Affinage

CRYAB

Alpha-crystallin B chain · UniProt P02511

Length
175 aa
Mass
20.2 kDa
Annotated
2026-04-28
100 papers in source corpus 25 papers cited in narrative 25 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CRYAB (αB-crystallin/HSPB5) is an ATP-independent small heat shock protein that functions as a stress-responsive molecular chaperone, cytoskeletal stabilizer, and modulator of multiple signaling pathways in the lens, heart, brain, and other tissues. Its chaperone holdase activity is governed by oligomeric transitions: under basal conditions, N-terminal regions are sequestered within polydisperse oligomers, but stress cues such as acidosis destabilize the α-crystallin domain dimer interface via a conserved histidine (His-104), shifting the equilibrium toward monomers and expanded oligomers with exposed client-binding surfaces; disease mutations R120G and D109H lock the protein in a constitutively activated, coaggregation-prone state that causes cataract, myofibrillar myopathy, and cardiomyopathy (PMID:25962097, PMID:40377988, PMID:30567736). Beyond chaperoning, CRYAB stabilizes cytoskeletal integrity by binding actin and the actin crosslinker filamin at Z-bands in muscle, associates with cytochrome c and VDAC at mitochondria to suppress apoptosis and maintain oxidative phosphorylation, inhibits NF-κB signaling by blocking IKK complex formation, stabilizes β-catenin and E-cadherin at adherens junctions to modulate Wnt signaling, and facilitates p53 degradation through an Fbx4/SCF ubiquitin ligase complex (PMID:25715399, PMID:39561005, PMID:31481750, PMID:22158051, PMID:19343786). Phosphorylation at Ser45 and Ser59 regulates both chaperone client engagement and exosomal secretion, and is required for CRYAB's dendrite-protective function in neurons (PMID:26620801, PMID:33220080).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2006 High

    The R120G mutation was shown to produce toxic amyloid-like oligomers that impair the ubiquitin-proteasome system, establishing that disease pathogenesis involves gain-of-toxic-function aggregation rather than simple loss of chaperone activity.

    Evidence Recombinant R120G CryAB analyzed by native PAGE and anti-oligomer antibody in cardiomyocytes

    PMID:17092938

    Open questions at the time
    • Structure of R120G toxic oligomers not resolved at atomic level
    • Whether toxic oligomers form in vivo in patient tissue not directly shown
  2. 2007 High

    Genetic rescue experiments in DKO mice separated CRYAB's cardioprotective role (maintaining cytoskeletal/contractile integrity) from HSPB2's role (energetic homeostasis), establishing non-redundant functions among closely related sHSPs in the heart.

    Evidence DKO, CryAB-transgenic rescue mice subjected to ischemia/reperfusion with 31P NMR spectroscopy

    PMID:17846079

    Open questions at the time
    • Direct cytoskeletal client identity in cardiac myofibrils not fully mapped
    • Whether CRYAB and HSPB2 cooperate in any shared client remains untested
  3. 2009 Medium

    Discovery that CRYAB interacts with Fbx4 and p53 and that CRYAB-null cells accumulate p53 revealed an unexpected role as a cofactor for SCF-Fbx4-mediated proteasomal degradation of p53, linking the chaperone to cell fate decisions beyond proteostasis.

    Evidence Endogenous co-IP and p53 stability assays in αBcry−/− MEFs

    PMID:19343786

    Open questions at the time
    • Whether CRYAB directly bridges Fbx4 to p53 or acts allosterically is unresolved
    • Independent replication in a second lab needed
  4. 2011 High

    CRYAB was found to physically associate with E-cadherin and β-catenin at adherens junctions, stabilizing membrane β-catenin and suppressing Wnt-target gene expression, thus extending CRYAB's roles from proteostasis to cell adhesion and tumor suppression.

    Evidence Co-IP with recombinant CRYAB, domain mapping, in vivo tumor formation assay in nude mice

    PMID:22158051

    Open questions at the time
    • Stoichiometry of CRYAB in the cadherin-catenin complex unknown
    • Whether chaperone activity is required for this interaction not tested
  5. 2013 Medium

    Analysis of CRYAB-deficient endothelial cells revealed that CRYAB enhances TNF-α-driven NF-κB activation by facilitating IκB degradation, while a subsequent study showed CRYAB suppresses NF-κB by blocking IKK complex formation in intestinal epithelium, establishing that CRYAB's effect on NF-κB is cell-type dependent.

    Evidence CRYAB-deficient mouse endothelial cells (2013) and CRISPR knockout intestinal epithelial cells with in vivo colitis models (2019)

    PMID:23929007 PMID:31481750

    Open questions at the time
    • Molecular basis for opposing NF-κB effects in different cell types not determined
    • Whether CRYAB directly binds IKKβ or an upstream scaffold remains unclear
  6. 2015 High

    The structural mechanism of stress-activated chaperone function was elucidated: acidosis destabilizes the ACD dimer interface at His-104, shifting CRYAB from sequestered oligomers to expanded, active states with exposed client-binding surfaces, revealing a pH-sensing switch.

