Affinage

CD53

Leukocyte surface antigen CD53 · UniProt P19397

Length
219 aa
Mass
24.3 kDa
Annotated
2026-04-28
57 papers in source corpus 32 papers cited in narrative 31 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CD53 is a leukocyte-enriched tetraspanin that organizes plasma membrane signaling platforms to regulate lymphocyte trafficking, activation, and innate immune cell inflammatory responses. It physically associates with MHC class II, integrins (α4β1, α3), L-selectin, IL-7Rα, CXCR4, and CD45, stabilizing these partners at the cell surface and modulating their signaling output: CD53 promotes IL-7R/PI3K/JAK-STAT signaling required for the pro-B to pre-B transition (PMID:31748347), stabilizes L-selectin to enable lymphocyte homing to lymph nodes (PMID:32428859), sustains CD45RO isoform expression and Lck activation for T cell proliferation and anti-tumor immunity (PMID:35767951), and facilitates CXCR4-driven B cell migration and bone marrow homing (PMID:38363205). Beyond adaptive immunity, CD53 ligation activates calcium flux, PKC, JNK, ERK, and AKT pathways in myeloid and mesangial cells (PMID:7511680, PMID:12606948, PMID:12631118), restrains inflammatory cytokine production via NF-κB suppression (PMID:23313165), promotes neutrophil transendothelial migration and NET formation (PMID:32532837, PMID:40098997), and maintains hematopoietic stem cell quiescence by scaffolding the PP2A–p130/DREAM complex (PMID:36542833).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1990 High

    Establishing CD53 as a founding tetraspanin resolved its four-transmembrane topology and N-glycosylation, providing the structural framework for understanding how it scaffolds membrane complexes.

    Evidence cDNA cloning and sequence analysis of human CD53 and its rat homologue OX-44

    PMID:1700763 PMID:2258620

    Open questions at the time
    • No crystal or cryo-EM structure of CD53
    • Stoichiometry of CD53 in membrane complexes unknown
  2. 1993 High

    Demonstrating that CD53 cross-linking triggers calcium flux and tyrosine kinase-dependent signaling established CD53 as an active signaling molecule rather than a passive structural component.

    Evidence Anti-CD53 F(ab')2 cross-linking on B cells, monocytes, granulocytes with calcium flux and pharmacological inhibitors; disulfide-dependent epitope mapping on extracellular loop

    PMID:7678222 PMID:8335905

    Open questions at the time
    • Identity of the proximal tyrosine kinase not determined
    • Physiological ligand unknown
  3. 1994 High

    Discovery that CD53 co-precipitates with MHC class II and other tetraspanins on B cells, and that its ligation induces PKC translocation, iNOS/NO production in macrophages, and B cell activation (G1 entry, Ig secretion), positioned CD53 as a central organizer of immune cell surface signaling platforms.

    Evidence Co-immunoprecipitation from B cell lysates; anti-CD53 cross-linking with PKC, iNOS, calcium, and B cell activation readouts

    PMID:7511680 PMID:7963560 PMID:8119731

    Open questions at the time
    • Whether CD53 directly contacts MHC class II or requires bridging tetraspanins not resolved
    • Relative contribution of PKC versus tyrosine kinase arms to macrophage NO production unclear
  4. 1996 High

    Reciprocal co-immunoprecipitation with integrin α4β1 and FRET-based proximity mapping with MHC class I/II and CD20 established that CD53 participates in multi-component supramolecular assemblies organized at nanometer scale on leukocyte surfaces.

    Evidence Reciprocal co-IP with mutant integrin dissection; flow cytometric FRET on B cells

    PMID:8757325 PMID:8816400

    Open questions at the time
    • Whether complexes are pre-formed or activation-dependent not fully resolved
    • Contribution of lipid composition to complex stability unknown
  5. 1997 Medium

    Pathway dissection of CD53-induced homotypic adhesion revealed that CD53 engages tyrosine kinase, PI3K, and PKC cascades to drive adhesion through both LFA-1-dependent and -independent routes, indicating CD53 can activate multiple downstream effector modules simultaneously.

    Evidence Anti-CD53 cross-linking with pharmacological inhibitors (genistein, wortmannin, PKC inhibitors); LAD patient cells lacking LFA-1; immunoelectron microscopy

    PMID:9225004 PMID:9241532 PMID:9514697

    Open questions at the time
    • LFA-1-independent adhesion molecule not identified
    • Whether CD53-induced adhesion occurs via inside-out integrin signaling not directly tested
  6. 2002 Medium

    Identification of JNK as a rapid downstream effector of CD53 ligation, independent of Vav, extended the signaling repertoire beyond calcium/PKC and linked CD53 to Jun-dependent transcriptional programs.

