Affinage

FBLIM1

Filamin-binding LIM protein 1 · UniProt Q8WUP2

Length
373 aa
Mass
40.7 kDa
Annotated
2026-04-28
100 papers in source corpus 22 papers cited in narrative 22 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

FBLIM1 (migfilin) is a multidomain focal adhesion scaffold protein that coordinates integrin activation, cytoskeletal organization, cell adhesion, and signal transduction across cell-matrix and cell-cell junctions. Its N-terminal domain competitively displaces filamin from integrin β cytoplasmic tails at a shared binding surface on IgFLNa21, relieving filamin-mediated inhibition and enabling talin-dependent integrin activation, a mechanism critical for platelet αIIbβ3 outside-in signaling and thrombus formation (PMID:19074766, PMID:33131250). The C-terminal LIM domains mediate recruitment to focal adhesions via kindlin-1/2 interaction and to adherens junctions via β-catenin association, while a central proline-rich domain engages VASP to regulate cell migration; migfilin also activates Src kinase to suppress anoikis, modulates ERK1/2-RANKL signaling to control bone remodeling, and shuttles to the nucleus in a calcium-dependent manner to interact with CSX/NKX2-5 and promote cardiomyocyte differentiation (PMID:14757752, PMID:16531412, PMID:15671069, PMID:19833732, PMID:22556421). Recessive FBLIM1 mutations underlie chronic recurrent multifocal osteomyelitis (CRMO), and the cmo mouse model exhibits dramatic downregulation of the Fblim1 ortholog in bone marrow macrophages (PMID:28301468).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 2003 High

    Establishing migfilin as a focal adhesion scaffold resolved how Mig-2/kindlin-2 connects to the actin cytoskeleton via filamin, defining FBLIM1's tripartite architecture and its role in cell shape modulation.

    Evidence Reciprocal Co-IP, pulldown assays, and siRNA knockdown in cultured cells

    PMID:12679033

    Open questions at the time
    • No structural resolution of binding interfaces
    • Redundancy with other LIM-domain scaffolds unknown
    • In vivo requirement not tested
  2. 2004 High

    Discovery that migfilin shuttles to the nucleus in a calcium-dependent manner and interacts with CSX/NKX2-5 to promote cardiomyocyte differentiation revealed a dual cytoplasmic-nuclear function beyond adhesion scaffolding.

    Evidence Yeast two-hybrid, Co-IP, nuclear export signal mapping, calcium-regulated nuclear translocation, and P19CL6 differentiation assay

    PMID:14757752

    Open questions at the time
    • Transcriptional targets of migfilin–NKX2-5 axis uncharacterized
    • In vivo cardiac phenotype not demonstrated
    • Mechanism of calcium-regulated translocation not molecularly defined
  3. 2005 High

    Demonstration that migfilin localizes to adherens junctions via its LIM domains and is required for junction integrity expanded its role from cell-matrix to cell-cell adhesion.

    Evidence Immunoelectron microscopy, siRNA knockdown, domain deletion, and cell-cell adhesion functional assays in epithelial cells

    PMID:15671069

    Open questions at the time
    • Direct binding partner at adherens junctions (beyond β-catenin) not identified
    • Cadherin-type specificity unexplored
  4. 2006 High

    Identification of the migfilin–VASP interaction through a single LPPPPP motif and the EVH1 domain established how migfilin facilitates VASP recruitment to adhesions and promotes cell migration.

    Evidence Co-IP, GST pulldown, point mutagenesis of the LPPPPP motif, siRNA knockdown, and cell migration assays

    PMID:16531412

    Open questions at the time
    • Whether VASP recruitment by migfilin affects actin polymerization dynamics at focal adhesions not directly measured
    • Specificity among EVH1-domain proteins not tested
  5. 2008 High

    Structural determination of the migfilin–filamin interface by crystallography and NMR revealed that migfilin and integrin β tails compete for the same CD-face binding surface on IgFLNa21, providing the atomic basis for migfilin as a molecular switch for integrin activation.

    Evidence X-ray crystallography, NMR spectroscopy, and competitive binding assays with purified proteins

    PMID:18829455 PMID:19074766

    Open questions at the time
    • How the switch is spatiotemporally regulated inside cells not resolved
    • Contribution of mechanical force to filamin opening not addressed
  6. 2008 Medium

    Showing that kindlin-1 and kindlin-2 both bind migfilin yet that kindlin-1 loss does not affect migfilin localization indicated functional redundancy among kindlins in migfilin recruitment.

    Evidence Co-IP, confocal microscopy, and siRNA knockdown in keratinocytes

    PMID:18528435

    Open questions at the time
    • Triple kindlin depletion not performed
    • Whether kindlin-2 alone is sufficient in all cell types not tested
  7. 2009 High

    The finding that migfilin directly binds and activates Src kinase to suppress anoikis linked focal adhesion signaling to a cell survival pathway, broadening FBLIM1's role beyond structural scaffolding.

