Affinage

SAMM50

Sorting and assembly machinery component 50 homolog · UniProt Q9Y512

Length
469 aa
Mass
52.0 kDa
Annotated
2026-06-10
78 papers in source corpus 20 papers cited in narrative 18 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SAMM50/Sam50 is the essential beta-barrel core of the mitochondrial outer-membrane sorting and assembly machinery (SAM) complex, where it drives the membrane insertion of beta-barrel substrates including Tom40 and porin/VDAC, with matrix protein import remaining unaffected by its loss (PMID:14570913, PMID:14699090, PMID:17510655). Its barrel architecture folds through parallel pathways nucleated at the N-terminus, and its channel gating—rather than the POTRA domain—is the functionally critical feature for SAM-assisted assembly (PMID:41927578). Beyond substrate insertion, Sam50 physically bridges the outer and inner membranes by forming a Sam50-Mic19-Mic60 axis that joins the SAM and MICOS complexes into the larger MIB supercomplex, an interaction required for cristae junction formation, cristae integrity, and the assembly of respiratory chain complexes containing mitochondrially encoded subunits (PMID:22252321, PMID:31097788). Sam50 cooperates with Mic60 to bind cardiolipin and, together with MICOS and ATAD3, maintains mtDNA stability; its loss causes cardiolipin externalization, Bax recruitment, mtDNA release, and cGAS-STING-driven inflammation in liver (PMID:35313046). Sam50 additionally functions as a mitophagy receptor through a canonical LIR motif that engages ATG8-family proteins and p62/SQSTM1 to mediate piecemeal degradation of SAM and MICOS components (PMID:34037656, PMID:34275433), and it interacts with Drp1 to influence mitochondrial fission and inheritance (PMID:27059175). Its beta-barrel channel provides a TOM-independent route for cytotoxic granzymes to enter mitochondria during cell death (PMID:28338658), and proper mitochondrial targeting of Sam50 requires N-myristoylation of its N-terminus (PMID:30427857). A naturally occurring D110G variant impairs mitochondrial architecture and provokes metabolic dysfunction in vivo, linking SAMM50 to mitochondrial integrity in disease contexts [PMID:bio_10.1101_2025.06.04.657815].

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 2003 High

    Established that Sam50 is the essential machinery component for inserting beta-barrel proteins into the mitochondrial outer membrane, defining a dedicated assembly pathway distinct from matrix protein import.

    Evidence Yeast conditional omp85 mutants with biochemical fractionation and import assays

    PMID:14570913 PMID:14699090

    Open questions at the time
    • Did not resolve the structural basis of substrate recognition
    • Human relevance not yet tested
  2. 2007 Medium

    Extended the beta-barrel assembly role to human cells and placed Sam50 in a biogenesis pathway with TOM and metaxins, while revealing it also co-purifies with inner-membrane MICOS proteins, hinting at a trans-membrane bridging role.

    Evidence RNAi knockdown with native PAGE and co-IP in human cells; mitofilin immunocapture with mass spectrometry

    PMID:17510655 PMID:17624330

    Open questions at the time
    • Whether MICOS co-purification reflects direct binding was unresolved
    • Functional consequence of the large assembly not dissected
  3. 2012 High

    Showed that Sam50-anchored OMM-IMM contacts (the MIB complex) are required for cristae integrity and respiratory complex assembly, connecting outer-membrane machinery to inner-membrane organization.

    Evidence RNAi knockdown, co-IP, blue native PAGE, electron microscopy

    PMID:22252321

    Open questions at the time
    • Did not identify which direct interactions hold the bridge together
    • Mechanism linking contacts to respiratory assembly unclear
  4. 2015 Medium

    Demonstrated direct binding of Mitofilin and CHCHD6 to Sam50 and showed the MICOS-Sam50 interaction controls cristae morphology and membrane potential.

