Affinage

CD53

Leukocyte surface antigen CD53 · UniProt P19397

Length
219 aa
Mass
24.3 kDa
Annotated
2026-06-09
57 papers in source corpus 28 papers cited in narrative 27 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CD53 is a leukocyte-restricted tetraspanin that organizes multi-protein membrane complexes to set signaling thresholds across immune cell development, activation, and trafficking (PMID:2258620, PMID:1700763, PMID:8757325). As a scaffold, it laterally associates with integrin α4β1 (PMID:8757325), MHC class II and other tetraspanins within supramolecular surface clusters (PMID:8119731, PMID:8816400), and stabilizes the membrane residence of key signaling receptors: it sustains IL-7Rα surface expression to drive PI3K and JAK/STAT signaling required for the pro-B to pre-B transition (PMID:31748347), stabilizes CD45 (controlling the CD45RO isoform and membrane mobility) to enable optimal CD45 phosphatase activity and downstream Lck activation in T cells (PMID:35767951), and partners with CXCR4 to support CXCL12-driven signaling, receptor internalization, and bone marrow homing (PMID:38363205). CD53 also stabilizes L-selectin by restraining ADAM17-dependent shedding, and loss of CD53 impairs lymphocyte homing and neutrophil transendothelial migration through altered α3 integrin expression and cytoskeletal remodeling (PMID:32428859, PMID:32532837). Antibody ligation of CD53 transduces active signals—mobilizing calcium, translocating PKC, and inducing iNOS-dependent nitric oxide production (PMID:8335905, PMID:7511680)—and engages JNK, ERK, and AKT cascades that variously drive transcription, proliferation, and survival (PMID:11846804, PMID:12606948, PMID:12631118). Beyond classical leukocyte roles, CD53 promotes DREAM-complex-mediated hematopoietic stem cell quiescence by facilitating the Rbl2/p130–PP2A interaction (PMID:36542833) and integrates inflammatory and metabolic signals in hepatocytes to regulate lipid accumulation and cytokine production (PMID:36581203). Its scaffolding is conformation- and glycosylation-dependent, with N-glycosylation limiting partner engagement and protein conformation governing nanoscale clustering (PMID:38031400).

Mechanistic history

Synthesis pass · year-by-year structured walk · 17 steps
  1. 1990 High

    Established CD53 as a four-transmembrane integral membrane glycoprotein of the tetraspanin family, defining the structural class from which all subsequent function was interpreted.

    Evidence cDNA cloning and sequence analysis with transfection-based immunostaining/immunoprecipitation in NIH3T3/COS cells

    PMID:1700763 PMID:2258620

    Open questions at the time
    • Structure inferred from sequence, no experimental 3D structure
    • No function assigned at this stage
  2. 1994 Medium

    Defined CD53 as a signaling-competent receptor by showing that ligation triggers calcium flux, inositol phosphate/DAG generation, PKC translocation, and iNOS-dependent nitric oxide production, independent of GTP-binding proteins.

    Evidence Antibody cross-linking with pharmacological dissection (staurosporine, pertussis/cholera toxins) and biochemical readouts in human and rat myeloid/lymphoid cells

    PMID:7511680 PMID:8335905

    Open questions at the time
    • Antibody cross-linking is non-physiological; native ligand unknown
    • Proximal kinase linking CD53 to these effectors not identified
  3. 1994 Medium

    Showed CD53 physically assembles into large supramolecular membrane complexes with other tetraspanins and MHC class II, establishing its role as a membrane organizer rather than a solitary receptor.

    Evidence Co-immunoprecipitation with preclearing from human B-cell lysates

    PMID:8119731

    Open questions at the time
    • Detergent-based co-IP cannot distinguish direct from indirect association
    • Stoichiometry and architecture of the complex undefined
  4. 1996 High

    Demonstrated specific, reciprocal association of CD53 with integrin α4β1 and nanoscale proximity to MHC molecules, linking the tetraspanin scaffold to adhesion machinery.

    Evidence Reciprocal co-IP across multiple cell lines with adhesion-deficient integrin mutants, plus flow cytometric FRET on B cells

    PMID:8757325 PMID:8816400

    Open questions at the time
    • Functional consequence of the CD53–α4β1 association not tested in this work
    • Direct versus tetraspanin-web-mediated contact unresolved
  5. 1995 Medium

    Linked CD53 to phosphatase-dependent signaling by showing its immunoprecipitates carry tyrosine phosphatase activity acting on Lck, distinct from CD45, foreshadowing later CD45/Lck regulation.

