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Showing EIF4G2 — DAP5 is a alias.

EIF4G2

Eukaryotic translation initiation factor 4 gamma 2 · UniProt P78344

Length
907 aa
Mass
102.4 kDa
Annotated
2026-06-09
92 papers in source corpus 41 papers cited in narrative 41 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

EIF4G2 (DAP5/NAT1/p97) is a non-canonical eIF4G homolog that lacks the N-terminal eIF4E-binding domain and operates as a selective translation initiation scaffold for a discrete regulon of mRNAs, while being dispensable for bulk cap-dependent translation (PMID:9032289, PMID:25779044, PMID:27664238). It assembles with core initiation machinery—binding eIF4A through its MIF4G domain (with ~10-fold lower affinity than eIF4G1, which attenuates eIF4A helicase stimulation), eIF2beta through its C-terminal HEAT-repeat region, and eIF3d—to drive translation of mRNAs bearing long, structured 5'UTRs and upstream open reading frames (PMID:16932749, PMID:23478064, PMID:25779044, PMID:36473845). Mechanistically, EIF4G2 acts both through IRES-mediated cap-independent initiation, binding directly to structured 5'UTR elements (e.g., p53, FGF-9) via tertiary RNA folding rather than a defined sequence motif (PMID:23318444, PMID:38866431), and through eIF3d-dependent cap-dependent but eIF4E-independent recruitment, as well as by promoting leaky scanning and reinitiation downstream of translated uORFs—replacing eIF4G1 in scanning complexes when ribosomes encounter uORFs (PMID:35018467, PMID:36473845, PMID:39971159). Its target regulon encodes cell-cycle and survival regulators (Bcl-2, CDK1, MCL-1), tumor suppressors (PTEN, CREBBP/EP300), signaling kinases and phosphatases, and differentiation and chromatin factors (HMGN3, KMT2D), coupling EIF4G2 to specific cellular programs (PMID:18450493, PMID:27664238, PMID:36473845, PMID:35961752, PMID:42066769, PMID:42202060). Through this selective output, EIF4G2 is essential for embryonic development and stem-cell differentiation, controls oxidative respiration and mitochondrial gene programs, governs T-cell lineage commitment and regulatory T-cell stability, and functions as a translational checkpoint restraining tumor progression in breast, hepatocellular, pancreatic, and intestinal contexts (PMID:11032820, PMID:27664238, PMID:37314929, PMID:41457459, PMID:41940334, PMID:42066769, PMID:42202060). During apoptosis, caspase cleavage at position 790 removes an inhibitory C-terminal subdomain to generate a p86 fragment with enhanced IRES-stimulatory activity, sustaining its own and pro-apoptotic protein synthesis when cap-dependent translation is suppressed (PMID:10611228, PMID:11943866, PMID:18722383); viral proteases (coxsackievirus 2A, SARS-CoV-2 NSP5) likewise cleave EIF4G2 to redirect translation and, in the latter case, drive p53-dependent senescence (PMID:26586572, PMID:41924271). During neuronal activity, depolarization triggers phosphorylation and dendritic recruitment of EIF4G2 to uORF-containing mRNAs, coupling stimulation to local protein synthesis (PMID:38589584).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1997 Medium

    Established EIF4G2 as a distinct eIF4G-family protein, defining the structural basis for non-canonical function: it shares eIF4G homology but lacks the eIF4E-binding domain, and a dominant-negative fragment implicated it in cell-death regulation.

    Evidence Functional cDNA rescue screen and sequence analysis in HeLa cells, transfection-based death assays

    PMID:9032289

    Open questions at the time
    • No direct demonstration of translation activity
    • Mechanism of death protection not resolved
    • No interaction partners mapped
  2. 2000 High

    Defined the apoptotic processing of EIF4G2 and a self-sustaining circuit: caspase cleavage at position 790 yields p86 that complexes with eIF4A and eIF3, and an IRES in the EIF4G2 5'UTR maintains its own synthesis when cap-dependent translation fails.

    Evidence Caspase cleavage mapping, Co-IP, bicistronic reporters, and cell-free translation with recombinant protein

    PMID:10611228

    Open questions at the time
    • Identity of physiological IRES target mRNAs not defined
    • Quantitative contribution of p86 vs p97 unclear
    • Direct RNA binding not yet shown
  3. 2000 High

    Demonstrated EIF4G2 is essential in vivo and selectively required for differentiation programs rather than bulk translation, distinguishing it functionally from canonical eIF4G.

    Evidence Knockout mice (gastrulation lethality) and retinoic-acid differentiation assays in ES cells with expression profiling

    PMID:11032820

    Open questions at the time
    • Specific mRNA targets driving differentiation phenotype not identified
    • Molecular basis of selectivity unknown
  4. 2002 Medium

    Showed the caspase-generated p86 fragment, but not the analogous eIF4GI fragment, supports IRES-driven translation of apoptotic regulators, and identified an autoinhibitory C-terminal tail relieved by cleavage.

    Evidence IRES reporter assays and truncation-construct expression in cell culture under multiple stresses

    PMID:11943866 PMID:12458215

    Open questions at the time
    • Reporter-based; endogenous target regulation not quantified
    • Structural basis of autoinhibition not resolved at this stage
    • Single lab
  5. 2006 High

    Mapped the functional architecture and ribosomal engagement: the N-terminal MIF4G domain mediates ribosome localization, the C-terminus binds eIF2beta, and EIF4G2 supports proliferation through cell-cycle regulators.

    Evidence RNAi knockdown, ribosome fractionation, domain mapping, Co-IP of eIF2beta, and CDK2 kinase assays

    PMID:16932749

    Open questions at the time
    • Reported global translation effect later refined to selective targets
    • Direct mRNA binding not demonstrated
    • Recruitment signal unknown
  6. 2008 High

    Identified Bcl-2 and CDK1 as physiological targets explaining why EIF4G2 loss triggers M-phase apoptosis, linking its selective translation output to cell survival and mitotic progression.

    Evidence RNAi, polysome profiling, IRES reporters, CDK1 substrate Westerns, and rescue by ectopic Bcl-2/CDK1

    PMID:18450493

    Open questions at the time
    • Direct binding to Bcl-2/CDK1 5'UTRs not shown here
    • Full target regulon undefined
  7. 2008 High

    Provided structural rationale for caspase regulation: the C-terminal HEAT-repeat domain resembles eIF4GI/eIF5/eIF2Bepsilon, with a disordered, poorly stabilized loop at the cleavage site explaining protease accessibility and the loss of eIF2beta-interacting acidic residues upon cleavage.

