Affinage

BCAR1

Breast cancer anti-estrogen resistance protein 1 · UniProt P56945

Length
870 aa
Mass
93.4 kDa
Annotated
2026-06-09
100 papers in source corpus 53 papers cited in narrative 53 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

BCAR1/p130Cas is a multidomain scaffold protein that converts integrin and growth-factor engagement into actin-based cell motility by nucleating a tyrosine-phosphorylation-dependent signaling complex at focal adhesions (PMID:7479864, PMID:7545676, PMID:20430882). Its N-terminal SH3 domain binds the proline-rich region of FAK (APPKPSR, 711-717), while a C-terminal RPLPSPP motif and Tyr762 engage the SH3/SH2 domains of Src; both the SH3 and C-terminal Cas-family-homology (CCH) domains are required for focal-adhesion targeting and for promoting migration (PMID:7479864, PMID:8621540, PMID:20430882). Within the Src–FAK–p130Cas complex, Src kinase activity phosphorylates the central substrate-domain YXXP repeats, creating docking sites for the Crk family and other SH2 adaptors, which is sufficient to activate Rac1 and drive membrane ruffling, lamellipodium formation, and leading-edge actin flux (PMID:8621540, PMID:17038317, PMID:18793427, PMID:20976150). Genetic ablation establishes these functions as physiological: p130Cas-null mice die in utero with cardiovascular defects and disorganized myofibrils, and null fibroblasts have impaired stress-fiber formation and severe defects in spreading and migration that are rescued by re-expression (PMID:9697697, PMID:10448062). p130Cas operates as a mechanosensor, undergoing stretch- and rigidity-dependent tyrosine phosphorylation that drives cell reorientation and, via filopodia-tip recruitment through its CCHD, regulates filopodia stability (PMID:16597701, PMID:30639111, PMID:22178114). The same scaffold transduces signals from diverse upstream inputs—it is phosphorylated downstream of Pyk2/RAFTK, Ack1, Bmx, NPM-ALK, EphA2, and netrin-1, and forms estrogen-induced ERα–c-Src–PI3K complexes that drive ERK1/2 activation and cyclin D1 induction (PMID:9242628, PMID:12832404, PMID:15020686, PMID:16105984, PMID:17038317, PMID:17251438). Its phosphorylation state is set by phosphatases LAR, SAP-1, and PTPN14 (the latter acting specifically at Tyr128), and the protein is cleaved by caspase-3 at DVPD416 and DSPD748 during apoptosis, dismantling focal adhesions (PMID:10320483, PMID:11278335, PMID:22710723, PMID:10712510). p130Cas is oncogenic in breast and HER2/ErbB2-driven contexts, mediates antiestrogen resistance through binding partner BCAR3, and is a synthetic-lethal dependency in mutant-KRAS pancreatic cancer (PMID:10639512, PMID:16651418, PMID:20505116, PMID:24584939, PMID:34192548). Beyond migration and transformation, nuclear p130Cas alleviates NF-κB activity in osteocytes to restrain RANKL-driven bone resorption (PMID:31579816).

Mechanistic history

Synthesis pass · year-by-year structured walk · 24 steps
  1. 1995 High

    Established the founding physical link between p130Cas and the focal-adhesion machinery, defining how the scaffold is recruited and how adhesion controls its activation state.

    Evidence Yeast two-hybrid and co-IP mapping the SH3-FAK interaction; integrin-adhesion-dependent tyrosine phosphorylation blocked by cytochalasin D

    PMID:7479864 PMID:7545676

    Open questions at the time
    • Did not identify the kinase responsible for adhesion-induced phosphorylation
    • Did not define downstream effectors of phosphorylated p130Cas
  2. 1996 High

    Resolved the modular architecture of the scaffold by mapping FAK, Src, and Crk binding to distinct phosphorylation-state-dependent sites, explaining how one protein integrates multiple partners.

    Evidence GST pulldowns and mutagenesis mapping RPLPSPP/Tyr762 (Src) and substrate-domain (Crk) sites

    PMID:8621540 PMID:8649789

    Open questions at the time
    • In vitro binding without cellular validation of all sites
    • Did not establish functional consequence of each interaction
  3. 1997 High

    Identified the kinases that phosphorylate p130Cas, showing FAK, Src, and Pyk2/RAFTK cooperate to fully phosphorylate the substrate domain and recruit downstream adaptors.

    Evidence In vitro kinase assays with purified domains, Tyr402 mutagenesis, fibronectin stimulation, and Nck recruitment co-IP

    PMID:9032297 PMID:9242628

    Open questions at the time
    • Relative in-cell contribution of each kinase not quantified
    • Did not link phosphorylation to a motility phenotype
  4. 1997 High

    Demonstrated that pathogen-driven phosphatase activity (Yersinia YopH) dephosphorylates and disassembles the FAK–p130Cas focal complex, establishing the complex as a target for host-pathogen manipulation.

    Evidence Active vs inactive YopH comparison, co-localization, and PTPase assays in HeLa cells

    PMID:9171345

    Open questions at the time
    • Did not identify endogenous mammalian phosphatases
    • Mechanism of uptake downstream of complex not detailed
  5. 1998 High

    Proved p130Cas is functionally required for FAK-promoted migration and for organismal development, moving the scaffold from a binding partner to a causal motility/cytoskeletal effector.

    Evidence FAK P712/715A mutant migration assays, SH3 dominant-negative, and germline knockout mouse with cardiovascular/cytoskeletal phenotypes

    PMID:9425168 PMID:9697697

    Open questions at the time
    • Embryonic lethality limited tissue-specific dissection
    • Did not isolate which downstream effector drives migration
  6. 1998 Medium

    Showed p130Cas is a convergence node for GPCR and cytokine receptor inputs, nucleating large multiprotein signaling complexes coupled to JNK activation.

    Evidence Co-IP and inhibitor studies in angiotensin-II-stimulated VSMC and growth-hormone-stimulated cells

    PMID:9648724 PMID:9837978

    Open questions at the time
    • Co-IP-based complexes lack mutagenesis confirmation
    • Direct versus indirect associations not distinguished
  7. 1999 High

    Confirmed p130Cas is a bona fide phosphatase substrate (LAR) controlling its stability and adhesion localization, and dissociated its motility role from PTEN-dependent growth suppression.

    Evidence Substrate-trapping LAR mutants with rescue; in vitro PTEN phosphatase assay (negative) plus invasion rescue

    PMID:10320483 PMID:9927060

    Open questions at the time
    • How dephosphorylation triggers degradation not defined
    • PTEN effect shown to be indirect, leaving the relevant phosphatase open
  8. 1999 High

    Provided definitive knockout/rescue evidence that p130Cas drives cell spreading and motility, and linked it to Cdc42 activation downstream of MCSP.

