Affinage

Showing KIF11 — EG5 is a alias.

KIF11

Kinesin-like protein KIF11 · UniProt P52732

Length
1056 aa
Mass
119.2 kDa
Annotated
2026-06-10
100 papers in source corpus 32 papers cited in narrative 32 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

KIF11/Eg5 is a slow, plus-end-directed kinesin-5 ATPase whose mechanochemical cycle is rate-limited by ATP-dependent microtubule dissociation (PMID:15247293), and which generates the antiparallel microtubule-sliding forces that drive centrosome separation, bipolar spindle assembly, and spindle elongation; in human cells it cooperates with PRC1-dependent KIF4A to slide midzone microtubules, and combined loss of the two motors abolishes chromosome segregation (PMID:33910056). Its essentiality for cell division is reflected in early embryonic lethality of homozygous knockout mice (PMID:18474226) and mitotic arrest with monoastral spindles upon loss of function (PMID:24370453). Targeting of Eg5 to the spindle and centrosomes is governed by a phosphorylation cascade: CDK1 (with CDK2 compensation) phosphorylates the conserved C-terminal bimC domain (Thr926/Thr927/Thr937), and Plk1 acting through Nek9-Nek6/7 adds Ser1033, both required for centrosome accumulation and separation (PMID:7753799, PMID:21642957, PMID:21522128); PP2A/B55α reverses Thr926 phosphorylation to permit mitotic exit (PMID:28487562). Eg5 force output is constrained by inhibitory partners TPX2, which slows the motor through microtubule and neck-region binding (PMID:26018074), and HMMR, which localizes TPX2 to promote inhibitory TPX2-Eg5 complexes (PMID:29386294). Eg5 abundance, motor activity, and spindle loading are further tuned by post-translational modifications: RNF20/40 monoubiquitination (PMID:27557628), NAT10 acetylation at K771 (PMID:35210604), HDAC1 deacetylation (PMID:28392145), and UFMylation at K564 (PMID:39085203), while Parkin represses Eg5 transcription via an Hsp70-JNK-c-Jun axis (PMID:18845538). Beyond mitosis, Eg5 transports β-actin mRNPs through direct ZBP1 (IGF2BP1) binding to support cell migration (PMID:25588836), localizes to basal bodies to restrain primary cilium length and disassembly (PMID:32811879), and in postmitotic neurons is phosphorylated by NEK7 to stabilize distal dendritic microtubules independently of motor activity (PMID:29899413) and acts as a brake on excitatory synaptic transmission (PMID:30479371).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1995 High

    Established that Eg5 spindle targeting is under cell-cycle control, defining the link between mitotic kinase activity and motor localization.

    Evidence Site-directed mutagenesis of the CDK1 phosphorylation site (T937) with immunofluorescence in Xenopus A6 cells

    PMID:7753799

    Open questions at the time
    • Did not identify the full set of regulatory kinases
    • Did not connect localization to force generation
  2. 2004 High

    Defined the mechanochemical cycle of the motor, showing microtubule dissociation rather than hydrolysis is rate-limiting and the mechanism resembles conventional kinesin.

    Evidence Pre-steady-state and steady-state kinetics with mantATP/mantADP on monomeric human Eg5

    PMID:15247293

    Open questions at the time
    • Used monomeric construct, not the native homotetramer
    • Did not address antiparallel sliding mechanics
  3. 2008 High

    Demonstrated absolute requirement of Eg5 for early mammalian development with no motor compensation, establishing biological essentiality.

    Evidence Knsl1 gene-targeted knockout mice with embryo analysis

    PMID:18474226

    Open questions at the time
    • Pre-implantation lethality precludes analysis of later tissue-specific roles
  4. 2008 Medium

    Revealed transcriptional repression of Eg5 by Parkin through an Hsp70-JNK-c-Jun route, distinguishing transcriptional control from proteasomal degradation.

    Evidence Promoter reporter, ubiquitination, and JNK/c-Jun phosphorylation assays with proteasome controls

    PMID:18845538

    Open questions at the time
    • Single lab
    • Physiological context of Parkin-Eg5 regulation not defined
  5. 2011 High

    Resolved the kinase hierarchy driving centrosome separation, placing CDK1/CDK2 and a Plk1-Nek9-Nek6/7 cascade upstream of distinct Eg5 phosphosites.

    Evidence In vitro kinase assays, phospho-specific antibodies, and centrosome separation epistasis (two companion EMBO studies)

    PMID:21522128 PMID:21642957

    Open questions at the time
    • Quantitative contribution of each phosphosite to motor activity not isolated
    • Did not reconstitute phosphorylation-dependent sliding in vitro
  6. 2010 High

    Identified Tiam1-Rac as an opposing prophase force, framing Eg5-driven separation as a balance of antagonistic forces.

    Evidence siRNA depletion, conditional Rac1 knockout, monastrol, and reciprocal rescue with live-cell imaging

    PMID:20346677

    Open questions at the time
    • Molecular target of the opposing force not pinpointed
    • Direct biochemical link to Eg5 not shown
  7. 2015 High

    Defined direct biophysical inhibition of the motor by TPX2 through dual microtubule and neck-region binding.