    Evidence Co-IP plus in vitro phosphatase assay on Lck with depletion controls and phosphatase inhibitor

    PMID:7621882

    Open questions at the time
    • Identity of the associated phosphatase not established
    • Functional relevance in intact cells not shown
  6. 2002 Medium

    Connected CD53 ligation to defined intracellular kinase cascades — JNK/Jun transcription, ERK-driven mitogenesis, and AKT-mediated survival — broadening its signaling output beyond calcium/PKC.

    Evidence Kinase activity assays, transcriptional reporters, and pharmacological dissection across lymphoma, mesangial, and renal carcinoma cells

    PMID:11846804 PMID:12606948 PMID:12631118

    Open questions at the time
    • Cascades triggered by antibody ligation, not a physiological ligand
    • Cell-type specificity of which cascade dominates unexplained
  7. 1998 Medium

    Showed CD53 surface levels are dynamically regulated by proteolytic shedding upon neutrophil activation, indicating post-translational control of tetraspanin availability.

    Evidence Flow cytometry, immunoblot, and Northern blot with PMSF protease inhibitor rescue in human neutrophils

    PMID:9620662

    Open questions at the time
    • Responsible protease not identified
    • Functional significance of shedding not tested
  8. 2007 High

    Placed CD53 within HIV-1 assembly compartments, defining these as internalized plasma-membrane tetraspanin domains rather than endosomes.

    Evidence Immunofluorescence, immunoelectron microscopy, and tracer-accessibility assays in HIV-infected macrophages

    PMID:17438075

    Open questions at the time
    • Whether CD53 contributes functionally to viral assembly versus being a passive marker not resolved
  9. 2007 Medium

    Identified EBF1 as a direct transcriptional driver of CD53, placing the gene in the B-cell developmental transcriptional program.

    Evidence EBF1 overexpression with microarray and promoter reporter mapping in BaF/3 cells

    PMID:17429843

    Open questions at the time
    • Other regulators of CD53 expression not mapped
  10. 2019 High

    Established the first in vivo developmental requirement: CD53 sustains IL-7Rα surface expression and PI3K/JAK-STAT signaling needed for B-cell maturation, with direct physical interaction with IL-7R.

    Evidence Cd53-/- mice, bone marrow chimeras, phospho-signaling assays, co-IP and proximity ligation assay

    PMID:31748347

    Open questions at the time
    • Mechanism by which CD53 stabilizes IL-7Rα at the membrane not resolved
    • Direct versus tetraspanin-web interaction undefined
  11. 2020 High

    Defined CD53 as a regulator of leukocyte trafficking by stabilizing L-selectin against ADAM17-dependent shedding and controlling α3 integrin and cytoskeletal remodeling required for homing and transmigration.

    Evidence Cd53-/- mice with flow cytometry, ADAM17 inhibition, intravital microscopy, homing and arthritis models

    PMID:32428859 PMID:32532837

    Open questions at the time
    • How CD53 mechanistically restrains ADAM17 access to L-selectin not detailed
    • ADAM17-independent shedding pathway uncharacterized
  12. 2022 High

    Identified CD45 as an unbiased CD53 interactor and showed CD53 stabilizes CD45, controls the CD45RO isoform, and is required for CD45 phosphatase activity driving Lck activation and T-cell function.

    Evidence Mass spectrometry interactome, Cd53-/- T cells, super-resolution microscopy, phosphatase and Lck assays, tumor rejection model

    PMID:35767951

    Open questions at the time
    • Structural basis of CD53–CD45 interaction unknown
    • Reconciliation with the earlier CD45-independent phosphatase association not addressed
  13. 2022 Medium

    Extended CD53 function beyond leukocytes, showing it integrates metabolic and inflammatory signals in hepatocytes to drive diet-induced dyslipidemia, hepatic inflammation, and lipid accumulation.