    Evidence Knockout fibroblast re-expression with wound-healing/transwell assays; MCSP-Ack1-Cdc42 dominant-negative spreading assays

    PMID:10448062 PMID:10587647

    Open questions at the time
    • Did not resolve which adaptor links p130Cas to the GTPase
    • Cdc42 link based on dominant negatives without direct kinase assay
  9. 2000 High

    Expanded the partner repertoire (PI3K-p85, AND-34/BCAR3) and identified BCAR1 as the human gene conferring antiestrogen resistance, anchoring its cancer relevance.

    Evidence Domain-mapped co-IP for p85; GEF assays for AND-34; retroviral mutagenesis/cDNA rescue identifying the BCAR1 locus

    PMID:10639512 PMID:10799562 PMID:10896938

    Open questions at the time
    • Mechanism coupling p130Cas to antiestrogen resistance not yet defined
    • GEF regulation shown only in cells without reconstitution
  10. 2000 High

    Defined how p130Cas is dismantled during apoptosis, identifying caspase-3 cleavage sites that drive focal-adhesion disassembly.

    Evidence In vitro caspase cleavage with DVPD416/DSPD748 point mutagenesis and immunofluorescence

    PMID:10712510

    Open questions at the time
    • Functional consequence of cleavage fragments not fully established here
    • Did not test other proteases
  11. 2001 Medium

    Positioned p130Cas as a hub linking integrins to RTK transactivation and to additional phosphatase/adaptor regulators in adhesion and cardiac contexts.

    Evidence Co-IP of integrin-induced p130Cas-Src-EGFR complex; SAP-1 substrate-trapping; SHIP2 SH2-mutant studies; FAK/Cas Z-line localization

    PMID:11158326 PMID:11278335 PMID:11514617 PMID:11756413

    Open questions at the time
    • Several interactions rest on single-lab co-IP
    • SAP-1 is High-confidence but in-vivo substrate specificity not broadly tested
  12. 2003 Medium

    Solidified the p130Cas-CrkII complex as a migration switch and added kinases (Bmx) and regulators (p140Cap, KAI1/CD82) that tune complex formation and protein level.

    Evidence Co-IP, dominant negatives, and overexpression rescue in migration/ruffling assays; affinity-MS identification of p140Cap

    PMID:12738793 PMID:12832404 PMID:14657239

    Open questions at the time
    • Mechanisms largely correlative co-IP without reconstitution
    • Direct vs indirect kinase action on p130Cas not fully resolved
  13. 2004 High

    Established p130Cas as a transducer of non-genomic estrogen signaling and characterized its proteolytic processing during anoikis.

    Evidence Rapid ERα-Src-p85 co-IP with siRNA/kinase readouts; anoikis cleavage-fragment overexpression with caspase/calpain inhibitors

    PMID:14743392 PMID:15020686

    Open questions at the time
    • Direct ERα-p130Cas contact versus Src-bridged not resolved
    • Anoikis proteases incompletely identified (Medium-confidence)
  14. 2005 High

    Identified upstream localization machinery (Ajuba) and demonstrated p130Cas oncogenicity in vivo, showing overexpression drives mammary hyperplasia and accelerates HER2-Neu tumors.

    Evidence Ajuba-null fibroblasts with FRET Rac readouts; MMTV-p130Cas and double-transgenic mouse tumor models with siRNA

    PMID:15728191 PMID:16651418

    Open questions at the time
    • How Ajuba targets p130Cas to nascent adhesions not molecularly defined
    • Whether mammary phenotype is cell-autonomous to p130Cas signaling not isolated
  15. 2006 High

    Established p130Cas as essential for oncogenic-kinase transformation (NPM-ALK), mechanotransduction (rigidity sensing via Fyn), and pathogen invasion via its YXXP substrate domain.

    Evidence Cas-/- reconstitution for NPM-ALK transformation and Salmonella invasion; Fyn-dependent leading-edge phosphorylation in rigidity assays; Ack1-Cdc42-Crk complex with siRNA migration readout

    PMID:16105984 PMID:16597701 PMID:16914515 PMID:17038317

    Open questions at the time
    • Force-dependent Fyn phosphorylation inferred rather than directly measured
    • Substrate-domain requirement defined but specific YXXP residues not all mapped
  16. 2007 High

    Extended p130Cas function to neuronal guidance, placing it downstream of Src kinases and upstream of Rac1/Cdc42 in netrin-1-driven axon attraction in vivo.

    Evidence Biochemical epistasis and in vivo RNAi of commissural axon projection

    PMID:17251438

    Open questions at the time
    • Receptor coupling p130Cas to netrin signaling not identified
    • Effector adaptor in neurons not defined
  17. 2008 High

    Demonstrated that p130Cas substrate-domain phosphorylation by Src (not FAK) is sufficient to activate Rac1, and uncovered a growth-control role through Smad3-dependent suppression of TGF-β arrest.

    Evidence Functional Interaction Trap selective phosphorylation with Rac1 assays; p130Cas-Smad3 co-IP with loss/gain function and cell-cycle readouts

    PMID:18321991 PMID:18793427

    Open questions at the time
    • How phospho-p130Cas reduces Smad3 phosphorylation mechanistically unclear
    • FAK contribution to Rac activation context-dependent
  18. 2010 High

    Dissected the structure-function logic of the scaffold, showing both SH3 and CCH domains are needed for adhesion targeting and that substrate-domain and Src-binding-domain phosphorylation have separable roles in actin flux versus sustained adhesion turnover; also established p130Cas as essential for ErbB2 transformation.

    Evidence FRAP/live imaging with deletion mutants; Cas-/- MEF rescue with domain mutants and quantitative FA dynamics; ErbB2 complex co-IP with siRNA and in vivo tumor assays

    PMID:20430882 PMID:20505116 PMID:20976150

    Open questions at the time
    • Molecular basis of the phosphorylation-independent SBD function unresolved
    • How CCHD contributes to targeting beyond SH3-FAK not defined
  19. 2011 Medium

    Broadened p130Cas signaling outputs to E-cadherin turnover, RTK coupling (NRP1-PDGFRα), and mechanical reorientation under cyclic stretch.

    Evidence siRNA/degradation assays for E-cadherin; NRP1 co-IP and mutant migration assays; SYF/siRNA and stretch-sensitive tyrosine mutants under cyclic stretch

    PMID:21306301 PMID:21765937 PMID:22178114

    Open questions at the time
    • E-cadherin degradation mechanism inferred from inhibitors without reconstitution
    • Stretch-sensitive tyrosine identity and force-sensing biophysics not fully defined
  20. 2012 High

    Identified PTPN14 as a specific Tyr128 phosphatase regulating tumor growth and linked p130Cas phosphorylation to actin-dependent myogenic differentiation via MAL/SRF.

    Evidence Phospho-proteomic screen, Y128F knock-in, in vitro phosphatase/kinase assays, xenografts; phosphorylation-defective mutant rescue of myotube formation

    PMID:22587391 PMID:22710723

    Open questions at the time
    • How Y128 phosphorylation feeds AKT not fully detailed
    • MAL nuclear-localization link to specific phospho-sites not mapped
  21. 2013 High

    Defined an osteoclast-specific requirement for p130Cas in actin-ring formation and bone resorption through Dock5-mediated Rac1 activation.