    Evidence In vitro mutagenesis of His-104 with biophysical oligomer characterization and chaperone activity assays

    PMID:25962097

    Open questions at the time
    • Whether His-104-mediated activation occurs during physiological ischemic acidosis in vivo not shown
    • Atomic structure of the activated oligomer not determined
  7. 2015 High

    CRYAB was shown to localize at Z-bands and interact with filamin (Cheerio) in muscle, with loss-of-function causing myonuclear clustering and sarcomeric disorganization, establishing a direct structural role in myofibrillar architecture beyond general chaperone function.

    Evidence Co-IP/mass spectrometry plus genetic loss-of-function in Drosophila larval muscle

    PMID:25715399

    Open questions at the time
    • Direct actin-binding site on mammalian CRYAB not mapped
    • Whether filamin interaction is conserved in human muscle not confirmed
  8. 2015 Medium

    Phosphorylation at Ser45/Ser59 was found to negatively regulate CRYAB's sorting into exosomes, while O-GlcNAcylation promotes exosomal packaging, revealing post-translational control of CRYAB's extracellular chaperone function.

    Evidence Phosphomimetic constructs with CD63/Rab27 colocalization and exosome biochemistry

    PMID:26620801

    Open questions at the time
    • Functional consequence of exosomal CRYAB on recipient cells not defined
    • In vivo relevance of exosomal secretion not tested
  9. 2018 High

    Kinetic dissection of client engagement showed that WT CRYAB acts transiently early in the aggregation pathway of destabilized clients, while phosphomimetic and disease variants also intervene at later stages, distinguishing baseline holdase activity from stress-activated modes.

    Evidence In vitro chaperone assays with defined α-lactalbumin aggregation pathway and multiple HSPB5 variants

    PMID:30567736

    Open questions at the time
    • Identity of endogenous clients engaged at each stage in vivo unknown
    • Whether early vs. late intervention leads to different fates for the client not resolved
  10. 2020 Medium

    Phosphorylation at Ser45 and Ser59 was shown to be required for CRYAB's ability to promote dendritic complexity and protect against iron deficiency-induced dendritic rarefaction in hippocampal neurons, extending the functional importance of phosphoregulation to the nervous system.

    Evidence Phospho-mutant overexpression in cultured hippocampal neurons and in utero electroporation in mice

    PMID:33220080

    Open questions at the time
    • The downstream effector pathway by which phospho-CRYAB promotes dendritic complexity is unknown
    • Kinase identity responsible for neuronal CRYAB phosphorylation not identified in this context
  11. 2024 High

    The E105K mutation was shown to reduce CRYAB interaction with cytochrome c and VDAC and to impair OXPHOS complex assembly, causing retinal ganglion cell degeneration and optic atrophy, establishing a direct mitochondrial protective role for CRYAB beyond its cytoskeletal functions.

    Evidence Co-IP for cytochrome c/VDAC interaction, OXPHOS activity measurements, and retinal phenotyping in Cryab p.E105K knock-in mice

    PMID:39561005

    Open questions at the time
    • Whether CRYAB resides stably at the outer mitochondrial membrane or is transiently recruited is unknown
    • Mechanism by which CRYAB-VDAC interaction supports OXPHOS assembly not elucidated
  12. 2025 High

    HDX-MS and native MS resolved the structural basis of the nonactivated-to-activated transition: WT CRYAB reversibly sequesters its N-terminal regions within oligomers, while R120G and D109H mutants are constitutively activated with exposed N-terminal regions, unifying the structural and pathogenic mechanisms of multiple disease mutations.

    Evidence HDX-MS, native MS, and chaperone activity comparison of WT vs. R120G and D109H

    PMID:40377988

    Open questions at the time
    • Full atomic structure of the nonactivated oligomer remains undetermined
    • Whether therapeutic stabilization of the nonactivated state could reverse disease phenotypes is untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key open questions include: the full inventory of endogenous clients engaged by CRYAB's distinct activation states in vivo, the structural basis for cell-type-specific effects on NF-κB signaling, whether the mitochondrial protective function involves stable CRYAB residence at the outer membrane, and whether pharmacological stabilization of the nonactivated oligomer state is a viable therapeutic strategy.
  • No high-resolution structure of the full-length CRYAB oligomer in either activation state
  • Endogenous client repertoire in different tissues not systematically defined
  • Mechanism of cell-type-specific NF-κB modulation unresolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0044183 protein folding chaperone 4 GO:0098772 molecular function regulator activity 3 GO:0008092 cytoskeletal protein binding 2
Localization
GO:0005634 nucleus 2 GO:0005829 cytosol 2 GO:0005856 cytoskeleton 2 GO:0005739 mitochondrion 1 GO:0005886 plasma membrane 1 GO:0031410 cytoplasmic vesicle 1
Pathway
R-HSA-162582 Signal Transduction 6 R-HSA-8953897 Cellular responses to stimuli 4 R-HSA-168256 Immune System 3 R-HSA-392499 Metabolism of proteins 2 R-HSA-5357801 Programmed Cell Death 2
Partners
CDH1CTNNB1CYCSFBX4FTH1STAT3TP53VDAC1

