- Length
- 186 aa
- Mass
- 21.1 kDa
- Annotated
- 2026-04-28
47 papers in source corpus
12 papers cited in narrative
10 extracted findings
Mechanistic narrative
Synthesis pass · prose summary of the discoveries below
Parse failed — see logs
Mechanism profile
Synthesis pass · controlled-vocabulary classification · explore literature graph →
No controlled-vocabulary terms were assigned to this entry.
Evidence
Reading pass · 10 per-paper findings extracted from the source corpus
| Year | Finding | Method | Journal | Conf | PMIDs |
|---|---|---|---|---|---|
| 2013 | FMDV nonstructural protein 3A directly binds host DCTN3 (a dynactin complex subunit) as identified by yeast two-hybrid and confirmed by co-immunoprecipitation in mammalian cells. Overexpression of DCTN3, or of dynein-disrupting proteins p150/Glued and dynamitin, decreased FMDV replication. Mutational mapping identified the critical 3A residues mediating the 3A-DCTN3 interaction, and a FMDV mutant unable to bind DCTN3 showed delayed disease in cattle, demonstrating that the 3A-DCTN3 interaction contributes to viral virulence and replication. | Yeast two-hybrid, co-immunoprecipitation in mammalian cells, overexpression assay, mutational analysis, in vivo cattle infection model | Journal of virology | High | 24352458 |
| 2013 | DCTN3 (light subunit of the dynactin complex) protein and mRNA levels are decreased in the spinal cord of sporadic ALS patients and in SOD1(G93A) mice at both presymptomatic and symptomatic stages, supporting a role for DCTN3 in dynein/dynactin complex integrity required for retrograde axonal transport in motor neurons. | Western blot, qRT-PCR, immunohistochemistry in human ALS tissue and transgenic mouse models | Neurochemical research | Medium | 24078265 |
| 2021 | shRNA-mediated knockdown of DCTN3 (dynactin subunit) in human neuroblastoma SK-N-SH cells inhibited intracellular transport of HSV-1 virion capsids toward the nucleus, establishing a required role for DCTN3 in dynein-dependent retrograde capsid transport during viral infection. | shRNA knockdown via lentivirus, live-cell imaging/tracking of virion capsids in neuroblastoma cells | Journal of virology | Medium | 33472938 |
| 2014 | In vitro silencing of DCTN3 in oral cancer cell lines reduced cell proliferation and anchorage-independent growth, while activation experiments increased these phenotypes, identifying DCTN3 as a candidate oncogene activated by DNA copy number gain at chromosome 9p13 during oral cancer progression. | siRNA silencing, overexpression, proliferation assay, soft-agar anchorage-independent growth assay in oral cancer cell lines | Cancer medicine | Medium | 25060540 |
| 2015 | DCTN3 (dynactin subunit) expression changes dramatically during fertilization in human oocytes. Overexpression of Dctn3 in oocytes suppressed Plk1 expression, suggesting a regulatory relationship between DCTN3 and Plk1 in early embryonic development. Aberrant levels of maternal Dctn3 in in-vitro-matured oocytes were associated with increased embryo aneuploidy and developmental failure. | Differential gene expression analysis of human oocytes vs. zygotes, Dctn3 overexpression in oocytes with Plk1 readout, comparison of in-vivo vs. in-vitro matured oocytes | Scientific reports | Medium | 25645239 |
| 2015 | Large-scale AP-MS (BioPlex) in HEK293T cells identified DCTN3 as a component of the human dynactin complex interactome, detecting its interactions with other dynactin subunits (DCTN1, DCTN2, ACTR1A/Arp1, etc.) within the broader human protein interaction network. | High-throughput affinity purification-mass spectrometry (AP-MS) in HEK293T cells | Cell | Medium | 26186194 28514442 33961781 |
| 2017 | BioID proximity biotinylation attached to dynactin subunit DCTN1 (p150) detected DCTN3 as a proximal interactor within the dynein/dynactin machinery in living human cells, placing DCTN3 spatially within the dynactin complex in the cytoplasm. | BioID proximity-dependent biotinylation coupled to mass spectrometry in human cells | eLife | Medium | 28718761 |
