Affinage

KANK1

KN motif and ankyrin repeat domain-containing protein 1 · UniProt Q14678

Length
1352 aa
Mass
147.3 kDa
Annotated
2026-04-28
57 papers in source corpus 22 papers cited in narrative 23 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

KANK1 is a mechanosensitive scaffold protein that links focal adhesions to cortical microtubule stabilizing complexes and modulates Rho-family GTPase signaling to coordinate cell polarity, migration, and adhesion dynamics. Its N-terminal KN domain binds directly to talin rod domain R7 with a force-resistant interaction that confines KANK1 to the focal adhesion periphery under actomyosin tension, while its C-terminal ankyrin repeat domain recruits KIF21A via a structurally defined dual-interface supramodule, thereby tethering microtubule plus-end machinery (CLASPs, LL5β, liprins) to adhesion sites (PMID:27410476, PMID:29158259, PMID:37874676, PMID:31389241). KANK1 suppresses RhoA activity — regulated by Akt-mediated phosphorylation and 14-3-3 binding — and inhibits Rac1-driven lamellipodia by blocking the IRSp53–Rac1 interaction, thereby restraining actin remodeling and cell spreading (PMID:18458160, PMID:19171758). KANK1 undergoes liquid–liquid phase separation at the adhesion belt and can undergo CRM1-dependent nucleo-cytoplasmic shuttling to activate β-catenin-dependent transcription; at cell–cell junctions it competes with Scribble for NOS1AP binding, suppressing Hippo pathway activation and promoting TAZ nuclear accumulation (PMID:37874676, PMID:16968744, PMID:39613731).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 2006 Medium

    Establishing that KANK1 is not solely cytoplasmic resolved how it could influence transcription: it possesses functional NLS and NES sequences, shuttles between nucleus and cytoplasm in a CRM1-dependent manner, binds β-catenin, and activates β-catenin-dependent transcription.

    Evidence NLS/NES mutagenesis, leptomycin B treatment, TOPFLASH reporter assay, Co-IP in cultured cells

    PMID:16968744

    Open questions at the time
    • Endogenous nuclear KANK1 levels not quantified under physiological conditions
    • β-catenin target genes regulated by KANK1 not identified
    • Relationship between nuclear shuttling and adhesion-site functions unclear
  2. 2008 High

    Identifying KANK1 as an Akt substrate explained how growth factor signaling tunes its cytoskeletal function: PI3K/Akt phosphorylation promotes 14-3-3 binding, which inactivates KANK1's RhoA-suppressive activity and permits stress fiber formation and cell migration.

    Evidence In vitro kinase assay, Co-IP, RhoA-GTP pulldown, migration assay in NIH3T3 cells

    PMID:18458160

    Open questions at the time
    • Phosphorylation site(s) not mapped to specific serines at that time
    • Whether 14-3-3 binding also affects KANK1 localization or other interactions unknown
  3. 2009 High

    Demonstrating that KANK1 inhibits Rac1 via blocking IRSp53–Rac1 interaction, selectively suppressing lamellipodia but not filopodia, established KANK1 as a dual negative regulator of both RhoA and Rac1 branches of Rho-family signaling.

    Evidence Co-IP, GST pulldown, RNAi knockdown, cell spreading and lamellipodia assays

    PMID:19171758

    Open questions at the time
    • Whether RhoA and Rac1 suppression are coordinated or independent remains unclear
    • Structural basis of KANK1–IRSp53 interaction not determined
  4. 2009 Medium

    Identifying KIF21A as a KANK1 interactor via the ankyrin repeat domain, and showing that KIF21A controls KANK1 subcellular distribution, provided the first link between KANK1 and microtubule motor function.

    Evidence Co-IP, subcellular fractionation, siRNA knockdown in cultured cells

    PMID:19559006

    Open questions at the time
    • Interaction mapped only by Co-IP and fractionation, no structural detail at that time
    • Functional consequence for microtubule dynamics not tested
  5. 2011 Medium

    The discovery that KANK1 associates with BIG1 and that both are required for MTOC/Golgi reorientation during wound healing established KANK1 as a polarity regulator acting through the ARF-GEF pathway.

