Affinage

IMMT

MICOS complex subunit MIC60 · UniProt Q16891

Length
758 aa
Mass
83.7 kDa
Annotated
2026-06-10
100 papers in source corpus 22 papers cited in narrative 22 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

IMMT (Mic60/mitofilin) is a coiled-coil inner mitochondrial membrane protein that resides at the mitochondrial periphery and intermembrane space and serves as the core scaffold of the MICOS complex organizing cristae junctions (PMID:8886976, PMID:26250910). Mic60 nucleates MICOS assembly—its loss destabilizes other MICOS subunits and disassembles the complex—through a direct interaction with Mic19/CHCHD3 that mediates mutual stabilization (PMID:26250910). By bridging to the outer membrane through a Sam50–Mic19–Mic60 axis, it integrates the SAM and MICOS complexes into the MIB supercomplex that establishes inner–outer membrane contact sites at cristae junctions, a configuration whose disruption (e.g., by OMA1-mediated Mic19 cleavage) collapses cristae junctions and lowers ATP production (PMID:31097788, PMID:28808085). Mic60 cooperates with OPA1, which is epistatic to Mic60 in setting cristae width and junction diameter, to control cristae shape (PMID:27974214), and it links cristae architecture to mtDNA function by interacting with TFAM and TFB2M and shaping the mtDNA D-loop to support mitochondrial transcription (PMID:25612828). Its abundance and activity are tuned by post-translational control: K48-linked ubiquitination at Lys285 by MARCH5 (antagonized by TRAP1 binding) and by MDM2 drives its degradation (PMID:37679468, PMID:37315416), while reciprocal phosphorylation governs cristae and mitophagy—PINK1 phosphorylates Mic60 on threonine residues to stabilize oligomerization and crista junction formation, whereas PKA phosphorylation at Ser528 negatively regulates PINK1–Parkin mitophagy (PMID:27153535, PMID:29456190, PMID:36708866). Mic60 is essential in vivo: inducible Immt deletion in adult mice disrupts MICOS and is lethal within 12 days, and homozygous IMMT variants cause a mitochondrial developmental encephalopathy with optic neuropathy through defective cristae organization (PMID:38467404, PMID:34842280).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1996 High

    Established the basic identity and topology of mitofilin, answering where this protein sits before any functional role was known.

    Evidence Subcellular fractionation, immunoelectron microscopy, and limited proteolysis localizing the coiled-coil protein to the inner membrane/intermembrane space periphery

    PMID:8886976

    Open questions at the time
    • No molecular function assigned
    • No interaction partners identified
    • Cristae role not yet established
  2. 2015 High

    Defined Mic60 as the core MICOS scaffold and linked cristae architecture to mtDNA transcription, moving from a structural protein to an organizing hub.

    Evidence shRNA knockdown with reciprocal Co-IP (Mic19/CHCHD3), EM morphology, mtDNA transcription assays, and TFAM/TFB2M binding plus mtDNA-3C in cultured cells

    PMID:25612828 PMID:26250910

    Open questions at the time
    • Mechanism connecting cristae scaffold to D-loop architecture not resolved
    • Yme1L regulation of Mic60 not mechanistically dissected
    • TFAM/POLRMT recruitment causality unclear
  3. 2016 High

    Placed Mic60 within the cristae-shaping hierarchy by showing OPA1 is epistatic, and connected it to mitochondrial dynamics and neuronal survival.

    Evidence Native-complex proteomics, reciprocal Co-IP, double-knockdown epistasis, electron tomography (OPA1 axis); plus respirometry and cell-death assays in dopaminergic neuronal models

    PMID:27001148 PMID:27974214

    Open questions at the time
    • Biochemical basis of OPA1–MIC60 epistasis unresolved
    • Direct vs indirect control of fission/fusion not distinguished
  4. 2016 High

    Identified phosphoregulation of Mic60 as a brake on PINK1–Parkin mitophagy, the first PTM control point on this scaffold.

    Evidence Mass-spectrometric phosphosite mapping (Ser528), phospho-mimic/dead mutagenesis with Parkin recruitment rescue, and Co-IP for transient PINK1 interaction

    PMID:27153535

    Open questions at the time
    • How Ser528 phosphorylation alters MIC60 structure unknown
    • PKA upstream signaling context not defined
  5. 2017 High

    Resolved the nanoscale distribution of Mic60/Mic19/Sam50 to cristae junctions, providing physical grounding for the membrane-bridging model.

    Evidence Genetically encoded EM tags (miniSOG, APEX2) with electron tomography

    PMID:28808085

    Open questions at the time
    • Functional meaning of intra-crista enrichment unclear
    • Stoichiometry at junctions not quantified
  6. 2018 High

    Established a direct PINK1 kinase–MIC60 substrate relationship that stabilizes oligomerization and crista junctions, with in vivo and disease relevance.

    Evidence Drosophila genetics, biochemical phosphorylation reconstitution, oligomerization assay, EM of crista junctions, rescue of PINK1-null phenotypes, and human neuron studies plus PD patient variants

    PMID:29456190

    Open questions at the time
    • Phosphorylated threonine residues not all mapped
    • How phosphorylation drives oligomerization mechanistically unresolved
  7. 2019 High

    Defined the Sam50–Mic19–Mic60 axis as the structural bridge integrating SAM and MICOS into the MIB supercomplex linking the two mitochondrial membranes.

