Affinage

HOXB4

Homeobox protein Hox-B4 · UniProt P17483

Length
251 aa
Mass
27.6 kDa
Annotated
2026-06-10
100 papers in source corpus 42 papers cited in narrative 42 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

HOXB4 is a homeodomain transcription factor that acts as a master positive regulator of hematopoietic stem cell (HSC) self-renewal and proliferation, expanding the most primitive transplantable HSC compartment without disrupting lineage differentiation (PMID:7622039, PMID:11955445). Its expansion activity requires direct DNA binding by the homeodomain but is independent of, and even enhanced by loss of, the HOX-PBX cooperative interaction (PMID:15226173); the protein is membrane-permeant, such that recombinant or stroma-secreted HOXB4 expands both murine and human HSCs without genetic modification (PMID:14578881, PMID:14578882). Crucially, HOXB4 does not override homeostatic ceilings on HSC pool size, restoring rather than exceeding normal HSC numbers in vivo (PMID:10515864), and loss-of-function studies show it is dispensable for HSC generation yet required for full proliferative capacity of stem/progenitor cells, acting redundantly with paralogous group-4 Hox genes (PMID:14962901, PMID:12748289, PMID:7628700). Mechanistically, HOXB4 directly binds and activates a network of hematopoietic master regulators—Runx1, Scl/Tal1, Gata2, Gfi1—and the downstream effector Hemgn, while acting context-dependently as a repressor of a subset of targets (PMID:21343615, PMID:20393131); it drives hemogenic endothelium formation upstream of Runx1 (PMID:29456178) and signals through a shared pathway with STAT3 (PMID:24446131). HOXB4 transcription is controlled at its promoter by USF1/USF2 acting through MAP kinase/p38 signaling downstream of thrombopoietin, with USF1 recruiting the hSET1A H3K4 methyltransferase complex, and by the trimeric NF-Y complex, which physically associates with USF1/2 (PMID:11085749, PMID:12855555, PMID:12791656, PMID:23754954). The protein is held under tight control: it has a short half-life imposed by CUL4-mediated ubiquitination of a conserved homeodomain degron (PMID:23520338), and it conversely assembles a Roc1-Ddb1-Cul4a E3 ligase complex to degrade Geminin and promote proliferation (PMID:21098278). HOXB4 limits its own oncogenic potential through a unique conserved N-terminal proline-rich domain and by repressing Prdm16, with deletion of the proline-rich domain or co-expression of Prdm16 converting HOXB4 into a leukemogenic driver (PMID:27827825, PMID:25082879, PMID:18357342). In non-hematopoietic and developmental contexts, HOXB4 expression is initiated in the hindbrain by somite-derived retinoic acid acting through a RARE in a prerhombomeric enhancer (PMID:9697850), and it functions as a context-dependent transcriptional regulator, directly repressing β-catenin to inhibit Wnt signaling in cervical cancer and activating DHDDS in ovarian cancer (PMID:33479226, PMID:32178630).

Mechanistic history

Synthesis pass · year-by-year structured walk · 19 steps
  1. 1995 High

    Established the foundational function of HOXB4 by asking whether a HOX gene could regulate HSC numbers, showing it selectively expands the primitive stem cell compartment.

    Evidence Retroviral overexpression in murine bone marrow with serial transplantation and limit-dilution repopulation assays

    PMID:7622039

    Open questions at the time
    • Direct transcriptional targets unidentified
    • Mechanism distinguishing self-renewal from differentiation unclear
  2. 1995 High

    Defined the physiological developmental role and revealed functional redundancy by asking what group-4 Hox genes are required for, showing dose-dependent vertebral transformations only in compound mutants.

    Evidence Targeted gene disruption generating double and triple Hox group-4 compound mutant mice with skeletal analysis

    PMID:7628700

    Open questions at the time
    • Does not address hematopoietic requirement
    • Molecular targets in skeletal patterning not defined
  3. 1998 High

    Addressed how HOXB4 is positioned in the embryo by identifying the upstream inducing signal, showing retinoic acid from somites sets the anterior expression boundary via a RARE.

    Evidence Chick/mouse tissue transposition, RAR inhibition, and enhancer-reporter transgenics with RARE mutagenesis

    PMID:9697850

    Open questions at the time
    • Hematopoietic regulation distinct from this neural enhancer
    • Downstream neural targets not defined
  4. 1998 High

    Tested which protein domains mediate HOXB4 activity in a transformation context, showing dependence on both the tetrapeptide HOX-PBX interaction domain and the homeodomain.

    Evidence Rat-1 fibroblast transformation assay with HOXB4 domain mutants and PBX1 modulation

    PMID:9692548

    Open questions at the time
    • Fibroblast transformation may not reflect HSC mechanism
    • PBX1 requirement later shown dispensable in HSCs
  5. 2002 High

    Asked whether HOXB4 acts ex vivo and on embryonic progenitors, demonstrating massive ex vivo HSC expansion and conferral of definitive engraftment potential on primitive progenitors.

    Evidence Retroviral overexpression in bone marrow, ES/yolk-sac progenitors, stromal co-culture, and competitive repopulation assays

    PMID:11955444 PMID:11955445

    Open questions at the time
    • Transcriptional program driving expansion unknown
    • Mechanism of definitive switch not resolved
  6. 2003 High

    Resolved whether HOXB4 protein itself suffices, showing membrane-permeant recombinant and stroma-secreted HOXB4 expand murine and human HSCs without gene transfer.

    Evidence TAT-HOXB4 protein transduction and stroma-secreted HOXB4 co-culture with murine and human HSCs, in vivo repopulation

    PMID:14578881 PMID:14578882

    Open questions at the time
    • Mechanism of passive membrane translocation not detailed
    • Magnitude lower than retroviral delivery
  7. 2003 Medium

    Connected HOXB4 transcription to extrinsic cytokine signaling by showing thrombopoietin induces it via p38 MAPK and USF-1.

    Evidence TPO stimulation, p38 inhibition, USF-1 activation assays, and tpo-/- mouse mRNA quantification

    PMID:12855555

    Open questions at the time
    • Single lab
    • Quantitative contribution of TPO to physiological HOXB4 levels modest (2-5 fold)
  8. 2000 High

    Identified the proximal transcriptional activators of HOXB4 by asking what binds its promoter E-box, showing USF-1/USF-2 activate via the MAP kinase pathway.