    Evidence Osteoclast-specific conditional knockout with actin-ring/pit assays and Dock5 co-IP

    PMID:23526406

    Open questions at the time
    • How p130Cas promotes Dock5-Src/Pyk2 association not molecularly resolved
    • Whether the mechanism generalizes beyond osteoclasts untested
  22. 2014 High

    Resolved the BCAR3-BCAR1 interaction structurally and showed it drives antiestrogen resistance by raising phospho-BCAR1 and ERK1/2 activity.

    Evidence Structure-based interface mutants with co-IP, resistance assays, and ERK inhibition

    PMID:24584939

    Open questions at the time
    • How the interaction elevates BCAR1 phosphorylation mechanistically unclear
    • In vivo relevance to patient resistance not established
  23. 2019 High

    Revealed two new spatially distinct functions: CCHD-mediated recruitment to filopodia tips as a mechanosensitive stability regulator, and shear-stress-induced nuclear translocation in osteocytes that restrains NF-κB/RANKL-driven bone resorption.

    Evidence SIM localization screen with CCHD deletion and live imaging; osteocyte-specific knockout with nuclear fractionation, translocation imaging, and NF-κB reporters

    PMID:30639111 PMID:31579816

    Open questions at the time
    • Mechanism of nuclear import and direct NF-κB target not defined
    • How CCHD senses force at filopodia tips unresolved
  24. 2021 High

    Placed p130Cas in a SRC-DOCK1-RAC1-β-catenin axis controlling MYC transcription and identified BCAR1 as a synthetic-lethal dependency in mutant-KRAS pancreatic cancer.

    Evidence Genome-scale CRISPR/siRNA screen with pathway inhibitors, MYC reporter, and DOCK1/RAC1/β-catenin epistasis

    PMID:34192548

    Open questions at the time
    • Direct molecular link from RAC1 to β-catenin/MYC not fully reconstituted
    • Therapeutic window of the synthetic-lethal interaction untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the distinct phosphorylation-state-defined conformations of p130Cas are selected by specific upstream cues and translated into the choice between migration, growth control, mechanosensing, and nuclear NF-κB regulation remains unresolved.
  • No structural model of full-length p130Cas in its adhesion complex
  • Mechanism and regulators of nuclear import not identified
  • Direct nuclear binding partners mediating NF-κB suppression unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4 GO:0008092 cytoskeletal protein binding 3 GO:0140299 molecular sensor activity 3
Localization
GO:0005856 cytoskeleton 3 GO:0005886 plasma membrane 2 GO:0005634 nucleus 1
Pathway
R-HSA-162582 Signal Transduction 4 R-HSA-1643685 Disease 4 R-HSA-1266738 Developmental Biology 3 R-HSA-1474244 Extracellular matrix organization 3
Partners
BCAR3CRKDOCK1NCK1PTK2PTPN14PXNSRC
Complex memberships
ERα-c-Src-PI3K complexSrc-FAK-p130Cas focal adhesion complexp130Cas-CrkII complex