    Evidence Cd53-/- mice on Western/NASH diet plus primary hepatocyte siRNA/KO with metabolic and cytokine readouts

    PMID:36581203

    Open questions at the time
    • Molecular partner mediating hepatocyte CD53 signaling not identified
    • Direct versus systemic-immune contribution to phenotype not fully separated
  14. 2023 High

    Revealed a nuclear/intracellular mechanism: CD53 promotes HSC quiescence by facilitating Rbl2/p130–PP2A interaction to stabilize p130 for DREAM complex assembly.

    Evidence Cd53-/- mice, BioID proximity labeling, confocal microscopy, DREAM co-IP, and HSC functional assays under inflammatory stress

    PMID:36542833

    Open questions at the time
    • How a plasma-membrane tetraspanin influences a nuclear repressor complex mechanistically unresolved
    • Whether membrane signaling is upstream of the DREAM effect unknown
  15. 2024 High

    Added CXCR4 to the CD53 interactome, showing CD53 supports CXCL12-driven CXCR4 signaling, internalization, and B-cell bone marrow homing.

    Evidence Proximity ligation assay, migration and internalization assays, and in vivo homing in Cd53-/- mice

    PMID:38363205

    Open questions at the time
    • Whether CD53 directly contacts CXCR4 or acts through the tetraspanin web not resolved
  16. 2023 Medium

    Defined the biophysical rules of CD53 partnering, showing N-glycosylation inhibits engagement of CD45, CD20, and CD37 and protein conformation governs nanoscale clustering.

    Evidence Glycosylation and conformational mutants with co-IP and dSTORM super-resolution microscopy

    PMID:38031400

    Open questions at the time
    • Physiological regulation of CD53 glycosylation/conformation in vivo not established
  17. 2025 Medium

    Implicated CD53 in neutrophil effector function by showing it promotes PI3K/AKT-dependent NET formation in inflammatory disease.

    Evidence Neutralizing antibody, in vitro PMA NET assay with pathway inhibitors, and caerulein-induced pancreatitis mouse model

    PMID:40098997

    Open questions at the time
    • Upstream receptor/ligand engaging CD53 in NETosis unknown
    • Antibody blockade may report on more than physiological CD53 function

Open questions

Synthesis pass · forward-looking unresolved questions
  • The physiological ligand(s) of CD53 and the structural basis for its selective, conformation-dependent recruitment of partners (CD45, IL-7R, CXCR4, integrins) across diverse cell types remain undefined.
  • No endogenous CD53 ligand identified
  • No experimental structure of CD53 or its partner complexes
  • Unifying mechanism linking membrane scaffolding to the nuclear DREAM-complex role unresolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 5 GO:0098772 molecular function regulator activity 3 GO:0005198 structural molecule activity 2
Localization
GO:0005886 plasma membrane 5
Pathway
R-HSA-162582 Signal Transduction 6 R-HSA-168256 Immune System 4 R-HSA-1266738 Developmental Biology 2
Partners
CD37CD81CXCR4IL7RITGA4MHC CLASS II (HLA-DR)PTPRC (CD45)RBL2
Complex memberships
DREAM complex (regulatory association)tetraspanin-enriched microdomain (tetraspanin web)