    Evidence TIRF single-molecule and gliding assays with truncated TPX2 constructs

    PMID:26018074

    Open questions at the time
    • In vivo regulatory consequence addressed only later
    • Structural basis of neck-region binding not resolved
  8. 2018 Medium

    Showed HMMR positions TPX2 to assemble inhibitory TPX2-Eg5 complexes, controlling spindle force balance and segregation fidelity.

    Evidence siRNA, Co-IP, and chemical epistasis with Eg5 inhibitor rescue and aneuploidy quantification

    PMID:29386294

    Open questions at the time
    • Single lab
    • Direct effect of HMMR on motor kinetics not measured
  9. 2021 High

    Directly measured Eg5's force-generating role in spindle elongation, showing it cooperates with KIF4A to slide midzone microtubules.

    Evidence Combined CRISPR/RNAi depletion with tubulin photoactivation, STED, and expansion microscopy

    PMID:33910056

    Open questions at the time
    • Relative force contribution of each motor not quantified
    • Regulation of the EG5-KIF4A cooperation unknown
  10. 2024 High

    Expanded the PTM regulatory layer, establishing acetylation, monoubiquitination, deacetylation, and UFMylation as controllers of Eg5 stability, activity, and spindle loading.

    Evidence Site-directed mutagenesis (K771Q/R, K564R), modification-specific antibodies, Co-IP, ubiquitination assays, and rescue in HeLa cells

    PMID:27557628 PMID:28392145 PMID:35210604 PMID:39085203

    Open questions at the time
    • Crosstalk between UFMylation and monoubiquitination not fully resolved
    • Temporal coordination of distinct PTMs during the cell cycle unclear
  11. 2015 High

    Uncovered a non-mitotic transport function, with Eg5 binding ZBP1 to deliver β-actin mRNPs and support cell migration.

    Evidence Reciprocal Co-IP, in vitro domain mapping, β-actin mRNA imaging, and migration assays

    PMID:25588836

    Open questions at the time
    • In vivo physiological relevance of mRNP transport not established
    • Coupling to motor stepping not biophysically dissected
  12. 2020 Medium

    Extended Eg5 function to interphase and postmitotic cells, including basal body regulation of cilia and dendritic microtubule stabilization, the latter motor-independent.

    Evidence siRNA, NEK7 kinase-dead and motor-dead rescues, live-cell microtubule imaging, cilia quantification (multiple studies)

    PMID:29899413 PMID:30479371 PMID:32811879

    Open questions at the time
    • Mechanism distinguishing motor-dependent vs motor-independent roles incompletely defined
    • Tissue-specific regulation of these functions unknown
  13. 2020 High

    Demonstrated tissue-selective dependence on Eg5-driven endothelial mitosis, most critical in rapidly growing CNS vascular beds and independent of β-catenin signaling.

    Evidence Endothelial-specific conditional Kif11 knockout with retinal flat-mount vasculature analysis

    PMID:31993640

    Open questions at the time
    • Basis of vascular-bed selectivity not explained
    • Downstream pathway driving the phenotype not identified

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the multiple PTMs, kinase cascades, and inhibitory partners are temporally integrated to tune Eg5 force output across mitotic stages and non-mitotic contexts remains unresolved.
  • No unified model linking PTM state to motor velocity in vivo
  • Mechanistic basis for switching between mitotic and post-mitotic functions unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003774 cytoskeletal motor activity 3 GO:0008092 cytoskeletal protein binding 2 GO:0140657 ATP-dependent activity 2
Localization
GO:0005815 microtubule organizing center 3 GO:0005856 cytoskeleton 2 GO:0005929 cilium 1
Pathway
R-HSA-112316 Neuronal System 3 R-HSA-1266738 Developmental Biology 3 R-HSA-1640170 Cell Cycle 3
Partners
HMMRNAT10NEK7PAK6PTENRNF20TPX2ZBP1