| 2021 | OpenCell endogenous tagging of DCTN3 via CRISPR in human cells combined with confocal live-cell imaging and AP-MS defined DCTN3 localization to the cytoplasm/microtubule cytoskeleton and confirmed its interactions with other dynactin complex members. | CRISPR endogenous GFP tagging, confocal live-cell imaging, AP-MS interactome profiling | Science (New York, N.Y.) | Medium | 35271311 |
| 2012 | Biochemical fractionation of human cell extracts followed by quantitative MS identified DCTN3 as a stably associated component of the human dynactin complex, one of 622 high-confidence protein complexes detected in this proteome-wide survey. | Size-exclusion chromatography fractionation of human cell lysates coupled with quantitative tandem mass spectrometry | Cell | Medium | 22939629 |
| 2015 | Cross-linking mass spectrometry of HeLa cell lysates identified structural cross-links involving DCTN3 within the dynactin complex, providing distance constraints that report on the physical organization of DCTN3 relative to other dynactin subunits in the intact complex. | MS-cleavable cross-linking mass spectrometry (XlinkX) in HeLa cell lysates | Nature methods | Medium | 26414014 |
Source papers
Stage 0 corpus · 47 papers · ranked by NIH iCite citations
| Year | Title | Journal | Citations | PMID |
|---|---|---|---|---|
| 2002 | Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. | Proceedings of the National Academy of Sciences of the United States of America | 1479 | 12477932 |
| 2015 | The BioPlex Network: A Systematic Exploration of the Human Interactome. | Cell | 1118 | 26186194 |
| 2017 | Architecture of the human interactome defines protein communities and disease networks. | Nature | 1085 | 28514442 |
| 2015 | A human interactome in three quantitative dimensions organized by stoichiometries and abundances. | Cell | 1015 | 26496610 |
| 2020 | A reference map of the human binary protein interactome. | Nature | 849 | 32296183 |
| 2003 | Complete sequencing and characterization of 21,243 full-length human cDNAs. | Nature genetics | 754 | 14702039 |
| 2021 | Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. | Cell | 705 | 33961781 |
| 2012 | A census of human soluble protein complexes. | Cell | 689 | 22939629 |
| 2011 | Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. | Briefings in bioinformatics | 656 | 21873635 |
| 2006 | A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. | Cell | 610 | 16713569 |
| 2004 | The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). | Genome research | 438 | 15489334 |
| 2015 | A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. | Cell | 433 | 26638075 |
| 2022 | OpenCell: Endogenous tagging for the cartography of human cellular organization. | Science (New York, N.Y.) | 432 | 35271311 |
| 1995 | Cell cycle regulation of the activity and subcellular localization of Plk1, a human protein kinase implicated in mitotic spindle function. | The Journal of cell biology | 427 | 7790358 |
| 2010 | Systematic analysis of human protein complexes identifies chromosome segregation proteins. | Science (New York, N.Y.) | 421 | 20360068 |
| 2005 | Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. | Genome research | 409 | 16344560 |
| 2015 | Proteome-wide profiling of protein assemblies by cross-linking mass spectrometry. | Nature methods | 370 | 26414014 |
| 2021 | A proximity-dependent biotinylation map of a human cell. | Nature | 339 | 34079125 |
| 2012 | A high-throughput approach for measuring temporal changes in the interactome. | Nature methods | 273 | 22863883 |
| 2004 | Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. | Nature biotechnology | 266 | 15146197 |
| 2011 | Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods. | The EMBO journal | 265 | 21399614 |
| 2011 | A directed protein interaction network for investigating intracellular signal transduction. | Science signaling | 258 | 21900206 |
| 2003 | Polo-like kinase 1 regulates Nlp, a centrosome protein involved in microtubule nucleation. | Developmental cell | 216 | 12852856 |