    Evidence In vitro JNK kinase assay after anti-CD53 cross-linking in B and T cell lymphoma lines; Jun-dependent reporter

    PMID:11846804

    Open questions at the time
    • Upstream kinase coupling CD53 to JNK not identified
    • In vivo relevance of CD53-JNK axis not tested
  7. 2003 High

    Connecting CD53 to AKT-mediated survival signaling (Bcl-xL upregulation, Bax downregulation) and ERK-dependent proliferation in mesangial cells broadened CD53 function beyond immune activation to include cytoprotection and mitogenesis.

    Evidence Anti-CD53 cross-linking with AKT phosphorylation, apoptosis readouts in lymphoma cells; ERK phosphorylation and thymidine incorporation with MEK inhibitor in mesangial cells

    PMID:12606948 PMID:12631118

    Open questions at the time
    • How CD53 selectively activates AKT versus ERK in different cell types not explained
    • Mesangial cell findings lack genetic loss-of-function validation
  8. 2007 High

    Localization of CD53 to macrophage intracellular plasma membrane compartments used by HIV-1 for budding revealed a role for CD53-enriched domains in pathogen biology, while identification of EBF1 as a transcriptional regulator placed CD53 expression under B-lineage developmental control.

    Evidence Immunoelectron microscopy and tracer accessibility in macrophages; EBF1 retroviral transduction and promoter analysis

    PMID:17429843 PMID:17438075

    Open questions at the time
    • Whether CD53 actively facilitates or restricts HIV budding not determined
    • Whether other transcription factors cooperate with EBF1 for CD53 induction not tested
  9. 2019 High

    Using Cd53-knockout mice and proximity ligation, the demonstration that CD53 physically stabilizes IL-7Rα to promote PI3K and JAK-STAT signaling provided the first in vivo genetic evidence that CD53 is essential for a specific developmental checkpoint — the pro-B to pre-B transition.

    Evidence Co-IP and PLA for CD53–IL-7Rα; phospho-flow; apoptosis assays; mixed bone marrow chimeras in Cd53−/− mice

    PMID:31748347

    Open questions at the time
    • Whether CD53 regulates IL-7Rα recycling or solely surface retention not distinguished
    • Structural basis of CD53–IL-7Rα interaction unknown
  10. 2020 High

    Cd53−/− mouse studies revealed that CD53 stabilizes L-selectin by restraining ADAM17-mediated shedding and modulates α3 integrin in neutrophils, establishing CD53 as a master regulator of leukocyte trafficking to lymph nodes and inflammatory sites.

    Evidence Cd53−/− adoptive transfer homing assays; ADAM17 inhibitor experiments; intravital microscopy; peritoneal inflammation and arthritis models

    PMID:32428859 PMID:32532837

    Open questions at the time
    • Direct binding interface between CD53 and L-selectin or ADAM17 not mapped
    • Whether CD53 physically sequesters ADAM17 or acts indirectly not resolved
  11. 2022 High

    Unbiased mass spectrometry identified CD45 as a key CD53 partner, and functional studies showed CD53 is required for CD45RO isoform expression, CD45 membrane mobility, and Lck activation — linking CD53 to the core TCR proximal signaling apparatus and explaining impaired anti-tumor T cell responses in Cd53−/− mice.

    Evidence Mass spectrometry; PLA; FRAP; phosphatase activity assay; in vivo tumor rejection in Cd53−/− mice

    PMID:35767951

    Open questions at the time
    • Whether CD53 influences CD45 alternative splicing or post-translational processing not distinguished
    • Mechanism by which CD53 controls CD45 membrane dynamics not elucidated
  12. 2022 Medium

    Extension of CD53 function beyond hematopoietic cells: CD53 deletion in hepatocytes attenuated Western diet-induced dyslipidemia and inflammatory gene expression, indicating CD53 integrates inflammatory and metabolic signals in non-immune cells.