    Evidence Reciprocal Co-IP/pulldown, knockdown, super-resolution and electron microscopy, OMA1-cleavage dissection, and ATP assays

    PMID:31097788

    Open questions at the time
    • Direct Mic60–Sam50 contact vs Mic19-bridged contact not fully separated
    • Regulation of OMA1 cleavage in physiology unclear
  8. 2017 Medium

    Extended Mic60 function beyond cristae to mitochondrial transport, showing it controls Miro levels and mitochondrial motility in neurons.

    Evidence Drosophila loss-of-function genetics, Western blotting of Miro, motility assays, and neuromuscular junction imaging

    PMID:28904209

    Open questions at the time
    • Mechanism by which MIC60 regulates Miro abundance unknown
    • Whether this is conserved in mammals not tested
  9. 2021 Medium

    Identified ubiquitin-dependent control of MIC60 stability, with TRAP1 protecting MIC60 to preserve mitochondrial integrity under acidosis.

    Evidence Co-IP for TRAP1–MIC60, knockdown/overexpression with membrane potential and ATP readouts in cardiac injury models

    PMID:34907169

    Open questions at the time
    • E3 ligase identity not defined in this study
    • Direct vs indirect protection by TRAP1 unclear
  10. 2023 High

    Mapped MARCH5 as a site-specific E3 ligase ubiquitinating MIC60 at Lys285, with TRAP1 acting as a competitive antagonist controlling MIC60 turnover.

    Evidence Co-IP, ubiquitination assays, site-directed mutagenesis, mass spectrometry, and knockdown under diabetic conditions

    PMID:37679468

    Open questions at the time
    • Trigger linking metabolic stress to MARCH5 activation unclear
    • Relationship of MARCH5 pathway to MDM2 pathway not reconciled
  11. 2023 Medium

    Identified MDM2 as a second E3 ligase degrading MIC60, linking its loss to impaired mitophagy and microglial inflammation.

    Evidence In vivo mouse and in vitro ZnO-NP models, siRNA knockdown, ubiquitination and mitophagy assays

    PMID:37315416

    Open questions at the time
    • MDM2 ubiquitination site on MIC60 not mapped
    • Overlap/competition with MARCH5 unresolved
  12. 2023 Medium

    Demonstrated organismal dependence on Mic60 dosage, showing haploinsufficiency drives age-dependent neurodegeneration through redox-sensitive mitochondrial dysfunction.

    Evidence Mic60 heterozygous mouse model with EM, membrane potential, ATP, ROS, and OXPHOS assays plus N-acetylcysteine rescue

    PMID:36974636

    Open questions at the time
    • Causal step from cristae defect to neuronal loss not isolated
    • Why effects are age-dependent unexplained
  13. 2024 High

    Proved Mic60 is essential for adult mitochondrial integrity, establishing that acute MICOS loss is rapidly lethal.

    Evidence Tamoxifen-inducible conditional knockout mouse with EM, Western blotting, and pathology

    PMID:38467404

    Open questions at the time
    • Tissue-specific contributions to lethality not parsed
    • Molecular cause of death sequence unresolved
  14. 2024 Medium

    Showed MIC60 is hijacked by pathogens, with Listeria LLO binding it to trigger fission and promote infection.

    Evidence Co-IP, LLO Phe251 mutagenesis, F-actin tail and mitochondrial morphology imaging in infection models

    PMID:39431455

    Open questions at the time
    • How LLO binding triggers fission mechanistically unclear
    • Single Co-IP-based interaction model
  15. 2025 Medium

    Identified lactylation as a metabolic PTM on Mic60 that remodels cristae and drives therapy resistance, linking cristae architecture to cancer metabolism.

    Evidence Anoikis-resistant and metastasis models, protein lactylation assay, cristae morphology and metabolic profiling, with DAPK2 modulation

    PMID:40812308

    Open questions at the time
    • Lactylation sites on Mic60 not mapped in abstract
    • Mechanism linking DAPK2 to Mic60 lactylation unclear
  16. 2025 Medium

    Used ancestral reconstruction to pinpoint the C-terminal Mitofilin domain as the determinant of respiratory function across eukaryotic lineages.

    Evidence Ancestral sequence reconstruction with yeast complementation and comparative mutagenesis

    PMID:40545685

    Open questions at the time
    • Molecular activity of the four key residues not defined
    • Link to specific MICOS or respiratory partner unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the multiple competing post-translational modifications (MARCH5/MDM2 ubiquitination, PINK1/PKA phosphorylation, lactylation) are integrated to set MIC60 abundance and cristae state in a given physiological context remains unresolved.
  • No unified model reconciling the PTM control points
  • Structural basis of MIC60 scaffolding at atomic resolution lacking
  • Mammalian conservation of the motility/Miro role untested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0060090 molecular adaptor activity 2 GO:0003677 DNA binding 1
Localization
GO:0005739 mitochondrion 3
Pathway
R-HSA-1852241 Organelle biogenesis and maintenance 4 R-HSA-9612973 Autophagy 3 R-HSA-8953854 Metabolism of RNA 1
Partners
MARCH5MIC19/CHCHD3OPA1PINK1SAMM50TFAMTFB2MTRAP1
Complex memberships
MIB supercomplexMICOSSAM complex (via Sam50-Mic19-Mic60 axis)