    Evidence TALEN knockout, immunoprecipitation, transmission EM, membrane potential measurement

    PMID:26530328

    Open questions at the time
    • Single-lab interaction mapping
    • Stoichiometry of the bridging complex not defined
  5. 2016 Medium

    Identified Sam50 as a physical Drp1 partner that influences fission machinery levels and mitochondrial inheritance, broadening its role into fission dynamics.

    Evidence Reciprocal co-IP and GST-pulldown, confocal microscopy, RNAi and overexpression

    PMID:27059175

    Open questions at the time
    • Mechanism by which Sam50 regulates Drp1 recruitment unknown
    • Single lab
  6. 2017 Medium

    Resolved the spatial organization of Sam50, showing punctate, non-uniform distribution that partially overlaps MICOS domains at crista junctions, and demonstrated its barrel mediates TOM-independent granzyme entry into mitochondria.

    Evidence miniSOG/APEX2 electron tomography; RNAi with import and ROS/cell-death assays plus granzyme B mutagenesis

    PMID:28338658 PMID:28808085

    Open questions at the time
    • How granzyme cargo is selected by the channel unresolved
    • Functional consequence of spatial domains not fully dissected
  7. 2018 Medium

    Established N-myristoylation as a requirement for Sam50 mitochondrial targeting and for MIC19-Sam50 interaction.

    Evidence Metabolic labeling, G2A mutagenesis, immunofluorescence, subcellular fractionation, co-IP

    PMID:30427857

    Open questions at the time
    • Enzyme catalyzing the modification not identified
    • Single lab
  8. 2019 High

    Defined the Sam50-Mic19-Mic60 axis as the molecular bridge uniting SAM and MICOS into the MIB supercomplex, with OMA1 cleavage of Mic19 acting as a regulatory switch for bridge integrity.

    Evidence Co-IP, super-resolution microscopy, OMA1 protease cleavage assays, ATP measurement

    PMID:31097788

    Open questions at the time
    • Structural detail of the axis lacking
    • Trigger for OMA1-mediated regulation in vivo unclear
  9. 2021 High

    Identified Sam50 as a LIR-motif mitophagy receptor mediating piecemeal degradation of SAM/MICOS components, and connected it to PINK1/Parkin-dependent mitophagy and lipid metabolism.

    Evidence Co-IP with LIR mutation and mitophagy flux assays; PINK1 co-IP with genetic epistasis; fatty acid oxidation and lipid accumulation assays

    PMID:33728819 PMID:34037656 PMID:34275433 PMID:34631840

    Open questions at the time
    • How piecemeal cargo selection is restricted to SAM/MICOS unclear
    • Lipid metabolism link is low-confidence and phenotypic only
  10. 2022 High

    Established the Sam50-MICOS-ATAD3-mtDNA axis and cardiolipin binding as guardians of mtDNA stability and membrane integrity, with loss driving Bax recruitment, mtDNA release, and cGAS-STING inflammation.

    Evidence Liver-specific Sam50 knockout mice, cardiolipin binding assays, acetaminophen model, cGAS-STING measurement, EM

    PMID:35313046

    Open questions at the time
    • Direct lipid-binding residues not mapped
    • Whether cardiolipin externalization is cause or consequence not fully resolved
  11. 2024 Medium

    Identified Shmt2 as a downstream Sam50 effector that limits Bax translocation and caspase-3 activation, extending Sam50's cardioprotective signaling.

    Evidence Co-IP/MS, overexpression and knockdown, Bax localization and caspase-3 assays, cardiac MI model

    PMID:38723737

    Open questions at the time
    • Direct vs indirect Sam50-Shmt2 interaction not confirmed
    • Single lab
  12. 2026 High

    Provided the biophysical folding and gating logic of the Sam50 barrel, showing N-terminal folding nucleation, a dispensable POTRA domain, and gating-linked stability hotspots that govern SAM-assisted assembly.

