Affinage

FLOT2

Flotillin-2 · UniProt Q14254

Length
428 aa
Mass
47.1 kDa
Annotated
2026-04-28
100 papers in source corpus 15 papers cited in narrative 15 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

FLOT2 is a lipid raft scaffold protein that organizes membrane microdomains to coordinate signaling, cytoskeletal remodeling, and protein stability across diverse cell types. Through its SPFH domain, FLOT2 recruits podocin and nephrin into lipid rafts in podocytes and directly protects synaptopodin from K48-linked polyubiquitination-mediated proteasomal degradation, functioning as a critical guardian of podocyte slit diaphragm integrity (PMID:36632460, PMID:40681143). FLOT2 also scaffolds cytoneme formation together with the Wnt co-receptor Ror2 to mediate intercellular Wnt transport (PMID:36040316), interacts with LAMP2 to promote autophagosome–lysosome fusion (PMID:40066518), and stabilizes EphA2 and STAT3 to activate Akt, NF-κB, and Raf/MEK/ERK signaling cascades that drive proliferation and metastasis in multiple cancer contexts (PMID:33631676, PMID:28560058, PMID:38161417). FLOT2 mRNA stability is regulated by m6A modifications deposited by METTL14 and removed by FTO, with recognition by IGF2BP3 and HNRNPH1 sustaining transcript levels (PMID:39570559, PMID:38914215).

Mechanistic history

Synthesis pass · year-by-year structured walk · 10 steps
  1. 1994 Medium

    Identification of FLOT2 as a broadly expressed ~42 kDa membrane-associated protein with a hydrophobic N-terminus and alpha-helical C-terminus established the gene's basic molecular identity and tissue distribution.

    Evidence cDNA cloning, Northern blot, sequence analysis, and chromosomal mapping in keratinocytes, fibroblasts, and carcinoma lines

    PMID:8051082

    Open questions at the time
    • No functional role defined
    • Lipid raft association not yet demonstrated
    • No interacting partners identified
  2. 1999 Medium

    Overexpression of FLOT2 induced filopodia formation and neuronal-like morphology, establishing a functional link to actin cytoskeleton remodeling and cellular protrusions.

    Evidence Stable FLOT2-EGFP transfection in COS and epithelial cells with morphological analysis

    PMID:10462713

    Open questions at the time
    • Gain-of-function only; no loss-of-function validation
    • Mechanism linking FLOT2 to actin regulation unknown
    • Endogenous relevance not shown
  3. 2017 Medium

    FLOT2 was shown to activate the Raf/MEK/ERK cascade and to promote EMT via Twist upregulation in hepatocellular carcinoma, defining a specific oncogenic signaling axis downstream of FLOT2.

    Evidence Overexpression/siRNA knockdown with Western blot pathway analysis and in vivo xenograft models in HCC

    PMID:28560058

    Open questions at the time
    • Direct mechanism by which FLOT2 activates Raf unknown
    • Whether lipid raft scaffolding is required for ERK activation not tested
  4. 2019 Medium

    Fyn kinase was identified as a regulator of FLOT2 lipid raft localization, with both proteins co-localizing in detergent-resistant membranes during neural differentiation, establishing that post-translational phosphorylation controls FLOT2 membrane partitioning.

    Evidence Sucrose density gradient fractionation, immunofluorescence, and Western blot during P19C6 neural differentiation

    PMID:31455216

    Open questions at the time
    • Fyn phosphorylation sites on FLOT2 not mapped
    • Functional consequence of Fyn-mediated FLOT2 phosphorylation not established
    • Podocyte relevance inferred but not directly tested
  5. 2021 Medium

    FLOT2 was shown to physically interact with and stabilize EphA2 receptor tyrosine kinase, activating Akt and NF-κB in glioma, revealing a mechanism by which FLOT2 scaffolds receptor stabilization to promote oncogenic signaling.