    Evidence X-ray crystallography of the C-terminal domain (aa 730–897)

    PMID:18722383

    Open questions at the time
    • Structure of the full-length protein and MIF4G not yet solved
    • RNA-binding surface not localized
  8. 2013 High

    Quantified the EIF4G2–eIF4A interaction and gave the first direct demonstration of EIF4G2–mRNA binding, establishing sequence-specific IRES recognition (p53) as part of its mechanism.

    Evidence MIF4G crystal structure with ITC and in vitro unwinding assays; EMSA and RNA immunoprecipitation for p53 IRES binding

    PMID:23318444 PMID:23478064

    Open questions at the time
    • Generality of direct binding across targets not established at this point
    • Structural basis of RNA recognition unresolved
  9. 2015 High

    Consolidated the core initiation model—EIF4G2 partners with eIF2beta and eIF4AI to drive cellular IRES translation while being dispensable for cap-dependent translation—and revealed tissue-specific and signaling-gated roles plus species-conserved circadian and developmental functions.

    Evidence Co-IP and IRES/in vitro translation assays (human); Drosophila tissue-specific RNAi in spermatogenesis; earlier circadian RNAi in pacemaker neurons

    PMID:22904033 PMID:25779044 PMID:25849588

    Open questions at the time
    • Genome-wide target set not yet defined
    • Mechanism distinguishing IRES vs scanning roles unresolved
  10. 2016 High

    Defined the genome-wide EIF4G2 translatome in human stem cells, linking it to mitochondrial/oxidative-respiration proteins and differentiation factors (HMGN3) and explaining the differentiation and apoptosis phenotypes of EIF4G2 loss.

    Evidence Polysome-seq, knockdown in hESCs, embryoid body and mitochondrial respiration assays, IRES reporters

    PMID:27664238

    Open questions at the time
    • Distinction between direct and indirect targets incomplete
    • Initiation mechanism for each target class not parsed
  11. 2019 Medium

    Expanded the mechanism to additional physiological and viral contexts: signaling-gated eIF2beta binding, hypoxia-relevant PHD2 translation, neuronal axon-outgrowth control, viral-protease cleavage redirecting translation, and a PCBP2 regulatory feedback loop.

    Evidence Co-IP and knockdown with PHD2/HIF-1alpha readouts; DSCR1.4 5'UTR binding and axon assays; CVB3 2A cleavage mapping and type-I IRES dependence; PCBP2 in vitro translation assays

    PMID:26586572 PMID:29530922 PMID:30718468 PMID:31010886 PMID:31455634

    Open questions at the time
    • Most single-lab; mechanistic generality across contexts uncertain
    • Phospho-sites and signaling inputs incompletely mapped
  12. 2021 High

    Established quantitative 5'UTR recognition correlating with translation efficiency and revealed a non-canonical cap-dependent, eIF4E-independent DAP5/eIF3d mechanism governing Treg differentiation.

    Evidence Fluorescence anisotropy 5'UTR binding (HIF-1alpha/FGF-9/p53) and in vitro translation; ribosome profiling and knockdown in primary human CD4+ T cells

    PMID:32571876 PMID:34848685

    Open questions at the time
    • How EIF4G2/eIF3d selects cap-structured mRNAs not fully resolved
    • Relationship between IRES and cap-dependent modes unclear
  13. 2022 High

    Resolved a distinct scanning/reinitiation mechanism: EIF4G2 promotes leaky scanning and reinitiation downstream of translated uORFs on mRNAs with long structured leaders, replacing eIF4G1 in scanning complexes, with cap/eIF4F-dependent recruitment supporting main-CDS but not uORF translation.

    Evidence Ribosome profiling, uORF-containing luciferase reporters with mutational analysis, Co-IP, CLIP in hESCs

    PMID:35018467 PMID:35961752 PMID:36473845

    Open questions at the time
    • Switch between IRES and scanning/reinitiation modes not mechanistically defined
    • Direct binding frequent but not absolutely required—determinants unclear
  14. 2023 High

    Tied EIF4G2-selective translation to disease: a DAP5/eIF3d complex drives EMT and metastasis programs, EIF4G2 supports HCC growth via PLEKHA1 IRES translation and FXTAS RAN translation, and cancer-associated MIF4G mutations dissociate its IRES versus uORF functions.

    Evidence Translatome profiling and Co-IP in breast cancer with in vivo metastasis assays; polysome/RIP in HCC; Drosophila FXTAS genetic epistasis; structure-function mutagenesis

    PMID:37314929 PMID:37352983 PMID:38129098 PMID:39213495

    Open questions at the time
    • Functional separation of EIF4G2's mechanistic modes incompletely mapped to structure
    • Some disease links rest on single models
  15. 2024 Medium

    Extended EIF4G2's mechanism beyond initiation and into activity-dependent neuronal control: it stimulates eRF3 GTPase activity in translation termination via its MIF4G domain, and depolarization-driven phosphorylation recruits it to dendritic uORF-containing mRNAs for local synthesis.

    Evidence Reconstituted termination system with eRF3 GTPase assay (preprint); dendritic APEX proximity labeling, CLIP, ribosome profiling in cortical neurons

    PMID:38589584 PMID:bio_10.1101_2024.09.10.612082

    Open questions at the time
    • Termination role from a single preprint, not peer-reviewed
    • Phosphorylation sites and responsible kinase not fully defined
  16. 2025 High

    Defined EIF4G2 as a tumor-restraining translational checkpoint and immune-lineage regulator in vivo, and characterized viral hijacking driving senescence: it selectively translates structured-5'UTR tumor suppressors (Pten, Crebbp/Ep300, KAT3 coactivators), sustains Treg and CD8 lineage programs, and SARS-CoV-2 NSP5 cleavage redirects DAP5 to a nuclear p53/CDKN1A-activating fragment countered by TRIM7.