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1996 Mitofilin (IMMT/HMP) is a mitochondrial inner membrane protein that resides predominantly in the intermembrane space, contains predicted coiled-coil domains, is resistant to detergent extraction, co-purifies with mitochondria, and is not co-localized with Golgi or ER. Limited proteolysis and immuno-electron microscopy confirmed its intermembrane space localization at the mitochondrial periphery. Subcellular fractionation, immunofluorescence, immunoelectron microscopy, limited proteolysis, Western blotting Journal of cell science High 8886976
2015 Mic60/Mitofilin is a core scaffold for MICOS complex assembly: knockdown of Mic60 causes instability of other MICOS components and disassembly of the complex. Mic60 directly interacts with Mic19/CHCHD3, and this interaction is required for their mutual stabilization. Disrupted MICOS assembly causes giant mitochondria formation due to dysregulated fission/fusion, and mtDNA nucleoids become clustered and transcription is attenuated. The mitochondrial i-AAA protease Yme1L regulates Mic60 homeostasis. shRNA knockdown, co-immunoprecipitation, Western blotting, immunofluorescence, electron microscopy, mtDNA transcription assay Cell death and differentiation High 26250910
2016 MIC60 physically interacts with OPA1, and together they control cristae junction number and stability. OPA1 is epistatic to MIC60 in cristae shape regulation: OPA1 defines cristae width and junction diameter independently of MIC60, while both are components of high-molecular-weight complexes disrupted during cristae remodeling. MIC60 is a core MICOS protein required for normal cristae junction formation. Proteomics of native complexes, co-immunoprecipitation, genetic epistasis (double KD), electron tomography, biochemistry Cell reports High 27974214
2016 PKA phosphorylates MIC60 at Ser528. MIC60 transiently interacts with PINK1. MIC60 downregulation reduces PINK1 protein levels and causes mislocalization of Parkin. Phosphorylation-mimic MIC60 (Ser528Asp) fails to restore Parkin recruitment in MIC60-knockdown cells, while phosphorylation-deficient MIC60 (Ser528Ala) facilitates mitochondrial Parkin localization. Thus PKA-mediated phosphorylation of MIC60 negatively regulates the PINK1-Parkin mitophagy pathway. Mutational analysis, mass spectrometry, co-immunoprecipitation, shRNA knockdown, fluorescence microscopy Molecular cell High 27153535
2018 PINK1 phosphorylates the inner mitochondrial membrane protein MIC60/mitofilin in Drosophila and human neurons. PINK1-dependent phosphorylation stabilizes MIC60 oligomerization and promotes crista junction formation. Expression of MIC60 rescues crista structure, ATP levels, behavioral defects, and dopaminergic neurodegeneration in PINK1-null Drosophila. MIC60 coding variants in the mitochondrial targeting sequence found in PD patients impair crista junction formation in Drosophila. In vivo Drosophila genetics, biochemical reconstitution of phosphorylation, MIC60 oligomerization assay, electron microscopy of crista junctions, rescue experiments, human neuron studies Molecular cell High 29456190
2015 Mic60 interacts with mitochondrial transcription factors TFAM and TFB2M. Mic60 knockdown compromises mitochondrial transcription and OXPHOS activities, decreases TFAM binding and POLRMT recruitment to mtDNA promoters. Mic60 interacts with mtDNA and is involved in the architecture of the mtDNA D-loop region, as shown by mtDNA immunoprecipitation and 3C assays. Co-immunoprecipitation, shRNA knockdown, ChIP on mtDNA, mIP-3C assay, OXPHOS activity measurement Scientific reports Medium 25612828
2019 Mic19 directly interacts with both Sam50 (outer membrane SAM complex) and Mic60 (MICOS complex) to form a Sam50-Mic19-Mic60 axis that integrates SAM and MICOS into the MIB (mitochondrial intermembrane space bridging) supercomplex, mediating outer- and inner-membrane contact. OMA1-mediated cleavage of Mic19 disrupts this axis, causing MIB disassembly, loss of cristae junctions, and reduced ATP production. Sam50 acts as an anchoring point guiding cristae junction formation at the outer membrane. Co-immunoprecipitation, pulldown, knockdown, super-resolution microscopy, electron microscopy, ATP production assay Cell death and differentiation High 31097788
2017 Mic19, Mic60, and Sam50 localize specifically to crista junctions in a network pattern along the mitochondrial periphery, with enrichment also inside cristae, as determined by miniSOG and APEX2 genetic tags and electron tomography at nanoscale resolution. Sam50 is not uniformly distributed in the outer mitochondrial membrane and incompletely overlaps with Mic19/Mic60 at crista junctions. Genetically encoded electron microscopy tags (miniSOG, APEX2), electron tomography, immunofluorescence Journal of cell science High 28808085
2017 Drosophila MIC60 performs dual functions: (1) maintenance of crista membrane structure (canonical role) and (2) regulation of mitochondrial motility by influencing protein levels of the outer mitochondrial membrane protein Miro that anchors mitochondria to microtubule motors. Loss of MIC60 causes loss of Miro and mitochondrial arrest, and disrupts synaptic structure and function at neuromuscular junctions. Drosophila genetic mutant analysis, Western blotting, mitochondrial motility assays, neuromuscular junction imaging, electron microscopy Molecular biology of the cell Medium 28904209
2023 MARCH5 acts as an E3 ubiquitin ligase that specifically targets MIC60, mediating K48-linked ubiquitination at Lys285 to promote its degradation under high glucose/palmitate (diabetic) conditions. TRAP1 inhibits MARCH5-mediated ubiquitination by competitively binding to MIC60. Mutation of the MIC60 ubiquitination site (Lys285) or the MIC60-interacting motifs in MARCH5 abrogates MARCH5-mediated MIC60 ubiquitination and degradation. Co-immunoprecipitation, ubiquitination assay, site-directed mutagenesis, mass spectrometry, shRNA knockdown, overexpression Cell death and differentiation High 37679468
2021 TRAP1 mitigates extracellular acidosis-induced mitochondrial impairment and cardiac injury by directly interacting with MIC60 to decrease its ubiquitin-dependent degradation. Overexpression of MIC60 in acidosis maintains cell viability, increases ATP production, mitochondrial membrane potential, and mitigates disruption of mitochondrial structure. Co-immunoprecipitation, overexpression, shRNA knockdown, mitochondrial membrane potential assay, ATP measurement Cell death discovery Medium 34907169