    Evidence Yeast one-hybrid screen, EMSA, promoter mutagenesis, and luciferase assays in K562/CD34+ cells

    PMID:11085749

    Open questions at the time
    • MITF binds the same site without activating, function unexplained
    • In vivo promoter occupancy not addressed
  9. 2003 High

    Expanded the promoter model by identifying NF-Y as a developmentally regulated activator that physically partners with USF1/2.

    Evidence EMSA, ChIP, dominant-negative NF-Y, and co-immunoprecipitation of NF-Y/USF1/2

    PMID:12791656

    Open questions at the time
    • Mechanism linking NF-Ya decline to differentiation incomplete
  10. 2003 High

    Determined the dominant domain requirement for HSC expansion in vivo, showing direct DNA binding is essential while HOX-PBX cooperativity is dispensable and limiting.

    Evidence Retroviral DNA-binding-incompetent and PBX-interaction-deficient HOXB4 mutants with PBX1 knockdown and competitive repopulation

    PMID:12705858 PMID:15226173

    Open questions at the time
    • Non-cell-autonomous mechanism limiting expansion unidentified
    • Direct in vivo target genes not yet mapped
  11. 1999 Medium

    Established a non-self-renewal transcriptional function by asking how vitamin D3 suppresses c-myc, identifying HOXB4 as the MIE1-binding factor blocking c-myc elongation.

    Evidence EMSA protein identification, c-myc reporter assays, PKCβ inhibition, and antisense HOXB4 knockdown in HL-60

    PMID:10085075 PMID:11250656

    Open questions at the time
    • Single cell-line system
    • Relationship to HSC role unclear
  12. 2009 High

    Revealed translational control of HOXB4 by showing cytoplasmic Prep1-4EHP represses Hoxb4 mRNA via its 3'UTR.

    Evidence Co-IP, pulldown, 4EHP-motif mutagenesis, in vitro translation with Hoxb4-3'UTR reporter, and Prep1 hypomorph mice

    PMID:19365557

    Open questions at the time
    • Demonstrated in oocytes; relevance to hematopoiesis untested
    • Single study
  13. 2010 High

    Began defining the HOXB4 downstream program by identifying Hemgn as a direct target that partially mediates myeloid expansion.

    Evidence Inducible HOXB4-ER, expression microarray, ChIP at Hemgn promoter, and Hemgn gain/loss-of-function colony assays

    PMID:20393131

    Open questions at the time
    • Hemgn only partially recapitulates HOXB4 effect
    • Other targets needed for full activity
  14. 2010 Medium

    Uncovered a non-transcriptional role for HOXB4 as part of an E3 ligase, showing it assembles RDCOXB4 (Roc1-Ddb1-Cul4a) to degrade Geminin and promote proliferation.

    Evidence Co-IP of the Roc1-Ddb1-Cul4a complex, ubiquitination assays, and Geminin gain/loss-of-function with competitive repopulation

    PMID:21098278

    Open questions at the time
    • Single lab
    • Structural basis of complex assembly unknown
    • Relation to transcriptional activity unclear
  15. 2011 Medium

    Mapped the genome-wide direct target network, identifying Runx1, Scl/Tal1, Gata2 and Gfi1 as direct targets and showing context-dependent activation versus repression.

    Evidence ChIP-seq and genome-wide expression profiling in ES-derived HSPCs with inducible HoxB4

    PMID:21343615 PMID:22438249

    Open questions at the time
    • Per-target functional validation limited
    • Determinants of activation versus repression unresolved
  16. 2013 Medium

    Established post-translational and chromatin-level layers of regulation: CUL4 degrades HOXB4 via a conserved homeodomain degron, while USF1 recruits hSET1A to deposit activating H3K4me3.

    Evidence Co-IP with CUL4, degron mapping, degradation-resistant variants in human HSC transplants, and ChIP for H3K4me3/USF1/hSET1A in ESC differentiation

    PMID:23520338 PMID:23754954

    Open questions at the time
    • Degron degradation signal and E3 substrate-recognition details incomplete
    • Single labs
  17. 2014 Medium

    Defined how HOXB4 expansion is decoupled from leukemia, showing repression of Prdm16 prevents transformation and placing HoxB4 in a shared STAT3 self-renewal pathway.

    Evidence Expression profiling and sPrdm16 co-expression leukemia model; STAT3 co-transduction/inhibition with phospho-STAT3 and transcriptome overlap

    PMID:24446131 PMID:25082879

    Open questions at the time
    • Mechanism of STAT3 ligand-independent phosphorylation unknown
    • Direct versus indirect Prdm16 repression not fully resolved
  18. 2016 High

    Identified the structural basis restraining oncogenicity, showing a unique conserved N-terminal proline-rich domain limits stem cell amplification.

    Evidence Domain deletion and reciprocal HOXB4/HOXA9 domain-swap mutagenesis with in vivo leukemia monitoring

    PMID:18357342 PMID:27827825

    Open questions at the time
    • Molecular partners of the proline-rich domain unknown
    • Mechanism by which it restrains amplification undefined
  19. 2021 Medium

    Extended HOXB4 function to non-hematopoietic cancer contexts, showing direct transcriptional regulation of β-catenin, DHDDS, and additional targets.

    Evidence ChIP/reporter binding assays, gain/loss-of-function, rescue, and xenografts in cervical and ovarian cancer cells

    PMID:31768017 PMID:32178630 PMID:33479226

    Open questions at the time
    • Context determinants of repression versus activation unclear
    • Single labs per tumor type

Open questions

Synthesis pass · forward-looking unresolved questions
  • The integration of HOXB4's transcriptional, E3-ligase, and translational/post-translational regulatory layers into a unified model of how the HSC expansion ceiling is enforced remains unresolved.
  • The non-cell-autonomous mechanism capping HSC expansion is unidentified
  • How direct DNA targets, Geminin degradation, and STAT3 signaling converge mechanistically is unknown
  • Physiological relevance of Prep1-4EHP and miR-23a/IGF2BP1 mRNA regulation in HSCs untested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 6 GO:0003677 DNA binding 4 GO:0140096 catalytic activity, acting on a protein 1
Localization
GO:0005634 nucleus 3
Pathway
R-HSA-1266738 Developmental Biology 3 R-HSA-74160 Gene expression (Transcription) 3 R-HSA-392499 Metabolism of proteins 2
Partners
CUL4GEMININNFYAPBX1PREP1STAT3USF1USF2
Complex memberships
RDCOXB4 (Roc1-Ddb1-Cul4a E3 ligase)