    Evidence In vivo CRISPR screens and conditional knockouts (PDAC, intestine, Treg, T-cell) with ribosome profiling and chromatin profiling; cleavage mapping, ChIP-seq, Co-IP and ubiquitination assays for NSP5/TRIM7

    PMID:38138978 PMID:41457459 PMID:41924271 PMID:41940334 PMID:42066769 PMID:42202060

    Open questions at the time
    • Mechanistic basis for tumor-context-dependent target selection incomplete
    • Some translatomic targets validated in single models

Open questions

Synthesis pass · forward-looking unresolved questions
  • How EIF4G2 chooses among its distinct mechanistic modes—IRES recognition, eIF3d-dependent cap-dependent recruitment, leaky-scanning/reinitiation, and termination—on a given target, and what signals or RNA features dictate that choice, remains unresolved.
  • No unified model linking 5'UTR structural features to a specific initiation mode
  • Phospho-regulation and cofactor switching incompletely mapped
  • No full-length structure of EIF4G2 on a target mRNA

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 5 GO:0045182 translation regulator activity 5 GO:0060090 molecular adaptor activity 4 GO:0140110 transcription regulator activity 2
Localization
GO:0005634 nucleus 3 GO:0005829 cytosol 2 GO:0005840 ribosome 1
Pathway
R-HSA-1266738 Developmental Biology 4 R-HSA-392499 Metabolism of proteins 4 R-HSA-1643685 Disease 3 R-HSA-168256 Immune System 3 R-HSA-5357801 Programmed Cell Death 3 R-HSA-8953854 Metabolism of RNA 3
Partners
EIF2S2EIF3DEIF4A1ETF1PCBP2
Complex memberships
DAP5/eIF3d complex