Evidence

Reading pass · 25 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2015 HSPB5 (αB-crystallin) chaperone activity is regulated by a conserved histidine (His-104) at the ACD dimer interface: acidosis/low pH destabilizes the dimer interface via His-104, shifting HSPB5 from dimer to monomer and expanding oligomer states, which unleashes a cryptic holdase chaperone mode that reorganizes into structurally distinct client-bound complexes. In vitro biochemical assays, His-104 mutagenesis, biophysical characterization of oligomer states, chaperone activity assays eLife High 25962097
2025 Wild-type HSPB5 reversibly transitions between nonactivated (low chaperone activity, N-terminal regions sequestered within oligomers) and activated (high chaperone activity, N-terminal regions exposed) states. Disease mutants R120G and D109H are constitutively activated chaperones with exposed N-terminal regions, explaining their increased coaggregation propensity and early cataract. HDX-MS, native MS, chaperone activity assays, comparison of WT vs. disease mutants R120G and D109H Proceedings of the National Academy of Sciences of the United States of America High 40377988
2018 HSPB5 engages multiple aggregation states of a destabilized client (α-lactalbumin): under nonstress conditions WT HSPB5 acts transiently early in the aggregation pathway, while stress-mimicking phosphomimetic or disease mutants also intervene at later stages, indicating distinct client-interaction mechanisms between WT and activated states. In vitro chaperone activity assays with defined aggregation pathway of α-lactalbumin, comparison of WT and HSPB5 variants The Journal of biological chemistry High 30567736
2006 The R120G mutant CryAB protein forms toxic amyloid oligomers (~240–480 kDa) detectable by anti-oligomer antibody. HSP25 and HSP22 directly interrupt oligomer formation by R120G CryAB. This blockade recovers ubiquitin-proteasomal activity and cellular viability in cardiomyocytes. Recombinant protein production, native PAGE, anti-oligomer immunoreactivity, adenoviral transfection in cardiomyocytes, proteasome activity assay The Journal of biological chemistry High 17092938
2015 Drosophila CryAB (ortholog of human CRYAB) localizes at Z-bands and around myonuclei in larval muscles. It contains a conserved actin-binding domain and interacts with the actin crosslinker Cheerio (filamin) as shown by co-immunoprecipitation and mass spectrometry. CryAB attenuation causes myonuclear clustering and altered sarcomeric actin/Cheerio patterns, demonstrating a structural role in myofibrillar integrity. Mass spectrometry, co-immunoprecipitation, confocal microscopy, loss-of-function genetics, expression of DRM-associated R120G mutant in flies Development (Cambridge, England) High 25715399
2011 CRYAB physically associates with E-cadherin and β-catenin at the cadherin/catenin adherens junction via its α-crystallin core domain, thereby inhibiting E-cadherin cytoplasmic internalization and maintaining membrane β-catenin, which reduces downstream cyclin-D1 and c-Myc expression and suppresses NPC progression. Co-immunoprecipitation with ectopically expressed and recombinant CRYAB, domain mapping, tumor formation assay in nude mice, functional assays for invasion and EMT markers Oncogene High 22158051
2009 αB-crystallin interacts with Fbx4 ubiquitin ligase and p53 (both wild-type and mutant p53R175H) in a complex; αB-crystallin-deficient cells accumulate p53 protein due to impaired SCF-Fbx4-mediated degradation, demonstrating CRYAB's role as a cofactor in proteasomal p53 turnover. Co-immunoprecipitation (endogenous pulldown), MEF knockout cells, ectopic Fbx4 expression, p53 stability assays in hsf1−/− and αBcry−/− MEFs Journal of cellular biochemistry Medium 19343786
2019 CRYAB physically interacts with β-catenin and protects it from ubiquitination and proteasomal degradation, thereby stabilizing β-catenin and promoting Wnt signaling to enhance osteogenic differentiation of human BMSCs. Co-immunoprecipitation, in vitro ubiquitination assay, luciferase reporter assay, gain/loss-of-function in BMSCs, in vivo bone formation assay Cell proliferation Medium 31638302
2024 CRYAB interacts with ferritin heavy chain 1 (FTH1) and maintains FTH1 protein stability through the proteasome pathway in a lactylation-dependent manner, thereby suppressing ferroptosis and promoting osteogenic differentiation of human BMSCs. IP-MS identification of CRYAB-interacting proteins, co-immunoprecipitation, Western blotting, gain/loss-of-function, rescue experiments Aging Medium 38787373
2016 In astrocytes, CRYAB is upregulated and translocates to the nucleus, where it directly interacts with STAT3 (shown by co-immunoprecipitation) and inhibits STAT3 activation and DNA-binding activity, thereby suppressing neuroinflammatory cytokine production downstream of dopamine D2 receptor signaling. Co-immunoprecipitation, EMSA, Western blot, immunofluorescence, CRYAB and DRD2 knockdown, MCAO mouse model Journal of neuroinflammation Medium 27724964
2019 CRYAB inhibits IKKβ-mediated NF-κB signaling in intestinal epithelial cells by suppressing IKK complex formation, protecting mucosal barrier integrity. Administration of TAT-CRYAB fusion protein ameliorates DSS- and TNBS-induced colitis in mice. Lentivirus overexpression, CRISPR/Cas9 knockout, Western blot, in vivo colitis models, TAT-fusion protein administration Mucosal immunology Medium 31481750
2013 αB-crystallin enhances TNF-α-induced NF-κB signaling in endothelial cells: αB-crystallin-deficient endothelial cells show elevated IκB levels with incomplete IκB degradation upon TNF-α stimulation, resulting in reduced ICAM-1, VCAM-1, and E-selectin expression and impaired leukocyte-endothelial interactions in vivo. αB-crystallin-deficient mouse endothelial cells, TNF-α stimulation, Western blot, in vivo leukocyte rolling assay, adhesion molecule expression analysis Angiogenesis Medium 23929007
