Affinage

INO80

Chromatin-remodeling ATPase INO80 · UniProt Q9ULG1

Length
1556 aa
Mass
176.8 kDa
Annotated
2026-04-28
100 papers in source corpus 56 papers cited in narrative 56 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

INO80 is a conserved, multisubunit ATP-dependent chromatin remodeling complex that integrates nucleosome sliding, histone variant (H2A.Z) exchange, and hexasome remodeling to regulate transcription, DNA repair, replication fork stability, and genome integrity. The SNF2-family ATPase motor engages nucleosomal DNA at superhelical location −6 while the Arp5–Ies6 counter-grip module contacts SHL −2/−3, and the Arp8–Arp4–actin regulatory module senses extranucleosomal linker DNA length to allosterically gate remodeling activity, producing a DNA-length-sensitive switch with ~100-fold rate increase as flanking DNA extends from 40 to 60 bp (PMID:29452642, PMID:29643509, PMID:30120252, PMID:36490333). INO80 is recruited to DNA double-strand breaks via γH2AX interaction and promotes end resection, H2A.Z removal, presynaptic filament formation, and DSB relocation to the nuclear periphery for homologous recombination; it similarly facilitates nucleotide excision repair by restoring nucleosome structure after lesion removal and resolves R-loops to prevent replication-associated damage (PMID:15607975, PMID:26142279, PMID:28514650, PMID:21135142, PMID:32913330). At replication forks, INO80 stabilizes the replisome, promotes fork progression and restart after stalling—recruited via ubiquitinated H2A and stabilized by BAP1—while at gene promoters it positions the +1 nucleosome through DNA-shape readout, maintains open chromatin at pluripotency and superenhancer loci via Mediator recruitment, and context-dependently either removes or promotes H2A.Z occupancy to regulate transcriptional programs (PMID:18376411, PMID:25283999, PMID:24792115, PMID:27340176, PMID:34139016, PMID:34050142).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 2004 High

    Establishing that INO80 is a DNA damage-responsive complex: it was unknown how chromatin remodelers participated in DSB repair until INO80 was shown to be recruited to breaks via γH2AX and shown to be required for end resection, placing chromatin remodeling upstream of DSB processing.

    Evidence ChIP at HO-induced DSBs in yeast with H2A-S129 and Nhp10 mutants, DNA damage sensitivity assays

    PMID:15607974 PMID:15607975

    Open questions at the time
    • Mechanism of INO80-mediated resection was undefined
    • Whether mammalian INO80 uses the same recruitment mechanism was unknown
    • Direct biochemical connection between remodeling activity and resection not established
  2. 2005 High

    Demonstrating conservation of core biochemical activities: human INO80 was purified and shown to possess nucleosome-stimulated ATPase and ATP-dependent nucleosome sliding activity, establishing that the remodeling mechanism is conserved from yeast to mammals.

    Evidence Biochemical purification, mass spectrometry, in vitro ATPase and sliding assays with human INO80

    PMID:16230350

    Open questions at the time
    • Histone exchange activity not yet detected
    • Subunit contributions to catalysis unresolved
    • Structural basis of sliding unknown
  3. 2006 High

    Revealing antagonism between INO80 and SWR1 at DSBs: the functional relationship between INO80 and H2A.Z was unclear until genetic epistasis showed that INO80 opposes SWR1-dependent H2A.Z incorporation at damage sites and is required for checkpoint adaptation.

    Evidence Genetic epistasis with ino80/swr1 double mutants, ChIP for H2A.Z at DSBs, checkpoint adaptation assays

    PMID:16951256

    Open questions at the time
    • Whether INO80 directly removes H2A.Z biochemically was unknown
    • Molecular basis of INO80–SWR1 antagonism unresolved
  4. 2007 High

    Expanding INO80 functions beyond DSB repair: INO80 was linked to transcription via YY1-dependent recruitment to target genes, to homologous recombination in mammalian cells, to checkpoint signaling via Ies4 phosphorylation by Mec1/Tel1, to telomere maintenance, and to sister chromatid cohesion, revealing the complex as a multifunctional genome maintenance machine.

    Evidence Co-IP of YY1–INO80, ChIP at YY1 targets and telomeres, HR reporter assays, Ies4 phospho-site mutagenesis, cohesion assays

    PMID:17471029 PMID:17562861 PMID:17693258 PMID:17721549 PMID:17762868 PMID:18026119

    Open questions at the time
    • YY1 structural role within INO80 architecture not resolved
    • How Ies4 phosphorylation mechanistically affects checkpoint undefined
    • Telomere function single-lab finding
  5. 2008 High

    Establishing INO80 as a replication fork factor: it was unknown whether chromatin remodelers directly support DNA replication until INO80 was shown to stabilize replisomes at stalled forks and to be essential for replication restart, with genome-wide enrichment at origins and stalled forks.

    Evidence Inducible Ino80 degradation, ChIP at origins, DNA fiber analysis, genome-wide ChIP, HU restart assays

    PMID:18376411 PMID:18406137

    Open questions at the time
    • Mechanism of replisome stabilization unknown
    • Whether INO80 remodels chromatin ahead of or behind forks not determined
  6. 2010 High

    Discovering INO80's histone exchange and spacing activities: INO80 was shown to catalyze ATP-dependent replacement of H2A.Z/H2B with H2A/H2B dimers and to center nucleosomes on DNA with precise spacing rules, establishing its dual biochemical outputs and revealing it requires extranucleosomal DNA but not the H4 tail.

    Evidence In vitro histone exchange with purified INO80, genome-wide H2A.Z ChIP in ino80 mutants, nucleosome sliding/spacing with tail deletions

    PMID:21135121 PMID:21241891

    Open questions at the time
    • How the same complex performs both sliding and exchange was unclear
    • Structural basis of H2A.Z selectivity unknown
  7. 2011 High

    Defining modular architecture and mammalian DNA repair function: the human INO80 complex was shown to comprise three functionally distinct modules assembled on different Ino80 ATPase domains, and mammalian INO80 was directly implicated in DSB end resection.

    Evidence Purification of subassemblies with in vitro ATPase assays; siRNA, comet, HR reporter, and ssDNA assays in mammalian cells

    PMID:21303910 PMID:21947284

    Open questions at the time
    • How modules communicate allosterically during remodeling was not resolved
    • Structural architecture of nucleosome-bound complex unknown
  8. 2013 High

    Characterizing the Arp8 histone-binding and actin regulatory mechanisms: crystal structure of Arp8 revealed how it contacts H3–H4 via an actin-fold insertion, and nuclear actin was shown to function as a monomer within INO80 with subdomain 2 contributing to chromatin interaction.