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1990 CD53 is a 219-amino acid integral membrane protein with four putative transmembrane domains and three N-glycosylation sites, structurally related to CD9, CD37, CD63, and TAPA-1 (tetraspan family). Protein sequence was deduced from cDNA cloning and confirmed by immunostaining/immunoprecipitation of transfected cells. cDNA cloning, sequence analysis, immunostaining and immunoprecipitation of transfected NIH3T3/COS cells Journal of immunology / Immunogenetics High 1700763 2258620
1993 Cross-linking of CD53 on human B cells, monocytes, and granulocytes induces cytoplasmic calcium fluxes; in monocytes it also activates the oxidative burst. The signaling is largely protein kinase C-independent (not blocked by low staurosporine) but sensitive to high staurosporine and not blocked by ADP-ribosylating toxins, suggesting dependence on tyrosine kinases rather than GTP-binding proteins. Cross-linking with F(ab')2 anti-CD53 mAb + secondary antibody; calcium flux measurement; oxidative burst assay; pharmacological inhibitors (staurosporine, pertussis/cholera toxins) Journal of immunology Medium 8335905
1994 CD53 activation by cross-linking in rat macrophages increases inositol phosphates, diacylglycerol, and Ca2+ mobilization (insensitive to pertussis/cholera toxins), causes PKC translocation to the membrane, and induces nitric oxide release via expression of inducible nitric oxide synthase (iNOS) in a PKC- and protein synthesis-dependent manner. Cross-linking of CD53 with mAb; measurement of inositol phosphates, DAG, Ca2+; PKC translocation assay; NO production assay; iNOS protein synthesis inhibition The Journal of experimental medicine Medium 7511680
1994 CD53, CD37, TAPA-1, and R2/C33 tetraspanins co-precipitate with MHC class II DR antigens from B-cell lysates, forming large multicomponent complexes also containing CD19 and CD21. Shown by coprecipitation and preclearing experiments. Co-immunoprecipitation from mild detergent lysates of human B-cell lines and tonsillar B cells Immunogenetics Medium 8119731
1994 Cross-linking of CD53 on resting human B cells promotes G1 cell cycle entry (increased CD69, RNA synthesis, c-myc, cell volume) and, in cooperation with IL-2 or IL-4, induces DNA synthesis and Ig production, demonstrating a functional role in B cell activation. Anti-CD53 mAb cross-linking; flow cytometry for CD69; RNA synthesis measurement; c-myc Western blot; DNA synthesis (7-AAD binding); Ig ELISA Journal of immunology Medium 7963560
1995 Immunoprecipitates of rat CD53 from lymph node and thymoma cell lysates contain tyrosine phosphatase activity capable of dephosphorylating the tyrosine kinase Lck and a synthetic phosphotyrosine substrate in vitro; activity is abrogated by a tyrosine phosphatase inhibitor. The associated phosphatase is not CD45 (shown by depletion experiments). Co-immunoprecipitation; in vitro phosphatase activity assay on Lck and synthetic substrate; depletion experiments; phosphatase inhibitor treatment European journal of immunology Medium 7621882
1996 CD53, CD63, and CD82 (like CD81/TAPA-1) specifically co-precipitate with integrin α4β1 (CD49d/CD29) from hemopoietic cell lines; the association does not require the α4 cytoplasmic domain or divalent cations, but two α4 adhesion-deficient mutants (D346E, D408E) lack this association. Reciprocal co-immunoprecipitation from multiple α4β1-positive cell lines; confocal microscopy for colocalization; mutant integrin analysis Journal of immunology High 8757325
1996 CD53, CD81, and CD82 are in close proximity (within 2–10 nm) with MHC class II (DR, DQ), MHC class I, and CD20 at the B cell surface, forming a single large supramolecular complex as determined by FRET analysis. Flow cytometric FRET (fluorescence resonance energy transfer) with labeled mAbs on JY B cells Journal of immunology Medium 8816400
1997 CD53 ligation induces homotypic adhesion in rat B-cell lymphoma that requires de novo protein synthesis (blocked by cycloheximide/actinomycin D), divalent cations (Ca2+/Mg2+), and is dependent on tyrosine kinases (genistein/piceatannol-sensitive), PI3K (wortmannin-sensitive), and PKC (H7/bisindolylmaleimide-sensitive), but not LFA-1. Anti-CD53 mAb (MRC OX-44) stimulation; pharmacological inhibitors; electron microscopy of cell contact zones Cellular immunology Medium 9225004
