Affinage

EXOC7

Exocyst complex component 7 · UniProt Q9UPT5

Length
735 aa
Mass
83.4 kDa
Annotated
2026-04-28
72 papers in source corpus 34 papers cited in narrative 34 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

EXOC7 (Exo70) is a core subunit of the octameric exocyst complex that tethers post-Golgi secretory vesicles to the plasma membrane, coupling polarized exocytosis with actin-based membrane remodeling and cell migration. Exo70 anchors the exocyst at the plasma membrane through direct binding to PI(4,5)P2 via its C-terminal Domain D and through interaction with polarity scaffolds such as Bem1p, and serves as a spatial landmark to which vesicle-borne exocyst subunits are recruited for complex assembly (PMID:17717527, PMID:15583031, PMID:25313406). Independent of vesicle tethering, Exo70 directly activates the Arp2/3 complex—potentiated by WAVE2—to drive actin-dependent lamellipodia and membrane protrusions, with ESRP1-regulated isoform switching determining whether the epithelial or mesenchymal splice variant engages Arp2/3 to control invasive behavior (PMID:17086175, PMID:22748316, PMID:24331928). Exocyst assembly and Exo70 function are positively regulated by ERK1/2 phosphorylation and TGM1/3-mediated transamidation, and negatively regulated by ULK1 phosphorylation that inhibits Exo70 oligomerization; Rho-family GTPases (Rho3, TC10, Cdc42b) control Exo70 membrane recruitment in a GTP-dependent manner to direct exocytosis in contexts ranging from GLUT4 trafficking in adipocytes to axon specification in neurons (PMID:22595671, PMID:31913283, PMID:39146185, PMID:10207081, PMID:31740584, PMID:19846717). Partial loss-of-function variants in EXOC7 cause a recessive neurodevelopmental disorder with cerebral cortex atrophy, seizures, and microcephaly (PMID:32103185).

Mechanistic history

Synthesis pass · year-by-year structured walk · 20 steps
  1. 1999 High

    Identifying Exo70 as a GTP-dependent effector of the Rho3 GTPase established the first direct link between Rho-family signaling and the exocyst, raising the question of how GTPase binding regulates exocyst targeting.

    Evidence Yeast two-hybrid and purified protein binding assay with immunofluorescence colocalization in yeast

    PMID:10207081

    Open questions at the time
    • Structural basis of Rho3–Exo70 interaction unknown at this stage
    • Whether interaction is conserved in metazoans not tested
  2. 2004 High

    Demonstrating that Exo70 (with Sec3) is a stable plasma membrane landmark rather than a vesicle-borne component resolved a fundamental question about exocyst architecture: complex assembly occurs when vesicle subunits dock onto membrane-resident Exo70/Sec3.

    Evidence FRAP, immunogold EM, and live imaging of exocyst subunit dynamics in yeast

    PMID:15583031

    Open questions at the time
    • Molecular basis of Exo70 membrane association not yet defined
    • Whether this split architecture applies in mammalian cells unclear
  3. 2005 High

    Solving the crystal structure of Exo70 revealed a novel elongated all-α-helical rod fold and provided quantitative binding data for Rho3 (Kd ~70 µM), establishing the structural framework for understanding how Exo70 bridges GTPase signaling and membrane tethering.

    Evidence X-ray crystallography at 2.0 Å and 3.5 Å resolution with equilibrium binding assays (two independent studies)

    PMID:16249794 PMID:16359701

    Open questions at the time
    • Structure of Exo70 in complex with Rho3 or other exocyst subunits not determined
    • Functional significance of individual helical domains unclear
  4. 2006 High

    Discovery that Exo70 directly interacts with the Arp2/3 complex and is required for actin-based membrane protrusions revealed an exocyst-independent function in cytoskeletal remodeling, broadening Exo70's role beyond vesicle tethering.

    Evidence Co-immunoprecipitation, RNAi, antibody microinjection, and live cell imaging in mammalian cells

    PMID:17086175

    Open questions at the time
    • Mechanism by which Exo70 activates Arp2/3 not determined
    • Whether vesicle tethering and Arp2/3 activation are coordinated unknown
  5. 2007 High

    Identifying PI(4,5)P2 binding by Exo70's Domain D as the essential determinant of plasma membrane association (superseding Rho3 binding) resolved how Exo70 achieves membrane anchoring and established a lipid-dependent targeting mechanism for exocyst function.

    Evidence Direct lipid-binding assays, key residue mutagenesis, and genetic epistasis in yeast

    PMID:17717527

    Open questions at the time
    • Whether PI(4,5)P2 binding is sufficient in mammalian cells or requires co-receptors not tested
    • Relationship between PI(4,5)P2 and Rho-family GTPase binding not fully dissected
  6. 2007 High

    Showing that Exo70 selectively mediates tethering of Bgl2p-class vesicles during early cell cycle stages demonstrated cargo selectivity in exocyst function, indicating the complex does not act as a universal tether for all post-Golgi vesicles.