    Evidence Single-molecule electrophysiology, in vivo function assays, 165-variant alanine scanning, stability measurements

    PMID:41927578

    Open questions at the time
    • High-resolution structure of the assembled SAM with substrate not provided
    • Link between gating and substrate insertion mechanism incomplete
  13. 2025 Medium

    Linked a natural SAMM50 D110G variant to impaired mitochondrial architecture and metabolic disease phenotypes in vivo.

    Evidence CRISPR/Cas9 D110G knock-in mice, high-fat diet, mitochondrial function and metabolic phenotyping (preprint)

    PMID:bio_10.1101_2025.06.04.657815

    Open questions at the time
    • Preprint not yet peer-reviewed
    • Molecular mechanism of how D110G perturbs barrel function not defined

Open questions

Synthesis pass · forward-looking unresolved questions
  • How Sam50's multiple roles—barrel assembly, MIB bridging, mitophagy receptor, fission regulation, and cardiolipin/mtDNA maintenance—are coordinated or switched within a single protein remains unresolved.
  • No integrated structural model of Sam50 in distinct functional states
  • Regulatory logic partitioning its functions is unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0005215 transporter activity 2 GO:0060090 molecular adaptor activity 2 GO:0008289 lipid binding 1
Pathway
R-HSA-1852241 Organelle biogenesis and maintenance 2 R-HSA-392499 Metabolism of proteins 2 R-HSA-5357801 Programmed Cell Death 2 R-HSA-9609507 Protein localization 2 R-HSA-9612973 Autophagy 2
Partners
ATAD3ACHCHD6DNM1LMIC19MIC60MTX1MTX2SHMT2
Complex memberships
MIB supercomplexMICOS complexSAM complex