    Evidence Reciprocal Co-IP, siRNA knockdown, EphA2 functional rescue, proliferation and invasion assays

    PMID:33631676

    Open questions at the time
    • Whether FLOT2 prevents EphA2 degradation via ubiquitin-proteasome or lysosomal pathway not determined
    • Structural basis of interaction unknown
  6. 2022 High

    FLOT2 was established as a scaffold that, together with Ror2, determines cytoneme number and length for intercellular Wnt transport, validated in both cancer cells and zebrafish embryogenesis — revealing a conserved role in contact-dependent morphogen delivery.

    Evidence Live imaging, siRNA knockdown, co-localization, functional rescue in gastric cancer cells and zebrafish embryos

    PMID:36040316

    Open questions at the time
    • Molecular mechanism by which FLOT2-Ror2 nucleates cytoneme formation unresolved
    • Whether other flotillins compensate in vivo not tested
  7. 2022 Medium

    A TBL1X–TCF4–FLOT2–c-Myc positive feedback loop was identified in nasopharyngeal carcinoma metastasis, establishing that FLOT2 is both a transcriptional target of Wnt/β-catenin effectors and an upstream amplifier of c-Myc expression.

    Evidence ChIP, Co-IP, luciferase reporter assays, knockdown/overexpression, in vivo metastasis models

    PMID:35173544

    Open questions at the time
    • Direct mechanism by which FLOT2 upregulates c-Myc not defined
    • Whether this feedback loop operates outside NPC not tested
  8. 2023 High

    FLOT2 was demonstrated to recruit podocin and nephrin to lipid rafts through direct SPFH domain–SPFH domain interaction, and podocyte-specific Flot2 knockout worsened nephropathy while overexpression was protective — establishing FLOT2 as essential for podocyte slit diaphragm organization.

    Evidence Conditional KO and transgenic OE mice, Co-IP, SPFH domain mapping, sucrose gradient fractionation, LPS/ADR nephropathy models

    PMID:36632460

    Open questions at the time
    • Whether FLOT1 compensates or cooperates with FLOT2 in podocytes not addressed
    • Downstream signaling consequences of raft disruption in podocytes not fully characterized
  9. 2024 Medium

    Two independent studies revealed that FLOT2 mRNA stability is controlled by m6A epitranscriptomic regulation: FTO-mediated demethylation sustains FLOT2 mRNA in DLBCL, while HNRNPH1 stabilizes METTL14 to increase m6A on FLOT2 mRNA recognized by IGF2BP3 in NPC — establishing convergent post-transcriptional control of FLOT2 levels.

    Evidence RNA immunoprecipitation, m6A methylation assays, mRNA stability assays, functional rescue in DLBCL and NPC cell lines

    PMID:38914215 PMID:39570559

    Open questions at the time
    • Apparently contradictory effects of m6A on FLOT2 mRNA stability (FTO demethylation stabilizes in DLBCL vs. METTL14 methylation stabilizes in NPC) not reconciled
    • Specific m6A sites on FLOT2 mRNA not mapped
  10. 2025 High

    FLOT2 was shown to protect synaptopodin from K48-linked ubiquitin-mediated proteasomal degradation in podocytes, and in a parallel study to interact with LAMP2 to promote autophagosome–lysosome fusion in cardiac endothelial cells — broadening FLOT2's role to protein quality control and autophagic flux regulation.

    Evidence Co-IP, ubiquitination assays, podocyte-specific KO/OE in diabetic nephropathy mice; reciprocal Co-IP, CLP sepsis model, autophagy flux assays with FLOT2 KO

    PMID:40066518 PMID:40681143

    Open questions at the time
    • Whether FLOT2 competes with specific E3 ligases for synaptopodin binding not determined
    • Structural basis of FLOT2-LAMP2 interaction unknown
    • Whether FLOT2-LAMP2 interaction requires lipid raft context not tested

Open questions

Synthesis pass · forward-looking unresolved questions
  • How FLOT2 integrates its diverse scaffolding functions — lipid raft organization, cytoneme formation, receptor stabilization, ubiquitin protection, and autophagosome–lysosome fusion — into a unified molecular mechanism remains unresolved; no high-resolution structural model of full-length FLOT2 or its complexes exists.
  • No crystal or cryo-EM structure of FLOT2 or its complexes
  • Relative contributions of FLOT1-FLOT2 hetero-oligomerization vs. FLOT2 homo-oligomerization to function unclear
  • Cell-type-specific regulation of FLOT2 scaffolding activity not systematically defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4 GO:0008092 cytoskeletal protein binding 2
Localization
GO:0005886 plasma membrane 4 GO:0005764 lysosome 1
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-1266738 Developmental Biology 1 R-HSA-9612973 Autophagy 1
Partners
EPHA2FLOT1FYNLAMP2NPHS2ROR2STAT3SYNPO