    Evidence Reciprocal Co-IP, siRNA depletion, wound-healing migration assay, Golgi/MTOC orientation imaging

    PMID:22084092

    Open questions at the time
    • Whether KANK1–BIG1 interaction is direct or within a larger complex not resolved
    • ARF-dependent trafficking cargo regulated by this pathway not identified
  6. 2016 High

    The crystal structure of the KANK1 KN domain bound to talin R7, combined with a single point mutation that abolished recruitment without affecting adhesion, provided the definitive molecular mechanism by which KANK1 couples focal adhesions to cortical microtubule stabilizing complexes containing CLASPs, KIF21A, LL5β, and liprins.

    Evidence X-ray crystallography, Co-IP, single-point mutagenesis, fluorescence microscopy

    PMID:27410476

    Open questions at the time
    • How KANK1 discriminates between different focal adhesion subpopulations unclear
    • Stoichiometry of the KANK1–talin complex at adhesions not determined
  7. 2017 High

    High-resolution structures of the KANK1 ankyrin repeat–KIF21A complex revealed a dual-interface supramodule recognition mechanism and showed that cancer-associated mutations destabilize this interaction, defining the structural basis for microtubule effector recruitment.

    Evidence X-ray crystallography at 2.1 Å, site-directed mutagenesis, biochemical binding assays

    PMID:29158259 PMID:29183992 PMID:29217769

    Open questions at the time
    • Whether cancer-associated mutations affect KANK1 function in vivo not tested
    • How KIF21A recruitment alters microtubule dynamics at focal adhesions not directly measured
  8. 2017 Medium

    Demonstrating that KANK1 loss causes centrosome amplification and cytokinesis failure through Daam1-mediated RhoA hyperactivation expanded KANK1's role from adhesion scaffold to a regulator of cell division fidelity.

    Evidence RNAi knockdown, Co-IP of KANK1–Daam1, RhoA activity assay, centrosome counting, Aurora-A activity measurement

    PMID:28284839

    Open questions at the time
    • Whether KANK1–Daam1 interaction is direct not confirmed
    • Single cell line study; generalizability unclear
  9. 2019 High

    Single-molecule force spectroscopy revealed that the talin R7–KANK1 KN complex withstands physiological forces up to 10 pN, explaining why KANK1 is mechanically excluded from the high-tension FA center and confined to the peripheral adhesion belt.

    Evidence Magnetic tweezers single-molecule force spectroscopy, TIRF live-cell imaging

    PMID:31389241

    Open questions at the time
    • Force thresholds in cells not directly measured
    • Whether other adhesion proteins modulate force sensitivity of the complex unknown
  10. 2022 Medium

    Linking KANK1 depletion to YAP1 nuclear localization and impaired myogenic differentiation revealed that KANK1's actin-regulatory function feeds into the Hippo/YAP mechanotransduction axis.

    Evidence siRNA knockdown in C2C12 myoblasts, YAP1 phosphorylation and localization assays, myogenic differentiation markers

    PMID:35805114

    Open questions at the time
    • Whether YAP activation is direct or secondary to F-actin changes not resolved
    • Single cell type tested
  11. 2023 High

    Structures of KANK1 with both talin R7 and liprin-β, combined with LLPS demonstration, established that KANK1 undergoes phase separation at the adhesion belt to organize a scaffold bridging FA cores to adjacent liprin assemblies and modulate FA shape.

    Evidence X-ray crystallography, LLPS assays, live-cell imaging, immunofluorescence

    PMID:37339751 PMID:37874676

    Open questions at the time
    • LLPS regulation in vivo not characterized
    • Whether phase separation is required or merely correlative for function not fully tested
  12. 2023 Medium

    Showing that the KIF21A–KANK1–talin1 axis is required for dendritic spine morphogenesis and LTP extended KANK1's scaffolding function to neuronal synaptic plasticity.

    Evidence siRNA knockdown in neurons, rescue with binding-deficient mutants, LTP electrophysiology, behavioral assays

    PMID:38767486

    Open questions at the time
    • Neuronal cell type specificity not explored
    • Downstream signaling from KANK1 loss in neurons not identified
  13. 2024 High

    Demonstrating that KANK1 relocates to cell–cell junctions upon basement membrane detachment, where it competes with Scribble for NOS1AP binding to suppress Hippo pathway activation, revealed a context-dependent signaling role that promotes TAZ-driven tumor cell survival.