    Evidence Germline CD53 KO mice on Western/NASH diets; primary hepatocyte cytokine and triglyceride assays

    PMID:36581203

    Open questions at the time
    • Cell-type-specific conditional KO not used; hepatocyte-autonomous role versus secondary immune effects not fully separated
    • Hepatocyte signaling partners of CD53 not identified
  13. 2023 High

    Discovery that CD53 scaffolds the PP2A–p130 interaction to stabilize the DREAM complex and promote HSC quiescence after inflammatory stress revealed a novel non-canonical tetraspanin function in stem cell biology.

    Evidence Proximity labeling proteomics; PP2A–p130 co-IP; Cd53−/− HSC cell cycle analysis after 5-FU stress

    PMID:36542833

    Open questions at the time
    • Whether CD53 directly binds PP2A or p130 not determined
    • Whether this mechanism operates in other quiescent cell types unknown
  14. 2023 Medium

    Glycosylation and conformational state emerged as regulatory layers: N-glycosylation of CD53 inhibits partner interactions (CD45, CD20, CD37) without affecting surface expression, and a closed-conformation mutant (F44E) shows enhanced clustering and CD45 binding, establishing that CD53 function is dynamically tunable.

    Evidence Glycosylation-site mutants with dSTORM super-resolution microscopy and co-IP; F44E conformational mutant analysis

    PMID:38031400 PMID:39159818

    Open questions at the time
    • Physiological signals that regulate CD53 glycosylation or conformational switching in vivo unknown
    • No structural data for open versus closed CD53 conformations
  15. 2024 High

    Identification of CXCR4 as a CD53 interaction partner and demonstration that CD53 promotes CXCR4 signaling and internalization extended the receptor-stabilization paradigm to chemokine receptors and explained impaired B cell bone marrow homing in Cd53−/− mice.

    Evidence PLA for CD53–CXCR4; CXCR4 phospho-flow and internalization; in vivo homing assay in Cd53−/− mice

    PMID:38363205

    Open questions at the time
    • Whether CD53 similarly modulates other chemokine receptors not tested
    • Mechanism of CXCR4 internalization enhancement not defined

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the identity of a physiological extracellular ligand for CD53, the atomic structure of CD53 and its partner complexes, the signals controlling conformational switching in vivo, and whether the receptor-stabilization mechanism generalizes across all CD53 partners.
  • No physiological ligand identified
  • No high-resolution structure available
  • In vivo regulation of CD53 conformation and glycosylation state unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 8 GO:0098772 molecular function regulator activity 4
Localization
GO:0005886 plasma membrane 8 GO:0031410 cytoplasmic vesicle 1
Pathway
R-HSA-168256 Immune System 12 R-HSA-162582 Signal Transduction 8 R-HSA-1500931 Cell-Cell communication 4 R-HSA-1266738 Developmental Biology 2 R-HSA-5357801 Programmed Cell Death 2
Partners
CD45CD81CXCR4IL7RITGA4PPP2CARBL2SELL
Complex memberships
MHC class II–tetraspanin complexPP2A–p130/DREAM complexTetraspanin-enriched microdomain (TEM)

