Affinage

CYTH1

Cytohesin-1 · UniProt Q15438

Length
398 aa
Mass
46.4 kDa
Annotated
2026-04-28
40 papers in source corpus 26 papers cited in narrative 26 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

Cytohesin-1 (CYTH1) is an ARF-family guanine nucleotide exchange factor that couples PI 3-kinase–dependent membrane recruitment to integrin inside-out signaling and ARF-dependent cytoskeletal remodeling in leukocytes, Schwann cells, and hematopoietic progenitors. Its Sec7 domain catalyzes GDP-to-GTP exchange on ARF1 and ARF6, while its PH domain and adjacent polybasic c domain cooperatively bind PI(3,4,5)P3 to drive plasma membrane translocation; a three-nucleotide microexon splice variant in the PH domain switches phosphoinositide selectivity between PIP3 and PI(4,5)P2, thereby controlling subcellular localization and RTK-driven Arf6 activation during cell migration (PMID:9050849, PMID:9693361, PMID:30404949). Independently of GEF activity, cytohesin-1 directly binds the β2 integrin cytoplasmic tail to activate LFA-1 and CR3, promoting leukocyte adhesion, transendothelial migration, phagocytosis, and hematopoietic stem cell bone marrow homing, whereas its Arf6-directed GEF activity is required for cell spreading, neutrophil oxidative burst, and Schwann cell myelination—the latter regulated by Fyn-mediated phosphorylation of Tyr-382 (PMID:8706128, PMID:10835351, PMID:20018626, PMID:23012656, PMID:27899358). PKCδ phosphorylation of the polybasic c domain promotes cytohesin-1 association with the actin cytoskeleton and maximal LFA-1–dependent adhesion (PMID:11438522).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1996 High

    The discovery that cytohesin-1 directly binds the β2 integrin cytoplasmic tail and promotes LFA-1-dependent adhesion established the first molecular link between a Sec7-domain protein and integrin inside-out signaling.

    Evidence Co-immunoprecipitation and domain-dissection overexpression/dominant-negative assays in Jurkat T cells

    PMID:8706128

    Open questions at the time
    • Mechanism by which cytohesin-1 binding alters integrin conformation was unknown
    • Whether GEF activity was required for adhesion was untested
  2. 1997 High

    Demonstration that cytohesin-1 catalyzes GDP-to-GTP exchange on ARF1 and ARF3 in vitro defined its enzymatic function as an ARF-GEF, raising the question of how GEF activity relates to integrin regulation.

    Evidence In vitro GEF assay with recombinant cytohesin-1 and purified ARFs using radiolabeled nucleotides

    PMID:9050849

    Open questions at the time
    • In vivo ARF substrate identity unresolved
    • Relationship between GEF activity and adhesion function unclear
  3. 1998 High

    Establishing that PI 3-kinase activation drives cytohesin-1 membrane recruitment through cooperative PH domain + polybasic c domain binding to PIP3 (Kd ~100 nM) placed the lipid second messenger upstream of cytohesin-1 and explained its stimulus-dependent translocation.

    Evidence BIAcore lipid-binding assays, domain deletion/chimera constructs, constitutively active PI 3-kinase overexpression, membrane fractionation in Jurkat cells

    PMID:9614087 PMID:9693361

    Open questions at the time
    • Whether other phosphoinositides could substitute in specific cellular contexts
    • Structural basis of PH–lipid cooperativity with the c domain
  4. 1998 High

    NMR structure of the Sec7 domain and systematic ARF1 mutagenesis mapped the GEF catalytic interface and showed that the Sec7 domain alone does not bind the β2 integrin tail, establishing that adhesion and GEF functions reside in separable domains.

    Evidence NMR spectroscopy, chemical shift perturbation mapping, structure-based and ARF1 chimeric mutagenesis

    PMID:10212218 PMID:9653114 PMID:9756891

    Open questions at the time
    • Full-length structure including PH and c domains not determined
    • How autoinhibition might regulate GEF activity
  5. 2000 High

    Separation-of-function experiments showed that β2 integrin binding activates LFA-1 epitope expression independently of GEF activity, while ARF-GEF activity is specifically required for post-adhesion cell spreading, resolving two mechanistically distinct outputs of cytohesin-1.