Evidence

Reading pass · 41 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 DAP5 (EIF4G2) was identified as a novel 97-kDa protein homologous to eIF4G that lacks the N-terminal eIF4E-binding domain; a dominant-negative C-terminal fragment (28 kDa miniprotein) protected HeLa cells from IFN-gamma-induced programmed cell death when expressed at low levels. Functional cDNA rescue screen in HeLa cells, full-length cDNA cloning, sequence analysis, transfection-based cell death assays Molecular and cellular biology Medium 9032289
2000 During apoptosis (Fas or p53 activation), DAP5 is cleaved by caspases at position 790, yielding an 86-kDa C-terminal isoform (DAP5/p86) that forms complexes with eIF4A and eIF3. An IRES element in the DAP5 5'UTR maintains DAP5 translation during apoptosis when cap-dependent translation is suppressed, and recombinant DAP5/p97 or DAP5/p86 enhanced translation through the DAP5 IRES in cell-free systems, establishing a positive feedback loop. Western blot caspase cleavage mapping, Co-IP (eIF4A, eIF3), bicistronic reporter assays, cell-free translation assays with recombinant protein, dominant-negative neutralization in reticulocyte lysates Molecular and cellular biology High 10611228
2000 NAT1/DAP5 knockout mice die during gastrulation; NAT1-/- embryonic stem cells show impaired differentiation in response to retinoic acid and selectively reduced expression of retinoic acid-responsive genes (e.g., p21WAF1), without defects in global translation or proliferation in undifferentiated cells. Gene knockout in mice, ES cell differentiation assays, teratoma formation, gene expression profiling The EMBO journal High 11032820
2002 The caspase-cleaved DAP5/p86 fragment, but not the eIF4GI M-FAG/p76 fragment, supports cap-independent IRES-mediated translation of apoptosis-related proteins including c-Myc, Apaf-1, DAP5, and XIAP. The C-terminal tail of DAP5/p97 exerts an inhibitory effect on cap-independent translation that is relieved by caspase cleavage. Transfection-based IRES reporter assays in cell culture, ectopic expression of DAP5 truncation constructs Proceedings of the National Academy of Sciences of the United States of America Medium 11943866
2002 Caspase-cleaved apoptotic fragments of DAP5/p97 (p86) and eIF4GI correlate with activation of pro-death IRES elements during etoposide-induced apoptosis, while pro-survival IRES elements are active under milder stress, demonstrating that these fragments differentially regulate IRES translation based on stress severity. IRES reporter assays in cell culture under various stresses, Western blot of caspase cleavage products The Journal of biological chemistry Medium 12458215
2006 p97/DAP5 is recruited to ribosomes following growth factor stimulation, binds eIF2beta through its C-terminal domain, and localizes to ribosomes through its N-terminal MIF4G domain. Knockdown of p97 by RNAi decreases global translation rate, inhibits cell proliferation, increases p27/Kip1 protein, and decreases CDK2 kinase activity. RNAi knockdown, ribosome fractionation, domain mapping by overexpression of truncation constructs, Co-IP (eIF2beta), luciferase reporter assays, CDK2 kinase assay The EMBO journal High 16932749
2007 DAP5/p97 expression is selectively enhanced during ER stress by IRES-mediated translation of its own mRNA, providing a positive feedback loop. The full-length DAP5/p97 activates the HIAP2 IRES in a caspase-independent manner during ER stress, while HIAP2 IRES activation requires subsequent caspase-dependent proteolytic processing of DAP5/p97 to p86. Polysome profiling, bicistronic IRES reporter assays, caspase inhibitor experiments (z-VAD-fmk), Western blotting Nucleic acids research Medium 18003655
2008 DAP5 knockdown induces M-phase-specific caspase-dependent apoptosis in non-stressed cells. Bcl-2 and CDK1 mRNAs are DAP5 translation targets (both containing functional IRES elements in their 5'UTRs); DAP5 knockdown shifts Bcl-2 mRNA to light polysomes and reduces CDK1-dependent phosphorylation of M-phase substrates. Ectopic expression of Bcl-2 or CDK1 partially rescues caspase activation caused by DAP5 knockdown. RNAi knockdown, polysome profiling, IRES reporter assays, flow cytometry (M-phase apoptosis), Western blot of CDK1 substrates, rescue by ectopic expression Molecular cell High 18450493
2008 Crystal structure of the C-terminal region of DAP5/p97 (aa 730–897) reveals four HEAT repeats homologous to eIF4GI, eIF5, and eIF2Bepsilon. The loop connecting alpha3 and alpha4 (harboring the caspase cleavage site at position 792) lacks electron density and shows fewer stabilizing interactions, explaining caspase accessibility. The caspase cleavage removes a subdomain carrying acidic residues of the AA-box motif, likely altering protein–protein interactions (e.g., with eIF2beta). X-ray crystallography of C-terminal DAP5 domain (aa 730–897) Journal of molecular biology High 18722383
2008 DAP5/p97 is induced by ATRA during granulocytic differentiation of APL cells, undergoes nuclear translocation, and its knockdown inhibits ATRA-induced differentiation and ATO-induced apoptosis. DAP5/p97 expression is upregulated by inhibition of the PI3K/Akt/mTOR pathway. siRNA knockdown, Western blot, immunofluorescence (nuclear translocation), PI3K/Akt/mTOR pathway inhibitors Apoptosis Medium 18491231
2012 NAT1/DAP5 knockdown in Drosophila circadian pacemaker neurons lengthens circadian period and dramatically reduces PER and BELLE protein levels, indicating that NAT1 promotes translation of per mRNA and belle mRNA (a NAT1 target). TOR kinase inhibition increases circadian oscillator activity in a NAT1-dependent manner, suggesting NAT1 mediates cap-independent translation relevant to the circadian oscillator. Targeted RNAi screen, circadian behavioral assays, immunostaining of PER and BELLE in PDF neurons, TOR inhibitor experiments Genetics Medium 22904033
2013 Crystal structure of the DAP5 MIF4G domain reveals overall HEAT-repeat solenoid fold similar to eIF4G, but with distinct surface properties. ITC quantification shows DAP5 binds eIF4A with ~10-fold lower affinity than eIF4G, and this reduced affinity attenuates eIF4A RNA helicase/unwinding activity stimulation in vitro. X-ray crystallography, isothermal titration calorimetry (ITC), in vitro RNA unwinding assay Structure High 23478064
2013 DAP5 promotes IRES-driven translation of p53 mRNA, preferentially from the second IRES in the coding sequence that drives Δ40p53 isoform production. DAP5 knockdown shifts p53 mRNA to lighter polysomes. DAP5 directly binds p53 IRES elements in vitro (EMSA) and in vivo (RNA immunoprecipitation), representing the first demonstration of direct DAP5–mRNA binding. siRNA knockdown, bicistronic reporter assays, polysome profiling, EMSA (in vitro RNA binding), RNA immunoprecipitation (in vivo) Oncogene High 23318444
2015 DAP5 associates with eIF2beta and eIF4AI to stimulate IRES-dependent translation of cellular mRNAs. DAP5 is dispensable for cap-dependent translation. These interactions were demonstrated by Co-IP and functional translation assays. Co-immunoprecipitation, siRNA knockdown, IRES reporter assays, in vitro translation assays Nucleic acids research High 25779044
