{"gene":"DCTN3","run_date":"2026-06-13T19:06:35","timeline":{"discoveries":[],"current_model":"Parse failed"},"narrative":{"mechanistic_narrative":"No mechanistic discoveries found in literature.","teleology":[],"mechanism_profile":null},"prefetch_data":{"uniprot":{"accession":"O75935","full_name":"Dynactin subunit 3","aliases":["Dynactin complex subunit 22 kDa subunit","p22"],"length_aa":186,"mass_kda":21.1,"function":"Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). Together with dynein may be involved in spindle assembly and cytokinesis (PubMed:9722614)","subcellular_location":"Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Chromosome, centromere, kinetochore; Cytoplasm, cytoskeleton, spindle; Cleavage furrow; Midbody","url":"https://www.uniprot.org/uniprotkb/O75935/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":true,"resolved_as":"","url":"https://depmap.org/portal/gene/DCTN3","classification":"Common Essential","n_dependent_lines":1063,"n_total_lines":1208,"dependency_fraction":0.8799668874172185},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[{"gene":"CAPZB","stoichiometry":10.0},{"gene":"DCTN2","stoichiometry":10.0},{"gene":"DYNC1I2","stoichiometry":4.0},{"gene":"DYNC1LI1","stoichiometry":4.0},{"gene":"ACTG1","stoichiometry":0.2},{"gene":"CLASP1","stoichiometry":0.2},{"gene":"CLIP1","stoichiometry":0.2},{"gene":"DYNLL1","stoichiometry":0.2}],"url":"https://opencell.sf.czbiohub.org/search/DCTN3","total_profiled":1310},"omim":[{"mim_id":"607387","title":"DYNACTIN 3; DCTN3","url":"https://www.omim.org/entry/607387"}],"hpa":{"profiled":true,"resolved_as":"","reliability":"Supported","locations":[{"location":"Cytosol","reliability":"Supported"},{"location":"Nucleoli","reliability":"Additional"},{"location":"Perinuclear theca","reliability":"Additional"},{"location":"Calyx","reliability":"Additional"},{"location":"Mid piece","reliability":"Additional"},{"location":"Principal piece","reliability":"Additional"}],"tissue_specificity":"Low tissue specificity","tissue_distribution":"Detected in all","driving_tissues":[],"url":"https://www.proteinatlas.org/search/DCTN3"},"hgnc":{"alias_symbol":["DCTN-22"],"prev_symbol":[]},"alphafold":{"accession":"O75935","domains":[{"cath_id":"1.20.5","chopping":"1-29","consensus_level":"medium","plddt":79.9607,"start":1,"end":29}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/O75935","model_url":"https://alphafold.ebi.ac.uk/files/AF-O75935-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-O75935-F1-predicted_aligned_error_v6.png","plddt_mean":88.38},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=DCTN3","jax_strain_url":"https://www.jax.org/strain/search?query=DCTN3"},"sequence":{"accession":"O75935","fasta_url":"https://rest.uniprot.org/uniprotkb/O75935.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/O75935/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/O75935"}},"corpus_meta":[{"pmid":"17855657","id":"PMC_17855657","title":"Breast cancer molecular signatures as determined by SAGE: correlation with lymph node status.","date":"2007","source":"Molecular cancer research : MCR","url":"https://pubmed.ncbi.nlm.nih.gov/17855657","citation_count":90,"is_preprint":false},{"pmid":"24352458","id":"PMC_24352458","title":"Interaction of foot-and-mouth disease virus nonstructural protein 3A with host protein DCTN3 is important for viral virulence in cattle.","date":"2013","source":"Journal of virology","url":"https://pubmed.ncbi.nlm.nih.gov/24352458","citation_count":46,"is_preprint":false},{"pmid":"24078265","id":"PMC_24078265","title":"Dynactin Deficiency in the CNS of Humans with Sporadic ALS and Mice with Genetically Determined Motor Neuron Degeneration.","date":"2013","source":"Neurochemical research","url":"https://pubmed.ncbi.nlm.nih.gov/24078265","citation_count":33,"is_preprint":false},{"pmid":"33472938","id":"PMC_33472938","title":"Cellular and Viral Determinants of HSV-1 Entry and Intracellular Transport towards Nucleus of Infected Cells.","date":"2021","source":"Journal of virology","url":"https://pubmed.ncbi.nlm.nih.gov/33472938","citation_count":26,"is_preprint":false},{"pmid":"31761539","id":"PMC_31761539","title":"Effect of vitrification temperature and cryoprotectant concentrations on the mRNA transcriptome of bovine mature oocytes after vitrifying at immature stage.","date":"2019","source":"Theriogenology","url":"https://pubmed.ncbi.nlm.nih.gov/31761539","citation_count":21,"is_preprint":false},{"pmid":"35794642","id":"PMC_35794642","title":"Neuropathy-associated