2023 MDM2 acts as an E3 ubiquitin ligase that increases MIC60 ubiquitination, causing its degradation, which impairs mitophagy and induces autophagosome overaccumulation and microglial inflammation. MDM2 silencing significantly attenuates mitochondrial damage caused by ZnO-NPs by preventing MIC60 ubiquitination and degradation. In vivo mouse model, in vitro ZnO-NP exposure, siRNA knockdown, ubiquitination assay, mitophagy assay, immunofluorescence Journal of hazardous materials Medium 37315416
2024 Tamoxifen-inducible deletion of Immt in adult mice disrupts the MICOS complex, increases mitochondrial size, alters cristae morphology, and is lethal within 12 days, demonstrating that MIC60 is essential for MICOS integrity in vivo. Pathological consequences include defective intestinal muscle function (paralytic ileus) and bone marrow hypocellularity. Conditional knockout mouse model (ROSA-CreERT2), electron microscopy, Western blotting, pathological analysis Life science alliance High 38467404
2020 MIC60 was identified as the direct binding target of the small molecule miclxin using drug-immobilized affinity beads. MIC60 dysfunction caused by miclxin induces a mitochondrial stress response in a mutant β-catenin-dependent manner, leading to Bcl-2 downregulation, loss of mitochondrial membrane potential, and apoptosis-inducing factor-dependent apoptosis. Affinity bead pulldown (drug-immobilized beads), Western blotting, flow cytometry, cell death assays ACS chemical biology Medium 32584541
2016 Mic60/mitofilin overexpression in dopaminergic neuronal cells attenuates dopamine- and rotenone-induced cell death, increases mitochondrial respiration and spare respiratory capacity, suppresses mitochondrial fission, and increases mitochondrial length in neurites. Mic60 knockdown potentiates dopamine-induced cell death and suppresses respiration, demonstrating that Mic60 controls both mitochondrial membrane function and fission/fusion dynamics in neurons. shRNA knockdown, overexpression, Seahorse respirometry, mitochondrial imaging, cell death assays in differentiated SH-SY5Y and PC12 cells and primary rat cortical neurons Neurobiology of disease Medium 27001148
2023 Alphaproteobacterial Mic60 (ortholog of eukaryotic IMMT/Mic60) physically interacts with BamA, the homolog of Sam50, recapitulating the conserved Mic60-Sam50 interaction from bacteria to mitochondria. Disruption of Mic60 and adjacent gene Orf52 in Rhodobacter sphaeroides and Rhodopseudomonas palustris causes photoheterotrophic growth defects and enlarged intracytoplasmic membranes (ICMs), supporting a role in ICM/crista organization. Genetic disruption, overexpression, physical interaction assay, electron microscopy, photoheterotrophic growth assays Current biology : CB Medium 36921606
2021 Homozygous pathogenic variants (c.895A>G, p.Lys299Glu) in IMMT cause mitochondrial developmental encephalopathy with optic neuropathy in humans. Functional studies showed that loss of IMMT function causes alterations in mitochondrial cristae morphology, confirming the essential role of IMMT/mitofilin in cristae organization in vivo. Clinical genetics, Sanger sequencing, electron microscopy of patient mitochondria, co-segregation analysis Clinical genetics Medium 34842280
2025 Lactylation of Mic60 protein is a critical post-translational modification that affects mitochondrial cristae remodeling and activates mitochondrial metabolism. Dysfunction of DAPK2 kinase alters Mic60 protein in mitochondrial cristae, increasing cristae abundance and compactness and activating mitochondrial metabolism, contributing to EGFR-TKI resistance and cancer metastasis. Anoikis-resistant cell model, mouse tail vein metastasis model, protein lactylation assay, mitochondrial morphology analysis, metabolic profiling Developmental cell Medium 40812308
2023 Haploinsufficiency of Mic60 causes progressive neurological abnormalities with mitochondrial structural damage and neuronal loss in mice. Mic60 haploinsufficiency reduces mitochondrial membrane potential, cellular ATP production, increases ROS, and alters OXPHOS complexes in neurons in an age-dependent manner. Systemic N-acetylcysteine largely reverses mitochondrial dysfunction in haplo-insufficient Mic60 mice. Mic60 heterozygous mouse model, electron microscopy, mitochondrial membrane potential assay, ATP measurement, ROS assay, glucose brain imaging, Western blotting Brain pathology (Zurich, Switzerland) Medium 36974636
2024 L. monocytogenes virulence factor LLO directly interacts with Mic60 (via Phe251 of LLO) to trigger transient mitochondrial fission, enhancing bacterial pathogenicity. Mic60 affects the formation of F-actin tails recruited by L. monocytogenes. L. monocytogenes infection affects Mic60 expression, leading to changes in mitochondrial morphology, membrane potential, and ROS production. Co-immunoprecipitation, site-directed mutagenesis (Phe251), F-actin tail imaging, mitochondrial morphology assay, bacterial infection model Advanced science (Weinheim, Baden-Wurttemberg, Germany) Medium 39431455
2023 Carnosic acid prevents 6-OHDA-induced cytochrome c release by regulating Mic60 phosphorylation: PINK1 phosphorylates Mic60 on threonine residues (interaction decreased by 6-OHDA), while PKA phosphorylates Mic60 on serine residues. PINK1 siRNA and PKA activator (forskolin) reversed the Mic60 phosphorylation interactions. Mic60 and PINK1 siRNAs blocked carnosic acid's protective effect on cytochrome c release, establishing the PINK1-Mic60 axis in neuroprotection. Co-immunoprecipitation, siRNA knockdown, immunoprecipitation with phospho-specific antibodies, cytochrome c release assay Food and chemical toxicology Medium 36708866
2024 Ancestral sequence reconstruction of the Mic60 Mitofilin domain identified four specific residues sufficient to explain the respiratory functional difference between yeast-lineage and animal-derived Mitofilin ancestors. Yeast-lineage Mitofilin ancestors as far back as the last opisthokont common ancestor rescue respiration in yeast, demonstrating that the C-terminal Mitofilin domain is required for cellular respiration. Ancestral sequence reconstruction, yeast complementation/rescue assay, comparative mutagenesis Protein science Medium 40545685

