Affinage

CENPT

Centromere protein T · UniProt Q96BT3

Length
561 aa
Mass
60.4 kDa
Annotated
2026-04-28
33 papers in source corpus 25 papers cited in narrative 25 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CENP-T is an inner kinetochore histone-fold protein that nucleates a parallel pathway for outer kinetochore assembly at centromeres. Together with CENP-W, CENP-S, and CENP-X, it forms a nucleosome-like heterotetramer that binds centromeric linker DNA and introduces positive supercoils, with DNA contacts in CENP-T and CENP-W essential for this activity and for kinetochore targeting (PMID:22304917, PMID:24234442). The intrinsically disordered N-terminal region of CENP-T functions as a CDK1-phosphorylated scaffold that directly recruits up to two Ndc80 complexes and one Mis12-associated Ndc80 complex, operating in parallel with the CENP-C pathway to collectively assemble two MIS12 and up to four NDC80 complexes per kinetochore unit (PMID:23334297, PMID:28012276, PMID:35165266). Cell-cycle-coupled deposition of CENP-T at centromeres is mediated by the FACT chaperone complex and HJURP, while Aurora B and CDK1 phosphorylation events regulate CENP-T–CENP-W stability and the phospho-dependent switching from interphase binding partners to mitotic Ndc80 engagement (PMID:27284163, PMID:30459232, PMID:34810257, PMID:38200711).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2008 Medium

    Before the molecular function of CENP-T was known, live-cell FRET and FRAP established that CENP-T physically associates with CENP-A chromatin and is deposited at centromeres specifically during S phase, framing it as a replication-coupled centromere component.

    Evidence Acceptor-bleaching FRET and FRAP in live human cells

    PMID:19412974

    Open questions at the time
    • Single-lab FRET study without biochemical confirmation of direct CENP-A contact
    • Mechanism of S-phase coupling unknown
  2. 2012 High

    Structural and biochemical work revealed that CENP-T forms a nucleosome-like CENP-T-W-S-X heterotetramer with histone-fold domains that binds and positively supercoils DNA, establishing the molecular basis for how CENP-T integrates into centromeric chromatin, while yeast reconstitution showed the conserved N-terminal peptide directly recruits the Ndc80 complex via Spc24-Spc25.

    Evidence Crystal structure, in vitro DNA-binding/supercoiling assays, mutagenesis, in vivo kinetochore assembly (human); biochemical reconstitution and mini-chromosome segregation assay (yeast)

    PMID:22304917 PMID:22561346

    Open questions at the time
    • How the heterotetramer is positioned relative to CENP-A nucleosomes in vivo
    • Stoichiometry of DNA wrapping at native centromeres
  3. 2013 High

    Crystal structures of the CENP-T N-terminus bound to Ndc80 RWD domains revealed that CDK phosphorylation strengthens this interaction through a cryptic hydrophobic mechanism, and demonstrated that CENP-T–Ndc80 and Mis12–Ndc80 binding are mutually exclusive, defining two independent Ndc80 recruitment routes.

    Evidence X-ray crystallography, ITC, phospho-mutant analysis, co-immunoprecipitation, in vivo recruitment assays (human); DNA preference and supercoiling domain analysis

    PMID:23334297 PMID:24234442

    Open questions at the time
    • How the two pathways are coordinated temporally in mitosis
    • Whether CDK phosphorylation is switch-like or graded in vivo
  4. 2015 High

    Ectopic targeting experiments separated the CENP-T and CENP-C pathways: Aurora B promotes KMN recruitment to CENP-C while CDK regulates KMN recruitment to CENP-T, establishing distinct kinase-dependent regulatory logic for each parallel pathway.