Evidence

Reading pass · 32 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 Phosphorylation of Eg5 at Thr937 (T937) by p34cdc2 (CDK1) is required for spindle localization: mutation of T937 to non-phosphorylatable alanine abolishes spindle localization, while T937S preserves it, establishing that cell-cycle-regulated phosphorylation at the conserved bimC C-terminal domain controls Eg5 targeting to the mitotic spindle. Transient transfection with myc-tagged Eg5 derivatives, site-directed mutagenesis, immunofluorescence localization in Xenopus A6 cells Proceedings of the National Academy of Sciences of the United States of America High 7753799
2004 Monomeric human Eg5 is a slow, plus-end-directed ATPase: mantATP binding is rapid (2–3 µM⁻¹s⁻¹), ATP hydrolysis is fast (~15 s⁻¹), but ATP-dependent microtubule dissociation is rate-limiting (~8 s⁻¹), making the ATPase mechanism more similar to conventional kinesin than to minus-end motors Ncd/Kar3. Sedimentation velocity, sedimentation equilibrium, analytical gel filtration, steady-state ATPase, stopped-flow kinetics with mantATP/mantADP The Journal of biological chemistry High 15247293
2006 Eg5 is downstream of and regulated by Bcr-Abl tyrosine kinase in Philadelphia chromosome-positive cells: imatinib treatment downregulates Eg5 expression in imatinib-sensitive but not imatinib-resistant (T315I) cells, placing Eg5 in the Bcr-Abl signaling axis. Western blot and RT-PCR after imatinib treatment of sensitive vs. resistant cell lines; antisense oligonucleotide knockdown; xenograft mouse model Cell cycle (Georgetown, Tex.) Medium 16969080
2006 Inhibition of Eg5 upregulates Hsp70 transcriptionally via a cis-regulatory element in the Hsp70 promoter through the PI3K/Akt pathway; this Hsp70 induction is cytoprotective and reduces sensitivity to Eg5 inhibitors, while blocking Hsp70 or PI3K/Akt increases apoptosis. Small-molecule inhibition of Eg5, antisense/siRNA knockdown of Hsp70, PI3K/Akt pathway inhibitors, promoter reporter assays, apoptosis assays in multiple myeloma cells The Journal of biological chemistry Medium 16627469
2008 Parkin E3 ubiquitin ligase represses Eg5 gene transcription (without triggering proteasomal degradation of Eg5 protein) by blocking c-Jun binding to the AP-1 site in the Eg5 promoter; Parkin achieves this through multiple monoubiquitination of Hsp70, which inactivates JNK and reduces c-Jun phosphorylation. Co-transfection, promoter reporter assays, ubiquitination assays, Western blot for JNK/c-Jun phosphorylation, proteasome inhibitor controls The Journal of biological chemistry Medium 18845538
2008 Homozygous Eg5 (Knsl1) knockout mice die during early embryogenesis prior to implantation, while heterozygotes are viable and fertile, demonstrating that Eg5 is essential for early mammalian development and cannot be compensated by another motor. Gene targeting in mice, embryo analysis Biochemical and biophysical research communications High 18474226
2010 Tiam1-Rac signaling at centrosomes during prophase antagonizes Eg5-driven centrosome separation: Tiam1-depleted cells or Rac1-deficient cells escape monastrol-induced (Eg5-inhibited) mitotic arrest, and Eg5 suppression in Tiam1-depleted cells rescues increased centrosome separation and chromosome congression errors, placing Tiam1-Rac as the first identified force opposing Eg5 in prophase. siRNA depletion, conditional Rac1 knockout in vivo, monastrol treatment, live-cell imaging, centrosome separation measurements, epistasis analysis Current biology : CB High 20346677
2011 Plk1 triggers centrosome separation in G2 phase through a two-step mechanism: CDK1 phosphorylates Eg5 first, then Plk1 activates Nek9, which through Nek6/7 phosphorylates Eg5 at Ser1033; both Ser1033 (Nek6/7 site) and Thr926 (CDK1 site) are required for Eg5 accumulation at centrosomes and subsequent centrosome separation. In vitro kinase assays, phospho-specific antibodies, siRNA knockdown, centrosome separation assays, epistasis analysis The EMBO journal High 21642957
2011 Plk1 controls Eg5 localization to centrosomes and drives centrosome separation independently of CDK1; CDK2 can compensate for CDK1 by phosphorylating Eg5 at Thr927; actin-dependent opposing forces slow Plk1-dependent separation in G2, while CDK1 triggers faster centrosome movement; microtubule stability modulates the rate of centrosome separation. Plk1 and CDK1 inhibitors, CDK2 knockdown, phospho-Eg5 antibodies, centrosome tracking, actin depolymerization, MT stabilization The EMBO journal High 21522128
2013 Eg5 restricts (acts as a brake on) anaphase B spindle elongation in mammalian cells: STLC (weakens Eg5-MT interaction) increases centrosome separation rate, while FCPT (rigor-like Eg5-MT interaction) decreases it; GFP-Eg5 accumulates in the spindle interzone during anaphase. Small-molecule perturbation (STLC, FCPT), live-cell centrosome tracking with GFP-γ-tubulin, fluorescence quantification of GFP-Eg5 Cytoskeleton (Hoboken, N.J.) Medium 24285623
2014 RNF20/40 ubiquitin ligase complex physically interacts with Eg5 during mitosis, monoubiquitinates Eg5, and stabilizes it; loss of RNF20/40 reduces Eg5 protein levels, causes spindle assembly defects, cell cycle arrest and apoptosis. Co-immunoprecipitation, ubiquitination assays, siRNA knockdown, spindle assembly analysis, in vivo xenograft models Nature communications High 27557628