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 NO sensing by FNR: regulation of the Escherichia coli NO-detoxifying flavohaemoglobin, Hmp. The EMBO journal 237 12093725
1991 Isolation and nucleotide sequence of the hmp gene that encodes a haemoglobin-like protein in Escherichia coli K-12. Molecular & general genetics : MGG 190 2034230
2006 Maintenance of nitric oxide and redox homeostasis by the salmonella flavohemoglobin hmp. The Journal of biological chemistry 154 16873371
2015 Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization. Cell death and differentiation 151 26250910
2016 Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control. Cell reports 149 27974214
1999 Anoxic function for the Escherichia coli flavohaemoglobin (Hmp): reversible binding of nitric oxide and reduction to nitrous oxide. FEBS letters 134 10094495
2000 Flavohemoglobin Hmp affords inducible protection for Escherichia coli respiration, catalyzed by cytochromes bo' or bd, from nitric oxide. The Journal of biological chemistry 133 10915782
2016 PKA Regulates PINK1 Stability and Parkin Recruitment to Damaged Mitochondria through Phosphorylation of MIC60. Molecular cell 107 27153535
1992 The haemoglobin-like protein (HMP) of Escherichia coli has ferrisiderophore reductase activity and its C-terminal domain shares homology with ferredoxin NADP+ reductases. FEBS letters 104 1601132
2018 PINK1 Phosphorylates MIC60/Mitofilin to Control Structural Plasticity of Mitochondrial Crista Junctions. Molecular cell 97 29456190
2019 Sam50-Mic19-Mic60 axis determines mitochondrial cristae architecture by mediating mitochondrial outer and inner membrane contact. Cell death and differentiation 92 31097788
1999 Regulation of hmp gene transcription in Mycobacterium tuberculosis: effects of oxygen limitation and nitrosative and oxidative stress. Journal of bacteriology 73 10348862
1998 A novel mechanism for upregulation of the Escherichia coli K-12 hmp (flavohaemoglobin) gene by the 'NO releaser', S-nitrosoglutathione: nitrosation of homocysteine and modulation of MetR binding to the glyA-hmp intergenic region. Molecular microbiology 71 9767577
1996 Molecular characterization of mitofilin (HMP), a mitochondria-associated protein with predicted coiled coil and intermembrane space targeting domains. Journal of cell science 70 8886976
1992 Activation of protein serine/threonine kinases p42, p63, and p87 in Rous sarcoma virus-transformed cells: signal transduction/transformation-dependent MBP kinases. Molecular biology of the cell 70 1337288
2009 Ras is an indispensable coregulator of the class IB phosphoinositide 3-kinase p87/p110gamma. Proceedings of the National Academy of Sciences of the United States of America 68 19906996
1996 The flavohaemoglobin (HMP) of Escherichia coli generates superoxide in vitro and causes oxidative stress in vivo. FEBS letters 64 8612736
1985 In situ analysis of antigens on malignant and benign cells of the melanocyte lineage. Differential expression of two surface molecules, gp75 and p89. The Journal of experimental medicine 61 3973533
2001 The divergent Caenorhabditis elegans beta-catenin proteins BAR-1, WRM-1 and HMP-2 make distinct protein interactions but retain functional redundancy in vivo. Genetics 59 11560894
1994 Reactions of the Escherichia coli flavohaemoglobin (Hmp) with oxygen and reduced nicotinamide adenine dinucleotide: evidence for oxygen switching of flavin oxidoreduction and a mechanism for oxygen sensing. Proceedings. Biological sciences 59 8022841
2001 Escherichia coli flavohaemoglobin (Hmp) with equistoichiometric FAD and haem contents has a low affinity for dioxygen in the absence or presence of nitric oxide. The Biochemical journal 55 11139382
2000 Spiroplasma citri Surface Protein P89 Implicated in Adhesion to Cells of the Vector Circulifer tenellus. Phytopathology 54 18944490
2015 Suppression of Mic60 compromises mitochondrial transcription and oxidative phosphorylation. Scientific reports 50 25612828
2007 The hmp gene encoding the NO-inducible flavohaemoglobin in Escherichia coli confers a protective advantage in resisting killing within macrophages, but not in vitro: links with swarming motility. Gene 49 17611046
2003 Molecular and structural characterization of the HMP-AB gene encoding a pore-forming protein from a clinical isolate of Acinetobacter baumannii. Current microbiology 43 14669924