    Evidence Genetic analysis of exo70 mutants with cargo-specific secretion assays in yeast

    PMID:17339375

    Open questions at the time
    • Molecular determinants of cargo selectivity unknown
    • Whether selectivity extends to mammalian cargo classes untested
  7. 2007 Medium

    FRET-FLIM demonstration that NGF-activated TC10 interacts with Exo70 and locally antagonizes Cdc42/N-WASP revealed that Exo70-GTPase interactions shape the mode of membrane outgrowth during neurite formation by coordinating distinct Rho-family outputs.

    Evidence FRET-FLIM, dominant-negative mutants, siRNA knockdown, and N-WASP biosensor in PC12 cells

    PMID:17635999

    Open questions at the time
    • Whether TC10–Exo70 antagonism of N-WASP occurs in all neuronal types not established
    • Structural basis of TC10–Exo70 interaction unknown
  8. 2008 High

    Domain C dissection in yeast showed it is required for actin-independent polarized localization of Exo70 and for recruiting subunits Sec5/Sec6 into the exocyst, genetically paralleling Sec3's Cdc42/Rho1-binding function and revealing redundancy in exocyst targeting.

    Evidence Domain deletion, synthetic lethality, subunit assembly, and localization assays in yeast

    PMID:18946089

    Open questions at the time
    • Binding partner of Domain C that mediates actin-independent localization not identified at this stage
  9. 2009 High

    TC10-dependent translocation of Exo70 to the distal axon was shown to be required for IGF-1-stimulated membrane expansion and axonal specification, establishing that GTPase-regulated exocyst targeting is a core mechanism of neuronal polarity.

    Evidence Dominant-negative and siRNA approaches, membrane fractionation, and live imaging in hippocampal neurons

    PMID:19846717

    Open questions at the time
    • Whether Exo70 tethers specific cargo vesicles during axon specification unknown
    • Relationship to other polarity determinants (Par complex) not addressed
  10. 2009 Medium

    Single-vesicle TIRF imaging demonstrated that Exo70 mediates insulin-stimulated tethering of GLUT4 vesicles at the plasma membrane as a step separable from fusion, mechanistically positioning exocyst function upstream of SNARE-dependent membrane merger.

    Evidence TIRF microscopy with Exo70 overexpression and dominant-negative mutant in primary adipocytes

    PMID:19155211

    Open questions at the time
    • Identity of the fusion trigger acting after Exo70-mediated tethering not defined
    • Contribution of individual exocyst subunits to tethering vs. fusion not separated
  11. 2012 High

    Reconstitution of Exo70 as a kinetic activator of Arp2/3-mediated actin branching—operating by facilitating WAVE2–Arp2/3 interaction—provided the biochemical mechanism for Exo70's role in lamellipodia formation and directional cell migration.

    Evidence In vitro actin polymerization, TIRF, Co-IP, and live cell migration assays

    PMID:22748316

    Open questions at the time
    • Binding interface between Exo70 and Arp2/3 not structurally resolved
    • How Exo70 coordinates simultaneous vesicle tethering and Arp2/3 activation unclear
  12. 2012 High

    ERK1/2 was identified as a direct kinase for Exo70 that enhances exocyst complex assembly upon growth factor signaling, linking MAP kinase signaling to regulated exocytosis, MMP secretion, and invadopodia formation in tumor cells.

    Evidence In vitro kinase assay, phosphorylation-deficient mutants, chemical inhibitors, exocytosis assay, and Co-IP

    PMID:22595671

    Open questions at the time
    • Specific ERK1/2 phosphorylation sites on Exo70 and structural consequences not fully mapped
    • Whether ERK phosphorylation also regulates Exo70-Arp2/3 interaction not tested
  13. 2013 High

    Demonstration that Exo70 oligomerizes to generate negative membrane curvature—producing tubular protrusions both in vitro and in cells—revealed a direct membrane-deforming activity, adding a biophysical mechanism beyond vesicle tethering and Arp2/3 activation.

    Evidence In vitro membrane tubulation with synthetic vesicles, mutagenesis, molecular dynamics, and live cell imaging

    PMID:23948253

    Open questions at the time
    • Physiological regulation of Exo70 oligomerization not defined
    • Whether membrane deformation cooperates with or is independent of Arp2/3 activation unclear
  14. 2013 High

    ESRP1-regulated alternative splicing of Exo70 was shown to produce distinct epithelial and mesenchymal isoforms; only the mesenchymal isoform engages Arp2/3 and stimulates actin polymerization, establishing isoform switching as a determinant of invasive behavior during EMT.

    Evidence Isoform-specific constructs, Co-IP with Arp2/3, in vitro actin polymerization, invasion assays, and mouse tumor metastasis model

    PMID:24331928

    Open questions at the time
    • Structural difference between isoforms that alters Arp2/3 binding not resolved
    • Whether isoform switching affects vesicle tethering function untested
  15. 2014 High

    Identifying the polarity scaffold Bem1p as a direct Exo70 binding partner required for actin-independent polarized localization—synergizing with PI(4,5)P2—resolved how Exo70 achieves robust targeting to exocytic sites independent of the actin cytoskeleton.