Evidence

Reading pass · 18 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 Sam50 (Omp85 family) is an essential subunit of the sorting and assembly machinery (SAM) complex in the mitochondrial outer membrane, required for the assembly of beta-barrel proteins Tom40 and porin (VDAC) into the outer membrane; yeast conditional omp85 mutants showed defective insertion of VDAC and TOM complex components, while import of matrix proteins was unaffected. Yeast conditional mutants, biochemical fractionation, import assays The Journal of biological chemistry High 14570913 14699090
2007 Sam50 depletion by RNAi in human cells causes strong defects in VDAC assembly and reduced steady-state VDAC levels; Sam50-depleted mitochondria also show reduced levels of metaxin 1 and 2, implying a connection between Sam50 and metaxins, though they appear to reside in distinct complexes. The pathway of VDAC biogenesis involves TOM complex, Sam50, and metaxins and is evolutionarily conserved. RNAi knockdown, import assays, native gel electrophoresis, co-immunoprecipitation EMBO reports High 17510655
2007 SAM50 co-immunoprecipitates with mitofilin (inner membrane), metaxins 1 and 2, CHCHD3, CHCHD6, and DnaJC11 as part of a large mitochondrial complex, indicating that Sam50 participates in a multi-protein assembly spanning the outer and inner mitochondrial membranes. Immunocapture with monoclonal antibody against mitofilin followed by mass spectrometry identification of co-purifying proteins FEBS letters Medium 17624330
2012 Sam50 is part of a large mitochondrial intermembrane space bridging (MIB) complex together with IMM proteins mitofilin and CHCHD3; disruption of MIB complex (via Sam50 depletion) causes loss of cristae integrity and deficiency in assembly of respiratory chain complexes containing mitochondrially encoded subunits, linking OMM-IMM contacts to respiratory complex biogenesis. RNAi knockdown, co-immunoprecipitation, blue native PAGE, electron microscopy, BN-PAGE respiratory complex assembly assays Molecular and cellular biology High 22252321
2015 Mitofilin and CHCHD6 directly interact with Sam50; immunoprecipitation experiments identified a complex containing Mitofilin, Sam50, CHCHD3, and CHCHD6. Knockout of Mitofilin (but not CHCHD6) disrupted binding partners controlling cristae morphology. Direct binding of Mitofilin and CHCHD6 to Sam50 was demonstrated, and MICOS-Sam50 interaction is required for cristae organization and mitochondrial membrane potential. TALEN-mediated knockdown/knockout, immunoprecipitation, transmission electron microscopy, mitochondrial membrane potential measurement Scientific reports Medium 26530328
2017 Granzyme B (and granzyme A and caspase-3) enter mitochondria through a pathway that requires Sam50 (the central SAM subunit) for outer membrane transit, independently of the TOM complex. Depletion of Sam50 reduces granzyme-mediated ROS production and cell death; mutation of GB residues K243/R244 also reduces mitochondrial entry. RNAi knockdown of Sam50, in vitro import assays, cell death/ROS measurement, site-directed mutagenesis of granzyme B Cell death and differentiation Medium 28338658
2018 SAMM50 is N-myristoylated at its N-terminus, and this lipid modification is required for proper mitochondrial targeting of SAMM50; the non-myristoylated G2A mutant fails to localize to mitochondria. N-myristoylation of MIC19 is also required for its interaction with SAMM50. In vitro and in vivo metabolic labeling, immunofluorescence, subcellular fractionation, immunoprecipitation with G2A mutant PloS one Medium 30427857
2019 Sam50 directly interacts with Mic19 (a MICOS component) and Mic60 to form the Sam50-Mic19-Mic60 axis, which connects the SAM and MICOS complexes into the MIB supercomplex. This axis mediates OMM-IMM contacts and is required for normal cristae junction formation and cristae distribution. OMA1-mediated cleavage of Mic19 disrupts this axis and leads to MIB disassembly. Sam50 displays punctate distribution at the OMM and acts as an anchoring point for cristae junction formation. Co-immunoprecipitation, super-resolution microscopy, OMA1 protease cleavage assays, MICOS/SAM complex disruption experiments, ATP measurement Cell death and differentiation High 31097788
2021 SAMM50 acts as a mitophagy receptor by directly interacting with ATG8 family proteins via a canonical LIR (LC3-interacting region) motif and with p62/SQSTM1, mediating basal piecemeal mitophagy of SAM and MICOS complex components. Upon metabolic switch to oxidative phosphorylation, SAMM50 cooperates with p62 to mediate efficient mitophagy of these components. Co-immunoprecipitation, LIR motif mutation, autophagy flux assays, mitophagy reporter assays The Journal of cell biology High 34037656 34275433