1998 Physiological activators of neutrophils (TNFα, PAF, fMLP, PMA, ionomycin) cause down-regulation of CD53 from the neutrophil surface without changing CD53 mRNA levels; this down-regulation is blocked by the serine protease inhibitor PMSF, indicating proteolytic shedding. Flow cytometry; immunoblotting; Northern blot; PMSF protease inhibitor treatment of human neutrophils Journal of leukocyte biology Medium 9620662
2002 CD53 ligation (rat or human) induces a 3–4-fold transient activation of JNK kinase activity (peak 3–5 min) in B-cell and T-cell lymphoma lines, and in renal carcinoma cells transiently transfected with human CD53 cDNA. JNK activation is not mediated by Vav, and CD53 does not cooperate with CD3 for Vav activation. In stable CD53-transfected cells, JNK activation stimulates Jun-dependent transcriptional activity. JNK kinase activity assay on endogenous and transfected cells; transcriptional reporter assay; pharmacological and genetic controls European journal of biochemistry Medium 11846804
2003 CD53 ligation on tumor lymphoma cells triggers a survival signal reducing apoptosis by activating AKT (phospho-Ser473), increasing Bcl-xL levels, decreasing Bax levels (changing Bcl-xL/Bax ratio ~24-fold toward survival), and reducing caspase activation and DNA fragmentation. Anti-CD53 mAb ligation; Western blot for phospho-AKT, Bcl-xL, Bax; PARP cleavage assay; DNA fragmentation assay; serum deprivation model Oncogene Medium 12606948
2003 CD53 ligation on primary rat mesangial cells induces DNA synthesis via ERK1/ERK2 activation; this effect is blocked by the MEK inhibitor PD98059 but not by PI3K, PKC inhibitors, or calcium channel blockers, defining a specific ERK-dependent mitogenic pathway. Thymidine incorporation; ERK phosphorylation by Western blot; pharmacological inhibitors (PD98059, wortmannin, H7, thapsigargin, verapamil); flow cytometry Kidney international Medium 12631118
2004 Stable overexpression of CD53 in macrophages increases intracellular glutathione (GSH) and decreases peroxide levels, conferring resistance to H2O2 and UVB irradiation; antisense CD53 has the opposite effect. CD53 is also induced by LPS and nitric oxide (SNAP) in RAW264.7 macrophages. Stable transfection (sense and antisense CD53); GSH measurement; peroxide assay; H2O2 and UVB survival assays; microarray + Northern blot for induction Molecules and cells Medium 15055538
2007 In macrophages, HIV-1 assembles into an intracellular plasma membrane domain that contains tetraspanins CD81, CD9, and CD53 (but not endosome marker CD63). These CD53/CD81/CD9-positive compartments are connected to the cell surface (accessible to HRP/ruthenium red at 4°C), indicating they are an internalized plasma membrane domain, not endosomes. CD63 is secondarily recruited to the virus-containing compartment. Immunofluorescence microscopy; immunoelectron microscopy; horseradish peroxidase/ruthenium red tracer accessibility assays; tetraspanin co-localization in HIV-infected macrophages The Journal of cell biology High 17438075
2007 CD53 is a direct transcriptional target of the early B cell factor EBF1; functional EBF1 binding sites were identified in the CD53 promoter and confirmed to respond to EBF1 expression in transient transfection assays. Retroviral EBF1 overexpression in BaF/3 cells + microarray; promoter mapping; transient transfection reporter assay European journal of immunology Medium 17429843
2013 siRNA knockdown of CD53 in THP-1 monocytic cells stimulated with house dust mite antigen leads to over-activation of inflammatory cytokine production and increased NF-κB activity, demonstrating that CD53 acts as a suppressor of inflammatory cytokine signaling. siRNA knockdown; cytokine ELISA; NF-κB activity assay; promoter polymorphism functional analysis (EMSA) Biochimica et biophysica acta Medium 23313165
2014 CD53 ligation on rat NK cells reduces degranulation and IFN-γ response to activating receptors (Ly49s3, NKR-P1A, NKp46), induces LFA-1 activation and homotypic NK cell adhesion, and enhances NK cell proliferation in response to IL-2, indicating CD53 modulates NK cell effector functions by promoting adhesion over cytotoxicity. Anti-CD53 mAb ligation; degranulation assay (CD107a); redirected killing assay; IFN-γ ELISA; LFA-1 activation assay (conformational mAb); proliferation assay PloS one Medium 24832104
2019 CD53 promotes IL-7 receptor (IL-7Rα) surface expression and downstream PI3K and JAK/STAT signaling in prepro- and pro-B cells; CD53-deficient mice have reduced IL-7Rα surface expression, impaired IL-7 signaling, increased apoptosis in developing B cells, and a block at the pro-B to pre-B cell transition. CD53 physically interacts with IL-7R as shown by co-immunoprecipitation and proximity ligation assay. Cd53-/- mouse model; mixed bone marrow chimeras; flow cytometry; phospho-signaling (PI3K, JAK/STAT) assays; co-immunoprecipitation; proximity ligation assay (PLA) Journal of immunology High 31748347