Evidence

Reading pass · 31 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1990 CD53 is a 219-amino acid type III integral membrane protein with four transmembrane domains and N-glycosylation sites, constituting the structural basis of the tetraspanin family; it is the human homologue of rat OX-44. cDNA cloning, sequence analysis, immunoprecipitation of transfected cells Journal of immunology High 1700763 2258620
1993 Cross-linking of CD53 on human B cells, monocytes, and granulocytes triggers cytoplasmic calcium fluxes and activates the monocyte oxidative burst; signaling is largely independent of protein kinase C but sensitive to high-dose staurosporine and dependent on tyrosine kinases rather than GTP-binding proteins. Cross-linking of cell-bound F(ab')2 anti-CD53 mAb; calcium flux assay; oxidative burst assay; pharmacological inhibitors (staurosporine, ADP-ribosylating toxins) Journal of immunology High 8335905
1993 The major extracellular (hydrophilic) loop of CD53 contains epitopes recognized by anti-CD53 mAbs (OX-44, 2D1, 6E2, 7D2), and these epitopes are sensitive to reduction, demonstrating the importance of disulfide bonding in the correct folding of the CD53 extracellular domain and directly confirming the four-transmembrane topology. Epitope mapping using GST-fusion chimeric proteins and anti-rat CD53 mAbs; reduction sensitivity assays European journal of immunology High 7678222
1994 CD53 (together with CD37, TAPA-1/CD81, and R2/C33) co-precipitates with MHC class II (DR) glycoproteins from B cell lysates in mild detergent, forming large multicomponent complexes on the B cell surface. Co-immunoprecipitation and preclearing experiments from human B-cell line and tonsillar B cell lysates Immunogenetics High 8119731
1994 Activation of CD53 in rat macrophages by antibody cross-linking increases inositol phosphates, diacylglycerol, and intracellular Ca2+ (insensitive to pertussis/cholera toxins), leads to protein kinase C translocation to the membrane, and induces expression of inducible nitric oxide synthase (iNOS) resulting in nitric oxide release in a PKC- and protein synthesis-dependent manner. Antibody cross-linking; inositol phosphate and diacylglycerol measurement; Ca2+ mobilization; PKC translocation assay; NO measurement; iNOS expression analysis; pharmacological inhibitors The Journal of experimental medicine High 7511680
1994 Cross-linking of CD53 on resting human B lymphocytes promotes their entry into G1 phase (increased CD69, cell volume, RNA synthesis, c-myc levels), and in combination with cytokines (IL-2, IL-4) induces DNA synthesis and immunoglobulin production. Anti-CD53 mAb cross-linking; flow cytometry; RNA synthesis measurement; c-myc immunoblot; [3H]thymidine incorporation; Ig ELISA Journal of immunology High 7963560
1995 Immune complexes of rat CD53 contain tyrosine phosphatase activity capable of dephosphorylating Lck in vitro; the phosphatase is distinct from CD45 and is inhibited by tyrosine phosphatase inhibitors. CD63 similarly co-precipitates a phosphatase. Immunoprecipitation from lymph node and thymoma lysates; in vitro phosphatase activity assay on phospho-Lck and synthetic substrate; depletion experiments; phosphatase inhibitor treatment European journal of immunology Medium 7621882
1996 CD53, CD82, CD63, and CD81 specifically associate with integrin α4β1 (VLA-4/CD49d/CD29) on hematopoietic cell lines, as shown by reciprocal co-immunoprecipitation; the association is independent of the α4 cytoplasmic domain and divalent cations but is lost in two α4 adhesion-deficient mutants (D346E and D408E). Reciprocal co-immunoprecipitation; confocal microscopy; mutant integrin transfectants; divalent cation chelation Journal of immunology High 8757325
1996 CD53, CD81, and CD82 are in close proximity (2–10 nm) to MHC class II (DR, DQ), MHC class I, and CD20 on B cell surfaces, forming supramolecular complexes; FRET analysis suggests all these molecules participate in a single multi-component assembly. Flow cytometric FRET (fluorescence energy transfer) with fluorescently labeled mAbs Journal of immunology High 8816400
1997 CD53 ligation on lymphoma B cells (rat IR938F) induces homotypic adhesion that requires divalent cations (Ca2+, Mg2+), de novo protein synthesis, and is mediated by tyrosine kinase (genistein/piceatannol-sensitive), PI3K (wortmannin-sensitive), and PKC (H7/bisindolylmaleimide-sensitive) signaling pathways, but is LFA-1-independent; electron microscopy reveals localized membrane contact zones. Anti-CD53 mAb cross-linking; cell aggregation assay; pharmacological inhibitors (genistein, piceatannol, wortmannin, H7, bisindolylmaleimide, cycloheximide, actinomycin D, EGTA/EDTA); immunoelectron microscopy Cellular immunology High 9225004