    Evidence GEF-dead mutant, β2 cytoplasmic domain mutants, activation epitope antibodies, spreading and adhesion assays

    PMID:10835351

    Open questions at the time
    • Which ARF substrate mediates spreading was not identified in this study
    • How GEF-independent integrin activation is transmitted structurally
  6. 2001 High

    Identification that PKCδ phosphorylates the polybasic c domain and that this modification drives actin cytoskeleton association and maximal LFA-1 adhesion revealed a post-translational regulatory mechanism for cytohesin-1 localization and function.

    Evidence In vitro PKCδ kinase assay, phorbol ester stimulation in vivo, actin co-sedimentation, adhesion assays

    PMID:11438522

    Open questions at the time
    • Precise phosphorylation site(s) not fully mapped
    • Whether PKCδ phosphorylation affects GEF activity toward ARFs
  7. 2001 High

    Dissection of leukocyte transendothelial migration showed that PH domain–mediated arrest is GEF-independent, while shape change and chemotaxis require both GEF activity and β2 interaction, with ARF6 (not ARF1) identified as the relevant GEF substrate during diapedesis.

    Evidence Multiple dominant-negative/mutant constructs in flow-based adhesion and transendothelial chemotaxis assays

    PMID:11747824

    Open questions at the time
    • Downstream effectors of Arf6 in diapedesis not identified
    • In vivo validation in animal models absent
  8. 2005 Medium

    The finding that cytohesin-1 associates with CR3 (Mac-1) and mediates CD14-TLR2-PI3K–driven CR3 activation for mycobacterial phagocytosis extended its integrin-activating role beyond LFA-1 to complement receptors in innate immunity.

    Evidence siRNA knockdown, blocking antibodies, PI3K inhibitors, co-immunoprecipitation of CR3 and cytohesin-1, phagocytosis assay

    PMID:15778383

    Open questions at the time
    • Direct binding site on CR3 not mapped
    • Single lab, awaits independent confirmation
  9. 2009 High

    Pharmacological inhibition and knockdown in neutrophils demonstrated that cytohesin-1 selectively activates Arf6 downstream of fMLF receptor signaling to drive PLD activation, superoxide production, and granule secretion, linking it to the oxidative burst.

    Evidence SecinH3 inhibition, siRNA knockdown and overexpression in PLB-985 neutrophil-like cells, Arf6/Arf1 activation assays, NADPH oxidase assay

    PMID:20018626

    Open questions at the time
    • Specificity of SecinH3 among cytohesin family members
    • Whether cytohesin-1 is redundant with other cytohesins in neutrophils
  10. 2012 High

    Discovery that Fyn phosphorylates cytohesin-1 at Y382 and that this event is required for Arf6 activation and Schwann cell myelination—validated in both knockout and phospho-deficient transgenic mice—established a non-immune developmental function for cytohesin-1.

    Evidence In vitro Fyn kinase assay, cytohesin-1 KO mice, Y382F knock-in transgenic mice, electron microscopy of myelin, Arf6 activation assay

    PMID:23012656 PMID:23636892

    Open questions at the time
    • Downstream Arf6 effectors in myelination unidentified
    • Whether Y382 phosphorylation affects integrin-binding function
  11. 2016 High

    An RNAi screen followed by in vivo transplantation demonstrated that cytohesin-1 is essential for integrin β1 and β2 activation in human hematopoietic stem/progenitor cells and for their bone marrow homing and engraftment, broadening its role to stem cell biology.

    Evidence RNA interference, integrin activation and adhesion assays, HSPC transplantation into immunodeficient mice, intravital microscopy

    PMID:27899358

    Open questions at the time
    • Which cytohesin-1 domain mediates β1 integrin activation is unknown
    • Redundancy with other cytohesins in HSPCs not assessed
  12. 2018 High

    Characterization of a PH domain microexon splice variant showed that a single-residue insertion switches phosphoinositide selectivity from PIP3 to PI(4,5)P2, controlling subcellular localization and coupling the PIP3-binding isoform specifically to HGF/Met-induced Arf6 activation and cancer cell migration.