2010 HSPB1 selectively promotes ubiquitin-proteasome-mediated degradation of aggregate-prone CryAB mutants (R120G, 450delA, 464delCT); HSPB1 knockdown reduces solubility and increases aggregation of all CryAB mutants, while proteasome inhibitors and ubiquitin conjugate analysis confirm UPS-mediated clearance. HSPB1 overexpression/knockdown in H9c2 cells, proteasome inhibitor treatment, ubiquitin conjugate analysis, solubility fractionation Journal of molecular and cellular cardiology Medium 20863832
2015 Phosphorylation of CRYAB (at Ser45 and Ser59) negatively regulates its secretion via exosomes; phosphomimetic 3-SD CRYAB shows reduced colocalization with MVE/exosome markers CD63 and Rab27. O-GlcNAcylation of CRYAB promotes its packaging into exosomes. YFP-tagged phosphomimetic constructs, colocalization with CD63/Rab27 by immunofluorescence, exosome isolation, Western blot Biochimica et biophysica acta Medium 26620801
2007 CryAB and HSPB2 play distinct non-redundant roles in the heart: CryAB protects mechanical/contractile properties (cytoskeletal organization) while HSPB2 maintains energetic balance (ATP/PCr recovery). This was demonstrated using DKO mice, CryAB-transgenic rescue (DKO/mCryAB(Tg)), and 31P NMR spectroscopy under ischemia/reperfusion and inotropic stress. Genetic mouse models (DKO, mCryAB-Tg, DKO/mCryAB-Tg), isolated heart ischemia/reperfusion, inotropic stimulation, 31P NMR spectroscopy FASEB journal High 17846079
2018 CRYAB translocates from cytoplasm to nucleus under heat stress in cardiomyocytes and colocalizes with aggregated F-actin; CRYAB overexpression significantly reduces F-actin aggregation and prevents caspase-3-mediated apoptosis in H9C2 cells under heat stress. Stable overexpression cell lines, immunofluorescence for F-actin colocalization, apoptosis assays, caspase-3 expression analysis Cell stress & chaperones Medium 30246229
2007 BRG1 (SWI/SNF chromatin remodeling complex) activates the CRYAB promoter through a 30 bp element bound by HMGA1 proteins; HMGA1 binding sequences and HMGA1 itself are required for maximal BRG1-driven CRYAB transcriptional activation. Promoter-reporter assays, EMSA (in vitro and in vivo chromatin IP), deletion/mutation analysis of promoter element DNA and cell biology Medium 17723105
2024 CRYAB p.E105K mutation reduces interaction with cytochrome c and VDAC (voltage-dependent anion channel) as shown by co-immunoprecipitation, and promotes apoptosis and defective assembly/stability/activity of the oxidative phosphorylation system, causing retinal ganglion cell degeneration and optic atrophy in knock-in mice. Co-immunoprecipitation, Cryab p.E105K knock-in mouse model, apoptosis assays, OXPHOS activity measurements, retinal/RGC phenotypic analysis JCI insight High 39561005
2019 VP1-001 (an oxysterol) binds stereoselectively to native cryAB dimers as shown by differential scanning fluorimetry and microscale thermophoresis, and this direct binding is required for its ability to restore lens transparency in cryAB(R120G) mutant and aged wild-type mice; the non-binding enantiomer ent-VP1-001 has no effect. DSF, MST (microscale thermophoresis), in silico docking, topical treatment of mouse cataract models, TEM of lens morphology Investigative ophthalmology & visual science High 31369034
2020 Phosphorylation of HspB5 at S45 and S59 (but not S19) is essential for the dendritic complexity-enhancing and dendrite-protecting functions in hippocampal neurons; non-phosphorylatable HspB5-AAA mutant fails to increase dendritic complexity or protect against iron deficiency-induced dendritic rarefaction in vitro and in vivo. Overexpression of WT and phospho-mutants in cultured hippocampal neurons, Sholl analysis, in utero electroporation in mice, iron deficiency model Journal of neurochemistry Medium 33220080
2016 A phosphomimetic form of HspB5 increases trafficking to the plasma membrane, function, and stability of F508del-CFTR (the most common cystic fibrosis mutation), and these effects are enhanced by CFTR modulators VX-770/VX-809. F508del-CFTR expression in cell lines, phosphomimetic HspB5 constructs, plasma membrane fractionation, electrophysiology, Western blot International journal of molecular sciences Medium 32650630
2011 Molecular modeling and clinical data indicate that CRYAB residues D109 and R120 interact with each other during αB-crystallin dimerization; mutations at both residues (D109H and R120G) result in the same multisystemic disease phenotype (cataract, myofibrillar myopathy, cardiomyopathy), suggesting that impairment of αB-crystallin dimerization is a shared pathogenic mechanism. Molecular modeling, genotype-phenotype analysis across families with D109H and R120G mutations Neuromuscular disorders : NMD Low 21920752
2019 M2 macrophages upregulate CRYAB expression in NSCLC cells, which activates the ERK1/2/Fra-1/Slug signaling pathway to promote EMT and invasion; CRYAB knockdown suppresses these effects. Microfluidic co-culture device, iTRAQ proteomics, KD/KO cell experiments, animal metastasis models Cell death & disease Medium 31097690
2020 miR-450b-5p suppresses CRYAB expression; reduced CRYAB relieves inhibition of IKKβ-mediated canonical NF-κB signaling and reduces Akt1/mTOR-driven M2 macrophage polarization, thereby exacerbating hepatic ischemia/reperfusion injury. miRNA inhibitor treatment, Western blot, immunofluorescence, ELISA, in vivo mouse hepatic IRI model Cell death & disease Medium 32532961
2022 CRYAB negatively regulates TRIM33, which acts as an antifibrotic effector: HSPB5 (CRYAB) suppresses TRIM33 to limit TGF-β1/Smad3/Smad4 activation in cardiac fibroblasts, thereby reducing Ang II-induced myocardial fibrosis. Overexpression/knockdown in cardiac fibroblasts, Ang II mouse model, Masson staining, echocardiography Bioengineered Low 35333698

