Affinage

EXOC7

Exocyst complex component 7 · UniProt Q9UPT5

Length
735 aa
Mass
83.4 kDa
Annotated
2026-06-09
68 papers in source corpus 37 papers cited in narrative 38 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

EXOC7/Exo70 is a subunit of the octameric exocyst tethering complex that captures post-Golgi secretory vesicles at the plasma membrane for SNARE-mediated fusion, and which doubles as a direct effector of actin remodeling during cell migration (PMID:15583031, PMID:17086175, PMID:17339375). Exo70 anchors the exocyst to the plasma membrane through direct binding of its C-terminal Domain D to PI(4,5)P2, an interaction essential for exocyst membrane association and, together with Domain C, for actin-independent localization of Exo70 to exocytic sites (PMID:17717527, PMID:18946089). Crystal structures reveal an extended ~160 Å rod of contiguous alpha-helical bundles whose C-terminal modules mediate binding to other exocyst subunits and to the GTP-loaded Rho3 GTPase (PMID:16249794, PMID:16359701, PMID:10207081); in yeast this membrane targeting also requires the polarity scaffold Bem1p, a role fulfilled in mammalian cells by GIV/Girdin (PMID:25313406, PMID:32590327). Beyond tethering, Exo70 acts as a kinetic activator of the Arp2/3 complex by facilitating its engagement with WAVE2 to drive actin branching, and independently deforms membranes into negative curvature via oligomerization to build protrusions and direct cell migration (PMID:22748316, PMID:23948253, PMID:17086175). Exo70 function is gated by opposing post-translational modifications: ERK1/2 phosphorylation and TGM1/3 transamidation each promote exocyst assembly and MMP/invadopodia-driven invasion, whereas ULK1 phosphorylation inhibits Exo70 oligomerization and assembly (PMID:22595671, PMID:39146185, PMID:31913283). Exocyst-mediated trafficking through Exo70 is required for insulin-stimulated GLUT4 exocytosis in adipocytes, for axon and neurite outgrowth downstream of TC10 and the brain-specific Cdc42b GTPase, and for E-cadherin junction maturation (PMID:31740584, PMID:19846717, PMID:36543541, PMID:22049025). An EMT-associated isoform switch controlled by ESRP1 partitions these activities between epithelial and invasive mesenchymal programs (PMID:24331928). Loss-of-function variants in EXOC7 cause brain atrophy, seizures, and developmental delay, with zebrafish exoc7 loss producing microcephaly (PMID:32103185).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1999 High

    Establishing that a small GTPase directly engages Exo70 placed the protein within polarized GTPase signaling rather than being a passive structural subunit.

    Evidence Yeast two-hybrid and in vitro pulldown with purified Rho3, with GTP/GDP loading and localization in budding yeast

    PMID:10207081

    Open questions at the time
    • Did not define the membrane-targeting determinants of Exo70
    • Functional consequence of Rho3 binding for vesicle tethering not resolved
  2. 2004 High

    Live imaging answered how the exocyst assembles by showing Exo70 marks the plasma-membrane landmark to which vesicle-borne subunits dock, defining a spatial logic for tethering.

    Evidence FRAP, immunogold EM, and live video microscopy with actin disruption in budding yeast

    PMID:15583031

    Open questions at the time
    • Molecular basis of actin-independent membrane anchoring not yet identified
    • Mechanism of vesicle subunit recruitment unresolved
  3. 2005 High

    High-resolution structures defined the Exo70 fold as an extended alpha-helical rod, providing the architecture for its subunit and GTPase contacts.

    Evidence X-ray crystallography of yeast Exo70p at 2.0 Å and 3.5 Å with Rho3 binding (Kd ~70 µM) and interaction mapping

    PMID:16249794 PMID:16359701

    Open questions at the time
    • No structure of the assembled exocyst octamer
    • Lipid-binding surface not localized structurally at this stage
  4. 2006 High

    Discovery of a direct Exo70–Arp2/3 interaction revealed a tethering subunit doubling as an actin-cytoskeleton regulator, linking secretion machinery to motility.

    Evidence Co-IP, RNAi knockdown, antibody microinjection, and cell migration assays under EGF signaling

    PMID:17086175

    Open questions at the time
    • Did not establish whether Arp2/3 regulation is separable from exocyst tethering
    • Biochemical mechanism of Arp2/3 modulation not defined
  5. 2007 High

    Identifying Domain D–PI(4,5)P2 binding answered how Exo70 anchors the exocyst to the plasma membrane independently of GTPases.