Evidence

Reading pass · 53 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 p130Cas (BCAR1) interacts directly with FAK via its SH3 domain binding to the proline-rich region of FAK (APPKPSR, residues 711-717), as demonstrated by yeast two-hybrid screen and confirmed by co-immunoprecipitation in mouse fibroblasts. Yeast two-hybrid screen, co-immunoprecipitation Proceedings of the National Academy of Sciences of the United States of America High 7479864
1995 p130Cas becomes tyrosine-phosphorylated during integrin-mediated cell adhesion to extracellular matrix substrata, dependent on integrin ligation and actin cytoskeleton integrity (blocked by cytochalasin D), but independent of cell adhesion in v-src-transformed cells. Western blot, immunoprecipitation, cytochalasin D inhibition The Journal of biological chemistry High 7545676
1996 The C-terminal region of p130Cas directly binds both SH2 and SH3 domains of Src kinase; the SH3-binding site maps to RPLPSPP near the C-terminus, and both this site and Tyr762 are required for Src association, while the substrate domain mediates binding to v-Crk via its SH2 domain. GST fusion protein pulldown, mutagenesis The Journal of biological chemistry High 8621540
1996 Individual domains of p130Cas associate with distinct binding partners: FAK, v-Src, and v-Crk binding sites are mapped to distinct regions of p130Cas, and many interactions are dependent on the tyrosine-phosphorylation state of p130Cas. In vitro domain-binding assays, domain mapping Oncogene Medium 8649789
1997 FAK phosphorylates p130Cas in vitro and in cells upon fibronectin stimulation (via a FAK/Src 1-298 complex), and FAK-induced phosphorylation of p130Cas promotes SH2-domain-dependent binding of the Nck adaptor protein to p130Cas. In vitro kinase assay, co-immunoprecipitation, fibronectin stimulation Molecular and cellular biology High 9032297
1997 During bacterial uptake, p130Cas and FAK are phosphorylated and recruited to peripheral focal complexes in HeLa cells; Yersinia YopH (active PTPase) dephosphorylates p130Cas and FAK and disrupts these complexes, blocking uptake. Inactive YopH co-localizes with and binds the tyrosine-phosphorylated forms of FAK and p130Cas. Co-localization, immunoprecipitation, PTPase activity assays The EMBO journal High 9171345
1997 RAFTK (Pyk2) directly phosphorylates the substrate domain of p130Cas in vitro; full phosphorylation of p130Cas requires cooperation between RAFTK and Src kinases, with the C-terminal domain of p130Cas requiring RAFTK autophosphorylation site Tyr402 (which mediates Src binding). In vitro kinase assay with purified domains, mutagenesis of Tyr402 The Journal of biological chemistry High 9242628
1998 p130Cas (BCAR1) is a mediator of FAK-promoted cell migration; FAK mutant P712/715A (which reduces p130Cas association) fails to promote migration, while co-expression of p130Cas enhances migration and the p130Cas SH3 domain alone acts as a dominant negative inhibitor of migration. FAK mutant expression, migration assay, co-immunoprecipitation The Journal of cell biology High 9425168
1998 Mice lacking p130Cas (Crkas/BCAR1) die in utero with cardiovascular defects, disorganized myofibrils, and impaired actin stress fiber formation in fibroblasts; activated Src in Cas-deficient fibroblasts fails to induce full transformation due to insufficient actin accumulation in podosomes. Knockout mouse generation, histology, electron microscopy, primary fibroblast analysis Nature genetics High 9697697
1998 Angiotensin II induces tyrosine phosphorylation of p130Cas in vascular smooth muscle cells via a pathway requiring c-Src family kinase activity, intracellular Ca2+, and protein kinase C; phosphorylated p130Cas then associates with protein kinase C-alpha and pp120, with c-Src constitutively associated. Immunoprecipitation, kinase inhibitors, chelators, Western blot Circulation research Medium 9648724
1998 Growth hormone stimulates formation of a multiprotein signaling complex centered on p130Cas and CrkII, including c-Src, c-Fyn, tensin, paxillin, IRS-1, p85/PI3K, C3G, SHC, Grb-2, Sos-1, c-Cbl, and Nck; this complex formation correlates with JNK/SAPK activation. Co-immunoprecipitation, kinase activity assays The Journal of biological chemistry Medium 9837978
1999 PTEN dephosphorylates FAK and decreases tyrosine phosphorylation of both FAK and p130Cas; p130Cas overexpression reverses PTEN inhibition of cell invasion and migration without affecting PTEN-dependent growth suppression, and p130Cas cannot be directly dephosphorylated by PTEN in vitro or shown to interact with PTEN in cells. Overexpression, in vitro phosphatase assay, co-immunoprecipitation, invasion assays Cancer research High 9927060
1999 p130Cas-deficient fibroblasts exhibit significant defects in cell movement, migration toward fibronectin, and cell spreading; re-expression of p130Cas rescues these defects, establishing a direct role for p130Cas in promoting cell motility. Knockout fibroblast re-expression rescue, wound healing assay, transwell migration assay, cell spreading assay Biochemical and biophysical research communications High 10448062
1999 MCSP (melanoma chondroitin sulphate proteoglycan) stimulation recruits tyrosine-phosphorylated p130Cas and activates Cdc42; MCSP-induced cell spreading is dependent on active Cdc42, the Cdc42-associated kinase Ack-1, and tyrosine phosphorylation of p130Cas; Ack-1 and Cdc42 inhibition abrogates MCSP-induced p130Cas phosphorylation. Co-immunoprecipitation, dominant negative constructs, cell spreading assay Nature cell biology Medium 10587647
1999 The transmembrane PTPase LAR dephosphorylates p130Cas in vitro and in vivo, reducing its protein stability and eliminating it from focal adhesions, leading to apoptosis; restoring p130Cas levels alleviates LAR-induced apoptosis, identifying p130Cas as an in vivo substrate of LAR. In vitro phosphatase assay, substrate-trapping mutants, rescue by p130Cas overexpression, immunofluorescence Genes to cells : devoted to molecular & cellular mechanisms High 10320483
2000 p130Cas associates with the p85 subunit of PI 3-kinase through its RPLPSPP proline-rich domain (SH3 domain of p85 binding), and this interaction is required for adenovirus internalization mediated by alpha-v integrins; inhibition of p130Cas phosphorylation or deletion of its substrate domain blocks adenovirus cell entry. Co-immunoprecipitation, dominant negative constructs, adenovirus internalization assay The Journal of biological chemistry High 10799562
2000 p130Cas regulates AND-34 (BCAR3) GEF activity: overexpression of p130Cas (but not an AND-34-binding mutant) inhibits AND-34's Ral GEF activity on RalA, Rap1A, and R-Ras; efficient p130Cas-AND-34 binding requires both the Src-binding domain and a flanking C-terminal region of p130Cas. GEF activity assay in cells, co-immunoprecipitation, mutant binding analysis The Journal of biological chemistry Medium 10896938
2000 BCAR1 (p130Cas) overexpression confers antiestrogen resistance on ZR-75-1 breast cancer cells, identified as the gene responsible for the BCAR1 locus; the gene encodes a 870 amino acid protein homologous to rat p130Cas, located at chromosome 16q23.1. Retroviral mutagenesis, cell fusion, cDNA transfection, functional growth assay Journal of the National Cancer Institute High 10639512
2000 Caspase-3 cleaves p130Cas at two consensus sites DVPD(416)G and DSPD(748)G during etoposide-induced apoptosis; cleavage correlates with loss of FAK from focal adhesions and attenuation of Cas-paxillin interactions, contributing to focal adhesion complex disassembly. In vitro caspase cleavage assay, site-directed mutagenesis, immunofluorescence Molecular biology of the cell High 10712510