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2007 Hsp27 (HspB1) and alphaB-crystallin (HspB5) as therapeutic targets. FEBS letters 257 17467701
2012 Small heat shock proteins HSP27 (HspB1), αB-crystallin (HspB5) and HSP22 (HspB8) as regulators of cell death. The international journal of biochemistry & cell biology 229 22521623
2001 Alpha-B crystallin gene (CRYAB) mutation causes dominant congenital posterior polar cataract in humans. American journal of human genetics 204 11577372
2019 M2 macrophages promote NSCLC metastasis by upregulating CRYAB. Cell death & disease 112 31097690
2020 Inhibition of miR-450b-5p ameliorates hepatic ischemia/reperfusion injury via targeting CRYAB. Cell death & disease 103 32532961
2016 Sinomenine activates astrocytic dopamine D2 receptors and alleviates neuroinflammatory injury via the CRYAB/STAT3 pathway after ischemic stroke in mice. Journal of neuroinflammation 84 27724964
2014 Inflammatory cytokines, interleukin-1 beta and tumor necrosis factor-alpha, upregulated in glioblastoma multiforme, raise the levels of CRYAB in exosomes secreted by U373 glioma cells. Biochemical and biophysical research communications 83 25261722
2011 A novel CRYAB mutation resulting in multisystemic disease. Neuromuscular disorders : NMD 81 21920752
2007 Aging skeletal muscle shows a drastic increase in the small heat shock proteins alphaB-crystallin/HspB5 and cvHsp/HspB7. European journal of cell biology 78 17761354
2014 HspB1, HspB5 and HspB4 in Human Cancers: Potent Oncogenic Role of Some of Their Client Proteins. Cancers 77 24514166
2019 Progression of the role of CRYAB in signaling pathways and cancers. OncoTargets and therapy 73 31239701
2010 The p.G154S mutation of the alpha-B crystallin gene (CRYAB) causes late-onset distal myopathy. Neuromuscular disorders : NMD 70 20171888
2017 The novel αB-crystallin (CRYAB) mutation p.D109G causes restrictive cardiomyopathy. Human mutation 59 28493373
2015 A conserved histidine modulates HSPB5 structure to trigger chaperone activity in response to stress-related acidosis. eLife 59 25962097
2006 Interruption of CryAB-amyloid oligomer formation by HSP22. The Journal of biological chemistry 58 17092938
2011 Tumor suppressor Alpha B-crystallin (CRYAB) associates with the cadherin/catenin adherens junction and impairs NPC progression-associated properties. Oncogene 54 22158051
2018 Rosemary Reduces Heat Stress by Inducing CRYAB and HSP70 Expression in Broiler Chickens. Oxidative medicine and cellular longevity 53 30425783
2021 Salvianolic acid A relieves cognitive disorder after chronic cerebral ischemia: Involvement of Drd2/Cryab/NF-κB pathway. Pharmacological research 52 34800628
2018 The small heat shock proteins, especially HspB4 and HspB5 are promising protectants in neurodegenerative diseases. Neurochemistry international 52 29425965
2013 Protein interactomes of three stress inducible small heat shock proteins: HspB1, HspB5 and HspB8. International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group 52 23697380
2006 Identification of a CRYAB mutation associated with autosomal dominant posterior polar cataract in a Chinese family. Investigative ophthalmology & visual science 51 16877416
2016 A novel dominant D109A CRYAB mutation in a family with myofibrillar myopathy affects αB-crystallin structure. BBA clinical 46 27904835
2010 Selective degradation of aggregate-prone CryAB mutants by HSPB1 is mediated by ubiquitin-proteasome pathways. Journal of molecular and cellular cardiology 46 20863832
2015 Drosophila small heat shock protein CryAB ensures structural integrity of developing muscles, and proper muscle and heart performance. Development (Cambridge, England) 45 25715399
2017 The small heat shock proteins αB-crystallin (HSPB5) and Hsp27 (HSPB1) inhibit the intracellular aggregation of α-synuclein. Cell stress & chaperones 44 28337642
2020 MiRNA-671-5p Promotes prostate cancer development and metastasis by targeting NFIA/CRYAB axis. Cell death & disease 43 33144585