    Evidence Purified protein pulldown, domain mutants, genetic epistasis, and localization microscopy in yeast

    PMID:25313406

    Open questions at the time
    • Mammalian Bem1 ortholog interaction not demonstrated
    • Whether Bem1 and Rho3 binding are cooperative or competitive unknown
  16. 2018 High

    Drosophila Exo70 mutant analysis showed genetic interaction with RalA and a requirement for activity-induced synaptic outgrowth and surface protein delivery at NMJ terminals, validating a conserved RalA–exocyst axis in synaptic plasticity.

    Evidence Drosophila exo70 mutant alleles, genetic epistasis with ralA, electrophysiology, and surface trafficking assays

    PMID:30209205

    Open questions at the time
    • Biochemical mechanism of RalA–Exo70 interaction in Drosophila not defined
    • Whether Exo70 deforms membrane at synaptic terminals untested
  17. 2019 High

    Inducible knockout of Exoc7 in adipocytes confirmed that Exo70 is specifically required for insulin-stimulated GLUT4 exocytosis without affecting upstream insulin signaling, definitively placing the exocyst downstream of insulin receptor activation.

    Evidence Inducible CRISPR/Cas9 knockout with GLUT4 translocation and insulin signaling assays in adipocytes

    PMID:31740584

    Open questions at the time
    • Which exocyst assembly step is rate-limiting for GLUT4 exocytosis not determined
    • Compensation by other exocyst subunits not assessed
  18. 2020 High

    ULK1 was shown to directly phosphorylate Exo70, inhibiting its homo-oligomerization and exocyst assembly, thereby suppressing protrusion formation and invasion; this opposes ERK1/2 phosphorylation, establishing a phosphorylation-based toggle controlling exocyst activity.

    Evidence In vitro kinase assay, phospho-mutants, Co-IP for complex assembly, and invasion assays

    PMID:31913283

    Open questions at the time
    • Phosphorylation site interplay (which sites are modified by each kinase) not fully mapped
    • Whether ULK1 regulation connects to autophagy-dependent Exo70 degradation unclear
  19. 2020 Medium

    Human genetic studies and zebrafish knockout linked partial EXOC7 loss-of-function to a recessive neurodevelopmental disorder with microcephaly and seizures, demonstrating that Exo70 is essential for neural progenitor proliferation and cortical development.

    Evidence Zebrafish exoc7 knockout, exome sequencing in human families, and splice variant modeling

    PMID:32103185

    Open questions at the time
    • Cellular mechanism underlying neural progenitor defects not defined
    • Whether disease alleles affect exocyst assembly, Arp2/3 activation, or both unknown
  20. 2024 High

    Identification of TGM1/3-mediated transamidation of Exo70 at Gln5 as a modification that enhances exocyst assembly and is antagonized by LKB1 phosphorylation of TGM1/3 revealed a novel post-translational regulatory layer governing invasive secretion.

    Evidence Mass spectrometry PTM identification, in vitro transglutaminase assay, mutagenesis, Co-IP, invasion assays, and mouse model

    PMID:39146185

    Open questions at the time
    • Structural consequence of transamidation on Exo70 conformation unknown
    • Whether transamidation intersects with ERK/ULK1 phosphorylation not tested

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis of Exo70 within the assembled mammalian exocyst holocomplex, how Exo70 simultaneously coordinates vesicle tethering, Arp2/3 activation, and membrane deformation at the same site, and the pathomechanism linking specific EXOC7 variants to neurodevelopmental disease.
  • No high-resolution structure of mammalian Exo70 in the exocyst holocomplex
  • Mechanism coordinating tethering, Arp2/3 activation, and membrane deformation at single sites unknown
  • Cellular pathology of EXOC7 disease variants in neural progenitors not characterized

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0008092 cytoskeletal protein binding 3 GO:0060090 molecular adaptor activity 2 GO:0008289 lipid binding 1
Localization
GO:0005886 plasma membrane 5 GO:0031410 cytoplasmic vesicle 3 GO:0005829 cytosol 2
Pathway
R-HSA-5653656 Vesicle-mediated transport 5 R-HSA-162582 Signal Transduction 4 R-HSA-1266738 Developmental Biology 3 R-HSA-112316 Neuronal System 2 R-HSA-1500931 Cell-Cell communication 1
Partners
ARPC2BEM1CDC42PRPF19RHO3SEC5TC10WASF2
Complex memberships
Arp2/3 complex (functional activator, not stable subunit)exocyst complex