Evidence

Reading pass · 15 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1994 FLOT2 (originally identified as ESA/epidermal surface antigen) encodes a ~42 kDa protein with an NH2-terminal hydrophobic region and hydrophilic COOH-terminal alpha-helix-rich region, conserved across mammalian species, and expressed in keratinocytes, melanocytes, fibroblasts, and carcinoma cell lines; its gene maps to human chromosome 17q11-12. cDNA cloning, sequence analysis, Northern blot, chromosomal localization The Journal of biological chemistry Medium 8051082
1999 Stable transfection of FLOT2-EGFP fusion protein in COS cells induced filopodia formation and caused epithelial cells to adopt a neuronal appearance, indicating that FLOT2 has a role in actin-based cellular protrusion and morphology changes similar to neuronal regeneration proteins Reggie 1 and 2. Stable transfection of EGFP-fusion protein, morphological analysis in COS cells Journal of cellular biochemistry Medium 10462713
2017 LXR agonist treatment decreased mRNA and protein expression of FLOT2 and its palmitoyl transferase DHHC5 in MCF-7 breast cancer cells, and also reduced Akt phosphorylation and its localization at the plasma membrane, linking FLOT2-containing lipid rafts to PI3K/Akt signaling. LXR agonist T0901317 treatment, RT-PCR, Western blot, subcellular fractionation Anticancer research Low 28739689
2019 FLOT2 is expressed in podocytes and its lipid raft localization is regulated by Fyn kinase (a Src family kinase); Flot2 and Fyn protein levels increase during neural differentiation of P19C6 cells, with both proteins co-localizing in lipid rafts (detergent-resistant membrane fractions) following differentiation, and Fyn phosphorylates Flot2 during this process. Sucrose density gradient fractionation, immunofluorescence, Western blot during neural differentiation of P19C6 cells BMC molecular and cell biology Medium 31455216
2021 FLOT2 promotes glioma growth and invasion by interacting with and stabilizing EphA2 receptor tyrosine kinase; FLOT2 depletion suppressed Akt and NF-κB activities and induced apoptosis, and restoration of EphA2 reversed the suppressive effects of FLOT2 knockdown. Co-IP, Western blot, siRNA knockdown, cell proliferation and invasion assays, EphA2 rescue experiments Biochemical and biophysical research communications Medium 33631676
2022 FLOT2 acts as a scaffold protein that, together with the Wnt co-receptor Ror2, determines the number and length of cytonemes used to transport Wnt3 intercellularly in gastric cancer cells; Flot2 knockdown reduced cytoneme formation and impaired Wnt3-dependent proliferation and cell survival. The same mechanism was observed for Wnt8a cytonemes during zebrafish embryogenesis. Live imaging of cytonemes, siRNA knockdown, FLOT2/Ror2 co-localization, functional rescue, zebrafish embryogenesis experiments eLife High 36040316
2022 TBL1X interacts with TCF4 to transcriptionally activate Flot2 expression; Flot2 in turn upregulates TBL1X by increasing c-myc (a positive transcriptional regulator of TBL1X), forming a positive feedback loop that promotes nasopharyngeal carcinoma metastasis. Co-IP, ChIP, luciferase reporter assays, knockdown/overexpression functional assays, in vivo metastasis models International journal of biological sciences Medium 35173544