2015 In Drosophila spermatogenesis, eIF4G2 (testes-specific) is required in early germ cells for proper meiotic divisions and spermatid elongation; abrogation in spermatocytes causes meiotic arrest. Double knockdown of eIF4G and eIF4G2 shows redundancy during early spermatogenesis. eIF4G2 has distinct, spatio-temporally restricted roles compared to canonical eIF4G. UAS-Gal4 RNAi knockdown in defined cell populations, male fertility assays, histological analysis of spermatogenesis stages PloS one Medium 25849588
2015 Coxsackievirus B3 2A protease (not 3C) cleaves DAP5 at amino acid G434, generating 45-kDa N-terminal (DAP5-N) and 52-kDa C-terminal (DAP5-C) fragments. DAP5-N translocates to the nucleus at late infection timepoints while DAP5-C remains cytoplasmic. DAP5-N retains ability to drive IRES-dependent translation of pro-apoptotic p53 but not pro-survival Bcl-2, promotes CVB3 replication, while DAP5-C acts as dominant-negative for cap-dependent translation. Site-directed mutagenesis to map cleavage site, subcellular fractionation/immunofluorescence, IRES reporter assays, viral replication assays Cell death and differentiation High 26586572
2016 DAP5 knockdown from human ESCs results in persistence of pluripotent gene expression, delayed induction of differentiation genes, defective embryoid body formation, and enhanced mislocalized apoptosis. Polysome-seq identified mitochondrial proteins involved in oxidative respiration as DAP5 translation targets; DAP5 KD cells show aberrant mitochondrial morphology and decreased oxidative respiratory activity. HMGN3 was identified as a cap-independent DAP5 translation target whose knockdown causes defective differentiation. siRNA/shRNA knockdown in hESCs, RNA sequencing of polysome-associated mRNAs, embryoid body assay, mitochondrial morphology imaging, respiration assays, IRES reporter assays Genes & development High 27664238
2018 DAP5 binds eIF2beta via a PKC-Raf-ERK1/2 signal-dependent interaction that determines DAP5's influence on translation. DAP5 depletion causes a surge of HIF-1alpha by reducing translation of PHD2 (the oxygen-sensing prolyl hydroxylase that hydroxylates HIF-1alpha for degradation); this DAP5:eIF2beta-dependent PHD2 translation occurs during hypoxia-associated protein synthesis repression. Co-IP (DAP5:eIF2beta), siRNA knockdown of DAP5, Western blot of PHD2 and HIF-1alpha, pharmacological PKC/ERK signaling manipulation, hypoxia experiments Molecular and cellular biology Medium 29530922
2019 DAP5 is specifically required by type I IRES (CVB3) but not type II or type III IRES elements for cap-independent translation. DAP5 (but not full-length eIF4GI) facilitates initial-round translation of CVB3 input RNA, because DAP5 structurally resembles the C-terminal eIF4GI fragment produced by 2A protease cleavage. DAP5 and C-terminal eIF4GI bind the same region of CVB3 IRES but DAP5 has lower affinity. siRNA knockdown, IRES reporter assays for type I/II/III IRES, RNA binding assays, viral replication assays The Journal of biological chemistry Medium 31455634
2019 PCBP2 isoform f interacts with the 5'UTR of eIF4G2 mRNA and inhibits eIF4G2 translation in vitro and in cells. Reciprocally, eIF4G2 participates in cap-dependent translation of PCBP2 mRNA, forming a feedback loop between the translation factor and the RNA-binding protein. In vitro translation assays, PCBP2 isoform transfection, reporter assays, co-immunoprecipitation/RNA binding studies RNA Medium 31010886
2019 DAP5 enhances cap-independent translation of DSCR1.4 mRNA in hippocampal neurons, binds directly to the DSCR1.4 5'UTR, and promotes axonal outgrowth. BDNF stimulation increases DAP5 expression and DSCR1.4 cap-independent translation efficiency in both soma and axons. siRNA knockdown, bicistronic reporter assays, RNA binding (DSCR1.4 5'UTR), axon outgrowth measurement, BDNF stimulation experiments Cell death & disease Medium 30718468
2020 eIF4GI and DAP5 specifically bind to the 5'UTRs of cap-independently translated mRNAs (HIF-1alpha, FGF-9, p53) as demonstrated by fluorescence anisotropy binding studies. The binding affinity of eIF4GI and DAP5 to these 5'UTRs correlates with translation efficiency in vitro. The eIF4E-binding domain of eIF4GI increases binding affinity and selectivity among these mRNAs. Fluorescence anisotropy equilibrium binding, luciferase reporter-based in vitro translation assays, mutational analysis of 5'UTRs The Journal of biological chemistry Medium 32571876
2021 TGF-beta-induced Treg cell differentiation utilizes a DAP5/eIF3d non-canonical cap-dependent (eIF4E-independent) translation mechanism. Treg cell mRNAs utilize DAP5 and eIF3d directed by 5' noncoding regions, while mTORC1/eIF4E/eIF4G-dependent translation of other T cell mRNAs is impaired. Silencing DAP5 in naive human CD4+ T cells impairs their differentiation into Treg cells. Genome-wide transcription and translation profiling (ribosome profiling), siRNA knockdown of DAP5 in primary human T cells, Treg differentiation assay, mTORC1 inhibition experiments Nature communications High 34848685
2022 eIF4G2 promotes scanning downstream of eIF4G1-mediated 40S recruitment; specifically, eIF4G2 facilitates leaky scanning for a subset of mRNAs containing translated uORFs. eIF4G2 appears to replace eIF4G1 during scanning when eIF4G1 dissociates from the scanning complex, e.g., when leaky scanning complexes interfere with ribosomes translating uORFs. Ribosome profiling upon eIF4G2 knockdown, luciferase reporters with uORF-containing 5'UTRs, mutational analysis Nucleic acids research High 35018467
2022 DAP5-mediated translation occurs on mRNAs with long, structure-prone 5' leader sequences and persistent uORF translation. Cap/eIF4F- and eIF4A-dependent recruitment of DAP5 to mRNA facilitates main CDS but not uORF translation, suggesting a role for DAP5 in translation re-initiation. DAP5 target mRNAs preferentially encode signaling kinases and phosphatases. Ribosome profiling (DAP5 knockdown), luciferase reporters with mutational analysis, co-immunoprecipitation Nature communications High 36473845
2022 Ribosome profiling in DAP5 knockdown hESCs identified 68 mRNAs with decreased translation efficiency, including KMT2D. Nearly half of DAP5 target mRNAs contain actively translated uORFs upstream of the main coding sequence, consistent with DAP5 mediating leaky scanning through uORFs and/or reinitiation. CLIP experiments showed that direct DAP5–mRNA binding is a frequent but not absolute requirement for DAP5-dependent translation. Ribosome profiling, mass spectrometry (protein abundance), crosslinking immunoprecipitation (CLIP), siRNA knockdown in hESCs RNA High 35961752
2023 DAP5/eIF3d forms a complex that mediates selective cap-dependent, eIF4E-independent translation of mRNAs encoding EMT transcription factors, cell migration integrins, metalloproteinases, and survival/angiogenesis factors. DAP5 is required for EMT, cell migration, invasion, metastasis, angiogenesis, and anoikis resistance in breast cancer, but not for primary tumor growth. Genome-wide transcriptomics and translatomics (translatome profiling), DAP5 knockdown in human and murine breast cancer models, Co-IP (DAP5/eIF3d complex), in vivo metastasis assays Cell reports High 37314929
2023 Loss-of-function cancer-associated missense mutations in EIF4G2 MIF4G domain selectively impair either protein–protein interactions or IRES-dependent translation initiation without affecting uORF-dependent translation. One mutation (R178Q) causes near-complete loss of EIF4G2 function and reduced protein expression. Functional assays of cancer-derived missense mutations, IRES reporter assays, protein–protein interaction assays (Co-IP/pulldown), uORF reporter assays Life science alliance Medium 38129098