Evidence

Reading pass · 34 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2007 Exo70 C-terminal Domain D directly interacts with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) at the plasma membrane, and this interaction (but not its interaction with Rho3) is essential for membrane association of the exocyst complex and tethering of post-Golgi secretory vesicles. Direct lipid-binding assays, key residue mutagenesis, genetic and cell biological analyses in yeast The EMBO journal High 17717527
2004 Exo70p (and Sec3p) are stable plasma membrane components of the exocyst, whereas the other six subunits arrive on secretory vesicles; exocyst assembly occurs when vesicle-borne subunits join Exo70p and Sec3p at the plasma membrane to tether vesicles to exocytic sites. FRAP (photobleaching recovery), actin disruption, immunogold EM, epifluorescence video microscopy in yeast The Journal of cell biology High 15583031
1999 Exo70 interacts with the yeast GTPase Rho3 in a GTP-dependent manner; this interaction requires the Rho3 effector domain and was demonstrated with purified proteins in vitro. Overlapping subcellular localization of Rho3 and Exo70 was confirmed by immunofluorescence. Yeast two-hybrid screen, purified protein binding assay, indirect immunofluorescence Molecular and cellular biology High 10207081
2006 Human Exo70 directly interacts with the Arp2/3 complex, and this interaction is regulated by EGF signaling. Inhibition of Exo70 by RNAi or antibody microinjection blocks actin-based membrane protrusion formation and impairs cell motility. Co-immunoprecipitation, RNAi knockdown, antibody microinjection, live cell imaging Nature cell biology High 17086175
2005 Crystal structure of yeast Exo70p determined at 2.0 Å resolution reveals an ~160 Å rod composed of contiguous α-helical bundles (novel fold). Structural analysis combined with binding experiments shows Exo70p binds Rho3p in a GTP-dependent manner (Kd ~70 µM) and interacts with other exocyst subunits. X-ray crystallography (2.0 Å), equilibrium binding assay Nature structural & molecular biology High 16249794
2005 Crystal structure of S. cerevisiae Exo70p determined at 3.5 Å resolution shows an extended rod (~155 Å) composed principally of α-helices with a novel fold; Exo70p binds Rho3p GTPase in a GTP-dependent manner. X-ray crystallography (3.5 Å), GTPase binding assay Journal of molecular biology High 16359701
2012 ERK1/2 directly phosphorylate Exo70 in response to EGF signaling, enhancing Exo70 binding to other exocyst subunits and promoting exocyst complex assembly. Blocking ERK1/2 signaling or expressing an ERK1/2-phosphorylation-deficient Exo70 mutant inhibits exocytosis, matrix metalloproteinase secretion, and invadopodia formation in tumor cells. In vitro kinase assay, phosphorylation-deficient mutant expression, chemical inhibitor treatment, exocytosis assay, Co-IP Developmental cell High 22595671
2013 Exo70 generates negative membrane curvature through an oligomerization-based mechanism, inducing tubular membrane invaginations in synthetic vesicles in vitro and protrusions on cell surfaces. This membrane-deformation function requires specific residues identified by mutagenesis and is necessary for protrusion formation and directional cell migration. In vitro membrane tubulation assay with synthetic vesicles, Exo70 mutant analysis, molecular dynamics simulation, live cell imaging Developmental cell High 23948253
2013 Exo70 undergoes isoform switching regulated by the splicing factor ESRP1 during EMT. The mesenchymal isoform (but not the epithelial isoform) interacts with the Arp2/3 complex and stimulates actin polymerization to drive tumor invasion. Isoform-specific expression constructs, Co-IP with Arp2/3, RNAi, in vitro actin polymerization assay, invasion assays, mouse tumor metastasis model Developmental cell High 24331928
2012 Exo70 functions as a kinetic activator of the Arp2/3 complex, promoting actin filament nucleation and branching by facilitating interaction of Arp2/3 with WAVE2. This stimulatory activity is required for lamellipodia formation and maintaining directional persistence during cell migration. In vitro actin polymerization assay, TIRF microscopy, Co-IP, RNAi knockdown, live cell migration assay Current biology : CB High 22748316
2009 The TC10-Exo70 complex is required for IGF-1-stimulated membrane expansion and axonal specification in hippocampal neurons. IGF-1 activates TC10, which triggers translocation of Exo70 to the plasma membrane in the distal axon and growth cone; TC10 and Exo70 function are necessary for new membrane addition and polarized insertion of IGF-1 receptor. Dominant-negative and siRNA knockdown of TC10 and Exo70, plasma membrane fractionation, immunofluorescence, live imaging in cultured neurons and isolated growth cones The Journal of neuroscience High 19846717