Evidence

Reading pass · 42 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 Retroviral overexpression of HOXB4 in murine bone marrow cells selectively expands the most primitive hematopoietic stem cell (HSC) compartment, resulting in ~50-fold higher numbers of transplantable totipotent HSCs in primary and secondary recipients, without perturbing lineage differentiation or causing overt pathology. HOXB4 functions as a transcription factor regulating HSC self-renewal and proliferative potential. Retroviral gene transfer into murine bone marrow, serial transplantation, limit dilution assay for long-term competitive repopulating cells, in vitro colony assays Genes & development High 7622039
2002 HOXB4 expression in primitive yolk-sac or embryonic stem (ES) cell-derived progenitors, combined with culture on hematopoietic stroma, induces a switch to the definitive HSC phenotype capable of long-term multilineage engraftment in lethally irradiated adults, demonstrating HOXB4 confers definitive lymphoid-myeloid engraftment potential on primitive progenitors. Retroviral HOXB4 overexpression in ES-cell and yolk-sac progenitors, stromal co-culture, transplantation into lethally irradiated mice, multilineage reconstitution assay in primary and secondary recipients Cell High 11955444
2002 HOXB4 overexpression in murine bone marrow enables over 1000-fold higher HSC levels relative to controls and a 40-fold net HSC increase ex vivo, with HSCs retaining full lympho-myeloid repopulating potential, establishing HOXB4 as a potent positive regulator of HSC self-renewal ex vivo. Retroviral HOXB4 transduction of murine bone marrow, 10-14 day expansion culture, limit dilution competitive repopulating unit (CRU) assay, transplantation Cell High 11955445
1998 Cellular transformation and proliferation induced by HOXB4 (and HOXB3) in Rat-1 fibroblasts requires cooperation with PBX1; the transforming capacity depends on the conserved tetrapeptide (HOX-PBX interaction domain) and the homeodomain (DNA-binding domain). Modulating PBX1 levels directly modulates HOXB4-induced transformation. Rat-1 fibroblast transformation assay, overexpression and knockdown of PBX1, HOXB4 point mutants lacking tetrapeptide or homeodomain function Oncogene High 9692548
2003 Recombinant TAT-HOXB4 protein (carrying the HIV-TAT protein transduction domain) directly transduces into HSCs and expands murine HSCs ~4–6 fold ex vivo, comparable to retroviral HOXB4, demonstrating that HOXB4 protein itself (not just gene expression) is sufficient to drive HSC expansion and that the protein passively translocates across cell membranes. Recombinant protein production, protein transduction into murine bone marrow HSCs, competitive repopulation assay in transplanted mice Nature medicine High 14578881 14578882
2003 Human HSCs (LTC-ICs and NOD-SCID repopulating cells) can be expanded >20-fold (LTC-IC) and >2.5-fold (SRC) by HOXB4 protein delivered via stromal cells engineered to secrete it, confirming the membrane-translocating property of the HOXB4 homeoprotein and its ability to expand human HSCs without genetic modification. Stromal cells engineered to secrete HOXB4, co-culture with human HSCs, LTC-IC assay, NOD-SCID repopulation assay Nature medicine High 14578882
2003 HOXB4-induced HSC expansion in vivo is limited by the availability of PBX1: knockdown of PBX1 in HOXB4-overexpressing HSCs generates 'ultra-competitive' HSCs that are >20-fold more competitive than HOXB4-alone cells, yet still do not exceed normal HSC pool size in vivo, suggesting a non-cell-autonomous mechanism limits expansion. Retroviral overexpression of HOXB4 combined with PBX1 shRNA knockdown in murine bone marrow, competitive transplantation, multilineage reconstitution Immunity High 12705858
2004 HOXB4-induced HSC expansion requires direct DNA binding by HOXB4: a DNA-binding-incompetent HOXB4 mutant (HOXB4-A) fails to enhance HSC proliferation in vitro or expansion in vivo. Conversely, a mutant lacking HOX-PBX cooperative DNA binding (HOXB4-W→G) retains and even enhances in vitro proliferation and in vivo expansion, demonstrating that HOXB4 acts through direct DNA binding independent of HOX-PBX cooperative interaction. Retroviral overexpression of HOXB4 point mutants (DNA-binding-incompetent and PBX-interaction-deficient) in murine bone marrow, in vitro proliferation assay, in vivo competitive repopulation Blood High 15226173
2000 HOXB4 transcription in human hematopoietic cells is activated through an E-box binding site (HXRE-2) in its promoter by USF-1 and USF-2 transcription factors acting via the MAP kinase pathway; USF-2 was identified by yeast one-hybrid screen of bone marrow library. MITF binds the same site but does not activate the promoter. Promoter deletion/mutation analysis, yeast one-hybrid screen, EMSA, co-transfection luciferase assays in K562 and CD34+ cells, MAP kinase pathway inhibition The Journal of experimental medicine High 11085749
2003 The trimeric NF-Y complex binds the HxRE-1 site of the HOXB4 promoter, activates HOXB4 transcription in hematopoietic cells, and physically interacts with USF1/2 on the HOXB4 promoter; NF-Y occupancy on the HOXB4 promoter is reduced during granulocytic differentiation as NF-Ya levels decline, suggesting NF-Y is a developmentally regulated inducer of HOXB4. Promoter analysis, EMSA, ChIP assay, co-transfection with dominant-negative NF-Y subunits, co-immunoprecipitation of NF-Y/USF1/2 complex Blood High 12791656