2023 FLOT2 is required for recruitment of podocin and nephrin into lipid rafts in podocytes; FLOT2 directly interacts with podocin via their SPFH domains. Podocyte-specific Flot2 deletion worsened albuminuria and podocyte injury in LPS/ADR-induced nephropathy mouse models, while podocyte-specific Flot2 overexpression was protective. Podocyte-specific KO and transgenic overexpression mice, Co-IP, SPFH domain interaction mapping, sucrose gradient fractionation, in vivo nephropathy models International journal of biological sciences High 36632460
2023 FLOT2 promotes NPC progression by stabilizing STAT3 via protein interaction, which in turn drives CD109 expression to suppress TGF-β/Smad signaling, thereby promoting tumor growth and metastasis. Co-IP, immunofluorescence co-localization, ChIP, dual-luciferase assay, RNA-seq, in vitro and in vivo functional assays iScience Medium 38161417
2024 HNRNPH1 maintains FLOT2 mRNA stability through an m6A-dependent mechanism: HNRNPH1 interacts with METTL14 to prevent its STUB1-mediated ubiquitin degradation, leading to increased m6A modification of FLOT2 mRNA by METTL14, which is then recognized by IGF2BP3 to further stabilize FLOT2 mRNA in nasopharyngeal carcinoma. RNA immunoprecipitation, Co-IP, m6A methylation analysis, knockdown/overexpression experiments, mRNA stability assays Cellular oncology Medium 39570559
2024 FTO-mediated m6A demethylation upregulates FLOT2 by sustaining FLOT2 mRNA stability; elevated FLOT2 subsequently activates PI3K/Akt/mTOR signaling to promote DLBCL aggressiveness. Rescue experiments confirmed that FTO-ablation effects were abrogated by FLOT2 overexpression. m6A methylation analysis, Western blot, overexpression/silencing vectors, functional rescue experiments, PI3K/Akt/mTOR pathway analysis Archives of biochemistry and biophysics Medium 38914215
2025 FLOT2 directly interacts with synaptopodin and protects it from K48-linked polyubiquitination-mediated proteasomal degradation in podocytes; loss of Flot2 in diabetic mice worsened podocyte injury and renal dysfunction, while podocyte-specific Flot2 overexpression was protective in diabetic nephropathy. Co-IP, ubiquitination assays, podocyte-specific KO and OE transgenic mice, diabetic nephropathy models, Western blot Metabolism: clinical and experimental High 40681143
2025 FLOT2 interacts with LAMP2 (lysosomal-associated membrane protein 2) in cardiac endothelial cells upon IL4 stimulation; this interaction enhances autophagosome-lysosome fusion and rescues impaired autophagic flux during sepsis. Loss of FLOT2 reversed the LAMP2-mediated effects on autophagy, causing autophagosome accumulation. Co-IP, CLP mouse sepsis model, autophagy flux assays (LC3, p62, acridine orange), FLOT2 KO, IL4 treatment, cardiac function assessment Autophagy High 40066518
2017 Flot2 promotes HCC tumor growth and metastasis by modulating cell cycle progression and inducing EMT via up-regulation of Twist as a result of Raf/MEK/ERK1/2 pathway activation; forced Flot2 overexpression promoted HCC proliferation and metastasis in vitro and in vivo, while Flot2 silencing inhibited these processes. Overexpression and siRNA knockdown, Western blot for Raf/MEK/ERK pathway, EMT markers, in vivo xenograft models American journal of cancer research Medium 28560058
2024 PAF1 transcriptionally regulates FLOT2 expression in cervical cancer, and FLOT2 mediates the oncogenic effects of PAF1 through the MEK/ERK1/2 pathway; PAF1 knockdown reduced FLOT2 expression and suppressed tumor growth in vivo, effects that were reversed by FLOT2 overexpression. siRNA knockdown, Western blot, in vivo tumor models, rescue experiments Cell adhesion & migration Low 37754347