    Evidence Ectopic chromosomal locus targeting, kinase inhibitor treatment, quantitative fluorescence imaging in human cells; genetic epistasis and Mps1 regulation in budding yeast

    PMID:25660545 PMID:25716979

    Open questions at the time
    • Quantitative contribution of each pathway to total kinetochore-microtubule attachment strength
    • Cross-talk between Aurora B and CDK at CENP-T
  5. 2016 High

    Reconstitution of the full phospho-regulated CENP-T scaffold showed that CDK1-dependent triple phosphorylation enables recruitment of one Mis12:Ndc80 complex and two additional Ndc80 complexes, and together with CENP-C accounts for the measured kinetochore stoichiometry of two MIS12 and up to four NDC80 complexes.

    Evidence In vitro reconstitution with phospho-mutants, electron microscopy, quantitative binding assays

    PMID:28012276

    Open questions at the time
    • Whether this stoichiometry is invariant across cell types
    • How stoichiometry is maintained during metaphase oscillations
  6. 2016 High

    Identification of the FACT chaperone as the loading machine for CENP-T/W answered how CENP-T is deposited at centromeres: FACT binds the histone-fold domain of CENP-T/W and is sufficient for de novo centromeric accumulation, while genomic mapping placed CENP-T over CENP-B boxes between flanking CENP-A nucleosomes.

    Evidence Proteomic screen, reciprocal Co-IP, domain mapping, RNAi depletion, ectopic targeting (human cells); ChIP-seq and sequential ChIP at base-pair resolution

    PMID:27284163 PMID:27384170

    Open questions at the time
    • Whether FACT and HJURP act sequentially or in parallel for CENP-T loading
    • Structural basis of FACT–CENP-T interaction
  7. 2018 Medium

    HJURP was identified as a second chaperone that directly binds the CENP-T C-terminus and is required for S/G2-phase CENP-T recruitment, while yeast de novo assembly assays proved the CENP-T and Mis12 pathways are functionally redundant for Ndc80 recruitment and viability.

    Evidence CRISPR knockout, domain mapping, Co-IP, cell-cycle staged immunofluorescence (human); in vitro kinetochore assembly and genetic epistasis (yeast)

    PMID:30117803 PMID:30459232

    Open questions at the time
    • Whether HJURP–CENP-T interaction occurs on or off chromatin
    • How FACT and HJURP coordinate during the loading cycle
  8. 2020 Medium

    Structural resolution of the yeast CENP-I module (Ctf3c) bound to CENP-T/W revealed the architectural basis for inner kinetochore co-recruitment, while oocyte studies uncovered an unexpected role for CENP-T upstream of APC-CDH1 in meiotic resumption.

    Evidence Crystal structure and live-cell imaging (yeast); siRNA knockdown with overexpression rescue in mouse oocytes

    PMID:31964702 PMID:32679099

    Open questions at the time
    • Whether the CDH1 regulatory role is kinetochore-dependent or represents a moonlighting function
    • Whether Ctf3c–CENP-T/W structural interface is conserved in vertebrates
  9. 2021 Medium

    CDK1 phosphorylation of the CENP-T N-terminal CIM motif was shown to act as a molecular switch that displaces the interphase partner Ccp1 to allow mitotic Ndc80 binding, providing a mechanism for the temporal transition from centromere maintenance to kinetochore assembly.

    Evidence Phospho-mutant analysis, Co-IP, live-cell imaging, chromosome segregation assays in fission yeast

    PMID:34810257

    Open questions at the time
    • Whether an analogous switching mechanism operates in vertebrates
    • Identity of a vertebrate Ccp1 equivalent
  10. 2022 High

    Genetic engineering in vertebrate DT40 cells demonstrated that two Ndc80 complexes on a single CENP-T molecule are functionally required for proper kinetochore–microtubule interactions, and that artificial direct tethering of two Ndc80 copies can bypass the need for Mis12-mediated linkage.