1997 Anti-CD53 mAb induces homotypic aggregation of lymphoid cell lines via both LFA-1/ICAM-1-dependent and -independent pathways; aggregation is energy-dependent and partially blocked by anti-LFA-1 or anti-ICAM-1, but not by anti-CD44 or anti-CD49d mAbs. Anti-CD53 mAb cross-linking; blocking mAb experiments; cell aggregation assay including a LAD patient B cell line lacking LFA-1 Immunobiology Medium 9241532
1997 Anti-CD53 mAb (like anti-CD9, anti-CD81, anti-CD82 mAbs) delivers a costimulatory signal for CD3-mediated T cell activation and inhibits migration of megakaryocytic and pre-B cell lines, consistent with CD53 participation in a tetraspan network. mAb cross-linking; T cell costimulation assay; homotypic aggregation and migration inhibition assays in HEL and NALM-6 cell lines Cellular immunology Medium 9514697
1998 Physiological neutrophil activators (TNFα, PAF, fMLP, PMA, ionomycin) cause down-regulation of CD53 from the neutrophil cell surface via a proteolytic mechanism (inhibited by PMSFP), without changes in CD53 transcript levels. Flow cytometry; immunoblotting; pharmacological inhibition (PMSF); Northern/RT analysis Journal of leukocyte biology Medium 9620662
2002 Ligation of CD53 (rat or human) induces a transient 3–4-fold activation of JNK that peaks at 3–5 min in B-cell lymphoma and T-cell lymphoma lines, and in a renal carcinoma line transiently transfected with human CD53; this JNK activation is independent of Vav and sufficient to stimulate Jun-dependent transcription. Anti-CD53 mAb cross-linking; in vitro JNK kinase assay (endogenous and exogenous); Jun-dependent transcriptional reporter; Vav co-stimulation assay European journal of biochemistry Medium 11846804
2003 CD53 ligation on tumor B and T cells (IR938F, Jurkat) reduces apoptosis induced by serum deprivation, decreases caspase activation and DNA fragmentation, increases Bcl-xL and decreases Bax, and activates AKT (phosphorylation at Ser473). Anti-CD53 mAb cross-linking; PARP cleavage assay; DNA fragmentation assay; Bcl-xL/Bax immunoblot; AKT phosphorylation immunoblot Oncogene High 12606948
2003 CD53 ligation in rat mesangial cells triggers DNA synthesis via ERK1/ERK2 activation (blocked by PD98059/MEK inhibitor), but not through PI3K, PKC, or calcium channel pathways; CD53 is expressed in mesangial cells in vivo. Anti-CD53 mAb cross-linking; [3H]thymidine incorporation; ERK phosphorylation by immunoblot; pharmacological inhibitors (PD98059, wortmannin, PKC inhibitors, thapsigargin, verapamil); flow cytometry for apoptosis Kidney international Medium 12631118
2004 CD53 overexpression in macrophages increases intracellular GSH, lowers peroxide levels, and confers resistance to H2O2 and UVB irradiation; antisense CD53 has the opposite effects; CD53 is induced by LPS and nitric oxide (SNAP) in macrophages. Stable sense and antisense CD53 transfection; GSH measurement; peroxide assay; H2O2 and UVB cell viability assays; Northern blot; microarray Molecules and cells Medium 15055538
2007 In macrophages, HIV-1 buds into an intracellular plasma membrane domain containing tetraspanins CD81, CD9, and CD53 (not classic endosomes); this compartment is connected to the cell surface (accessible to extracellular tracers at 4°C) and lacks endosome markers including CD63. Immunofluorescence microscopy; immunoelectron microscopy; horseradish peroxidase and ruthenium red tracer accessibility assays; marker co-localization The Journal of cell biology High 17438075
2007 CD53 gene expression is directly regulated by the transcription factor EBF1; functional EBF1 binding sites are present in the CD53 promoter and EBF1 expression drives CD53 transcription in transient transfection assays. Retroviral EBF1 transduction of BaF/3 cells; microarray; promoter 5' end mapping; transient transfection transcriptional assays European journal of immunology Medium 17429843
2013 CD53 knockdown by siRNA in THP-1 monocytic cells stimulated with house dust mite significantly increases production of inflammatory cytokines and NFκB activity, demonstrating that CD53 suppresses over-activation of inflammatory cytokine responses. siRNA knockdown; cytokine ELISA; NFκB activity assay Biochimica et biophysica acta Medium 23313165
2014 CD53 ligation on rat NK cells reduces degranulation and IFNγ responses to activating NK receptors (Ly49s3, NKR-P1A, NKp46), induces activation of the β2 integrin LFA-1, promotes homotypic NK cell adhesion, and enhances NK cell proliferation in response to IL-2. Anti-CD53 mAb cross-linking; degranulation assay; redirected killing assay; IFNγ ELISA; LFA-1 activation flow cytometry; proliferation assay PloS one Medium 24832104