2004 The structure and ligand binding properties of the B. subtilis YkoF gene product, a member of a novel family of thiamin/HMP-binding proteins. Journal of molecular biology 41 15451668
2013 p87 and p101 subunits are distinct regulators determining class IB phosphoinositide 3-kinase (PI3K) specificity. The Journal of biological chemistry 40 24014027
2014 Genetic characterization of the hmp locus, a chemotaxis-like gene cluster that regulates hormogonium development and motility in Nostoc punctiforme. Molecular microbiology 38 24533832
2017 Drosophila MIC60/mitofilin conducts dual roles in mitochondrial motility and crista structure. Molecular biology of the cell 35 28904209
2002 Manifold decreased protein levels of matrin 3, reduced motor protein HMP and hlark in fetal Down's syndrome brain. Proteomics 35 12469345
2018 Mitochondrial inner membrane protein, Mic60/mitofilin in mammalian organ protection. Journal of cellular physiology 34 30259514
2016 Mic60/mitofilin overexpression alters mitochondrial dynamics and attenuates vulnerability of dopaminergic cells to dopamine and rotenone. Neurobiology of disease 33 27001148
2003 Flavohemoglobin Hmp, but not its individual domains, confers protection from respiratory inhibition by nitric oxide in Escherichia coli. The Journal of biological chemistry 31 12826671
1992 Phosphorylation of the MARCKS protein (P87), a major protein kinase C substrate, is not an obligatory step in the mitogenic signaling pathway of basic fibroblast growth factor in rat oligodendrocytes. Journal of neurochemistry 31 1309563
2023 MDM2 upregulation induces mitophagy deficiency via Mic60 ubiquitination in fetal microglial inflammation and consequently neuronal DNA damage caused by exposure to ZnO-NPs during pregnancy. Journal of hazardous materials 29 37315416
1998 Expression of DNA excision-repair-cross-complementing proteins p80 and p89 in brain of patients with Down Syndrome and Alzheimer's disease. Neuroscience letters 28 9714461
1996 Reactions of the Escherichia coli flavohaemoglobin (Hmp) with NADH and near-micromolar oxygen: oxygen affinity of NADH oxidase activity. Microbiology (Reading, England) 28 8704956
2019 A bacterial riboswitch class for the thiamin precursor HMP-PP employs a terminator-embedded aptamer. eLife 27 30950790
2017 Sub-mitochondrial localization of the genetic-tagged mitochondrial intermembrane space-bridging components Mic19, Mic60 and Sam50. Journal of cell science 26 28808085
2012 The last piece in the vitamin B1 biosynthesis puzzle: structural and functional insight into yeast 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase. The Journal of biological chemistry 26 23048037
2006 Multiple regulators of the Flavohaemoglobin (hmp) gene of Salmonella enterica serovar Typhimurium include RamA, a transcriptional regulator conferring the multidrug resistance phenotype. Archives of microbiology 26 17024490
1997 Roles of RpoS (sigmaS), IHF and ppGpp in the expression of the hmp gene encoding the flavohemoglobin (Hmp) of Escherichia coli K-12. Molecular & general genetics : MGG 23 9197421
2021 Loss of MIC60 Aggravates Neuronal Death by Inducing Mitochondrial Dysfunction in a Rat Model of Intracerebral Hemorrhage. Molecular neurobiology 22 34232477
2019 Potential Role of Mic60/Mitofilin in Parkinson's Disease. Frontiers in neuroscience 22 30740041
2019 Expression of OPA1 and Mic60 genes and their association with mitochondrial cristae morphology in Tibetan sheep. Cell and tissue research 21 30612186
1998 Response of the NAD(P)H-oxidising flavohaemoglobin (Hmp) to prolonged oxidative stress and implications for its physiological role in Escherichia coli. FEMS microbiology letters 20 9770277
2025 Dapk2 dysfunction leads to Mic60 lactylation and mitochondrial metabolic reprogramming, promoting lung cancer EGFR-TKI resistance and metastasis. Developmental cell 19 40812308
2014 Carbon monoxide-releasing molecule-3 (CORM-3; Ru(CO)3Cl(glycinate)) as a tool to study the concerted effects of carbon monoxide and nitric oxide on bacterial flavohemoglobin Hmp: applications and pitfalls. The Journal of biological chemistry 19 25193663
2014 Cyclic dipeptides produced by fungus Eupenicillium brefeldianum HMP-F96 induced extracellular alkalinization and H2O 2 production in tobacco cell suspensions. World journal of microbiology & biotechnology 19 25344087