    Evidence Interaction-specific mutations, artificial tethering constructs, chromosome segregation and spindle checkpoint assays in chicken DT40 cells

    PMID:35165266

    Open questions at the time
    • Whether this design principle holds for human kinetochores in vivo
    • How two Ndc80 copies on one CENP-T engage a single microtubule
  11. 2024 High

    Three complementary studies resolved outstanding questions: the CENP-T–Mis12 interface involves three binding surfaces cooperatively regulated by dual phosphorylation; Aurora B phosphorylation of CENP-W T60 stabilizes the CENP-W–CENP-T interaction for accurate segregation; and Ndc80 binding to CENP-T proceeds through a two-step maturation mechanism that is dramatically accelerated by molecular clustering of CENP-T.

    Evidence AlphaFold2 prediction with biochemical validation and phospho-mutants (DT40); kinase assay, Co-IP, and segregation assays; quantitative in vitro kinetics with single-molecule imaging and live-cell validation (human)

    PMID:38200711 PMID:39628583 PMID:39700145

    Open questions at the time
    • In vivo clustering stoichiometry at native centromeres
    • Whether maturation kinetics differ across cell types or species
  12. 2025 Medium

    Adaptive evolution of the CENP-T histone-fold domain in mice reduced centromere binding in a manner that supports female gametogenesis, revealing that CENP-T centromere affinity is under evolutionary selection independent of centromeric DNA sequence.

    Evidence Transgenic mouse models with chimeric CENP-T variants, quantitative centromere binding assays, gametogenesis phenotyping

    PMID:39947176

    Open questions at the time
    • Whether this adaptation impacts kinetochore strength or meiotic drive
    • Molecular basis of the histone-fold changes that alter binding

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the precise structural organization of the CENP-T-W-S-X complex on native centromeric chromatin in situ, the coordination between FACT and HJURP chaperones during CENP-T deposition, and whether the Ndc80 maturation kinetics observed in vitro govern error correction dynamics in vivo.
  • No in situ structural view of CENP-T on centromeric chromatin
  • FACT vs. HJURP temporal coordination unresolved
  • Physiological relevance of Ndc80 binding maturation kinetics for error correction

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 4 GO:0060090 molecular adaptor activity 4 GO:0003677 DNA binding 2
Localization
GO:0005694 chromosome 4 GO:0005634 nucleus 2
Pathway
R-HSA-1640170 Cell Cycle 4 R-HSA-4839726 Chromatin organization 3
Partners
CENPSCENPWCENPXDSN1HJURPSPC24SPC25SUPT16H
Complex memberships
CCAN (constitutive centromere-associated network)CENP-T-W-S-X heterotetramer