    Evidence In vitro lipid-binding assay, site-directed mutagenesis, and genetic/cell biology in yeast; vesicle-class selectivity defined by exo70 mutants

    PMID:17339375 PMID:17717527

    Open questions at the time
    • How lipid binding cooperates with protein landmarks for site selection not fully resolved
    • Vesicle-class selectivity mechanism (Bgl2p vs invertase) single-lab
  6. 2009 High

    Linking TC10 GTPase to Exo70 translocation defined an upstream signaling input driving polarized membrane addition during axon formation.

    Evidence siRNA and dominant-negative TC10/Exo70, subcellular fractionation, and imaging in primary hippocampal neurons and growth cones (IGF-1 stimulation)

    PMID:19846717

    Open questions at the time
    • Direct binding interface between TC10 and Exo70 not mapped
    • Coupling to specific cargo at growth cone not yet defined
  7. 2012 High

    Two studies resolved opposing biochemical roles in actin and assembly: Exo70 kinetically activates Arp2/3 via WAVE2, and ERK1/2 phosphorylation promotes exocyst assembly for secretion and invasion.

    Evidence In vitro actin polymerization and TIRF single-filament imaging; in vitro kinase assay with phospho-defective mutant and invadopodia/exocytosis readouts

    PMID:22595671 PMID:22748316

    Open questions at the time
    • How actin-nucleation and tethering activities are temporally coordinated unclear
    • ERK1/2 phospho-site relationship to other PTMs not yet defined
  8. 2013 High

    Mechanistic and isoform studies established that Exo70 directly deforms membranes by oligomerization and that an ESRP1-controlled isoform switch routes its activities into invasion versus epithelial programs.

    Evidence Liposome tubulation, mutagenesis, MD simulation, migration assays; RNA isoform analysis, isoform-specific Co-IP with Arp2/3, and mouse metastasis model

    PMID:23948253 PMID:24331928

    Open questions at the time
    • Relationship between curvature generation and Arp2/3 activation not integrated
    • Epithelial isoform's effect on Snail/ZEB2 mechanism not fully defined
  9. 2020 High

    Identifying ULK1 as an inhibitory kinase counteracting ERK1/2 defined a bidirectional phospho-switch governing Exo70 oligomerization and exocyst assembly.

    Evidence In vitro kinase assay, phospho-site mutagenesis, oligomerization and Co-IP assays, invasion assays

    PMID:31913283

    Open questions at the time
    • Upstream signals selecting ULK1 versus ERK1/2 dominance not defined
    • Quantitative stoichiometry of dual phosphorylation unresolved
  10. 2020 Medium

    Establishing GIV/Girdin as the mammalian Bem1p counterpart resolved how Exo70 is targeted by a polarity scaffold in metazoan cells, with direct relevance to MT1-MMP delivery.

    Evidence Motif-based interaction assay, Co-IP, and MT1-MMP trafficking/collagen degradation assays; complemented by yeast Bem1p direct-binding and separation-of-function mutants

    PMID:25313406 PMID:32590327

    Open questions at the time
    • Structural detail of the GIV/Bem1p short-linear-motif interface limited
    • In vivo requirement of GIV-Exo70 axis not tested
  11. 2020 Medium

    Genetic evidence linked EXOC7 to human neurodevelopment, defining a Mendelian disease association.

    Evidence Zebrafish exoc7 loss-of-function (microcephaly), in vitro splice-variant modeling, and human homozygosity mapping with exome sequencing

    PMID:32103185

    Open questions at the time
    • Cellular mechanism connecting exocyst defect to cortical phenotype not defined
    • Single study; allelic series limited
  12. 2024 High

    Discovery of TGM1/3 transamidation as an assembly-promoting PTM, antagonized by LKB1 phosphorylation, added a covalent regulatory layer and a druggable node for invasion.

    Evidence MS site identification, mutagenesis, in vitro transamidation and kinase assays, invadopodia/invasion assays, in vivo tumor model, cantharidin inhibition

    PMID:39146185

    Open questions at the time
    • Interplay of transamidation with phospho-switch not integrated
    • Generality across non-tumor contexts untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the distinct Exo70 activities — PI(4,5)P2 anchoring, vesicle tethering, Arp2/3 activation, and membrane curvature generation — are integrated and switched in space and time by its overlapping PTM and GTPase inputs remains unresolved.
  • No unified structural model coupling tethering and actin functions
  • Crosstalk among ERK1/2, ULK1, and TGM1/3 modifications in vivo undefined
  • Cargo-selection logic across tissues incompletely mapped

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 3 GO:0008092 cytoskeletal protein binding 2 GO:0008289 lipid binding 2 GO:0098772 molecular function regulator activity 1
Localization
GO:0005886 plasma membrane 3 GO:0005794 Golgi apparatus 2 GO:0005815 microtubule organizing center 1 GO:0005829 cytosol 1
Pathway
R-HSA-1266738 Developmental Biology 3 R-HSA-162582 Signal Transduction 3 R-HSA-5653656 Vesicle-mediated transport 3 R-HSA-9609507 Protein localization 3
Partners
ARPC (ARP2/3 COMPLEX)BEM1CDC42ESRP1GIV/CCDC88ANUP62RHO3TC10/RHOQ
Complex memberships
exocyst