    Evidence Reciprocal Co-IP, direct binding assay, Src kinase activity assay, caspase activation upon migfilin depletion

    PMID:19833732

    Open questions at the time
    • Structural basis of Src activation by migfilin not resolved
    • Whether Src activation is direct or requires co-factors unknown
  8. 2011 Medium

    Migfilin knockout mice developed normally with intact integrin activation, revealing substantial in vivo redundancy, though keratinocyte migration was mildly impaired, implying tissue-specific requirements.

    Evidence Migfilin-null mouse generation, integrin activation assays, wound scratch migration assays

    PMID:21224394

    Open questions at the time
    • Identity of compensatory gene(s) not determined
    • Bone and platelet phenotypes not yet examined in this study
  9. 2012 High

    FBLIM1 knockout mice exhibited osteopenia with increased osteoclastogenesis driven by ERK1/2-mediated RANKL upregulation, establishing migfilin as a negative regulator of bone resorption signaling.

    Evidence Genetic knockout mouse, bone histomorphometry, BMSC cultures, ERK inhibitor rescue of RANKL levels

    PMID:22556421

    Open questions at the time
    • Molecular mechanism linking migfilin to ERK1/2 activation not defined
    • Whether the bone phenotype is cell-autonomous to osteoblasts versus osteoclasts not fully resolved
  10. 2013 High

    FRAP and FRET analyses demonstrated that kindlin binding governs migfilin focal adhesion dynamics, and that without the LIM domains the N-terminal region directs migfilin to stress fibers, establishing a modular targeting code.

    Evidence Kindlin knockdown, FRET/FRAP, domain deletions in cultured cells

    PMID:24165133

    Open questions at the time
    • Whether force-dependent changes in kindlin modulate migfilin residence time not tested
    • Stoichiometry of migfilin within adhesion complexes unknown
  11. 2015 Medium

    A Src–kindlin-2–migfilin positive feedback loop was identified in which Src phosphorylation of kindlin-2 Y193 enhances migfilin binding and focal adhesion recruitment, providing the first phospho-regulatory mechanism for the migfilin–kindlin interaction.

    Evidence In vitro kinase assay, phospho-mutant analysis, Co-IP, cell spreading and migration assays

    PMID:26037143

    Open questions at the time
    • In vivo significance of Y193 phosphorylation not tested
    • Whether other kinases can substitute for Src not examined
  12. 2017 Medium

    Identification of recessive FBLIM1 mutations in CRMO patients, coupled with dramatic Fblim1 downregulation in the cmo mouse, established a Mendelian disease link and connected loss of migfilin to sterile bone inflammation.

    Evidence Whole-exome sequencing, enhancer reporter assay, microarray expression profiling in cmo mouse bone marrow macrophages

    PMID:28301468

    Open questions at the time
    • Causal rescue of CRMO phenotype by FBLIM1 re-expression not demonstrated
    • Penetrance and genetic heterogeneity in CRMO families not fully defined
    • Single study without independent replication
  13. 2020 High

    Migfilin-null mice displayed prolonged bleeding and defective thrombus formation due to impaired αIIbβ3 outside-in signaling; a cell-permeable migfilin peptide rescued the defect by preventing filamin A–β3 re-association, providing direct in vivo proof that the filamin-displacement mechanism operates in hemostasis.

    Evidence Migfilin KO mice, tail-bleeding assay, FeCl3 thrombosis model, platelet aggregation and dense-granule secretion assays, cell-permeable peptide rescue, Co-IP

    PMID:33131250

    Open questions at the time
    • Whether migfilin peptide has therapeutic potential in thrombotic disease not addressed
    • Role of migfilin in other platelet integrins not examined

Open questions

Synthesis pass · forward-looking unresolved questions
  • The molecular basis by which migfilin toggles between focal adhesion, adherens junction, and nuclear functions — and how these distinct pools are coordinated in vivo — remains unresolved.
  • No structural model of full-length migfilin
  • Post-translational modification landscape beyond Y193 on kindlin-2 largely unexplored
  • Compensatory genes masking KO phenotypes not identified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 4 GO:0060090 molecular adaptor activity 4 GO:0098772 molecular function regulator activity 4
Localization
GO:0005886 plasma membrane 4 GO:0005856 cytoskeleton 3 GO:0005829 cytosol 2 GO:0005634 nucleus 1
Pathway
R-HSA-1500931 Cell-Cell communication 3 R-HSA-162582 Signal Transduction 3 R-HSA-1474244 Extracellular matrix organization 2 R-HSA-109582 Hemostasis 1 R-HSA-5357801 Programmed Cell Death 1
Partners
FERMT1FERMT2FLNAFLNBFLNCNKX2-5SRCVASP
Complex memberships
Adherens junction complexIntegrin-kindlin-migfilin-filamin complex