    Evidence Crystal structure of Arp8CTD, actin mutagenesis with in vitro remodeling assays

    PMID:23213201 PMID:23524535 PMID:24297934

    Open questions at the time
    • How Arp8 senses linker DNA was unknown
    • Role of nuclear actin in allosteric communication not resolved
  9. 2014 High

    Connecting INO80 to replication fork recruitment via ubiquitinated H2A/BAP1 and to pluripotency gene regulation: INO80 was shown to be recruited to forks through ubiquitinated H2A with BAP1-dependent stabilization, and to co-occupy pluripotency promoters with OCT4/WDR5 maintaining open chromatin for Mediator/Pol II recruitment.

    Evidence iPOND-ChIP, DNA fiber assays, mouse KO, ChIP-seq at pluripotency loci with RNAi dependency mapping

    PMID:24792115 PMID:25016522 PMID:25283999

    Open questions at the time
    • Whether H2A ubiquitination directly regulates INO80 remodeling activity was untested
    • Structural basis of OCT4/WDR5-mediated recruitment unknown
  10. 2015 High

    Demonstrating that H2A.Z removal is INO80's primary HR-promoting activity and discovering new functions in heterochromatin boundary maintenance and RNAPII turnover: rescue of INO80-depleted HR defects by H2A.Z co-depletion proved that H2A.Z eviction is the critical step; separately, INO80 was shown to block Dot1-mediated H3K79 methylation at heterochromatin borders and to form a ternary complex with Cdc48/VCP for degradation of ubiquitinated Rpb1.

    Evidence H2A.Z co-depletion rescue of HR, in vitro Dot1 blocking assay, Ino80-Cdc48-RNAPII co-IP and degradation assays

    PMID:25691465 PMID:26142279 PMID:26306040 PMID:26656161

    Open questions at the time
    • How INO80 physically blocks Dot1 access was structurally unresolved
    • Whether RNAPII degradation is coupled to remodeling activity unclear
  11. 2017 High

    Revealing the translocation mechanism and structural architecture: INO80 was shown to translocate DNA at the H2A–H2B interface (not H3–H4 as other remodelers), explaining its unique ability to exchange H2A.Z–H2B without chaperones; cryo-EM revealed assembly around a RUVBL1/2 heterohexamer stator, and dimerization via Ino80CTD was shown to enable cooperative nucleosome spacing.

    Evidence Site-directed crosslinking, chaperone-free exchange assays, cryo-EM at 9.6/4.1 Å, ATPase-dead dimer reconstitution

    PMID:28514650 PMID:28585918 PMID:28591576 PMID:28604691 PMID:29323271

    Open questions at the time
    • Near-atomic resolution of nucleosome-engaged complex not yet achieved
    • How dimerization is regulated in vivo was unknown
  12. 2018 High

    Achieving near-atomic structural understanding of the INO80–nucleosome complex and its DNA-length sensing mechanism: cryo-EM structures at 3.7–4.3 Å revealed the motor at SHL −6 with Arp5–Ies6 counter-grip at SHL −2/−3; the Arp8 module was crystallized showing it senses linker DNA 37–51 bp from the nucleosome edge, allosterically coupling motor engagement to linker length with ~100-fold rate modulation.

    Evidence Cryo-EM of Chaetomium and human INO80–nucleosome complexes, crystal structure of Arp8 module, single-molecule FRET, protein–DNA crosslinking

    PMID:29452642 PMID:29643506 PMID:29643509 PMID:30120252 PMID:30177756

    Open questions at the time
    • Structural basis for H2A.Z selectivity during exchange not resolved
    • How the Nhp10 auto-inhibitory mechanism couples to the structural rearrangement was not visualized
  13. 2020 High

    Discovering INO80's role in R-loop resolution: INO80 depletion increased R-loops and caused replication-associated DNA damage; RNase H1 overexpression rescued the replication defect, and artificial INO80 tethering resolved R-loops in cis, establishing a direct mechanistic link between chromatin remodeling and R-loop turnover.

    Evidence S9.6 immunofluorescence, DNA fiber assays, RNase H1 rescue, LacO tethering in cancer cells

    PMID:32913330

    Open questions at the time
    • Whether INO80 displaces RNA or remodels nucleosomes to expose R-loops for resolution is unknown
    • Generality beyond cancer cell lines not established
  14. 2021 High

    Revealing context-dependent directionality of H2A.Z regulation and DNA-shape readout: in primed pluripotent stem cells INO80 unexpectedly promotes H2A.Z deposition and H3K27me3 at bivalent promoters; genome-wide reconstitution showed INO80 reads DNA shape/mechanics through allosteric interplay between core and Arp8 modules for +1 nucleosome positioning.

    Evidence Conditional Ino80 KO with ChIP-seq for H2A.Z/H3K27me3, whole-genome chromatin reconstitution assays

    PMID:34050142 PMID:34139016

    Open questions at the time
    • Mechanism by which INO80 switches between H2A.Z removal and deposition modes is unknown
    • Whether DNA-shape readout contributes to damage-site targeting is untested
  15. 2022 High

    Structural elucidation of linker DNA sensing and hexasome preference: cryo-EM revealed the HSA/post-HSA lever connecting the Arp8 module to the motor for chemomechanical coupling; INO80 was shown to prefer hexasomes over nucleosomes by ~60-fold at short linkers, suggesting it remodels through transient H2A–H2B dislodgement.

    Evidence Cryo-EM of multiple INO80 conformational states, in vitro hexasome vs. nucleosome sliding assays, MNase-seq in ino80 mutants

    PMID:35597239 PMID:36490333

    Open questions at the time
    • Whether hexasome intermediates form during sliding in vivo is not demonstrated
    • Structural basis for YY1 recruitment clarified but functional role in remodeling regulation unclear
  16. 2023 High

    Resolving hexasome recognition and post-translational regulation of Ino80 stability: cryo-EM of INO80–hexasome showed that loss of one H2A–H2B triggers a spin-rotated catalytic core activated by exposed H3–H4; TORC1-Rpd3L-mediated deacetylation of Ino80-K929 protects it from autophagic degradation, linking metabolic signaling to chromatin remodeling output.