2019 Small heat shock protein CRYAB inhibits intestinal mucosal inflammatory responses and protects barrier integrity through suppressing IKKβ activity. Mucosal immunology 42 31481750
2010 Infantile onset myofibrillar myopathy due to recessive CRYAB mutations. Neuromuscular disorders : NMD 40 21130652
2009 Identification of a novel CRYAB mutation associated with autosomal recessive juvenile cataract in a Saudi family. Molecular vision 39 19461931
2018 BYD Ameliorates Oxidative Stress-Induced Myocardial Apoptosis in Heart Failure Post-Acute Myocardial Infarction via the P38 MAPK-CRYAB Signaling Pathway. Frontiers in physiology 38 29867551
2007 CRYAB and HSPB2 deficiency alters cardiac metabolism and paradoxically confers protection against myocardial ischemia in aging mice. American journal of physiology. Heart and circulatory physiology 38 17873008
2022 Senolysis induced by 25-hydroxycholesterol targets CRYAB in multiple cell types. iScience 36 35198901
2021 Presence and activation of pro-inflammatory macrophages are associated with CRYAB expression in vitro and after peripheral nerve injury. Journal of neuroinflammation 36 33761953
2009 A novel mutation in CRYAB associated with autosomal dominant congenital nuclear cataract in a Chinese family. Molecular vision 36 19597569
2015 Phosphorylation negatively regulates exosome mediated secretion of cryAB in glioma cells. Biochimica et biophysica acta 33 26620801
2015 Missense Mutations in CRYAB Are Liable for Recessive Congenital Cataracts. PloS one 31 26402864
2007 Unmasking different mechanical and energetic roles for the small heat shock proteins CryAB and HSPB2 using genetically modified mouse hearts. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 31 17846079
2018 CRYAB protects cardiomyocytes against heat stress by preventing caspase-mediated apoptosis and reducing F-actin aggregation. Cell stress & chaperones 30 30246229
2009 Heat shock factor 1 deficiency via its downstream target gene alphaB-crystallin (Hspb5) impairs p53 degradation. Journal of cellular biochemistry 30 19343786
2024 CRYAB suppresses ferroptosis and promotes osteogenic differentiation of human bone marrow stem cells via binding and stabilizing FTH1. Aging 29 38787373
2019 CRYAB promotes osteogenic differentiation of human bone marrow stem cells via stabilizing β-catenin and promoting the Wnt signalling. Cell proliferation 29 31638302
2005 Differential expression of alphaB-crystallin and Hsp27-1 in anaplastic thyroid carcinomas because of tumor-specific alphaB-crystallin gene (CRYAB) silencing. Cell stress & chaperones 29 16184762
2020 CRYAB inhibits migration and invasion of bladder cancer cells through the PI3K/AKT and ERK pathways. Japanese journal of clinical oncology 28 31829429
2011 Structural and functional specificity of small heat shock protein HspB1 and HspB4, two cellular partners of HspB5: role of the in vitro hetero-complex formation in chaperone activity. Biochimie 28 22210387
2021 Novel Secreted Protein of Mycoplasma bovis MbovP280 Induces Macrophage Apoptosis Through CRYAB. Frontiers in immunology 27 33659004
2020 Qishen Granule alleviates endoplasmic reticulum stress-induced myocardial apoptosis through IRE-1-CRYAB pathway in myocardial ischemia. Journal of ethnopharmacology 27 31945401
2017 Upregulation and phosphorylation of HspB1/Hsp25 and HspB5/αB-crystallin after transient middle cerebral artery occlusion in rats. Cell stress & chaperones 27 28425051
2016 Krüppel-like factor 4 promotes human osteosarcoma growth and metastasis via regulating CRYAB expression. Oncotarget 27 27105535
2019 Mechanism of Action of VP1-001 in cryAB(R120G)-Associated and Age-Related Cataracts. Investigative ophthalmology & visual science 26 31369034
2011 Cardioprotective effect of nicorandil, a mitochondrial ATP-sensitive potassium channel opener, prolongs survival in HSPB5 R120G transgenic mice. PloS one 26 21541347
2018 The Role of the Arginine in the Conserved N-Terminal Domain RLFDQxFG Motif of Human Small Heat Shock Proteins HspB1, HspB4, HspB5, HspB6, and HspB8. International journal of molecular sciences 25 30036999