2015 KIF11 directly binds ZBP1 (IGF2BP1) via defined regions on both proteins; this interaction is required for KIF11-mediated transport of β-actin mRNPs along microtubules; disruption of the KIF11-ZBP1 interaction in vivo delocalizes β-actin mRNA and impairs cell migration. Co-immunoprecipitation, pulldown, in vitro binding domain mapping, β-actin mRNA localization (FISH/imaging), cell migration assays Journal of cell science High 25588836
2015 TPX2 inhibits Eg5 velocity through two mechanisms: binding to microtubules (Kd ~200 nM, C-terminal tubulin tails not required) and direct interaction with the dimerization/neck region of Eg5; dimeric but not monomeric Eg5 is differentially inhibited by full-length vs. truncated TPX2. TIRF microscopy single-molecule assays, microtubule gliding assays, pulldown/binding assays with truncated TPX2 The Journal of biological chemistry High 26018074
2016 PTEN associates and co-localizes with EG5 during mitosis; PTEN deficiency induces aberrant EG5 phosphorylation and abrogates EG5 recruitment to the mitotic spindle, leading to shorter spindles and chromosome misalignment. Co-immunoprecipitation, immunofluorescence co-localization, PTEN knockdown/knockout, spindle geometry measurement, chromosome alignment analysis Nature communications Medium 27492783
2017 HDAC1 deacetylates Eg5 and co-localizes with Eg5 during mitosis; HDAC1 influences Eg5 ATPase activity; an HDAC1/2-selective inhibitor causes mitotic arrest and monopolar spindle formation consistent with Eg5 hyperacetylation impairing function, revealing a non-transcriptional mechanism of HDAC inhibitor-induced G2/M arrest. HDAC1 trapping mutant substrate identification, co-localization, ATPase activity assay, cell cycle analysis, spindle morphology Cell chemical biology Medium 28392145
2017 PP2A/B55α complex physically associates with the Eg5 C-terminal tail domain and dephosphorylates Eg5 at Thr926, enabling mitotic exit; PP2A knockdown leads to elevated phospho-Eg5 in late metaphase and delayed mitotic exit, phenocopied by a phosphomimetic EG5/T926D mutant. Co-immunoprecipitation, phospho-specific antibodies, siRNA knockdown, phosphomimetic/non-phosphorylatable mutants, mitotic timing assays Scientific reports Medium 28487562
2018 NEK7 phosphorylates Eg5/KIF11, promoting its accumulation on microtubules in distal dendrites; there Eg5 limits retrograde microtubule polymerization through microtubule stabilization (independent of its motor activity), controlling dendrite growth and branching in postmitotic neurons. Targeted RNAi, kinase-dead NEK7 rescue, motor-dead Eg5 rescue, live-cell microtubule dynamics imaging, in vivo dendritic morphology analysis Nature communications High 29899413
2018 HMMR is required to dampen Eg5-mediated forces by localizing TPX2 and promoting formation of inhibitory TPX2-Eg5 complexes; HMMR silencing causes spindle disorganization, reduced K-fiber stability, and increased chromosome segregation errors that are rescued by chemical inhibition of Eg5 but not by KIF15 silencing. siRNA knockdown, immunofluorescence, Co-IP, Eg5 inhibitor rescue experiments, aneuploidy quantification Molecular biology of the cell Medium 29386294
2018 KIF11 knockdown in hippocampal neurons increases excitatory post-synaptic current frequency and amplitude, enhances dendritic arborization and synapse number, and upregulates synaptic vesicle proteins; KIF11 requires pre-synaptic NMDAR activity for its constraining function, and Piccolo expression constrains KIF11 function in synaptic transmission. RNAi-mediated knockdown in primary hippocampal neurons, electrophysiology (EPSC recording), immunofluorescence for synaptic proteins Scientific reports Medium 30479371
2018 KIF11 interacts with death receptor 6 (DR6) and TRAF4 by co-immunoprecipitation and GST pulldown; overexpression of KIF11 or TRAF4 rescues the suppression of ovarian cancer cell migration caused by DR6 knockdown, placing KIF11 downstream of DR6/TRAF4 in migration signaling. Co-immunoprecipitation, GST pulldown, mass spectrometry, rescue overexpression experiments, migration assays FEBS open bio Medium 30186750
2020 KIF11 localizes to basal bodies of primary cilia during interphase (in addition to its mitotic role); reduction of KIF11 expression increases the number of ciliated cells, increases cilium length, and attenuates cilia disassembly kinetics; exogenous KIF11 rescues these effects. Immunofluorescence localization, siRNA knockdown, cilia number/length quantification, rescue with exogenous KIF11 Scientific reports Medium 32811879
2020 TRIM8 E3 ubiquitin ligase interacts with KIF11/Eg5 (identified by proteomics in neural stem cells) and KIFC1; TRIM8 localizes to the mitotic spindle and plays a role in centrosome separation at mitosis onset, with consequent effects on mitotic progression and chromosomal stability. Co-immunoprecipitation, mass spectrometry interactome, immunofluorescence, TRIM8 knockdown, mitotic phenotype analysis Cancer letters Medium 31904480