    Evidence Cryo-EM of INO80–hexasome, ATPase/sliding assays; MS-identified acetylation site mutagenesis, rapamycin treatment, autophagy assays

    PMID:36888706 PMID:37384673

    Open questions at the time
    • Whether the spin-rotation mechanism applies to full nucleosome sliding is unknown
    • How TORC1 regulation of Ino80 stability interfaces with other degradation pathways (e.g. TRIM3) is not integrated

Open questions

Synthesis pass · forward-looking unresolved questions
  • The molecular basis by which INO80 switches between H2A.Z removal and H2A.Z deposition in different genomic and cellular contexts remains unresolved, as does the structural mechanism of R-loop resolution and the in vivo relevance of hexasome intermediates during processive sliding.
  • No structural or reconstitution data for context-dependent H2A.Z deposition vs. removal switch
  • Mechanism of R-loop resolution (direct RNA displacement vs. nucleosome remodeling) undefined
  • In vivo evidence for hexasome intermediates during INO80-mediated sliding lacking

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140657 ATP-dependent activity 9 GO:0140096 catalytic activity, acting on a protein 4 GO:0003677 DNA binding 3
Localization
GO:0005694 chromosome 8 GO:0005634 nucleus 5
Pathway
R-HSA-73894 DNA Repair 9 R-HSA-4839726 Chromatin organization 7 R-HSA-69306 DNA Replication 5 R-HSA-74160 Gene expression (Transcription) 4 R-HSA-1640170 Cell Cycle 3
Partners
ACTINARP5ARP8BAP1IES6RUVBL1RUVBL2YY1
Complex memberships
Arp5-Ies6 subcomplexArp8-Arp4-actin moduleINO80 chromatin remodeling complex