1998 Hoxb4 rhombomeric expression in the vertebrate hindbrain is initiated by transient signaling from paraxial mesoderm (somites) through a retinoid pathway requiring retinoic acid receptor (RAR) function within the neural plate; a retinoic acid response element (RARE) within a prerhombomeric enhancer of Hoxb4 is essential for this neural response and interprets positional information for setting the anterior boundary of expression. Chick/mouse embryo tissue transposition, RAR inhibition in neural plate, enhancer-reporter transgenic mice, mutagenesis of RA response element Neuron High 9697850
1995 Compound mutants for paralogous group 4 Hox genes (hoxa-4, hoxb-4, hoxd-4) show more complete and dose-dependent homeotic transformations of cervical vertebrae than single mutants, demonstrating functional redundancy among paralogous genes and revealing a larger anteroposterior domain of requirement than single mutants indicate. Targeted gene disruption in mice (gene targeting), generation of double and triple Hox group 4 compound mutants, skeletal phenotype analysis Genes & development High 7628700
1999 HOXB4-overexpressing HSCs regenerate to fully reconstitute the HSC compartment post-transplantation (14-fold more HSCs than control transplants) but do not expand above normal HSC levels found in unmanipulated mice, indicating that HOXB4 overexpression does not override homeostatic mechanisms controlling HSC pool size. Murine bone marrow transplantation with HOXB4-retroviral transduced cells, HSC quantification by competitive repopulation over 12 months Blood High 10515864
2003 Hematopoietic expression of HOXB4 is regulated by thrombopoietin (TPO) via the p38 MAP kinase pathway, which induces upstream stimulating factor-1 (USF-1), which in turn activates HOXB4 transcription; TPO-null mice have 2–5-fold lower Hoxb4 expression in candidate HSCs. TPO stimulation of primitive hematopoietic cell lines, p38 MAPK pharmacological inhibition, comparison of tpo-/- and control mouse HSC Hoxb4 mRNA levels, USF-1 activation assays Blood Medium 12855555
2009 Cytoplasmic Prep1 interacts with 4EHP (eukaryotic translation initiation factor 4E homolog protein) in mouse oocytes and together they repress translation of Hoxb4 mRNA by Prep1 binding to the Hoxb4 3'UTR; a functional 4EHP-binding motif in Prep1 is required for this repression. Hoxb4 is overproduced in hypomorphic Prep1 oocytes, establishing Prep1-4EHP as a translational repressor of Hoxb4. Confocal microscopy/co-localization, co-immunoprecipitation, pulldown, site-directed mutagenesis of 4EHP-binding motif, in vitro translation assay with luciferase-Hoxb4-3'UTR reporter, RNA EMSA, immunohistochemistry in Prep1 hypomorphic mice PloS one High 19365557
2003 Multiple regulatory elements control Hoxb4 mesodermal expression: an intronic enhancer (region C) is sufficient for temporal activation and setting the anterior somitic boundary (somite 6/7), but the Hoxb4 promoter is required to maintain expression beyond E8.5; sequences in the 3'UTR (region B) are necessary specifically to maintain expression in somite 7 from E9.0 onward. Post-transcriptional regulation including transcript stability and selective protein translation/degradation also restricts the Hoxb4 expression domain. Transgenic mouse reporter analysis with defined regulatory element deletions, in situ hybridization, developmental time-course analysis Development (Cambridge, England) Medium 12736215
2002 An intron enhancer of mouse Hoxb4 requires binding by NFY (at an NFY/YY1 overlapping motif) for its activity in establishing the anterior boundary of mesodermal expression; specific mutations abolishing NFY binding eliminate enhancer activity. The same NFY/YY1 motif is present in the Hoxb4 promoter, suggesting relative NFY vs YY1 occupancy as a mechanism for balancing activation and repression. Heterologous promoter-reporter assays, site-directed mutagenesis of NFY and YY1 binding sites, transgenic mouse assay for anterior boundary of mesodermal expression Development (Cambridge, England) Medium 12135926
1994 The Hoxb-4 promoter contains multiple positive and negative regulatory elements: two cell-type-specific negative regulatory elements (regions a and d), an additional negative element (region b), and a positive element binding a novel factor HoxTF at sequence GCCATTGG that is essential for efficient Hoxb-4 expression; two 12-bp initiator elements flank each of the two transcription start sites (P1 and P2). Nuclease S1 and primer extension mapping of transcription start sites, detailed promoter mutagenesis with CAT reporter assays in multiple cell lines Molecular and cellular biology Medium 7969151
1999 HOXB4 is identified as a 1,25-dihydroxyvitamin D3-inducible protein that binds to the MIE1 site in c-myc intron 1 and participates in blocking c-myc transcriptional elongation during HL-60 monocytic differentiation; the MIE1 and MIE2/MIE3 sites are required for D3-mediated suppression of c-myc, and this binding activity requires PKCβ signaling. CAT reporter assays with c-myc promoter constructs containing deletions/mutations of MIE sites, gel-shift/EMSA, protein identification of MIE1-binding protein as HOXB4, PKCβ antisense and pharmacological inhibition The Journal of biological chemistry Medium 10085075
2001 Antisense knockdown of HOXB4 partially blocks 1,25-dihydroxyvitamin D3-mediated suppression of c-myc and inhibits HL-60 differentiation, establishing that endogenous HOXB4 is functionally required downstream of vitamin D3 signaling to suppress c-myc transcription elongation. Phosphorothioate antisense oligonucleotide knockdown of HOXB4, c-myc protein level measurement, HL-60 differentiation assays (NBT and non-specific esterase) The Journal of endocrinology Medium 11250656