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 Migfilin (FBLIM1) localizes to cell-matrix adhesions, interacts with Mig-2 (kindlin-2 homolog UNC-112) through its C-terminal domain and with filamin through its N-terminal domain, thereby functioning as a scaffold linking cell-matrix adhesions to the actin cytoskeleton and regulating cell shape modulation. Co-immunoprecipitation, pulldown assays, siRNA knockdown, loss-of-function phenotypic analysis (cell shape modulation defects) Cell High 12679033
2008 The N-terminal portion of migfilin binds all three human filamins (FLNa, FLNb, FLNc), preferentially to IgFLNa21 and more weakly to IgFLNa19 and IgFLNa22 via the CD face of the IgFLN beta-sandwich. The migfilin-filamin binding site (Pro5–Pro19) overlaps with the integrin beta tail binding site on IgFLNa21, allowing migfilin and integrin beta tails to compete for the same filamin binding surface. X-ray crystallography, NMR spectroscopy, protein-protein interaction competition assays The Journal of biological chemistry High 18829455
2008 Migfilin structurally interacts with filamin at the same region where integrin beta cytoplasmic tails bind, and this interaction dissociates filamin from integrin, promoting talin/integrin binding and integrin activation; migfilin thus acts as a molecular switch between filamin-mediated inhibition and talin-mediated activation of integrins. NMR structural analysis, pulldown assays, integrin activation functional assays The Journal of biological chemistry High 19074766
2005 Migfilin contains an N-terminal filamin-binding domain, a central proline-rich domain, and three C-terminal LIM domains; it is recruited to cell-matrix contacts upon adhesion, colocalizes with beta-catenin at cell-cell junctions, shuttles from cytoplasm to nucleus in a process regulated by RNA splicing and calcium signaling, and interacts with VASP and the cardiac transcription factor CSX/NKX2-5. Biochemical interaction studies, live-cell imaging, domain deletion analysis, yeast two-hybrid Journal of cell science Medium 15701922
2006 Migfilin interacts with vasodilator-stimulated phosphoprotein (VASP) via the VASP EVH1 domain and a single LPPPPP site in the migfilin proline-rich domain; this interaction facilitates VASP localization to cell-matrix adhesions and is required for migfilin-regulated cell migration. Co-immunoprecipitation, GST pulldown, domain mutagenesis, siRNA knockdown, cell migration assays The Journal of biological chemistry High 16531412
2004 Migfilin (Cal/FBLIM1) interacts with the cardiac transcription factor CSX/NKX2-5 via its LIM domains and the NKX2-5 homeodomain; migfilin possesses transcription-promoting activity, contains a functional nuclear export signal, shuttles to the nucleus in response to calcium, and promotes myocardial cell differentiation when accumulated in the nucleus. Yeast two-hybrid, Co-IP, in vitro binding, nuclear export signal analysis, calcium-regulated nuclear translocation assay, P19CL6 differentiation assay The Journal of cell biology High 14757752
2005 Migfilin is recruited to cell-cell junctions (adherens junctions) in response to cadherin-mediated adhesion; the C-terminal LIM domains mediate this localization; migfilin associates with beta-catenin but not desmosomes at cell-cell junctions; siRNA-mediated depletion of migfilin compromises adherens junction organization and weakens cell-cell association. Immunofluorescence, immunoelectron microscopy, siRNA knockdown, domain deletion analysis, cell-cell adhesion assay Journal of cell science High 15671069
2009 Migfilin directly interacts with Src kinase, with the migfilin binding surface overlapping the inhibitory intramolecular interaction sites in Src; this interaction activates Src, leading to suppression of anoikis; loss of cell-ECM adhesion reduces migfilin levels and induces apoptosis, and overexpression of migfilin desensitizes cells to detachment-induced apoptosis. Co-immunoprecipitation, direct binding assays, overexpression/depletion experiments, apoptosis assays (caspase activation), Src kinase activity assays The Journal of biological chemistry High 19833732
2011 Migfilin displaces filamin from beta1 and beta3 integrins and promotes integrin activation in endothelial cells and neutrophils; filamin acts broadly as an inhibitor of integrin activation and migfilin overcomes this inhibition; migfilin depletion impairs spreading and migration of endothelial cells. Flow cytometry (integrin activation), siRNA knockdown, spreading and migration assays in primary vascular cells PloS one Medium 22043318
2011 NMR spectroscopy demonstrates that filamin autoinhibition of integrin-binding repeats 19 and 21 can be relieved by integrin or by migfilin via a multisite binding mechanism, with repeats 19 and 21 simultaneously engaging ligands, suggesting filamin is mechanically stretched by migfilin/integrin interaction. NMR spectroscopy, binding assays Biochemistry Medium 21524097
2008 Kindlin-1 and kindlin-2 interact with migfilin (FBLIM1) and colocalize at focal adhesions in keratinocytes; loss of kindlin-1 does not affect FBLIM1 gene expression or migfilin protein localization, indicating migfilin can function independently of kindlin-1. Co-immunoprecipitation, confocal microscopy, siRNA knockdown, immunostaining The Journal of investigative dermatology Medium 18528435