    Evidence In vivo PyMT mammary tumor model, KANK1 knockout, Co-IP competition assay, TAZ localization and Hippo reporters

    PMID:39613731

    Open questions at the time
    • Whether KANK1–NOS1AP competition occurs in non-tumor contexts unknown
    • Structural basis of KANK1–NOS1AP interaction not determined
  14. 2025 Medium

    Identifying KANK1 enrichment at the β-cell capillary interface and showing it directs insulin granule fusion toward capillaries via a talin–KANK1–liprin scaffold demonstrated a physiological secretory polarity role for KANK1.

    Evidence siRNA knockdown, Co-IP, TIRF imaging of granule fusion, insulin secretion assay in β-cells

    PMID:41380968

    Open questions at the time
    • In vivo validation in animal models not yet performed
    • Whether other KANK family members compensate in β-cells not tested

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: how KANK1 LLPS is regulated by post-translational modifications and mechanical signals, the full structural basis of the elongated KANK1 rod bridging focal adhesions to microtubules, and whether KANK1's nuclear, adhesion, and junctional functions are coordinated or independently regulated.
  • Full-length KANK1 structure not available
  • Coordination between nuclear shuttling, adhesion scaffolding, and junctional signaling roles unknown
  • Post-translational modification map beyond Akt phosphorylation incomplete

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 5 GO:0098772 molecular function regulator activity 4 GO:0008092 cytoskeletal protein binding 3
Localization
GO:0005856 cytoskeleton 5 GO:0005829 cytosol 2 GO:0005886 plasma membrane 2 GO:0005634 nucleus 1
Pathway
R-HSA-1500931 Cell-Cell communication 4 R-HSA-162582 Signal Transduction 4 R-HSA-1640170 Cell Cycle 1
Partners
ARFGEF1DAAM1IRSP53KIF21ANOS1APPPFIA1PPFIBP1TLN1
Complex memberships
Cortical microtubule stabilizing complex (CMSC)Talin-KANK1-liprin scaffold