2020 ULK1 directly phosphorylates Exo70, inhibiting Exo70 homo-oligomerization and its assembly into the exocyst complex, thereby suppressing cell protrusion formation, MMP secretion, and cancer cell invasion. ERK1/2 phosphorylation of Exo70 counteracts ULK1 phosphorylation upon growth factor stimulation. In vitro kinase assay, phosphorylation-deficient and phospho-mimetic mutants, Co-IP for complex assembly, invasion and migration assays Nature communications High 31913283
2007 Exo70p in yeast selectively mediates secretion of Bgl2p vesicles (one class of post-Golgi vesicles) primarily during early stages of the cell cycle; this selective block does not affect vesicle formation or sorting but occurs at the tethering/fusion step. Genetic analysis of exo70 mutants, vesicle accumulation assays, cargo-specific secretion assays in yeast The Journal of cell biology High 17339375
2005 Exo70 interacts with BIG2 (a brefeldin A-inhibited GEF for ARFs); the interaction involves the N-terminal segment of BIG2 (aa 1–643). Both BIG2 and Exo70 colocalize at Golgi membranes and the MTOC/centrosome in HepG2 cells. Yeast two-hybrid screen, co-immunoprecipitation of in vitro-translated proteins, immunofluorescence microscopy, centrosome purification PNAS Medium 15705715
2007 Exo70 interacts with Snapin via Exo70's N-terminal coiled-coil domain and Snapin's C-terminal helical region; Exo70 competes with SNAP23 for Snapin binding. Depletion of Snapin by RNAi inhibits insulin-stimulated glucose uptake in adipocytes, implicating this interaction in GLUT4 vesicle trafficking. Co-IP, domain mapping, competition assay, RNAi knockdown, glucose uptake assay in adipocytes The Journal of biological chemistry Medium 17947242
2011 Exo70 directly interacts with type Iγ PIP5-kinase (PIPKIγ), which mediates association of E-cadherin with Exo70 and targets Exo70 to adherens junctions. Exo70 is necessary for E-cadherin clustering and extension of nascent E-cadherin adhesions; PIPKIγ-generated PI(4,5)P2 recruits Exo70 to nascent E-cadherin junctions. Co-IP, pulldown, RNAi knockdown, confocal microscopy, PI(4,5)P2 localization assay Molecular biology of the cell Medium 22049025
2009 Exo70 mediates insulin-stimulated tethering of GLUT4 vesicles to the plasma membrane in primary rat adipocytes; an Exo70-N mutant induces insulin-independent tethering but not fusion, indicating that Exo70-mediated tethering is upstream of and separable from the insulin-regulated fusion step. TIRF microscopy for single vesicle tracking, Exo70 overexpression and dominant-negative mutant in primary adipocytes The Journal of biological chemistry Medium 19155211
2019 Inducible knockout of Exoc7 in adipocytes markedly inhibits insulin-stimulated GLUT4 exocytosis without affecting insulin signaling, demonstrating a critical and specific role for the exocyst (via Exo70) in GLUT4 vesicle exocytosis downstream of insulin receptor signaling. Inducible CRISPR/Cas9 knockout in adipocytes, GLUT4 translocation assay, insulin signaling assessment The Journal of biological chemistry High 31740584
2008 Domain C of yeast Exo70p (while not essential for growth) is required for actin-independent localization of Exo70p to exocytic sites and for assembly of Sec5p and Sec6p into the exocyst. Loss of domain C causes synthetic lethality with secretory mutations analogous to loss of Sec3p's Cdc42/Rho1-binding domain. Domain deletion analysis, genetic epistasis (synthetic lethality), exocyst subunit assembly assay, localization microscopy in yeast Molecular biology of the cell High 18946089
2014 Exo70p directly and specifically binds the polarity scaffold Bem1p via multiple domains of both proteins. Mutations disrupting Exo70p-Bem1p interaction abolish actin-independent localization of Exo70p. Actin-independent localization requires a synergistic interaction with PI(4,5)P2. Yeast two-hybrid, purified protein pulldown, domain deletion mutants, genetic epistasis, localization microscopy The Journal of cell biology High 25313406
2011 Exo70 interacts directly with SNEV (hPrp19/hPso4) via its N-terminal 100 amino acids and shuttles to the nucleus where it associates with the spliceosome. Exo70 influences pre-mRNA splicing in vitro and modulates splicing of its own pre-mRNA in vivo. Co-IP, nuclear fractionation, in vitro splicing assay, domain mapping, in vivo splicing reporter The Biochemical journal Medium 21639856
2007 NGF induces interaction of activated TC10 with Exo70 in PC12 cells (shown by FRET-FLIM). The Exo70-TC10 complex locally antagonizes Cdc42-mediated activation of N-WASP at membrane protrusions to regulate the form of membrane outgrowth during neurite formation. FRET-FLIM imaging, dominant-negative mutants, siRNA knockdown, N-WASP activation biosensor Journal of cell science Medium 17635999
2009 Exo70 recruits nucleoporin Nup62 to the leading edge of migrating cells through direct binding involving the N-terminal domain of Exo70 and the coiled-coil domain of Nup62. RNAi silencing of Nup62 significantly reduces cell migration; removal of the Exo70-binding domain of Nup62 prevents its leading edge localization. Co-IP, domain mapping pulldown, RNAi knockdown, confocal microscopy Traffic (Copenhagen, Denmark) Medium 19552648