2012 Genetic inactivation of FBLIM1 (encoding FBLP-1/migfilin) in mice causes osteopenic phenotype; loss of FBLP-1 impairs BMSC adhesion, migration, growth, and survival; reduces osteoblast progenitors and differentiation; dramatically increases osteoclast differentiation; markedly elevates RANKL in null BMSCs via ERK1/2 hyperactivation; ERK1/2 inhibition suppresses the RANKL increase. Genetic knockout (mouse), primary cell culture assays (adhesion, migration, proliferation, differentiation), in vivo bone histomorphometry, western blot (RANKL, pERK), pharmacological ERK inhibition The Journal of biological chemistry High 22556421
2012 Migfilin promotes migration and invasion in glioma cells by positively modulating EGFR expression and activity; migfilin-mediated migration and invasion depend on EGFR-induced PLC-gamma and STAT3 signaling pathways. Immunohistochemistry, siRNA knockdown, migration/invasion assays, western blot (EGFR, PLC-gamma, STAT3 phosphorylation) The Journal of biological chemistry Medium 22843679
2012 Migfilin inhibits esophageal cancer cell motility by promoting GSK-3beta-mediated phosphorylation and proteasomal degradation of beta-catenin; migfilin reinforces the association between beta-catenin and GSK-3beta; overexpression of beta-catenin or inhibition of GSK-3beta reverses migfilin-mediated suppression of invasion. Overexpression, siRNA knockdown, beta-catenin reporter assay, co-IP (migfilin–beta-catenin–GSK-3beta complex), proteasome inhibitor rescue, invasion assay Molecular cancer research : MCR Medium 22246236
2013 Kindlin-1 and kindlin-2 bind the C-terminal tandem LIM domains of migfilin; this interaction drives migfilin FA recruitment, localization, and mobility; FRAP and FRET analyses show kindlin depletion alters migfilin dynamics; when the C-terminal LIM region is deleted, the N-terminal filamin-binding region directs migfilin to actin stress fibers instead. Kindlin knockdown, biochemical pulldown, fluorescence microscopy, FRET, FRAP, domain deletion analysis The Journal of biological chemistry High 24165133
2015 Src phosphorylates kindlin-2 at Y193, which enhances Src kinase activity; kindlin-2 Y193 phosphorylation is required for migfilin binding to kindlin-2 and the recruitment of migfilin to focal adhesions; this constitutes a positive feedback loop among Src, kindlin-2, and migfilin. In vitro kinase assay, phospho-specific mutant analysis, Co-IP, immunofluorescence, cell spreading and migration assays FEBS letters Medium 26037143
2017 Recessive mutations in the filamin-binding domain of FBLIM1, as well as regulatory variants ablating an enhancer, underlie chronic recurrent multifocal osteomyelitis (CRMO); the Fblim1 ortholog is the most differentially expressed gene (>20-fold downregulated) in bone marrow macrophages of the cmo mouse model. Whole-exome sequencing, Sanger sequencing, microarray expression analysis of cmo mouse BMMs, enhancer reporter assay in SaOS2 cells PloS one Medium 28301468
2018 FBLIM1 knockdown in OSCC cells attenuates proliferation, migration, and invasiveness; FBLIM1 expression correlates with EGFR activity; clopidogrel treatment downregulates both EGFR and FBLIM1, phenocopying the KD effect, placing FBLIM1 upstream or co-regulatory with EGFR signaling in oral cancer. siRNA knockdown, clopidogrel pharmacological inhibition, qRT-PCR, immunoblot, invasion/migration assays, immunohistochemistry in primary OSCC specimens Molecular carcinogenesis Medium 30129678
2020 Migfilin is a pivotal positive regulator of platelet αIIbβ3 outside-in signaling; migfilin-null mice show doubled tail-bleeding time and prolonged thrombosis; migfilin-/- platelets have defective thrombus formation, aggregation, and dense-granule secretion; mechanistically, migfilin hampers re-association of filamin A with the beta3 subunit of αIIbβ3 during outside-in signaling; a cell-permeable migfilin peptide harboring the filamin A-binding sequence rescues the defective function. Migfilin knockout mice, tail-bleeding assay, FeCl3-induced thrombosis model, platelet function assays, phosphorylation analysis of signaling molecules, cell-permeable peptide rescue, Co-IP (filamin A–beta3 interaction) Haematologica High 33131250
2011 Loss of migfilin expression in mice permits normal development, postnatal aging, and normal integrin expression and activation in fibroblasts and keratinocytes; migration velocity of keratinocytes was slightly but significantly reduced, suggesting functional redundancy in vivo. Migfilin-null mouse generation, developmental analysis, cell spreading/adhesion assays, integrin activation assays, wound scratch migration assay Journal of cell science Medium 21224394
2015 Migfilin silencing in HCC (HepG2) cells upregulates phospho-VASP (Ser157, Ser239), Fascin-1, and Rho-kinase-1, promoting actin polymerization; decreases phospho-Akt, upregulates free and phospho-beta-catenin, induces proliferation; and upregulates ERK, increasing cell adhesion while reducing invasiveness. siRNA knockdown, western blot (signaling pathway analysis), invasion assay, cell adhesion assay Experimental cell research Medium 25773778
2017 VASP silencing in MDA-MB-231 breast cancer cells downregulates migfilin, beta-catenin, and urokinase-plasminogen activator (uPA) in 2D and 3D culture, and impairs tumor spheroid invasion; placing migfilin downstream of VASP in invasion-related signaling. siRNA knockdown of VASP, 3D collagen gel invasion assays, western blot, atomic force microscopy Experimental cell research Medium 28209486