2021 EG5/kinesin-5 together with PRC1-dependent KIF4A/kinesin-4 constitute the force-generating mechanism for spindle elongation in human cells: combined depletion of EG5 and KIF4A causes total failure of chromosome segregation due to blocked midzone microtubule sliding without affecting microtubule stability, as shown by tubulin photoactivation, STED, and expansion microscopy. Combined siRNA depletion, CRISPR inactivation, tubulin photoactivation, STED microscopy, expansion microscopy, live-cell imaging Developmental cell High 33910056
2022 NAT10 acetyltransferase binds and co-localizes with Eg5 at the centrosome during mitosis, acetylates Eg5 at K771, and stabilizes Eg5; K771 acetylation is required for Eg5 motor function and centrosome loading; a K771Q (acetylation-mimic) but not K771R mutant rescues NAT10-depletion-induced spindle defects and mitotic catastrophe. Co-immunoprecipitation, acetylation-specific antibody generation, site-directed mutagenesis (K771Q/R), live-cell imaging, centrosome loading assay, spindle formation rescue Cell death and differentiation High 35210604
2022 KIF11 interacts with SREBP2, the master regulator of the mevalonate pathway; KIF11 attenuates ubiquitination-mediated degradation of SREBP2, increasing its protein stability and accumulation, which in turn elevates mevalonate pathway gene expression and free cholesterol in PDAC cells. Co-immunoprecipitation, ubiquitination assays, Western blot for SREBP2 stability, GSEA pathway analysis, rescue experiments with statin treatment Cancer medicine Medium 35619540
2024 UFMylation of Eg5 at Lys564 is required for proper spindle organization: Eg5 is a substrate of the UFM1 pathway; UFMylation does not alter Eg5 transcription, phosphorylation, or protein stability but affects its mono-ubiquitination and spindle localization; the K564R (UFMylation-defective) mutant shows shorter spindles, metaphase arrest, spindle checkpoint activation, and cell division failure. Identification of Eg5 as UFM1 substrate, site-directed mutagenesis (K564R), co-localization at centrosome/spindle, ubiquitination assays, spindle length and checkpoint assays in HeLa cells Cell death & disease Medium 39085203
1998 Eg5 is expressed in postmitotic rodent neurons, directly associates with the microtubule array, and is enriched in distal regions of developing axons and dendrites transiently during process outgrowth, re-appearing in newly forming dendritic sprouts, suggesting a role in regulating microtubule behavior at distal tips of developing neuronal processes. Western blot, immunofluorescence, microtubule co-pelleting from egg homogenates The Journal of neuroscience Medium 9742151
2014 Inhibition of kinesin-5 (Eg5) by amyloid-beta (Aβ) or monastrol reduces transport of NGF/NTR(p75) and NMDA receptors to the cell surface, inhibits NGF-dependent neurite outgrowth from PC12 cells, blocks glutamate-dependent Ca²⁺ entry into primary neurons, and inhibits long-term potentiation; Eg5 activity is absent from APP/PS transgenic mouse brain. Cell-surface receptor quantification, neurite outgrowth assay, Ca²⁺ imaging, LTP electrophysiology, monastrol and Aβ treatment Neurobiology of aging Medium 24636920
2020 In mice, postnatal EC-specific conditional knockout of Kif11 leads to severely stunted growth of the retinal vasculature and mildly stunted cerebellar vasculature, with little effect elsewhere in the CNS, demonstrating Kif11 function in endothelial cell mitosis is most critical in rapidly growing CNS vascular beds; this phenotype is not caused by reduced β-catenin signaling in ECs. Conditional knockout mouse (Kif11 CKO in endothelial cells), retinal flat-mount vasculature analysis, β-catenin signaling readouts Human molecular genetics High 31993640
2021 ASPM physically interacts with KIF11 (by co-immunoprecipitation in HCC cells), and ASPM exerts its effects on HCC cell proliferation, invasion, and migration through KIF11; the ASPM-KIF11 complex promotes malignant progression by activating the Wnt/β-catenin signaling pathway. Co-immunoprecipitation, rescue overexpression of KIF11 after ASPM knockdown, Western blot for Wnt/β-catenin components Experimental and therapeutic medicine Low 34504599
2020 PAK6 specifically co-localizes with Eg5 at the centrosome; PAK6 depletion results in multipolar spindles and upregulation of Eg5; Eg5 knockdown reverses PAK6-depletion-induced multipolar spindles; PAK6 and Eg5 negatively inter-regulate each other's expression. Co-localization by immunofluorescence, siRNA knockdown epistasis, Western blot for protein levels Biochimica et biophysica acta. Molecular cell research Low 33098954
2013 Loss of Kif11 function in zebrafish radial glial cells (by mutation or STLC inhibition) causes monoastral spindle formation and mitotic arrest (~226× delay in mitotic exit), leading to apoptosis and specific reductions in oligodendroglia and secondary interneurons/motorneurons, demonstrating Kif11 is required for radial glia mitotic progression and subsequent neurogenesis. Forward genetic screen, pharmacological inhibition (STLC), anti-activated Caspase-3 staining, mathematical modeling, cell-type-specific marker analysis Developmental biology Medium 24370453