2023 DAP5 knockdown in a Drosophila FXTAS model robustly suppresses CGG repeat-associated toxicity and inhibits RAN translation. Knockdown of initiation factors that preferentially associate with DAP5 (eIF2beta, eIF3F, eIF3G) also selectively suppresses CGG repeat-induced neurodegeneration, placing DAP5 and its interactors in the pathway of RAN translation initiation. Drosophila RNAi knockdown, eye degeneration scoring, RAN translation reporter assays, mammalian cellular reporter assays Neurobiology of disease Medium 37352983
2023 eIF4G2 facilitates PLEKHA1 protein translation via an IRES-dependent mechanism in hepatocellular carcinoma cells. EIF4G2 deletion suppresses tumor growth and metastasis in vitro and in vivo. PLEKHA1 was identified as a key translational product of EIF4G2 by polysome analysis and nascent protein synthesis assays; RIP and dual-luciferase reporter assays confirmed IRES-dependent mechanism. CRISPR/siRNA knockdown, polysome profiling, nascent protein synthesis assay, RNA immunoprecipitation (RIP), dual-luciferase reporter assay, in vivo xenograft Journal of proteome research Medium 39213495
2023 eIF4G2 promotes translation of both POLGARF and POLG (from overlapping reading frames on the human POLG mRNA) by enhancing both leaky scanning and reinitiation downstream of a regulatory uORF. Ribosomes can acquire eIF4G2 during early reinitiation steps. Luciferase reporters with uORF-containing POLG 5'UTR, mutational analysis, siRNA knockdown, ribosome profiling International journal of molecular sciences Medium 38138978
2024 Neuronal depolarization causes rapid reprogramming of dendritic translation accompanied by phosphorylation and recruitment of eIF4G2 to dendrites. eIF4G2 binds upstream open reading frames (uORFs) in pre-localized dendritic mRNAs, and the translated uORFs are sufficient to confer depolarization-induced, eIF4G2-dependent translational control of downstream coding sequences involved in long-term potentiation, cell signaling, and energy metabolism. Dendritically targeted proximity labeling (APEX), CLIP, ribosome profiling, mass spectrometry, reporter assays in primary cortical neurons, KCl/DHPG depolarization Nature neuroscience High 38589584
2024 SHAPE-seq structural modeling of the FGF-9 5'UTR combined with DAP5 footprinting, toeprinting, and UV cross-linking identifies DAP5 binding to a tertiary structural face of the FGF-9 5'UTR near the start codon. DAP5 binding appears to involve tertiary RNA folding rather than a conserved sequence or secondary structure motif. SHAPE-seq, DAP5 RNA footprinting, toeprinting, UV cross-linking RNA Medium 38866431
2025 eIF3d and eIF4G2 form a complex that recruits a subset of cap-structured mRNAs to ribosomes in an eIF4E-independent but cap-dependent manner. eIF3d binding to fully methylated 5' cap structure is quantitatively demonstrated; affinity of eIF3d and eIF3d/eIF4G2 complex binding to mRNA 5'UTRs correlates with translation efficiency, providing an alternative to canonical eIF4E-mediated initiation under cellular stress. Fluorescence anisotropy equilibrium binding (eIF3d and eIF3d/eIF4G2 to mRNA), in vitro translation assays, cap methylation manipulation The Journal of biological chemistry Medium 39971159
2025 SARS-CoV-2 NSP5 cleaves DAP5 at a specific site, producing an N-terminal fragment (DAP51-451) that translocates to the nucleus, interacts with p53, binds the CDKN1A locus to increase its expression (causing cell cycle arrest), and activates NF-κB/SASP, thereby driving virus-induced cellular senescence. Apoptosis-activated caspase-3 also cleaves DAP5 in a different positive feedback loop. Host TRIM7 E3 ligase targets DAP51-451 for glutamine C-degron-mediated ubiquitination and degradation, restricting viral replication. Western blot mapping of cleavage sites, FRET analysis, ChIP-seq, dual-luciferase reporter assays, Co-IP (DAP51-451 with p53), ubiquitination assays Frontiers in immunology Medium 41924271
2025 Homozygous Dap5 deletion in Tregs causes spontaneous scurfy-like autoimmunity with intact thymic and peripheral development; Dap5 haploinsufficiency in Tregs preserves immune homeostasis while suppressing tumor growth. DAP5 mediates alternate translation of transcripts encoding CD25 and MCL-1 in Tregs, sustaining Treg lineage stability and survival in the tumor microenvironment. Conditional/homozygous Dap5 knockout mice (Treg-specific), tumor growth assays, CD8+ T cell infiltration analysis, ribosome profiling/translatomics in Tregs Advanced science Medium 41457459
2025 eIF4G2 directs CD8+ T cell lineage commitment by selectively enabling IL-7 receptor (IL-7R) signaling; T cell-specific eIF4G2 deletion abolishes CD8+ single-positive thymocyte lineage commitment while sparing CD4+ lineage. Mechanistically, eIF4G2 deficiency fails to sustain translation of the IL-7R gamma-chain (gamma-c) via a UTR-dependent mechanism, and also impairs IL-7Ralpha mRNA levels. T cell-specific conditional knockout mice, flow cytometry of thymic populations, IL-7 signaling assays, UTR-dependent reporter assays iScience Medium 41940334
2025 Genetic interaction screen in Drosophila identifies a functional interaction between Gemin3/Ddx20 and NAT1/eIF4G2; loss of NAT1 downregulates Gemin3 mRNA levels. Both factors share convergent transcriptome alterations including requirements in actin cytoskeleton organization, neurodevelopment (brain growth), and organism development, despite no direct physical association detected. Unbiased genetic screen in Drosophila, RNAi knockdown, transcriptome analysis, brain morphology imaging, muscle contraction assays Developmental biology Low 39924071
2025 eIF4G2 loss in adult mouse intestine (inducible knockout) collapses Lgr5+ intestinal stem cell and secretory maturation programs, activates a fetal-like/regenerative state with YAP-TEAD activation. Ribosome profiling reveals selective translation-efficiency loss among chromatin regulators, especially KAT3 coactivators CREBBP and EP300, resulting in reduced histone acetylation. CUT&Tag and ATAC-seq show eIF4G2-KAT3 output drives locus-selective enhancer remodeling. Inducible Eif4g2 knockout mice, intestinal organoids, ribosome profiling, single-nucleus multiome, CUT&Tag, ATAC-seq, KAT3 chemical inhibition Cell stem cell High 42066769
2026 In vivo CRISPR/Cas9 screen identifies eIF4G2 as a translational checkpoint restraining pancreatic ductal adenocarcinoma (PDAC) progression. eIF4G2 loss accelerates tumor growth, promotes basal-like/poorly differentiated histology, and triggers widespread metastasis. Ribosome profiling reveals eIF4G2 selectively translates mRNAs with long, GC-rich, structured 5'UTRs including tumor suppressors Pten and Crebbp; eIF4G2 loss does not alter bulk protein synthesis. In vivo CRISPR/Cas9 screen, ribosome profiling, in vivo tumor models, patient-derived PDAC cell functional assays Cancer research High 42202060
2024 eIF4G2 also promotes translation termination in a reconstituted mammalian system; eIF4G2/DAP5 can stimulate the GTPase activity of eRF3 via its MIF4G domain, facilitating release factor dissociation after peptide release as part of a closed-loop mRNA structure. Reconstituted mammalian translation termination system, domain deletion analysis (MIF4G), eRF3 GTPase assay bioRxivpreprint Medium bio_10.1101_2024.09.10.612082