Evidence

Reading pass · 25 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2012 CENP-T-W and CENP-S-X complexes coassemble to form a stable CENP-T-W-S-X heterotetramer with histone-fold domains that structurally resembles a nucleosome; the heterotetramer binds DNA and introduces supercoiling, and mutations disrupting heterotetramerization or DNA-protein contacts reduce DNA binding/supercoiling in vitro and compromise kinetochore assembly in vivo. Crystal structure, in vitro DNA binding and supercoiling assays, active-site/interface mutagenesis, in vivo kinetochore assembly assays Cell High 22304917
2013 The N-terminal disordered region of vertebrate CENP-T directly interacts with the RWD domain of Spc24/Spc25 (Ndc80 complex); CDK phosphorylation of CENP-T strengthens a cryptic hydrophobic interaction with Spc25 in a phospho-regulated manner that does not require direct recognition of the phosphorylated residue. The CENP-T–Ndc80 and Mis12–Ndc80 interactions are mutually exclusive, defining two parallel pathways for Ndc80 recruitment. X-ray crystal structure, ITC, phospho-mutant analysis, co-immunoprecipitation, in vivo kinetochore recruitment assays The EMBO journal High 23334297
2012 The histone-fold protein Cnn1 (yeast CENP-T ortholog) is a direct centromere receptor of the Ndc80 complex; its conserved N-terminal peptide motif mediates stoichiometric binding to the Spc24-Spc25 domain; artificial tethering of Ndc80 through Cnn1 supports mini-chromosome segregation without a natural centromere. Biochemical reconstitution, pulldown, in vivo genetic complementation assay, mini-chromosome segregation assay Nature cell biology High 22561346
2013 The CENP-T-W-S-X complex preferentially binds ~100 bp of linker DNA (not nucleosome-bound DNA), primarily as a (CENP-T-W-S-X)₂ dimer of tetramers, and unlike canonical nucleosomes induces positive rather than negative DNA supercoils; DNA-binding regions in CENP-T and CENP-W (but not CENP-S or CENP-X) are required for positive supercoiling and kinetochore targeting. In vitro DNA binding assays, supercoiling assays, domain mutagenesis, in vivo localization assays Nucleic acids research High 24234442
2015 CENP-T and CENP-C act in parallel but distinct pathways to recruit the KMN network: CENP-C recruits Ndc80 via KNL1 and Mis12, whereas CENP-T directly interacts with Ndc80, which in turn recruits KNL1/Mis12. Aurora B kinase promotes KMN recruitment to CENP-C, while CDK regulates KMN recruitment to CENP-T. Ectopic chromosomal locus targeting, co-immunoprecipitation, kinase inhibitor treatment, quantitative fluorescence imaging Current biology : CB High 25660545
2016 CDK1:Cyclin B phosphorylates CENP-T at three distinct N-terminal sites, enabling CENP-T to bind one MIS12:NDC80 complex and two additional NDC80 complexes; CENP-C and CENP-T together can recruit two MIS12 and up to four NDC80 complexes, explaining stoichiometry of kinetochore components. In vitro reconstitution, phospho-mutant analysis, electron microscopy visualization of reconstituted complexes, quantitative binding assays eLife High 28012276
2016 The histone chaperone FACT (subunits Spt16/SSRP1) binds CENP-T/W; the C-terminal domain of Spt16 specifically binds the histone fold region of CENP-T/W. Depletion of Spt16 impairs CENP-T and CENP-W deposition at centromeres, and site-directed targeting of Spt16 alone is sufficient to drive de novo CENP-T accumulation at centromeres. Proteomic screen, co-immunoprecipitation, domain mapping, RNAi depletion, ectopic targeting assay, immunofluorescence Genes & development High 27284163
2008 In living human cells, CENP-T directly associates with CENP-A and CENP-B (detected by FRET); CENP-T exchange at centromeres is restricted to S-phase (shown by FRAP), indicating a co-replicational loading mechanism. Acceptor-bleaching FRET, FRAP in live cells Journal of biophotonics Medium 19412974