2022 Sam50 interacts with MICOS complex and ATAD3 to form the Sam50-MICOS-ATAD3-mtDNA axis, maintaining mtDNA stability. Sam50 cooperates with Mic60 to bind cardiolipin, maintaining mitochondrial membrane integrity. Sam50 depletion causes cardiolipin externalization, triggering Bax mitochondrial recruitment, mtDNA aggregation and release, and subsequent cGAS-STING pathway activation and liver inflammation. Co-immunoprecipitation, cardiolipin binding assays, liver-specific Sam50 knockout mice, ACETAMINOPHEN model, cGAS-STING pathway activation measurement, electron microscopy Hepatology (Baltimore, Md.) High 35313046
2016 SAMM50 overexpression causes Drp1-dependent mitochondrial fragmentation in HeLa cells, reversible by co-expression with MFN2. SAMM50 interacts physically with Drp1 both in vivo (co-IP) and in vitro (pulldown). SAMM50 knockdown leads to decreased mitochondrial Drp1 levels, decreased OPA1, and a swollen mitochondrial phenotype; mitochondrial inheritance is impaired in SAMM50-silenced cells. Co-immunoprecipitation, GST-pulldown in vitro, confocal microscopy, RNAi knockdown, overexpression FEBS letters Medium 27059175
2017 Nanoscale electron tomography mapping of tagged Sam50, Mic19, and Mic60 in mitochondria revealed that Sam50 is not uniformly distributed in the outer mitochondrial membrane and incompletely overlaps with Mic19- and Mic60-positive domains, particularly at crista junctions, suggesting spatially organized interactions. miniSOG and APEX2 genetic tagging, electron tomography Journal of cell science Medium 28808085
2021 SAMM50 deficiency in vitro results in increased lipid accumulation due to decreased fatty acid oxidation; overexpression of SAMM50 enhances fatty acid oxidation and mitigates intracellular lipid accumulation. In vitro cell culture, SAMM50 overexpression and knockdown, fatty acid oxidation assays, lipid accumulation staining FEBS open bio Low 33728819
2021 Samm50 interacts with Pink1 and stimulates accumulation of Parkin on mitochondria to initiate mitophagy; Samm50 overexpression reduces mitophagy and promotes cardiac hypertrophy, while Samm50 knockdown increases mitophagy and protects against hypertrophy. The protective effect of Samm50 deficiency against hypertrophy is abolished by inhibiting mitophagy through Vps34 inhibitor or Pink1 knockdown. Co-immunoprecipitation, lentiviral overexpression and knockdown, immunofluorescence, qPCR, pharmacological inhibition of mitophagy, genetic epistasis with Pink1 knockdown Frontiers in cardiovascular medicine Medium 34631840
2024 Sam50 interacts with serine hydroxymethyltransferase 2 (Shmt2); this interaction was identified by co-immunoprecipitation/mass spectrometry. Shmt2 acts downstream of Sam50 to hinder Bax translocation from cytoplasm to mitochondria and subsequent caspase-3 activation. Inhibition of Shmt2 abolished the cardioprotective effect of Sam50 overexpression. Co-immunoprecipitation/mass spectrometry, overexpression and knockdown, Bax localization assays, caspase-3 activity measurement, cardiac MI model in vivo Cellular signalling Medium 38723737
2011 Neisserial Omp85, but not other bacterial beta-barrel proteins, can be selectively imported and integrated into the outer mitochondrial membrane of human cells; this occurs via the TOM and SAM complexes. Omp85 alone enables integration of other bacterial beta-barrel proteins in human mitochondria but cannot substitute for the function of Sam50, demonstrating that Sam50 has species-specific functions not replaceable by its bacterial homolog. In vitro import assays in isolated human mitochondria, RNAi depletion of SAM/TOM components, native PAGE The Journal of biological chemistry Medium 21652692
2026 Sam50 folds through parallel pathways with at least two transition states; the folding nucleus is at the N-terminus while the C-terminal region is kinetically trapped. The POTRA domain is dispensable for Sam50 channel function. Destabilizing hotspot residues in Sam50 are linked to its gating function, and 165 Ala substitution scan correlated per-residue stability with SAM-assisted assembly. Single-molecule electrophysiology, in vivo function assays, alanine scanning mutagenesis (165 substitutions), stability measurements Nature communications High 41927578
2025 The SAMM50 rs3761472 SNP (encoding a D110G substitution) impairs mitochondrial integrity by downregulating key regulators of mitochondrial architecture, dynamics, and quality control; Samm50-KI mice show reduced ATP production, elevated oxidative stress and inflammation, insulin resistance, glucose intolerance, and pronounced hepatic lipid accumulation on a high-fat diet. CRISPR/Cas9 knock-in mice with D110G substitution, high-fat diet feeding, mitochondrial function assays, metabolic phenotyping bioRxivpreprint Medium bio_10.1101_2025.06.04.657815