Evidence

Reading pass · 38 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2007 The C-terminal Domain D of Exo70 directly interacts with phosphatidylinositol 4,5-bisphosphate (PI4,5P2), and key residues critical for this interaction were identified by mutagenesis. The interaction of Exo70 with phospholipids (but not Rho3) is essential for membrane association of the exocyst complex, anchoring it to the plasma membrane in concert with Sec3. In vitro lipid-binding assay, site-directed mutagenesis, genetic and cell biological analyses in yeast The EMBO journal High 17717527
2004 In budding yeast, Exo70p (together with Sec3p) is stably associated with exocytic sites at the plasma membrane independently of actin cables, while other exocyst subunits arrive on secretory vesicles. Exocyst assembly occurs when vesicle-borne subunits join Exo70p and Sec3p at the plasma membrane to tether vesicles. FRAP (fluorescence recovery after photobleaching), immunogold electron microscopy, epifluorescence video microscopy, actin disruption experiments The Journal of cell biology High 15583031
1999 Yeast Rho3 GTPase directly interacts with Exo70 in a GTP-dependent manner (GTPγS-bound Rho3 binds more efficiently than GDP-bound), as shown by yeast two-hybrid and in vitro pulldown with purified proteins. Rho3 and Exo70 co-localize at the bud tip, and dominant-active Rho3 alters Exo70 localization. Yeast two-hybrid screen, in vitro binding assay with purified proteins, GTP/GDP loading, indirect immunofluorescence Molecular and cellular biology High 10207081
2006 The exocyst component Exo70 directly interacts with the Arp2/3 complex. This interaction is regulated by EGF signalling. Inhibition of Exo70 by RNAi or antibody microinjection blocks formation of actin-based membrane protrusions and impairs cell motility. Co-immunoprecipitation, RNAi knockdown, antibody microinjection, cell migration assays Nature cell biology High 17086175
2005 Crystal structure of yeast Exo70p at 2.0 Å resolution reveals a ~160 Å-long rod composed of contiguous alpha-helical bundles (novel fold). The C-terminal domains interact with other exocyst subunits and Rho3p GTPase. Exo84p C-terminal domains share the same fold as the Exo70p N-terminus, suggesting a common helical module architecture for exocyst subunits. X-ray crystallography (2.0 Å resolution), structural interaction analysis Nature structural & molecular biology High 16249794
2005 Crystal structure of S. cerevisiae Exo70p at 3.5 Å resolution reveals an extended rod (~155 Å) composed principally of alpha helices. Exo70p binds Rho3p in a GTP-dependent manner with a Kd of ~70 µM. X-ray crystallography (3.5 Å), equilibrium binding assay (Kd determination) Journal of molecular biology High 16359701
2012 ERK1/2 directly phosphorylate the exocyst component Exo70. This phosphorylation enhances binding of Exo70 to other exocyst components and promotes exocyst complex assembly in response to EGF signalling. An Exo70 phosphorylation-defective mutant inhibits exocytosis, and in tumor cells blocks matrix metalloproteinase secretion and invadopodia formation. In vitro kinase assay, phosphorylation-defective mutant expression, Co-immunoprecipitation, exocytosis assay, invadopodia assay Developmental cell High 22595671
2013 Exo70 induces negative membrane curvature through an oligomerization-based mechanism. Exo70 generates tubular invaginations in synthetic vesicles in vitro and produces membrane protrusions on cell surfaces. The membrane-deformation function, validated by Exo70 mutants and molecular dynamics simulations, is required for protrusion formation and directional cell migration. In vitro liposome tubulation assay, Exo70 mutagenesis, molecular dynamics simulation, cell protrusion and migration assays Developmental cell High 23948253
2012 Exo70 functions as a kinetic activator of the Arp2/3 complex, promoting actin filament nucleation and branching by facilitating the interaction of Arp2/3 with the nucleation-promoting factor WAVE2. This activity is required for lamellipodia formation and directional persistence of cell migration. In vitro actin polymerization assay, TIRF microscopy, Co-immunoprecipitation, cell migration assays with Exo70 knockdown Current biology : CB High 22748316
2013 During epithelial-mesenchymal transition, Exo70 undergoes isoform switching regulated by the splicing factor ESRP1. The mesenchymal (but not epithelial) isoform of Exo70 interacts with the Arp2/3 complex and stimulates actin polymerization for tumor invasion. The epithelial isoform affects levels of EMT transcription factors Snail and ZEB2 and drives epithelial phenotypes. RNA isoform analysis, Co-immunoprecipitation, actin polymerization assay, cell invasion assays, mouse tumor metastasis model Developmental cell High 24331928