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2010 CAL-101, a p110delta selective phosphatidylinositol-3-kinase inhibitor for the treatment of B-cell malignancies, inhibits PI3K signaling and cellular viability. Blood 617 20959606
2010 Phosphatidylinositol 3-kinase-δ inhibitor CAL-101 shows promising preclinical activity in chronic lymphocytic leukemia by antagonizing intrinsic and extrinsic cellular survival signals. Blood 459 20522708
2011 The phosphoinositide 3'-kinase delta inhibitor, CAL-101, inhibits B-cell receptor signaling and chemokine networks in chronic lymphocytic leukemia. Blood 454 21803855
2003 Migfilin and Mig-2 link focal adhesions to filamin and the actin cytoskeleton and function in cell shape modulation. Cell 309 12679033
2011 PI3Kδ inhibitor, GS-1101 (CAL-101), attenuates pathway signaling, induces apoptosis, and overcomes signals from the microenvironment in cellular models of Hodgkin lymphoma. Blood 127 22210877
2021 CRISPR/Cas12a-Assisted Ligation-Initiated Loop-Mediated Isothermal Amplification (CAL-LAMP) for Highly Specific Detection of microRNAs. Analytical chemistry 125 34038095
2003 Modulation of mature cystic fibrosis transmembrane regulator protein by the PDZ domain protein CAL. The Journal of biological chemistry 109 14570915
2008 Structural basis of the migfilin-filamin interaction and competition with integrin beta tails. The Journal of biological chemistry 96 18829455
2008 Migfilin, a molecular switch in regulation of integrin activation. The Journal of biological chemistry 94 19074766
2018 Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy. International journal of nanomedicine 91 29950828
2007 SHP2-mediated signaling cascade through gp130 is essential for LIF-dependent I CaL, [Ca2+]i transient, and APD increase in cardiomyocytes. Journal of molecular and cellular cardiology 88 17961593
2011 Inhibitory effect of curcumin on oral carcinoma CAL-27 cells via suppression of Notch-1 and NF-κB signaling pathways. Journal of cellular biochemistry 85 21308734
2008 Colocalization of kindlin-1, kindlin-2, and migfilin at keratinocyte focal adhesion and relevance to the pathophysiology of Kindler syndrome. The Journal of investigative dermatology 67 18528435
2011 CAL-101: a phosphatidylinositol-3-kinase p110-delta inhibitor for the treatment of lymphoid malignancies. Expert opinion on investigational drugs 64 22112004
2017 Recessive coding and regulatory mutations in FBLIM1 underlie the pathogenesis of chronic recurrent multifocal osteomyelitis (CRMO). PloS one 63 28301468
2007 Identification of a distinct side population of cancer cells in the Cal-51 human breast carcinoma cell line. Molecular and cellular biochemistry 63 17660947
2005 Migfilin and its binding partners: from cell biology to human diseases. Journal of cell science 63 15701922
2011 Migfilin and filamin as regulators of integrin activation in endothelial cells and neutrophils. PloS one 59 22043318
2004 Interaction with cystic fibrosis transmembrane conductance regulator-associated ligand (CAL) inhibits beta1-adrenergic receptor surface expression. The Journal of biological chemistry 57 15358775
2010 Syntaxin 6 and CAL mediate the degradation of the cystic fibrosis transmembrane conductance regulator. Molecular biology of the cell 56 20130090
2006 Migfilin interacts with vasodilator-stimulated phosphoprotein (VASP) and regulates VASP localization to cell-matrix adhesions and migration. The Journal of biological chemistry 54 16531412
2004 A novel LIM protein Cal promotes cardiac differentiation by association with CSX/NKX2-5. The Journal of cell biology 52 14757752
2017 The main rhinovirus respiratory tract adhesion site (ICAM-1) is upregulated in smokers and patients with chronic airflow limitation (CAL). Respiratory research 51 28056984
2012 Critical role of filamin-binding LIM protein 1 (FBLP-1)/migfilin in regulation of bone remodeling. The Journal of biological chemistry 50 22556421
2013 Ubiquitination and degradation of CFTR by the E3 ubiquitin ligase MARCH2 through its association with adaptor proteins CAL and STX6. PloS one 49 23818989