Evidence

Reading pass · 56 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2004 INO80 complex is recruited to DNA double-strand breaks (DSBs) through a specific interaction with phosphorylated histone H2A (γ-H2AX/γH2AX); recruitment requires the Nhp10 HMG-like subunit of INO80 and the H2A phosphoacceptor S129, and loss of INO80 impairs conversion of DSBs into ssDNA (end resection). Chromatin immunoprecipitation (ChIP) at HO endonuclease-induced DSBs in yeast; genetic analysis of H2A phosphoacceptor and Nhp10 mutants Cell High 15607974 15607975
2004 INO80 complex (containing actin-related proteins Arp5 and Arp8) is required for DNA repair at DSBs; strains lacking INO80, ARP5, or ARP8 are hypersensitive to DNA damaging agents and deficient in processing DSB ends into ssDNA. Genetic loss-of-function (deletion mutants), sensitivity assays, ChIP Cell High 15607974 15607975
2005 The human INO80 (hINO80) complex contains orthologs of 8 of 15 yeast INO80 subunits plus at least five metazoan-specific subunits; it exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding. Biochemical purification, mass spectrometry, in vitro ATPase and nucleosome sliding assays The Journal of biological chemistry High 16230350
2006 Ino80 is required for cell cycle checkpoint adaptation following a persistent DSB; loss of Ino80 results in inability to maintain high levels of H2AX phosphorylation and increased incorporation of the Htz1 (H2A.Z) histone variant at the DSB by Swr1; Ino80 and Swr1 function antagonistically at DSB-flanking chromatin. Genetic epistasis (ino80 swr1 double mutants), ChIP, checkpoint adaptation assays in yeast Genes & development High 16951256
2007 SWR1 is recruited to DSBs in a γH2AX-dependent manner; INO80 (but not SWR1) is responsible for removal of H2A.Z, γH2AX, and core histones near the break; INO80-specific subunits Arp8 and Nhp10 are required for Mre11 nuclease and Mec1 kinase binding at DSBs and for end-resection and checkpoint activation. ChIP at HO-induced DSBs, genetic analysis of arp8 and nhp10 mutants vs. swr1 mutants The EMBO journal High 17762868
2007 YY1 is tightly associated with the human INO80 chromatin-remodeling complex; YY1 recruits INO80 to YY1-activated target genes where INO80 functions as an essential transcriptional coactivator; binding of YY1 to its DNA target sites requires INO80 complex activity. Co-immunoprecipitation, ChIP, RNAi knockdown with transcriptional readout Nature structural & molecular biology High 17721549 18026119
2007 YY1 forms a complex with components of the INO80 chromatin-remodeling complex; both YY1 and INO80 are required for homologous recombination-based DNA repair (HRR) in mammalian cells; YY1 preferentially binds a recombination-intermediate DNA structure in vitro. Biochemical co-purification, RNAi knockdown, HR functional assays, in vitro DNA binding Nature structural & molecular biology High 18026119
2007 Mec1/Tel1 (ATM/ATR) kinases phosphorylate the Ies4 subunit of the INO80 complex during DNA damage; mutation of Ies4 phosphorylation sites does not affect DNA repair but influences DNA damage checkpoint responses and links INO80 to checkpoint regulators Tof1 and Rad53. In vivo phosphorylation assays, site-directed mutagenesis of Ies4 phospho-sites, checkpoint assays Cell High 17693258
2007 The Ies3 subunit of yeast INO80 interacts with the tetratricopeptide repeat domain of telomerase subunit Est1p; INO80 subunits localize to telomeres and regulate telomere length, telomere position effect, and recombinational telomere maintenance. Co-immunoprecipitation, ChIP at telomeres, genetic analysis of IES3 and ARP8 deletions Molecular and cellular biology Medium 17562861
2008 The Ino80 remodeling enzyme is required for progression of DNA replication forks and for stabilizing the replisome at stalled forks; stalling of replication forks in ino80 mutants is lethal and causes dissociation of replication machinery from stalled forks. Inducible degradation of Ino80, ChIP at replication origins, DNA fiber analysis, 2D gel electrophoresis Nature structural & molecular biology High 18376411
2008 INO80 complex binds at replication origins and tRNA genes genome-wide; INO80 enrichment at stalled replication forks increases upon hydroxyurea treatment; ino80 mutants fail to resume DNA replication after HU block and accumulate DSBs during replication restart. Genome-wide ChIP, hydroxyurea treatment, replication restart assays in yeast Current biology : CB High 18406137
2008 Uch37 deubiquitinating enzyme is associated with the human INO80 chromatin-remodeling complex in the nucleus, where it is held in an inactive state; Uch37 DUB activity can be activated by transient interaction of the INO80 complex with the proteasome. Co-immunoprecipitation, DUB activity assays in the presence/absence of INO80 or proteasome Molecular cell High 18922472
2009 INO80 acts in the same genetic pathway as nucleotide excision repair (NER); Ino80 interacts with the early NER damage recognition complex Rad4-Rad23; Ino80 is recruited to chromatin by Rad4 in a UV damage-dependent manner and is required for restoration of nucleosome structure after NER, not for chromatin disruption during repair. Epistasis analysis, Co-immunoprecipitation (Ino80-Rad4-Rad23), modified ChIP assay for nucleosome restoration The Journal of cell biology High 21135142
2010 INO80 has a histone-exchange activity: it can replace nucleosomal H2A.Z/H2B with free H2A/H2B dimers; loss of INO80 causes mislocalization of H2A.Z genome-wide, with reduced responsiveness of H2A.Z at promoters to transcriptional changes. In vitro histone exchange assay with purified INO80, genome-wide H2A.Z ChIP in ino80 mutants Cell High 21241891
2010 INO80 promotes nucleosome spacing: it moves nucleosomes to the center of DNA with high precision and requires a minimum of 33-43 bp extranucleosomal DNA; unlike ISWI remodelers, INO80 does not require the H4 tail but is negatively regulated by the H2A tail. In vitro nucleosome sliding and spacing assays with mononucleosomes and arrays, histone tail deletion mutants Molecular and cellular biology High 21135121
2010 The mammalian INO80 complex is recruited to laser-induced DNA damage sites in an ARP8-dependent but γH2AX-independent manner, in contrast to yeast INO80 which requires Nhp10 or Arp4 for recruitment. Live imaging of GFP-tagged subunits at laser-induced damage; ARP8 knockdown Biochemical and biophysical research communications Medium 20971067
2010 INO80 (Ino80 and Arp5 subunits) promotes removal of UV lesions by the nucleotide excision repair (NER) pathway; loss of INO80 abolishes assembly of NER factors at damage; Ino80 and Arp5 are enriched at UV-damaged DNA prior to NER incision, functioning in chromatin relaxation for NER access. Genetic deletion models, NER factor assembly ChIP, UV photoproduct removal assays Proceedings of the National Academy of Sciences of the United States of America High 20855601
2011 Mammalian Ino80 mediates DSB repair through DNA end strand resection; Ino80 depletion impairs focal recruitment of 53BP1 but not Rad51 focus formation, and reduces BrdU-labeled ssDNA and RPA immunostaining, indicating a role in early 5′-3′ end resection. siRNA knockdown, comet assay, HR reporter assay, BrdU/RPA immunofluorescence Molecular and cellular biology High 21947284
2011 The human INO80 complex is organized in three modules assembling with distinct domains of hIno80 ATPase: (i) N-terminal domain with metazoan-specific subunits (dispensable for remodeling); (ii) HSA/PTH domain with Arp4, Arp8, and YY1; (iii) Snf2 ATPase domain with Ies2, Ies6, Arp5, Tip49a, and Tip49b. The evolutionarily conserved core catalyzes ATP-dependent nucleosome remodeling. Biochemical purification of subassemblies, in vitro ATPase and nucleosome remodeling assays The Journal of biological chemistry High 21303910
2011 Loss of Ies6 or Ino80 catalytic subunit causes polyploidy and chromosome missegregation; INO80 maintains normal chromatin structure at centromeres (pericentric chromatin) and prevents misincorporation of H2A.Z into pericentric regions. Genetic deletion, ploidy analysis, chromosome segregation assays, ChIP at centromeres Genes & development High 23207916