2020 ULK1 directly phosphorylates Exo70, and this phosphorylation inhibits Exo70 homo-oligomerization and its assembly into the exocyst complex, suppressing cell protrusion formation and MMP secretion during invasion. EGF stimulation causes ERK1/2 to phosphorylate Exo70 at a different site, which counteracts ULK1 phosphorylation—defining two opposing regulatory modifications. In vitro kinase assay, phosphorylation-site mutagenesis, oligomerization assay, Co-immunoprecipitation, cell invasion assays Nature communications High 31913283
2009 TC10 GTPase activates and triggers translocation of Exo70 to the plasma membrane in the distal axon and growth cone in response to IGF-1. TC10 and Exo70 function are both necessary for membrane addition and axon elongation stimulated by IGF-1, and for polarized insertion of the IGF-1 receptor to establish neuronal polarity. siRNA knockdown of TC10 and Exo70, dominant-negative expression, subcellular fractionation, immunofluorescence in cultured hippocampal neurons and isolated growth cones The Journal of neuroscience High 19846717
2007 Exo70p selectively mediates secretion of the Bgl2p class of post-Golgi vesicles in budding yeast, with secretion defect most pronounced at early stages of the cell cycle (early budding stage), affecting daughter cell growth. The block occurs at the tethering step, not vesicle formation or cargo sorting. Yeast genetics with exo70 mutants, secretion assays for Bgl2p vs. invertase vesicles, cell biological analysis The Journal of cell biology Medium 17339375
2011 Type Iγ PI4P 5-kinase (PIPKIγ) directly interacts with Exo70 and mediates association between E-cadherin and Exo70. PIPKIγ-generated PI4,5P2 recruits Exo70 to nascent E-cadherin junctions, and Exo70 is required for E-cadherin clustering and maturation of adherens junctions. Co-immunoprecipitation, direct binding assay, siRNA knockdown, fluorescence microscopy of E-cadherin junctions Molecular biology of the cell Medium 22049025
2005 BIG2 (a brefeldin A-inhibited ARF guanine nucleotide-exchange protein) interacts with the N-terminal portion (aa 1–643) of human Exo70. Endogenous BIG2 and Exo70 co-localize at trans-Golgi network membranes and at the MTOC/centrosomes in HepG2 cells. Yeast two-hybrid, co-immunoprecipitation of in vitro-translated proteins, immunofluorescence confocal microscopy, centrosome purification Proceedings of the National Academy of Sciences of the United States of America Medium 15705715
2007 Snapin interacts with the Exo70 subunit of the exocyst via an N-terminal coiled-coil domain of Exo70 and a C-terminal helical region of Snapin. Exo70 competes with SNAP23 for Snapin binding. Snapin depletion by RNAi inhibits insulin-stimulated glucose uptake in adipocytes, modulating GLUT4 vesicle trafficking. Co-immunoprecipitation, domain mapping, RNAi knockdown, glucose uptake assay The Journal of biological chemistry Medium 17947242
2009 Exo70 (Exo70-N mutant) induces insulin-independent tethering of GLUT4 vesicles to the plasma membrane in primary adipocytes, but this tethering does not lead to vesicle fusion without insulin. Insulin regulates the fusion step downstream of Exo70-mediated tethering. Total internal reflection fluorescence microscopy of GLUT4 vesicle dynamics, Exo70 overexpression and dominant mutant expression in primary adipocytes The Journal of biological chemistry Medium 19155211
2019 Inducible knockout of Exoc7/Exo70 in adipocytes markedly inhibits insulin-stimulated GLUT4 exocytosis without affecting insulin signaling, establishing that the exocyst (via Exo70) is required for the tethering/fusion step of GLUT4 vesicle exocytosis. CRISPR-based inducible adipocyte-specific Exoc7 knockout, GLUT4 translocation assay, insulin signaling assays The Journal of biological chemistry High 31740584
2009 Exo70 directly interacts with nucleoporin Nup62 via the N-terminal domain of Exo70. Exo70 recruits Nup62 to the leading edge of migrating cells and to filopodia. RNAi knockdown of Nup62 significantly reduces cell migration, and removal of the Exo70-binding domain from Nup62 prevents its leading-edge localization. Co-immunoprecipitation, domain mapping, RNAi knockdown, fluorescence microscopy, cell migration assay Traffic Medium 19552648