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2016 KANK1 directly binds to talin rod domain R7 via its conserved KN domain, recruiting cortical microtubule stabilizing complexes (containing CLASPs, KIF21A, LL5β, and liprins) to focal adhesions. A single point mutation in talin disrupting KANK1 binding abolishes this recruitment without affecting talin's adhesion function. Structural studies (crystal structure), Co-IP, single point mutagenesis, fluorescence microscopy eLife High 27410476
2008 KANK1 is an Akt substrate downstream of PI3K; Akt-mediated phosphorylation of KANK1 promotes its binding to 14-3-3 proteins, which inhibits KANK1's suppression of RhoA activity, actin stress fiber formation, and insulin-induced cell migration. Kinase assay, co-immunoprecipitation, overexpression/co-expression in NIH3T3 cells, RhoA activity assay (GST-rhotekin pulldown), migration assay The Journal of cell biology High 18458160
2009 KANK1 binds IRSp53 and specifically inhibits the IRSp53–Rac1 interaction, suppressing lamellipodia formation without affecting filopodia (Cdc42-dependent), thereby negatively regulating actin remodeling and cell spreading. Co-immunoprecipitation, GST pulldown, RNAi knockdown, overexpression, microscopy The Journal of cell biology High 19171758
2017 Crystal structure of the KANK1 ankyrin repeat domain (ANKRD) in complex with a KIF21A peptide revealed that target recognition involves combinatorial use of two interfaces; mutations at either interface disrupt the KANK1–KIF21A interaction and prevent KIF21A recruitment to focal adhesions. X-ray crystallography (high-resolution crystal structure), mutagenesis, biochemical binding assays, immunofluorescence localization The Journal of biological chemistry High 29217769
2017 Crystal structure of the KANK1·KIF21A complex at 2.1 Å showed that a five-helix-bundle-capping domain immediately preceding the ANK repeats forms a supramodule with the ANK repeats to bind a conserved KIF21A peptide; cancer-associated missense mutations at this interface destabilize the complex. X-ray crystallography, biochemical assays, mutagenesis The Journal of biological chemistry High 29158259
2017 The KANK2 ankyrin domain binds the same ~22 amino acid KIF21A peptide as KANK1; both complex structures show KIF21A adopting helical conformations upon binding to two distinct pockets of the ankyrin domain. X-ray crystallography, site-directed mutagenesis, biochemical binding assays The Journal of biological chemistry High 29183992
2019 The talin R7–KANK1 KN domain complex can withstand physiological forces (up to 10 pN) for seconds to minutes under shear-force geometry; mechanical stretching promotes KANK1 localization to the periphery of focal adhesions rather than the center. Magnetic tweezers single-molecule force spectroscopy, cell biology (live cell imaging, TIRF) Nano letters High 31389241
2009 KANK1 interacts with the third and fourth coiled-coil domains of KIF21A via its ankyrin-repeat domain; KIF21A controls the subcellular distribution of KANK1, with KIF21A knockdown causing KANK1 to remain cytosolic, and the CFEOM1-associated KIF21A R954W mutation significantly enhancing KANK1 translocation to the membrane fraction. Co-immunoprecipitation, subcellular fractionation, siRNA knockdown, Western blotting Biochemical and biophysical research communications Medium 19559006
2011 KANK1 physically and functionally associates with BIG1 (a guanine nucleotide-exchange factor for ARFs); depletion of either BIG1 or KANK1 similarly disrupts directed cell migration and Golgi/MTOC orientation toward the wound edge, indicating both function in maintaining cell polarity during migration. Reciprocal co-immunoprecipitation, siRNA depletion, wound-healing migration assay, Golgi/MTOC orientation imaging PNAS Medium 22084092
2006 KANK1 contains functional nuclear localization signals (NLS1, NLS2) and nuclear export signals (NES1–NES3); nuclear export is CRM1-dependent (blocked by leptomycin B); KANK1 binds β-catenin and its nuclear import correlates with activation of β-catenin-dependent transcription (TOPFLASH reporter). NLS/NES mutagenesis, leptomycin B treatment, TOPFLASH reporter assay, co-immunoprecipitation, immunofluorescence Journal of cell science Medium 16968744
2017 KANK1 knockdown causes centrosomal amplification and cytokinesis failure via hyperactivation of RhoA; KANK1 interacts with Daam1 (a RhoA activator), and excess Daam1 upon KANK1 loss hyperactivates RhoA, elevating Aurora-A activity and leading to abnormal centrosome numbers and multinucleate cells. RNAi knockdown, co-immunoprecipitation (KANK1–Daam1), RhoA activity assay, centrosome counting, Aurora-A activity measurement Experimental cell research Medium 28284839
2015 In rat glomeruli and cultured human podocytes, KANK2 interacts with ARHGDIA (a RHO GTPase regulator); KANK2 knockdown increases active GTP-bound RHOA and decreases podocyte migration; RNAi of the Drosophila KANK homolog disrupts slit diaphragm and lacuna channel structures in nephrocytes. Co-immunoprecipitation, RhoA activity assay (GTP-pulldown), RNAi in Drosophila nephrocytes, zebrafish kank2 morpholino knockdown, immunofluorescence co-localization The Journal of clinical investigation Medium 25961457