Source papers

Stage 0 corpus · 78 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2003 The Omp85 family of proteins is essential for outer membrane biogenesis in mitochondria and bacteria. The Journal of cell biology 314 14699090
2006 Assembly factor Omp85 recognizes its outer membrane protein substrates by a species-specific C-terminal motif. PLoS biology 280 17090219
2003 An essential role of Sam50 in the protein sorting and assembly machinery of the mitochondrial outer membrane. The Journal of biological chemistry 273 14570913
2007 Structure of the membrane protein FhaC: a member of the Omp85-TpsB transporter superfamily. Science (New York, N.Y.) 213 17702945
2005 Molecular architecture and function of the Omp85 family of proteins. Molecular microbiology 189 16313611
2012 Sam50 functions in mitochondrial intermembrane space bridging and biogenesis of respiratory complexes. Molecular and cellular biology 188 22252321
2007 The mitochondrial inner membrane protein mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11. FEBS letters 180 17624330
2013 Genome-wide scan revealed that polymorphisms in the PNPLA3, SAMM50, and PARVB genes are associated with development and progression of nonalcoholic fatty liver disease in Japan. Human genetics 165 23535911
2005 YaeT (Omp85) affects the assembly of lipid-dependent and lipid-independent outer membrane proteins of Escherichia coli. Molecular microbiology 151 16102012
2009 The Bam (Omp85) complex is involved in secretion of the autotransporter haemoglobin protease. Microbiology (Reading, England) 112 19815580
2015 Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure. Scientific reports 108 26530328
2004 Omp85, an evolutionarily conserved bacterial protein involved in outer-membrane-protein assembly. Research in microbiology 106 15143770
2007 Functioning of outer membrane protein assembly factor Omp85 requires a single POTRA domain. EMBO reports 98 18007659
2022 Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury. Hepatology (Baltimore, Md.) 97 35313046
2007 Conserved roles of Sam50 and metaxins in VDAC biogenesis. EMBO reports 96 17510655
2019 Sam50-Mic19-Mic60 axis determines mitochondrial cristae architecture by mediating mitochondrial outer and inner membrane contact. Cell death and differentiation 92 31097788
2003 The Omp85 protein of Neisseria meningitidis is required for lipid export to the outer membrane. The EMBO journal 90 12682011
2014 A comprehensive analysis of the Omp85/TpsB protein superfamily structural diversity, taxonomic occurrence, and evolution. Frontiers in microbiology 85 25101071
2015 Targeted-bisulfite sequence analysis of the methylation of CpG islands in genes encoding PNPLA3, SAMM50, and PARVB of patients with non-alcoholic fatty liver disease. Journal of hepatology 70 25776890
2006 Functional and phylogenetic properties of the pore-forming beta-barrel transporters of the Omp85 family. The Journal of biological chemistry 70 17088246
2009 Role of periplasmic chaperones and BamA (YaeT/Omp85) in folding and secretion of intimin from enteropathogenic Escherichia coli strains. Journal of bacteriology 68 19525348
2021 SAMM50 acts with p62 in piecemeal basal- and OXPHOS-induced mitophagy of SAM and MICOS components. The Journal of cell biology 62 34037656
2010 Omp85 from the thermophilic cyanobacterium Thermosynechococcus elongatus differs from proteobacterial Omp85 in structure and domain composition. The Journal of biological chemistry 60 20351097
2009 Filling the gap, evolutionarily conserved Omp85 in plastids of chromalveolates. The Journal of biological chemistry 60 20042599
2009 First structural insights into the TpsB/Omp85 superfamily. Biological chemistry 58 19558323
2018 Genetic Polymorphisms of PNPLA3 and SAMM50 Are Associated with Nonalcoholic Fatty Liver Disease in a Korean Population. Gut and liver 54 29271184
2004 Evidence for conservation of architecture and physical properties of Omp85-like proteins throughout evolution. Proceedings of the National Academy of Sciences of the United States of America 49 15381771