2016 Multilineage polyclonal engraftment of Cal-1 gene-modified cells and in vivo selection after SHIV infection in a nonhuman primate model of AIDS. Molecular therapy. Methods & clinical development 44 26958575
2005 Physical and functional association of migfilin with cell-cell adhesions. Journal of cell science 40 15671069
2014 AMPK-dependent signaling modulates the suppression of invasion and migration by fenofibrate in CAL 27 oral cancer cells through NF-κB pathway. Environmental toxicology 39 25545733
2009 CAL 27 is an oral adenosquamous carcinoma cell line. Oral oncology 39 19631575
2018 Positive Feedback Mechanisms among Local Ca Releases, NCX, and ICaL Ignite Pacemaker Action Potentials. Biophysical journal 38 29539403
2019 Ginsenoside Rb1 exerts antiarrhythmic effects by inhibiting INa and ICaL in rabbit ventricular myocytes. Scientific reports 36 31892729
2015 Anthocyanins from black rice (Oryza sativa L.) demonstrate antimetastatic properties by reducing MMPs and NF-κB expressions in human oral cancer CAL 27 cells. Nutrition and cancer 35 25658905
2014 Cell death caused by quinazolinone HMJ-38 challenge in oral carcinoma CAL 27 cells: dissections of endoplasmic reticulum stress, mitochondrial dysfunction and tumor xenografts. Biochimica et biophysica acta 33 24594224
2011 Proteomic analysis of anti-tumor effects of 11-dehydrosinulariolide on CAL-27 cells. Marine drugs 33 21822415
2022 Tagging active neurons by soma-targeted Cal-Light. Nature communications 32 36509775
2020 Effects of mesalazine combined with bifid triple viable on intestinal flora, immunoglobulin and levels of cal, MMP-9, and MPO in feces of patients with ulcerative colitis. European review for medical and pharmacological sciences 32 32017001
2019 Liposome-coated nano doxorubicin induces apoptosis on oral squamous cell carcinoma CAL-27 cells. Archives of oral biology 32 31132617
2011 Loss of migfilin expression has no overt consequences on murine development and homeostasis. Journal of cell science 32 21224394
2009 Migfilin interacts with Src and contributes to cell-matrix adhesion-mediated survival signaling. The Journal of biological chemistry 32 19833732
2012 A lactose-binding lectin from the marine sponge Cinachyrella apion (Cal) induces cell death in human cervical adenocarcinoma cells. Marine drugs 31 22690140
2012 Migfilin protein promotes migration and invasion in human glioma through epidermal growth factor receptor-mediated phospholipase C-γ and STAT3 protein signaling pathways. The Journal of biological chemistry 31 22843679
2004 Group B rotaviruses similar to strain CAL-1, have been circulating in Western India since 1993. Epidemiology and infection 31 15310177
1998 Replication Specificity Elements of the Worland Strain of Beet Curly Top Virus Are Compatible with Those of the CFH Strain But Not Those of the Cal/Logan Strain. Phytopathology 29 18944850
2017 Vasodilator-Stimulated Phosphoprotein (VASP) depletion from breast cancer MDA-MB-231 cells inhibits tumor spheroid invasion through downregulation of Migfilin, β-catenin and urokinase-plasminogen activator (uPA). Experimental cell research 28 28209486
2009 The PDZ domain protein CAL interacts with mGluR5a and modulates receptor expression. Journal of neurochemistry 28 19860857
2012 AKT serine/threonine protein kinase modulates bufalin-triggered intrinsic pathway of apoptosis in CAL 27 human oral cancer cells. International journal of oncology 27 22922805
2012 Migfilin regulates esophageal cancer cell motility through promoting GSK-3β-mediated degradation of β-catenin. Molecular cancer research : MCR 26 22246236
2008 A novel association of mGluR1a with the PDZ scaffold protein CAL modulates receptor activity. FEBS letters 26 19027007
2021 Cal'MAM'ity at the Endoplasmic Reticulum-Mitochondrial Interface: A Potential Therapeutic Target for Neurodegeneration and Human Immunodeficiency Virus-Associated Neurocognitive Disorders. Frontiers in neuroscience 25 34744606
2020 Synergistic Effect of Dietary Betaines on SIRT1-Mediated Apoptosis in Human Oral Squamous Cell Carcinoma Cal 27. Cancers 25 32878301
2022 Integrated Genomic and Metabolomic Analysis Illuminates Key Secreted Metabolites Produced by the Novel Endophyte Bacillus halotolerans Cal.l.30 Involved in Diverse Biological Control Activities. Microorganisms 24 35208854