2011 The KANK1-PDGFRβ fusion protein (from t(5;9) translocation) constitutively activates STAT5 and ERK1/2 in a JAK2-independent manner; three N-terminal coiled-coil domains of KANK1 are required for KANK1-PDGFRβ-induced oligomerization (homotrimers and heavier oligomers), signaling, and hematopoietic cell growth. Retroviral transduction of Ba/F3 cells and CD34+ progenitors, JAK inhibitor treatment, phosphorylation assays, mutagenesis of coiled-coil domains, gel filtration oligomerization analysis Haematologica Medium 21685469
2023 KANK1 structures in complex with talin R7 and liprin-β were determined; KANK1 undergoes liquid-liquid phase separation (LLPS) important for its localization at the focal adhesion edge; KANK1 scaffolds the FA core and associated proteins to modulate FA shape in response to mechanical force. X-ray crystallography, biochemical assays, LLPS assay, cell biological analysis (immunofluorescence, live-cell imaging) Cell reports High 37874676
2023 The talin-KANK1 complex structure revealed a novel β-hairpin motif in the KN region of KANK1 that stabilizes its α-helical talin-binding interface with high affinity; actomyosin forces on talin exclude KANK1 from the FA center while retaining it at the adhesion periphery (adhesion belt). Non-covalent crystallographic chaperone approach (crystal structure), site-directed mutagenesis, myosin inhibitor treatment, constitutively active vinculin expression, immunofluorescence Open biology High 37339751
2014 Drosophila Kank (ortholog of human KANK1) directly binds EB1, and this interaction is essential for Kank localization to microtubule plus ends in cultured cells; in late embryos Kank accumulates at muscle-tendon attachment sites. Direct binding assay, immunofluorescence, deletion mutant analysis PloS one Medium 25203404
2021 TRAIP promotes polyubiquitination and proteasomal degradation of KANK1, leading to downregulation of IGFBP3 and activation of the AKT pathway, thereby enhancing osteosarcoma cell invasion and proliferation. Co-immunoprecipitation, ubiquitination assay, proteasome inhibitor treatment, KANK1 overexpression/knockdown, AKT pathway readouts Cell death & disease Medium 34349117
2022 In C2C12 myoblasts, KANK1 depletion increases F-actin accumulation and promotes nuclear localization of YAP1 by reducing YAP1 phosphorylation in the cytoplasm, activating YAP1 target genes and promoting proliferation while inhibiting myogenic differentiation. siRNA knockdown, F-actin staining (phalloidin), YAP1 phosphorylation/localization by immunofluorescence and western blot, myogenic marker expression, myotube formation assay Cells Medium 35805114
2017 KANK1 restoration in MPNST cells induces apoptosis and inhibits growth via upregulation of CXXC5; knockdown of CXXC5 diminishes KANK1-induced apoptosis, placing CXXC5 downstream of KANK1 in apoptosis signaling. Overexpression and knockdown in cell lines, xenograft assay, RNA-seq, CXXC5 siRNA epistasis Scientific reports Medium 28067315
2025 KANK1 is locally enriched at the β-cell capillary interface and forms a complex linking talin (focal adhesion) to liprin-β1 (which anchors liprin-α1); KANK1 knockdown disrupts liprin-α1 subcellular localization, reduces glucose-induced insulin secretion, and misdirects insulin granule fusion away from the capillary interface. siRNA knockdown, co-immunoprecipitation, immunofluorescence localization, live-cell TIRF imaging of granule fusion, insulin secretion assay The Journal of biological chemistry Medium 41380968
2023 The KIF21A–KANK1 interaction is critical for dendritic spine morphogenesis and synaptic plasticity in neurons; knockdown of KIF21A or KANK1 inhibits spine morphogenesis and dendritic branching, rescued only by wild-type proteins but not by binding-deficient mutants (disrupting KIF21A–KANK1 or KANK1–talin1 interfaces). siRNA knockdown in neurons, rescue with binding-deficient mutants, LTP recording, cognitive behavioral assay Neural regeneration research Medium 38767486
2024 KANK1 localizes to the basal side of BM-attached epithelial tumor cells; upon BM contact loss, KANK1 relocates to cell-cell junctions where it competes with Scribble for NOS1AP binding, thereby reducing Scribble's ability to activate the Hippo pathway, leading to TAZ nuclear accumulation and tumor cell survival. In vivo PyMT mammary tumor model, KANK1 knockout, co-immunoprecipitation (KANK1–NOS1AP, Scribble–NOS1AP competition), TAZ/YAP nuclear localization assay, Hippo pathway reporters Nature communications High 39613731
2025 The hub protein LC8 binds multiple weak motifs in the intrinsically disordered linker L2 of KANK1 cooperatively, converting this ~600 aa disordered region into an elongated rod-like assembly (~35–50 nm) long enough to bridge the membrane–microtubule gap at focal adhesions. In-cell assays, biochemical binding assays, AlphaFold multivalent assembly prediction, electron microscopy structural analysis bioRxivpreprint Medium