2008 Domain C of yeast Exo70p is required for actin-independent localization to exocytic sites and for assembly of exocyst components Sec5p and Sec6p. Deletion of domain C causes synthetic lethality with secretory mutations. The actin-independent localization requires a synergistic interaction with PI(4,5)P2 in addition to domain C. Yeast genetics, domain deletion analysis, synthetic lethality screening, exocyst assembly assay, fluorescence microscopy Molecular biology of the cell Medium 18946089
2014 Yeast Exo70p directly and specifically binds the polarity scaffold Bem1p through multiple domains of both proteins. Mutations in Exo70p that disrupt the Bem1p interaction without impairing other interactions abolish actin-independent localization of Exo70p to exocytic sites. Actin-independent localization requires both Bem1p interaction and PI(4,5)P2. In vitro binding assay (direct binding), Co-immunoprecipitation, mutagenesis, fluorescence microscopy, synthetic genetic analysis The Journal of cell biology High 25313406
2011 Exo70 directly interacts with the spliceosomal protein SNEV (hPrp19/hPso4), shuttles to the nucleus, and associates with the spliceosome. The N-terminal 100 amino acids of Exo70 mediate the interaction and interfere with pre-mRNA splicing in vitro. Exo70 influences splicing of a model substrate and of its own pre-mRNA in vivo. Co-immunoprecipitation, in vitro splicing assay, nuclear fractionation, domain mapping, in vivo splicing assay The Biochemical journal Medium 21639856
2013 TC10 GTP hydrolysis near the plasma membrane promotes neurite outgrowth by releasing Exo70 to facilitate fusion of Rab11- and L1-containing recycling vesicles. FRET-based biosensors showed that TC10 activity decreases at extending growth cones, and constitutively active TC10 could not rescue neurite outgrowth defects caused by TC10 depletion. FRET-based GTPase activity biosensors, TC10 knockdown, constitutively-active TC10 rescue, colocalization analysis, exocytosis assay in PC12 cells PloS one Medium 24223996
2007 NGF induces an interaction between activated TC10 and Exo70 in PC12 cells (detected by FRET/FLIM). The Exo70-TC10 complex locally antagonizes NGF-induced Cdc42-dependent activation of N-WASP at membrane protrusions. Exo70 is responsible for correct targeting of the complex to protrusion sites. FRET imaging by fluorescence lifetime microscopy (FLIM), dominant-negative mutants, siRNA knockdown, constitutively-active Cdc42 overexpression Journal of cell science Medium 17635999
2015 GIV/Girdin directly and constitutively binds Exo70 (exocyst subunit). Upon insulin stimulation, GIV associates with GLUT4-storage vesicles. Loss of GIV or its GEF function impairs membrane association of Exo70 and exocytosis of GLUT4 vesicles in response to insulin. In vitro direct binding assay, Co-immunoprecipitation, vesicle fractionation, GLUT4 exocytosis assay with GIV knockdown/GEF mutants Biochemical and biophysical research communications Medium 26514725
2020 In zebrafish, exoc7 loss-of-function causes microcephaly, demonstrating an essential role for EXOC7 in cerebral cortical development. In humans, partial loss-of-function variants in EXOC7 cause brain atrophy, seizures, and developmental delay. Zebrafish exoc7 knockout, in vitro EXOC7 splice-variant modeling, human genetic mapping (homozygosity mapping + exome sequencing) Genetics in medicine Medium 32103185
2010 EXO70 protein promotes dengue virus secretion/egression from infected cells. EXO70 knockdown significantly attenuates dengue virus production without affecting viral transcription or translation, indicating a specific role in virus exocytosis. siRNA knockdown, viral titer assay, viral RNA/protein quantification Microbes and infection Medium 21034848
2012 In C. elegans, exoc-7 (exo70) and exoc-8 (exo84) mutants show pleiotropic behavioral defects. exoc-8 and exoc-7;exoc-8 double mutations cause increased size of rab-10 RNAi-induced endocytic vacuoles in intestinal epithelia and affect RAB-10 expression and endocytic marker accumulation, linking Exo70 to RAB-10-dependent endosomal trafficking. C. elegans genetic mutant analysis, targeted RNAi screen for small GTPases, fluorescence microscopy of endocytic markers PloS one Medium 22389680
2018 In Drosophila, Exo70 is required for synaptic growth at the neuromuscular junction (NMJ). exo70 genetically interacts with the small GTPase ralA to regulate synaptic growth. Loss of Exo70 impairs integral membrane protein transport to the cell surface at synaptic terminals and blocks JNK signaling-, activity-, and temperature-induced synaptic outgrowths. Drosophila exo70 mutant alleles, genetic interaction (double mutant) with ralA, electrophysiology (mEPSP), immunofluorescence, membrane protein trafficking assays The Journal of neuroscience Medium 30209205