    Evidence Isoform-specific expression, phosphoinositide-binding assays, live-cell imaging, Met receptor and Arf6 activation assays, migration assays

    PMID:30404949

    Open questions at the time
    • Splicing regulators controlling microexon inclusion unknown
    • In vivo relevance to tumor metastasis not tested
    • Whether isoform switching also governs immune cell functions

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the full-length autoinhibited structure of cytohesin-1, the identity of Arf6 effectors mediating myelination and cell spreading, redundancy versus specificity among the four cytohesin family members in vivo, and whether isoform-specific lipid selectivity differentially regulates immune versus developmental functions.
  • No full-length structure available
  • Arf6 effectors downstream of cytohesin-1 in myelination and spreading not identified
  • Systematic in vivo comparison of cytohesin family members lacking

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 7 GO:0008289 lipid binding 3 GO:0060090 molecular adaptor activity 3 GO:0008092 cytoskeletal protein binding 1
Localization
GO:0005886 plasma membrane 3 GO:0005829 cytosol 2 GO:0005794 Golgi apparatus 1 GO:0005856 cytoskeleton 1
Pathway
R-HSA-168256 Immune System 7 R-HSA-162582 Signal Transduction 6 R-HSA-1500931 Cell-Cell communication 4 R-HSA-1266738 Developmental Biology 3
Partners
ARF1ARF6FYNITGAMITGB2PHLDB1PRKCQ