| 2014 | Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes. | Journal of proteomics | 215 | 25281560 |
| 2016 | An organelle-specific protein landscape identifies novel diseases and molecular mechanisms. | Nature communications | 211 | 27173435 |
| 2018 | An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations. | Nature communications | 201 | 29568061 |
| 2002 | Centrosomal proteins CG-NAP and kendrin provide microtubule nucleation sites by anchoring gamma-tubulin ring complex. | Molecular biology of the cell | 190 | 12221128 |
| 2000 | The centrosomal protein C-Nap1 is required for cell cycle-regulated centrosome cohesion. | The Journal of cell biology | 187 | 11076968 |
| 2020 | PROTAC-mediated degradation reveals a non-catalytic function of AURORA-A kinase. | Nature chemical biology | 129 | 32989298 |
| 2017 | The human cytoplasmic dynein interactome reveals novel activators of motility. | eLife | 118 | 28718761 |
| 2007 | Breast cancer molecular signatures as determined by SAGE: correlation with lymph node status. | Molecular cancer research : MCR | 90 | 17855657 |
| 2013 | Interaction of foot-and-mouth disease virus nonstructural protein 3A with host protein DCTN3 is important for viral virulence in cattle. | Journal of virology | 46 | 24352458 |
| 2013 | Dynactin Deficiency in the CNS of Humans with Sporadic ALS and Mice with Genetically Determined Motor Neuron Degeneration. | Neurochemical research | 33 | 24078265 |
| 2021 | Cellular and Viral Determinants of HSV-1 Entry and Intracellular Transport towards Nucleus of Infected Cells. | Journal of virology | 24 | 33472938 |
| 2019 | Effect of vitrification temperature and cryoprotectant concentrations on the mRNA transcriptome of bovine mature oocytes after vitrifying at immature stage. | Theriogenology | 21 | 31761539 |
| 2022 | Neuropathy-associated Fars2 deficiency affects neuronal development and potentiates neuronal apoptosis by impairing mitochondrial function. | Cell & bioscience | 18 | 35794642 |
| 2014 | Recurring DNA copy number gain at chromosome 9p13 plays a role in the activation of multiple candidate oncogenes in progressing oral premalignant lesions. | Cancer medicine | 15 | 25060540 |
| 2015 | Aberrant expression of maternal Plk1 and Dctn3 results in the developmental failure of human in-vivo- and in-vitro-matured oocytes. | Scientific reports | 13 | 25645239 |
| 2007 | Detection of novel amplicons in prostate cancer by comprehensive genomic profiling of prostate cancer cell lines using oligonucleotide-based arrayCGH. | PloS one | 12 | 17712417 |
| 2024 | Exposure to bisphenol A affects transcriptome-wide N6-methyladenine methylation in ovarian granulosa cells. | Ecotoxicology and environmental safety | 7 | 38354435 |
| 2021 | The expression, localisation and interactome of pigeon CRY2. | Scientific reports | 7 | 34645873 |
| 2024 | Whole-genome resequencing revealed the population structure and selection signal of 4 indigenous Chinese laying ducks. | Poultry science | 6 | 38781766 |
| 2022 | Network Meta-Analysis of Chicken Microarray Data following Avian Influenza Challenge-A Comparison of Highly and Lowly Pathogenic Strains. | Genes | 6 | 35327988 |
| 2024 | A proteome-wide association study identifies putative causal proteins for breast cancer risk. | British journal of cancer | 5 | 39468330 |
| 2019 | Predication of hub target genes of differentially expressed microRNAs contributing to Helicobacter pylori infection in gastric non-cancerous tissue. | Gastroenterology and hepatology from bed to bench | 4 | 32099601 |
| 2003 | Improving the comparative map of porcine chromosome 10 with respect to human chromosomes 1, 9 and 10. | Cytogenetic and genome research | 4 | 14970690 |
| 2023 | Sophora tonkinensis and active compounds inhibit mitochondrial impairments, inflammation, and LDLR deficiency in myocardial ischemia mice through regulating the vesicle-mediated transport pathway. | Fitoterapia | 3 | 38007052 |