2013 GTP hydrolysis of vesicular TC10 near the plasma membrane releases Exo70, accelerating vesicle fusion and promoting neurite outgrowth. Constitutively active TC10 cannot rescue neurite outgrowth defects caused by TC10 depletion, indicating that the GTPase cycle is required. FRET biosensors for TC10 activity, live cell imaging of vesicle fusion, TC10 knockdown and rescue experiments in neuronal cells PloS one Medium 24223996
2020 Loss of exoc7 in zebrafish causes microcephaly, and EXOC7 partial loss-of-function variants in humans cause a recessive disorder of cerebral cortex development with brain atrophy, seizures, and in severe cases microcephaly; EXOC7 is required for neural progenitor cell proliferation and survival. Zebrafish exoc7 knockout, exome sequencing in human families, EXOC7 splice variant modeling in vitro Genetics in medicine Medium 32103185
2021 Exoc7 (Exo70) interacts with the E3 ubiquitin ligase Prpf19 and modulates levels of polyglutamine-expanded ataxin-3 (ATXN3-polyQ) in an opposite manner to Prpf19; Exoc7 exerts its effect by regulating the E3 ligase function of Prpf19 on ATXN3-polyQ ubiquitination and degradation. Co-IP, Exoc7 knockdown in mammalian and Drosophila models, polyQ protein level and toxicity assays, genetic epistasis Cell death & disease Medium 33542212
2021 Exo70 contributes to cisplatin resistance in ovarian cancer cells by promoting exocytosis-mediated efflux of cisplatin. Cisplatin induces autophagy-lysosomal degradation of Exo70 via AMPK/mTOR phosphorylation modulation; stabilization of Exo70 under prolonged cisplatin treatment drives acquired resistance. Exo70 knockdown/overexpression, cisplatin efflux assay, autophagy-lysosome pathway analysis, in vitro and in vivo tumor models Cancers Medium 34298686
2024 Exo70 is transamidated on Gln5 (with Lys56 of cystatin A) by transglutaminases TGM1 and TGM3; this modification enhances Exo70's assembly with other exocyst subunits, MMP secretion, invadopodia formation, and integrin delivery. Tumor suppressor LKB1 phosphorylates TGM1/TGM3 to inhibit their interaction with Exo70 and block this transamidation. Mass spectrometry for PTM identification, mutagenesis, Co-IP for complex assembly, in vitro transglutaminase assay, invasion assays, in vivo mouse model Cell reports High 39146185
2022 The brain-specific Cdc42b isoform (but not ubiquitous Cdc42u) interacts with Exo70 in mammalian neurons. Inactivation of Arhgef7 or Cdc42b interferes with exocytosis of post-Golgi vesicles in the growth cone and with axon formation, placing Cdc42b upstream of Exo70-dependent exocytosis in neuronal polarity. Isoform-specific interaction assays, Arhgef7/Cdc42b conditional inactivation, live imaging of post-Golgi vesicle exocytosis in growth cones Life science alliance Medium 36543541
2015 GIV/Girdin directly and constitutively binds the exocyst subunit Exo70 and associates with GLUT4-storage vesicles upon insulin stimulation. Without GIV or its GEF function, membrane association of Exo70 and insulin-stimulated exocytosis of GLUT4 vesicles are impaired. Pulldown, Co-IP, subcellular fractionation, GLUT4 exocytosis assay in adipocytes Biochemical and biophysical research communications Medium 26514725
2021 Exo70 undergoes redistribution from the plasma membrane microsomal fraction to the synaptic compartment after traumatic brain injury; in the synaptic compartment, exocyst complex assembly and its interaction with GluN2B are increased, suggesting Exo70 modulates GluN2B synaptic availability after brain trauma. Subcellular fractionation, Co-IP of Exo70 with GluN2B, immunoblotting after repeated mild TBI model Biological research Medium 33593425
2024 Exo70 promotes pancreatic cancer cell invasion and metastasis by regulating secretion of tumor exosomes; Exo70 knockdown or ES2 inhibitor treatment inhibits exosome secretion and increases accumulation of cellular vesicles. Exo70 also increases exosomal PD-L1 expression, contributing to immune escape. Exo70 knockdown, ES2 inhibitor treatment, nanoparticle tracking for exosome quantification, in vivo mouse metastasis model Cancers Medium 38254825
2025 Cytoplasmic METTL3 interacts with EXOC7 and stabilizes it; METTL3 also regulates m6A-dependent alternative splicing of EXOC7 promoting expression of a more aggressive isoform. Silencing METTL3 (but not inhibiting its catalytic activity) impairs vesicle trafficking, the breast cancer secretome, and invadopodia formation. Co-IP, RIP, METTL3 knockdown vs. catalytic inhibition, vesicle trafficking assay, invasion assay bioRxivpreprint Medium bio_10.1101_2025.05.26.656168
2018 In Drosophila, Exo70 genetically interacts with ralA to regulate synaptic growth at the NMJ. Loss of Exo70 blocks JNK signaling-, activity-, and temperature-induced synaptic outgrowths and impairs integral membrane protein transport to the cell surface at synaptic terminals. Drosophila exo70 mutant alleles, genetic epistasis with ralA, electrophysiology, live imaging, surface protein trafficking assay The Journal of neuroscience High 30209205