2019 CD53 physically interacts with IL-7Rα and promotes IL-7 signaling (PI3K and JAK/STAT pathways) in developing B cells; loss of CD53 reduces IL-7Rα surface expression, increases apoptosis in pro-B cells, and blocks the pro-B to pre-B transition. Co-immunoprecipitation; proximity ligation assay; flow cytometry for IL-7Rα; phospho-flow for PI3K and JAK/STAT; apoptosis assays; mixed bone marrow chimeras Journal of immunology High 31748347
2020 CD53 stabilizes L-selectin surface expression on B and T cells, restrains L-selectin shedding via both ADAM17-dependent and ADAM17-independent mechanisms, and is required for efficient lymphocyte homing to lymph nodes; Cd53−/− mice have markedly smaller lymph nodes due to reduced B and T cell homing. Cd53−/− mouse analysis; flow cytometry for L-selectin; adoptive transfer homing assays; ADAM17 inhibitor experiments; in vivo lymph node cellularity iScience High 32428859
2020 CD53 restrains α3 integrin mobilization in neutrophils, promotes cytoskeletal remodeling and transendothelial migration; Cd53−/− neutrophils show defective transmigration across endothelium in response to TNF, CXCL1, and CCL2, and near-complete absence of L-selectin. Cd53−/− mouse; intravital microscopy; peritoneal inflammation model; flow cytometry for adhesion molecules and integrins; cytoskeletal remodeling assays; serum-induced arthritis model Journal of immunology High 32532837
2022 CD53 interacts with CD45 (identified by unbiased mass spectrometry) and is required for CD45RO isoform expression, CD45 membrane stability and mobility, and optimal CD45 phosphatase activity, leading to Lck activation; Cd53−/− T cells show proliferation defects and impaired tumor rejection. Unbiased mass spectrometry; proximity ligation assay; FRAP (fluorescence recovery after photobleaching); phosphatase activity assay; Lck activation immunoblot; in vivo tumor rejection model; IFNγ ELISPOT Cell reports High 35767951
2022 CD53 integrates inflammatory and metabolic signals in hepatocytes; CD53 deletion blocks Western diet-induced dyslipidemia, hepatic inflammatory gene expression, and triglyceride accumulation; CD53 deletion attenuates TNFα- and fatty acid+LPS-induced cytokine expression in isolated hepatocytes. Germline CD53 KO mice; Western diet feeding; NASH diet model; primary hepatocyte isolation; cytokine gene expression; triglyceride assay; glucose transporter 8 deletion and trehalose treatment as comparators The Journal of biological chemistry Medium 36581203
2023 CD53 interacts with DREAM complex-associated proteins, specifically promoting the interaction between Rbl2/p130 and its phosphatase PP2A, stabilizing p130 for DREAM complex assembly; this promotes HSC quiescence following inflammatory stress, and loss of CD53 leads to prolonged cycling and reduced HSC function. Proximity labeling proteomics; confocal fluorescence microscopy; DREAM complex co-immunoprecipitation; PP2A-p130 interaction assay; Cd53−/− HSC cell cycle analysis; 5-FU stress model Blood High 36542833
2023 Glycosylation of CD53 inhibits its interaction with partner proteins CD45, CD20, and CD37; abrogation of CD53 glycosylation does not affect surface expression but increases interaction with partners. CD37 glycosylation is required for its surface expression, while CD53 glycosylation controls partner protein interaction capacity. Glycosylation mutant generation; surface expression flow cytometry; single-molecule dSTORM super-resolution microscopy; co-immunoprecipitation Biophysical journal Medium 38031400
2024 CD53 physically interacts with CXCR4 (confirmed by proximity ligation assay) and promotes CXCL12-induced CXCR4 signaling and receptor internalization; Cd53−/− B cells show reduced CXCL12 migration in vitro and impaired bone marrow homing in vivo. Proximity ligation assay; in vitro migration assay; CXCR4 signaling (phospho-flow); CXCR4 internalization assay; in vivo homing assay in Cd53−/− mice Journal of immunology High 38363205
2024 The 'closed' conformational mutant of CD53 (F44E) shows increased clustering in nanodomains and greater interaction with its partner CD45 compared to wild-type CD53; absence of CD53 increases CD45 clustering tendency, indicating CD53 conformation regulates its nanoscale membrane organization and partner interactions. Conformational mutant generation (F44E); super-resolution dSTORM microscopy; co-immunoprecipitation; CD53 KO B cells The Journal of biological chemistry Medium 39159818
2025 CD53 promotes ROS-induced neutrophil extracellular trap (NET) formation via modulation of the PI3K/AKT pathway; anti-CD53 neutralizing antibody inhibits NET formation in vitro and reduces inflammatory injury in a caerulein-induced acute pancreatitis mouse model. PMA-induced in vitro NET model; anti-CD53 neutralizing antibody; PI3K/AKT pathway analysis; caerulein AP mouse model; NET quantification in tissues Journal of inflammation research Medium 40098997