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Acetylation of histone H4 by Esa1 is required for DNA double-strand break repair. Nature 456 12353039
1998 ESA1 is a histone acetyltransferase that is essential for growth in yeast. Proceedings of the National Academy of Sciences of the United States of America 279 9520405
2000 Coordinate regulation of yeast ribosomal protein genes is associated with targeted recruitment of Esa1 histone acetylase. Molecular cell 239 11163204
2003 Yeast enhancer of polycomb defines global Esa1-dependent acetylation of chromatin. Genes & development 177 12782659
2000 Crystal structure of yeast Esa1 suggests a unified mechanism for catalysis and substrate binding by histone acetyltransferases. Molecular cell 144 11106757
2002 The catalytic mechanism of the ESA1 histone acetyltransferase involves a self-acetylated intermediate. Nature structural biology 135 12368900
2009 NuA4 lysine acetyltransferase Esa1 is targeted to coding regions and stimulates transcription elongation with Gcn5. Molecular and cellular biology 90 19822662
2013 The challenging environment on board the International Space Station affects endothelial cell function by triggering oxidative stress through thioredoxin interacting protein overexpression: the ESA-SPHINX experiment. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 86 23913861
2012 Novel fusion of MYST/Esa1-associated factor 6 and PHF1 in endometrial stromal sarcoma. PloS one 81 22761769
2019 Gcn5 and Esa1 function as histone crotonyltransferases to regulate crotonylation-dependent transcription. The Journal of biological chemistry 76 31699900
2019 MicroRNA-351 eases insulin resistance and liver gluconeogenesis via the PI3K/AKT pathway by inhibiting FLOT2 in mice of gestational diabetes mellitus. Journal of cellular and molecular medicine 68 31287224
2019 Limits of Life and the Habitability of Mars: The ESA Space Experiment BIOMEX on the ISS. Astrobiology 66 30742496
2002 Esa1, an Arabidopsis mutant with enhanced susceptibility to a range of necrotrophic fungal pathogens, shows a distorted induction of defense responses by reactive oxygen generating compounds. The Plant journal : for cell and molecular biology 64 11862946
2009 Expression of CD133, PAX2, ESA, and GPR30 in invasive ductal breast carcinomas. Chinese medical journal 63 19951611
2014 High-volume online haemodiafiltration improves erythropoiesis-stimulating agent (ESA) resistance in comparison with low-flux bicarbonate dialysis: results of the REDERT study. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 59 25385719
2014 Acetylome profiling reveals overlap in the regulation of diverse processes by sirtuins, gcn5, and esa1. Molecular & cellular proteomics : MCP 54 25381059
2008 Bisubstrate Inhibitors of the MYST HATs Esa1 and Tip60. Bioorganic & medicinal chemistry 54 19114310
2016 MiR-485 inhibits metastasis and EMT of lung adenocarcinoma by targeting Flot2. Biochemical and biophysical research communications 48 27262438
2008 Impact of elevated C-reactive protein levels on erythropoiesis- stimulating agent (ESA) dose and responsiveness in hemodialysis patients. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 48 18840893
2011 Expression of CD44, CD24 and ESA in pancreatic adenocarcinoma cell lines varies with local microenvironment. Hepatobiliary & pancreatic diseases international : HBPD INT 42 21813394
2010 Discovery and basic pharmacology of erythropoiesis-stimulating agents (ESAs), including the hyperglycosylated ESA, darbepoetin alfa: an update of the rationale and clinical impact. European journal of clinical pharmacology 41 20127232
2013 Distinct and redundant roles of the two MYST histone acetyltransferases Esa1 and Sas2 in cell growth and morphogenesis of Candida albicans. Eukaryotic cell 40 23355007
2015 miR-449a targets Flot2 and inhibits gastric cancer invasion by inhibiting TGF-β-mediated EMT. Diagnostic pathology 39 26576674
2014 Identification of FLOT2 as a novel target for microRNA-34a in melanoma. Journal of cancer research and clinical oncology 39 25403318
2008 Novel structural and functional mode of a knot essential for RNA binding activity of the Esa1 presumed chromodomain. Journal of molecular biology 35 18407291