2013 Analysis of the dominant effects mediated by wild type or R120G mutant of αB-crystallin (HspB5) towards Hsp27 (HspB1). PloS one 24 23950959
2019 The small heat shock proteins, HSPB1 and HSPB5, interact differently with lipid membranes. Cell stress & chaperones 23 31338686
1990 Human alpha B-crystallin (CRYA2) gene mapped to chromosome 11q12-q23. Human genetics 23 2370055
2013 αB-crystallin/HspB5 regulates endothelial-leukocyte interactions by enhancing NF-κB-induced up-regulation of adhesion molecules ICAM-1, VCAM-1 and E-selectin. Angiogenesis 21 23929007
2023 Salvianolic acid A improve mitochondrial respiration and cardiac function via inhibiting apoptosis pathway through CRYAB in diabetic cardiomyopathy. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 20 36773525
2010 Later retinal degeneration following childhood surgical aphakia in a family with recessive CRYAB mutation (p.R56W). Ophthalmic genetics 20 20141356
2020 Mog1 knockout causes cardiac hypertrophy and heart failure by downregulating tbx5-cryab-hspb2 signalling in zebrafish. Acta physiologica (Oxford, England) 19 33032360
2007 HMGA1 mediates the activation of the CRYAB promoter by BRG1. DNA and cell biology 19 17723105
2016 HspB5/αB-crystallin increases dendritic complexity and protects the dendritic arbor during heat shock in cultured rat hippocampal neurons. Cellular and molecular life sciences : CMLS 18 27085702
2009 Changes in retinal alphaB-crystallin (cryab) RNA transcript levels during periods of altered ocular growth in chickens. Experimental eye research 18 19878675
2020 A novel dominant mutation in CRYAB gene leading to a severe phenotype with childhood onset. Molecular genetics & genomic medicine 16 32420686
2022 In high-grade ovarian carcinoma, platinum-sensitive tumor recurrence and acquired-resistance derive from quiescent residual cancer cells that overexpress CRYAB, CEACAM6, and SOX2. The Journal of pathology 15 35302657
2016 The Human 343delT HSPB5 Chaperone Associated with Early-onset Skeletal Myopathy Causes Defects in Protein Solubility. The Journal of biological chemistry 15 27226619
2022 CRYAB reduces cigarette smoke-induced inflammation, apoptosis, and oxidative stress by retarding PI3K/Akt and NF-κB signaling pathways in human bronchial epithelial cells. Allergologia et immunopathologia 14 36086960
2020 Chaperone-Like Activity of HSPB5: The Effects of Quaternary Structure Dynamics and Crowding. International journal of molecular sciences 14 32668633
2018 Sub-Toxic Human Amylin Fragment Concentrations Promote the Survival and Proliferation of SH-SY5Y Cells via the Release of VEGF and HspB5 from Endothelial RBE4 Cells. International journal of molecular sciences 14 30463298
2018 HSPB5 engages multiple states of a destabilized client to enhance chaperone activity in a stress-dependent manner. The Journal of biological chemistry 14 30567736
2015 Rescue of αB Crystallin (HSPB5) Mutants Associated Protein Aggregation by Co-Expression of HSPB5 Partners. PloS one 14 25961584
2011 Association of Alpha B-Crystallin (CRYAB) genotypes with breast cancer susceptibility in Taiwan. Cancer genomics & proteomics 14 21980040
2022 Novel mycoplasma nucleomodulin MbovP475 decreased cell viability by regulating expression of CRYAB and MCF2L2. Virulence 13 36121023
2020 HspB5 Activates a Neuroprotective Glial Cell Response in Experimental Tauopathy. Frontiers in neuroscience 13 32595446
2013 Differential expression and regulation of Cryab in mouse uterus during preimplantation period. Reproduction (Cambridge, England) 13 23579188
2013 CRYAB modulates the activation of CD4+ T cells from relapsing-remitting multiple sclerosis patients. Multiple sclerosis (Houndmills, Basingstoke, England) 13 23736536
2010 αB-crystallin (HspB5) in familial amyloidotic polyneuropathy. International journal of experimental pathology 13 20804537
2021 Identification of CRYAB+ KCNN3+ SOX9+ Astrocyte-Like and EGFR+ PDGFRA+ OLIG1+ Oligodendrocyte-Like Tumoral Cells in Diffuse IDH1-Mutant Gliomas and Implication of NOTCH1 Signalling in Their Genesis. Cancers 12 33925547