2001 Integrin-mediated adhesion induces assembly of a macromolecular complex at the cell membrane containing p130Cas, c-Src, and the EGF receptor; both c-Src and p130Cas are required for integrin-dependent phosphorylation of EGF receptor at specific tyrosines (Y845, Y1068, Y1086, Y1173). Co-immunoprecipitation, dominant negative constructs, phospho-specific antibodies The Journal of biological chemistry Medium 11756413
2001 SHIP2 associates with p130Cas primarily through the SHIP2 SH2 domain; an SH2 domain mutant (R47G) abolishes this interaction and increased SHIP2 expression promotes adhesion, while a catalytic domain deletion mutant inhibits cell spreading. Co-immunoprecipitation, SH2 domain mutant, cell adhesion and spreading assays Molecular and cellular biology Medium 11158326
2001 FAK and p130Cas localize to sarcomeric Z-lines in cardiac myocytes; expression of the p130Cas-binding proline-rich region 1 of FAK disrupts Cas-FAK association and impairs sarcomere structural stability; Cas alone or with Src modulates atrial natriuretic peptide (ANP) gene promoter activity as a marker of cardiac hypertrophy. Immunofluorescence localization, dominant negative expression, luciferase reporter assay Molecular biology of the cell Medium 11514617
2001 SAP-1 phosphatase dephosphorylates p130Cas in intact cells and preferentially in vitro; a substrate-trapping SAP-1 mutant induces hyperphosphorylation of p130Cas (dominant negative effect); SAP-1 overexpression inhibits cell spreading on fibronectin and colony formation. Substrate-trapping mutagenesis, in vitro phosphatase assay, immunoprecipitation, spreading assay The Journal of biological chemistry High 11278335
2001 p130Cas localizes to focal adhesions in podocytes and colocalizes with F-actin at stress fiber ends, distinct from CD2AP which colocalizes with dynamic F-actin at lamellipodia. Immunofluorescence, immunoelectron microscopy American journal of physiology. Renal physiology Medium 11553524
2002 EphrinA1-induced cytoskeletal reorganization and cell spreading in NIH3T3 cells requires both FAK and p130Cas; MEFs from FAK-/- and p130Cas-/- mice have severe defects in ephrinA1-induced spreading, reversed by re-expression of FAK or p130Cas respectively; EphA2, FAK, and p130Cas are the major ephrin-dependent phosphotyrosyl proteins. Knockout MEFs, re-expression rescue, phosphotyrosine analysis, cell spreading assay Nature cell biology High 12134157
2003 The p130CAS-CrkII complex functions as a molecular switch in cell motility; KAI1/CD82 expression decreases p130CAS protein levels, reduces p130CAS-CrkII complex formation, and inhibits migration; overexpression of p130CAS in KAI1/CD82-expressing cells restores complex formation and largely reverses migration inhibition. Co-immunoprecipitation, overexpression rescue, migration assay The Journal of biological chemistry Medium 12738793
2003 Bmx/Etk kinase interacts with p130Cas at membrane ruffles, phosphorylates p130Cas, and promotes Cas-Crk complex formation; a Bmx mutant failing to interact with Cas also fails to induce cell migration; dominant-negative Cas (unable to bind Crk) inhibits Bmx-induced membrane ruffling and migration. Co-immunoprecipitation, dominant negative constructs, membrane ruffling and migration assays The Journal of biological chemistry Medium 12832404
2003 p140Cap (Cas-associated protein) directly interacts with p130Cas through the p140Cap carboxy-terminal region; the two proteins co-immunoprecipitate and colocalize in lamellipodia; p140Cap overexpression delays cell spreading on fibronectin. Affinity chromatography, mass spectrometry, co-immunoprecipitation, immunofluorescence, cell spreading assay Molecular biology of the cell Medium 14657239
2004 p130Cas rapidly associates with estrogen receptor alpha (ERα) in a multi-molecular complex containing c-Src and p85/PI3K within 3 minutes of estrogen treatment; this association is c-Src-dependent; p130Cas overexpression enhances estrogen-dependent Src kinase and ERK1/2 activities, while siRNA silencing of p130Cas inhibits estrogen-dependent ERK1/2 activity and cyclin D1 induction. Co-immunoprecipitation, siRNA knockdown, kinase activity assays, reporter assays Journal of cell science High 15020686
2005 Ajuba (LIM protein) associates with the focal adhesion-targeting domain of p130Cas and acts upstream of p130Cas to localize it to nascent adhesive sites; Ajuba-null cells have reduced p130Cas, Crk, Dock180, and FAK at nascent focal complexes and blunted Rac activation in response to migratory cues. Co-immunoprecipitation, knockout mouse fibroblasts, FRET analysis of Rac activation, rescue experiments The Journal of cell biology High 15728191
2005 p130Cas overexpression in mammary gland (MMTV-p130Cas mice) leads to mammary epithelial hyperplasia associated with activation of Src, ERK1/2, and Akt; crossing with MMTV-HER2-Neu mice accelerates multifocal mammary tumor development with increased Src and Akt activation; p130Cas siRNA increases apoptosis in HER2-Neu-expressing cells. Transgenic mouse models, signaling pathway analysis, siRNA knockdown, tumor latency measurement Cancer research High 16651418
2005 NPM-ALK (anaplastic lymphoma kinase fusion) binds p130Cas and induces its phosphorylation in a manner dependent on ALK kinase activity and the adaptor Grb2; p130Cas-/- fibroblasts expressing NPM-ALK show impaired actin depolymerization and are no longer transformed, establishing p130Cas as essential for ALK-mediated transformation. Mass spectrometry identification, co-immunoprecipitation, kinase-dead mutant, Grb2 dominant negative, Cas-/- fibroblast reconstitution Blood High 16105984
2006 Fibronectin matrix rigidity response requires Fyn (but not endogenous c-Src) recruitment to early adhesions; p130Cas is also required for the rigidity response and is phosphorylated at the leading edge in a Fyn-dependent manner, suggesting force-dependent Fyn phosphorylation of p130Cas. Live cell imaging, dominant negative mutants, phosphorylation site analysis, leading edge localization Molecular biology of the cell Medium 16597701
2006 Ack1 mediates Cdc42-dependent cell migration through p130Cas: Ack1 forms a signaling complex with Cdc42, p130Cas, and Crk via SH3 domain interactions; Ack1 phosphorylates the substrate domain of p130Cas; siRNA knockdown of either p130Cas or Ack1 blocks Cdc42-induced migration. Co-immunoprecipitation, siRNA knockdown, migration assay, phosphorylation analysis The Journal of biological chemistry High 17038317
2007 p130CAS is required for netrin-1 signaling: netrin-1 induces p130CAS tyrosine phosphorylation downstream of Src family kinases and upstream of Rac1/Cdc42; inhibiting p130CAS signaling blocks netrin-1-promoted neurite outgrowth and axon attraction; p130CAS RNAi causes defects in commissural axon projection in vivo. Biochemical epistasis, RNAi knockdown, in vivo commissural axon projection analysis The Journal of neuroscience : the official journal of the Society for Neuroscience High 17251438
2008 Tyrosine phosphorylation of p130Cas is sufficient to activate Rac1 and induce membrane ruffling/lamellipodium formation in SYF cells; the kinase activity of Src (not FAK) is essential for phosphorylating p130Cas within the Src-FAK-p130Cas trimolecular complex at focal adhesions. Functional Interaction Trap (FIT) method for selective substrate phosphorylation, Rac1 activation assay, dominant negative FAK BMC cell biology High 18793427