2022 Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2. International journal of oncology 34 35929514
1999 Flotillin 2 is distinct from epidermal surface antigen (ESA) and is associated with filopodia formation. Journal of cellular biochemistry 34 10462713
1994 Cloning and characterization of a novel epidermal cell surface antigen (ESA). The Journal of biological chemistry 33 8051082
2001 The cytotoxic effect of Eucheuma serra agglutinin (ESA) on cancer cells and its application to molecular probe for drug delivery system using lipid vesicles. Cytotechnology 32 19003319
2023 Fitness assessment of Mytilus galloprovincialis Lamarck, 1819 after exposure to herbicide metabolite propachlor ESA. Environmental pollution (Barking, Essex : 1987) 31 37236591
2009 Collaboration between the essential Esa1 acetyltransferase and the Rpd3 deacetylase is mediated by H4K12 histone acetylation in Saccharomyces cerevisiae. Genetics 31 19596907
2008 Catalytic-site mutations in the MYST family histone Acetyltransferase Esa1. Genetics 31 18245364
2017 Flot2 promotes tumor growth and metastasis through modulating cell cycle and inducing epithelial-mesenchymal transition of hepatocellular carcinoma. American journal of cancer research 29 28560058
2014 The ESA scenario gets complex: from biosimilar epoetins to activin traps. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 28 24748667
2024 Review of chemical models and applications in Geant4-DNA: Report from the ESA BioRad III Project. Medical physics 27 38889367
2020 MiR-7 reduces the BCSC subset by inhibiting XIST to modulate the miR-92b/Slug/ESA axis and inhibit tumor growth. Breast cancer research : BCR 27 32143670
2019 ESA1 Is Involved in Embryo Sac Abortion in Interspecific Hybrid Progeny of Rice. Plant physiology 27 30770460
2014 SiRNA-mediated flotillin-2 (Flot2) downregulation inhibits cell proliferation, migration, and invasion in gastric carcinoma cells. Oncology research 26 24854103
2010 Homocitrate synthase connects amino acid metabolism to chromatin functions through Esa1 and DNA damage. Genes & development 26 20810648
2019 Setting Fire to ESA and EMA Resistance: New Targeted Treatment Options in Lower Risk Myelodysplastic Syndromes. International journal of molecular sciences 25 31394818
2016 Regulation of oxidative phosphorylation through each-step activation (ESA): Evidences from computer modeling. Progress in biophysics and molecular biology 25 27939921
2012 Piccolo NuA4-catalyzed acetylation of nucleosomal histones: critical roles of an Esa1 Tudor/chromo barrel loop and an Epl1 enhancer of polycomb A (EPcA) basic region. Molecular and cellular biology 25 23109429
2020 Circ-FOXM1 contributes to cell proliferation, invasion, and glycolysis and represses apoptosis in melanoma by regulating miR-143-3p/FLOT2 axis. World journal of surgical oncology 24 32183822
2021 ESA1 regulates meiotic chromosome axis and crossover frequency via acetylating histone H4. Nucleic acids research 22 34417612
2020 Long non-coding RNA MIR4435-2HG recruits miR-802 from FLOT2 to promote melanoma progression. European review for medical and pharmacological sciences 22 32196611
2023 Next generation of astronauts or ESA astronaut 2.0 concept and spotlight on immunity. NPJ microgravity 20 37380641
2016 Hypogonadism associated with muscle atrophy, physical inactivity and ESA hyporesponsiveness in men undergoing haemodialysis. Nefrologia : publicacion oficial de la Sociedad Espanola Nefrologia 20 27553987
2019 The study of mechanism of miR-34c-5p targeting FLOT2 to regulate proliferation, migration and invasion of osteosarcoma cells. Artificial cells, nanomedicine, and biotechnology 19 31446795
2017 Chromatin Regulation by the NuA4 Acetyltransferase Complex Is Mediated by Essential Interactions Between Enhancer of Polycomb (Epl1) and Esa1. Genetics 19 28108589
2016 ESA Hyporesponsiveness Is Associated with Adverse Events in Maintenance Hemodialysis (MHD) Patients, But Not with Iron Storage. PloS one 19 26933949
2014 Red blood cell (RBC) transfusion rates among US chronic dialysis patients during changes to Medicare end-stage renal disease (ESRD) reimbursement systems and erythropoiesis stimulating agent (ESA) labels. BMC nephrology 18 25015348