2019 αB-crystallin (CRYAB) regulates the proliferation, apoptosis, synthesis and degradation of extracellular matrix of chondrocytes in osteoarthritis. Experimental cell research 12 31226261
2019 Anti-inflammatory and Oto-Protective Effect of the Small Heat Shock Protein Alpha B-Crystallin (HspB5) in Experimental Pneumococcal Meningitis. Frontiers in neurology 12 31244750
2006 CRYAB promoter polymorphisms: influence on multiple sclerosis susceptibility and clinical presentation. Clinica chimica acta; international journal of clinical chemistry 12 17010329
2024 In-depth analysis of serum antibodies against Epstein-Barr virus lifecycle proteins, and EBNA1, ANO2, GlialCAM and CRYAB peptides in patients with multiple sclerosis. Frontiers in immunology 11 39742283
2022 The crystallin alpha B (HSPB5)-tripartite motif containing 33 (TRIM33) axis mediates myocardial fibrosis induced by angiotensinogen II through transforming growth factor-β (TGF-β1)-Smad3/4 signaling. Bioengineered 11 35333698
2016 Induction and phosphorylation of the small heat shock proteins HspB1/Hsp25 and HspB5/αB-crystallin in the rat retina upon optic nerve injury. Cell stress & chaperones 11 26475352
2021 Analysis of amyloid-like secondary structure in the Cryab-R120G knock-in mouse model of hereditary cataracts by two-dimensional infrared spectroscopy. PloS one 10 34520490
2020 Effect of cataract-associated mutations in the N-terminal domain of αB-crystallin (HspB5). Experimental eye research 10 32533979
2022 Role of methylation-related genes CRYAB and SLC39A11 in the occurrence and development of lung adenocarcinoma. Annals of translational medicine 9 36388803
2021 Long Noncoding RNA lnc-TSSK2-8 Activates Canonical Wnt/β-Catenin Signaling Through Small Heat Shock Proteins HSPA6 and CRYAB. Frontiers in cell and developmental biology 9 34041241
2019 Adipocyte abundances of CES1, CRYAB, ENO1 and GANAB are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regain. Adipocyte 9 31037987
2019 Radiation-Stimulated Translocation of CD166 and CRYAB to the Endothelial Surface Provides Potential Vascular Targets on Irradiated Brain Arteriovenous Malformations. International journal of molecular sciences 9 31757032
2008 HspB5/alphaB-crystallin: properties and current progress in neuropathy. Current neurovascular research 9 18473830
2024 The activation of LBH-CRYAB signaling promotes cardiac protection against I/R injury by inhibiting apoptosis and ferroptosis. iScience 8 38660406
2022 Effects of the CRYAB gene on stem cell-like properties of colorectal cancer and its mechanism. Journal of cancer research and therapeutics 8 36204880
2020 HspB5/αB-crystallin phosphorylation at S45 and S59 is essential for protection of the dendritic tree of rat hippocampal neurons. Journal of neurochemistry 8 33220080
2017 Correlations of single nucleotide polymorphisms of CRYAA and CRYAB genes with the risk and clinicopathological features of children suffering from congenital cataract. Medicine 8 28640093
2013 Characterization of migration parameters on peripheral and central nervous system T cells following treatment of experimental allergic encephalomyelitis with CRYAB. Journal of neuroimmunology 8 23602713
2024 Mutation of CRYAB encoding a conserved mitochondrial chaperone and antiapoptotic protein causes hereditary optic atrophy. JCI insight 7 39561005
2021 PIK3R1, SPNB2, and CRYAB as Potential Biomarkers for Patients with Diabetes and Developing Acute Myocardial Infarction. International journal of endocrinology 7 34887920
2020 Phosphorylation of the Chaperone-Like HspB5 Rescues Trafficking and Function of F508del-CFTR. International journal of molecular sciences 7 32650630
2025 Activation mechanism of small heat shock protein HSPB5 revealed by disease-associated mutants. Proceedings of the National Academy of Sciences of the United States of America 6 40377988
2022 Omega-3 fatty acids decrease CRYAB, production of oncogenic prostaglandin E2 and suppress tumor growth in medulloblastoma. Life sciences 6 35157910
2022 Human HspB1, HspB3, HspB5 and HspB8: Shaping these disease factors during vertebrate evolution. Cell stress & chaperones 6 35678958