2008 Once tyrosine-phosphorylated via integrin signaling, p130Cas binds to Smad3 and reduces Smad3 phosphorylation, inhibiting TGF-β-mediated growth arrest; loss of p130Cas abrogates integrin-mediated suppression of TGF-β/Smad3 signaling. Co-immunoprecipitation, loss/gain of function, Smad3 phosphorylation assay, cell cycle analysis Molecular biology of the cell High 18321991
2009 Estrogen-dependent formation of ERα-BCAR1 complexes (within ~5 min) is increased by RANKL co-treatment; BCAR1 associates with the RANKL signaling intermediate Traf6 in this complex; BCAR1 siRNA knockdown abolishes estradiol inhibition of osteoclast differentiation, demonstrating BCAR1 is required for estrogen's non-genomic inhibition of NF-κB-mediated osteoclastogenesis. Co-immunoprecipitation, siRNA knockdown, osteoclast differentiation assay, NF-κB localization Experimental cell research Medium 19331827
2010 Both the N-terminal SH3 domain and the C-terminal Cas-family homology (CCH) domain of p130Cas are required for its localization to focal adhesions (FA targeting requires FAK for the SH3 domain function); deletion of either domain impairs FA localization and tyrosine phosphorylation; both domains are necessary for p130Cas to promote cell migration. Live cell microscopy, FRAP, deletion mutants, FA targeting analysis The Journal of biological chemistry High 20430882
2010 p130Cas substrate domain (SD) phosphorylation is the sole p130Cas signaling function for promoting leading-edge actin flux and FA assembly/disassembly rates, while the Src-binding domain (SBD) has a distinct function in sustained FA disassembly and cell migration that is independent of its known role in promoting SD phosphorylation. Cas-/- MEF rescue with domain mutants, quantitative live cell imaging of FA dynamics and actin flux PloS one High 20976150
2010 p130Cas is an essential transducer in ErbB2 transformation: p130Cas forms a functional complex with ErbB2, c-Src, and FAK in transformed cells; p130Cas silencing impairs foci formation, anchorage-independent growth, and in vivo tumor growth; p130Cas overexpression with ErbB2 activates Rac1 and MMP9 secretion, conferring invasive properties. siRNA knockdown, in vivo tumor growth, co-immunoprecipitation, Rac1 activation assay, invasion assay FASEB journal High 20505116
2011 BCAR1/p130Cas and NEDD9 signal through SRC to promote E-cadherin removal from the cell membrane and lysosomal degradation, negatively regulating E-cadherin expression in human mammary cells without affecting E-cadherin transcription. siRNA knockdown, inhibitor studies, pulse-chase/degradation assay, in vivo mammary tumor analysis PloS one Medium 21765937
2011 NRP1 (neuropilin-1) co-immunoprecipitates with PDGFRα and selectively mediates PDGF-induced tyrosine phosphorylation of p130Cas; NRP1 siRNA and NRP1 mutants lacking the intracellular domain inhibit PDGF-induced p130Cas phosphorylation and VSMC migration; p130Cas knockdown also inhibits HCASMC migration. Co-immunoprecipitation, siRNA knockdown, adenoviral overexpression of NRP1 mutants, migration assays The Biochemical journal Medium 21306301
2012 PTPN14 directly dephosphorylates p130Cas specifically at tyrosine residue Y128; homozygous Y128F knock-in CRC cells show reduced migration, colony formation, and slower xenograft tumor growth with decreased AKT phosphorylation; SRC phosphorylates p130Cas Y128. Phospho-proteomic screen, knock-in mutagenesis, in vitro phosphatase/kinase assay, xenograft tumor growth Oncogene High 22710723
2012 p130Cas-dependent actin remodeling regulates myogenic differentiation: p130Cas phosphorylation (mediated by integrin β3) promotes F-actin formation, nuclear localization of MAL/SRF co-activator, and myotube formation; phosphorylation-defective p130Cas mutant fails to rescue these processes; p130Cas phosphorylation prevents cofilin activation. siRNA knockdown, phosphorylation-defective mutant re-expression, F-actin staining, nuclear MAL localization assay The Biochemical journal High 22587391
2013 In osteoclasts, p130Cas is required for actin ring formation and bone resorption; p130Cas conditional knockout osteoclasts show reduced Rac1 activity, disrupted Rac1 distribution, and failure of Dock5 (a Rac GEF) to associate with Src or Pyk2, while initial β3-integrin and Src phosphorylation events are intact. Osteoclast-specific conditional knockout, actin ring assay, pit formation assay, co-immunoprecipitation of Dock5 Journal of bone and mineral research High 23526406
2014 BCAR1 and BCAR3 bind tightly through their C-terminal domains; structure-based interaction-disrupting mutants show that BCAR3-induced antiestrogen resistance critically depends on binding to BCAR1; BCAR3-BCAR1 interaction increases phosphorylated BCAR1 levels and ERK1/2 activity, which mediates resistance. Structure-based mutagenesis of interaction interface, co-immunoprecipitation, antiestrogen resistance functional assay, ERK inhibition The Journal of biological chemistry High 24584939
2019 p130Cas (BCAR1) is recruited to filopodia tips via its C-terminal Cas family homology domain (CCHD) and acts as a mechanosensitive regulator of filopodia stability, as demonstrated by structured-illumination microscopy mapping and live imaging. SIM-based protein localization screen, live imaging, domain deletion analysis (CCHD) Current biology : CB High 30639111
2019 p130Cas (Cas) translocates to the nucleus in osteocytes in response to fluid shear stress and alleviates NF-κB activity; osteocyte-specific Cas deficiency increases NF-κB-mediated RANKL expression and osteoclastic bone resorption, leading to osteopenia; shear stress-dependent nuclear Cas translocation was directly observed. Osteocyte-specific conditional knockout, nuclear fractionation, live-cell imaging of Cas translocation, NF-κB reporter assays Science advances High 31579816
2021 SRC-inhibitor-mediated suppression of p130Cas phosphorylation impairs MYC transcription through a DOCK1-RAC1-β-catenin-dependent mechanism; BCAR1 siRNA sensitizes pancreatic cancer cells to ERK inhibition, identifying BCAR1 as a synthetic lethal interactor with mutant KRAS. siRNA/CRISPR genome-scale screen, pathway inhibitors, MYC reporter, DOCK1/RAC1/β-catenin epistasis Cell reports High 34192548
2006 Salmonella typhimurium invasion of host cells requires p130Cas; Cas-/- cells are impaired in bacterial internalization, and reconstitution requires the central Cas YXXP repeat domain; FAK overexpression suppresses the invasion defect in Cas-/- cells, suggesting a functional FAK-Cas link in Salmonella invasion. Knockout MEFs, reconstitution with domain mutants, genetic suppressor analysis Molecular biology of the cell High 16914515
2011 Cyclic stretch induces cell reorientation in a Src family kinase- and p130Cas-dependent manner; knockout or knockdown of p130Cas reduces reorientation upon cyclic stretch, and mutation of stretch-sensitive tyrosines of p130Cas produces identical impairment. SYF knockout MEFs, siRNA knockdown, p130Cas tyrosine mutants, cyclic stretch device European journal of cell biology High 22178114
2004 p130Cas cleavage occurs during anoikis; overexpression of the p130Cas cleavage product induces apoptosis; caspases and calpain are both involved in cleavage, though additional unidentified proteases may also cleave p130Cas at the early stage of anoikis. Cell detachment-induced anoikis assay, cleavage fragment overexpression, caspase/calpain inhibitors Journal of cellular biochemistry Medium 14743392