1998 Expression, characterization and serological reactivity of a 41 kDa excreted-secreted antigen (ESA) from Toxoplasma gondii. Molecular and biochemical parasitology 18 9879891
2022 TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma. International journal of biological sciences 17 35173544
2021 FLOT2 upregulation promotes growth and invasion by interacting and stabilizing EphA2 in gliomas. Biochemical and biophysical research communications 17 33631676
2017 miR-133 involves in lung adenocarcinoma cell metastasis by targeting FLOT2. Artificial cells, nanomedicine, and biotechnology 17 28503944
2022 Egg cell-secreted aspartic proteases ECS1/2 promote gamete attachment to prioritize the fertilization of egg cells over central cells in Arabidopsis. Journal of integrative plant biology 16 36165344
2017 LXR Activation Down-regulates Lipid Raft Markers FLOT2 and DHHC5 in MCF-7 Breast Cancer Cells. Anticancer research 15 28739689
2012 Development and characterization of a human antibody reference panel against erythropoietin suitable for the standardization of ESA immunogenicity testing. Journal of immunological methods 15 22634015
2010 Population pharmacokinetic/pharmacodynamic model for C.E.R.A. in both ESA-naive and ESA-treated chronic kidney disease patients with renal anemia. Journal of clinical pharmacology 15 20179322
2001 Evaluation of inter-scorer and inter-laboratory reliability of the mouse epididymal sperm aneuploidy (m-ESA) assay. Mutagenesis 15 11320142
2015 C-reactive protein but not hepcidin, NGAL and transferrin determines the ESA resistance in hemodialysis patients. Renal failure 14 26539647
2014 Delta-He: a novel marker of inflammation predicting mortality and ESA response in peritoneal dialysis patients. Clinical kidney journal 14 25852889
2013 Unravelling the Neospora caninum secretome through the secreted fraction (ESA) and quantification of the discharged tachyzoite using high-resolution mass spectrometry-based proteomics. Parasites & vectors 14 24267406
2011 Detection of anti-ESA antibodies in human samples from PRCA and non-PRCA patients: an immunoassay platform comparison. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 14 21602184
2022 The scaffolding protein flot2 promotes cytoneme-based transport of wnt3 in gastric cancer. eLife 13 36040316
2015 Effects of OXPHOS complex deficiencies and ESA dysfunction in working intact skeletal muscle: implications for mitochondrial myopathies. Biochimica et biophysica acta 13 26188374
2020 Circ_001569 regulates FLOT2 expression to promote the proliferation, migration, invasion and EMT of osteosarcoma cells through sponging miR-185-5p. Open life sciences 12 33817236
2019 The fraction of CD117/c-KIT-expressing erythroid precursors predicts ESA response in low-risk myelodysplastic syndromes. Cytometry. Part B, Clinical cytometry 12 30963682
2015 The Set3 Complex Antagonizes the MYST Acetyltransferase Esa1 in the DNA Damage Response. Molecular and cellular biology 12 26303527
2012 A detailed examination of the antibody prevalence and characteristics of anti-ESA antibodies. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 12 23114907
2024 Fat mass and obesity-associated protein (FTO)-induced upregulation of flotillin-2 (FLOT2) contributes to cancer aggressiveness in diffuse large B-cell lymphoma (DLBCL) via activating the PI3K/Akt/mTOR signal pathway. Archives of biochemistry and biophysics 11 38914215
2018 Red blood cell volume is not decreased in ESA-naive anemic chronic kidney disease patients. Physiological reports 11 30426716
2019 The Flot2 component of the lipid raft changes localization during neural differentiation of P19C6 cells. BMC molecular and cell biology 10 31455216
2025 LAMP2-FLOT2 interaction enhances autophagosome-lysosome fusion to protect the septic heart in response to ILC2. Autophagy 9 40066518
2013 Effect of conversion from ESA with shorter half-life to CERA once monthly for maintaining Hb concentration in pre-dialysis CKD patients. Kidney & blood pressure research 9 24022228
1998 The ECS1 gene of Arabidopsis encodes a plant cell wall-associated protein and is potentially linked to a locus influencing resistance to Xanthomonas campestris. Plant molecular biology 9 9869403