Evidence

Reading pass · 26 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1996 Cytohesin-1 specifically interacts with the intracellular portion of the integrin β2 chain (CD18) and overexpression of either full-length cytohesin-1 or its SEC7 domain induces β2 integrin-dependent binding of Jurkat cells to ICAM-1, while expression of the isolated PH domain inhibits T cell receptor-stimulated adhesion. Co-immunoprecipitation, overexpression/dominant-negative functional assay in Jurkat cells Cell High 8706128
1997 Cytohesin-1 functions as a guanine nucleotide-exchange protein (GEP) for ADP-ribosylation factors (ARFs), accelerating GDP-to-GTP exchange on ARF1 and ARF3, and increasing ARF1 binding to a Golgi fraction in a brefeldin A-insensitive manner. In vitro GEF assay with recombinant cytohesin-1 and purified bovine brain ARF; [35S]GTPγS and [3H]GDP binding assays Proceedings of the National Academy of Sciences of the United States of America High 9050849
1998 PI 3-kinase activation is sufficient to induce membrane recruitment of cytohesin-1 and activate β2 integrin-dependent adhesion; this is abrogated by overexpression of the isolated cytohesin-1 PH domain, placing PI 3-kinase upstream of cytohesin-1 in the inside-out signaling pathway. Constitutively active PI 3-kinase overexpression, dominant-negative PH domain constructs, membrane fractionation, adhesion assay in Jurkat cells The Journal of biological chemistry High 9614087
1998 Both the PH domain and the adjacent carboxyl-terminal polybasic (c) domain of cytohesin-1 are required cooperatively for high-affinity plasma membrane association (Kd ~100 nM for PtdIns(3,4,5)P3) and for biological inhibition of T cell adhesion; the isolated PH domain has substantially lower affinity (~2–3 µM). Biosensor (BIAcore) lipid-binding measurements, domain deletion/chimera overexpression, functional adhesion assays in Jurkat cells Molecular biology of the cell High 9693361
1998 The solution structure of the cytohesin-1 Sec7 domain (10 α-helices) was determined by NMR; ARF1 binding occurs through a large surface on the C-terminal subdomain involving hydrophobic and polar residues; structure-based mutagenesis identified residues critical for ARF binding and nucleotide exchange; the Sec7 domain does not interact with the β2 integrin cytoplasmic domain in solution. NMR spectroscopy, 1H-15N and 1H-13C chemical shift perturbation mapping, structure-based mutagenesis Proceedings of the National Academy of Sciences of the United States of America High 9653114
1998 Cytohesin-1 and its isolated Sec7 domain catalyze guanine nucleotide exchange on ARF1, ARF3, yeast ARF1–3, and ARD1 but not on ARL proteins; the Sec7 domain alone is broader in substrate specificity than full-length cytohesin-1, indicating that regions outside the Sec7 domain impose ARF specificity. In vitro GEF assay with recombinant proteins and [35S]GTPγS binding The Journal of biological chemistry High 9756891
1999 EGF- and NGF-stimulated PI 3-kinase activity drives rapid translocation of cytohesin-1 from cytosol to the plasma membrane in PC12 cells via PH domain binding to PIP3; the PH domain binds the inositol head group of PIP3 (IP4) with >200-fold higher affinity than PIP2 or PI(3,4)P2 in vitro. GFP-fusion live-cell confocal microscopy, wortmannin/LY294002 PI 3-kinase inhibition, in vitro IP4 binding assay Journal of cell science High 10341214
1999 LPS-induced monocyte adherence to ICAM-1 involves CD14, Rho, PI 3-kinase, and cytohesin-1; cytohesin-1 antisense knockdown attenuates LPS-induced adhesion, placing cytohesin-1 downstream of PI 3-kinase in inside-out LFA-1 activation. Antisense knockdown, PI 3-kinase inhibitors (LY294002, wortmannin), dominant-negative PI 3-kinase transfection, Clostridium difficile toxin B, adhesion assay The Journal of biological chemistry Medium 9873050
1999 Multiple structural elements of ARF1 are required for functional interaction with cytohesin-1: a non-specific N-terminal sequence (residues 1–13), residues 28–50 (including Lys-38 in switch 1), and the C-terminal region; the Sec7 domain alone has broader ARF recognition than full-length cytohesin-1. ARF1/ARL1 chimeric protein GEF assay, site-directed mutagenesis, [35S]GTPγS binding The Journal of biological chemistry High 10212218