2010 Hemogen (Hemgn), a hematopoietic-specific nuclear protein, is a direct transcriptional target of HOXB4: HOXB4 binds to the Hemgn promoter region both ex vivo and in vivo (ChIP). Overexpression of Hemgn partially recapitulates HOXB4-mediated myeloid progenitor expansion, and shRNA knockdown of Hemgn reduces HOXB4-mediated expansion. Tamoxifen-inducible HOXB4-ER(T2) system in lineage-negative murine bone marrow, expression microarrays, ChIP assay for HOXB4 binding to Hemgn promoter, Hemgn overexpression and shRNA knockdown with myeloid colony assays Blood High 20393131
2013 CUL4 ubiquitin ligase mediates post-translational degradation of HOXB4 protein through a conserved degradation signal sequence (degron) in the homeodomain, giving HOXB4 a short protein half-life (~1 hour). A CUL4-resistant (degradation-resistant) HOXB4 mutant has enhanced activity in expanding myeloid progenitors and better maintains human HSCs in a primitive state in vitro and in vivo. This degron is conserved in additional HOX paralogues. Co-immunoprecipitation of HOXB4 with CUL4, degron mapping by deletion mutagenesis, protein stability assays, engineered degradation-resistant HOXB4 variants, human HSC transduction and NOD/SCID/IL2Rγ-null mouse transplantation Blood High 23520338
2010 Hoxb4 transduction reduces accumulated Geminin protein in hematopoietic stem and progenitor cells through constituting an E3 ubiquitin ligase complex (RDCOXB4) with Roc1-Ddb1-Cul4a, targeting Geminin for ubiquitin-proteasome degradation; Geminin knockdown promotes clonogenic/replating activity while Geminin overexpression suppresses HOXB4-mediated HSC expansion. Co-immunoprecipitation of HOXB4 with Roc1-Ddb1-Cul4a complex in vitro and in vivo, ubiquitination assays, Geminin overexpression and knockdown, competitive repopulation in Rae28-deficient mice Proceedings of the National Academy of Sciences of the United States of America Medium 21098278
2013 USF1 directs recruitment of the hSET1A histone H3K4 methyltransferase complex to the HoxB4 promoter, establishing H3K4me3 marks and activating HoxB4 transcription during ESC differentiation toward hematopoietic lineages; disruption of USF1 or hSET1A function reduces HoxB4 expression and impairs mesoderm/hematopoietic differentiation. ChIP for H3K4me3 and USF1/hSET1A at HoxB4 promoter, dominant-negative USF1 overexpression, RNA interference knockdown of hSET1A, ESC differentiation assays PLoS genetics Medium 23754954
2003 The TALE homeodomain gene Irx5 is a direct, positively regulated transcriptional target of Hoxb4 in the developing chick embryo. Gain- and loss-of-function manipulation of Hoxb4 in chick embryos with direct readout of Irx5 expression Developmental dynamics Low 12701098
2004 Hoxb4 directly induces expression of FLASH (a component of the FAS-CASPASE8 apoptotic pathway) in the notochord after neurulation; blocking FLASH activity or Hoxb4 activity prevents apoptosis in the notochord. Gain- and loss-of-function manipulations of Hoxb4 and FLASH in developing embryos (chick), direct readout of FLASH expression and notochord apoptosis Developmental biology Low 14697356
2004 Mice homozygous for Hoxb4 deficiency exhibit mildly reduced hematopoietic organ cellularity and impaired proliferative responses of Lin-Sca1+c-kit+ bone marrow stem/progenitor cells in vitro and in vivo after transplantation, with altered expression of other Hox genes and cell cycle regulators. Hoxb4 is not required for HSC generation or steady-state hematopoiesis. Hoxb4 complete knockout mouse model, flow cytometry, competitive transplantation, in vitro culture proliferation, quantitative mRNA analysis, cell cycle assays Blood High 14962901
2003 Hoxb3/Hoxb4 double-deficient mice have reduced hematopoietic stem cell pool in fetal liver and impaired proliferative capacity of Lin-Sca1+c-kit+ progenitors in vitro and in vivo; slower cell cycle kinetics confer increased tolerance to anti-mitotic drugs, establishing a direct physiological role for Hoxb4 (with Hoxb3) in regulating stem cell proliferation and regeneration. Hoxb3/Hoxb4 compound knockout mice, competitive repopulation assay, homing assay, cytostatic drug challenge, flow cytometry, colony assays Molecular and cellular biology High 12748289
2007 HOXB4 activity protects adult HSCs from the detrimental anti-proliferative effects of TNF-α; FGF signaling interacts with HOXB4 activity in a context-dependent manner (FGF receptor inhibition enhances HOXB4-mediated expansion of adult and ES-derived HSCs, while FGF2 has a dominant-negative effect on the earliest hematopoietic cells). These findings establish that HOXB4 modulates HSC responses to extrinsic signals. Gene expression profiling of inducible HOXB4 in enriched adult HSCs and embryonic derivatives, functional assays with TNF-α treatment and FGF receptor inhibition, competitive repopulation Proceedings of the National Academy of Sciences of the United States of America Medium 17940039
2011 Genome-wide ChIP and expression profiling in ES-cell-derived HSPCs identifies Runx1, Scl/Tal1, Gata2, and Gfi1 as direct transcriptional targets of HoxB4, and reveals indirect regulation of Lmo2, Erg, Meis1, Pbx1, Nov, AhR, and Hemgn. HoxB4 acts predominantly as a transcriptional activator but context-dependently represses a significant subset of direct targets. ChIP-seq (ChIP coupled with deep sequencing) and genome-wide expression profiling in ES-derived hematopoietic stem/progenitor cells expressing inducible HoxB4 Blood Medium 21343615