2010 Conserved properties of polypeptide transport-associated (POTRA) domains derived from cyanobacterial Omp85. The Journal of biological chemistry 48 20348103
2010 Immunization with the recombinant Burkholderia pseudomallei outer membrane protein Omp85 induces protective immunity in mice. Vaccine 48 20546831
2015 Assembly of the secretion pores GspD, Wza and CsgG into bacterial outer membranes does not require the Omp85 proteins BamA or TamA. Molecular microbiology 47 25976323
2014 Translocation path of a substrate protein through its Omp85 transporter. Nature communications 41 25327833
2022 Function of the Omp85 Superfamily of Outer Membrane Protein Assembly Factors and Polypeptide Transporters. Annual review of microbiology 40 35650668
2017 Granzyme B enters the mitochondria in a Sam50-, Tim22- and mtHsp70-dependent manner to induce apoptosis. Cell death and differentiation 40 28338658
1998 Omp85 proteins of Neisseria gonorrhoeae and Neisseria meningitidis are similar to Haemophilus influenzae D-15-Ag and Pasteurella multocida Oma87. Microbial pathogenesis 40 9705245
2007 Glycosylation of the OMP85 homolog of Porphyromonas gingivalis and its involvement in biofilm formation. Biochemical and biophysical research communications 37 18029265
2015 Conserved Omp85 lid-lock structure and substrate recognition in FhaC. Nature communications 34 26058369
2010 Functional importance of a conserved sequence motif in FhaC, a prototypic member of the TpsB/Omp85 superfamily. The FEBS journal 33 20955520
2018 Genetic variants in COL13A1, ADIPOQ and SAMM50, in addition to the PNPLA3 gene, confer susceptibility to elevated transaminase levels in an admixed Mexican population. Experimental and molecular pathology 31 29307798
2011 Omp85 in eukaryotic systems: one protein family with distinct functions. Biological chemistry 30 21194359
2011 Neisserial Omp85 protein is selectively recognized and assembled into functional complexes in the outer membrane of human mitochondria. The Journal of biological chemistry 28 21652692
2021 The role of SAMM50 in non-alcoholic fatty liver disease: from genetics to mechanisms. FEBS open bio 27 33728819
2014 Structure and function of POTRA domains of Omp85/TPS superfamily. International review of cell and molecular biology 27 24411168
2008 Omp85(Tt) from Thermus thermophilus HB27: an ancestral type of the Omp85 protein family. Journal of bacteriology 27 18456816
2018 Identification and characterization of protein N-myristoylation occurring on four human mitochondrial proteins, SAMM50, TOMM40, MIC19, and MIC25. PloS one 26 30427857
2017 Sub-mitochondrial localization of the genetic-tagged mitochondrial intermembrane space-bridging components Mic19, Mic60 and Sam50. Journal of cell science 26 28808085
2007 The invariant phenylalanine of precursor proteins discloses the importance of Omp85 for protein translocation into cyanelles. BMC evolutionary biology 24 18045484
2024 Lycopene Protects against Atrazine-Induced Kidney STING-Dependent PANoptosis through Stabilizing mtDNA via Interaction with Sam50/PHB1. Journal of agricultural and food chemistry 23 38820047
2015 Genetic Variants in the SAMM50 Gene Create Susceptibility to Nonalcoholic Fatty Liver Disease in a Chinese Han Population. Hepatitis monthly 21 26587038
2020 SAM50, a side door to the mitochondria: The case of cytotoxic proteases. Pharmacological research 20 32919042
2007 Characterisation of YtfM, a second member of the Omp85 family in Escherichia coli. Biological chemistry 20 17214547
2016 SAMM50 Affects Mitochondrial Morphology through the Association of Drp1 in Mammalian Cells. FEBS letters 19 27059175
2005 Evidence of positive Darwinian selection in Omp85, a highly conserved bacterial outer membrane protein essential for cell viability. Journal of molecular evolution 16 15785855
2016 Relative Orientation of POTRA Domains from Cyanobacterial Omp85 Studied by Pulsed EPR Spectroscopy. Biophysical journal 15 27224485
2015 Dynamic interplay of membrane-proximal POTRA domain and conserved loop L6 in Omp85 transporter FhaC. Molecular microbiology 13 26192332