2012 Targeting INO80 to a chromatin locus via tethering enhances chromatin mobility in a manner requiring Ino80's ATPase activity; increased chromatin mobility correlates with increased rates of spontaneous gene conversion (homologous recombination). Live fluorescence microscopy, ATPase-dead mutant analysis, gene conversion assays Genes & development High 22345518
2013 Nuclear actin exists as a monomer within the yeast INO80 complex; its barbed end is not accessible for polymerization; a mutation in actin subdomain 2 reduces INO80 chromatin remodeling activity in vitro and impairs nuclear functions in vivo; subdomain 2 at the pointed end contributes to INO80's interaction with chromatin. Biochemical characterization, actin mutagenesis, in vitro chromatin remodeling assays, in vivo genetic assays Nature structural & molecular biology High 23524535
2013 Arp8 of INO80 interacts with nucleosomes principally via H3 and H4 histones through an insertion in the actin fold; Arp8 forms dimers that exploit the twofold symmetry of the nucleosome core for stable histone binding. Crystal structure of yArp8CTD, biochemical binding assays, electron microscopy Proceedings of the National Academy of Sciences of the United States of America High 23213201
2013 Ies2 is a potent activator of the intrinsic catalytic ATPase activity of the human Ino80 SNF2 ATPase; Ies6 and Arp5 together promote binding of the Ino80 ATPase to nucleosomes. In vitro ATPase assays with purified subassemblies, nucleosome binding assays Proceedings of the National Academy of Sciences of the United States of America High 24297934
2014 INO80 is recruited to replication forks in human cells through interaction with ubiquitinated H2A, aided by BAP1 (a nuclear deubiquitinase that also stabilizes Ino80 protein); Ino80 promotes fork progression under normal conditions; Ino80 is essential for mouse embryonic DNA replication. Co-immunoprecipitation, ChIP at replication forks (iPOND), DNA fiber assay, mouse knockout Nature communications High 25283999
2014 INO80 co-occupies pluripotency gene promoters with OCT4 and WDR5; its occupancy is dependent on OCT4 and WDR5; INO80 maintains open chromatin at pluripotency genes and licenses Mediator and RNA polymerase II recruitment for gene activation. ChIP-seq, ChIP-qPCR, RNAi depletion, ATAC/DNaseI accessibility assays, co-IP Cell stem cell High 24792115
2014 INO80 is required for DSB relocation to the inner nuclear membrane (Mps3) in S and G2 phases, requiring both INO80 activity and Rad51; this is distinct from DSB relocation to nuclear pores which is INO80-independent; SWR1-dependent H2A.Z incorporation is necessary for break relocation to either perinuclear site. Live-cell fluorescence microscopy, genetic epistasis with ino80, rad51, swr1 mutants Molecular cell High 25066231
2015 Mammalian INO80 removes H2A.Z from chromatin flanking DNA damage; the histone chaperone ANP32E similarly promotes HR and acts in the same pathway as INO80; HR defects in INO80- or ANP32E-depleted cells are rescued by co-depletion of H2A.Z, demonstrating that H2A.Z removal is the primary function of INO80 in promoting HR. RNAi depletion, ChIP, HR reporter assays, epistasis via H2A.Z co-depletion EMBO reports High 26142279
2015 INO80 complex interacts physically and functionally with Cdc48/p97/VCP to form a ternary complex with RNAPII; INO80 is required for turnover (degradation) of chromatin-bound ubiquitinated Rpb1 (largest RNAPII subunit) during transcriptional stress; cells lacking INO80 accumulate ubiquitinated Rpb1 on chromatin. Co-immunoprecipitation (Ino80-Cdc48-RNAPII), RNAPII ubiquitination and degradation assays, chromatin fractionation Molecular cell High 26656161
2015 The Arp5-Ies6 subcomplex forms an abundant distinct module in vivo that stimulates INO80-mediated ATPase and nucleosome sliding activity in vitro; Ies2 is required for Arp5-Ies6 association with the catalytic INO80 components; Arp5 insertion domains are required to couple ATP hydrolysis to nucleosome movement. Purification of Arp5-Ies6 subcomplex, in vitro ATPase and sliding assays, genetic and biochemical assembly analysis The Journal of biological chemistry High 26306040
2015 The Ino80 complex directly prevents euchromatin invasion into silent chromatin; Ino80C blocks H3K79 methylation by Dot1 in vitro; heterochromatin stimulates Ino80C binding in vitro and in vivo. In vitro H3K79 methylation blocking assay with purified Ino80C and Dot1, ChIP at heterochromatin borders, genetic analysis Genes & development High 25691465
2016 Inositol hexaphosphate (IP6) is a non-competitive inhibitor of the human INO80 complex that blocks the stimulatory effect of nucleosomes on ATPase activity; the IP6 binding site resides in the C-terminal region of the Ino80 subunit; Ies2 and Arp5/Ies6 regulate coupling of ATP hydrolysis to nucleosome sliding synergistically. In vitro ATPase and nucleosome sliding assays with purified recombinant hINO80; IP6 inhibition kinetics; subunit deletion analysis Nucleic acids research High 27257055
2016 INO80 occupies >90% of superenhancers in melanoma; Ino80 binding reduces nucleosome occupancy and facilitates Mediator recruitment at superenhancers; occupancy is dependent on transcription factors MITF and Sox9. ChIP-seq, ATAC-seq, Co-IP, RNAi knockdown with transcriptional and tumor growth readouts Genes & development High 27340176
2016 Mec1, INO80, and PAF1 complexes cooperate to degrade chromatin-bound RNAPII under replication stress; Mec1 triggers removal of PAF1C and RNAPII from transcribed genes near early firing origins during hydroxyurea treatment; failure to evict RNAPII correlates with replication fork restart defects. Genetic and proteomic analyses, ChIP of RNAPII/PAF1 in ino80 and mec1 mutants, replication restart assays Genes & development High 26798134
2017 INO80 translocates along DNA at the H2A-H2B interface of nucleosomes (not at the H3-H4 interface as other remodelers), creating persistent DNA displacement and torsional strain near the nucleosome entry site; this mechanism promotes both nucleosome mobilization and selective exchange of H2A.Z-H2B dimers for H2A-H2B without additional histone chaperones; INO80 mobilizes H2A.Z-containing nucleosomes more efficiently than H2A-containing ones. Site-directed protein-DNA crosslinking to map translocation site, in vitro histone exchange assay without chaperones, nucleosome sliding assays comparing H2A and H2A.Z substrates Nature communications High 28604691
2017 INO80 complex has at least two distinct functions during homologous recombination: DNA end resection and presynaptic filament formation; the second function is linked to H2A.Z—in the absence of H2A.Z, presynaptic filament formation and HR are restored in INO80-deficient cells. High-resolution HR assay in yeast, genetic epistasis with H2A.Z deletion, fluorescence microscopy of Rad51 foci Cell reports High 28514650
2017 Cryo-EM structure of human INO80 shows the complex is assembled around a single RUVBL1/RUVBL2 AAA+ heterohexamer; a spoked-wheel structural domain of Ino80 is engulfed by this heterohexamer; a cleft in RUVBL1/RUVBL2 forms a major interaction site for partner proteins. Cryo-EM structural analysis at 9.6 Å (portions at 4.1 Å) Nature structural & molecular biology High 29323271
2017 Two INO80 complexes cooperate during nucleosome spacing via dimerization of the Ino80CTD; a single active ATPase motor within the dimer is sufficient for sliding; ATPase activity is not regulated per se but becomes uncoupled as sliding reaches an endpoint, controlled by Ino80CTD. Biochemical reconstitution of dimer, ATPase assays, nucleosome sliding assays with wild-type vs. dead ATPase mutants eLife High 28585918