2011 Prevalence of Rickettsia felis and the first identification of Bartonella henselae Fizz/CAL-1 in cat fleas (Siphonaptera: Pulicidae) from Taiwan. Journal of medical entomology 24 21485388
2011 Evidence for multisite ligand binding and stretching of filamin by integrin and migfilin. Biochemistry 24 21524097
2007 Increased cytoplasmic level of migfilin is associated with higher grades of human leiomyosarcoma. Histopathology 22 17711449
2021 Identification of cell lines CL-14, CL-40 and CAL-51 as suitable models for SARS-CoV-2 infection studies. PloS one 21 34339474
2015 Kindlin-2 phosphorylation by Src at Y193 enhances Src activity and is involved in Migfilin recruitment to the focal adhesions. FEBS letters 21 26037143
2014 Paleopathological description and diagnosis of metastatic carcinoma in an Early Bronze Age (4588+34 Cal. BP) forager from the Cis-Baikal region of Eastern Siberia. PloS one 21 25470373
2013 Spindle and kinetochore associated complex subunit 1 regulates the proliferation of oral adenosquamous carcinoma CAL-27 cells in vitro. Cancer cell international 21 23962337
2017 PI3K-δ inhibition using CAL-101 exerts apoptotic effects and increases doxorubicin-induced cell death in pre-B-acute lymphoblastic leukemia cells. Anti-cancer drugs 20 28125433
2012 The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans-fatty acid selectivity. Applied microbiology and biotechnology 20 22294433
2013 Kindlin binds migfilin tandem LIM domains and regulates migfilin focal adhesion localization and recruitment dynamics. The Journal of biological chemistry 19 24165133
2020 PITX2 upregulation increases the risk of chronic atrial fibrillation in a dose-dependent manner by modulating I and I -insights from human atrial modelling. Annals of translational medicine 18 32309338
2020 Cyclic Peptidyl Inhibitors against CAL/CFTR Interaction for Treatment of Cystic Fibrosis. Journal of medicinal chemistry 18 33314931
2017 In vivo deep two-photon imaging of neural circuits with the fluorescent Ca2+ indicator Cal-590. The Journal of physiology 18 27995645
2016 Lipid nanoemulsions and liposomes improve photodynamic treatment efficacy and tolerance in CAL-33 tumor bearing nude mice. Journal of nanobiotechnology 18 27716314
2016 Rationale behind the near-ideal catalysis of Candida antarctica lipase A (CAL-A) for highly concentrating ω-3 polyunsaturated fatty acids into monoacylglycerols. Food chemistry 18 27765222
2015 The 3D model of the lipase/acyltransferase from Candida parapsilosis, a tool for the elucidation of structural determinants in CAL-A lipase superfamily. Biochimica et biophysica acta 18 26123263
2010 Kinetic resolution of (R/S)-propranolol (1-isopropylamino-3-(1-naphtoxy)-2-propanolol) catalyzed by immobilized preparations of Candida antarctica lipase B (CAL-B). New biotechnology 18 20667519
2022 V1-Cal hydrogelation enhances its effects on ventricular remodeling reduction and cardiac function improvement post myocardial infarction. Chemical engineering journal (Lausanne, Switzerland : 1996) 17 36338580
2019 Dynamic clamping human and rabbit atrial calcium current: narrowing ICaL window abolishes early afterdepolarizations. The Journal of physiology 17 31093979
2020 Genetic variants in FBLIM1 gene do not contribute to SAPHO syndrome and chronic recurrent multifocal osteomyelitis in typical patient groups. BMC medical genetics 16 32397996
2019 Optically Induced Calcium-Dependent Gene Activation and Labeling of Active Neurons Using CaMPARI and Cal-Light. Frontiers in synaptic neuroscience 16 31178713
2017 Metagene projection characterizes GEN2.2 and CAL-1 as relevant human plasmacytoid dendritic cell models. Bioinformatics (Oxford, England) 16 28961902
2015 Experimental evidence of Migfilin as a new therapeutic target of hepatocellular carcinoma metastasis. Experimental cell research 16 25773778
2015 IRF5 and IRF8 modulate the CAL-1 human plasmacytoid dendritic cell line response following TLR9 ligation. European journal of immunology 16 26613957