2024 Transglutaminases TGM1 and TGM3 transamidate Exo70 on Gln5 with Lys56 of cystatin A, promoting Exo70 association with other exocyst subunits and enhancing MMP secretion, invadopodia formation, and integrin delivery to the leading edge. Tumor suppressor LKB1 phosphorylates TGM1 (Thr386) and TGM3 (Thr282) to inhibit their interaction with Exo70 and block transamidation. The FDA-approved drug cantharidin inhibits Exo70 transamidation and suppresses tumor cell migration. Mass spectrometry identification of modification sites, site-directed mutagenesis, in vitro transamidation assay, kinase assay, Co-immunoprecipitation, invasion and invadopodia assays, in vivo tumor model Cell reports High 39146185
2021 Exo70 promotes cisplatin efflux from epithelial ovarian cancer cells through exocytosis, contributing to cisplatin resistance. Cisplatin-induced autophagy-lysosomal degradation of Exo70 is modulated by AMPK/mTOR phosphorylation. Knockdown of Exo70 or inhibition with ES2 reverses cisplatin resistance both in vitro and in vivo. Exo70 knockdown, ES2 inhibitor treatment, cisplatin efflux/uptake assay, autophagy pathway analysis (AMPK/mTOR phosphorylation), in vivo xenograft model Cancers Medium 34298686
2021 Exoc7/Exo70 opposes Prpf19/prp19 in regulating expanded ATXN3-polyQ protein toxicity in SCA3 models. Exoc7/exo70 modulates expanded ATXN3-polyQ levels by regulating the E3 ligase function of Prpf19, counteracting polyubiquitination and degradation of mutant ATXN3. Mammalian cell transfection, Drosophila disease model, ubiquitination assay, protein level analysis, genetic interaction Cell death & disease Medium 33542212
2021 Following mild traumatic brain injury in mice, Exo70 redistributes from the microsomal fraction into the synaptic compartment. Exocyst complex assembly and its interaction with GluN2B (NMDA receptor subunit) increase in the synaptic compartment after brain trauma. Subcellular fractionation, Co-immunoprecipitation, mouse repeated mTBI model Biological research Medium 33593425
2022 The brain-specific Cdc42b isoform (but not ubiquitous Cdc42u) interacts with Exo70 and regulates exocytosis of post-Golgi vesicles in the axonal growth cone to promote axon formation. Inactivation of Arhgef7 (activator of Cdc42b) or Cdc42b interferes with this exocytosis. Mammalian Cdc42u does not interact with Exo70. Co-immunoprecipitation of Cdc42 isoforms with Exo70, live-cell imaging of post-Golgi vesicle exocytosis, siRNA knockdown, dominant-negative expression Life science alliance Medium 36543541
2020 GIV/Girdin fulfills the function of yeast Bem1p as a polarity scaffold for Exo70 in mammalian cells; both bind Exo70 via similar short-linear interaction motifs and each prefers its evolutionary counterpart. Selective disruption of the GIV-Exo70 interaction blocks delivery of MT1-MMP to invadosomes and impairs collagen degradation. Co-immunoprecipitation, motif-based interaction assay, MT1-MMP trafficking assay, collagen degradation assay, haptotaxis assay iScience Medium 32590327
2019 Kinase suppressor of Ras 1 (KSR1) promotes fatty acid-stimulated neurotensin secretion via ERK1/2 signaling, which acts through Exo70. Inhibition of Exo70 potently inhibits basal and docosahexaenoic acid-stimulated neurotensin secretion from human endocrine cells, while Exo70 overexpression enhances it. Exo70 knockdown and overexpression, neurotensin secretion assay, ERK inhibitor treatment PloS one Low 30917119
2024 Exo70 redistributes from microsomes to synaptic compartment and increases interaction with GluN2B after mTBI. Exo70 overexpression in CA1 pyramidal neurons via lentiviral transduction prevented mTBI-induced cognitive impairment, preserved synaptic GluN2B-containing NMDARs, and maintained downstream signaling, suggesting Exo70 regulates NMDAR trafficking at synapses. Lentiviral Exo70 overexpression in vivo, Morris water maze, electrophysiology (synaptic transmission and LTP), GluN2B co-immunoprecipitation, mouse mTBI model Antioxidants Medium 40563275
2025 Cytoplasmic METTL3 interacts with EXOC7, promoting EXOC7 stabilization. METTL3 knockdown impairs vesicle trafficking and breast cancer secretome, impairs invadopodia formation and collagen invasion independently of METTL3's catalytic activity, implicating METTL3-mediated stabilization of EXOC7 as a non-catalytic mechanism. Co-immunoprecipitation of METTL3 and EXOC7, METTL3 knockdown, catalytic-dead METTL3 mutant rescue, vesicle trafficking assay, invasion assay bioRxivpreprint Low