2012 Dynamic ChIP-seq and gene expression profiling across four stages of HoxB4-mediated ESC differentiation toward HSCs reveals that the HoxB4 regulatory network expands progressively, HoxB4 co-regulates multiple hematopoietic transcription factors (Fli1, Meis1, Runx1, Scl) in distinct combinations, and down-regulation of mitochondrial and lysosomal genes by HoxB4 may contribute to impaired lymphoid development. ChIP coupled with deep sequencing and global gene expression profiling at 4 differentiation stages, joint bioinformatic analysis Blood Medium 22438249
2014 Prdm16 mRNA is markedly repressed as a direct component of the HOXB4 transcriptional program in transplanted HSCs; enforced co-expression of sPrdm16 with HOXB4 leads to enhanced self-renewal, myeloid expansion, and leukemia, establishing Prdm16 downregulation as a specific mechanism by which HOXB4-expanding HSCs avoid leukemic transformation. Time-course gene expression profiling of Lin-Sca1+c-kit+ cells from HOXB4-transplanted mice, co-overexpression of sPrdm16 with HOXB4 by retroviral transduction, murine transplantation leukemia model Blood Medium 25082879
2014 HoxB4 and STAT3 act in the same pathway for HSC self-renewal: simultaneous transduction of both does not produce additive effects; inhibition of STAT3 in HoxB4-overexpressing cells abrogates HoxB4 enhancement; HoxB4 upregulation causes ligand-independent Tyr-phosphorylation of STAT3; and the two transcription factors regulate significantly overlapping transcriptomes including pluripotency-related genes Oct-4 and Nanog. Retroviral co-transduction of STAT3-C and HoxB4, competitive repopulation assay, STAT3 pharmacological inhibition, phospho-STAT3 Western blot, microarray transcriptome analysis, gene set enrichment analysis Stem cells (Dayton, Ohio) Medium 24446131
2013 miR-23a directly binds the 3'UTR of HOXB4 mRNA and represses HOXB4 protein expression by ~65%, as demonstrated by luciferase reporter assay and Western blot. Luciferase 3'UTR reporter assay with miR-23a, Western blot for HOXB4 protein after miR-23a transfection Genes, chromosomes & cancer Medium 23630040
2016 A proline-rich sequence near the N-terminus of HOXB4, unique among HOX proteins and highly conserved in higher mammals, is required for controlled stem cell amplification: deletion of this domain substantially enhances HOXB4's oncogenicity causing acute leukemia in mice; insertion of the domain into HOXA9 impairs HOXA9's leukemogenicity. Domain deletion and domain-swap mutagenesis of HOXB4/HOXA9, retroviral transduction into murine bone marrow, transplantation with leukemia monitoring, in vivo stem cell expansion assays Blood High 27827825
2019 IGF2BP1 maintains HOXB4 mRNA stability in leukemia cells; IGF2BP1 inhibition reduces HOXB4 expression and decreases leukemia cell tumorigenicity, myeloid differentiation block, and chemotherapy resistance. CLIP and PAR-CLIP to identify HOXB4 as an IGF2BP1 RNA target, gain- and loss-of-function systems for IGF2BP1 in leukemia cell lines, tumor-initiating potential assays Leukemia Medium 31768017
2021 HOXB4 directly transcriptionally represses β-catenin expression, thereby inactivating the Wnt/β-catenin signaling pathway in cervical cancer cells; re-expression of β-catenin rescues HOXB4-induced growth inhibition. ChIP or reporter assays for HOXB4 binding to β-catenin promoter, gain/loss-of-function of HOXB4 in cervical cancer cells, β-catenin rescue experiment, nude mouse xenograft Cell death & disease Medium 33479226
2020 HOXB4 activates DHDDS transcription by binding to two DNA motifs in the DHDDS gene, contributing to ovarian cancer proliferation and invasion; HOXB4 also induces Snail and Zeb1 expression (EMT markers). ChIP assay for HOXB4 binding to DHDDS promoter motifs, HOXB4 gain/loss-of-function in ovarian cancer cells, xenograft mouse model BMC cancer Medium 32178630
2024 HOXB4 binds to the LINC00629 promoter and transcriptionally represses LINC00629 expression in ovarian cancer cells. ChIP assay for HOXB4 binding to the LINC00629 promoter, correlation of HOXB4 expression with LINC00629 levels Cancer science Low 38182548
2021 HOXB4 serves as a transcriptional activator of AKR1C3 and can suppress erastin-induced ferroptosis in H9C2 cardiomyocytes. Luciferase reporter assay for HOXB4 transactivation of AKR1C3, ferroptosis characteristic measurements (GPX4, MDA, iron, GSH) in HOXB4-transfected H9C2 cells Frontiers in cardiovascular medicine Low 34568444
2018 HOXB4 promotes hemogenic endothelium formation from differentiating mouse ESCs without altering endothelial cell development; whole-transcriptome analysis shows HOXB4 upregulates core hematopoietic transcription factors at the hemogenic endothelium stage, with blood progenitor formation requiring subsequent Runx1 expression. Retroviral HOXB4 expression in Runx1-/- ESCs with doxycycline-inducible Runx1, flow cytometric identification of hemogenic endothelium, whole-transcriptome analysis Stem cell reports Medium 29456178
2008 HOXB4 overexpression in large animals (dogs, macaques) caused myeloid leukemia ~2 years post-transplantation with dysregulated oncogene expression and a block in myeloid differentiation; HOXB4 knockdown in leukemic cells restored differentiation, suggesting direct HOXB4 involvement. Control gammaretroviral vectors (expressing MGMT or no transgene) did not cause leukemia. Retroviral HOXB4 transduction in large animal HSCs (dog, macaque), long-term transplantation monitoring, leukemia characterization (blast immunophenotyping, oncogene expression profiling), HOXB4 shRNA knockdown of leukemic cells The Journal of clinical investigation Medium 18357342