2013 CSN5/JAB1 directly interacts with both CENP-T and CENP-W (yeast two-hybrid and co-immunoprecipitation) and promotes ubiquitin- and proteasome-dependent degradation of CENP-T and CENP-W; formation of the CENP-T/W complex enhances protein stability by blocking CSN5-mediated degradation; CSN5 dysregulation impairs CENP-T/W recruitment to kinetochores during prophase. Yeast two-hybrid, co-immunoprecipitation, proteasome inhibitor experiments, in vivo localization assays The Journal of biological chemistry Medium 23926101
2015 In budding yeast, Cnn1 (CENP-T) harbors two kinetochore-localization activities: a C-terminal histone-fold domain associating with the centromere region, and an N-terminal Spc24/Spc25 interaction sequence (residues 25-91) mediating linkage to the Ndc80 complex; Mps1 kinase phosphorylates Cnn1-S74, regulating its interaction with Ndc80 and modulating kinetochore accumulation from G1 through metaphase. In vivo localization by fluorescence microscopy, domain deletion/mutation analysis, kinase in vitro phosphorylation assay, genetic epistasis Genetics Medium 25716979
2018 CENP-T directly binds HJURP (a CENP-A chaperone) via the C-terminus of CENP-T; HJURP knockout minimizes CENP-T recruitment to centromeres; a HJURP-binding-deficient CENP-T mutant fails to localize to centromeres; HJURP recruits CENP-T in S/G2 phase. Co-immunoprecipitation, CRISPR knockout, domain mapping mutagenesis, immunofluorescence cell cycle staging The Journal of biological chemistry Medium 30459232
2015 In fission yeast, the CENP-A (Cnp1) N-tail specifically promotes localization of CENP-T (Cnp20) (but not CENP-C) at centromeres; overexpression of CENP-T suppresses centromere inactivation defects caused by N-tail mutations, placing CENP-T downstream of the CENP-A N-tail in a pathway that maintains epigenetic centromere stability. Genetic epistasis (suppressor overexpression), fluorescence microscopy localization, synthetic lethality analysis Current biology : CB Medium 25619765
2016 By in vivo FRET in human cells, the CENP-T C-terminus is specifically proximal to histone H3.1 (but not H3.2, H3.3, or CENP-A), suggesting CENP-T bridges a CENP-A-containing and an H3.1-containing nucleosome at centromeres. In vivo acceptor-bleaching FRET in live human cells International journal of molecular sciences Medium 25775162
2016 ChIP-seq and sequential ChIP in human cells show that CENPT is centered over the CENPB box between two well-positioned CENPA nucleosomes on α-satellite dimers and physically interacts with the CENPB/CENPC complex; cross-linking captures the entire CENPA/CENPB/CENPC/CENPT complex over an α-satellite dimer. ChIP-seq, sequential ChIP, base-pair resolution genomic readout Genome research Medium 27384170
2018 In budding yeast, de novo kinetochore assembly assay demonstrates that when the Mis12 pathway is crippled (Dsn1 phosphorylation defect), the CENP-T pathway becomes essential for viability and Ndc80 complex recruitment, establishing functional redundancy and epistatic relationship between the two Ndc80 recruitment pathways. De novo kinetochore assembly in yeast extracts, genetic epistasis, microtubule-binding assay eLife Medium 30117803
2020 Crystal structure of the Ctf3 complex (yeast CENP-I module) bound to the Cnn1-Wip1 (CENP-T/W) heterodimer reveals the structural basis for Ctf3c and Cnn1-Wip1 co-recruitment to the kinetochore; live-cell imaging provides a feedback regulation mechanism for Ctf19c assembly. High-resolution crystal structure, live-cell imaging Current biology : CB Medium 32679099
2021 In fission yeast, CDK1-mediated phosphorylation of the Ccp1-interaction motif (CIM) at the N-terminus of CENP-T disrupts Ccp1 binding, enabling competitive displacement of Ccp1 by Ndc80; the phospho-null CIM mutant retains Ccp1 at centromeres during mitosis and mispositions the Ndc80 complex, causing chromosome missegregation. Phospho-mutant analysis, co-immunoprecipitation, live-cell imaging, chromosome segregation assays in fission yeast Proceedings of the National Academy of Sciences of the United States of America Medium 34810257