Source papers

Stage 0 corpus · 57 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2007 In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53. The Journal of cell biology 265 17438075
1996 Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29). Journal of immunology (Baltimore, Md. : 1950) 200 8757325
1996 Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. Journal of immunology (Baltimore, Md. : 1950) 177 8816400
1994 Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/C33) with MHC class II glycoproteins. Immunogenetics 165 8119731
1997 Functional analysis of four tetraspans, CD9, CD53, CD81, and CD82, suggests a common role in costimulation, cell adhesion, and migration: only CD9 upregulates HB-EGF activity. Cellular immunology 127 9514697
1992 C33 antigen recognized by monoclonal antibodies inhibitory to human T cell leukemia virus type 1-induced syncytium formation is a member of a new family of transmembrane proteins including CD9, CD37, CD53, and CD63. Journal of immunology (Baltimore, Md. : 1950) 115 1401919
1990 The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens. Immunogenetics 73 1700763
1990 Identification and analysis of cDNA clones encoding CD53. A pan-leukocyte antigen related to membrane transport proteins. Journal of immunology (Baltimore, Md. : 1950) 69 2258620
1993 CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. Journal of immunology (Baltimore, Md. : 1950) 68 8335905
2006 The effects of intensive, moderate and downhill treadmill running on human blood lymphocytes expressing the adhesion/activation molecules CD54 (ICAM-1), CD18 (beta2 integrin) and CD53. European journal of applied physiology 67 16506060
2020 Tetraspanin CD53: an overlooked regulator of immune cell function. Medical microbiology and immunology 51 32440787
1994 Induction of nitric oxide release by MRC OX-44 (anti-CD53) through a protein kinase C-dependent pathway in rat macrophages. The Journal of experimental medicine 51 7511680
2003 Apoptosis protection and survival signal by the CD53 tetraspanin antigen. Oncogene 49 12606948
1997 Recurrent infectious diseases in human CD53 deficiency. Clinical and diagnostic laboratory immunology 48 9067662
1995 Association of the transmembrane 4 superfamily molecule CD53 with a tyrosine phosphatase activity. European journal of immunology 44 7621882
2017 Pathogen lineage-based genome-wide association study identified CD53 as susceptible locus in tuberculosis. Journal of human genetics 43 28878339
2013 Characterization of tetraspanins CD9, CD53, CD63, and CD81 in monocytes and macrophages in HIV-1 infection. Journal of leukocyte biology 43 23570947
1997 Expression of tetra-spans transmembrane family (CD9, CD37, CD53, CD63, CD81 and CD82) in normal and neoplastic human keratinocytes: an association of CD9 with alpha 3 beta 1 integrin. The British journal of dermatology 39 9470900
1993 Epitope mapping of anti-rat CD53 monoclonal antibodies. Implications for the membrane orientation of the Transmembrane 4 Superfamily. European journal of immunology 38 7678222
1993 Gene structure, chromosomal localization, and protein sequence of mouse CD53 (Cd53): evidence that the transmembrane 4 superfamily arose by gene duplication. International immunology 38 8452817
2000 Increased expression of the tetraspanins CD53 and CD63 on apoptotic human neutrophils. Journal of leukocyte biology 34 10733097
2020 Tetraspanin CD53 Promotes Lymphocyte Recirculation by Stabilizing L-Selectin Surface Expression. iScience 31 32428859
1994 Cross-linking of CD53 promotes activation of resting human B lymphocytes. Journal of immunology (Baltimore, Md. : 1950) 31 7963560
2014 The tetraspanin CD53 modulates responses from activating NK cell receptors, promoting LFA-1 activation and dampening NK cell effector functions. PloS one 30 24832104
1997 Ligation of CD53/OX44, a tetraspan antigen, induces homotypic adhesion mediated by specific cell-cell interactions. Cellular immunology 26 9225004
1997 Anti-CD53 monoclonal antibody induced LFA-1/ICAM-1-dependent and -independent lymphocyte homotypic cell aggregation. Immunobiology 26 9241532
2019 The Tetraspanin CD53 Regulates Early B Cell Development by Promoting IL-7R Signaling. Journal of immunology (Baltimore, Md. : 1950) 25 31748347
1995 Characterization of mouse CD53: epitope mapping, cellular distribution and induction by T cell receptor engagement during repertoire selection. European journal of immunology 25 7545113