2013 Omp85 genosensor for detection of human brain bacterial meningitis. Biotechnology letters 12 23471585
2021 Samm50 Promotes Hypertrophy by Regulating Pink1-Dependent Mitophagy Signaling in Neonatal Cardiomyocytes. Frontiers in cardiovascular medicine 10 34631840
2021 Interaction of SAMM50-rs738491, PARVB-rs5764455 and PNPLA3-rs738409 Increases Susceptibility to Nonalcoholic Steatohepatitis. Journal of clinical and translational hepatology 10 35528982
2016 Depletion of SAM50 Specifically Targets BCR-ABL-Expressing Leukemic Stem and Progenitor Cells by Interfering with Mitochondrial Functions. Stem cells and development 10 26855047
2022 Sam50 exerts neuroprotection by maintaining the mitochondrial structure during experimental cerebral ischemia/reperfusion injury in rats. CNS neuroscience & therapeutics 9 36074556
2021 SAMM50 is a receptor for basal piecemeal mitophagy and acts with SQSTM1/p62 in OXPHOS-induced mitophagy. Autophagy 9 34275433
2022 SAMM50 Regulates Thermogenesis of Beige Adipocytes Differentiated from Human Adipose-Derived Stem Cells by Balancing Mitochondrial Dynamics. International journal of molecular sciences 8 35743205
2013 Quick diagnosis of human brain meningitis using omp85 gene amplicon as a genetic marker. Indian journal of microbiology 7 24426115
2024 Ablation of Sam50 is associated with fragmentation and alterations in metabolism in murine and human myotubes. Journal of cellular physiology 6 38770789
2024 PNPLA3 and SAMM50 variants are associated with lean nonalcoholic fatty liver disease in Asian population. Annals of hepatology 6 39638042
2021 TtOmp85, a single Omp85 member protein functions as a β-barrel protein insertase and an autotransporter translocase without any accessory proteins. Biochemical and biophysical research communications 5 33743350
2019 Tic22 from Anabaena sp. PCC 7120 with holdase function involved in outer membrane protein biogenesis shuttles between plasma membrane and Omp85. Molecular microbiology 5 30742726
2018 An investigation into the Omp85 protein BamK in hypervirulent Klebsiella pneumoniae, and its role in outer membrane biogenesis. Molecular microbiology 4 29873128
2025 Based on the Sam50-MICOS-ATAD3-mtDNA axis: Exploring oligomeric proanthocyanidins to alleviate molybdenum and cadmium co-induced liver inflammation in sheep. International journal of biological macromolecules 3 39954884
2024 Mitochondrial outer membrane protein Samm50 protects against hypoxia-induced cardiac injury by interacting with Shmt2. Cellular signalling 2 38723737
2023 SAMM50-rs2073082, -rs738491 and -rs3761472 Interactions Enhancement of Susceptibility to Non-Alcoholic Fatty Liver Disease. Biomedicines 2 37760857
2026 Linking multipoint folding and stability with functional regulation in the mitochondrial transmembrane β-barrel Sam50. Nature communications 1 41927578
2024 MICOS Complex Loss Governs Age-Associated Murine Mitochondrial Architecture and Metabolism in the Liver, While Sam50 Dictates Diet Changes. bioRxiv : the preprint server for biology 1 38979162
2023 Ablation of Sam50 is associated with fragmentation and alterations in metabolism in murine and human myotubes. bioRxiv : the preprint server for biology 1 37292887
2026 Borrelia BBJ25 is a plasmid-encoded Omp85-family protein associated with a potential export system. Bioscience reports 0 41649078
2026 Nutrient State, Aging, and Diet Modulate SAM50-Dependent Mitochondrial Remodeling and Systemic Metabolic Signatures. Research square 0 41756451
2026 The Omp85 family protein, TamA, exhibits characteristics of a suitable drug target against Pseudomonas aeruginosa. Microbiology (Reading, England) 0 42012866
2026 Riemerella anatipestifer OMP85, a BamA family outer membrane protein, enhances virulence through recruiting host complement regulator vitronectin to mediate complement evasion. Journal of immunology (Baltimore, Md. : 1950) 0 42019960
2024 Leptospira interrogans encodes a canonical BamA and three novel noNterm Omp85 outer membrane protein paralogs. Scientific reports 0 39198480

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