2020 CD53 stabilizes L-selectin surface expression on lymphocytes and restrains its shedding via ADAM17-dependent and ADAM17-independent mechanisms. Loss of CD53 in mice results in near absence of L-selectin from B cells and reduced L-selectin stability on T cells, causing impaired lymphocyte homing to lymph nodes and defective antigen-dependent immune responses. Cd53-/- mouse model; flow cytometry for L-selectin; ADAM17 inhibitor experiments; adoptive transfer homing assays; intravital microscopy; human lymphocyte analysis iScience High 32428859
2020 CD53 deficiency in mice impairs neutrophil transendothelial migration induced by TNF, CXCL1, and CCL2, reduces leukocyte retention under shear flow, and causes defects in activation-induced cytoskeletal remodeling. CD53-deficient neutrophils show increased α3 integrin expression and near-complete loss of L-selectin, and demonstrate delayed onset of serum-induced arthritis. Cd53-/- mouse model; intravital microscopy; peritoneal recruitment assays; adhesion molecule flow cytometry; cytoskeletal remodeling imaging; serum-induced arthritis model Journal of immunology High 32532837
2022 CD53 is identified by unbiased mass spectrometry as a partner of CD45. CD53 controls CD45RO isoform expression and membrane mobility, stabilizes total CD45 on the membrane, and is required for optimal CD45 phosphatase activity and subsequent Lck activation. CD53-deficient T cells show substantial proliferation defects and impaired tumor rejection. Cd53-/- mouse model; unbiased mass spectrometry interactome; super-resolution microscopy (membrane mobility); phosphatase activity assay; Lck phosphorylation (Western blot); tumor rejection assay; IFN-γ intracellular staining Cell reports High 35767951
2022 CD53 in hepatocytes integrates inflammatory and nutritional signals: high-fat/fructose exposure and inflammatory stimuli induce CD53 expression in liver; CD53 deletion in mice blocks Western diet-induced dyslipidemia, hepatic inflammatory transcriptome activation, adipose inflammation, and liver lipid accumulation. In isolated hepatocytes, CD53 deletion attenuates TNFα- and fatty acid+LPS-induced cytokine expression and triglyceride accumulation. Cd53-/- mouse model on Western/NASH diet; primary hepatocyte cultures with siRNA/KO; triglyceride assays; cytokine gene expression; transcriptomic analysis; glucose transporter 8 KO epistasis The Journal of biological chemistry Medium 36581203
2023 CD53 promotes DREAM transcriptional repressor complex activity in hematopoietic stem cells (HSCs) by facilitating the interaction between Rbl2/p130 and its phosphatase PP2A, stabilizing p130 for DREAM binding and thereby promoting quiescence. Loss of CD53 causes prolonged cycling and reduced HSC function under inflammatory stress. Cd53-/- mouse model; proximity labeling (BioID); confocal fluorescence microscopy; DREAM complex co-immunoprecipitation; cell cycle analysis; HSC functional assays under inflammatory stress Blood High 36542833
2023 Glycosylation of CD53 inhibits its interaction with partner proteins CD45, CD20, and CD37 (but not vice versa for CD37-CD53); surface expression of CD53 is unaffected by glycosylation. Conformational mutations show that the nanoscale clustering of CD53 depends on its conformation (closed mutant F44E shows higher clustering fraction). N-glycosylation mutants; surface expression flow cytometry; co-immunoprecipitation; dSTORM super-resolution microscopy Biophysical journal Medium 38031400
2024 CD53 physically interacts with CXCR4 (the CXCL12 receptor) on B cells as shown by proximity ligation assay. CD53-deficient B cells show reduced CXCL12-induced CXCR4 signaling and receptor internalization, and impaired bone marrow homing in vivo. Proximity ligation assay; in vitro migration assay toward CXCL12; CXCR4 internalization assay; in vivo bone marrow homing assay in Cd53-/- mice Journal of immunology High 38363205
2025 CD53 promotes neutrophil extracellular trap (NET) formation through the PI3K/AKT pathway; CD53 neutralizing antibody inhibits PMA-induced NETs in vitro and reduces inflammatory injury and NET formation in an acute pancreatitis mouse model. Anti-CD53 neutralizing antibody; in vitro NET model (PMA stimulation); PI3K/AKT pathway inhibitors; caerulein-induced AP mouse model; neutrophil isolation from AP patients Journal of inflammation research Medium 40098997

