ome network\",\n      \"pmids\": [\"26929214\"],\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate \\u2014 MS-based interactome plus Co-IP, single lab\",\n      \"gaps\": [\n        \"Binding interface and stoichiometry with KATNA1/KATNAL1 not defined\",\n        \"No reciprocal validation across independent labs\"\n      ]\n    },\n    {\n      \"year\": 2016,\n      \"claim\": \"Whether KATNBL1 functionally affects katanin catalysis, not merely binds it, was open; an in vitro severing assay showed it regulates KATNA1 and KATNAL1 microtubule-severing activity.\",\n      \"evidence\": \"Reconstituted in vitro microtubule-severing assay with defined A subunits\",\n      \"pmids\": [\"26929214\"],\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1 \\u2014 direct enzymatic readout with defined partners\",\n      \"gaps\": [\n        \"Direction and magnitude of regulation (activation vs inhibition) not detailed here\",\n        \"Structural basis of the regulatory effect unknown\"\n      ]\n    },\n    {\n      \"year\": 2016,\n      \"claim\": \"How KATNBL1 activity is spatially and temporally restricted was unknown; imaging revealed NLS-mediated nuclear sequestration in interphase and relocation to spindle poles in mitosis.\",\n      \"evidence\": \"Fluorescence subcellular localization with NLS identification and functional localization experiments\",\n      \"pmids\": [\"26929214\"],\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 \\u2014 direct imaging tied to cell-cycle stage, single lab\",\n      \"gaps\": [\n        \"Mechanism and regulation of mitotic relocation to spindle poles unknown\",\n        \"Functional consequence of nuclear sequestration not established\"\n      ]\n    },\n    {\n      \"year\": 2016,\n      \"claim\": \"Whether the two B-type subunits act independently or competitively was unresolved; competition binding showed KATNB1 displaces KATNBL1 from KATNA1 and KATNAL1.\",\n      \"evidence\": \"Co-immunoprecipitation competition assay\",\n      \"pmids\": [\"26929214\"],\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 \\u2014 Co-IP competition, single method\",\n      \"gaps\": [\n        \"Quantitative affinities and physiological balance between KATNB1 and KATNBL1 not measured\",\n        \"Functional outcome of competition on severing activity in cells not shown\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The direction of KATNBL1's regulatory effect on severing, the structural basis of A-subunit binding, and the in vivo consequences of its nuclear-to-spindle-pole shuttling remain unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"\",\n      \"gaps\": [\n        \"No structural model of the KATNBL1\\u2013A subunit interaction\",\n        \"In vivo phenotype of KATNBL1 loss not characterized in the corpus\",\n        \"Regulatory mechanism of cell-cycle-dependent relocalization unknown\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0098772\", \"supporting_discovery_ids\": [1, 3]},\n      {\"term_id\": \"GO:0008092\", \"supporting_discovery_ids\": [0, 1]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [2]},\n      {\"term_id\": \"GO:0005815\", \"supporting_discovery_ids\": [2]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [\"katanin complex\"],\n    \"partners\": [\"KATNA1\", \"KATNAL1\", \"KATNB1\"],\n    \"other_free_text\": []\n  }\n}\n```","audit_flag":null,"evaluation":{"pairwise":"tie","faith_supported":4,"faith_total":4,"faith_pct":100.0}}