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1995 Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle. Proceedings of the National Academy of Sciences of the United States of America 243 7753799
2011 Nek9 is a Plk1-activated kinase that controls early centrosome separation through Nek6/7 and Eg5. The EMBO journal 150 21642957
2015 The mitotic kinesin KIF11 is a driver of invasion, proliferation, and self-renewal in glioblastoma. Science translational medicine 136 26355032
2018 Circular RNA‑MTO1 suppresses breast cancer cell viability and reverses monastrol resistance through regulating the TRAF4/Eg5 axis. International journal of oncology 113 30015883
2014 Phenotypic overlap between familial exudative vitreoretinopathy and microcephaly, lymphedema, and chorioretinal dysplasia caused by KIF11 mutations. JAMA ophthalmology 113 25124931
2020 Eg5 targeting agents: From new anti-mitotic based inhibitor discovery to cancer therapy and resistance. Biochemical pharmacology 89 33310050
2011 Differential control of Eg5-dependent centrosome separation by Plk1 and Cdk1. The EMBO journal 87 21522128
2004 Mechanistic analysis of the mitotic kinesin Eg5. The Journal of biological chemistry 78 15247293
2010 Ectopic expression of the microtubule-dependent motor protein Eg5 promotes pancreatic tumourigenesis. The Journal of pathology 77 20455257
1998 Expression of the mitotic motor protein Eg5 in postmitotic neurons: implications for neuronal development. The Journal of neuroscience : the official journal of the Society for Neuroscience 76 9742151
2013 Microcephaly with or without chorioretinopathy, lymphoedema, or mental retardation (MCLMR): review of phenotype associated with KIF11 mutations. European journal of human genetics : EJHG 71 24281367
2015 Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility. Journal of cell science 67 25588836
2015 KIF11 mutations are a common cause of autosomal dominant familial exudative vitreoretinopathy. The British journal of ophthalmology 66 26472404
2016 Ubiquitin ligase RNF20/40 facilitates spindle assembly and promotes breast carcinogenesis through stabilizing motor protein Eg5. Nature communications 60 27557628
2018 KIF11 Functions as an Oncogene and Is Associated with Poor Outcomes from Breast Cancer. Cancer research and treatment 58 30590004
2021 Microtubule-sliding modules based on kinesins EG5 and PRC1-dependent KIF4A drive human spindle elongation. Developmental cell 56 33910056
2022 NAT10 regulates mitotic cell fate by acetylating Eg5 to control bipolar spindle assembly and chromosome segregation. Cell death and differentiation 54 35210604
2013 Impaired angiogenesis and tumor development by inhibition of the mitotic kinesin Eg5. Oncotarget 54 24327603
2021 KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer. International journal of biological sciences 52 33613109
2007 Polymorphisms in the IDE-KIF11-HHEX gene locus are reproducibly associated with type 2 diabetes in a Japanese population. The Journal of clinical endocrinology and metabolism 52 17971426
2006 Eg5 steps it up! Cell division 51 17173688
1994 The kinesin-related protein Eg5 associates with both interphase and spindle microtubules during Xenopus early development. Developmental biology 50 8026619
2010 Tiam1-Rac signaling counteracts Eg5 during bipolar spindle assembly to facilitate chromosome congression. Current biology : CB 48 20346677
2006 Eg5 expression is closely correlated with the response of advanced non-small cell lung cancer to antimitotic agents combined with platinum chemotherapy. Lung cancer (Amsterdam, Netherlands) 48 16934364
2016 PTEN regulates EG5 to control spindle architecture and chromosome congression during mitosis. Nature communications 44 27492783
2019 Identification of KIF11 As a Novel Target in Meningioma. Cancers 43 30991738
2014 Congenital microcephaly and chorioretinopathy due to de novo heterozygous KIF11 mutations: five novel mutations and review of the literature. American journal of medical genetics. Part A 43 25115524
2023 LncRNA NEAT1 suppresses cellular senescence in hepatocellular carcinoma via KIF11-dependent repression of CDKN2A. Clinical and translational medicine 42 37752791
2006 Inhibition of the mitotic kinesin Eg5 up-regulates Hsp70 through the phosphatidylinositol 3-kinase/Akt pathway in multiple myeloma cells. The Journal of biological chemistry 42 16627469
2006 Regulation and targeting of Eg5, a mitotic motor protein in blast crisis CML: overcoming imatinib resistance. Cell cycle (Georgetown, Tex.) 42 16969080
2016 Overexpression of KIF11 in Gastric Cancer with Intestinal Mucin Phenotype. Pathobiology : journal of immunopathology, molecular and cellular biology 39 27459100
2008 Disruption of the mitotic kinesin Eg5 gene (Knsl1) results in early embryonic lethality. Biochemical and biophysical research communications 39 18474226