2022 Tetraspanin CD53 controls T cell immunity through regulation of CD45RO stability, mobility, and function. Cell reports 24 35767951
2021 Identifying RBM47, HCK, CD53, TYROBP, and HAVCR2 as Hub Genes in Advanced Atherosclerotic Plaques by Network-Based Analysis and Validation. Frontiers in genetics 24 33519905
2002 CD53, a thymocyte selection marker whose induction requires a lower affinity TCR-MHC interaction than CD69, but is up-regulated with slower kinetics. International immunology 24 11867561
2010 A genome-wide linkage scan reveals CD53 as an important regulator of innate TNF-alpha levels. European journal of human genetics : EJHG 23 20407468
1998 Physiological activation of human neutrophils down-regulates CD53 cell surface antigen. Journal of leukocyte biology 22 9620662
2013 CD53, a suppressor of inflammatory cytokine production, is associated with population asthma risk via the functional promoter polymorphism -1560 C>T. Biochimica et biophysica acta 21 23313165
2004 LPS-induced CD53 expression: a protection mechanism against oxidative and radiation stress. Molecules and cells 20 15055538
2021 New immunological potential markers for triple negative breast cancer: IL18R1, CD53, TRIM, Jaw1, LTB, PTPRCAP. Discover oncology 18 35201443
2002 Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types. European journal of biochemistry 18 11846804
2022 The tetraspanin transmembrane protein CD53 mediates dyslipidemia and integrates inflammatory and metabolic signaling in hepatocytes. The Journal of biological chemistry 15 36581203
1993 Chromosomal localization of the Ox-44 (CD53) leukocyte antigen gene in man and rodents. Cytogenetics and cell genetics 15 8404042
1993 The genes for CD37, CD53, and R2, all members of a novel gene family, are located on different chromosomes. Immunogenetics 15 8436422
2020 Leukocyte Tetraspanin CD53 Restrains α3 Integrin Mobilization and Facilitates Cytoskeletal Remodeling and Transmigration in Mice. Journal of immunology (Baltimore, Md. : 1950) 12 32532837
1995 Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, CD28, CD11a, CD44 and CD53. European journal of immunology 12 7533082
1993 Genomic structure of the human CD53 gene. Immunogenetics 12 8319976
2023 The tetraspanin CD53 protects stressed hematopoietic stem cells via promotion of DREAM complex-mediated quiescence. Blood 11 36542833
2018 Association between CD53 genetic polymorphisms and tuberculosis cases. Genes & genomics 10 30506122
2007 The CD53 and CEACAM-1 genes are genetic targets for early B cell factor. European journal of immunology 8 17429843
1996 CD53 antigen and epidermal growth factor induce similar changes in the pattern of phorbol ester binding in a B cell lymphoma. Cellular immunology 8 8612282
2023 N-Glycosylation-dependent regulation of immune-specific tetraspanins CD37 and CD53. Biophysical journal 7 38031400
2015 Down-regulation of CD53 expression in Epinephelus coioides under LPS, poly (I:C), and cytokine stimulation. Fish & shellfish immunology 7 26631805
2003 Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primary cultures of mesangial cells. Kidney international 6 12631118
2021 Tetraspanin CD53 modulates lymphocyte trafficking but not systemic autoimmunity in Lyn-deficient mice. Immunology and cell biology 5 34514627
2025 Inhibition of CD53 Reduces the Formation of ROS-Induced Neutrophil Extracellular Traps and Protects Against Inflammatory Injury in Acute Pancreatitis. Journal of inflammation research 3 40098997
2024 Tetraspanin CD53 regulates peripheral blood leucocytes vitality and pathogen infection in turbot (Scophthalmus maximus). Fish & shellfish immunology 2 38296007
2025 CTSS and CD53: Emerging m6A methylation markers in diabetic kidney disease pathogenesis and their clinical implications. BMC nephrology 1 40624623
2026 Pathogenic implication of the CD53 tetraspanin in immune and cancer cells. Biochimica et biophysica acta. Molecular cell research 0 41921791
2024 Cutting Edge: The Tetraspanin CD53 Promotes CXCR4 Signaling and Bone Marrow Homing in B Cells. Journal of immunology (Baltimore, Md. : 1950) 0 38363205
2024 The conformation of tetraspanins CD53 and CD81 differentially affects their nanoscale organization and interaction with their partners. The Journal of biological chemistry 0 39159818