Source papers

Stage 0 corpus · 68 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2007 Exo70 interacts with phospholipids and mediates the targeting of the exocyst to the plasma membrane. The EMBO journal 259 17717527
2004 Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p. The Journal of cell biology 242 15583031
1999 Rho3 of Saccharomyces cerevisiae, which regulates the actin cytoskeleton and exocytosis, is a GTPase which interacts with Myo2 and Exo70. Molecular and cellular biology 163 10207081
2006 Exo70 interacts with the Arp2/3 complex and regulates cell migration. Nature cell biology 148 17086175
2015 Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis. Proceedings of the National Academy of Sciences of the United States of America 125 26607451
2005 The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif. Nature structural & molecular biology 119 16249794
2015 Rice Exo70 interacts with a fungal effector, AVR-Pii, and is required for AVR-Pii-triggered immunity. The Plant journal : for cell and molecular biology 101 26186703
2009 The TC10-Exo70 complex is essential for membrane expansion and axonal specification in developing neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience 95 19846717
2013 Exo70 generates membrane curvature for morphogenesis and cell migration. Developmental cell 85 23948253
2005 Crystal structure of the S.cerevisiae exocyst component Exo70p. Journal of molecular biology 68 16359701
2012 ERK1/2 regulate exocytosis through direct phosphorylation of the exocyst component Exo70. Developmental cell 66 22595671
2013 Exo70 isoform switching upon epithelial-mesenchymal transition mediates cancer cell invasion. Developmental cell 62 24331928
2007 Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth. The Journal of cell biology 57 17339375
2012 Fission yeast Sec3 and Exo70 are transported on actin cables and localize the exocyst complex to cell poles. PloS one 55 22768263
2017 Analysis of Exocyst Subunit EXO70 Family Reveals Distinct Membrane Polar Domains in Tobacco Pollen Tubes. Plant physiology 51 28082718
2011 An association between type Iγ PI4P 5-kinase and Exo70 directs E-cadherin clustering and epithelial polarization. Molecular biology of the cell 49 22049025
2020 ULK1 phosphorylates Exo70 to suppress breast cancer metastasis. Nature communications 47 31913283
2013 GTP hydrolysis of TC10 promotes neurite outgrowth through exocytic fusion of Rab11- and L1-containing vesicles by releasing exocyst component Exo70. PloS one 46 24223996
2012 Exo70 stimulates the Arp2/3 complex for lamellipodia formation and directional cell migration. Current biology : CB 46 22748316
2005 Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70. Proceedings of the National Academy of Sciences of the United States of America 45 15705715
2007 Snapin interacts with the Exo70 subunit of the exocyst and modulates GLUT4 trafficking. The Journal of biological chemistry 40 17947242
2020 Three subfamilies of exocyst EXO70 family subunits in land plants: early divergence and ongoing functional specialization. Journal of experimental botany 38 31647563
2007 An NGF-induced Exo70-TC10 complex locally antagonises Cdc42-mediated activation of N-WASP to modulate neurite outgrowth. Journal of cell science 34 17635999
2009 Insulin regulates fusion of GLUT4 vesicles independent of Exo70-mediated tethering. The Journal of biological chemistry 29 19155211
2009 Exo70-mediated recruitment of nucleoporin Nup62 at the leading edge of migrating cells is required for cell migration. Traffic (Copenhagen, Denmark) 29 19552648
2022 A blast fungus zinc-finger fold effector binds to a hydrophobic pocket in host Exo70 proteins to modulate immune recognition in rice. Proceedings of the National Academy of Sciences of the United States of America 28 36252011
2019 Inducible Exoc7/Exo70 knockout reveals a critical role of the exocyst in insulin-regulated GLUT4 exocytosis. The Journal of biological chemistry 28 31740584
2017 The Physcomitrella patens exocyst subunit EXO70.3d has distinct roles in growth and development, and is essential for completion of the moss life cycle. The New phytologist 28 28397275
2008 An internal domain of Exo70p is required for actin-independent localization and mediates assembly of specific exocyst components. Molecular biology of the cell 27 18946089
2018 Identification and Characterization of the EXO70 Gene Family in Polyploid Wheat and Related Species. International journal of molecular sciences 26 30586859
2020 Regulation of human cerebral cortical development by EXOC7 and EXOC8, components of the exocyst complex, and roles in neural progenitor cell proliferation and survival. Genetics in medicine : official journal of the American College of Medical Genetics 24 32103185
2016 Expression and Functional Analysis of a Novel Group of Legume-specific WRKY and Exo70 Protein Variants from Soybean. Scientific reports 24 27572297
2014 Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p. The Journal of cell biology 24 25313406