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1998 Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration. The Journal of cell biology 422 9425168
1997 Fibronectin-stimulated signaling from a focal adhesion kinase-c-Src complex: involvement of the Grb2, p130cas, and Nck adaptor proteins. Molecular and cellular biology 406 9032297
1995 Interaction between focal adhesion kinase and Crk-associated tyrosine kinase substrate p130Cas. Proceedings of the National Academy of Sciences of the United States of America 390 7479864
1999 Tumor suppressor PTEN inhibition of cell invasion, migration, and growth: differential involvement of focal adhesion kinase and p130Cas. Cancer research 328 9927060
2001 Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines. The Journal of biological chemistry 317 11756413
1998 Cardiovascular anomaly, impaired actin bundling and resistance to Src-induced transformation in mice lacking p130Cas. Nature genetics 309 9697697
1997 The PTPase YopH inhibits uptake of Yersinia, tyrosine phosphorylation of p130Cas and FAK, and the associated accumulation of these proteins in peripheral focal adhesions. The EMBO journal 297 9171345
1995 Tyrosine phosphorylation of p130Cas and cortactin accompanies integrin-mediated cell adhesion to extracellular matrix. The Journal of biological chemistry 275 7545676
2006 p130Cas: a versatile scaffold in signaling networks. Trends in cell biology 266 16581250
1996 Human enhancer of filamentation 1, a novel p130cas-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae. Molecular and cellular biology 219 8668148
1996 Direct binding of C-terminal region of p130Cas to SH2 and SH3 domains of Src kinase. The Journal of biological chemistry 216 8621540
1999 Melanoma chondroitin sulphate proteoglycan regulates cell spreading through Cdc42, Ack-1 and p130cas. Nature cell biology 172 10587647
2002 EphrinA1-induced cytoskeletal re-organization requires FAK and p130(cas). Nature cell biology 158 12134157
2005 Ganglioside GD3 promotes cell growth and invasion through p130Cas and paxillin in malignant melanoma cells. Proceedings of the National Academy of Sciences of the United States of America 140 16040804
2000 BCAR1, a human homologue of the adapter protein p130Cas, and antiestrogen resistance in breast cancer cells. Journal of the National Cancer Institute 129 10639512
2011 Neuropilin-1 mediates PDGF stimulation of vascular smooth muscle cell migration and signalling via p130Cas. The Biochemical journal 120 21306301
2002 Tyrosine phosphorylation of paxillin, FAK, and p130CAS: effects on cell spreading and migration. Frontiers in bioscience : a journal and virtual library 120 11779709
2006 p130Cas as a new regulator of mammary epithelial cell proliferation, survival, and HER2-neu oncogene-dependent breast tumorigenesis. Cancer research 113 16651418
2001 SH2-containing inositol 5'-phosphatase SHIP2 associates with the p130(Cas) adapter protein and regulates cellular adhesion and spreading. Molecular and cellular biology 108 11158326
1999 p130(Cas), an assembling molecule of actin filaments, promotes cell movement, cell migration, and cell spreading in fibroblasts. Biochemical and biophysical research communications 107 10448062
1997 The related adhesion focal tyrosine kinase differentially phosphorylates p130Cas and the Cas-like protein, p105HEF1. The Journal of biological chemistry 107 9242628
2004 p130Cas interacts with estrogen receptor alpha and modulates non-genomic estrogen signaling in breast cancer cells. Journal of cell science 103 15020686
2005 The LIM protein Ajuba influences p130Cas localization and Rac1 activity during cell migration. The Journal of cell biology 97 15728191
2000 Association of p130CAS with phosphatidylinositol-3-OH kinase mediates adenovirus cell entry. The Journal of biological chemistry 94 10799562
2006 Fibronectin rigidity response through Fyn and p130Cas recruitment to the leading edge. Molecular biology of the cell 91 16597701
2001 Nephrocystin interacts with Pyk2, p130(Cas), and tensin and triggers phosphorylation of Pyk2. Proceedings of the National Academy of Sciences of the United States of America 90 11493697
1996 The identification of p130cas-binding proteins and their role in cellular transformation. Oncogene 90 8649789
2019 Filopodome Mapping Identifies p130Cas as a Mechanosensitive Regulator of Filopodia Stability. Current biology : CB 89 30639111
2014 Mechanosensors in integrin signaling: the emerging role of p130Cas. European journal of cell biology 89 25062607
2000 p130Cas regulates the activity of AND-34, a novel Ral, Rap1, and R-Ras guanine nucleotide exchange factor. The Journal of biological chemistry 86 10896938
2001 Focal adhesion kinase and p130Cas mediate both sarcomeric organization and activation of genes associated with cardiac myocyte hypertrophy. Molecular biology of the cell 80 11514617
2008 Chondroitin sulphate-modified neuropilin 1 is expressed in human tumour cells and modulates 3D invasion in the U87MG human glioblastoma cell line through a p130Cas-mediated pathway. EMBO reports 79 18704117
2005 Polarized downregulation of the paxillin-p130CAS-Rac1 pathway induced by shear flow. Journal of cell science 79 16129884
1998 Reactive oxygen species activate focal adhesion kinase, paxillin and p130cas tyrosine phosphorylation in endothelial cells. Free radical biology & medicine 79 9870555
2001 CD2AP and p130Cas localize to different F-actin structures in podocytes. American journal of physiology. Renal physiology 78 11553524
2012 p130Cas: a key signalling node in health and disease. Cellular signalling 77 23277200
2012 A critical role for p130Cas in the progression of pulmonary hypertension in humans and rodents. American journal of respiratory and critical care medicine 76 22798315
2009 Estrogen inhibits RANKL-stimulated osteoclastic differentiation of human monocytes through estrogen and RANKL-regulated interaction of estrogen receptor-alpha with BCAR1 and Traf6. Experimental cell research 73 19331827
2003 Requirement of the p130CAS-Crk coupling for metastasis suppressor KAI1/CD82-mediated inhibition of cell migration. The Journal of biological chemistry 73 12738793
1998 Growth hormone stimulates the formation of a multiprotein signaling complex involving p130(Cas) and CrkII. Resultant activation of c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK). The Journal of biological chemistry 69 9837978
2000 Caspase-mediated cleavage of p130cas in etoposide-induced apoptotic Rat-1 cells. Molecular biology of the cell 68 10712510
2011 NEDD9 and BCAR1 negatively regulate E-cadherin membrane localization, and promote E-cadherin degradation. PloS one 67 21765937
1999 Rho-dependent and -independent tyrosine phosphorylation of focal adhesion kinase, paxillin and p130Cas mediated by Ret kinase. Oncogene 67 10208419
2007 Activation of the FAK-src molecular scaffolds and p130Cas-JNK signaling cascades by alpha1-integrins during colon cancer cell invasion. International journal of oncology 65 17982677
2007 Breast cancer antiestrogen resistance-3 expression regulates breast cancer cell migration through promotion of p130Cas membrane localization and membrane ruffling. Cancer research 61 17616674
2005 p130Cas mediates the transforming properties of the anaplastic lymphoma kinase. Blood 58 16105984
2008 Phosphorylation of p130Cas initiates Rac activation and membrane ruffling. BMC cell biology 57 18793427
2003 P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading. Molecular biology of the cell 56 14657239
1998 Phosphorylation of p130Cas by angiotensin II is dependent on c-Src, intracellular Ca2+, and protein kinase C. Circulation research 55 9648724
2006 Ack1 mediates Cdc42-dependent cell migration and signaling to p130Cas. The Journal of biological chemistry 53 17038317
2003 p130Cas Couples the tyrosine kinase Bmx/Etk with regulation of the actin cytoskeleton and cell migration. The Journal of biological chemistry 53 12832404
1999 Transmembrane tyrosine phosphatase LAR induces apoptosis by dephosphorylating and destabilizing p130Cas. Genes to cells : devoted to molecular & cellular mechanisms 53 10320483