2017 KIF11 is required for proliferation and self-renewal of docetaxel resistant triple negative breast cancer cells. Oncotarget 38 29190901
2014 Alzheimer amyloid beta inhibition of Eg5/kinesin 5 reduces neurotrophin and/or transmitter receptor function. Neurobiology of aging 38 24636920
2008 Parkin regulates Eg5 expression by Hsp70 ubiquitination-dependent inactivation of c-Jun NH2-terminal kinase. The Journal of biological chemistry 38 18845538
2018 NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization. Nature communications 37 29899413
2017 HDAC Inhibitor-Induced Mitotic Arrest Is Mediated by Eg5/KIF11 Acetylation. Cell chemical biology 37 28392145
2017 KIF11 silencing and inhibition induces chromosome instability that may contribute to cancer. Genes, chromosomes & cancer 36 28510357
2008 Effects of Eg5 knockdown on human prostate cancer xenograft growth and chemosensitivity. The Prostate 36 18512732
2014 Synergism between inhibitors of Aurora A and KIF11 overcomes KIF15-dependent drug resistance. Molecular oncology 35 24950801
2013 The expression of Eg5 predicts a poor outcome for patients with renal cell carcinoma. Medical oncology (Northwood, London, England) 33 23371254
2021 KIF11 Promotes Proliferation of Hepatocellular Carcinoma among Patients with Liver Cancers. BioMed research international 32 33490265
2016 Aurora A, MCAK, and Kif18b promote Eg5-independent spindle formation. Chromosoma 32 27354041
2015 TPX2 Inhibits Eg5 by Interactions with Both Motor and Microtubule. The Journal of biological chemistry 32 26018074
2013 Eg5 restricts anaphase B spindle elongation in mammalian cells. Cytoskeleton (Hoboken, N.J.) 32 24285623
2024 Mitotic Functions and Characters of KIF11 in Cancers. Biomolecules 31 38672404
2020 A mouse model for kinesin family member 11 (Kif11)-associated familial exudative vitreoretinopathy. Human molecular genetics 30 31993640
2017 KIF11 Is Required for Spheroid Formation by Oesophageal and Colorectal Cancer Cells. Anticancer research 30 28011472
2016 Kinesin family members KIF11 and KIF23 as potential therapeutic targets in malignant pleural mesothelioma. International journal of oncology 30 27279560
2015 A Potent Chemotherapeutic Strategy with Eg5 Inhibitor against Gemcitabine Resistant Bladder Cancer. PloS one 28 26658059
2018 Kinesin Family of Proteins Kif11 and Kif21B Act as Inhibitory Constraints of Excitatory Synaptic Transmission Through Distinct Mechanisms. Scientific reports 27 30479371
2009 KIF11 inhibition for glioblastoma treatment: reason to hope or a struggle with the brain? BMC cancer 27 19545421
2021 Tozasertib Attenuates Neuropathic Pain by Interfering with Aurora Kinase and KIF11 Mediated Nociception. ACS chemical neuroscience 26 34027667
2020 Enhancing Brain Retention of a KIF11 Inhibitor Significantly Improves its Efficacy in a Mouse Model of Glioblastoma. Scientific reports 26 32300151
2012 Receptor-ligand interaction-based virtual screening for novel Eg5/kinesin spindle protein inhibitors. Journal of medicinal chemistry 26 22309208
2021 KIF11 inhibitors filanesib and ispinesib inhibit meningioma growth in vitro and in vivo. Cancer letters 25 33652084
2022 KIF11 As a Potential Pan-Cancer Immunological Biomarker Encompassing the Disease Staging, Prognoses, Tumor Microenvironment, and Therapeutic Responses. Oxidative medicine and cellular longevity 24 36742345
2022 KIF11 manipulates SREBP2-dependent mevalonate cross talk to promote tumor progression in pancreatic ductal adenocarcinoma. Cancer medicine 23 35619540
2020 TRIM8 interacts with KIF11 and KIFC1 and controls bipolar spindle formation and chromosomal stability. Cancer letters 23 31904480
2018 Death receptor 6 promotes ovarian cancer cell migration through KIF11. FEBS open bio 22 30186750
2014 Nuclear Eg5 (kinesin spindle protein) expression predicts docetaxel response and prostate cancer aggressiveness. Oncotarget 22 25277178
2017 Biochemical and Biophysical characterization of curcumin binding to human mitotic kinesin Eg5: Insights into the inhibitory mechanism of curcumin on Eg5. International journal of biological macromolecules 21 29162464
2022 Update on the Phenotypic and Genotypic Spectrum of KIF11-Related Retinopathy. Genes 20 35456519
2018 The nonmotor adaptor HMMR dampens Eg5-mediated forces to preserve the kinetics and integrity of chromosome segregation. Molecular biology of the cell 20 29386294
2013 Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube. Developmental biology 20 24370453
2012 p63 expression correlates with sensitivity to the Eg5 inhibitor ZD4877 in bladder cancer cells. Cancer biology & therapy 20 22361733
2020 p21-activated kinase 6 controls mitosis and hepatocellular carcinoma progression by regulating Eg5. Biochimica et biophysica acta. Molecular cell research 19 33098954
2018 Non-canonical functions of the mitotic kinesin Eg5. Thoracic cancer 19 29927078