Source papers

Stage 0 corpus · 72 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2007 Exo70 interacts with phospholipids and mediates the targeting of the exocyst to the plasma membrane. The EMBO journal 258 17717527
2004 Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p. The Journal of cell biology 241 15583031
1999 Rho3 of Saccharomyces cerevisiae, which regulates the actin cytoskeleton and exocytosis, is a GTPase which interacts with Myo2 and Exo70. Molecular and cellular biology 163 10207081
2006 Exo70 interacts with the Arp2/3 complex and regulates cell migration. Nature cell biology 147 17086175
2015 Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis. Proceedings of the National Academy of Sciences of the United States of America 123 26607451
2005 The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif. Nature structural & molecular biology 119 16249794
2015 Rice Exo70 interacts with a fungal effector, AVR-Pii, and is required for AVR-Pii-triggered immunity. The Plant journal : for cell and molecular biology 100 26186703
2009 The TC10-Exo70 complex is essential for membrane expansion and axonal specification in developing neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience 93 19846717
2013 Exo70 generates membrane curvature for morphogenesis and cell migration. Developmental cell 85 23948253
2010 Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis. Plant physiology 81 20943851
2005 Crystal structure of the S.cerevisiae exocyst component Exo70p. Journal of molecular biology 68 16359701
2012 ERK1/2 regulate exocytosis through direct phosphorylation of the exocyst component Exo70. Developmental cell 66 22595671
2013 Exo70 isoform switching upon epithelial-mesenchymal transition mediates cancer cell invasion. Developmental cell 62 24331928
2007 Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth. The Journal of cell biology 57 17339375
2012 Fission yeast Sec3 and Exo70 are transported on actin cables and localize the exocyst complex to cell poles. PloS one 55 22768263
2017 Analysis of Exocyst Subunit EXO70 Family Reveals Distinct Membrane Polar Domains in Tobacco Pollen Tubes. Plant physiology 50 28082718
2011 An association between type Iγ PI4P 5-kinase and Exo70 directs E-cadherin clustering and epithelial polarization. Molecular biology of the cell 49 22049025
2020 ULK1 phosphorylates Exo70 to suppress breast cancer metastasis. Nature communications 47 31913283
2012 Exo70 stimulates the Arp2/3 complex for lamellipodia formation and directional cell migration. Current biology : CB 46 22748316
2013 GTP hydrolysis of TC10 promotes neurite outgrowth through exocytic fusion of Rab11- and L1-containing vesicles by releasing exocyst component Exo70. PloS one 45 24223996
2005 Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70. Proceedings of the National Academy of Sciences of the United States of America 45 15705715
2007 Snapin interacts with the Exo70 subunit of the exocyst and modulates GLUT4 trafficking. The Journal of biological chemistry 40 17947242
2020 Three subfamilies of exocyst EXO70 family subunits in land plants: early divergence and ongoing functional specialization. Journal of experimental botany 36 31647563
2007 An NGF-induced Exo70-TC10 complex locally antagonises Cdc42-mediated activation of N-WASP to modulate neurite outgrowth. Journal of cell science 34 17635999
2009 Insulin regulates fusion of GLUT4 vesicles independent of Exo70-mediated tethering. The Journal of biological chemistry 29 19155211
2009 Exo70-mediated recruitment of nucleoporin Nup62 at the leading edge of migrating cells is required for cell migration. Traffic (Copenhagen, Denmark) 29 19552648
2022 A blast fungus zinc-finger fold effector binds to a hydrophobic pocket in host Exo70 proteins to modulate immune recognition in rice. Proceedings of the National Academy of Sciences of the United States of America 28 36252011
2019 Inducible Exoc7/Exo70 knockout reveals a critical role of the exocyst in insulin-regulated GLUT4 exocytosis. The Journal of biological chemistry 27 31740584
2008 An internal domain of Exo70p is required for actin-independent localization and mediates assembly of specific exocyst components. Molecular biology of the cell 27 18946089
2019 Arabidopsis Trichome Contains Two Plasma Membrane Domains with Different Lipid Compositions Which Attract Distinct EXO70 Subunits. International journal of molecular sciences 26 31382643
2017 The Physcomitrella patens exocyst subunit EXO70.3d has distinct roles in growth and development, and is essential for completion of the moss life cycle. The New phytologist 26 28397275
2018 Identification and Characterization of the EXO70 Gene Family in Polyploid Wheat and Related Species. International journal of molecular sciences 24 30586859
2016 Expression and Functional Analysis of a Novel Group of Legume-specific WRKY and Exo70 Protein Variants from Soybean. Scientific reports 24 27572297
2014 Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p. The Journal of cell biology 24 25313406
2020 Regulation of human cerebral cortical development by EXOC7 and EXOC8, components of the exocyst complex, and roles in neural progenitor cell proliferation and survival. Genetics in medicine : official journal of the American College of Medical Genetics 23 32103185