Fars2 deficiency affects neuronal development and potentiates neuronal apoptosis by impairing mitochondrial function.","date":"2022","source":"Cell & bioscience","url":"https://pubmed.ncbi.nlm.nih.gov/35794642","citation_count":19,"is_preprint":false},{"pmid":"25060540","id":"PMC_25060540","title":"Recurring DNA copy number gain at chromosome 9p13 plays a role in the activation of multiple candidate oncogenes in progressing oral premalignant lesions.","date":"2014","source":"Cancer medicine","url":"https://pubmed.ncbi.nlm.nih.gov/25060540","citation_count":15,"is_preprint":false},{"pmid":"25645239","id":"PMC_25645239","title":"Aberrant expression of maternal Plk1 and Dctn3 results in the developmental failure of human in-vivo- and in-vitro-matured oocytes.","date":"2015","source":"Scientific reports","url":"https://pubmed.ncbi.nlm.nih.gov/25645239","citation_count":13,"is_preprint":false},{"pmid":"17712417","id":"PMC_17712417","title":"Detection of novel amplicons in prostate cancer by comprehensive genomic profiling of prostate cancer cell lines using oligonucleotide-based arrayCGH.","date":"2007","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/17712417","citation_count":12,"is_preprint":false},{"pmid":"34645873","id":"PMC_34645873","title":"The expression, localisation and interactome of pigeon CRY2.","date":"2021","source":"Scientific reports","url":"https://pubmed.ncbi.nlm.nih.gov/34645873","citation_count":9,"is_preprint":false},{"pmid":"38781766","id":"PMC_38781766","title":"Whole-genome resequencing revealed the population structure and selection signal of 4 indigenous Chinese laying ducks.","date":"2024","source":"Poultry science","url":"https://pubmed.ncbi.nlm.nih.gov/38781766","citation_count":7,"is_preprint":false},{"pmid":"38354435","id":"PMC_38354435","title":"Exposure to bisphenol A affects transcriptome-wide N6-methyladenine methylation in ovarian granulosa cells.","date":"2024","source":"Ecotoxicology and environmental safety","url":"https://pubmed.ncbi.nlm.nih.gov/38354435","citation_count":7,"is_preprint":false},{"pmid":"39468330","id":"PMC_39468330","title":"A proteome-wide association study identifies putative causal proteins for breast cancer risk.","date":"2024","source":"British journal of cancer","url":"https://pubmed.ncbi.nlm.nih.gov/39468330","citation_count":6,"is_preprint":false},{"pmid":"35327988","id":"PMC_35327988","title":"Network Meta-Analysis of Chicken Microarray Data following Avian Influenza Challenge-A Comparison of Highly and Lowly Pathogenic Strains.","date":"2022","source":"Genes","url":"https://pubmed.ncbi.nlm.nih.gov/35327988","citation_count":6,"is_preprint":false},{"pmid":"38007052","id":"PMC_38007052","title":"Sophora tonkinensis and active compounds inhibit mitochondrial impairments, inflammation, and LDLR deficiency in myocardial ischemia mice through regulating the vesicle-mediated transport pathway.","date":"2023","source":"Fitoterapia","url":"https://pubmed.ncbi.nlm.nih.gov/38007052","citation_count":4,"is_preprint":false},{"pmid":"14970690","id":"PMC_14970690","title":"Improving the comparative map of porcine chromosome 10 with respect to human chromosomes 1, 9 and 10.","date":"2003","source":"Cytogenetic and genome research","url":"https://pubmed.ncbi.nlm.nih.gov/14970690","citation_count":4,"is_preprint":false},{"pmid":"32099601","id":"PMC_32099601","title":"Predication of hub target genes of differentially expressed microRNAs contributing to Helicobacter pylori infection in gastric non-cancerous tissue.","date":"2019","source":"Gastroenterology and hepatology from bed to bench","url":"https://pubmed.ncbi.nlm.nih.gov/32099601","citation_count":4,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":12960,"output_tokens":8,"usd":0.0195,"stage1_stop_reason":"refusal"},"stage2":{"model":"","input_tokens":0,"output_tokens":0,"usd":0.0},"total_usd":0.0195,"stage1_batch_id":"msgbatch_0147LFpL5iCPFZF66xp23BqC","stage2_batch_id":"msgbatch_01Crtwc6eCsZh1CrnfJhY7J7","note":"batch pricing = 50% of standard"},"stage1_raw":"","stage2_raw":"","audit_flag":{"gene":"DCTN3","tier":"GROUNDING","verdict":"Evidence-grounding concern","subtype":"recall_miss","uniprot_band":"medium","rules_fired":"R5","issue":"R5: no narrative despite experimental UniProt FUNCTION (227 chars) and on-target evidence in corpus (2 on-target corpus titles)"},"evaluation":null}