2022 In chicken DT40 cells, two copies of Ndc80 complex (N-N) on CENP-T (one via direct binding, one via Mis12C) are required for proper kinetochore-microtubule interactions; artificial direct attachment of two Ndc80 complexes to CENP-T can substitute for the native Mis12C-mediated linkage, demonstrating N-N functionality is independent of direct Mis12C-Ndc80 binding. DT40 cell genetic engineering (interaction mutants), artificial kinetochore tethering, chromosome segregation and spindle checkpoint assays Nature communications High 35165266
2024 The CENP-T–Mis12 complex interaction occurs via three binding surfaces (identified by AlphaFold2 combined with biochemical validation); this interaction is cooperatively regulated by dual phosphorylation of Dsn1 (Mis12C component) and CENP-T, ensuring robust Mis12C recruitment to CENP-T during mitosis. AlphaFold2 structure prediction combined with cell biological and biochemical validation, phospho-mutant analysis in DT40 cells iScience Medium 39628583
2024 Aurora B phosphorylates CENP-W at threonine 60, which enhances the CENP-W–CENP-T interaction (via the histone fold domain and an uncharacterized N-terminal region of CENP-T) to ensure robust metaphase chromosome alignment and accurate chromosome segregation. In vitro kinase assay, co-immunoprecipitation, phospho-mutant analysis, live-cell imaging, chromosome segregation assays Journal of molecular cell biology Medium 38200711
2024 Ndc80 binding to CENP-T is a two-step process: rapid initial association/dissociation at disordered N-terminal sites followed by a slower 'maturation' transition to stronger retention; this maturation kinetic barrier is markedly accelerated when CENP-T is clustered at high molecular density, explaining why clustered CENP-T recruits more Ndc80 than monomeric CENP-T, and the two Ndc80-binding sites on CENP-T exhibit distinct maturation rates. Quantitative in vitro binding assays with clustered vs. monomeric CENP-T, single-molecule imaging, live dividing human cell analysis Proceedings of the National Academy of Sciences of the United States of America High 39700145
2020 Depletion of CENP-T by siRNA in mouse oocytes increases CDH1/FZR1 levels, elevating APC-CDH1 activity and decreasing CCNB1, thereby attenuating MPF and severely compromising meiotic resumption (G2/M transition); these defects are rescued by CCNB1 overexpression or CDH1 knockdown, placing CENP-T upstream of CDH1 in regulating meiotic progression. siRNA knockdown, overexpression rescue, western blot for CDH1 and CCNB1, MPF activity assay, genetic epistasis in mouse oocytes Journal of cell science Medium 31964702
2016 In Xenopus egg extracts, CENP-T centromeric recruitment occurs in late interphase independently of DNA synthesis and precedes CENP-W recruitment (which occurs in mitosis); unlike CENP-C, CENP-T does not participate in CENP-A deposition; depletion of CENP-C reduces CENP-T at centromeres, but kinetochores can still assemble with reduced Ndc80/Mis12, supporting the existence of two parallel assembly pathways. Xenopus egg cell-free extract immunodepletion, cell cycle staging, immunofluorescence, western blot Nucleus (Austin, Tex.) Medium 25569378
2025 CENPT interacts with GCLC (γ-glutamyl-cysteine ligase catalytic subunit) by binding to residues 213-424 of GCLC competitively with GCLM (the modifier subunit), increasing GCLC catalytic activity and glutathione synthesis, thereby reducing ROS and inhibiting ferroptosis in renal cell carcinoma cells. Co-immunoprecipitation, domain mapping, ROS measurements, ferroptosis assays, GSH quantification Cell death & disease Low 40651948
2025 Adaptive evolution of the CENP-T histone fold domain (DNA-binding region) in mice reduced centromere binding; introducing the histone fold domain from closely related species into mouse CENP-T (chimeric variants) increased centromere binding in oocytes and somatic cells; reduced binding by mouse CENP-T supports robust female gametogenesis, and the adaptation is independent of centromeric DNA sequence. Transgenic mouse models with chimeric CENP-T variants, oocyte microinjection, quantitative centromere binding assays, gametogenesis phenotype analysis Current biology : CB Medium 39947176