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 HoxB4 confers definitive lymphoid-myeloid engraftment potential on embryonic stem cell and yolk sac hematopoietic progenitors. Cell 601 11955444
2002 HOXB4-induced expansion of adult hematopoietic stem cells ex vivo. Cell 520 11955445
1995 Overexpression of HOXB4 in hematopoietic cells causes the selective expansion of more primitive populations in vitro and in vivo. Genes & development 460 7622039
2005 Generation of hematopoietic repopulating cells from human embryonic stem cells independent of ectopic HOXB4 expression. The Journal of experimental medicine 232 15883170
2003 In vitro expansion of hematopoietic stem cells by recombinant TAT-HOXB4 protein. Nature medicine 230 14578881
1998 Initiation of rhombomeric Hoxb4 expression requires induction by somites and a retinoid pathway. Neuron 226 9697850
2003 Ex vivo expansion of human hematopoietic stem cells by direct delivery of the HOXB4 homeoprotein. Nature medicine 214 14578882
1995 Compound mutants for the paralogous hoxa-4, hoxb-4, and hoxd-4 genes show more complete homeotic transformations and a dose-dependent increase in the number of vertebrae transformed. Genes & development 206 7628700
2001 HOXB4 overexpression mediates very rapid stem cell regeneration and competitive hematopoietic repopulation. Experimental hematology 168 11532354
1996 Targeted disruption of the Hoxb-2 locus in mice interferes with expression of Hoxb-1 and Hoxb-4. Development (Cambridge, England) 120 9012503
2019 RNA-binding protein IGF2BP1 maintains leukemia stem cell properties by regulating HOXB4, MYB, and ALDH1A1. Leukemia 118 31768017
2002 High-level ectopic HOXB4 expression confers a profound in vivo competitive growth advantage on human cord blood CD34+ cells, but impairs lymphomyeloid differentiation. Blood 118 12406897
1999 Enhanced in vivo regenerative potential of HOXB4-transduced hematopoietic stem cells with regulation of their pool size. Blood 113 10515864
2004 Hoxb4-deficient mice undergo normal hematopoietic development but exhibit a mild proliferation defect in hematopoietic stem cells. Blood 101 14962901
2002 Deregulated expression of HOXB4 enhances the primitive growth activity of human hematopoietic cells. Blood 96 12130496
2003 Reduced proliferative capacity of hematopoietic stem cells deficient in Hoxb3 and Hoxb4. Molecular and cellular biology 94 12748289
1996 Overexpression of HOXB4 enhances the hematopoietic potential of embryonic stem cells differentiated in vitro. Blood 94 8639890
2008 High incidence of leukemia in large animals after stem cell gene therapy with a HOXB4-expressing retroviral vector. The Journal of clinical investigation 91 18357342
2003 Thrombopoietin stimulates Hoxb4 expression: an explanation for the favorable effects of TPO on hematopoietic stem cells. Blood 84 12855555
2007 HOXB4's road map to stem cell expansion. Proceedings of the National Academy of Sciences of the United States of America 77 17940039
1998 Cellular proliferation and transformation induced by HOXB4 and HOXB3 proteins involves cooperation with PBX1. Oncogene 72 9692548
2005 HOXB4 enforces equivalent fates of ES-cell-derived and adult hematopoietic cells. Proceedings of the National Academy of Sciences of the United States of America 71 16093308
2003 The competitive nature of HOXB4-transduced HSC is limited by PBX1: the generation of ultra-competitive stem cells retaining full differentiation potential. Immunity 65 12705858
2003 NF-Y cooperates with USF1/2 to induce the hematopoietic expression of HOXB4. Blood 63 12791656
2006 HOXB4 overexpression promotes hematopoietic development by human embryonic stem cells. Stem cells (Dayton, Ohio) 59 16410392
2001 Retroviral vector-mediated expression of HoxB4 in hematopoietic cells using a novel coexpression strategy. Gene therapy 57 11420646
2013 USF1 and hSET1A mediated epigenetic modifications regulate lineage differentiation and HoxB4 transcription. PLoS genetics 52 23754954
2004 Molecular interactions involved in HOXB4-induced activation of HSC self-renewal. Blood 52 15226173
1995 Comparative analysis of chicken Hoxb-4 regulation in transgenic mice. Mechanisms of development 51 8555111
2006 Stem cell c-KIT and HOXB4 genes: critical roles and mechanisms in self-renewal, proliferation, and differentiation. Stem cells and development 47 17253940
2015 Activation of OCT4 enhances ex vivo expansion of human cord blood hematopoietic stem and progenitor cells by regulating HOXB4 expression. Leukemia 46 26202933
2002 Spatially specific expression of Hoxb4 is dependent on the ubiquitous transcription factor NFY. Development (Cambridge, England) 46 12135926
2011 Genome-wide analysis of target genes regulated by HoxB4 in hematopoietic stem and progenitor cells developing from embryonic stem cells. Blood 44 21343615
2012 Dynamic HoxB4-regulatory network during embryonic stem cell differentiation to hematopoietic cells. Blood 43 22438249
2007 Hematopoiesis and immunity of HOXB4-transduced embryonic stem cell-derived hematopoietic progenitor cells. Blood 43 18056836
2009 Cytoplasmic Prep1 interacts with 4EHP inhibiting Hoxb4 translation. PloS one 41 19365557
2003 Multiple levels of transcriptional and post-transcriptional regulation are required to define the domain of Hoxb4 expression. Development (Cambridge, England) 41 12736215
1999 c-myc intron element-binding proteins are required for 1, 25-dihydroxyvitamin D3 regulation of c-myc during HL-60 cell differentiation and the involvement of HOXB4. The Journal of biological chemistry 41 10085075
2000 Hematopoietic expression of HOXB4 is regulated in normal and leukemic stem cells through transcriptional activation of the HOXB4 promoter by upstream stimulating factor (USF)-1 and USF-2. The Journal of experimental medicine 39 11085749
2001 Hoxb2 and hoxb4 act together to specify ventral body wall formation. Developmental biology 38 11518511
2016 HOXB4 knockdown reverses multidrug resistance of human myelogenous leukemia K562/ADM cells by downregulating P-gp, MRP1 and BCRP expression via PI3K/Akt signaling pathway. International journal of oncology 37 27779650
2006 Differential effects of HOXB4 on nonhuman primate short- and long-term repopulating cells. PLoS medicine 37 16637742
2010 Downstream targets of HOXB4 in a cell line model of primitive hematopoietic progenitor cells. Blood 35 20404135
2021 HOXB4 inhibits the proliferation and tumorigenesis of cervical cancer cells by downregulating the activity of Wnt/β-catenin signaling pathway. Cell death & disease 34 33479226
2010 Hemgn is a direct transcriptional target of HOXB4 and induces expansion of murine myeloid progenitor cells. Blood 34 20393131
2003 Enforced adenoviral vector-mediated expression of HOXB4 in human umbilical cord blood CD34+ cells promotes myeloid differentiation but not proliferation. Molecular therapy : the journal of the American Society of Gene Therapy 33 14529835
2007 Hoxa9/hoxb3/hoxb4 compound null mice display severe hematopoietic defects. Experimental hematology 32 17761289
2013 Improved ex vivo expansion of adult hematopoietic stem cells by overcoming CUL4-mediated degradation of HOXB4. Blood 31 23520338
2005 HOXB4-induced self-renewal of hematopoietic stem cells is significantly enhanced by p21 deficiency. Stem cells (Dayton, Ohio) 31 16210402
1994 Multiple positive and negative regulatory elements in the promoter of the mouse homeobox gene Hoxb-4. Molecular and cellular biology 30 7969151
2010 Hoxb4 transduction down-regulates Geminin protein, providing hematopoietic stem and progenitor cells with proliferation potential. Proceedings of the National Academy of Sciences of the United States of America 29 21098278