Source papers

Stage 0 corpus · 57 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2015 KANK deficiency leads to podocyte dysfunction and nephrotic syndrome. The Journal of clinical investigation 153 25961457
2016 Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions. eLife 152 27410476
2005 Deletion of the ANKRD15 gene at 9p24.3 causes parent-of-origin-dependent inheritance of familial cerebral palsy. Human molecular genetics 107 16301218
2008 Kank regulates RhoA-dependent formation of actin stress fibers and cell migration via 14-3-3 in PI3K-Akt signaling. The Journal of cell biology 99 18458160
2002 A novel ankyrin repeat-containing gene (Kank) located at 9p24 is a growth suppressor of renal cell carcinoma. The Journal of biological chemistry 87 12133830
2009 Kank proteins: structure, functions and diseases. Cellular and molecular life sciences : CMLS 85 19554261
2020 CircDDX17 reduces 5-fluorouracil resistance and hinders tumorigenesis in colorectal cancer by regulating miR-31-5p/KANK1 axis. European review for medical and pharmacological sciences 49 32141542
2009 Kank attenuates actin remodeling by preventing interaction between IRSp53 and Rac1. The Journal of cell biology 44 19171758
2007 Kank proteins: a new family of ankyrin-repeat domain-containing proteins. Biochimica et biophysica acta 43 17996375
2011 Effects of brefeldin A-inhibited guanine nucleotide-exchange (BIG) 1 and KANK1 proteins on cell polarity and directed migration during wound healing. Proceedings of the National Academy of Sciences of the United States of America 34 22084092
2021 TRAIP modulates the IGFBP3/AKT pathway to enhance the invasion and proliferation of osteosarcoma by promoting KANK1 degradation. Cell death & disease 31 34349117
2009 A major mutation of KIF21A associated with congenital fibrosis of the extraocular muscles type 1 (CFEOM1) enhances translocation of Kank1 to the membrane. Biochemical and biophysical research communications 30 19559006
2019 Force-Dependent Regulation of Talin-KANK1 Complex at Focal Adhesions. Nano letters 29 31389241
2017 KANK1 inhibits cell growth by inducing apoptosis through regulating CXXC5 in human malignant peripheral nerve sheath tumors. Scientific reports 29 28067315
2006 Nucleo-cytoplasmic shuttling of human Kank protein accompanies intracellular translocation of beta-catenin. Journal of cell science 29 16968744
2018 The Kank family proteins in adhesion dynamics. Current opinion in cell biology 28 29909279
2012 The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the α-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK. Angewandte Chemie (International ed. in English) 25 22374809
2017 Structural insights into ankyrin repeat-mediated recognition of the kinesin motor protein KIF21A by KANK1, a scaffold protein in focal adhesion. The Journal of biological chemistry 24 29217769
2020 KANK1 regulates paclitaxel resistance in lung adenocarcinoma A549 cells. Artificial cells, nanomedicine, and biotechnology 21 32064933
2011 Multiple oligomerization domains of KANK1-PDGFRβ are required for JAK2-independent hematopoietic cell proliferation and signaling via STAT5 and ERK. Haematologica 21 21685469
2016 Evolutionary and developmental analysis reveals KANK genes were co-opted for vertebrate vascular development. Scientific reports 20 27292017
2019 Aberrant Kank1 expression regulates YAP to promote apoptosis and inhibit proliferation in OSCC. Journal of cellular physiology 19 31338836
2017 In vivo and in vitro inhibition of human gastric cancer progress by upregulating Kank1 gene. Oncology reports 19 28731169
2013 Familial KANK1 deletion that does not follow expected imprinting pattern. European journal of medical genetics 19 23454270
2005 Pathological characterization of Kank in renal cell carcinoma. Experimental and molecular pathology 19 15596059
2020 KANK family proteins in cancer. The international journal of biochemistry & cell biology 18 33309958
2022 Kank1 Is Essential for Myogenic Differentiation by Regulating Actin Remodeling and Cell Proliferation in C2C12 Progenitor Cells. Cells 16 35805114
2014 Upregulation of the Kank1 gene-induced brain glioma apoptosis and blockade of the cell cycle in G0/G1 phase. International journal of oncology 16 24399197
2018 Clinical significance of copy number variants involving KANK1 in patients with neurodevelopmental disorders. European journal of medical genetics 15 29729439