Source papers

Stage 0 corpus · 33 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2012 CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold. Cell 206 22304917
2013 CENP-T provides a structural platform for outer kinetochore assembly. The EMBO journal 169 23334297
2012 CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nature cell biology 151 22561346
2015 Distinct organization and regulation of the outer kinetochore KMN network downstream of CENP-C and CENP-T. Current biology : CB 115 25660545
2016 Molecular basis of outer kinetochore assembly on CENP-T. eLife 106 28012276
2017 Integrity of the human centromere DNA repeats is protected by CENP-A, CENP-C, and CENP-T. Proceedings of the National Academy of Sciences of the United States of America 96 28167779
2013 The centromeric nucleosome-like CENP-T-W-S-X complex induces positive supercoils into DNA. Nucleic acids research 65 24234442
2020 CenH3-Independent Kinetochore Assembly in Lepidoptera Requires CCAN, Including CENP-T. Current biology : CB 52 32032508
2018 An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast. eLife 51 30117803
2016 The CENP-T/-W complex is a binding partner of the histone chaperone FACT. Genes & development 46 27284163
2015 The CENP-A N-tail confers epigenetic stability to centromeres via the CENP-T branch of the CCAN in fission yeast. Current biology : CB 38 25619765
2016 CENPT bridges adjacent CENPA nucleosomes on young human α-satellite dimers. Genome research 37 27384170
2008 Live-cell imaging reveals sustained centromere binding of CENP-T via CENP-A and CENP-B. Journal of biophotonics 26 19412974
2022 Recruitment of two Ndc80 complexes via the CENP-T pathway is sufficient for kinetochore functions. Nature communications 25 35165266
2016 Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability. Open biology 20 26791246
2015 The distinct functions of CENP-C and CENP-T/W in centromere propagation and function in Xenopus egg extracts. Nucleus (Austin, Tex.) 19 25569378
2020 The Structural Basis for Kinetochore Stabilization by Cnn1/CENP-T. Current biology : CB 16 32679099
2013 CSN5/JAB1 interacts with the centromeric components CENP-T and CENP-W and regulates their proteasome-mediated degradation. The Journal of biological chemistry 15 23926101
2015 The Mps1 kinase modulates the recruitment and activity of Cnn1(CENP-T) at Saccharomyces cerevisiae kinetochores. Genetics 14 25716979
2018 Holliday junction recognition protein interacts with and specifies the centromeric assembly of CENP-T. The Journal of biological chemistry 10 30459232
2017 A defect in the inner kinetochore protein CENPT causes a new syndrome of severe growth failure. PloS one 10 29228025
2020 CENP-T regulates both the G2/M transition and anaphase entry by acting through CDH1 in meiotic oocytes. Journal of cell science 7 31964702
2025 CENPT prevents renal cell carcinoma against ferroptosis by enhancing the synthesis of glutathione. Cell death & disease 6 40651948
2025 Adaptive evolution of CENP-T modulates centromere binding. Current biology : CB 5 39947176
2021 Ccp1-Ndc80 switch at the N terminus of CENP-T regulates kinetochore assembly. Proceedings of the National Academy of Sciences of the United States of America 4 34810257
2015 The CENP-T C-terminus is exclusively proximal to H3.1 and not to H3.2 or H3.3. International journal of molecular sciences 4 25775162
2024 Binding site maturation modulated by molecular density underlies Ndc80 binding to kinetochore receptor CENP-T. Proceedings of the National Academy of Sciences of the United States of America 2 39700145
2024 Aurora B promotes the CENP-T-CENP-W interaction to guide accurate chromosome segregation in mitosis. Journal of molecular cell biology 1 38200711
2024 Binding Site Maturation Modulated by Molecular Density Underlies Ndc80 Binding to Kinetochore Receptor CENP-T. bioRxiv : the preprint server for biology 1 38464265
2024 Molecular details and phosphoregulation of the CENP-T-Mis12 complex interaction during mitosis in DT40 cells. iScience 1 39628583
2025 Insects evolved a monomeric histone-fold domain in the CENP-T protein family. EMBO reports 0 41162737
2025 Fused in Sarcoma (FUS) promotes renal cell carcinoma progression via the KCMF1/FUS/CENPT axis and activation of the JNK signaling pathway. Journal of translational medicine 0 41184988
2023 Bioinformatics insights into CENP-T and CENP-W protein-protein interaction disruptive amino acid substitution in the CENP-T-W complex. Journal of cellular biochemistry 0 37943107