2011 The β-catenin HMP-2 functions downstream of Src in parallel with the Wnt pathway in early embryogenesis of C. elegans. Developmental biology 19 21575624
2023 TRAP1 inhibits MARCH5-mediated MIC60 degradation to alleviate mitochondrial dysfunction and apoptosis of cardiomyocytes under diabetic conditions. Cell death and differentiation 17 37679468
1995 Distribution of the flavohaemoglobin, HMP, between periplasm and cytoplasm in Escherichia coli. FEMS microbiology letters 17 7875569
2023 Multi-omics and experimental analysis unveil theragnostic value and immunological roles of inner membrane mitochondrial protein (IMMT) in breast cancer. Journal of translational medicine 16 36899366
2018 HMP-1/α-catenin promotes junctional mechanical integrity during morphogenesis. PloS one 16 29466456
2013 Corynebacterium glutamicum ArnR controls expression of nitrate reductase operon narKGHJI and nitric oxide (NO)-detoxifying enzyme gene hmp in an NO-responsive manner. Journal of bacteriology 15 24142248
1999 Inhibition of experimental autoimmune encephalomyelitis in SJL mice by oral administration of retro-inverso derivative of encephalitogenic epitope P87-99. European journal of immunology 15 10458771
2021 TRAP1 inhibits MIC60 ubiquitination to mitigate the injury of cardiomyocytes and protect mitochondria in extracellular acidosis. Cell death discovery 14 34907169
2013 Role of flavohaemoprotein Hmp and nitrate reductase NarGHJI of Corynebacterium glutamicum for coping with nitrite and nitrosative stress. FEMS microbiology letters 14 24237595
2010 HMP binding protein ThiY and HMP-P synthase THI5 are structural homologues. Biochemistry 14 20873853
2014 Cytotoxic polyphenols from a sponge-associated fungus Aspergillus versicolor Hmp-48. Chemistry & biodiversity 13 24443433
2018 Partial Inhibition of HO-1 Attenuates HMP-Induced Hepatic Regeneration against Liver Injury in Rats. Oxidative medicine and cellular longevity 12 29849924
2010 The TFIIH subunit p89 (XPB) localizes to the centrosome during mitosis. Cellular oncology : the official journal of the International Society for Cellular Oncology 12 20208140
2023 Mic60 is essential to maintain mitochondrial integrity and to prevent encephalomyopathy. Brain pathology (Zurich, Switzerland) 11 36974636
2012 Expression of p89(c-Mybex9b), an alternatively spliced form of c-Myb, is required for proliferation and survival of p210BCR/ABL-expressing cells. Blood cancer journal 11 22829973
2010 Effects of 3-(2-Hydroxyphenyl)-1-(5-methyl-furan-2-y-l) propenone (HMP) upon signalling pathways of lipopolysaccharide-induced iNOS synthesis in RAW 264.7 cells. International immunopharmacology 11 21035434
2023 Intracytoplasmic-membrane development in alphaproteobacteria involves the homolog of the mitochondrial crista-developing protein Mic60. Current biology : CB 10 36921606
2020 Miclxin, a Novel MIC60 Inhibitor, Induces Apoptosis via Mitochondrial Stress in β-Catenin Mutant Tumor Cells. ACS chemical biology 10 32584541
2011 Transcriptional profiling analysis of HMP-treated rats with experimentally induced myocardial infarction. Journal of ethnopharmacology 10 21605651
1993 Androgenic regulation of glycolytic and HMP pathway in epididymis and vas deferens of rhesus monkey. Indian journal of experimental biology 10 8359828
2023 Nitroxide-HMP-Protects Human Trophoblast HTR-8/SVneo Cells from H2O2-Induced Oxidative Stress by Reducing the HIF1A Signaling Pathway. Antioxidants (Basel, Switzerland) 9 37627573
2022 Improvement of the Antibacterial Activity of Phage Lysin-Derived Peptide P87 through Maximization of Physicochemical Properties and Assessment of Its Therapeutic Potential. Antibiotics (Basel, Switzerland) 9 36290106
2021 Mitochondrial developmental encephalopathy with bilateral optic neuropathy related to homozygous variants in IMMT gene. Clinical genetics 9 34842280
2024 Genetic ablation of Immt induces a lethal disruption of the MICOS complex. Life science alliance 8 38467404
2020 Effects of Aging on Expression of Mic60 and OPA1 and Mitochondrial Morphology in Myocardium of Tibetan Sheep. Animals : an open access journal from MDPI 8 33233488
2016 p53-dependent and -independent mechanisms are involved in (E)-1-(2-hydroxyphenyl)-3-(2-methoxynaphthalen-1-yl)prop-2-en-1-one (HMP)-induced apoptosis in HCT116 colon cancer cells. Biochemical and biophysical research communications 8 27641669