2013 Type 2 diabetes risk alleles near BCAR1 and in ANK1 associate with decreased β-cell function whereas risk alleles near ANKRD55 and GRB14 associate with decreased insulin sensitivity in the Danish Inter99 cohort. The Journal of clinical endocrinology and metabolism 52 23457408
2000 A switch from p130Cas/Crk to Gab1/Crk signaling correlates with anchorage independent growth and JNK activation in cells transformed by the Met receptor oncoprotein. Oncogene 52 11146548
2007 p130CAS is required for netrin signaling and commissural axon guidance. The Journal of neuroscience : the official journal of the Society for Neuroscience 51 17251438
1996 Networks of interaction of p120cbl and p130cas with Crk and Grb2 adaptor proteins. Oncogene 50 8700507
2010 Dynamics and mechanism of p130Cas localization to focal adhesions. The Journal of biological chemistry 47 20430882
2003 Selective involvement of p130Cas/Crk/Pyk2/c-Src in endothelin-1-induced JNK activation. Hypertension (Dallas, Tex. : 1979) 47 12719447
2010 P130Cas Src-binding and substrate domains have distinct roles in sustaining focal adhesion disassembly and promoting cell migration. PloS one 46 20976150
1999 AND-34, a novel p130Cas-binding thymic stromal cell protein regulated by adhesion and inflammatory cytokines. Journal of immunology (Baltimore, Md. : 1950) 45 10438950
2008 The integrin-coupled signaling adaptor p130Cas suppresses Smad3 function in transforming growth factor-beta signaling. Molecular biology of the cell 44 18321991
2011 Silencing of p130cas in ovarian carcinoma: a novel mechanism for tumor cell death. Journal of the National Cancer Institute 43 21957230
2006 Tamoxifen treatment promotes phosphorylation of the adhesion molecules, p130Cas/BCAR1, FAK and Src, via an adhesion-dependent pathway. Oncogene 43 16799644
2001 Inhibition of cell growth and spreading by stomach cancer-associated protein-tyrosine phosphatase-1 (SAP-1) through dephosphorylation of p130cas. The Journal of biological chemistry 43 11278335
2013 p130Cas, Crk-associated substrate, plays important roles in osteoclastic bone resorption. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 41 23526406
2010 p130Cas is an essential transducer element in ErbB2 transformation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 41 20505116
2006 Invasion of host cells by Salmonella typhimurium requires focal adhesion kinase and p130Cas. Molecular biology of the cell 41 16914515
1999 Cytoskeleton-dependent tyrosine phosphorylation of the p130(Cas) family member HEF1 downstream of the G protein-coupled calcitonin receptor. Calcitonin induces the association of HEF1, paxillin, and focal adhesion kinase. The Journal of biological chemistry 41 10455189
2007 Focal adhesion kinase as well as p130Cas and paxillin is crucially involved in the enhanced malignant properties under expression of ganglioside GD3 in melanoma cells. Biochimica et biophysica acta 40 18078823
2000 BCAR1/p130Cas expression in untreated and acquired tamoxifen-resistant human breast carcinomas. International journal of cancer 40 11008210
2021 Suppression of macrophage migration by down-regulating Src/FAK/P130Cas activation contributed to the anti-inflammatory activity of sinomenine. Pharmacological research 38 33617975
2015 p130Cas/BCAR1 scaffold protein in tissue homeostasis and pathogenesis. Gene 38 25727852
2011 Integrin αβ1, αvβ, α6β effectors p130Cas, Src and talin regulate carcinoma invasion and chemoresistance. Biochemical and biophysical research communications 38 21291860
2020 CSRP2 suppresses colorectal cancer progression via p130Cas/Rac1 axis-meditated ERK, PAK, and HIPPO signaling pathways. Theranostics 37 33042270
2016 Imatinib and Nilotinib increase glioblastoma cell invasion via Abl-independent stimulation of p130Cas and FAK signalling. Scientific reports 36 27293031
2004 Chemokine receptor CCR6 transduces signals that activate p130Cas and alter cAMP-stimulated ion transport in human intestinal epithelial cells. American journal of physiology. Cell physiology 36 15483227
2001 Degradation of focal adhesion proteins paxillin and p130cas by caspases or calpains in apoptotic rat-1 and L929 cells. Biochemical and biophysical research communications 35 11511102
1998 Protein kinase C-dependent tyrosine phosphorylation of p130cas in differentiating neuroblastoma cells. The Journal of biological chemistry 35 9442079
2012 Identification and functional characterization of p130Cas as a substrate of protein tyrosine phosphatase nonreceptor 14. Oncogene 34 22710723
2004 Cleavage of p130Cas in anoikis. Journal of cellular biochemistry 34 14743392
2017 The miR-24-3p/p130Cas: a novel axis regulating the migration and invasion of cancer cells. Scientific reports 33 28337997
2019 Mechanical regulation of bone homeostasis through p130Cas-mediated alleviation of NF-κB activity. Science advances 32 31579816
2007 BCAR1 expression in prostate cancer: association with 16q23 LOH status, tumor progression and EGFR/KAI1 staining. The Prostate 32 17192874
2021 Targeting p130Cas- and microtubule-dependent MYC regulation sensitizes pancreatic cancer to ERK MAPK inhibition. Cell reports 31 34192548
2013 Cysteine-rich protein 2 alters p130Cas localization and inhibits vascular smooth muscle cell migration. Cardiovascular research 30 23975851
2005 p130cas but not paxillin is essential for Caco-2 intestinal epithelial cell spreading and migration on collagen IV. The Journal of biological chemistry 30 15817476
2013 Acquisition of the metastatic phenotype is accompanied by H2O2-dependent activation of the p130Cas signaling complex. Molecular cancer research : MCR 29 23345605
2002 Human endothelial Pyk2 is expressed in two isoforms and associates with paxillin and p130Cas. Biochemical and biophysical research communications 29 11820787
2014 Association of the breast cancer antiestrogen resistance protein 1 (BCAR1) and BCAR3 scaffolding proteins in cell signaling and antiestrogen resistance. The Journal of biological chemistry 27 24584939
2001 The integrin alpha 7 cytoplasmic domain regulates cell migration, lamellipodia formation, and p130CAS/Crk coupling. The Journal of biological chemistry 27 11278916
1998 Tyrosine phosphorylation and association of p130Cas and c-Crk II by ANG II in vascular smooth muscle cells. The American journal of physiology 27 9575907
1996 Nerve growth factor induces a multimeric TrkA receptor complex in neuronal cells that includes Crk, SHC and PLC-gamma 1 but excludes P130CAS. Oncogene 27 8552402
2009 Pyk2 mediates endothelin-1 signaling via p130Cas/BCAR3 cascade and regulates human glomerular mesangial cell adhesion and spreading. Journal of cellular physiology 26 19086031
2004 Breast cancer oestrogen independence mediated by BCAR1 or BCAR3 genes is transmitted through mechanisms distinct from the oestrogen receptor signalling pathway or the epidermal growth factor receptor signalling pathway. Breast cancer research : BCR 26 15642172
2012 p130Cas-dependent actin remodelling regulates myogenic differentiation. The Biochemical journal 24 22587391
2009 Propofol inhibits pressure-stimulated macrophage phagocytosis via the GABAA receptor and dysregulation of p130cas phosphorylation. American journal of physiology. Cell physiology 24 19357231
2005 Interactions of the integrin subunit beta1A with protein kinase B/Akt, p130Cas and paxillin contribute to regulation of radiation survival. Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 24 16024122
2022 P130cas-FAK interaction is essential for YAP-mediated radioresistance of non-small cell lung cancer. Cell death & disease 23 36088346
2011 p130Cas, E-cadherin and β-catenin in human transitional cell carcinoma of the bladder: expression and clinicopathological significance. International journal of urology : official journal of the Japanese Urological Association 23 21672035
2011 Cyclic stretch induces reorientation of cells in a Src family kinase- and p130Cas-dependent manner. European journal of cell biology 23 22178114

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