2014 Total flavonoids from Ganshanbian (Herba Hyperici Attenuati) effect the expression of CaL-alpha1C and K(ATP)-Kir6.1 mRNA of the myocardial cell membrane in myocardial ischemia-reperfusion arrhythmia rats. Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 16 24992765
2001 A comparison of CAL with a conventional method of delivery of cell biology to undergraduate nursing students using an experimental design. Nurse education today 16 11559012
2018 FBLIM1 enhances oral cancer malignancy via modulation of the epidermal growth factor receptor pathway. Molecular carcinogenesis 15 30129678
2015 Elucidation of a key position for acyltransfer activity in Candida parapsilosis lipase/acyltransferase (CpLIP2) and in Pseudozyma antarctica lipase A (CAL-A) by rational design. Biochimica et biophysica acta 15 26602447
2023 Antitumor Activity of Berberine by Activating Autophagy and Apoptosis in CAL-62 and BHT-101 Anaplastic Thyroid Carcinoma Cell Lines. Drug design, development and therapy 14 37397788
2019 PDZ Scaffold Protein CAL Couples with Metabotropic Glutamate Receptor 5 to Protect Against Cell Apoptosis and Is a Potential Target in the Treatment of Parkinson's Disease. Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 14 31073978
2019 Inhibition of Cancer Stem-Like Phenotype by Curcumin and Deguelin in CAL-62 Anaplastic Thyroid Cancer Cells. Anti-cancer agents in medicinal chemistry 14 31584382
2011 Crude extracts of Agaricus brasiliensis induce apoptosis in human oral cancer CAL 27 cells through a mitochondria-dependent pathway. In vivo (Athens, Greece) 14 21576408
2019 Synergistic Effects of PI3K and c-Myc Co-targeting in Acute Leukemia: Shedding New Light on Resistance to Selective PI3K-δ Inhibitor CAL-101. Cancer investigation 13 31412710
2015 A genome-wide study of DNA methylation modified by epigallocatechin-3-gallate in the CAL-27 cell line. Molecular medicine reports 13 26239270
2015 ROCK inhibitor Y-27632 inhibits the growth, migration, and invasion of Tca8113 and CAL-27 cells in tongue squamous cell carcinoma. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 13 26468018
2022 Osmanthus-Loaded PVP/PVA Hydrogel Inhibits the Proliferation and Migration of Oral Squamous Cell Carcinoma Cells CAL-27. Polymers 12 36559766
2016 Karyopherin α2 induces apoptosis in tongue squamous cell carcinoma CAL-27 cells through the p53 pathway. Oncology reports 12 27109484
2011 Crystallization and preliminary diffraction analysis of the CAL PDZ domain in complex with a selective peptide inhibitor. Acta crystallographica. Section F, Structural biology and crystallization communications 12 21543871
1975 Isolation and characterization of cal allergens. Clinical allergy 12 810266
2023 Compatible Consortium of Endophytic Bacillus halotolerans Strains Cal.l.30 and Cal.f.4 Promotes Plant Growth and Induces Systemic Resistance against Botrytis cinerea. Biology 11 37372064
2020 High prevalence of rare FBLIM1 gene variants in an Italian cohort of patients with Chronic Non-bacterial Osteomyelitis (CNO). Pediatric rheumatology online journal 11 32650789
2018 Cysteine modifiers suggest an allosteric inhibitory site on the CAL PDZ domain. Bioscience reports 11 29472314
2017 Wenxin Keli diminishes Ca2+ overload induced by hypoxia/reoxygenation in cardiomyocytes through inhibiting INaL and ICaL. Pacing and clinical electrophysiology : PACE 11 28972668
2016 The immunomodulatory activities of pullulan and its derivatives in human pDC-like CAL-1 cell line. International journal of biological macromolecules 11 26854885
2013 Mitogen-inducible Gene-2 (MIG2) and migfilin expression is reduced in samples of human breast cancer. Anticancer research 11 23645746
2012 In vitro effect of molluscan hemocyanins on CAL-29 and T-24 bladder cancer cell lines. Biomedical reports 11 24648926
2009 Nm23-H1 promotes adhesion of CAL 27 cells in vitro. Molecular carcinogenesis 11 19263457
1991 Brief exposure to the G-protein activator NaF/AlCl3 induces prolonged enhancement of synaptic transmission in area CAl of rat hippocampal slices. Experimental brain research 11 1650708
2020 Migfilin supports hemostasis and thrombosis through regulating platelet αIIbβ3 outside-in signaling. Haematologica 10 33131250