2017 A domain in Ino80 ATPase subunit (Ino80INS) stimulates Rvb1/Rvb2 ATPase activity 16-fold and promotes their dodecamerization; Ino80INS binds asymmetrically along the dodecamerization interface; ATP addition collapses dodecamers into hexamers, suggesting Rvbs act as protein assembly chaperones. In vitro ATPase stimulation assay, cryo-EM, mass spectrometry, integrative modeling Cell reports High 28591576
2018 Cryo-EM structure of the evolutionarily conserved INO80 core from Chaetomium thermophilum bound to a nucleosome at 3.7-4.3 Å reveals: Rvb1/Rvb2 heterohexamer acts as a stator scaffold; the Swi2/Snf2 ATPase motor binds nucleosomal DNA at SHL -6, unwraps ~15 bp, and disrupts H2A-DNA contacts; Arp5 and Ies6 bind SHL -2/-3 as a counter-grip; the Arp5 insertion domain (grappler) connects Arp5 actin-fold and entry DNA to pack against H2A-H2B acidic patch. Cryo-EM structure at 3.7-4.3 Å resolution with functional biochemical validation Nature High 29643509
2018 Cryo-EM structure of human INO80 with bound nucleosome reveals that the motor domains are located on entry DNA rather than at SHL2 (as in other remodelers); the ARP5-IES6 module contacts the opposite side of the nucleosome; H3 histone tails regulate INO80 motor domain activity (unlike other remodelers regulated by H4 tails). Cryo-EM structural analysis at 9.6 Å with 4.1 Å local resolution; functional ATPase assays with H3 tail mutants Nature High 29643506
2018 Crystal structure of the 180-kDa Arp8 module of yeast INO80 shows Arp8 engages nuclear actin distinctly from other actin-fold proteins; the HSA domain of Ino80 (spanning >120 Å) recruits the Arp4-N-actin heterodimer and provides an extended platform for extranucleosomal entry DNA required for nucleosome sliding and genome-wide nucleosome positioning. Crystal structure of Arp8 module; biochemical DNA binding assays; genome-wide nucleosome mapping in Arp8 mutants Nature structural & molecular biology High 30177756
2018 INO80 acts as a DNA length-sensitive switch: nucleosome sliding rate increases ~100-fold when flanking DNA increases from 40 to 60 bp; the Nhp10 module plays an auto-inhibitory role tuning this switch-like response; once initiated, INO80 moves nucleosomes processively at least 20 bp and can change direction without dissociation. Ensemble and single-molecule enzymology (FRET, single-molecule imaging), ATPase assays Molecular cell High 29452642
2018 The Arp8 N-terminus, Arp4 C-terminus, and Ino80 HSA domain bind extranucleosomal DNA 37-51 bp from the nucleosome edge, acting as a DNA-length sensor; disruption of Arp8/Arp4 DNA binding uncouples ATP hydrolysis from nucleosome mobilization by disengaging Arp5 from the H2A-H2B acidic patch and the Ino80-ATPase from SHL-6. Protein-DNA crosslinking, mutagenesis of DNA-binding interfaces, in vitro nucleosome sliding and ATPase assays Nature communications High 30120252
2019 TRIM3 E3 ubiquitin ligase mediates degradation of INO80 in the nucleus accumbens; TRIM3-INO80 interaction is reduced during cocaine abstinence (day 30), leading to increased INO80 protein levels that regulate transcriptional programs associated with cocaine craving. Co-immunoprecipitation (TRIM3-INO80), viral gene transfer, ChIP-seq Science advances Medium 31633032
2019 BAP1 promotes restart of hydroxyurea-induced stalled replication forks by stabilizing Ino80 and recruiting INO80 to stalled forks; ectopic INO80 expression rescues the fork restart defect of BAP1-depleted cells. RNAi depletion, DNA fiber assay, ChIP at stalled forks, rescue by INO80 overexpression The Biochemical journal High 31657441
2020 INO80 promotes resolution of R-loops to prevent replication-associated DNA damage in cancer cells; INO80 depletion increases R-loops; overexpression of RNase H1 rescues DNA synthesis defects from INO80 depletion; R-loops co-localize with and promote INO80 recruitment; artificial INO80 tethering enables R-loop turnover in cis. siRNA depletion, R-loop immunofluorescence (S9.6 antibody), DNA fiber assay, RNase H1 rescue, LacO tethering assay Nature communications High 32913330
2020 Linc-MYH lncRNA regulates the composition of the INO80 complex in muscle stem cells, preventing interaction of INO80 with WDR5 and YY1, selectively inhibiting the pro-proliferative function of INO80 without affecting its role in genome stability. RNA immunoprecipitation, Co-IP showing INO80-WDR5-YY1 interaction is blocked by linc-MYH, genetic deletion The EMBO journal Medium 32960481
2021 INO80 reads genomic information through DNA shape/mechanics encoded motifs, processing this through allosteric interplay between its core and Arp8 modules to position nucleosomes; at promoters, INO80 integrates DNA shape readout with general regulatory factor binding for +1 nucleosome positioning. Whole-genome chromatin reconstitution assays, biochemical analysis of allosteric module communication Nature communications High 34050142
2021 In primed pluripotent stem cells, INO80 promotes H2A.Z occupancy (deposition) at bivalent promoters and facilitates H3K27me3 installation and maintenance, leading to repression of developmental genes—an unexpected function opposite to INO80's known H2A.Z removal activity. Conditional Ino80 deletion, ChIP-seq for H2A.Z, H3K4me3, H3K27me3, gene expression analysis Nucleic acids research High 34139016
2022 Cryo-EM structures reveal how INO80 binds and is regulated by extranucleosomal DNA: the A-module (Arp8 regulatory module) binds linker DNA and is connected to the motor via an HSA/post-HSA lever that chemomechanically couples motor activity to linker DNA sensing; two sites of curved DNA recognition coordinate sliding regulation by extranucleosomal DNA; YY1/Ies4 subunit recruitment mechanism is revealed. Cryo-EM structural analysis of multiple INO80 states; functional sliding assays Science advances High 36490333
2022 INO80 prefers hexasomes (nucleosomes lacking one H2A-H2B dimer) as substrates over full nucleosomes by up to ~60-fold when flanking DNA approaches ~18-bp linkers; INO80 affects hexasome positioning within yeast genes in vivo; INO80 may promote nucleosome sliding by transiently dislodging H2A-H2B to make nucleosomes resemble hexasomes. In vitro sliding assays comparing nucleosome and hexasome substrates; in vivo MNase-seq in ino80 mutants Molecular cell High 35597239
2023 Cryo-EM structure of INO80-hexasome complex reveals that INO80 recognizes hexasome-specific DNA and histone features; loss of H2A-H2B triggers a large structural rearrangement (spin-rotated catalytic core) while the nuclear actin module remains tethered to unwrapped linker DNA; exposed H3-H4 interface directly activates INO80 independently of the H2A-H2B acidic patch. Cryo-EM structure of INO80-hexasome complex; functional ATPase and sliding assays Science (New York, N.Y.) High 37384673
2023 TORC1 activates Rpd3L histone deacetylase complex to deacetylate Ino80 at K929, protecting Ino80 from autophagy-mediated degradation; stabilized Ino80 then promotes H2A.Z eviction from autophagy-related gene promoters to repress their transcription; Rpd3L also deacetylates H2A.Z to block its chromatin deposition. Mass spectrometry identification of acetylation site, site-directed mutagenesis, ChIP, autophagy assays, rapamycin treatment Science advances High 36888706
2014 The mammalian INO80 complex is required for replication stress recovery: INO80 is specifically needed for replication elongation (not initiation); Ino80 or Arp8 depletion impairs replication restart after hydroxyurea treatment and causes DSB accumulation; INO80 protects stalled forks from collapse. siRNA depletion, DNA fiber labeling, γH2AX and Rad51 focus formation assays, comet assay Nucleic acids research High 25016522
2007 INO80 functions in sister chromatid cohesion: Arp8 mutant cells defective in INO80 chromatin remodeling show sister chromatid cohesion defects; Ino80 directly associates with centromeres and cohesin-associated regions; in early S phase, Ino80 is recruited to replication forks with Ctf18 and PCNA; arp8 mutation impairs Ctf18 and PCNA association with forks. ChIP at centromeres and cohesin sites, cohesion assay, co-localization with Ctf18 and PCNA by ChIP Cell cycle (Georgetown, Tex.) Medium 17471029