2012 Inhibition of Eg5 acts synergistically with checkpoint abrogation in promoting mitotic catastrophe. Molecular cancer research : MCR 19 22522457
2022 LncRNA VPS9D1-AS1 Promotes Malignant Progression of Lung Adenocarcinoma by Targeting miRNA-30a-5p/KIF11 Axis. Frontiers in genetics 18 35140744
2022 Using a comprehensive approach to investigate the interaction between Kinesin-5/Eg5 and the microtubule. Computational and structural biotechnology journal 18 36051882
2021 Upregulation of KIF11 in TP53 Mutant Glioma Promotes Tumor Stemness and Drug Resistance. Cellular and molecular neurobiology 18 33491154
2021 KIF11 is upregulated in colorectal cancer and silencing of it impairs tumor growth and sensitizes colorectal cancer cells to oxaliplatin via p53/GSK3β signaling. Journal of Cancer 18 33995648
2019 Kinesin Eg5 Targeting Inhibitors as a New Strategy for Gastric Adenocarcinoma Treatment. Molecules (Basel, Switzerland) 18 31683688
2023 In vitro antitumor activity, molecular dynamics simulation, DFT study, ADME prediction, and Eg5 binding of enastron analogues. RSC advances 17 37388149
2021 ASPM combined with KIF11 promotes the malignant progression of hepatocellular carcinoma via the Wnt/β-catenin signaling pathway. Experimental and therapeutic medicine 17 34504599
2019 MicroRNA-186-5p represses neuroblastoma cell growth via downregulation of Eg5. American journal of translational research 17 31105832
2018 Meiotic arrest and spindle defects are associated with altered KIF11 expression in porcine oocytes. Environmental and molecular mutagenesis 17 30151839
2014 Knockdown of kinesin KIF11 abrogates directed migration in response to epidermal growth factor-mediated chemotaxis. Biochemical and biophysical research communications 17 25193695
2025 Identification and validation of the important role of KIF11 in the development and progression of endometrial cancer. Journal of translational medicine 16 39806429
2024 Di-(2-ethylhexyl) phthalate promotes benign prostatic hyperplasia through KIF11-Wnt/β-catenin signaling pathway. Ecotoxicology and environmental safety 16 38944010
2024 Eg5 UFMylation promotes spindle organization during mitosis. Cell death & disease 16 39085203
2022 Kinesin-5 Eg5 is essential for spindle assembly, chromosome stability and organogenesis in development. Cell death discovery 16 36513626
2021 High KIF11 expression is associated with poor outcome of NSCLC. Tumori 16 33554761
2021 Retinal Features of Family Members With Familial Exudative Vitreoretinopathy Caused By Mutations in KIF11 Gene. Translational vision science & technology 16 34128965
2020 An interphase pool of KIF11 localizes at the basal bodies of primary cilia and a reduction in KIF11 expression alters cilia dynamics. Scientific reports 16 32811879
2017 Protein Phosphatase 2A (PP2A) Regulates EG5 to Control Mitotic Progression. Scientific reports 16 28487562
2016 In Vitro Maturation of Mouse Oocytes Increases the Level of Kif11/Eg5 on Meiosis II Spindles. Biology of reproduction 16 27146033
2008 Cloning, enzyme characterization of recombinant human Eg5 and the development of a new inhibitor. Biological & pharmaceutical bulletin 16 18591782
2023 QSAR-based virtual screening of traditional Chinese medicine for the identification of mitotic kinesin Eg5 inhibitors. Computational biology and chemistry 15 37062146
2018 S-trityl-L-cysteine, a novel Eg5 inhibitor, is a potent chemotherapeutic strategy in neuroblastoma. Oncology letters 15 29963178
2017 Eg5 inhibitor YL001 induces mitotic arrest and inhibits tumor proliferation. Oncotarget 15 28489567
2023 Cyclovirobuxine D inhibits growth and progression of non‑small cell lung cancer cells by suppressing the KIF11‑CDC25C‑CDK1‑CyclinB1 G2/M phase transition regulatory network and the NFκB/JNK signaling pathway. International journal of oncology 14 36929198
2023 Morphological Profiling Identifies the Motor Protein Eg5 as Cellular Target of Spirooxindoles. Angewandte Chemie (International ed. in English) 14 36929571
2021 KIF11, a plus end-directed kinesin, as a key gene in benzo(a)pyrene-induced non-small cell lung cancer. Environmental toxicology and pharmacology 14 34800719
2020 Kinesin-5 Eg5 is essential for spindle assembly and chromosome alignment of mouse spermatocytes. Cell division 14 32165913
2020 KIF11 and KIF15 mitotic kinesins are potential therapeutic vulnerabilities for malignant peripheral nerve sheath tumors. Neuro-oncology advances 14 32642733
2019 KIF11 as a Potential Marker of Spermatogenesis Within Mouse Seminiferous Tubule Cross-sections. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 14 31424977
2018 KIF11 microdeletion is associated with microcephaly, chorioretinopathy and intellectual disability. Human genome variation 14 31428438
2017 CD52, CD22, CD26, EG5 and IGF-1R expression in thymic malignancies. Lung cancer (Amsterdam, Netherlands) 14 28625631

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