2017 The role of Exo70 in exocytosis and beyond. Small GTPases 20 28489961
2022 A lineage-specific Exo70 is required for receptor kinase-mediated immunity in barley. Science advances 19 35857460
2011 Exo70, a subunit of the exocyst complex, interacts with SNEV(hPrp19/hPso4) and is involved in pre-mRNA splicing. The Biochemical journal 19 21639856
2010 EXO70 protein influences dengue virus secretion. Microbes and infection 19 21034848
2018 The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons. Molecular neurobiology 18 30374940
2024 Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. The Plant cell 17 38124479
2016 The role of Exo70 in vascular smooth muscle cell migration. Cellular & molecular biology letters 17 28536622
2016 Cortactin and Exo70 mediated invasion of hepatoma carcinoma cells by MMP-9 secretion. Molecular biology reports 16 27025610
2012 Exo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1. PloS one 16 23300727
2020 Appraising the Value of Serum and Serum-Derived Exosomal LncRNA-EXOC7 as a Promising Biomarker in Cervical Cancer. Clinical laboratory 15 32658426
2012 Exocyst subunits Exo70 and Exo84 cooperate with small GTPases to regulate behavior and endocytic trafficking in C. elegans. PloS one 14 22389680
2018 Drosophila Exo70 Is Essential for Neurite Extension and Survival under Thermal Stress. The Journal of neuroscience : the official journal of the Society for Neuroscience 13 30209205
2015 GIV/girdin binds exocyst subunit-Exo70 and regulates exocytosis of GLUT4 storage vesicles. Biochemical and biophysical research communications 13 26514725
2015 Identification and characterization of a novel group of legume-specific, Golgi apparatus-localized WRKY and Exo70 proteins from soybean. Journal of experimental botany 12 25805717
2021 A fine balance between Prpf19 and Exoc7 in achieving degradation of aggregated protein and suppression of cell death in spinocerebellar ataxia type 3. Cell death & disease 11 33542212
2021 Deregulation of Exo70 Facilitates Innate and Acquired Cisplatin Resistance in Epithelial Ovarian Cancer by Promoting Cisplatin Efflux. Cancers 11 34298686
2016 Exo70 is transcriptionally up-regulated by hepatic nuclear factor 4α and contributes to cell cycle control in hepatoma cells. Oncotarget 8 26848864
2021 Exo70 intracellular redistribution after repeated mild traumatic brain injury. Biological research 7 33593425
2021 Identification and Comprehensive Structural and Functional Analyses of the EXO70 Gene Family in Cotton. Genes 7 34680988
2019 Kinase suppressor of Ras 1 and Exo70 promote fatty acid-stimulated neurotensin secretion through ERK1/2 signaling. PloS one 7 30917119
2010 Different steps of sexual development are differentially regulated by the Sec8p and Exo70p exocyst subunits. FEMS microbiology letters 7 20180855
2024 Exo70 Promotes the Invasion of Pancreatic Cancer Cells via the Regulation of Exosomes. Cancers 6 38254825
2024 TGM1/3-mediated transamidation of Exo70 promotes tumor metastasis upon LKB1 inactivation. Cell reports 6 39146185
2021 Genome-wide identification and expression analysis of the EXO70 gene family in grape (Vitis vinifera L). PeerJ 6 33976971
2023 Genome-Wide Analysis of Exocyst Complex Subunit Exo70 Gene Family in Cucumber. International journal of molecular sciences 5 37446106
2022 An isoform-specific function of Cdc42 in regulating mammalian Exo70 during axon formation. Life science alliance 4 36543541
2024 LncRNA ZNF649-AS1 promotes trastuzumab resistance and TAM-dependent PD-L1 expression in breast cancer by regulating EXOC7 alternative splicing. Archives of biochemistry and biophysics 3 39159899
2025 ELABELA promotes the migration and homing of bone marrow mesenchymal stem cells to myocardial injury sites through the ERK1/2/miR-299a-5p/Exo70 pathway. Frontiers in pharmacology 2 39963243
2020 GIV/Girdin and Exo70 Collaboratively Regulate the Mammalian Polarized Exocytic Machinery. iScience 2 32590327
2024 Deletion of Exoc7, but not Exoc3, in male germ cells causes severe spermatogenesis failure with spermatocyte aggregation in mice. Experimental animals 1 38325858
2026 Genome-wide identification of the EXO70 genes to elucidate their potential roles for intraspecific cross-incompatibility in sweet potato (Ipomoea batatas L.). Frontiers in plant science 0 41835271
2026 Discovery of a novel dual-target modulator of ULK1 and ERK1/2 that suppresses triple-negative breast cancer progression and metastasis via the Exo70/Cav-1/MMPs axis. Acta pharmacologica Sinica 0 42219424
2026 Engineering an Exo70 integrated domain of a barley NLR for improved blast resistance. The Plant cell 0 42263189
2025 Exo70 Protects Against Memory and Synaptic Impairments Following Mild Traumatic Brain Injury. Antioxidants (Basel, Switzerland) 0 40563275
2024 TC10 differently controls the dynamics of Exo70 in growth cones of cortical and hippocampal neurons. Biophysical reports 0 39521348

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