2017 Structural analyses of key features in the KANK1·KIF21A complex yield mechanistic insights into the cross-talk between microtubules and the cell cortex. The Journal of biological chemistry 15 29158259
2017 Structural basis for the recognition of kinesin family member 21A (KIF21A) by the ankyrin domains of KANK1 and KANK2 proteins. The Journal of biological chemistry 15 29183992
2020 Long non-coding RNA CASC2 enhances cisplatin sensitivity in oral squamous cell cancer cells by the miR-31-5p/KANK1 axis. Neoplasma 14 32787433
2018 Upregulation of the Kank1 gene inhibits human lung cancer progression in vitro and in vivo. Oncology reports 14 29956815
2023 KANK1 shapes focal adhesions by orchestrating protein binding, mechanical force sensing, and phase separation. Cell reports 13 37874676
2023 The structural basis of the talin-KANK1 interaction that coordinates the actin and microtubule cytoskeletons at focal adhesions. Open biology 11 37339751
2021 LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis. Frontiers in microbiology 11 34917045
2015 Interstitial 9p24.3 deletion involving only DOCK8 and KANK1 genes in two patients with non-overlapping phenotypic traits. European journal of medical genetics 11 26656975
2005 Alternative splicing of the human Kank gene produces two types of Kank protein. Biochemical and biophysical research communications 11 15823577
2014 Kank Is an EB1 interacting protein that localises to muscle-tendon attachment sites in Drosophila. PloS one 10 25203404
2017 Depletion of tumor suppressor Kank1 induces centrosomal amplification via hyperactivation of RhoA. Experimental cell research 9 28284839
2016 Enrichment of small pathogenic deletions at chromosome 9p24.3 and 9q34.3 involving DOCK8, KANK1, EHMT1 genes identified by using high-resolution oligonucleotide-single nucleotide polymorphism array analysis. Molecular cytogenetics 9 27891178
2015 Kank1 reexpression induced by 5-Aza-2'-deoxycytidine suppresses nasopharyngeal carcinoma cell proliferation and promotes apoptosis. International journal of clinical and experimental pathology 8 25973051
2024 A germline chimeric KANK1-DMRT1 transcript derived from a complex structural variant is associated with a congenital heart defect segregating across five generations. Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 7 38504027
2023 The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons. Neural regeneration research 7 38767486
2020 Tissue distribution and subcellular localization of the family of Kidney Ankyrin Repeat Domain (KANK) proteins. Experimental cell research 7 33253712
2020 KANK1-NTRK3 fusions define a subset of BRAF mutation negative renal metanephric adenomas. BMC medical genetics 6 33046021
2024 Potential involvement of KANK1 haploinsufficiency in centrosome aberrations. Biochimica et biophysica acta. General subjects 3 38830559
2024 KANK1 promotes breast cancer development by compromising Scribble-mediated Hippo activation. Nature communications 3 39613731
2024 cNPAS2 induced β cell dysfunction by regulating KANK1 expression in type 2 diabetes. World journal of diabetes 2 39280178
2023 Loss of the KN Motif and AnKyrin Repeat Domain 1 (KANK1) Leads to Lymphoid Compartment Dysregulation in Murine Model. Genes 2 37895296
2021 Case Report: A Pancreatic Ductal Adenocarcinoma Patient With Concurrent Targetable Somatic Novel KANK1-ALK, UPP2-NTRK3 Fusion, and Pathogenetic Germline BRCA Mutation. Frontiers in oncology 2 34671564
2019 Small interstitial 9p24.3 deletions principally involving KANK1 are likely benign copy number variants. European journal of medical genetics 2 30684669
2024 Response of a Novel KANK1::ALK Fusion to Alectinib in an Advanced Lung Adenocarcinoma: A Case Report. Journal of the National Comprehensive Cancer Network : JNCCN 1 38364363
2025 Identification of KANK1 as a tumor suppressor gene in pancreatic ductal adenocarcinoma. Biochemical and biophysical research communications 0 40288262
2025 KANK1 regulates the positioning of liprin-α1 and the spatial organization of insulin granule fusion in pancreatic β cells. The Journal of biological chemistry 0 41380968
2023 A germline chimeric KANK1-DMRT1 transcript derived from a complex structural variant is associated with a congenital heart defect segregating across five generations. Research square 0 38168413
2022 Long non-coding RNA H19X promotes tumorigenesis and metastasis of colorectal cancer through regulating the miR-503-5p/KANK1 axis. Genes & genomics 0 35567714