2000 Cytohesin-1 regulates β2 integrin-mediated cell adhesion through two dissectable mechanisms: (1) direct interaction with the LFA-1 β2 cytoplasmic domain to induce LFA-1 activation epitope expression (GEF-independent), and (2) ARF-GEF activity required for cell spreading on ICAM-1; a GEF-dead mutant fails to support spreading. β2 cytoplasmic domain mutational analysis, activation epitope antibody staining, in vitro GEF assay, cell spreading and adhesion assays The EMBO journal High 10835351
2000 PtdIns(3,4,5)P3 determines the substrate specificity of cytohesin-1 GEF activity: it suppresses GEF activity toward ARF6 but enhances it toward ARF1, revealing lipid-dependent substrate switching. In vitro GEF assay with mammalian ARF-Ig chimeras, immunoprecipitation, phosphoinositide supplementation European journal of biochemistry Medium 10848997
2000 Cytohesin-1 specifically activates ARD1 (a 64-kDa GTPase with C-terminal ARF domain) as a GEF substrate in a manner requiring residue 30 in the Sec7 domain; ARD1 and cytohesin-1 partially colocalize in transfected COS-7 cells, consistent with a role in vesicular trafficking. Yeast two-hybrid screen, in vitro GEF assay, site-directed mutagenesis of Sec7 domain, confocal fluorescence microscopy of co-transfected cells The Journal of biological chemistry Medium 10748148
2000 B2-1 (cytohesin-1) localizes to the Golgi complex rather than the plasma membrane; this localization is disrupted by brefeldin A, indicating dependence on ARF activity at the Golgi; overexpression causes partial Golgi dispersion. Immunofluorescence, GFP-fusion confocal microscopy, brefeldin A treatment in transfected cells Experimental cell research Medium 10772823
2001 The kaposin A protein of human herpesvirus 8 directly interacts with cytohesin-1 and recruits it to the membrane; kaposin A stimulates cytohesin-1-dependent GTP loading of myristoylated ARF1 in vitro; a GEF-dead cytohesin-1 mutant (E157K) reverts kaposin A-induced focus formation, stress fiber dissolution, and ERK-1/2 MAP kinase activation. Direct protein interaction assay, in vitro liposome-reconstituted GEF assay with myristoylated ARF1, dominant-negative mutant rescue, ERK-1/2 activation assay Molecular cell High 11336706
2001 Protein kinase Cδ phosphorylates a serine/threonine motif in the polybasic c domain of cytohesin-1 in vitro, and phorbol ester stimulation induces the same phosphorylation in vivo; phosphorylated cytohesin-1 associates with the actin cytoskeleton, and this phosphorylation is required for maximal LFA-1-mediated adhesion. In vitro PKCδ kinase assay, phorbol ester stimulation, actin cytoskeleton co-sedimentation, functional adhesion assay The Journal of biological chemistry High 11438522
2001 Cytohesin-1 PH domain (GEF-independent) mediates leukocyte arrest on cytokine-activated endothelium triggered by chemokines, whereas both GEF activity and LFA-1/cytohesin-1 interaction are required for cell shape change and transendothelial chemotaxis; ARF6 (but not ARF1) is identified as the downstream GEF target in chemotaxis. Overexpression of dominant-negative PH domain construct, GEF-dead mutant, β2 mutant interrupting cytohesin-1 interaction, flow-based leukocyte adhesion assay, transendothelial chemotaxis assay Current biology : CB High 11747824
2002 Cybr (cytohesin binder and regulator) physically interacts with cytohesin-1 through coiled-coil domain interactions and enhances cytohesin-1-mediated acceleration of GTPγS binding to ARF in vitro. Co-immunoprecipitation of overexpressed proteins from 293T cells, in vitro GEF assay with Cybr Proceedings of the National Academy of Sciences of the United States of America Medium 11867758
2003 LFA-1 activation induces phosphorylation of the β2 integrin chain, release of JAB-1, and ERK1/2 signaling through cytohesin-1; dominant-negative cytohesin-1 inhibits IL-2 production and T helper type 1 differentiation, establishing cytohesin-1 as a mediator of LFA-1 co-stimulatory signaling. Intracellular phosphoprotein staining with 13-dimensional flow cytometry, dominant-negative overexpression, cytokine production assays Nature immunology Medium 14528303