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2004 Recruitment of the INO80 complex by H2A phosphorylation links ATP-dependent chromatin remodeling with DNA double-strand break repair. Cell 484 15607975
2004 INO80 and gamma-H2AX interaction links ATP-dependent chromatin remodeling to DNA damage repair. Cell 448 15607974
2011 Global regulation of H2A.Z localization by the INO80 chromatin-remodeling enzyme is essential for genome integrity. Cell 337 21241891
2007 Distinct roles for SWR1 and INO80 chromatin remodeling complexes at chromosomal double-strand breaks. The EMBO journal 253 17762868
2009 Chromatin remodelling beyond transcription: the INO80 and SWR1 complexes. Nature reviews. Molecular cell biology 243 19424290
2005 A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex. The Journal of biological chemistry 195 16230350
2018 Structural basis for ATP-dependent chromatin remodelling by the INO80 complex. Nature 194 29643509
2007 A YY1-INO80 complex regulates genomic stability through homologous recombination-based repair. Nature structural & molecular biology 169 18026119
2007 YY1 functions with INO80 to activate transcription. Nature structural & molecular biology 161 17721549
2006 Interplay between Ino80 and Swr1 chromatin remodeling enzymes regulates cell cycle checkpoint adaptation in response to DNA damage. Genes & development 156 16951256
2008 The INO80 chromatin remodeling complex in transcription, replication and repair. Trends in biochemical sciences 152 19062292
2008 Ino80 chromatin remodeling complex promotes recovery of stalled replication forks. Current biology : CB 151 18406137
2018 Structure and regulation of the human INO80-nucleosome complex. Nature 150 29643506
2014 INO80 facilitates pluripotency gene activation in embryonic stem cell self-renewal, reprogramming, and blastocyst development. Cell stem cell 140 24792115
2014 SWR1 and INO80 chromatin remodelers contribute to DNA double-strand break perinuclear anchorage site choice. Molecular cell 139 25066231
2008 The Ino80 chromatin-remodeling enzyme regulates replisome function and stability. Nature structural & molecular biology 134 18376411
2012 Targeted INO80 enhances subnuclear chromatin movement and ectopic homologous recombination. Genes & development 130 22345518
2008 Distinct modes of regulation of the Uch37 deubiquitinating enzyme in the proteasome and in the Ino80 chromatin-remodeling complex. Molecular cell 126 18922472
2015 Removal of H2A.Z by INO80 promotes homologous recombination. EMBO reports 121 26142279
2017 INO80 exchanges H2A.Z for H2A by translocating on DNA proximal to histone dimers. Nature communications 117 28604691
1999 The product of the SNF2/SWI2 paralogue INO80 of Saccharomyces cerevisiae required for efficient expression of various yeast structural genes is part of a high-molecular-weight protein complex. Molecular microbiology 116 10361278
2004 The INO80 protein controls homologous recombination in Arabidopsis thaliana. Molecular cell 113 15525519
2010 The INO80 ATP-dependent chromatin remodeling complex is a nucleosome spacing factor. Molecular and cellular biology 110 21135121
2017 The INO80 remodeller in transcription, replication and repair. Philosophical transactions of the Royal Society of London. Series B, Biological sciences 105 28847827
2014 Stabilization and targeting of INO80 to replication forks by BAP1 during normal DNA synthesis. Nature communications 103 25283999
2013 Evidence for monomeric actin function in INO80 chromatin remodeling. Nature structural & molecular biology 103 23524535
2007 Mec1/Tel1 phosphorylation of the INO80 chromatin remodeling complex influences DNA damage checkpoint responses. Cell 101 17693258
2016 Mec1, INO80, and the PAF1 complex cooperate to limit transcription replication conflicts through RNAPII removal during replication stress. Genes & development 98 26798134
2011 Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. The Journal of biological chemistry 96 21303910
2014 INO80 and SWR complexes: relating structure to function in chromatin remodeling. Trends in cell biology 94 25088669
2013 Checkpoint kinases and the INO80 nucleosome remodeling complex enhance global chromatin mobility in response to DNA damage. Genes & development 93 24029917
2021 The INO80 chromatin remodeling complex promotes thermomorphogenesis by connecting H2A.Z eviction and active transcription in Arabidopsis. Molecular plant 91 34242850
2007 INO80 subfamily of chromatin remodeling complexes. Mutation research 89 17316710
2011 Mammalian Ino80 mediates double-strand break repair through its role in DNA end strand resection. Molecular and cellular biology 85 21947284
2010 INO80 chromatin remodeling complex promotes the removal of UV lesions by the nucleotide excision repair pathway. Proceedings of the National Academy of Sciences of the United States of America 84 20855601
2020 Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability. Nature communications 77 32913330
2018 Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease. Nature communications 77 29371594
2016 INO80 governs superenhancer-mediated oncogenic transcription and tumor growth in melanoma. Genes & development 75 27340176
2018 The nuclear actin-containing Arp8 module is a linker DNA sensor driving INO80 chromatin remodeling. Nature structural & molecular biology 66 30177756
2018 LncRNA PTCSC3 affects drug resistance of anaplastic thyroid cancer through STAT3/INO80 pathway. Cancer biology & therapy 65 29561707
2018 The Arp8 and Arp4 module acts as a DNA sensor controlling INO80 chromatin remodeling. Nature communications 64 30120252
2015 INO80 Chromatin Remodeler Facilitates Release of RNA Polymerase II from Chromatin for Ubiquitin-Mediated Proteasomal Degradation. Molecular cell 64 26656161
2015 Structural analyses of the chromatin remodelling enzymes INO80-C and SWR-C. Nature communications 63 25964121
2017 The INO80 Complex Removes H2A.Z to Promote Presynaptic Filament Formation during Homologous Recombination. Cell reports 62 28514650
2017 Cryo-EM structures of the human INO80 chromatin-remodeling complex. Nature structural & molecular biology 57 29323271
2019 INO80 and SWR1 complexes: the non-identical twins of chromatin remodelling. Current opinion in structural biology 56 31838293
2012 The INO80 chromatin remodeling complex prevents polyploidy and maintains normal chromatin structure at centromeres. Genes & development 56 23207916
2009 Cooperation between the INO80 complex and histone chaperones determines adaptation of stress gene transcription in the yeast Saccharomyces cerevisiae. Molecular and cellular biology 52 19620280
2009 INO80-dependent chromatin remodeling regulates early and late stages of mitotic homologous recombination. DNA repair 51 19095087
2010 Roles of human INO80 chromatin remodeling enzyme in DNA replication and chromosome segregation suppress genome instability. Cellular and molecular life sciences : CMLS 49 20237820
2010 The mammalian INO80 complex is recruited to DNA damage sites in an ARP8 dependent manner. Biochemical and biophysical research communications 49 20971067
2018 The Yeast INO80 Complex Operates as a Tunable DNA Length-Sensitive Switch to Regulate Nucleosome Sliding. Molecular cell 47 29452642