2022 Intravenous iron versus oral iron versus no iron with or without erythropoiesis- stimulating agents (ESA) for cancer patients with anaemia: a systematic review and network meta-analysis. The Cochrane database of systematic reviews 8 35724934
2012 Suppression analysis of esa1 mutants in Saccharomyces cerevisiae links NAB3 to transcriptional silencing and nucleolar functions. G3 (Bethesda, Md.) 8 23050233
2008 [Clinical Practice guidelines for the use of erythropoiesis-stimulating agents (ESA: epoetin alfa, epoetin bêta, darbepoetin) in anaemic patients with cancer: 2007 update (summary report)]. Bulletin du cancer 8 18495573
2023 Effects and molecular mechanism of sugar transporter ESA_RS15745 on desiccation resistance, motility, and biofilm formation of Cronobacter sakazakii. Journal of food science 7 38126106
2016 Identification and characterization of an immunogenic antigen, enolase 2, among excretory/secretory antigens (ESA) of Toxoplasma gondii. Protein expression and purification 7 27450536
2023 Flot2 acts as a novel mediator of podocyte injury in proteinuric kidney disease. International journal of biological sciences 6 36632460
2023 FLOT2 promotes nasopharyngeal carcinoma progression through suppression of TGF-β pathway via facilitating CD109 expression. iScience 6 38161417
2024 Analysis and Investigation of Bioinformatics and Epigenetics Reveal the Underlying Mechanisms by which FLOT2 Modulates the Progression of Diffuse Large B-cell Lymphoma. Discovery medicine 5 38531803
2020 Complete Genome Sequence of Broad-Host-Range Staphylococcus aureus Myophage ESa1. Microbiology resource announcements 5 32703841
2013 Survivability of immunoassay reagents exposed to the space radiation environment on board the ESA BIOPAN-6 platform as a prelude to performing immunoassays on Mars. Astrobiology 5 23286207
2008 [Expression and significance of CD44(+)ESA(+)CD24(-/low), stem cell markers for breast cancer, in non-small-cell lung carcinoma]. Ai zheng = Aizheng = Chinese journal of cancer 5 18570728
2024 Studies Related to the Involvement of EsA in Improving Intestinal Inflammation in Acute Pancreatitis via the NF-κB Pathway. Mediators of inflammation 4 38590775
2023 Effects of ESA_00986 Gene on Adhesion/Invasion and Virulence of Cronobacter sakazakii and Its Molecular Mechanism. Foods (Basel, Switzerland) 4 37444309
2023 Loss of Flot2 expression in deep cerebellar nuclei neurons of mice with Niemann-Pick disease type C. Heliyon 4 37539272
2023 Knockdown of PAF1 reduces cervical cancer cell proliferation, migration and invasion via retarding FLOT2-mediated MEK/ERK1/2 pathway. Cell adhesion & migration 4 37754347
2022 FLOT2 Promotes the Proliferation and Epithelial-mesenchymal Transition of Cervical Cancer by Activating the MEK/ERK1/2 Pathway. Balkan medical journal 4 35872627
2018 CD44high/ESAlow squamous cell carcinoma cell-derived prostaglandin E2 confers resistance to 5-fluorouracil-induced apoptosis in CD44high/ESAhigh cells. International journal of clinical and experimental pathology 4 31938347
2016 Establishment and characterization of a novel uterine carcinosarcoma cell line, TU-ECS-1, with mutations of TP53 and KRAS. Human cell 4 27889902
2004 Comparing the ESA HPLC total homocysteine assay with electrochemical detection to the CDC in-house HPLC assay with fluorescence detection. Clinica chimica acta; international journal of clinical chemistry 4 14734212
2025 Flot2 protects against podocyte injury by maintaining the stability of synaptopodin in diabetic nephropathy. Metabolism: clinical and experimental 3 40681143
2024 HNRNPH1 stabilizes FLOT2 mRNA in a non-canonical m6A-dependent manner to promote malignant progression in nasopharyngeal carcinoma. Cellular oncology (Dordrecht, Netherlands) 3 39570559
2021 Interaction of replication factor Sld3 and histone acetyl transferase Esa1 alleviates gene silencing and promotes the activation of late and dormant replication origins. Genetics 3 33683348
2025 IPSS-M risk and specific sex-associated somatic mutations predict response to ESA therapy in LR-MDS: building a new score. Blood 2 40561337
2024 Toll-like receptor 4 (TLR4) deficiency impedes Toxoplasma gondii excreted-secreted antigens (ESA)-induced abortion. Placenta 2 38824786