Source papers

Stage 0 corpus · 57 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2007 In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53. The Journal of cell biology 268 17438075
1996 Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29). Journal of immunology (Baltimore, Md. : 1950) 200 8757325
1996 Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. Journal of immunology (Baltimore, Md. : 1950) 177 8816400
1994 Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/C33) with MHC class II glycoproteins. Immunogenetics 166 8119731
1997 Functional analysis of four tetraspans, CD9, CD53, CD81, and CD82, suggests a common role in costimulation, cell adhesion, and migration: only CD9 upregulates HB-EGF activity. Cellular immunology 128 9514697
1992 C33 antigen recognized by monoclonal antibodies inhibitory to human T cell leukemia virus type 1-induced syncytium formation is a member of a new family of transmembrane proteins including CD9, CD37, CD53, and CD63. Journal of immunology (Baltimore, Md. : 1950) 115 1401919
1990 The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens. Immunogenetics 75 1700763
1990 Identification and analysis of cDNA clones encoding CD53. A pan-leukocyte antigen related to membrane transport proteins. Journal of immunology (Baltimore, Md. : 1950) 69 2258620
1993 CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. Journal of immunology (Baltimore, Md. : 1950) 68 8335905
2006 The effects of intensive, moderate and downhill treadmill running on human blood lymphocytes expressing the adhesion/activation molecules CD54 (ICAM-1), CD18 (beta2 integrin) and CD53. European journal of applied physiology 67 16506060
2020 Tetraspanin CD53: an overlooked regulator of immune cell function. Medical microbiology and immunology 53 32440787
1994 Induction of nitric oxide release by MRC OX-44 (anti-CD53) through a protein kinase C-dependent pathway in rat macrophages. The Journal of experimental medicine 52 7511680
2003 Apoptosis protection and survival signal by the CD53 tetraspanin antigen. Oncogene 49 12606948
1997 Recurrent infectious diseases in human CD53 deficiency. Clinical and diagnostic laboratory immunology 49 9067662
2017 Pathogen lineage-based genome-wide association study identified CD53 as susceptible locus in tuberculosis. Journal of human genetics 45 28878339
2013 Characterization of tetraspanins CD9, CD53, CD63, and CD81 in monocytes and macrophages in HIV-1 infection. Journal of leukocyte biology 44 23570947
1995 Association of the transmembrane 4 superfamily molecule CD53 with a tyrosine phosphatase activity. European journal of immunology 44 7621882
1997 Expression of tetra-spans transmembrane family (CD9, CD37, CD53, CD63, CD81 and CD82) in normal and neoplastic human keratinocytes: an association of CD9 with alpha 3 beta 1 integrin. The British journal of dermatology 39 9470900
1993 Gene structure, chromosomal localization, and protein sequence of mouse CD53 (Cd53): evidence that the transmembrane 4 superfamily arose by gene duplication. International immunology 39 8452817
1993 Epitope mapping of anti-rat CD53 monoclonal antibodies. Implications for the membrane orientation of the Transmembrane 4 Superfamily. European journal of immunology 38 7678222
2000 Increased expression of the tetraspanins CD53 and CD63 on apoptotic human neutrophils. Journal of leukocyte biology 35 10733097
2020 Tetraspanin CD53 Promotes Lymphocyte Recirculation by Stabilizing L-Selectin Surface Expression. iScience 33 32428859
2014 The tetraspanin CD53 modulates responses from activating NK cell receptors, promoting LFA-1 activation and dampening NK cell effector functions. PloS one 31 24832104
1994 Cross-linking of CD53 promotes activation of resting human B lymphocytes. Journal of immunology (Baltimore, Md. : 1950) 31 7963560
1997 Ligation of CD53/OX44, a tetraspan antigen, induces homotypic adhesion mediated by specific cell-cell interactions. Cellular immunology 27 9225004
1997 Anti-CD53 monoclonal antibody induced LFA-1/ICAM-1-dependent and -independent lymphocyte homotypic cell aggregation. Immunobiology 27 9241532
2019 The Tetraspanin CD53 Regulates Early B Cell Development by Promoting IL-7R Signaling. Journal of immunology (Baltimore, Md. : 1950) 26 31748347
2022 Tetraspanin CD53 controls T cell immunity through regulation of CD45RO stability, mobility, and function. Cell reports 25 35767951