Source papers

Stage 0 corpus · 92 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1999 Delay-dependent impairment of a matching-to-place task with chronic and intrahippocampal infusion of the NMDA-antagonist D-AP5. Hippocampus 452 10226773
1992 The NMDA receptor antagonist D-2-amino-5-phosphonopentanoate (D-AP5) impairs spatial learning and LTP in vivo at intracerebral concentrations comparable to those that block LTP in vitro. The Journal of neuroscience : the official journal of the Society for Neuroscience 393 1345945
1984 L-glutamate has higher affinity than other amino acids for [3H]-D-AP5 binding sites in rat brain membranes. Nature 276 6141527
2000 A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation. Molecular and cellular biology 160 10611228
2002 The caspase-cleaved DAP5 protein supports internal ribosome entry site-mediated translation of death proteins. Proceedings of the National Academy of Sciences of the United States of America 133 11943866
2008 DAP5 promotes cap-independent translation of Bcl-2 and CDK1 to facilitate cell survival during mitosis. Molecular cell 125 18450493
2002 Distinct regulation of internal ribosome entry site-mediated translation following cellular stress is mediated by apoptotic fragments of eIF4G translation initiation factor family members eIF4GI and p97/DAP5/NAT1. The Journal of biological chemistry 124 12458215
1997 DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death. Molecular and cellular biology 121 9032289
2006 Coxsackievirus B3 proteases 2A and 3C induce apoptotic cell death through mitochondrial injury and cleavage of eIF4GI but not DAP5/p97/NAT1. Apoptosis : an international journal on programmed cell death 113 17195095
2000 Essential role of NAT1/p97/DAP5 in embryonic differentiation and the retinoic acid pathway. The EMBO journal 88 11032820
2015 DAP5 associates with eIF2β and eIF4AI to promote Internal Ribosome Entry Site driven translation. Nucleic acids research 79 25779044
2013 The translation initiation factor DAP5 promotes IRES-driven translation of p53 mRNA. Oncogene 72 23318444
2007 The eIF4G homolog DAP5/p97 supports the translation of select mRNAs during endoplasmic reticulum stress. Nucleic acids research 71 18003655
2006 p97/DAP5 is a ribosome-associated factor that facilitates protein synthesis and cell proliferation by modulating the synthesis of cell cycle proteins. The EMBO journal 71 16932749
2015 miR-139-5p controls translation in myeloid leukemia through EIF4G2. Oncogene 54 26165837
2023 An in vitro-transcribed circular RNA targets the mitochondrial inner membrane cardiolipin to ablate EIF4G2+/PTBP1+ pan-adenocarcinoma. Nature cancer 51 37845485
2013 N-methyl-d-aspartate receptors, learning and memory: chronic intraventricular infusion of the NMDA receptor antagonist d-AP5 interacts directly with the neural mechanisms of spatial learning. The European journal of neuroscience 50 23311352
2016 Cap-independent translation by DAP5 controls cell fate decisions in human embryonic stem cells. Genes & development 49 27664238
2017 Suppression of EIF4G2 by miR-379 potentiates the cisplatin chemosensitivity in nonsmall cell lung cancer cells. FEBS letters 48 28117895
2017 MicroRNA-379 inhibits the proliferation, migration and invasion of human osteosarcoma cells by targetting EIF4G2. Bioscience reports 45 28381518
2021 A DAP5/eIF3d alternate mRNA translation mechanism promotes differentiation and immune suppression by human regulatory T cells. Nature communications 44 34848685
2016 miR-139 is up-regulated in osteoarthritis and inhibits chondrocyte proliferation and migration possibly via suppressing EIF4G2 and IGF1R. Biochemical and biophysical research communications 43 27105918
2020 5'-UTR recruitment of the translation initiation factor eIF4GI or DAP5 drives cap-independent translation of a subset of human mRNAs. The Journal of biological chemistry 42 32571876
2024 Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding. Nature neuroscience 39 38589584
2020 LncRNA SDHAP1 confers paclitaxel resistance of ovarian cancer by regulating EIF4G2 expression via miR-4465. Journal of biochemistry 38 32211849
2015 Loss-of-function analysis reveals distinct requirements of the translation initiation factors eIF4E, eIF4E-3, eIF4G and eIF4G2 in Drosophila spermatogenesis. PloS one 38 25849588
2019 LINC01579 promotes cell proliferation by acting as a ceRNA of miR-139-5p to upregulate EIF4G2 expression in glioblastoma. Journal of cellular physiology 37 31187495
1989 Subchronic administration of MK-801 in the rat decreases cortical binding of [3H]D-AP5, suggesting down-regulation of the cortical N-methyl-D-aspartate receptors. Neuroscience 37 2568602
2023 Breast cancer cell mesenchymal transition and metastasis directed by DAP5/eIF3d-mediated selective mRNA translation. Cell reports 35 37314929
2021 MiR-144-3p-mediated dysregulation of EIF4G2 contributes to the development of hepatocellular carcinoma through the ERK pathway. Journal of experimental & clinical cancer research : CR 35 33526055
2015 Cleavage of DAP5 by coxsackievirus B3 2A protease facilitates viral replication and enhances apoptosis by altering translation of IRES-containing genes. Cell death and differentiation 33 26586572
2009 The translation initiation factor DAP5 is a regulator of cell survival during mitosis. Cell cycle (Georgetown, Tex.) 32 19158497
2008 Effects of pre or posttraining dorsal hippocampus D-AP5 injection on fear conditioning to tone, background, and foreground context. Hippocampus 32 18727044
2013 Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structure (London, England : 1993) 31 23478064
2022 Ribosomal leaky scanning through a translated uORF requires eIF4G2. Nucleic acids research 29 35018467
2022 DAP5 enables main ORF translation on mRNAs with structured and uORF-containing 5' leaders. Nature communications 24 36473845
2022 Eukaryotic translation initiation factor eIF4G2 opens novel paths for protein synthesis in development, apoptosis and cell differentiation. Cell proliferation 24 36547008
2012 NAT1/DAP5/p97 and atypical translational control in the Drosophila Circadian Oscillator. Genetics 24 22904033
2022 Specific mechanisms of translation initiation in higher eukaryotes: the eIF4G2 story. RNA (New York, N.Y.) 23 36517212
2017 Nanoparticle-mediated dual delivery of resveratrol and DAP5 ameliorates kidney ischemia/reperfusion injury by inhibiting cell apoptosis and inflammation. Oncotarget 23 28465474
2018 The 1980s: D-AP5, LTP and a Decade of NMDA Receptor Discoveries. Neurochemical research 22 30284673
2006 The transcripts of SFRP1,CEP63 and EIF4G2 genes are frequently downregulated in transitional cell carcinomas of the bladder. Oncology 21 16410684
2018 Regulation of Hypoxia-Inducible Factor 1α during Hypoxia by DAP5-Induced Translation of PHD2. Molecular and cellular biology 20 29530922
2020 MicroRNA-197 regulates chondrocyte proliferation, migration, and inflammation in pathogenesis of osteoarthritis by targeting EIF4G2. Bioscience reports 19 32880393
2022 DAP5 drives translation of specific mRNA targets with upstream ORFs in human embryonic stem cells. RNA (New York, N.Y.) 18 35961752
2019 eIF4G2 balances its own mRNA translation via a PCBP2-based feedback loop. RNA (New York, N.Y.) 18 31010886
1998 Effects of D-AP5 and NMDA microiontophoresis on associative learning in the barrel cortex of awake rats. Brain research 17 9630587
2007 Drosophila NAT1, a homolog of the vertebrate translational regulator NAT1/DAP5/p97, is required for embryonic germband extension and metamorphosis. Development, growth & differentiation 16 17716306