2014 The role of rpoS, hmp, and ssrAB in Salmonella enterica Gallinarum and evaluation of a triple-deletion mutant as a live vaccine candidate in Lohmann layer chickens. Journal of veterinary science 8 25549217
2011 Tomato LeTHIC is an Fe-requiring HMP-P synthase involved in thiamine synthesis and regulated by multiple factors. Plant & cell physiology 8 21511719
1996 Sp1 is critical for basal and E2-transactivated transcription from the bovine papillomavirus type 1 P89 promoter. The Journal of general virology 8 8627222
2024 The MICOS Complex Subunit Mic60 is Hijacked by Intracellular Bacteria to Manipulate Mitochondrial Dynamics and Promote Bacterial Pathogenicity. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 7 39431455
2018 Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28. Bioscience, biotechnology, and biochemistry 7 29985105
1987 HMP-shunt and cholesterol metabolism in experimental models involving normal and preneoplastic liver growth. Toxicologic pathology 7 3554466
2024 Suppressing mitochondrial inner membrane protein (IMMT) inhibits the proliferation of breast cancer cells through mitochondrial remodeling and metabolic regulation. Scientific reports 6 38834715
2024 Single-molecule force spectroscopy reveals intra- and intermolecular interactions of Caenorhabditis elegans HMP-1 during mechanotransduction. Proceedings of the National Academy of Sciences of the United States of America 6 39236238
2023 SRGP-1/srGAP and AFD-1/afadin stabilize HMP-1/⍺-catenin at rosettes to seal internalization sites following gastrulation in C. elegans. PLoS genetics 6 36867663
2021 HMP-S7 Is a Novel Anti-Leukemic Peptide Discovered from Human Milk. Biomedicines 6 34440185
2011 Nitrosative stress causes amino acid auxotrophy in hmp mutant Salmonella Typhimurium. Microbiology and immunology 6 21752086
2009 Expression of HMP/AN2, a melanoma associated antigen, in murine cerebral gliomas: potential for radioimmunotargeting. Journal of neuro-oncology 6 19152070
2024 Whole cell affinity for 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in the marine bacterium Candidatus Pelagibacter st. HTCC7211 explains marine dissolved HMP concentrations. Environmental microbiology reports 5 39367564
2023 Characterization of the enzyme kinetics of EMP and HMP pathway in Corynebacterium glutamicum: reference for modeling metabolic networks. Frontiers in bioengineering and biotechnology 5 38090711
2024 Electroacupuncture pretreatment maintains mitochondrial quality control via HO-1/MIC60 signaling pathway to alleviate endotoxin-induced acute lung injury. Biochimica et biophysica acta. Molecular basis of disease 4 39209235
2000 Improving the performance of an acid-labile 4-hydroxymethyl phenoxyacetic acid (HMP) linker on resin and SynPhase grafted solid-supports. Journal of peptide science : an official publication of the European Peptide Society 4 11071267
1994 Inhibition of two HMP shunt pathway enzymes by fatty acids and their CoA esters in developing human brain: role of fatty acid binding protein. Indian journal of biochemistry & biophysics 4 7875715
1988 Comparative effects of insulin and refeeding on DNA synthesis, HMP shunt and cholesterogenesis in diabetic and fasted rats. Pathology 4 3287306
2025 Ancestral sequence reconstruction of the Mic60 Mitofilin domain reveals residues supporting respiration in yeast. Protein science : a publication of the Protein Society 3 40545685
2023 Carnosic acid attenuated cytochrome c release through the mitochondrial structural protein Mic60 by PINK1 in SH-SY5Y cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 3 36708866
2017 [Correlation between Mic60 haploid insufficiency and cardiac aging in mouse]. Zhonghua bing li xue za zhi = Chinese journal of pathology 3 28591988
2016 Purification, crystallization and initial crystallographic analysis of the α-catenin homologue HMP-1 from Caenorhabditis elegans. Acta crystallographica. Section F, Structural biology communications 3 26919528
2014 Molecular, Physiological and Phenotypic Characterization of Paracoccus denitrificans ATCC 19367 Mutant Strain P-87 Producing Improved Coenzyme Q10. Indian journal of microbiology 3 25805905
2013 The influence of CsgD on the expression of genes of folate metabolism and hmp in Escherichia coli K-12. Archives of microbiology 3 23824318
2020 Functional characterization of the HMP-P synthase of Legionella pneumophila (Lpg1565). Molecular microbiology 2 33034117

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