2005 Cytohesin-1 promotes phagocytosis of M. bovis BCG via a CD14-TLR2-PI3K-cytohesin-1 inside-out signaling axis that activates complement receptor 3 (CR3/Mac-1); cytohesin-1 knockdown abrogates CD14-regulated CR3-dependent BCG internalization and cytohesin-1 physically associates with CR3. siRNA knockdown, blocking antibodies, PI3K inhibitors, flow cytometry phagocytosis assay, co-immunoprecipitation of CR3 and cytohesin-1 Journal of immunology Medium 15778383
2009 Cytohesin-1 activates Arf6 (but not Arf1) in human neutrophils stimulated with fMLF, linking fMLF receptor signaling to phospholipase D activation, superoxide production, and granule secretion; cytohesin-1 inhibition with SecinH3 or siRNA knockdown selectively suppresses Arf6 activation and downstream oxidative burst. SecinH3 pharmacological inhibition, cytohesin-1 siRNA knockdown and stable overexpression in PLB-985 cells, Arf6/Arf1 activation assays, PLD activity assay, NADPH oxidase assay, granule marker surface expression Journal of immunology High 20018626
2011 Cytohesin-1 restrains Mac-1 (αMβ2) activation in neutrophils; inhibition or knockdown of cytohesin-1 increases Mac-1 conformational activation, cell adhesion to fibrinogen, phagocytosis of zymosan, and chemotaxis, while overexpression has opposite effects; the mechanism involves crosstalk between FPRL-1 receptor and Mac-1 through cytohesin-1. SecinH3 inhibition, siRNA knockdown, stable overexpression, Mac-1 activation epitope antibody (CBRM1/5), adhesion assay, phagocytosis assay, transwell chemotaxis assay Journal of leukocyte biology Medium 21233413
2012 Fyn (Src-family kinase) phosphorylates tyrosine 382 of cytohesin-1 in Schwann cells; this phosphorylation is required for cytohesin-1-mediated Arf6 activation and myelination; transgenic mice expressing phosphorylation-deficient Y382F cytohesin-1 in Schwann cells display delayed and reduced myelination similar to cytohesin-1 knockout mice. In vitro Fyn kinase assay, cytohesin-1 Y382F knock-in transgenic mice, cytohesin-1 knockout mice, electron microscopy of myelin thickness, Arf6 activation assay Science signaling High 23012656
2013 Cytohesin-1 and Arf6 are required for migration of primary Schwann cells toward neuronal conditioned medium and individual growth factors; knockdown of cytohesin-1 or Arf6, SecinH3 treatment, or cytohesin-1 knockout inhibits migration; a Tyr-382 phosphorylation-deficient mutant fails to rescue blunted migration. siRNA knockdown, cytohesin-1 knockout, SecinH3 pharmacological inhibition, rescue with siRNA-resistant WT or Y382F mutant, Schwann cell migration assay Cellular signalling High 23517829
2013 Schwann cell-specific overexpression of wild-type cytohesin-1 in transgenic mice results in enhanced myelin thickness in PNS nerves and downstream Arf6 activation, confirming that cytohesin-1-Arf6 signaling promotes myelination in vivo. Schwann cell-specific transgenic mice, electron microscopy measurement of myelin thickness, Arf6 activation assay Journal of molecular neuroscience High 23636892
2016 CYTH1 knockdown disrupts adhesion of human hematopoietic stem and progenitor cells (HSPCs) to stromal cells, fibronectin, and ICAM-1 by impairing integrin β1 and β2 activation; transplantation of knockdown cells into immunodeficient mice results in reduced bone marrow homing and long-term engraftment, demonstrated by intravital microscopy. RNA interference screen, integrin activation assays, adhesion assays, HSPC transplantation into immunodeficient mice, intravital microscopy Blood High 27899358
2018 Alternative splicing of a three-nucleotide microexon in the PH domain of cytohesin-1 generates isoforms with differential phosphoinositide binding: the diglycine isoform preferentially binds PIP3 and localizes to the leading edge, while the triglycine isoform binds PI(4,5)P2 and localizes to the plasma membrane; the PI(3,4,5)P3-binding diglycine isoform specifically mediates HGF/Met receptor-induced Arf6 activation and cancer cell migration. Isoform-specific expression, phosphoinositide-binding assays, live-cell GFP/mCherry localization imaging, Met receptor activation, Arf6 activation assay, migration assay The Journal of cell biology High 30404949