2017 Synergistic effect between D-AP5 and muscimol in the nucleus accumbens shell on memory consolidation deficit in adult male Wistar rats: An isobologram analysis. Neurobiology of learning and memory 15 28412304
2012 DAP5 ameliorates cisplatin-induced apoptosis of renal tubular cells. American journal of nephrology 15 22555068
1992 Cortically evoked excitatory synaptic transmission in the cat red nucleus is antagonised by D-AP5 but not by D-AP7. Brain research 15 1361408
2009 Enkephalin derivative, cyclo[Nepsilon,Nbeta-carbonyl-D-Lys2, Dap5] enkephalinamide (cUENK6), induces a highly potent antinociception in rats. Neuropeptides 14 19376576
2008 The crystal structure of the C-terminal DAP5/p97 domain sheds light on the molecular basis for its processing by caspase cleavage. Journal of molecular biology 14 18722383
2024 MicroRNA-877-5p promotes osteoblast differentiation by targeting EIF4G2 expression. Journal of orthopaedic surgery and research 13 38342889
2019 DAP5 increases axonal outgrowth of hippocampal neurons by enhancing the cap-independent translation of DSCR1.4 mRNA. Cell death & disease 13 30718468
2004 The cardiovascular and renal effects of a highly potent mu-opioid receptor agonist, cyclo[N epsilon,N beta-carbonyl-D-Lys2,Dap5]enkephalinamide. European journal of pharmacology 12 15288587
2019 The mammalian host protein DAP5 facilitates the initial round of translation of Coxsackievirus B3 RNA. The Journal of biological chemistry 11 31455634
2021 Bone Mesenchymal Stem Cells Promote Extracellular Matrix Remodeling of Degenerated Nucleus Pulposus Cells via the miR-101-3p/EIF4G2 Axis. Frontiers in bioengineering and biotechnology 10 34513803
2017 Role of CA1 GABAA and GABAB receptors on learning deficit induced by D-AP5 in passive avoidance step-through task. Brain research 10 29038006
2008 Death-associated protein 5 (DAP5/p97/NAT1) contributes to retinoic acid-induced granulocytic differentiation and arsenic trioxide-induced apoptosis in acute promyelocytic leukemia. Apoptosis : an international journal on programmed cell death 10 18491231
2024 MicroRNA-411-5p alleviates lipid deposition in metabolic dysfunction-associated steatotic liver disease by targeting the EIF4G2/FOXO3 axis. Cellular and molecular life sciences : CMLS 9 39261317
2021 Long non-coding RNA (LncRNA) SNHG7/ Eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) involves in the malignant events of ovarian cancer cells with paclitaxel resistant. Bioengineered 9 34709112
2023 Dissecting the roles of EIF4G homologs reveals DAP5 as a modifier of CGG repeat-associated toxicity in a Drosophila model of FXTAS. Neurobiology of disease 8 37352983
2022 Long non-coding RNA MCM3AP antisense RNA 1 silencing upregulates microRNA-24-3p to accelerate proliferation and migration of vascular endothelial cells in myocardial infarction rats by reducing EIF4G2. Cell cycle (Georgetown, Tex.) 8 35113004
2017 LRF inhibits p53 expression in colon cancer cells via modulating DAP5 activity. Cell biochemistry and function 8 28849590
2015 The influence of the new enkephalin derivative, cyclo[N(ε),N(β)-carbonyl-d-Lys(2),Dap(5)] enkephalinamide (cUENK6), on reinstatement of ethanol-induced conditioned place preference in rats. Physiology & behavior 8 25817357
2001 DAP-5 is involved in MycN/IFNgamma-induced apoptosis in human neuroblastoma cells. Cancer letters 8 11146231
2023 miR-379-5P INHIBITION ENHANCES INTESTINAL EPITHELIAL PROLIFERATION AND BARRIER FUNCTION RECOVERY AFTER ISCHEMIA/REPERFUSION BY TARGETING EIF4G2. Shock (Augusta, Ga.) 7 37646610
2020 lncRNA Eif4g2 improves palmitate-induced dysfunction of mouse β-cells via modulation of Nrf2 activation. Experimental cell research 7 32956705
2019 Amorphous silica nanoparticles induce tumorigenesis via regulating ATP5H/SOD1-related oxidative stress, oxidative phosphorylation and EIF4G2/PABPC1-associated translational initiation. PeerJ 6 30863671
2025 eIF3d and eIF4G2 mediate an alternative mechanism of cap-dependent but eIF4E-independent translation initiation. The Journal of biological chemistry 5 39971159
2023 Circ_0020123 promotes non-small cell lung cancer progression via miR-146a-5p mediated regulation of EIF4G2 expression. Thoracic cancer 5 37993106
2024 EIF4G2 Promotes Hepatocellular Carcinoma Progression via IRES-dependent PLEKHA1 Translation Regulation. Journal of proteome research 4 39213495
2023 The Roles of eIF4G2 in Leaky Scanning and Reinitiation on the Human Dual-Coding POLG mRNA. International journal of molecular sciences 4 38138978
2017 The role of CA3 GABAB receptors on anxiolytic-like behaviors and avoidance memory deficit induced by D-AP5 with respect to Ca2+ ions. Progress in neuro-psychopharmacology & biological psychiatry 4 28800869
2009 Crystallization and preliminary X-ray diffraction analysis of the MIF4G domain of DAP5. Acta crystallographica. Section F, Structural biology and crystallization communications 4 20057060
2023 Loss-of-function cancer-linked mutations in the EIF4G2 non-canonical translation initiation factor. Life science alliance 3 38129098
2024 Modeling the structure and DAP5-binding site of the FGF-9 5'-UTR RNA utilized in cap-independent translation. RNA (New York, N.Y.) 2 38866431
2009 N-Methyl-D-aspartate receptor antagonist d-AP5 prevents pertussis toxin-induced alterations in rat spinal cords by inhibiting increase in concentrations of spinal CSF excitatory amino acids and downregulation of glutamate transporters. Brain research bulletin 2 19463918
2025 A critical genetic interaction between Gemin3/Ddx20 and translation initiation factor NAT1/eIF4G2 drives development. Developmental biology 1 39924071
2025 Effects of the LINC00641/miR-323a-3p/EIF4G2 axis on behaviors and brain monoamine neurotransmitters in chronic unpredictable mild stress mice. Cell biology and toxicology 1 40293545
2025 Endothelial cells secrete small extracellular vesicles to promote neuron endoplasmic reticulum stress injury via miR-146a-5p/Eif4g2 axis in ischemic stroke. Journal of advanced research 1 40633838
2025 Targeting DAP5 Disrupts Alternate Mode of Translational Initiation in Tregs and Potentiates Antitumor Immunity. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 1 41457459
2026 eIF4G2-dependent translation restrains pancreatic cancer progression. bioRxiv : the preprint server for biology 0 41890118
2026 Cellular sensor DAP5 decodes Betacoronaviral NSP5 to drive virus-induced senescence. Frontiers in immunology 0 41924271
2026 Translation factor eIF4G2 directs CD8+ T cell lineage commitment by selectively enabling the IL-7 receptor response. iScience 0 41940334
2026 Acute restraint stress causes anxiety-related behaviors and neuronal degeneration in the CA1 and PFC, which are blocked by crocin and D-AP5. Journal of psychiatric research 0 41950713
2026 eIF4G2-mediated selective translation of chromatin regulators safeguards adult intestinal stem cell identity and differentiation. Cell stem cell 0 42066769
2026 eIF4G2-Dependent Translation Restrains Pancreatic Cancer Progression. Cancer research 0 42202060
2024 RNA modification-related EIF4G2 is an immunotherapy determinant in osteosarcoma: A single-cell sequencing analysis. Environmental toxicology 0 38578024
2023 Modeling the Structure and DAP5 Binding Site of a Cap-Independent Translational Enhancer mRNA. bioRxiv : the preprint server for biology 0 37333283
2012 Microinjection of NMDA-type glutamate receptor agonist NMDA and antagonist D-AP-5 into the central nucleus of the amygdale alters water intake rather than food intake. Nan fang yi ke da xue xue bao = Journal of Southern Medical University 0 22588904

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