2002 HOXB4 homeodomain protein is expressed in developing epidermis and skin disorders and modulates keratinocyte proliferation. Developmental dynamics : an official publication of the American Association of Anatomists 29 11984874
2011 Hoxb4-YFP reporter mouse model: a novel tool for tracking HSC development and studying the role of Hoxb4 in hematopoiesis. Blood 27 21278354
2010 Combination of HOXB4 and Delta-1 ligand improves expansion of cord blood cells. Blood 26 20921340
2012 HOXB4 can enhance the differentiation of embryonic stem cells by modulating the hematopoietic niche. Stem cells (Dayton, Ohio) 25 22084016
2012 HOXC4 homeoprotein efficiently expands human hematopoietic stem cells and triggers similar molecular alterations as HOXB4. Haematologica 25 22298821
2007 Effects of HOXB4 overexpression on ex vivo expansion and immortalization of hematopoietic cells from different species. Stem cells (Dayton, Ohio) 25 17510218
2004 Enhanced in vivo selection of bone marrow cells by retroviral-mediated coexpression of mutant O6-methylguanine-DNA-methyltransferase and HOXB4. Molecular therapy : the journal of the American Society of Gene Therapy 25 15509504
2008 Forced expression of HoxB4 enhances hematopoietic differentiation by human embryonic stem cells. Molecules and cells 24 18511880
2005 Control of self-renewal and differentiation of hematopoietic stem cells: HOXB4 on the threshold. Annals of the New York Academy of Sciences 24 15958692
2008 Lentiviral-mediated HoxB4 expression in human embryonic stem cells initiates early hematopoiesis in a dose-dependent manner but does not promote myeloid differentiation. Stem cells (Dayton, Ohio) 23 18617691
2014 Downregulation of Prdm16 mRNA is a specific antileukemic mechanism during HOXB4-mediated HSC expansion in vivo. Blood 22 25082879
2009 HOXB4-transduced embryonic stem cell-derived Lin-c-kit+ and Lin-Sca-1+ hematopoietic progenitors express H60 and are targeted by NK cells. Journal of immunology (Baltimore, Md. : 1950) 22 19828634
2007 Recombinant HoxB4 fusion proteins enhance hematopoietic differentiation of human embryonic stem cells. Stem cells and development 22 17784829
2018 HOXB4 knockdown enhances the cytotoxic effect of paclitaxel and cisplatin by downregulating ABC transporters in ovarian cancer cells. Gene 21 29660518
2021 AKR1C3 and Its Transcription Factor HOXB4 Are Promising Diagnostic Biomarkers for Acute Myocardial Infarction. Frontiers in cardiovascular medicine 20 34568444
2016 Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells. Stem cells translational medicine 20 27352929
2009 Ectopic HOXB4 overcomes the inhibitory effect of tumor necrosis factor-{alpha} on Fanconi anemia hematopoietic stem and progenitor cells. Blood 20 19270262
2003 TALE class homeodomain gene Irx5 is an immediate downstream target for Hoxb4 transcriptional regulation. Developmental dynamics : an official publication of the American Association of Anatomists 20 12701098
2018 HOXB4 Promotes Hemogenic Endothelium Formation without Perturbing Endothelial Cell Development. Stem cell reports 18 29456178
2012 The HOXB4 homeoprotein promotes the ex vivo enrichment of functional human embryonic stem cell-derived NK cells. PloS one 18 22761810
2006 HOXB4 inhibits cell growth in a dose-dependent manner and sensitizes cells towards extrinsic cues. Cell cycle (Georgetown, Tex.) 18 16357528
2004 FLASH, a component of the FAS-CAPSASE8 apoptotic pathway, is directly regulated by Hoxb4 in the notochord. Developmental biology 18 14697356
2001 Antisense knockout of HOXB4 blocks 1,25-dihydroxyvitamin D3 inhibition of c-myc expression. The Journal of endocrinology 18 11250656
2014 Molecular integration of HoxB4 and STAT3 for self-renewal of hematopoietic stem cells: a model of molecular convergence for stemness. Stem cells (Dayton, Ohio) 17 24446131
2008 Unique spatial and cellular expression patterns of Hoxa5, Hoxb4, and Hoxb6 proteins in normal developing murine lung are modified in pulmonary hypoplasia. Birth defects research. Part A, Clinical and molecular teratology 17 18553509
2013 Identification of the transcription factor HOXB4 as a novel target of miR-23a. Genes, chromosomes & cancer 16 23630040
2012 Mechanism of action of HOXB4 on the hematopoietic differentiation of embryonic stem cells. Stem cells (Dayton, Ohio) 16 22267295
1999 RARbeta mediates the response of Hoxd4 and Hoxb4 to exogenous retinoic acid. Developmental dynamics : an official publication of the American Association of Anatomists 16 10373014
2011 Promotion of hematopoietic differentiation from mouse induced pluripotent stem cells by transient HoxB4 transduction. Stem cell research 15 22000550
2008 HOXB4 but not BMP4 confers self-renewal properties to ES-derived hematopoietic progenitor cells. Transplantation 15 19104426
2007 The HOXB4 homeoprotein differentially promotes ex vivo expansion of early human lymphoid progenitors. Stem cells (Dayton, Ohio) 15 17962697
2010 Induction of hematopoietic differentiation of mouse embryonic stem cells by an AGM-derived stromal cell line is not further enhanced by overexpression of HOXB4. Stem cells and development 14 20184433
2010 Ex vivo expansion of human HSCs with Sendai virus vector expressing HoxB4 assessed by sheep in utero transplantation. Experimental hematology 14 20875838
2005 HOXB4 confers a constant rate of in vitro proliferation to transduced bone marrow cells. Oncogene 14 15580301
2016 Controlled stem cell amplification by HOXB4 depends on its unique proline-rich region near the N terminus. Blood 13 27827825
2010 Successful treatment of metachromatic leukodystrophy using bone marrow transplantation of HoxB4 overexpressing cells. Molecular therapy : the journal of the American Society of Gene Therapy 13 20424597
2008 Expansion of haematopoietic stem cells from normal donors and bone marrow failure patients by recombinant hoxb4. British journal of haematology 13 19120358
2021 Overexpression of HOXB4 Promotes Protection of Bone Marrow Mesenchymal Stem Cells Against Lipopolysaccharide-Induced Acute Lung Injury Partially Through the Activation of Wnt/β-Catenin Signaling. Journal of inflammation research 12 34349541
2020 HOXB4 promotes the malignant progression of ovarian cancer via DHDDS. BMC cancer 12 32178630
2018 MiR-10a and HOXB4 are overexpressed in atypical myeloproliferative neoplasms. BMC cancer 12 30419846
2014 Co-transplantation with mesenchymal stem cells expressing a SDF-1/HOXB4 fusion protein markedly improves hematopoietic stem cell engraftment and hematogenesis in irradiated mice. American journal of translational research 12 25628780
2010 HoxB2, HoxB4 and Alx4 genes are downregulated in the cadmium-induced omphalocele in the chick model. Pediatric surgery international 12 20625746
2017 Functional analysis of HOXA10 and HOXB4 in human medulloblastoma cell lines. International journal of oncology 11 29039487
2008 Complementary and independent function for Hoxb4 and Bmi1 in HSC activity. Cold Spring Harbor symposia on quantitative biology 11 19022763
2004 Hoxb4 in oligodendrogenesis. Cellular and molecular neurobiology 11 15206819
2012 The functional duality of HoxB4 in hematopoietic reconstituting cells. Cytometry. Part A : the journal of the International Society for Analytical Cytology 10 22522741
2024 LINC00629, a HOXB4-downregulated long noncoding RNA, inhibits glycolysis and ovarian cancer progression by destabilizing c-Myc. Cancer science 9 38182548
2020 Activated HoxB4-induced hematopoietic stem cells from murine pluripotent stem cells via long-term programming. Experimental hematology 9 32795499
2017 HOXB4 Immunoreactivity in Endometrial Tissues From Women With or Without Endometriosis. Reproductive sciences (Thousand Oaks, Calif.) 9 28969513

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