Source papers

Stage 0 corpus · 40 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1996 Alpha L beta 2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1, a cytoplasmic regulatory molecule. Cell 384 8706128
2003 Leukocyte functional antigen 1 lowers T cell activation thresholds and signaling through cytohesin-1 and Jun-activating binding protein 1. Nature immunology 170 14528303
1997 Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for ADP-ribosylation factor. Proceedings of the National Academy of Sciences of the United States of America 131 9050849
1998 Phosphoinositide 3-OH kinase activates the beta2 integrin adhesion pathway and induces membrane recruitment of cytohesin-1. The Journal of biological chemistry 119 9614087
2000 Cytohesin-1 regulates beta-2 integrin-mediated adhesion through both ARF-GEF function and interaction with LFA-1. The EMBO journal 111 10835351
2001 Signaling by human herpesvirus 8 kaposin A through direct membrane recruitment of cytohesin-1. Molecular cell 92 11336706
2000 Similarities in function and gene structure of cytohesin-4 and cytohesin-1, guanine nucleotide-exchange proteins for ADP-ribosylation factors. The Journal of biological chemistry 79 10652308
1998 The PH domain and the polybasic c domain of cytohesin-1 cooperate specifically in plasma membrane association and cellular function. Molecular biology of the cell 79 9693361
1999 Monocyte adherence induced by lipopolysaccharide involves CD14, LFA-1, and cytohesin-1. Regulation by Rho and phosphatidylinositol 3-kinase. The Journal of biological chemistry 78 9873050
2005 Cross-talk between CD14 and complement receptor 3 promotes phagocytosis of mycobacteria: regulation by phosphatidylinositol 3-kinase and cytohesin-1. Journal of immunology (Baltimore, Md. : 1950) 74 15778383
1999 EGF-and NGF-stimulated translocation of cytohesin-1 to the plasma membrane of PC12 cells requires PI 3-kinase activation and a functional cytohesin-1 PH domain. Journal of cell science 56 10341214
2001 Cytohesin-1 is a dynamic regulator of distinct LFA-1 functions in leukocyte arrest and transmigration triggered by chemokines. Current biology : CB 47 11747824
2012 Phosphorylation of cytohesin-1 by Fyn is required for initiation of myelination and the extent of myelination during development. Science signaling 44 23012656
2002 Cybr, a cytokine-inducible protein that binds cytohesin-1 and regulates its activity. Proceedings of the National Academy of Sciences of the United States of America 44 11867758
1998 Guanine nucleotide exchange on ADP-ribosylation factors catalyzed by cytohesin-1 and its Sec7 domain. The Journal of biological chemistry 41 9756891
1998 Solution structure of the cytohesin-1 (B2-1) Sec7 domain and its interaction with the GTPase ADP ribosylation factor 1. Proceedings of the National Academy of Sciences of the United States of America 34 9653114
2001 Actin cytoskeletal association of cytohesin-1 is regulated by specific phosphorylation of its carboxyl-terminal polybasic domain. The Journal of biological chemistry 31 11438522
2009 Cytohesin-1 regulates the Arf6-phospholipase D signaling axis in human neutrophils: impact on superoxide anion production and secretion. Journal of immunology (Baltimore, Md. : 1950) 26 20018626
2011 Cytohesin-1 regulates fMLF-mediated activation and functions of the β2 integrin Mac-1 in human neutrophils. Journal of leukocyte biology 25 21233413
2018 HGF-induced migration depends on the PI(3,4,5)P3-binding microexon-spliced variant of the Arf6 exchange factor cytohesin-1. The Journal of cell biology 23 30404949
2000 Phosphoinositides determine specificity of the guanine-nucleotide exchange activity of cytohesin-1 for ADP-ribosylation factors derived from a mammalian expression system. European journal of biochemistry 22 10848997
2011 Cytohesin-1 regulates human blood neutrophil adhesion to endothelial cells through β2 integrin activation. Molecular immunology 20 21511340
2016 Cytohesin 1 regulates homing and engraftment of human hematopoietic stem and progenitor cells. Blood 18 27899358
2023 Separation and Purification of Antioxidant Peptide from Fermented Whey Protein by Lactobacillus rhamnosus B2-1. Food science of animal resources 14 36789200
2000 Specific functional interaction of human cytohesin-1 and ADP-ribosylation factor domain protein (ARD1). The Journal of biological chemistry 14 10748148
2011 Inhibition of cytohesin-1 by siRNA leads to reduced IGFR signaling in prostate cancer. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 12 21670894
1999 Structural elements of ADP-ribosylation factor 1 required for functional interaction with cytohesin-1. The Journal of biological chemistry 10 10212218
2023 Targeting cytohesin-1 suppresses acute myeloid leukemia progression and overcomes resistance to ABT-199. Acta pharmacologica Sinica 9 37644132
2024 Novel Antioxidant Peptides from Fermented Whey Protein by Lactobacillus rhamnosus B2-1: Separation and Identification by in Vitro and in Silico Approaches. Journal of agricultural and food chemistry 8 39392363
2014 Identification of the mulberry genes involved in ethylene biosynthesis and signaling pathways and the expression of MaERF-B2-1 and MaERF-B2-2 in the response to flooding stress. Functional & integrative genomics 8 25231943
2013 In vivo expression of the Arf6 Guanine-nucleotide exchange factor cytohesin-1 in mice exhibits enhanced myelin thickness in nerves. Journal of molecular neuroscience : MN 8 23636892
2002 ARNO but not cytohesin-1 translocation is phosphatidylinositol 3-kinase-dependent in HL-60 cells. Journal of leukocyte biology 8 11927660
2001 Characterization of cytohesin-1 monoclonal antibodies: expression in neutrophils and during granulocytic maturation of HL-60 cells. Journal of immunological methods 8 11226470
2000 B2-1, a Sec7- and pleckstrin homology domain-containing protein, localizes to the Golgi complex. Experimental cell research 8 10772823
1993 Assignment of human B2-1 gene (D17S811E) to chromosome 17qter by PCR analysis of somatic cell hybrids and fluorescence in situ hybridization. Cytogenetics and cell genetics 8 8449036
2013 Signaling through Arf6 guanine-nucleotide exchange factor cytohesin-1 regulates migration in Schwann cells. Cellular signalling 7 23517829
2000 Cloning and characterization of the promoter of murine cytohesin-1 gene. Biochimica et biophysica acta 4 10978522
2005 Cytohesin-1: structure, function, and ARF activation. Methods in enzymology 3 16413269
2017 Data on the effect of knockout of cytohesin-1 in myelination-related protein kinase signaling. Data in brief 1 29022003
2024 A natural loss-of-function deletion of the cytohesin 1 (Cyth1) gene in BALB/cByJ mice does not impact cardiomyocyte polyploidy. Scientific reports 0 38858421