2014 INO80-C and SWR-C: guardians of the genome. Journal of molecular biology 47 25451604
2021 Genome information processing by the INO80 chromatin remodeler positions nucleosomes. Nature communications 45 34050142
2014 The mammalian INO80 chromatin remodeling complex is required for replication stress recovery. Nucleic acids research 45 25016522
2016 The INO80 Complex Requires the Arp5-Ies6 Subcomplex for Chromatin Remodeling and Metabolic Regulation. Molecular and cellular biology 44 26755556
2007 Regulation of telomere structure and functions by subunits of the INO80 chromatin remodeling complex. Molecular and cellular biology 44 17562861
2017 Regulation of Rvb1/Rvb2 by a Domain within the INO80 Chromatin Remodeling Complex Implicates the Yeast Rvbs as Protein Assembly Chaperones. Cell reports 43 28591576
2012 Interactions between the nucleosome histone core and Arp8 in the INO80 chromatin remodeling complex. Proceedings of the National Academy of Sciences of the United States of America 43 23213201
2010 The Ino80 chromatin-remodeling complex restores chromatin structure during UV DNA damage repair. The Journal of cell biology 43 21135142
2013 Multiple modes of regulation of the human Ino80 SNF2 ATPase by subunits of the INO80 chromatin-remodeling complex. Proceedings of the National Academy of Sciences of the United States of America 42 24297934
2005 Anc1 interacts with the catalytic subunits of the general transcription factors TFIID and TFIIF, the chromatin remodeling complexes RSC and INO80, and the histone acetyltransferase complex NuA3. Biochemical and biophysical research communications 41 15896708
2009 Fission yeast Iec1-ino80-mediated nucleosome eviction regulates nucleotide and phosphate metabolism. Molecular and cellular biology 40 19933844
2016 INO80 is required for oncogenic transcription and tumor growth in non-small cell lung cancer. Oncogene 39 27641337
2015 The Mouse INO80 Chromatin-Remodeling Complex Is an Essential Meiotic Factor for Spermatogenesis. Biology of reproduction 39 26607718
2023 Hexasome-INO80 complex reveals structural basis of noncanonical nucleosome remodeling. Science (New York, N.Y.) 38 37384673
2011 The INO80 family of chromatin-remodeling enzymes: regulators of histone variant dynamics. Cold Spring Harbor symposia on quantitative biology 38 21502417
2010 Human INO80 chromatin-remodelling complex contributes to DNA double-strand break repair via the expression of Rad54B and XRCC3 genes. The Biochemical journal 37 20687897
2015 The Ino80 complex prevents invasion of euchromatin into silent chromatin. Genes & development 36 25691465
2016 Synergy and antagonism in regulation of recombinant human INO80 chromatin remodeling complex. Nucleic acids research 33 27257055
2022 A hexasome is the preferred substrate for the INO80 chromatin remodeling complex, allowing versatility of function. Molecular cell 32 35597239
2022 Structural mechanism of extranucleosomal DNA readout by the INO80 complex. Science advances 32 36490333
2019 Roles of the INO80 and SWR1 Chromatin Remodeling Complexes in Plants. International journal of molecular sciences 32 31533258
2012 The RSC and INO80 chromatin-remodeling complexes in DNA double-strand break repair. Progress in molecular biology and translational science 32 22749148
2007 The INO80 chromatin remodeling complex functions in sister chromatid cohesion. Cell cycle (Georgetown, Tex.) 32 17471029
2014 An inherited immunoglobulin class-switch recombination deficiency associated with a defect in the INO80 chromatin remodeling complex. The Journal of allergy and clinical immunology 31 25312759
2018 The INO80 chromatin remodeler sustains metabolic stability by promoting TOR signaling and regulating histone acetylation. PLoS genetics 30 29462149
2018 INO80 Chromatin Remodeling Coordinates Metabolic Homeostasis with Cell Division. Cell reports 29 29346761
2015 Assembly of the Arp5 (Actin-related Protein) Subunit Involved in Distinct INO80 Chromatin Remodeling Activities. The Journal of biological chemistry 29 26306040
2007 The INO80 complex is required for damage-induced recombination. Biochemical and biophysical research communications 29 17320816
2020 Linc-MYH configures INO80 to regulate muscle stem cell numbers and skeletal muscle hypertrophy. The EMBO journal 28 32960481
2019 BAP1 promotes stalled fork restart and cell survival via INO80 in response to replication stress. The Biochemical journal 28 31657441
2006 Purification and assay of the human INO80 and SRCAP chromatin remodeling complexes. Methods (San Diego, Calif.) 28 17101442
2019 Ubiquitin-proteasomal regulation of chromatin remodeler INO80 in the nucleus accumbens mediates persistent cocaine craving. Science advances 27 31633032
2016 Distinct roles of the histone chaperones NAP1 and NRP and the chromatin-remodeling factor INO80 in somatic homologous recombination in Arabidopsis thaliana. The Plant journal : for cell and molecular biology 27 27352805
2020 Symmetric neural progenitor divisions require chromatin-mediated homologous recombination DNA repair by Ino80. Nature communications 26 32737294
2017 Crosstalk within a functional INO80 complex dimer regulates nucleosome sliding. eLife 24 28585918
2017 Genome maintenance functions of the INO80 chromatin remodeller. Philosophical transactions of the Royal Society of London. Series B, Biological sciences 24 28847826
2021 INO80 promotes H2A.Z occupancy to regulate cell fate transition in pluripotent stem cells. Nucleic acids research 23 34139016
2020 The INO80 Complex Regulates Epigenetic Inheritance of Heterochromatin. Cell reports 23 33378674
2004 In silico characterization of the INO80 subfamily of SWI2/SNF2 chromatin remodeling proteins. Biochemical and biophysical research communications 23 15207721
2016 Ino80 is essential for proximal-distal axis asymmetry in part by regulating Bmp4 expression. BMC biology 22 26975355
2015 Negative Regulation of p21Waf1/Cip1 by Human INO80 Chromatin Remodeling Complex Is Implicated in Cell Cycle Phase G2/M Arrest and Abnormal Chromosome Stability. PloS one 22 26340092
2018 Identification of Two Distinct Classes of the Human INO80 Complex Genome-Wide. G3 (Bethesda, Md.) 21 29432129
2005 Characterization of a human SWI2/SNF2 like protein hINO80: demonstration of catalytic and DNA binding activity. Biochemical and biophysical research communications 21 16298340
2023 The TORC1 activates Rpd3L complex to deacetylate Ino80 and H2A.Z and repress autophagy. Science advances 20 36888706
2018 Chromatin Remodeling Factors Isw2 and Ino80 Regulate Chromatin, Replication, and Copy Number of the Saccharomyces cerevisiae Ribosomal DNA Locus. Genetics 20 30355728
2016 Human INO80/YY1 chromatin remodeling complex transcriptionally regulates the BRCA2- and CDKN1A-interacting protein (BCCIP) in cells. Protein & cell 20 27535137
2016 miR-148a inhibits self-renewal of thyroid cancer stem cells via repressing INO80 expression. Oncology reports 20 27779717
2017 Global Analysis of SUMO-Binding Proteins Identifies SUMOylation as a Key Regulator of the INO80 Chromatin Remodeling Complex. Molecular & cellular proteomics : MCP 19 28254775