1995 Characterization of mouse CD53: epitope mapping, cellular distribution and induction by T cell receptor engagement during repertoire selection. European journal of immunology 25 7545113
2021 Identifying RBM47, HCK, CD53, TYROBP, and HAVCR2 as Hub Genes in Advanced Atherosclerotic Plaques by Network-Based Analysis and Validation. Frontiers in genetics 24 33519905
2002 CD53, a thymocyte selection marker whose induction requires a lower affinity TCR-MHC interaction than CD69, but is up-regulated with slower kinetics. International immunology 24 11867561
2010 A genome-wide linkage scan reveals CD53 as an important regulator of innate TNF-alpha levels. European journal of human genetics : EJHG 23 20407468
1998 Physiological activation of human neutrophils down-regulates CD53 cell surface antigen. Journal of leukocyte biology 23 9620662
2013 CD53, a suppressor of inflammatory cytokine production, is associated with population asthma risk via the functional promoter polymorphism -1560 C>T. Biochimica et biophysica acta 21 23313165
2004 LPS-induced CD53 expression: a protection mechanism against oxidative and radiation stress. Molecules and cells 20 15055538
2021 New immunological potential markers for triple negative breast cancer: IL18R1, CD53, TRIM, Jaw1, LTB, PTPRCAP. Discover oncology 19 35201443
2002 Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types. European journal of biochemistry 19 11846804
1993 Chromosomal localization of the Ox-44 (CD53) leukocyte antigen gene in man and rodents. Cytogenetics and cell genetics 16 8404042
1993 The genes for CD37, CD53, and R2, all members of a novel gene family, are located on different chromosomes. Immunogenetics 16 8436422
2022 The tetraspanin transmembrane protein CD53 mediates dyslipidemia and integrates inflammatory and metabolic signaling in hepatocytes. The Journal of biological chemistry 15 36581203
2020 Leukocyte Tetraspanin CD53 Restrains α3 Integrin Mobilization and Facilitates Cytoskeletal Remodeling and Transmigration in Mice. Journal of immunology (Baltimore, Md. : 1950) 14 32532837
2023 The tetraspanin CD53 protects stressed hematopoietic stem cells via promotion of DREAM complex-mediated quiescence. Blood 13 36542833
1995 Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, CD28, CD11a, CD44 and CD53. European journal of immunology 13 7533082
1993 Genomic structure of the human CD53 gene. Immunogenetics 13 8319976
2018 Association between CD53 genetic polymorphisms and tuberculosis cases. Genes & genomics 10 30506122
2007 The CD53 and CEACAM-1 genes are genetic targets for early B cell factor. European journal of immunology 9 17429843
1996 CD53 antigen and epidermal growth factor induce similar changes in the pattern of phorbol ester binding in a B cell lymphoma. Cellular immunology 9 8612282
2023 N-Glycosylation-dependent regulation of immune-specific tetraspanins CD37 and CD53. Biophysical journal 8 38031400
2015 Down-regulation of CD53 expression in Epinephelus coioides under LPS, poly (I:C), and cytokine stimulation. Fish & shellfish immunology 7 26631805
2003 Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primary cultures of mesangial cells. Kidney international 7 12631118
2021 Tetraspanin CD53 modulates lymphocyte trafficking but not systemic autoimmunity in Lyn-deficient mice. Immunology and cell biology 6 34514627
2025 Inhibition of CD53 Reduces the Formation of ROS-Induced Neutrophil Extracellular Traps and Protects Against Inflammatory Injury in Acute Pancreatitis. Journal of inflammation research 4 40098997
2024 Cutting Edge: The Tetraspanin CD53 Promotes CXCR4 Signaling and Bone Marrow Homing in B Cells. Journal of immunology (Baltimore, Md. : 1950) 3 38363205
2024 Tetraspanin CD53 regulates peripheral blood leucocytes vitality and pathogen infection in turbot (Scophthalmus maximus). Fish & shellfish immunology 2 38296007
2025 CTSS and CD53: Emerging m6A methylation markers in diabetic kidney disease pathogenesis and their clinical implications. BMC nephrology 1 40624623
2024 The conformation of tetraspanins CD53 and CD81 differentially affects their nanoscale organization and interaction with their partners. The Journal of biological chemistry 1 39159818
2026 Pathogenic implication of the CD53 tetraspanin in immune and cancer cells. Biochimica et biophysica acta. Molecular cell research 0 41921791

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