2017 The role of Exo70 in exocytosis and beyond. Small GTPases 20 28489961
2022 A lineage-specific Exo70 is required for receptor kinase-mediated immunity in barley. Science advances 19 35857460
2011 Exo70, a subunit of the exocyst complex, interacts with SNEV(hPrp19/hPso4) and is involved in pre-mRNA splicing. The Biochemical journal 19 21639856
2018 The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons. Molecular neurobiology 18 30374940
2010 EXO70 protein influences dengue virus secretion. Microbes and infection 17 21034848
2016 The role of Exo70 in vascular smooth muscle cell migration. Cellular & molecular biology letters 16 28536622
2012 Exo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1. PloS one 16 23300727
2024 Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. The Plant cell 15 38124479
2016 Cortactin and Exo70 mediated invasion of hepatoma carcinoma cells by MMP-9 secretion. Molecular biology reports 15 27025610
2021 Functional Specialization within the EXO70 Gene Family in Arabidopsis. International journal of molecular sciences 14 34299214
2020 Appraising the Value of Serum and Serum-Derived Exosomal LncRNA-EXOC7 as a Promising Biomarker in Cervical Cancer. Clinical laboratory 14 32658426
2020 Redundant and Diversified Roles Among Selected Arabidopsis thaliana EXO70 Paralogs During Biotic Stress Responses. Frontiers in plant science 14 32676093
2012 Exocyst subunits Exo70 and Exo84 cooperate with small GTPases to regulate behavior and endocytic trafficking in C. elegans. PloS one 14 22389680
2015 GIV/girdin binds exocyst subunit-Exo70 and regulates exocytosis of GLUT4 storage vesicles. Biochemical and biophysical research communications 13 26514725
2018 Drosophila Exo70 Is Essential for Neurite Extension and Survival under Thermal Stress. The Journal of neuroscience : the official journal of the Society for Neuroscience 12 30209205
2015 Identification and characterization of a novel group of legume-specific, Golgi apparatus-localized WRKY and Exo70 proteins from soybean. Journal of experimental botany 12 25805717
2021 Deregulation of Exo70 Facilitates Innate and Acquired Cisplatin Resistance in Epithelial Ovarian Cancer by Promoting Cisplatin Efflux. Cancers 11 34298686
2021 A fine balance between Prpf19 and Exoc7 in achieving degradation of aggregated protein and suppression of cell death in spinocerebellar ataxia type 3. Cell death & disease 10 33542212
2016 Exo70 is transcriptionally up-regulated by hepatic nuclear factor 4α and contributes to cell cycle control in hepatoma cells. Oncotarget 8 26848864
2021 Exo70 intracellular redistribution after repeated mild traumatic brain injury. Biological research 7 33593425
2019 Kinase suppressor of Ras 1 and Exo70 promote fatty acid-stimulated neurotensin secretion through ERK1/2 signaling. PloS one 7 30917119
2010 Different steps of sexual development are differentially regulated by the Sec8p and Exo70p exocyst subunits. FEMS microbiology letters 7 20180855
2024 Exo70 Promotes the Invasion of Pancreatic Cancer Cells via the Regulation of Exosomes. Cancers 6 38254825
2021 Identification and Comprehensive Structural and Functional Analyses of the EXO70 Gene Family in Cotton. Genes 6 34680988
2024 TGM1/3-mediated transamidation of Exo70 promotes tumor metastasis upon LKB1 inactivation. Cell reports 5 39146185
2023 The EXO70 inhibitor Endosidin2 alters plasma membrane protein composition in Arabidopsis roots. Frontiers in plant science 5 37324680
2021 Genome-wide identification and expression analysis of the EXO70 gene family in grape (Vitis vinifera L). PeerJ 5 33976971
2022 An isoform-specific function of Cdc42 in regulating mammalian Exo70 during axon formation. Life science alliance 4 36543541
2024 LncRNA ZNF649-AS1 promotes trastuzumab resistance and TAM-dependent PD-L1 expression in breast cancer by regulating EXOC7 alternative splicing. Archives of biochemistry and biophysics 3 39159899
2023 Genome-Wide Analysis of Exocyst Complex Subunit Exo70 Gene Family in Cucumber. International journal of molecular sciences 3 37446106
2025 ELABELA promotes the migration and homing of bone marrow mesenchymal stem cells to myocardial injury sites through the ERK1/2/miR-299a-5p/Exo70 pathway. Frontiers in pharmacology 2 39963243
2020 GIV/Girdin and Exo70 Collaboratively Regulate the Mammalian Polarized Exocytic Machinery. iScience 2 32590327
2024 Deletion of Exoc7, but not Exoc3, in male germ cells causes severe spermatogenesis failure with spermatocyte aggregation in mice. Experimental animals 1 38325858
2024 A chemical genetic screen with the EXO70 inhibitor Endosidin2 uncovers potential modulators of exocytosis in Arabidopsis. Plant direct 1 38881683
2026 Genome-wide identification of the EXO70 genes to elucidate their potential roles for intraspecific cross-incompatibility in sweet potato (Ipomoea batatas L.). Frontiers in plant science 0 41835271
2025 Exo70 Protects Against Memory and Synaptic Impairments Following Mild Traumatic Brain Injury. Antioxidants (Basel, Switzerland) 0 40563275
2024 TC10 differently controls the dynamics of Exo70 in growth cones of cortical and hippocampal neurons. Biophysical reports 0 39521348